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1.
International Eye Science ; (12): 163-166, 2022.
Article in Chinese | WPRIM | ID: wpr-906755

ABSTRACT

@#AIM: To analyze the role of preoperative telephone interview in the cancellation rate for ophthalmic daytime surgery. <p>METHODS: A prospective, single center study was conducted to compare and analyze the cancellation of daytime surgery in two time periods. The control group was from June 2018 to June 2019, only routine nursing and health education were implemented. The patient in the intervention group were from October 2019 to October 2020, add the telephone interview of the day before operation was added by the nurses in the ophthalmic operating room. <p>RESULTS: The main reasons for canceling the operation included upper respiratory tract infection, abnormal blood glucose, abnormal blood pressure and physiological period, abnormal preoperative examination results, and patients didn't follow the doctor's advice to use preoperative eye drops. In the control group, there were 16 974 cases scheduled for daytime operation, 16 332 cases actually operated, and the cancellation rate of operation was 3.78%(642 cases cancelled). In the intervention group, 17 694 cases were scheduled for daytime operation, and 17 296 cases were actually operated, with the cancellation rate of 2.25%(398 cases cancelled). The disease distribution and cancellation reasons of the two groups were statistically significant(<i>P</i><0.05).<p>CONCLUSION: Telephone interview can effectively reduce the cancellation rate of ophthalmic daytime operation, provide targeted and personalized pre-hospital guidance for patients, improve the compliance of patients and their families to complete preoperative preparation, reduce the psychological anxiety of patients before operation, and improve the medical experience of patients.

2.
International Eye Science ; (12): 554-559, 2022.
Article in Chinese | WPRIM | ID: wpr-922850

ABSTRACT

@#AIM:To provide a clinical reference for the design of personalized surgical parameters for cataract, swept source optic coherence tomography angiography(SS-OCTA)was applied to comparatively study the blood flow of the macular area before operation in patients with different intraocular pressure during cataract surgery.METHODS: Prospective randomized controlled study. A total of 61 patients(77 eyes)who underwent cataract surgery in Sichuan Provincial People's Hospital from January to April 2021 were collected and divided into group A(37 eyes)and group B(40 eyes)for the study using a random number table. Group A and group B received the height of 75 and 90cm perfusion bottles with intraoperative,corresponding to 55.5 and 66.6mmHg intraoperative mean intraocular pressure,respectively. All patients were examined with SS-OCTA in macula area before and 1,7 and 30d after surgery, except routine ophthalmologic examination, the macular retinal thickness, the perfusion area and vessel density of the radial perioptic capillary plexus(RPCP)layer, superficial vascular plexus(SVP)layer, intermediate vascular plexus(IVP)layer and deep vascular plexus(DVP)layer were observed.RESULTS: There were no significant differences in the best corrected visual acuity(BCVA), intraocular pressure, foveal avascular zone(FAZ), macular retinal thickness, perfusion area and vascular density between the two groups at each time point before and after surgery(all <i>P</i>>0.05),but there were differences in time(all <i>P</i><0.05). In both groups, BCVA improved significantly from preoperative values at all time points after surgery, intraocular pressure and FAZ decreased compared with preoperative values(all <i>P</i><0.05), and macular retinal thickness, perfusion area in all layers, and blood flow density were increased compared with preoperative values.CONCLUSION:The macular retinal thickness, blood flow density and perfusion area of all layers increased,and the FAZ area decreased after cataract surgery, which may help to promote the recovery of visual acuity after surgery. At different intraocular pressures of 55.5 and 66.6mmHg intraoperatively, there was no significant difference in the patients'postoperative fundus blood flow changes, therefore, intraocular pressure can be flexibly selected during the operation to provide patients with personalized surgical design.

3.
Article in Chinese | WPRIM | ID: wpr-881490

ABSTRACT

Objective:To evaluate the epidemiological trend of varicella(chickenpox)in Urumqi, this retrospective study was performed by using the school surveillance data between 2014 and 2018. Methods:Descriptive epidemiology method was used to analyze the data of varicella cases from school infectious disease prevention and control information management system from 2014 to 2018. Results:A total of 4 910 cases of varicella were reported in primary and secondary schools, with an average incidence of 290.73/100 000 per year. Primary school students accounted for 60.6%, middle school students for 27.4% and high school students for 12.0%. Among them, there were 2 595 boys and 2 315 girls with sex ratio of 1.12∶1. Two seasonal peaks of varicella incidence were observed every year, from March to June and from November to January of next year. Conclusion:Primary schools are the major places of varicella outbreak and should be the priority places for prevention and control, but the prevention and education in boarding schools should not be neglected. In areas with high incidence of varicella, it is vital to focus on prevention to avoid the infectious diseases spreading and outbreak.

4.
Acta Pharmaceutica Sinica ; (12): 2119-2126, 2021.
Article in Chinese | WPRIM | ID: wpr-887044

ABSTRACT

The purpose of this study was to explore the interaction mechanism between glycyrrhiza protein and berberine in the decocting process of Rhizoma Coptidis and Liquorice and its effect on the pharmacodynamic effect. In this experiment, licorice crude protein was obtained from licorice decoction pieces, and it was found that licorice crude protein and berberine could form spherical supramolecular particles after decocting together. Morphological characterization was carried out by using Malvin particle size analyzer and emission scanning electron microscopy, and the supramolecular particles were observed to be nanoscale, which was significantly different from the morphology of licorice protein and berberine. The results of ultraviolet, infrared and fluorescence spectroscopy showed that the mechanism of molecular interaction was induced by weak bonds such as electrostatic attraction and hydrophobic interaction. Furthermore, the antimicrobial activity of berberine was significantly affected by the supramolecular particles of licorice protein-berberine, which were significantly different from the mechanical mixture. This study reveals the pharmacological value of macromolecular substances such as proteins in the decoction of licorice and Coptis chinensis from a new perspective, which is helpful to promote the secondary development of clinical effective prescriptions, especially the research on the pharmacological substance basis of classic famous prescriptions.

5.
Article in Chinese | WPRIM | ID: wpr-877664

ABSTRACT

OBJECTIVE@#To observe the effect of conventional acupuncture combined with row-like puncture at sternocleidomastoid on peripheral facial palsy at recovery stage.@*METHODS@#A total of 60 patients with peripheral facial palsy at recovery stage were randomized into an observation group and a control group, 30 cases in each one. Acupuncture was applied at affected Cuanzhu (BL 2), Yangbai (GB 14), Sibai (ST 2), Quanliao (SI 18), Jiache (ST 6), Dicang (ST 4), Hegu (LI 4), Taichong (LR 3) and Zusanli (ST 36) in the control group. On the basis of the treatment in the control group, row-like puncture was applied at sternocleidomastoid (1 needle was punctured at muscle origin and insertion respectively, 3 to 4 needles were row-like punctured at the connection line of muscle origin and insertion). The treatment was given once a day, 5 times were as one course, with 2-day interval, totally 4 courses were required in the both groups. The house-brackmann (H-B) facial nerve function grade, facial nerve function rating system-dynamic view rating scale score and facial disability index (FDI) scale score [including scores of FDI physical function (FDIp) and FDI social life function (FDIs)] before and after treatment were observed, and the clinical efficacy was evaluated in the two groups.@*RESULTS@#After treatment, the H-B facial nerve function grades were improved compared before treatment in the both groups (@*CONCLUSION@#Compared with conventional acupuncture, combination therapy with row-like puncture at sternocleidomastoid can improve the therapeutic effect of peripheral facial palsy at recovery stage.


Subject(s)
Acupuncture Points , Acupuncture Therapy , Facial Paralysis/therapy , Humans , Needles , Punctures , Treatment Outcome
6.
Article in Chinese | WPRIM | ID: wpr-905358

ABSTRACT

Coronavirus disease 2019 brought some risks to the current environment of old-age care institutions, the provision of integrated medical care and nursing services, and the psychology of the elderly. To prevent the risks faced by the suitable environment, it is necessary to carry out architectural planning and design, and transform the suitable environment, to achieve the goal of closed management; quarantine areas, clean areas, and polluted areas should be set up to ensure the quality of life. To prevent the risks related to the integration of medical care and nursing services, it is necessary to establish a long-term care insurance system, promote qualified old-age care institutions to become designated medical institutions, form a training and incentive mechanism for medical staff, and promote the development of internet medical care. To prevent the psychological risk of the elderly, it is necessary to pay attention to the psychological work to deal with the psychological crisis of the elderly. In addition, refined management is needed to avoid cross-infection.

7.
Article in Chinese | WPRIM | ID: wpr-846313

ABSTRACT

At present, anti-tumor chemotherapy drugs used in clinic commonly have serious side effects on normal tissues. Although targeted and antibody anti-tumor drugs have made progress in clinic, the high medical cost makes it difficult for many patients to afford. To find effective and low toxic anti-tumor agents without increasing medical cost, it is still the challenges of current research. Based on the natural products with selective cytotoxicity, we aimed to summarize the research progress, provide ideas for the basic research and clinical development of antitumor agents.

8.
Article in Chinese | WPRIM | ID: wpr-775083

ABSTRACT

OBJECTIVE@#To study the relationship between serum microRNA-122 (miR-122) and insulin resistance in obese children.@*METHODS@#Forty-seven children with severely obesity aged 7-14 years and 45 age- and gender matched healthy children with normal weight (control group) were enrolled. The levels of height, weight, waistline, hip circumference, fasting blood glucose (FBG), fasting insulin (FINS), triglyceride (TG), total cholesterol (TC), free fatty acid (FFA), interleukin-6 (IL-6) and miR-122 in the two groups were measured. Body mass index (BMI), waist-hip ratio (WHR) and insulin resistance index (HOMA-IR) were calculated.@*RESULTS@#Compared with the control group, the height, weight, BMI, WHR, FINS, HOMA-IR, TG, FFA, IL-6, and miR-122 levels in the obese group were significantly increased (P<0.05). MiR-122 levels in the obese group were positively correlated with FINS, HOMA-IR and IL-6 levels (r=0.408, 0.442, and 0.464 respectively, P<0.05). The changes of miR-122 have a linear regression relationship with IL-6 (b'=0.318, P<0.05).@*CONCLUSIONS@#The elevated serum miR-122 levels may be correlated with insulin resistance in obese children. The mechanism needs to be further studied.


Subject(s)
Adolescent , Blood Glucose , Body Mass Index , Child , Humans , Insulin , Insulin Resistance , MicroRNAs , Genetics , Obesity , Waist-Hip Ratio
9.
Article in Chinese | WPRIM | ID: wpr-691475

ABSTRACT

Objective: To evaluate the accuracy of virtual occlusal definition in non-Angle class I molar relationship, and to evaluate the clinical feasibility. Methods: Twenty pairs of models of orthognathic patients were included in this study. The inclusion criteria were: (1) finished with pre-surgical orthodontic treatment and (2) stable final occlusion. The exclusion criteria were: (1) existence of distorted teeth,(2) needs for segmentation,(3) defect of dentition except for orthodontic extraction ones,and (4) existence of tooth space. The tooth-extracted test group included 10 models with two premolars extracted during preoperative orthodontic treatment. Their molar relationships were not Angle class I relationship. The non-tooth-extracted test group included another 10 models without teeth extracted, therefore their molar relationships were Angle class I. To define the final occlusion in virtual environment, two steps were included: (1) The morphology data of upper and lower dentition were digitalized by surface scanner (Smart Optics/Activity 102; Model-Tray GmbH, Hamburg, Germany); (2) the virtual relationships were defined using 3Shape software. The control standard of final occlusion was manually defined using gypsum models and then digitalized by surface scanner. The final occlusion of test group and control standard were overlapped according to lower dentition morphology. Errors were evaluated by calculating the distance between the corresponding reference points of testing group and control standard locations. Results: The overall errors for upper dentition between test group and control standard location were (0.51 ±0. 18) mm in non-tooth-extracted test group and (0. 60 ±0. 36) mm in tooth-extracted test group. The errors were significantly different between these two test groups (P< 0. 05). However, in both test groups,the errors of each tooth in a single dentition does not differ from one another. There was no significant difference between errors in tooth-extracted test group and 1 mm (P>0.05); and the accuracy of non-tooth-extracted group was significantly smaller than 1 mm (P <0.05). Conclusion: The error of virtual occlusal definition of none class I molar relationship is higher than that of class I relationship, with an accuracy of 1 mm. However, its accuracy is still feasible for clinical application.

10.
Article in Chinese | WPRIM | ID: wpr-699612

ABSTRACT

Objective To observe the effects of Qijingmingmu decoction granule on the expression of mitogen-activated protein linase (MAPK) signal pathway in fibroblasts of conjunctivochalasis (CCH) under the stimulation of tumor necrosis factor (TNF-α) and to explore the pathogenesis and effective treatment method of CCH.Methods CCH conjunctival fibroblasts were cultured in vitro and divided into CCH group,CCH + TNF-α group and CCH + TNF-α + Qijingmingmu decoction group.CCK-8 assay was used to determine the effective concentration of Qijingmingmu decoction granule,and 10-2 mg · L-1 TNF-α was added in the cultured fibroblasts of the latter two groups,followed by interference with Qijingmingmu decoction granule for 48h.The expression of MAPK signal pathway related protein and mRNA were detected by ELISA,Western Blot and RT-PCR.Then the results were statistically analyzed.Results CCK-8 assay showed the effective concentration of Qijingmingmu decoction granule was 1.61g · L-1.And there were significant differences in the total A values (A450) of extracellular regulated protein kinase (ERK),c-jun N-terminal kinase (JNK),p38 mitogen activated protein kinase (p38 MAPK) and their phosphorylation levels among the three groups (all P < 0.05).Moreover,TNF-α could significantly up-regulate the expression of ERK1/2,JNK1/2,p-JNK1/2,p38 MAPK and p-p38 MAPK in CCH fibroblasts (all P <0.05),while Qijingmingmu decoction down-regulated their expressions in CCK fibroblasts after TNF-α stimulation (all P < 0.05).Furthermore,the total differences in p-ERK1/2,p38 MAPK and p-p38 MAPK protein were significant in the three groups (all P<0.05);and TNF-α could significantly up-regulate the expression of p-ERK1/2,p-JNK1/2,p38 MAPK and p-p38 MAPK protein in CCH fibroblasts (all P <0.05),while Qijingmingmu decoction down-regulated p-ERK1/2 and p38 MAPK expressions in CCK fibroblasts after TNF-α stimulation (both P < 0.05).In addition,the total differences in ERK1/2 and p38 MAPK mRNA were significant in the three groups (both P < 0.05);and TNF-α could significantly up-regulate the expression of ERK1/2 and p38 MAPK mRNA in CCH fibroblasts (both P < 0.05),while Qijingmingmu decoction down-regulated p38 MAPK mRNA expressions in CCK fibroblasts after TNF-α stimulation (P < 0.05).Conclusion The inflammatory factor TNF-α can up-regulate the expressions of MAPK signal pathway related protein and mRNA in CCH fibroblasts,resulting in the occurrence and development of CCH,and meanwhile Qijingmingmu decoction granule can downregulate the expression of MAPK signal pathway to play the therapeutic role in CCH to some extent.

11.
Article in Chinese | WPRIM | ID: wpr-311544

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression of death-associated protein kinase 1(DAPK1) in chronic lymphocytic leukemia(CLL).</p><p><b>METHODS</b>The DAPK1 expression was studied by means of MEC1 cells and B lymphocytes from blood samples of the patients with CLL. The quantitative detection of mRNA and Western blot were used to detecte the expression of DAPK1 and autophagy-related genes at both mRNA and protein levels.</p><p><b>RESULTS</b>mRNA quantitative detection and Western blot displayed that the DAPK1 expression in the patients with CLL was silenced. So, the expression of DAPK1 and autophagy related genes in MEC1 cells was not significantly different, no matter the cells were treated with or without INF-γ.</p><p><b>CONCLUSION</b>Scilencing of DAPK1 expression in CLL results in abnormality of autophagy behavior, thus leading to the occurence of disease.</p>

12.
Article in Chinese | WPRIM | ID: wpr-691449

ABSTRACT

The maxilla is the most important bony support of the mid-face skeleton and is critical for both esthetics and function.Maxillary defects,resulting from tumor resection,can cause severe functional and cosmetic deformities.Furthermore,maxillary reconstruction presents a great challenge for oral and maxillofacial surgeons.Nowadays,vascularized composite bone flap transfer has been widely used for functional maxillary reconstruction.In the last decade,we have performed a comprehensive research on functional maxillary reconstruction with free fibula flap and reported excellent functional and acceptable esthetic results.However,this experience based clinical procedure still remainssome problems in accuracy and efficiency.In recent years,computer assisted techniques are now widely used in oral and maxillofacial surgery.We have performed a series of study on maxillary reconstruction with computer assisted techniques.The computer assisted techniques used for maxillary reconstruction mainly include:(1) Three dimensional (3 D) reconstruction and tumor mapping:providing a 3 D view of maxillary tumor and adjacent structures and helping to make the diagnosis of maxillary tumor accurate and objective;(2) Virtual planning:simulating tumor resection and maxillectomy as well as fibula reconstruction on the computer,so that to make an ideal surgical plan;(3) 3D printing:producing a 3D stereo model for prebending individualized titanium mesh and also providing template or cutting guide for the surgery;(4) Surgical navigation:the bridge between virtual plan and real surgery,confirming the virtual plan during the surgery and guarantee the accuracy;(5) Computer assisted analyzing and evaluating:making a quantitative and objective of the final result and evaluating the outcome.We also performed a series of studies to evaluate the application of computer assisted techniques used for maxillary reconstruction,including:(1)3D tumor mapping technique for accurate diagnosis and treatment of maxillary tumor;(2) Maxillary reconstruction with free fibula flap used computer assisted techniques;(3) Computer assisted orbital floor reconstruction after maxillectomy.The results suggested that computer assisted techniques could significantly improve the clinical outcome of maxillary reconstruction.

13.
International Eye Science ; (12): 1883-1885, 2017.
Article in Chinese | WPRIM | ID: wpr-641084

ABSTRACT

AIM: To investigate the curative effect of phacoemulsification and intraocular lens ( IOL ) implantation combined with goniosynechialysis in the treatment of age-related cataract merging with primary angle-closure glaucoma ( PACG) . · METHODS: Totally 80 patients with age-related cataract merging with PACG were in our hospital from January 2014 to January 2016. The preoperative average intraocular pressure ( IOP) was 33. 22 ± 3. 17mmHg; the average depth of anterior chamber was 2. 07 ± 0. 15mm;the dynamic situation of primary angle closure ≤1/2 cycle by gonioscope. They were randomly divided into Group A and B for doing a study. All the two groups were treated with phacoemulsification and intraocular lens implantation. And the Group A was with goniosynechialysis. The following up period was 2mo, and we observed the IOP, chamber depth and the anterior chamber angle. · RESULTS: The change of chamber depth and intraocular pressure about the two groups: the average intraocular pressure of the Group A was 15. 11 ± 3. 67mmHg,the chamber depth was 3. 11±0. 08mm;those of the Group B were 17. 24 ± 1. 67mmHg, 2. 76 ± 0. 15mm respectively; the differences had statistical significance (P<0. 05). Postoperatively, there were 28 eyes (70%) in Group A with fully open anterior chamber angle, and 18 eyes (45%) in Group B (P<0. 05). · CONCLUSION: The phacoemulsification and intraocular lens implantation combined with goniosynechialysis in the treatment of age-related cataract merging with primary angle-dosure glaucoma is safe and reliable. It's simple to operate, and do not increase the risk of surgery.

14.
Chinese Medical Journal ; (24): 696-701, 2016.
Article in English | WPRIM | ID: wpr-328172

ABSTRACT

<p><b>BACKGROUND</b>Antimicrobial peptides, including cathelicidin LL-37, human beta defensin (HBD)-2, and HBD-3, are important elements of the innate immune response and involved in modulation of the adaptive immunity, and they also play an important role in cutaneous defense against Mycobacterium tuberculosis.</p><p><b>METHODS</b>The fresh skin tissues and paraffin-embedded biopsy samples from three cutaneous tuberculosis, two tuberculids, and ten healthy individuals were collected. The expressions of LL-37, HBD-2, and HBD-3 mRNA in the lesions of three cutaneous tuberculosis and two tuberculids were detected by quantitative real-time polymerase chain reaction; the protein expressions were detected by immunohistochemistry and Western blotting methods.</p><p><b>RESULTS</b>The expressions of LL-37 mRNA and protein in the lesions of cutaneous tuberculosis and tuberculids were similar to that of normal skin. The expression of HBD-2 mRNA had an increasing trend in the lesions of cutaneous tuberculosis and tuberculids compared with that of normal skin; however, the expression of HBD-2 protein in the lesions of cutaneous tuberculosis had a decreasing trend compared with that of normal skin, and the expression of HBD-2 protein in the lesions of tuberculids was similar to that of normal skin. The expressions of HBD-3 mRNA and protein in lesions of cutaneous tuberculosis and tuberculids were similar to that of normal skin.</p><p><b>CONCLUSIONS</b>Our study indicated that the expression of HBD-2 and HBD-3 mRNA and protein in lesions of cutaneous tuberculosis may be not consistent with that of tuberculids. However, an inherent limitation of the present study was that the sample size was small, and the roles and regulation mechanisms of LL-37, HBD-2, and HBD-3 in cutaneous tuberculosis and tuberculids need to be further investigated.</p>


Subject(s)
Adult , Aged , Antimicrobial Cationic Peptides , Genetics , Female , Humans , Male , Middle Aged , RNA, Messenger , Tuberculosis, Cutaneous , Metabolism , beta-Defensins , Genetics
15.
Chinese Medical Journal ; (24): 1483-1490, 2016.
Article in English | WPRIM | ID: wpr-290049

ABSTRACT

<p><b>BACKGROUND</b>Tetracycline (TET) has been found to have both antibiotic and anti-inflammatory properties. The anti-inflammatory effect of topical TET on atopic dermatitis (AD) has not been reported. The purpose of this study was to explore the potential role of topical TET and its anti-inflammatory effects in a mouse model of AD.</p><p><b>METHODS</b>The 2% TET was applied topically to ears of MC903-induced AD-like BALB/c mice once a day. AD-like symptoms and severity were evaluated by assessing skin scoring of dermatitis, ear thickness, and frequency of scratching. Serum IgE and thymic stromal lymphopoietin (TSLP) levels were measured by enzyme-linked immunosorbent assay. Western blot was used for analyzing the expressions of TSLP, protease-activated receptor 2 (PAR2), and nuclear factor-kappa B (NF-κB) in skin lesions. Real-time polymerase chain reaction was performed to assess the mRNA levels of TSLP and inflammatory cytokines including interleukin (IL)-4, IL-13, tumor necrosis factor (TNF)-α, and IL-1β in skin lesions.</p><p><b>RESULTS</b>Scoring of dermatitis (9.00 ± 0.63 vs. 6.67 ± 1.03, P = 0.001), ear thickness (0.44 ± 0.02 mm vs. 0.40 ± 0.03 mm, P = 0.018), and serum IgE level (421.06 ± 212.13 pg/ml vs. 244.15 ± 121.39 pg/ml, P = 0.047) were all improved in the 2% TET treatment group compared with AD group. Topical TET significantly reduced the serum level of TSLP (119.04 ± 38.92 pg/ml vs. 65.95 ± 54.61 pg/ml, P = 0.011) and both mRNA and protein expressions of TSLP in skin lesions compared with AD group (P = 0.003 and 0.011, respectively), and NF-κB and PAR2 expression in skin lesions were also suppressed (P = 0.016 and 0.040, respectively). Furthermore, expressions of inflammatory cytokines IL-4, IL-13, and TNF-α in skin lesions were down-regulated in 2% TET group compared with AD group (P = 0.035, 0.008, and 0.044, respectively).</p><p><b>CONCLUSIONS</b>Topical TET exerted anti-inflammatory effects through suppression of TSLP and inflammatory cytokines in AD mouse model, suggesting TET as a potential agent for the topical treatment of AD in the future.</p>


Subject(s)
Administration, Topical , Animals , Calcitriol , Toxicity , Cytokines , Dermatitis, Atopic , Drug Therapy , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Female , Interleukin-13 , Metabolism , Interleukin-4 , Metabolism , Mice , Mice, Inbred BALB C , Tetracyclines , Therapeutic Uses , Tumor Necrosis Factor-alpha , Metabolism
16.
Chinese Journal of Stomatology ; (12): 645-647, 2013.
Article in Chinese | WPRIM | ID: wpr-274166

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the application of computer-aided design and navigation technology in skull base and infratemporal fossa tumor surgery and to analyze its advantages and disadvantages.</p><p><b>METHODS</b>Twenty-nine cases with tumor of skull base and infratemporal fossa were treated with computer-aided design and navigation surgery. The Parameters of age, gender, primary or recurrent tumor, tumor nature and surgical approach were recorded.</p><p><b>RESULTS</b>En bloc resection was performed in 20 cases and subtotal resection in 9 cases. The margin status was negative margin in 8 cases, near-tumor margin in 17 cases and positive resection margin in 4 cases. Postoperative complication rate was 14% (4/29). During the follow-up period, 2 benign cases recurred.In the malignant group, there were 7 cases of recurrence, 2 cases of metastasis and 3 deaths. The 5-year overall survival and progression-free survival rate were 69% and 40% respectively.</p><p><b>CONCLUSIONS</b>Navigation technology can enhance the confidence of the surgeons and operation safety in handling malignant tumors in skull base and infratemporal fossa.</p>


Subject(s)
Adolescent , Adult , Aged , Child , Child, Preschool , Computer-Aided Design , Cranial Fossa, Middle , Diagnostic Imaging , Pathology , General Surgery , Female , Follow-Up Studies , Humans , Imaging, Three-Dimensional , Male , Middle Aged , Neoplasm Recurrence, Local , Postoperative Complications , Skull Base , Diagnostic Imaging , Pathology , General Surgery , Skull Base Neoplasms , Diagnostic Imaging , Pathology , General Surgery , Tomography, X-Ray Computed , Treatment Outcome , Young Adult
17.
Chinese Journal of Stomatology ; (12): 359-362, 2013.
Article in Chinese | WPRIM | ID: wpr-293589

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the characteristic of the maxillary molars with two palatal roots and its prevalence by cone-beam computered tomography.</p><p><b>METHODS</b>Cone-beam CT (CBCT) images of dentitions were collected retrospectively from 528 patients comprising 1957 maxillary molars. The characteristic and prevalence of maxillary molars with two palatal roots was analyzed. The tooth position, sex and unilateral and bilateral occurrence were recorded.</p><p><b>RESULTS</b>The prevalence of two palatal roots was 0.72%(14/1957). Maxillary second molar with two palatal roots was detected in 1.12%(11/979), which showed a statistically higher prevalence than maxillary first molar 0.31%(3/978). This prevalence did not differ with sex or the side(P > 0.05). The mean distance of interorifice from the mesiolpalatal canal to the distopalatal canal was (2.84 ± 0.50) mm, and the angle between two palatal roots was on average (34.6 ± 16.1) °,which were significantly greater than the mean distance of interorifice from the two buccal roots (2.15 ± 0.82) mm and the angle between the two roots was (21.3 ± 12.2) °.</p><p><b>CONCLUSIONS</b>The prevalence of maxillary second molars with two palatal roots was higher than that of the first malars. The angle between the two palatal roots brings difficulty to root canal therapy, so the consciousness of double palatal roots of maxillary molars was helpful for clinical operation.</p>


Subject(s)
Adolescent , Adult , Aged , Cone-Beam Computed Tomography , Methods , Dental Pulp Cavity , Diagnostic Imaging , Female , Humans , Male , Maxilla , Diagnostic Imaging , Middle Aged , Molar , Diagnostic Imaging , Prevalence , Retrospective Studies , Tooth Apex , Diagnostic Imaging , Tooth Root , Congenital Abnormalities , Diagnostic Imaging , Young Adult
18.
Chinese Journal of Oncology ; (12): 666-671, 2013.
Article in Chinese | WPRIM | ID: wpr-267479

ABSTRACT

<p><b>OBJECTIVE</b>To detect K-ras gene mutations in plasma free DNA by peptide nucleic acid clamp PCR assay (PNA-PCR) and nested primer PCR, and to analyze the correlation between K-ras mutations and prognosis in patients with metastatic colorectal cancer (mCRC).</p><p><b>METHODS</b>Peripheral blood was collected and free DNA was extracted from plasma in 106 patients with mCRC. Nested primer PCR and PNA-PCR were used to detect K-ras gene mutation in the plasma free DNA. The patients were divided into three groups by K-ras status: wild-type group (wild-type determined by both methods), low mutation group (mutation by PNA-PCR method, wild-type by nested primer PCR method) and high mutation group (mutation by two methods). The correlation between K-ras mutations and prognosis was analyzed.</p><p><b>RESULTS</b>The mutation rate of K-ras in tumor tissues of the 106 patients was 40.6%. The Mutation rate of K-ras in plasma free DNA detected by PNA-PCR was 31.1%, significantly higher than that of 15.1% detected by nested primer PCR (P = 0.006). The consistent rate of the K-ras status in plasma free DNA detected by PNA-PCR and that in tumor tissue detected by traditional method was up to 83.0%. The median overall survival (OS) of patients of the wild type, low mutation and high mutation groups was 23.5 months, 17.3 months and 13.9 months, respectively (P = 0.002). The median progression-free survival (PFS) of the K-ras wild-type, low mutation and high mutation groups with first-line chemotherapy was 6.8 months, 6.1 months and 3.2 months, respectively (P = 0.002), and the median OS of them were 23.0 months, 15.5 months and 13.9 months, respectively (P = 0.036). The overall response rate (ORR) was improved in the K-ras wide-type patients who received cetuximab combined with chemotherapy as first-line therapy (75.0% vs. 23.4%, P = 0.058). Cetuximab combined with in second-line therapy chemotherapy led to a significant improvement in disease control rate (DCR) ( 100% vs. 35.7%, P < 0.001) as compared with those of chemotherapy alone. COX regression model showed that K-ras status detected by PNA-PCR, ECOG PS, number of surgery and initially metastatic site were independent factors for prognosis.</p><p><b>CONCLUSIONS</b>PNA-PCR for the detection of K-ras mutation in plasma free DNA can be used to substitute the traditional method for detection of K-ras mutation in tumor tissues. The abundance of K-ras mutation in plasma free DNA is an independent prognostic factor for patients with metastatic colorectal cancer.</p>


Subject(s)
Adult , Aged , Antibodies, Monoclonal, Humanized , Therapeutic Uses , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Cetuximab , Colorectal Neoplasms , Genetics , Metabolism , Pathology , DNA , Blood , Disease-Free Survival , Female , Follow-Up Studies , Genes, ras , Humans , Liver Neoplasms , Lung Neoplasms , Male , Middle Aged , Mutation , Peptide Nucleic Acids , Polymerase Chain Reaction , Remission Induction , Retrospective Studies , Survival Rate , Young Adult , ras Proteins , Genetics , Metabolism
19.
Article in Chinese | WPRIM | ID: wpr-253928

ABSTRACT

<p><b>OBJECTIVE</b>To explore an optimum method on postoperative recovery of general-anesthesia gynecological laparoscopic surgery.</p><p><b>METHODS</b>One hundred and twenty cases of gynecological laparoscopic surgery were randomly divided into three groups, 40 cases in each one. The sensitive points of bilateral Shenmen (TF4), Zigong (TF2), Wei (CO4), Dachang (CO7) and so on were selected in all three groups one night before the surgery. The sticking with magnetic beads was applied in the group A. The magnetic beads were in-pair stuck at front-back corresponding location of both ears in the group B. The same-appearance plaster was put at the corresponding acupoints in the group C. The intubation anesthesia was applied in all three groups and postoperative recovery of gynecological laparoscopic surgery was observed.</p><p><b>RESULTS</b>The postoperative visual analogue scale (VAS) was (1.77 +/- 1.65) in the group A and (1.80 +/- 1.96) in the group B, which was both lower than (2.62 +/- 1.46) in the group C (both P < 0.01). The occurrence rate of nausea and vomiting was 25.0% (9/36) in the group A and 20.0% (8/40) in the group B, which was both lower than 50.0% (19/38) in the group C (both P < 0.01). The recovery of borborygmus on postoperative 1st and 3rd day in the group A and B was faster than that in the group C (both P < 0.05), while time of fart and defecation in the group A and B was earlier than that in the group C (both P < 0.05). The score of state-trait anxiety inventory in the group A and B was lower than that in the group C (both P < 0.05), but the differences of each item between the group A and B were not obvious (both P > 0.05).</p><p><b>CONCLUSION</b>The auricular point sticking could support analgesia of general-anesthesia gynecological laparoscopic surgery, which could relieve anxiety mood, reduce occurrence of nausea and vomiting and improve function of stomach and intestine to benefit postoperative recovery. However, the effect of in-pair sticking of auricular point with magnetic beads at front-back acupoints was not obviously strengthened.</p>


Subject(s)
Acupuncture, Ear , Adult , Aged , Female , Humans , Laparoscopy , Middle Aged , Postoperative Nausea and Vomiting , Therapeutics , Young Adult
20.
Chinese Journal of Stomatology ; (12): 414-418, 2012.
Article in Chinese | WPRIM | ID: wpr-281597

ABSTRACT

<p><b>OBJECTIVE</b>To compare the efficacy of computer-navigated surgery and the 3-D skull models and guide plates for the treatment of unilateral delayed zygomatic fractures.</p><p><b>METHODS</b>Eleven patients with unilateral delayed zygomatic fractures were treated by computer-navigated surgery (test group) and another 12 patients were treated by 3-D skull models and guide plates as the control group. Quality of reduction was assessed by examination of postoperative axial CT scans through zygomatic arch, the malar prominence and the width of zygomatic arch were measured in both groups.</p><p><b>RESULTS</b>The difference between bilateral malar prominence was (0.94 ± 0.73) mm in the test group and (1.88 ± 1.82) mm in the control group, there was no significant difference between the two groups (P > 0.05). The difference between bilateral width of zygomatic arch was (0.77 ± 0.51) mm in the test group, less than (3.00 ± 1.81) mm in the control group (P < 0.05). Excessive malar prominence was observed in 6 cases (6/11) in the test group and in 7 cases (7/12) in the control group, whereas malar depression was observed in 5 cases (5/11) in the test group and in 5 cases (5/12) in the control group. Overcorrection of the width of zygomatic arch was observed in 6 cases (6/11) in the test group and in 12 cases (12/12) in the control group, whereas under correction was observed in 5 cases (5/11) in the test group and in 0 cases (0/12) in the control group.</p><p><b>CONCLUSIONS</b>In the treatment of unilateral delayed zygomatic fractures, symmetry of malar prominence can be achieved by application of computer-navigated surgery or the 3-D skull models and guide plates. Symmetry of the width of zygomatic arch achieved with the computer-navigated surgery is better than that achieved with 3-D skull models and guide plates.</p>


Subject(s)
Adult , Female , Fracture Fixation, Internal , Humans , Male , Middle Aged , Surgery, Computer-Assisted , Tomography, X-Ray Computed , Young Adult , Zygoma , Diagnostic Imaging , General Surgery , Zygomatic Fractures , Diagnostic Imaging , General Surgery
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