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Article in Chinese | WPRIM | ID: wpr-878954


The color characteristic information of Rhei Radix et Rhizoma powder was obtained by spectrophotometer, the feasibility of rapid identification of Rhei Radix et Rhizoma origin based on chromaticity value was studied by statistical analysis. The results of rank correlation analysis showed that a~*(P<0.01), b~*(P<0.01) had significantly correlation with the origin of medicinal herbs, which could be used as two important parameters to distinguish the origin of Rhei Radix et Rhizoma, the larger the a~* value, the more red the powder color,and the greater the b~* value, the more yellow the powder color. Meanwhile, through Fisher discriminant analysis, the linear discriminant functions of different genus Rhei Radix et Rhizoma were established, which was Rheum tanguticum=40.666a~*+0.019b~*-213.303, Rh. palmatum=34.121a~*+0.061b~*-151.770, Rh. officinale=28.071a~*+0.113b~*-104.604 3, the coincidence rate of cross-validation was over 95%, among them, the discriminant rate of Rh. tanguticum and Rh. officinale reached 100%;In addition, using the percentile method to analyze the 90% reference value range of three different origin of Rhei Radix et Rhizoma, as a result, Rh. tanguticum a~*(10.236 5-10.604 7), b~*(32.294 8-34.841 7); Rh. palmatum a~*(8.602 7-8.770 0), b~*(27.534 8-28.968 6), and Rh. officinale a~*(6.825 7-7.464 3),b~*(21.001 6-27.716 4). According to this study, rank correlation analysis and Fisher discriminant analysis are feasible to distinguish the base of Rhei Radix et Rhizoma in a certain range, and provide some theoretical basis for the identification of Rhei Radix et Rhizoma. It also provides a new method and idea for the identification of other multi-base Chinese medicine.

Animals , Drugs, Chinese Herbal , Gastropoda , Plant Roots , Rheum , Rhizome
Article in Chinese | WPRIM | ID: wpr-828431


In this study, HPLC was used to determine the content of the four isoflavones of Astragalus membranceus var. mongholicus from different regions(calycosin-7-glucoside, ononin, calycosin and formononetin), and gray correlation analysis and path analysis were used to explore the influence of climate factors on the content of isoflavone components in A. membranceus var. mongholicus. The results showed that there were significant differences in the content of the four isoflavones in different areas(P<0.05); grey correlation analysis showed that the highest temperature in July, the lowest temperature in January and the daily average temperature had a greater impact on the content of flavonoid glycosides, meanwhile precipitation and relative humidity were the more important factors for the accumulation of flavonoid aglycones. According to the general analysis, the direct positive effects of the lowest temperature in January and altitude on the contents of four isoflavones in A. membranceus var. mongholicus were significant. High altitude and extreme temperature conditions might be more adverse to the formation and accumulation of isoflavone components. Therefore, the religions of A. membranceus var. mongholicus with high contents of isoflavones should be chosen the low altitude region with higher minimum temperature in January. This study provides a reference basis for the quality evaluation of A. membranceus var. mongholicus, and basic data for the selection of suitable habitat, construction of planting standards and directional cultivation of medicinal materials in A. membranceus var. mongholicus.

Astragalus Plant , Astragalus propinquus , Chromatography, High Pressure Liquid , Isoflavones , Plant Roots , Chemistry
Journal of Preventive Medicine ; (12): 462-465, 2015.
Article in Chinese | WPRIM | ID: wpr-792406


Objective To understand epidemiological characteristics of self -reported acute gastroenteritis in Hangzhou -Jiaxing -Huzhou area in Zhejiang Province.Methods According to the population capacity,the household interview was conducted among families selected by multi stage sampling method from July 2010 to June 2011,and one person who was approaching birthday in every family was selected for investigation, including symptoms and treatment of acute gastroenteritis.Results Totally 9 548 people were investigated.The monthly prevalence of acute gastroenteritis among the surveyed population was 2.95% and the incidence was 0.39 per person year.It was estimated that there would be 5.875 6 million cases of acute gastroenteritis occurred during this year in the area.Logistic regression analysis showed that monthly prevalence in female was higher than in male.The monthly prevalence reached the high level in July and August.The monthly prevalence in preschool children was the highest.The monthly prevalence in rural population was higher than that in the urban population.Besides,the monthly prevalence in those of family number ≥3 was higher than that of family number less than 3.Totally,56.38% of the cases visited docter,and 54.67% of the cases took antibiotics;13.48%reported work absence and 2.13% reported school absence due to the illness.Conclusion The disease burden of acute gastroenteritis could be heavy in Hangzhou -Jiaxing -Huzhou area.Gender,education,season,residence and the size of family may have some effects on the occurrence of acute gastroenteritis.

Article in Chinese | WPRIM | ID: wpr-313551


<p><b>OBJECTIVE</b>to observe the expression of the connective tissue growth (CTGF) and a smooth muscle actin (α-SMA) in acute paraquat (PQ) poisoned rats and investigate the mechanism of paraquat-induced pulmonary fibrosis.</p><p><b>METHODS</b>sprague-Dawley rats were randomly divided into two experimental groups: control group (6 rats) and PQ group (56 rats). On the 3rd, the 7th, the 14th, the 28th and the 56th day after exposure, the expression of CTGF and α-SMA were evaluated by SABC Immunohistochemistry and Western blot; and the relationship of the expression with pathologic score, hydroxyproline were also analyzed, respectively. The lung pathological changes of rats were observed and pathological evaluation was made.</p><p><b>RESULTS</b>it was similar that the expression pattern of CTGF, α-SMA detected by immunohistochemistry and Western blot. With the time passing, their expression in PQ group increased gradually compared with control group (P < 0.05 or P < 0.01). The increasing extent of CTGF, α-SMA were gentle on the 3rd, the 7th day. While their increasing extent was rapid from the 14th to the 56th day. CTGF was positively correlated with α-SMA, pathologic score and hydroxyproline respectively (r = 0.74, r = 0.87, r = 0.71, P < 0.05 or P < 0.01). Meanwhile, the histological changes such as lung fibroblast proliferation, disorganized collagen fibers were observed in PQ group.</p><p><b>CONCLUSION</b>CTGF and α-SMA could play an important role in the development of pulmonary fibrosis caused by paraquat poisoning; CTGF may promote the transformation of fibroblasts to myofibroblasts and further strengthen the ability of synthesis collagen and extracellular matrix.</p>

Actins , Metabolism , Animals , Connective Tissue Growth Factor , Metabolism , Male , Paraquat , Toxicity , Pulmonary Fibrosis , Metabolism , Pathology , Rats , Rats, Sprague-Dawley