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1.
Article in Chinese | WPRIM | ID: wpr-824956

ABSTRACT

Objective: To explore the mechanism of An-pressing manipulation in improving post-stroke muscle spasticity, by observing the changes of γ-aminobutyric acid (GABA) and glycine (Gly) in plasma and gray matter of L1-L3 spinal cord anterior horn in post-stroke rats with muscle spasticity after An-pressing manipulation intervention. Methods: Ten of 80 adult male Sprague-Dawley (SD) rats were randomly selected as the blank group, and the remaining 70 were used for modeling. The middle cerebral artery occlusion (MCAO) rat model was established by insertion suture occlusion method in the left external carotid artery. Thirty rats with a Longa neurological score of 2-3 points and a modified Ashworth spasticity scale score of 1-, 1+, or 2 were included in the experiment. Using the random number table method, the 30 successfully modeled rats were randomly divided into a model group, an An-pressing tendon group and an An-pressing muscle belly group. Two days after modeling, rats in the An-pressing tendon group and An-pressing muscle belly group received An-pressing manipulation on the tendon and belly of quadriceps femoris muscle respectively, with the pressure of (350±50) g and the frequency of 5 s/time, 15 min per session, once a day for 5 continuous days. After the 5th treatment, the tension of the rat quadriceps femoris muscle was evaluated using the modified Ashworth spasticity scale. The Gly levels in rat plasma and L1-L3 segments of spinal cord were determined by enzyme-linked immunosorbent assay (ELISA). The GABA levels in rat plasma and L1-L3 segments of spinal cord were measured by high performance liquid chromatography (HPLC). Results: The decrease in rat muscle tension scored by the modified Ashworth spasticity scale in the An-pressing tendon group was more significant than that in the An-pressing muscle belly group (P<0.01); the increases in Gly and GABA levels in the rat plasma and L1-L3 segments of spinal cord were more significant in the An-pressing tendon group than those in the An-pressing muscle belly group (all P<0.01). Conclusion: Based on the theory of 'anti-stretch reflex' of tendon organs, the use of An-pressing manipulation to induce the 'anti-stretch reflex' by stimulating the tendon organs can improve the muscle spasticity of rats, which is better than An-pressing the muscle belly. Increased levels of Gly and GABA in rat plasma and L1-L3 segments of spinalcord may be one mechanism of An-pressing manipulation to improve muscle spasticity by stimulating tendon organs.

2.
Article in Chinese | WPRIM | ID: wpr-774549

ABSTRACT

Based on the fact that glycyrrhizic acid can form micelles in aqueous solution and play a role in solubilization, the optimal compatibility ratio between puerarin and glycyrrhizic acid was screened to prepare puerarin-glycyrrhizic acid dispersible tablets and investigate the dissolution of puerarin. The particle size, Zate potential and puerarin dissolution were compared among the micellar solutions with mass ratio of 7∶1, 6∶1, 5∶1, 4∶1, 3∶1 and 2∶1(puerarin to glycyrrhizic acid), and it was found that when the mass ratio of puerarin and glycyrrhizic acid was 5∶1, the micelle showed smallest particle size, uniform distribution, and largest puerarin dissolution, so mass ratio of 5∶1 was determined as the optimal condition. The formulation of puerarin-glycyrrhizic acid dispersible tablets was optimized by single factor and orthogonal test: puerarin 100.0 mg, glycyrrhizin 20.0 mg, polyvinylpolypyrrolidone 24.0 mg as disintegrating agent, microcrystalline cellulose 135.0 mg as stuffing bulking agent, hydroxypropyl methyl cellulose 18.0 mg as adhesive agent, magnesium stearate 2.7 mg as lubricant, and tablet weight of 300.0 mg. High-performance liquid chromatography(HPLC) method was used to determine the content of puerarin in dispersible tablets. Puerarin showed a good linear relationship(r=0.999 8) in the range of 15.5-248 g·L~(-1), with high precision(RSD<2.0%) and good repeatability(RSD<2.0%), and the recovery rate was 101.1%, RSD 0.89%. There was no significant difference in the quantity of puerarin in different batches of puerarin-glycyrrhizic acid dispersible tablets. When the artificial gastric juice was used as the dissolution medium, the dissolution of puerarin in puerarin-glycyrrhizic acid dispersible tablets could reach over 85% within 15 min. When phosphate buffer(pH 6.8) was used as the dissolution medium, the dissolution of puerarin in the puerarin-glycyrrhizic acid dispersible tablets had a faster dissolution rate in vitro, 99.8% in 30 min. Therefore, puerarin-glycyrrhizic acid dispersible tablets could improve the dissolution of puerarin in vitro due to the solubilization effect of glycyrrhizic acid.


Subject(s)
Glycyrrhizic Acid , Chemistry , Isoflavones , Chemistry , Solubility , Tablets
3.
Article in Chinese | WPRIM | ID: wpr-756709

ABSTRACT

Objective:To explore the optimal thermal effect parameter combination of An-pressing manipulation based on the pressing force, time and frequency, and to compare the thermal effect differences between the rhythmic and the continuous An-pressing manipulations. Methods:Three levels of light, moderate and heavy pressing forces were determined according to the An-pressing forces of the clinical tuina physicians; the pressing time and frequency parameters were determined according to the literatures about An-pressing manipulation. The volunteers were stimulated by the homemade An-pressing manipulation stimulator on the right Xinshu (BL 15), and then the three-factor and three-level orthogonal tests were carried out according to the test sequence specified by the L9(34) orthogonal table, and the temperature before and after pressing was recorded by an infrared thermal imaging system to screen the best parameters for the thermal effect of the An-pressing manipulation, thus to determine the optimal pressing parameters. The optimal parameters were then used for both continuous and rhythmic An-pressing manipulations to stimulate the bilateral Xinshu (BL 15). The temperature changes after pressing and the duration of the thermal effect (temperature difference ≤0.5℃ on both sides) were recorded by the infrared thermal imaging system, to explore the differences in the thermal effects of different An-pressing manipulations. Results:Among the three factors of pressing force, time and frequency, the influences of different pressing forces on temperature were significantly different (F=32.843,P=0.030), and the influence of 2.5 kg pressing force was the most significant; the effects of different pressing time on temperature were significantly different (F=54.102,P=0.018), and the pressing time of 7.5 min was the most significant; the influences of different pressing frequencies on temperature were not statistically significant (F=2.181,P=0.314), though the influence of 10 times/min pressing frequency was the largest. The influences on temperature difference of the rhythmic and the continuous An-pressing manipulations were significantly different (P=0.031 on the left side andP=0.045 on the right side), but there was no statistical difference in the duration of the thermal effect (P=0.690). Conclusion:The An-pressing manipulation parameters that significantly affect the temperature difference are pressing force and time. The optimal combination of thermal effect parameters is pressing force of 2.5 kg, time of 7.5 min, and frequency of 10 times/min. The local thermal effect of the rhythmic An-pressing manipulation is significantly greater than of the continuous An-pressing manipulation.

4.
Journal of Medical Postgraduates ; (12): 780-784, 2019.
Article in Chinese | WPRIM | ID: wpr-818323

ABSTRACT

Circular RNA is a special kind of endogenous non-coding RNA which is widely existing in eukaryotic cells. More studies confirmed that most circRNAs were produced in the process of RNA shearing and played a vital role in the occurrence and development of many diseases. Though there was not much research on circRNAs related to orthopaedic diseases in recent years, study found that circRNAs played a great significant role in the pathogenesis, diagnosis, target therapy of degenerative diseases(osteoarthritis, lumbar degenerative disease), metabolic diseases(osteoporosis) and motor system tumor(osteosarcoma). This study summarizes the research progress of circRNAs in orthopaedic diseases.

5.
National Journal of Andrology ; (12): 437-443, 2019.
Article in Chinese | WPRIM | ID: wpr-816815

ABSTRACT

Objective@#To investigate the prevalence of ED in adult men with inflammatory bowel disease (IBD).@*METHODS@#We searched PubMed, Cochrane Library, EMbase, WanFang Database, and China National Knowledge Infrastructure (CNKI) Database for original articles relating the prevalence of ED among male IBD patients in the past 20 years, and performed meta-analysis on the identified studies.@*RESULTS@#A total of 9 eligible studies involving 734 cases of IBD were included, of which the pooled prevalence of ED was 34.4% (95% CI: 30.8-38.1). Subgroup analyses showed that the incidence rate of ED was significantly higher in the patients with Crohn's disease than in those with ulcerative colitis (36.3% [95% CI: 30.1-42.9] vs 26.8% [95% CI: 21.9-32.3], P < 0.05), and in those with active than in those with remissive IBD (47.6% [95% CI: 38.3-57.2] vs 26.4% [95% CI: 22.0-31.4], P < 0.05). The prevalence of ED was the lowest in the patients with ulcerative colitis after treated by ileal pouch-anal anastomosis (17.2% [95% CI: 11.5-24.9]).@*CONCLUSIONS@#IBD is commonly associated with ED in male patients, and the risk of ED is increased in active IBD and decreased in remissive IBD.

6.
Article in Chinese | WPRIM | ID: wpr-712691

ABSTRACT

Objective:To observe the influence of pressing force and time on the thermal effect of An-pressing manipulation.Methods:Eight healthy volunteers were recruited to receive An-pressing manipulation at Xinshu (BL 15) on the right side.The pressing force and time were both divided into five levels:the force described as extremely mild,mild,moderate,strong and extremely strong and time given by 2.5 min,5.0 min,7.5 min,10.0 min and 15.0 min.The real-time change in local acupoint temperature as well as the change during 1.0-15.0 min after the manipulation were observed.Results:Compared with the baseline data,the real-time changes in the temperature after An-pressing Xinshu (BL 15) on the right side with different levels of force (from mild to strong) were respectively (1.88t0.64) ℃,(2.05±0.68) ℃,(2.25±0.59) ℃,(2.35±0.61) ℃ and (2.32±0.69) ℃;the changes in 15.0 min after the manipulation were respectively (-0.11±0.11) ℃,(0.03±0.14) ℃,(0.59±0.58) ℃,(1.38±0.70) ℃ and (2.09±0.98) ℃.The real-time temperature changes after the manipulation for different durations (from short to long) were respectively (1.94±0.37) ℃,(2.33±0.29) ℃,(2.49±0.31) ℃,(2.51±0.39) ℃ and (2.41±0.55) ℃;the changes in 15.0 min after the manipulation were respectively (0.53±0.49) ℃,(0.33±0.30) ℃,(0.52±0.33) ℃,(0.55±0.38) ℃ and (0.76±0.36) ℃.Conclusion:The thermal effect presented an increasing tendency with the extension of pressing time,and the temperature reached the top at 7.5 min;the thermal effect showed an increasing tendency with the rise of pressing force,and the temperature reached the top upon a moderate level of force.The pressing time can produce a greater influence on the real-time temperature than the pressing force;the pressing force can produce a greater influence on maintaining the temperature than the pressing time.

7.
Article in Chinese | WPRIM | ID: wpr-712655

ABSTRACT

Objective:To observe the effect of An-pressing manipulation on biceps brachii with delayed onset muscle soreness (DOMS) in healthy male volunteers.Methods:A total of 30 male college student volunteers were randomly divided into a blank group,a model group and a treatment group,10 cases in each group.Subjects in the blank group did not receive any intervention;subjects in the model group received active weight-bearing eccentric exercise on the non-favored side of the upper limb to establish the models,while not receiving any treatment;subjects in the treatment group received both the same modeling and An-pressing manipulation treatment.The subjective rating of perceived exertion (RPE),subjective soreness sensation threshold and soreness grade were evaluated before modeling,immediately after modeling,and 24,48,72,96 and 120 h after modeling.Serum total antioxidant capacity (T-AOC) was measured before modeling,immediately after modeling,and 24,48 and 72 h after modeling.Serum creatine kinase MM isoenzyme (CK-MM) was measured before modeling and 24,48 and 72 h after modeling.Results:At 24,48,72 and 120 h after treatment,the soreness grades of the treatment group were lower than those of the model group (all P<0.05).The RPE scores of the treatment group were lower than those of the model group (all P<0.05) immediately after modeling,at 24,48,72,96 and 120 h after modeling.The subjective soreness sensation threshold of the treatment group was higher than that of the model group immediately after modeling,at 24,48,72 and 96 h after modeling (all P<0.05).Immediately after modeling,T-AOC value in the treatment group was higher than that in the model group and blank group (both P<0.05).CK-MM of the treatment group was lower than that of the model group at 48 h and 72 h after modeling (P<0.05).Conclusion:An-pressing manipulation shows a certain therapeutic effect on biceps brachii with DOMS by strengthening the body's antioxidant and anti-damage abilities,which can effectively reduce the pain and accelerate the recovery from fatigue damage.

8.
Chinese Medical Journal ; (24): 1817-1821, 2016.
Article in English | WPRIM | ID: wpr-251297

ABSTRACT

<p><b>BACKGROUND</b>Small incision refractive lenticule extraction (SMILE) is an effective laser procedure that treats myopia. This research was to describe a novel approach to treat corneal ulcer or perforation using the corneal lenticules obtained from SMILE and to evaluate the safety and effectiveness of tectonic keratoplasty with femtosecond laser intrastromal lenticule (TEKIL).</p><p><b>METHODS</b>A total of twenty patients (22 eyes) were monitored for at least 6 months and were assessed using slit lamp microscopy, optical coherence tomography, and best-corrected visual acuity (BCVA). Postoperative complications throughout the study period were recorded.</p><p><b>RESULTS</b>Corneal ulcer in 14 patients (16 eyes) and corneal perforation in six patients (6 eyes) were treated with TEKIL. The patients were ten females and ten males, with a mean age of 58.5 ± 16.3 years (range: 16-81 years). In this study, the most causes of corneal ulcer or perforation were immunologic causes (54.5%). After TEKIL procedure, global integrity was achieved in all cases. No immune rejection or perforation was detected. The mean BCVA improved from 0.17 ± 0.20 preoperatively to 0.27 ± 0.25 postoperatively at the final follow-up (t = 2.095, P = 0.052). The postoperative BCVA improved in 12 eyes (54.5%) and maintained in nine eyes (40.9%). Vision function successfully maintained in all eyes (100%). In three cases, corneal ulcers were treated by reoperation of TEKIL at 3 months after the initial surgery for the reason that the residual corneal thickness was <250 μm.</p><p><b>CONCLUSIONS</b>TEKIL seems to be an effective treatment for corneal ulcer and perforation in the condition of emergency and donor shortage.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Cornea , General Surgery , Corneal Perforation , General Surgery , Corneal Transplantation , Methods , Corneal Ulcer , General Surgery , Female , Humans , Male , Middle Aged , Postoperative Complications , Retrospective Studies , Tomography, Optical Coherence , Visual Acuity , Physiology , Young Adult
9.
Article in English | WPRIM | ID: wpr-812161

ABSTRACT

This study aimed to profile the chemical constituents of Zi-Shen pill (ZSP) and its metabolites in plasma, urine, and prostate tissue, after administration into rats. Based on the chromatographic retention behavior, fragmentation patterns of chemical components, published literatures, and literature databases, an UPLC-Q-TOF/MS (LC-TOF/MS) method was established to identify the components of ZSP and its metabolites in biological samples. A total of 101 compounds were identified and tentatively characterized from the ZSP, including alkaloids, xanthones, and timosaponins. Except for 33 prototype components, 22 metabolites were detected in the plasma, urine, and prostate, and mainly came from Phellodendri Amurensis Cortex and Anemarrhenae Rhizoma. It was found that glucuronidation and sulfation were the major metabolic processes of xanthones, while oxidation, demethylation, and glucuronidation were the major metabolic pathways of alkaloids. In summary, the present study provided important chemical information on the metabolism of ZSP, indicating that alkaloids might be able to be absorbed into the prostate. The results provided a basis for further studies of the mechanisms of action for ZSP.


Subject(s)
Alkaloids , Blood , Urine , Animals , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Metabolism , Male , Plasma , Chemistry , Rats , Tandem Mass Spectrometry , Urine , Chemistry , Xanthones , Blood , Urine
10.
Article in Chinese | WPRIM | ID: wpr-236369

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of catecholamine hormone on the blood and brain of heroin addicts.</p><p><b>METHODS</b>Rats were divided into three groups and treated with the glucose (control group), the heroin (im) (heroin group), and the combination of the intramuscular injection of reserpine and heroin (reserpine group). Changes in the levels of the dopamine (DA), cAMP, and cGMP were detected by the radioimmunoassay (RIA) method in the blood and brain tissue.</p><p><b>RESULTS</b>No significant withdrawal symptoms were observed in the reserpine group. Compared with the control and heroin groups, the blood cAMP levels were increased by 35.36% and 15.53% in the reserpine group, respectively; the cAMP levels in the midbrain ventral tegmental area (VTA), prefrontal cortex (PFC), and hippocampus (Hipp) were increased by 24.08% & 8.53%, 15.66% & 8.13%, and 21.95% & 8.40%, respectively. While compared to the control and heroin groups, the DA levels of the PFC, Hipp, striatum, and nucleus accumbens (NAc) were significantly reduced in the reserpine group, decreasing by 74.09% & 82.86%, 81.06% & 82.23%, 91.62% & 86.55% and 84.35% & 90.63%, respectively. The concentrations of cGMP of the brain tissues in the reserpine group were lower than those in the control group. In addition, the neural electrophysiological testing showed that the electroencephalogram (EEG), electrocardiogram (ECG), and muscle spindle discharge diagram of rats in both the reserpine and heroin groups were apparently changed.</p><p><b>CONCLUSION</b>Catecholamine hormone plays an important role in heroin addiction.</p>


Subject(s)
Animals , Brain , Metabolism , Catecholamines , Physiology , Cyclic AMP , Blood , Metabolism , Cyclic GMP , Blood , Metabolism , Dopamine , Blood , Metabolism , Heroin Dependence , Metabolism , Male , Rats , Rats, Wistar
11.
Article in Chinese | WPRIM | ID: wpr-253913

ABSTRACT

<p><b>OBJECTIVE</b>To explore the impacts of electroacupuncture on median nerve conduction velocity and morphology in patients of carpal tunnel syndrome (CTS) and evaluate the efficacy of electroacupuncture on CTS.</p><p><b>METHODS</b>Thirty cases of CTS were treated with electroacupuncture at Quchi (LI 11), Yangchi (TE 4), Shousanli (LI 10), Neiguan (PC 6) and Hegu (LI 4) on the affected side, combined with semiconductor laser irradiation at Shenmen (HT 7) and Yangxi (LI 5) on the affected side. The improvements in the symptoms and changes in nerve conduction velocity and ultrasound morphology were observed before and after treatment.</p><p><b>RESULT</b>The clinical total effective rate was 96.7% (29/30). The median nerve conduction velocity was accelerated apparently and the amplitude was increased after treatment as compared with those before treatment, indicating the statistically significant difference (all P<0.001). The distal motor latency was shortened apparently (P<0.001) and the motor amplitude had no apparent change (P>0.05). The proximal median nerve swelling was relieved apparently after treatment (P<0.05) and the effective rate was 36.7% (11/30). There was no apparent difference in the ratio of the vertical and horizontal diameters of carpal tunnel after treatment as compared with that before treatment (P>0.05).</p><p><b>CONCLUSION</b>Electroacupuncture presents the positive repair function to the median nerve in the patients of CTS. It can effectively alleviate inflammatory reaction and relieve ischemia and swelling of nerve fibers. And it cannot induce the changes in morphology in a short term.</p>


Subject(s)
Adult , Aged , Carpal Tunnel Syndrome , Therapeutics , Electroacupuncture , Female , Humans , Male , Median Nerve , Middle Aged , Treatment Outcome , Young Adult
12.
Article in English | WPRIM | ID: wpr-820034

ABSTRACT

OBJECTIVE@#To discuss the expression and significance of vascular endothelial growth factor (VEGF) and p53 expression in rat degenerated intervertebral disc (IVD) tissues.@*METHODS@#A total of 78 even-aged Sprague-Dawley (SD) rats (observation group) were chosen to establish a rat IVD degeneration model with anterior limbs and tail-removing method. Three months after modeling, rats were executed and degenerated IVD tissues were obtained for pathological biopsy so as to observe VEGF and p53 expression thereof. Meanwhile, IVD tissues from 8 healthy rats were gained for comparison.@*RESULTS@#Capillary infiltration was found in 62.8% of cases in observation group. Positive expression of VEGF and p53 was seen in 74.4% and 59.0% of cases respectively with a co-expression rate of 53.8%. VEGF and p53 expression in degenerated IVD tissues with capillary infiltration was higher than that in non-infiltration group (P<0.05).@*CONCLUSIONS@#A coordinate expression of VEGF and p53 in rat degenerated IVD tissues is demonstrated, indicating that they are both involved in neovascularization and infiltration, which accelerates IVD degeneration.


Subject(s)
Animals , Chi-Square Distribution , Immunohistochemistry , Intervertebral Disc , Metabolism , Pathology , Intervertebral Disc Degeneration , Metabolism , Pathology , Male , Rats , Rats, Sprague-Dawley , Tumor Suppressor Protein p53 , Metabolism , Vascular Endothelial Growth Factor A , Metabolism
13.
Acta Pharmaceutica Sinica ; (12): 641-646, 2010.
Article in Chinese | WPRIM | ID: wpr-354577

ABSTRACT

To identify the structure of three related substances in potassium sodium dehydroandrographolide succinate (PSDS), an HPLC preparation method was used to separate the impurities. These main impurities were identified using LC-ESI/TOFMS, LC-ESI/MSn, NMR, UV and IR. One of the main impurities was a hydrolyzed and oxidized product of PSDS, which has not been reported previouely. The other two impurities were hydrolyzed products of PSDS after losing different succinic acids. The results indicate that PSDS can be easily hydrolyzed and oxidized. It should be stored at cool and dry places.


Subject(s)
Andrographis , Chemistry , Antiviral Agents , Chemistry , Chromatography, High Pressure Liquid , Diterpenes , Chemistry , Drug Contamination , Drugs, Chinese Herbal , Chemistry , Molecular Structure , Plants, Medicinal , Chemistry , Spectrometry, Mass, Electrospray Ionization , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
14.
Article in Chinese | WPRIM | ID: wpr-313160

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the therapeutic mechanism of Weiweifang (WWF, a Chinese herbal preparation) on gastric ulcer in rats viewing from metabonomics.</p><p><b>METHODS</b>Wistar rats were made to gastric model by acetic acid cauterization and randomized into the model group, the spontaneously healing group and the three WWF treatment groups, and a group of normal rats was set for control. Metabolic spectra of gastric mucosa extraction of rats were acquired with gas chromatography-mass spectrometry (GC-MS) technique. After being pre-processing, data were subjected to partial least squares discriminant analysis (PLS-DA) to discover the biomarkers in rats of the normal group and the model group. The therapeutic effect of WWF on experimental gastric ulcer was assessed by principal component analyses (PCA), and its action of mechanism was explained viewing from the changes of biomarkers.</p><p><b>RESULTS</b>Spectra of biomarkers, including organic acids, fatty acids, amino acids, etc. in model rats were statistically different to those in normal rats, which demonstrated that the energy and substance metabolisms were disordered in rats with gastric ulcer. WWF could cure gastric ulcer effectively by way of regulating the metabolism of gastric mucosa.</p><p><b>CONCLUSION</b>The therapeutic mechanism of WWF on experimental gastric ulcer in rats is revealed integrally by metabonomics in this study, displaying prominently the characteristics of Chinese medicine multiple targets comprehensive therapy.</p>


Subject(s)
Acetic Acid , Amino Acids , Metabolism , Animals , Drugs, Chinese Herbal , Therapeutic Uses , Fatty Acids , Metabolism , Gas Chromatography-Mass Spectrometry , Gastric Mucosa , Metabolism , Male , Metabolomics , Methods , Phytotherapy , Rats , Rats, Wistar , Stomach Ulcer , Drug Therapy , Metabolism
15.
Article in Chinese | WPRIM | ID: wpr-328678

ABSTRACT

<p><b>OBJECTIVE</b>To investigate a modified midface lift for baggy deformity of low eyelid, zygomatic laxity, periorbital wrinkle and deep nasolabial groove.</p><p><b>METHODS</b>Through the incision at palpebral margin for baggy eyelid, the dissection was performed under the periosteum of midface. The orbicularis oculi and zygomatic major muscles were suspended to lift the midface through the incision at temporal area.</p><p><b>RESULTS</b>During the recent 5 years, 42 cases were treated with satisfactory results.</p><p><b>CONCLUSIONS</b>The modified midface lift is easily performed with low morbidity and good safety. It is one of the best choices for mini-invasive midface rejuvenation.</p>


Subject(s)
Adult , Eyelids , General Surgery , Facial Muscles , Female , Humans , Middle Aged , Rhytidoplasty , Methods , Skin Aging
16.
Chinese Medical Journal ; (24): 1583-1587, 2008.
Article in English | WPRIM | ID: wpr-293956

ABSTRACT

<p><b>BACKGROUND</b>Previous reports have confirmed that edaravone has protective effects against ischemia-reperfusion (IR) injury of many organs. In this study, we investigated the effect of edaravone on preventing IR injury of the lung in a canine lung transplantation model.</p><p><b>METHODS</b>Twelve weight-matched pairs of random-bred dogs were randomized into two groups. Within each pair, one dog served as donor and the other as recipient. In the study group, prostaglandin E1(PGE1)(8 microg/kg) was injected into the donor pulmonary artery (PA) before occlusion and the donor lungs were flushed with 1.0 L of LPD solution containing edaravone (10 mg/kg) and stored in the same LPD solution at a temperature of 1 degrees C for 8 hours. The left single lung transplantation was then performed and recipients received intravenous injection with edaravone (10 mg/kg) at the onset of reperfusion. In the control group, edaravone was substituted by the same volume of sterile saline solution. Another six dogs were obtained as normal control group in which left lungs were dissected after thoracotomy without an IR injury. One hour after reperfusion, or after dissection of the left lung, the right lung was excluded from perfusion and ventilation after which, cardiopulmonary parameters were measured. Wet/dry ratios, malondialdehyde (MDA) and myeloperoxidase (MPO) levels were assessed and histological analysis of lung tissue performed at the same time.</p><p><b>RESULTS</b>All animals survived until the end of the experiment. The study group showed significantly decreased wet/dry ratios (treated: (74.1 +/- 4.2)% vs control: (86.8 +/- 5.2)%, P < 0.01), MDA levels (treated: 0.50 +/- 0.08 vs. control: 0.88 +/- 0.15, P < 0.01) and MPO activity (treated: 0.23 +/- 0.05 vs. control: 0.43 +/- 0.07, P < 0.01) compared to the control group two hours after occlusion of the right side. In the control group, pulmonary vascular resistance (PVR) was increased markedly and arterial oxygen partial pressure deteriorated significantly after exclusion of the right side compared to those in the treatment group.</p><p><b>CONCLUSIONS</b>Edaravone attenuates IR-induced lung injury and preserves lung function by inhibiting oxidative stress and decreasing leukocyte extravasation in a canine lung transplantation model.</p>


Subject(s)
Animals , Antipyrine , Pharmacology , Therapeutic Uses , Disease Models, Animal , Dogs , Lipid Peroxidation , Lung , Metabolism , Pathology , Lung Transplantation , Nitric Oxide , Oxidative Stress , Peroxidase , Metabolism , Reperfusion Injury
17.
Article in Chinese | WPRIM | ID: wpr-229964

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the feasibility of using cultured corneal endothelial cell (CECs) transplantation for cornea endothelium cell destruction with Gelatin membrane as the carrier in rabbits.</p><p><b>METHODS</b>The cultured CECs were labeled by Brdu and subcultured in vitro on glutaraldehyde-fixed Gelatin membranes and then the membranes were glued by alpha-cyanoacrylate alkyl to 7.00 mm autologous rabbit corneal bottons whose endothelium were mechanically removed previously. The buttons were sutured in place. With this method the right eyes of 21 rabbits were transplanted with CECs, and the right eyes of another 17 rabbits were transplanted with non-cells carrier as controls. The rabbits were bred and observed by slit microscopy and confocal microscopy at 1, 2, 4, 8, and 12 weeks after surgeries. Also, introcular pressure and corneal thickness were measured by Perkin's tonometer and ultrasonic pachymeter. After 12 weeks, all the animals were sacrificed and the grafts were examined by light microscopy and electronic microscopy.</p><p><b>RESULTS</b>CECs grew well on the gelatine memberane, and formed confluent monolayers in 3-5 days; the cell density reached as high as 2700 cells/mm2. After 2 weeks of operation, all corneal buttons were edema and began to be opaque. The control eyes remained opaque throughout the observation period. In eyes with CECs transplanted, the grafts began to be clear and thin 4 weeks after operation. The cell density of grafts decreased along with time, and the mean cell density of CECs transplantation buttons was (2023.3 +/- 330.3) cells/mm2 12 weeks after operation. The transplanted cells were stained with the anti-Brdu monoclonal antibody.</p><p><b>CONCLUSION</b>It is feasible to culture and translate CECs with the Gelatin membrane.</p>


Subject(s)
Animals , Cells, Cultured , Corneal Endothelial Cell Loss , Pathology , Therapeutics , Endothelial Cells , Transplantation , Endothelium, Corneal , Cell Biology , Rabbits
18.
Article in Chinese | WPRIM | ID: wpr-813880

ABSTRACT

OBJECTIVE@#To observe the therapeutic effect of Bacillus acidi lactici on Helicobacter Pylori (Hp) infectious gastritis in Balb/c mouse model so as to explore a possible non-antibiotic treatment for Hp.@*METHODS@#To establish a Balb/c mouse model with Hp infectious gastritis through inoculation of mankind Hp,32 Balb/c mice infected by Hp were randomly divided into 4 groups:Group 1(PPI trigeminy treatment group),Group 2 (Bacillus acidi lactici CL22 treatment group),Group 3 (Bacillus acidi lactici CL24 treatment group),and Group 4 (normal saline control group). Intragastric administration was given continuously for 10 days. Another 8 normal mice were chosen as Group 5(blank control group). All mice were killed after 4 weeks since last intragastric administration. Hp was detected by rapid urease test,Giemsa dying, and bacterial culture,and histopathologic changes in the gastric mucosa of mice were determined by H-E staining.@*RESULTS@#There were significant differences in pathohistologic scores in sinus ventriculi among the 5 groups (F = 7.932, P = 0.000). The scores in Group 1, Group 2, Group 3, and Group 5 were obviously lower than those in Group 4 (P 0.05). The pathohistologic score in Group 3 was obviously higher than that in Group 5 (P 0.05). There was significant difference in Hp eradication rates in sinus ventriculi among the 5 groups (chi2 = 16.923, P=0.002). The Hp eradication rates in Group 1 and 2 were obviously lower than those in Group 4 (P 0.05). There also were significant differences in Hp eradication rate in corpus ventriculi among the 5 groups (chi2 = 14.295, P=0.006). Of them, Group 1 and Group 2 were higher than Group 4 (P 0.05).@*CONCLUSION@#Bacillus acidi lactici strain CL22 can effectively inhibit and eradicate Hp in Balb/c mouse model with Hp infectious gastritis in vivo. The therapeutic effect of Bacillus acidi lactici strain CL22 is equal to PPI + antibiotics and could be another choice of nonjantibiotic treatment for Hp.


Subject(s)
Animals , Antibiosis , Physiology , Female , Gastritis , Microbiology , Helicobacter Infections , Microbiology , Therapeutics , Helicobacter pylori , Lactic Acid , Chemistry , Lactobacillus , Metabolism , Physiology , Male , Mice , Mice, Inbred BALB C , Random Allocation
19.
Article in Chinese | WPRIM | ID: wpr-640431

ABSTRACT

Objective To assess the changes on the whole night polysomnography (PSG) in patients with chronic fatigue syndrome(CFS). Methods The whole night PSGs were recorded from 24 patients with CFS and 33 normal subjects. Results Compared with normal subjects, patients with CFS showed significantly reduced total sleep duration ([488.7?21.7]min vs [515.9?31.7]min, P

20.
Article in Chinese | WPRIM | ID: wpr-683475

ABSTRACT

Objective To find a better composition substitution fluid for continuous venovenous hemofiltration (CWH) comparing with the Port's composition substitution fluid,and to evaluate its efficiency and prognosis on patients with dysfunction of glycometabolism and multiple organ dysfunction syndrome (MODS) caused by trauma. Method Totol of 134 patients in the Firist Affiliated Hospotal of the Fourth Miliatary Medicial University,with glycometabolism dysfunction and MODS caused by trauma between Janurary 2001 and December 2006 were treated with CVVH.They were divided into two groups:the Port's composition substitution fluid which glucose concentration was 59.1 mmoL/L (group A,59 cases) and the new substitution fluid which glucose concentration is 11.8 mmol/L (group B,75 cases).The changes of electrolytes,arterial blood gas analysis,blood glucose and osmolarity during CVVH were observed.Results The mortality in group A was significantly higher than that in group B (64.4 % vs 40.0 %,P = 0.005).Before treated with CVVH,there was no significant differences of APACHEⅡscores between group A and group B.Either in the survival patients or in non-survivors,the APACHEⅡscores in group B were significantly higher than that in group A (P

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