Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add filters








Language
Year range
1.
Article in Chinese | WPRIM | ID: wpr-850816

ABSTRACT

Based on the concept of quality marker (Q-marker) and the application of Q-marker in traditional Chinese medicine, the quality control methods of Chinese materia medica injections were put forward, taking salvianolic acids for injection as an example. Through the analysis of components, efficacy and pharmacokinetics, it has been identified that the Q-marker of salvianolic acids for injection were salvianolic acid B, rosmarinic acid, lithospermic acid, salvianolic acid D, and salvianolic acid Y. The quality control system of multi-index content determination, fingerprint, near infrared on-line control, and biological quality control was established with Q-marker as the core. It provides a reference for the new ideas of quality control of Chinese materia medica injection.

2.
Article in Chinese | WPRIM | ID: wpr-774537

ABSTRACT

The research of anti-hepatocellular carcinoma(HCC) drug has attracted more and more attention. Natural products are the important source of active compounds for cancer treatment. A biflavonoid HIS-4 was isolated from Resina draconis in our previous study. MTT assay, hoechst staining, and flow cytometry analysis were used to investigate the effects of HIS-4 on the proliferation and apoptosis of human hepatoma HepG2 and SK-HEP-1 cells. Moreover, the effects of HIS-4 on the migration and invasion ability of HepG2 and SK-HEP-1 cells were evaluated by wound healing assay and Transwell assay. In addition, MTT assay, flow cytometry analyses, Hoechst staining, wound healing assay, Transwell assay, and tube formation assay were used to explore the anti-angiogenic activity of HIS-4 in human umbilical vein endothelial cells(HUVECs). Mechanistically, the HIS-4 regulatory of signal pathways in H9 epG2 and SK-HEP-1 cells were analyzed by Western blot. This results showed that HIS-4 suppressed the proliferation of human hepatoma HepG2 and SK-HEP-1 cells. Moreover HIS-4 induced their apoptosis of HepG2 and SK-HEP-1 cells. HIS-4 inhibited the migration and invasion of HepG2 and SK-HEP-1 cells. Additionally, HIS-4 exhibited angiogenesis effects. Mechanistically, up-regulation of MAPK signaling pathway and down-regulation of mTOR signaling pathway may be responsible for anti-hepatoma activity of HIS-4. Therefore, HIS-4 may be a promising candidate drug for HCC treatment.


Subject(s)
Antineoplastic Agents, Phytogenic , Pharmacology , Apoptosis , Biflavonoids , Pharmacology , Carcinoma, Hepatocellular , Drug Therapy , Pathology , Cell Movement , Cell Proliferation , Dracaena , Chemistry , Hep G2 Cells , Humans , Liver Neoplasms , Drug Therapy , Pathology , Phytochemicals , Pharmacology
3.
Article in Chinese | WPRIM | ID: wpr-854527

ABSTRACT

Objective: To study the phenolic constituents of Draconis Resina. Methods: The compounds were isolated and purified by silica gel and sephadex LH-20 column chromatography, and their structures were identified by physiochemical properties and MS and NMR spectroscopic data. Results: Fourteen compounds were isolated from the ethyl acetate extract of Draconis Resina and identified as 4'-hydroxy-1', 4″-dimethoxychalcane (1), 7-hydroxy-5, 4'-dimethoxy-2-arylbenzofuran (2), pterostilbene (3), 3, 4'-dihydroxy-5- methoxystilbene (4), 5, 7, 4'-trihydroxyflavanone (5), pinocembrin (6), 7-hydroxyflavanone (7), liquiritigenin (8), methyl 4- hydroxybenzoate (9), methyl 4-hydroxy-3-methoxybenzoate (10), ethyl 4-hydroxy-3-methoxybenzoate (11), 2, 4-dihydroxy acetophenone (12), 3, 4-dihydroxyallybenzene (13), and β-sitosterol (14). Conclusion: Compound 1 is a new natural product. Compound 2 is firstly obtained from the plants of Liliaceae. Compounds 5 and 9-12 are firstly isolated from the plants of Dracaena Vand. ex L., and compounds 6 and 7 are isolated from Dracaena cochinchinensis for the first time.

4.
Article in Chinese | WPRIM | ID: wpr-330302

ABSTRACT

A quantitative HPLC-DAD method was developed for simultaneous determination of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine in Solani Melongenae Radix from different cultivation regions in China The separation was performed on an Agilent Eclipse XDB C18 column (4.6 mm x 250 mm, 5 microm) at 30 degrees C with a gradient elution of methanol and 0.1% formic acid in water as mobile phase. The flow rate was set at 1.0 mL x min(-1) and the detection wavelength was 300 nm. The calibration curves of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine were linear over the ranges of 2.84-68.16, 3.10-74.40 mg x L(-1), and the average recoveries (n = 9) were 99.30% and 102.8%, respectively. The developed method was successfully applied for the analysis of sixteen samples from different cultivation regions in China, which indicated that the method is simple, rapid, accurate, and reliable for quality evaluation of Solani Melongenae Radix.


Subject(s)
Amides , China , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Plant Roots , Chemistry , Solanaceae , Chemistry , Classification
SELECTION OF CITATIONS
SEARCH DETAIL