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1.
China Occupational Medicine ; (6): 468-471, 2021.
Article in Chinese | WPRIM | ID: wpr-923220

ABSTRACT

Coal miners are a high-risk occupational group of work-related musculoskeletal disorders(WMSDs). This disease not only causes a reduction in the quality of life of workers, loss work ability, and decline in work efficiency, but also becomes an important factor leading to increase social medical burden. At present, most of the evaluation of WMSDs of coal miners at home and abroad adopts the international general scale. Chinese scholars have developed a WMSDs questionnaire that meets the occupational characteristics of Chinese population based on the international general scale—the Chinese Musculoskeletal Disorders Questionnaire. The questionnaire has good reliability and validity, and is a reliable and effective tool for evaluating WMSDs. The prevalence of WMSDs in coal miners is relatively high. The occupational factors that affect WMSDs in coal miners are mainly physical load, postural load, mental load, work organization, and work environment. The individual factors are mainly age, working experience, and education level. It′s recommended to prevent and control the occurrence of coal miners′ WMSDs through multi-level intervention measures such as strengthening education, changing the working posture, and improving the working conditions of coal miners, and rationally arranging labor organizations.

2.
Article in Chinese | WPRIM | ID: wpr-905495

ABSTRACT

Objective:To evaluate the residual stepping ability in monkeys with spinal cord injury longitudinally. Methods:Four adult female monkeys were studied. Right hemisection of 10 mm spinal cord tissue was performed at the T7-9 segment. Gait tests of bipedal locomotion were performed before, and six weeks and twelve weeks after injury by VICON system. Gait cycle duration, amplitude of knee and ankle angles, and ratio of united parameters were obtained from successive stepping and were quantitative analyzed. Results:The coordination of bilateral hindlimbs was destroyed after spinal cord injury, and the right hindlimb showed obviously dragging. The gait cycle duration of the left hindlimb increased significantly (P < 0.001), and the amplitudes of knee and ankle angle significantly increased (P < 0.001) after spinal cord injury. The ratio of united parameters was not statistically different among all the time points (P > 0.05). The gait cycle duration of the left hindlimb was correlated with step length (r = 0.838, P = 0.001), step height (r = 0.726, P = 0.007) and amplitude of ankle angle (r = 0.766, P = 0.004), and the amplitude of ankle angle was correlated with step length (r = 0.627, P = 0.029). Conclusion:The gait pattern of monkey with spinal cord injury has been changed. The gait strategy of the uninjured side was adjusted compensatively after spinal cord injury to adapt the functional impairment of contralateral hindlimb.

3.
Article in Chinese | WPRIM | ID: wpr-772636

ABSTRACT

OBJECTIVE@#We aim to determine the thickness of the labial plate, the distance between the cement-enamel junction (CEJ) and alveolar crest, and the inclination angle of the long axis of healthy maxillary anterior teeth by using cone- beam computed tomography (CBCT).@*METHODS@#A total of 345 CBCT volumes obtained by Newtom VGI® CBCT were analyzed by using the NNT software. The digital measurements of the labial bone plate thickness at level 4 mm below the CEJ, the midpoint of tooth root and the radiological tooth apex, the distance between the CEJ and alveolar crest, and the angle between the long axis of the teeth and the long axis of alveolar process were obtained from the mid-sagittal planes of maxillary incisors and canines. Plate thickness 4 mm below the CEJ was measured, and values below ≥1 mm were recorded.@*RESULTS@#In the central incisor, 1) the angle between the long axis of the teeth and alveolar bone was 15.2°±6.2°, the distance between the CEJ and alveolar crest was (1.5±1.0) mm, labial bone plate thick-ness at 4 mm below the CEJ was (0.8±0.4) mm, the midpoint of tooth root was (0.6±0.4) mm, and the radiological tooth apex was (1.3±0.7) mm; in the lateral incisor, 16.2°±8.8°, (1.6±1.0) mm, (0.7±0.5) mm, (0.4±0.6) mm, and (1.1±0.7) mm, respectively; and in the canine, 19.0°±6.2°, (1.8±1.0) mm, (0.9±0.6) mm, (0.4±0.6) mm, and (1.2±0.7) mm, respectively. 2) The frequencies of plate thickness ≥1 mm were 28.3%, 25.8%, and 42.7% in the central incisor, lateral incisor, and canine, respectively. 3) The distance between the CEJ and alveolar crest was positively correlated with age. The correlation coefficients was 0.42 (P<0.01) in the central incisor, 0.50 (P<0.01) in the lateral incisor, and 0.62 (P<0.01) in the canine.@*CONCLUSIONS@#The thickness of labial bone plate is thin, the distance from CEJ to alveolar crest increases with age, and the long axis of the teeth is more inclined than the long axis of alveolar process. Knowledge of these special morphological characteristics can improve the safety and result for many dental procedures.


Subject(s)
Alveolar Process , Bone Plates , Cone-Beam Computed Tomography , Incisor , Maxilla
4.
Article in Chinese | WPRIM | ID: wpr-702480

ABSTRACT

Objective To observe the influence of spinal cord injury on lower limbs weight support capacity,and the changes with time. Methods Six adult female rhesus monkeys with thoracic(T7-9)right spinal cord hemi-section were measured the plantar pressure ratio of both lower limbs with Foot-Scan system before,and six and twelve weeks after operation. Results There was no statistical difference between both sides of limbs before operation(Z=-1.330,P>0.05),while the plantar pressure ratio was more on the left limbs six and twelve weeks after operation(Z>4.783,P<0.001).The plantar pressure ratio of right lower limb became less and less during observation(Z=3.191,P<0.001). Conclusion The weight support capacity of affected limbs is injured after spinal cord hemi-section in monkeys, and would become worse without intervention.

5.
Article in Chinese | WPRIM | ID: wpr-698557

ABSTRACT

BACKGROUND: Either good biocompatibility and biological activity of active biological materials or the potential of multidirectional differentiation of neural stem cells has great application prospect and value. OBJECTIVE: To investigate the effect of neurotrophic factor 3-chitosan active biomaterial scaffolds on the differentiation of neural stem cells and the expression of key proteins of the neurotrophic factor 3 signal pathway in vitro. METHODS: The neural stem cells were extracted and purified, and then divided into pure culture medium group, soluble neurotrophic factor 3 group, pure chitosan group, and neurotrophic factor 3-chitosan group for differentiation induction. The expression of TrkC, Akt / p-Akt and Erk/p-Erk in the neurotrophic factor 3 signaling pathway was detected by western blot after 6 hours of induction. After 7 days of induction, differentiation of neural stem cells was observed by immunocytochemistry of MAP2, MBP, and GFAP. After 14 days of induction, formation of neural network induced by neurotrophic factor 3-chitosan active biomaterials was observed by immunocytochemistry of MAP2, Synapsin-1, and PSD95. RESULTS AND CONCLUSION: The neurotrophic factor 3-chitosan group induced a high proportion of neural stem cells differentiated into neurons, with a ratio of 73.8%, which was significantly higher than that in the other three groups. Meanwhile, the proportion of cells differentiated into glial cells waslower than that in the other three groups. The expression of key proteins TrkC, p-Akt and p-Erk in the neurotrophic factor 3-chitosan group was higher than that in the other three groups. Meanwhile, neurotrophic factor 3-chitosan could induce the in vitro differentiation of neural stem cells to form neural network.

6.
Acta Physiologica Sinica ; (6): 106-114, 2018.
Article in Chinese | WPRIM | ID: wpr-687847

ABSTRACT

The present study was aimed to investigate the electrophysiological characteristics of hippocampal postnatal early development mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in rats. Forty-eight Wistar rats were divided into postnatal 0.5-, 1-, 2- and 3-month groups (n = 12). Spontaneous excitatory postsynaptic currents (sEPSCs) and field excitatory postsynaptic potentials (fEPSPs) mediated by AMPA receptors were recorded to evaluate the changes in the intrinsic membrane properties of hippocampal CA1 pyramidal neurons by using patch-clamp and MED64 planar microelectrode array technique respectively. The results showed that, during the period of postnatal 0.5-3 months, some of the intrinsic membrane properties of hippocampal CA1 pyramidal neurons, such as the membrane capacitance (Cm) and the resting membrane potential (RMP), showed no significant changes, while the membrane input resistance (Rin) and the time constant (τ) of the cells were decreased significantly. The amplitude, frequency and kinetics (both rise and decay times) of sEPSCs were significantly increased during the period of postnatal 0.5-1 month, but they were all decreased during the period of postnatal 1-3 months. In addition, the range of evoked fEPSPs in hippocamal CA1 region was significantly expanded, but the fEPSP amplitudes were decreased significantly during the period of postnatal 0.5-3 months. Furthermore, the evoked fEPSPs could be significantly inhibited by extracellular application of the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). These results suggest that AMPA receptor may act as a major type of excitatory receptor to regulate synaptic transmission and connections during the early stage of hippocampal postnatal development, which promotes the development and functional maturation of hippocampus in rats.

7.
Article in English | WPRIM | ID: wpr-289862

ABSTRACT

Objective To summarize the positive signs on multi-detector CT angiography (CTA) for active severe gastrointestinal bleeding (GIB).Methods We retrospectively evaluated the clinical records and CT images of 93 patients with active severe GIB confirmed by digital subtraction angiography (DSA),endoscopy or surgery. The positive CT signs indicating the locations and causes of the bleeding were recorded. Results Intraluminal extravasation of contrast material was identified in 44 cases (47.3%),vascular malformation was found in 22 cases (23.7%),gastrointestinal tumor was detected in 18 cases (19.4%),focal or segmental abnormal bowel mucosal enhancement was present in 7 cases (7.5%),and diverticulum with abnormal enhancement was found in 2 cases (2.2%). Conclusion Positive signs for active severe GIB on CT are diverse and thus should be carefully interpreted in clinical setting.


Subject(s)
Angiography, Digital Subtraction , Contrast Media , Gastrointestinal Hemorrhage , Diagnostic Imaging , Humans , Retrospective Studies , Tomography, X-Ray Computed
8.
Article in English | WPRIM | ID: wpr-281453

ABSTRACT

Objective To evaluate the role of multidetector computed tomography-angiography (MDCTA) for management of life-threatening spontaneous retroperitoneal haemorrhage (SRH).Methods A retrospective analysis of the medical records of all SRH patients admitted to our hospital from January 1, 2005 to November 31, 2014 was performed. SRH was defined as unrelated to invasive procedures, surgery, trauma, abdominal tumor or aortic aneurysm. Life-threatening SRH was defined as hemodynamic instability with hypotension. Sixteen cases met the inclusive criteria. Patients' characteristics, clinical presentation, imaging of MDCTA, treatment modalities, morbidity, and follow-up data were analyzed.Results The group of 16 patients included 11 men and 5 women, with a mean age of 51.5±16.4 years. All patients underwent MDCTA scan examinations, and active contrast media (CM) extravasation were found in 9 patients. Angiography was performed for 8 patients in the CM extravasation group. Bleeding vessels were found in 7 patients who underwent successful embolization. One patient in the CM extravasation group did not undergo angiography and died of bleeding. Seven patients without active CM extravasation underwent conservative management. Bleeding was controlled for all these 7 patients, and 1 patient died of multiple organ failure. Fourteen patients survived from SRH were successfully discharged. One patient died of heart failure during follow-up.Conclusion MDCTA was useful in the diagnosis of SRH and proved to be predictive for the outcome of conservative treatment and early intervention.

9.
Chinese Medical Journal ; (24): 1738-1742, 2015.
Article in English | WPRIM | ID: wpr-231700

ABSTRACT

<p><b>BACKGROUND</b>Dysfunctional spinal circuit may play a role in the pathophysiology of amyotrophic lateral sclerosis (ALS). The purpose of this study was to use F waves for assessment of segmental motoneuronal excitability following upper motor neuron (UMN) dysfunctions in ALS.</p><p><b>METHODS</b>We studied the F waves of 152 ulnar nerves recorded from abductor digiti minimi in 82 patients with ALS. Two groups of hands were defined based on the presence or absence of pyramidal signs in the same upper limb. The group with pyramidal signs in the upper limbs was designated as the P group, and the group without pyramidal signs in the upper limbs was designated as the NP group.</p><p><b>RESULTS</b>The mean (P < 0.001), median (P < 0.001) and maximum (P = 0.035) F wave amplitudes, mean (P < 0.001), median (P < 0.001) and maximum (P = 0.003) F/M amplitude ratio, index repeating neuron (P < 0.001) and index repeater F waves (P < 0.001) of the P group were significantly increased compared with the NP group. No significant differences were identified for F wave chronodispersion (P = 0.628), mean F wave latency (P = 0.151), minimum F wave latency (P = 0.211), maximum F wave latency (P = 0.199), F wave persistence (P = 0.738), F wave duration (P = 0.152), F wave conduction velocity (P = 0.813) and number of giant F waves (P = 0.072) between the two groups.</p><p><b>CONCLUSIONS</b>In this study, increased F wave amplitude, F/M amplitude ratio and number of repeater F waves reflected enhanced segmental motoneuronal excitability following UMN dysfunctions in ALS.</p>


Subject(s)
Adult , Aged , Amyotrophic Lateral Sclerosis , Female , Humans , Male , Middle Aged , Motor Neuron Disease , Motor Neurons , Physiology , Neural Conduction , Physiology , Ulnar Nerve , Physiology
10.
Chinese Medical Journal ; (24): 2228-2233, 2015.
Article in English | WPRIM | ID: wpr-335629

ABSTRACT

<p><b>BACKGROUND</b>Shikonin is a major active chemical component extracted from Lithospermi Radix, an effective traditional herb in various types of wound healing. Shikonin can accelerate granulomatous tissue formation by the rat cotton pellet method and induce neovascularization in granulomatous tissue. The purpose of the study was to investigate its mechanism of action in human skin cells.</p><p><b>METHODS</b>MTS assay was used to measure cell growth. The collagen type I (COL1 ) mRNA expression and procollagen type I C-peptide (PIP) production were detected by real-time quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Immunofluorescence and western blot analyses were carried out to investigate nuclear factor-κB (NF-κB) signaling pathway. Cell-based proteasome activity assay was used to determine proteasome activity.</p><p><b>RESULTS</b>In this study, we found that 10 μmol/L shikonin stimulated the growth of normal human keratinocytes and 1 μmol/L shikonin promoted growth of human dermal fibroblasts. However, shikonin did not directly induce COL1 mRNA expression and PIP production in dermal fibroblasts in vitro. In addition, 1 μmol/L shikonin inhibited translocation of NF-κB p65 from cytoplasm to nucleus induced by tumor necrosis factor-α stimulation in dermal fibroblasts. Furthermore, shikonin inhibited chymotrypsin-like activity of proteasome and was associated with accumulation of phosphorylated inhibitor κB-α in dermal fibroblasts.</p><p><b>CONCLUSIONS</b>These results suggested that shikonin may promote wound healing via its cell growth promoting activity and suppress skin inflammation via inhibitory activity on proteasome. Thus, shikonin may be a potential therapeutic reagent both in wound healing and inflammatory skin diseases.</p>


Subject(s)
Cell Proliferation , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Fibroblasts , Humans , Keratinocytes , NF-kappa B , Metabolism , Naphthoquinones , Pharmacology , Polymerase Chain Reaction , Proteasome Endopeptidase Complex , Skin , Cell Biology
11.
Chinese Medical Journal ; (24): 2295-2300, 2015.
Article in English | WPRIM | ID: wpr-335615

ABSTRACT

<p><b>BACKGROUND</b>The spectrum of abnormal behaviors in amyotrophic lateral sclerosis/motor neuron disease (ALS/MND) has been described, but its practical meaning, namely its impact on caregiver burden, has not been clearly documented in Chinese population. This study aimed to assess the distribution of abnormal behaviors in Chinese population, and to analyze the relationship between behavior changes and caregiver burden.</p><p><b>METHODS</b>Sixty-five patients with ALS/MND have been consecutively enrolled into registry platform of Peking Union Medical College Hospital. An investigation was performed to these patients and their caregivers using the revised ALS function rating scale, Frontal Behavioral Inventory-ALS version, the Frontal Assessment Battery, and the Caregiver Burden Inventory.</p><p><b>RESULTS</b>Twenty-eight (43.1%) patients displayed abnormal behaviors of varying degrees, with one fulfilling the diagnostic criteria of frontotemporal lobe degeneration. Irritability, logopenia, and inflexibility ranked top 3 of abnormal behavior list. Correlation analysis revealed that the degree of behavioral change and frontal cognitive status were significantly associated with caregiver burden, with more extensive impact from disinhibitive behaviors. Analysis of covariance analysis showed that after associated factors were corrected, caregivers of patients with moderate to severe behavior change reported significantly heavier developmental burden, physical burden, and total burden than those with no behavioral change.</p><p><b>CONCLUSIONS</b>Neurobehavioral symptoms could present in around 40% of Chinese patients with ALS/MND, and the distribution of these behaviors was also unique. Besides, abnormal behaviors were highly related to caregivers' burden.</p>


Subject(s)
Adult , Aged , Amyotrophic Lateral Sclerosis , Psychology , Behavioral Symptoms , Psychology , Caregivers , Psychology , Female , Humans , Male , Middle Aged , Quality of Life
12.
Article in English | WPRIM | ID: wpr-242829

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the placement of a long tube into the small intestine under fluoroscopic guidance and to evaluate its decompression effect on early postoperative small bowel obstruction (EPSBO).</p><p><b>METHODS</b>Fifty-four patients with EPSBO requiring decompression between April 2010 and July 2014 were enrolled in the study. Insertion of a long tube was guided by fluoroscopy. We first used the guide wire to pass the pylorus and then used the 10 Fr feeding tube as an exchangeable tube to put the superstiff wire into the duodenum. Finally the long tube could be passed over the guide wire through the pylorus into the intestine. The total procedure time, the radiation exposure time, and the incidence of complications were evaluated.</p><p><b>RESULTS</b>The long tubes passed into the jejunum on initial insertion for all patients, so the success rate of this technique was 100%. The long tube was inserted into ileum in 18 patients. The mean total procedure time was 34.4 ± 8.6 minutes, and the mean radiation exposure time 18.9 ± 6.8 minutes. A total of 47 patients (87%) experienced full recovery following long-tube decompression and without the need for surgical intervention.</p><p><b>CONCLUSIONS</b>Using the wire-exchange technique, it is easy to place a long tube into the small bowel under fluoroscopic guidance. This decompression method is safe and effective for management of EPSBO.</p>


Subject(s)
Adult , Aged , Decompression, Surgical , Methods , Female , Fluoroscopy , Humans , Intestinal Obstruction , General Surgery , Male , Middle Aged , Postoperative Complications , General Surgery , Retrospective Studies
13.
Article in English | WPRIM | ID: wpr-329831

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the value of multi-slice computed tomography angiography (MSCTA) in the diagnosis of visceral artery aneurysms (VAA).</p><p><b>METHODS</b>The imaging data of 123 VAA patients who received abdominal contrasted CT and MSCTA on the PACS workstation in our department from May 2008 to October 2010 were retrospectively analyzed. Based on the findings of comprehensive evaluation and using vessels as the analytic unit, we evaluated the sensitivity and specificity of enhanced axial images and post-processing images in detect the VAA.</p><p><b>RESULTS</b>Totally 172 VAAs were found in these 123 patients by the comprehensive evaluation, among which 107 were diagnosed by MSCTA.The results included 91 splenic artery aneurysms, 16 renal artery aneurysms, 8 multi-visceral artery aneurysms, 2 gastric artery aneurysms, 2 superior mesenteric artery aneurysms, 1 celiac trunk aneurysm, 1 gastroduodenal artery aneurysm, 1 pancreaticoduodenal artery aneurysm, and 1 hepatic artery aneurysm. The sensitivity and specificity of MSCTA in diagnosing TAA was 100% and 100%, respectively.</p><p><b>CONCLUSION</b>With different reconstruction methods, MSCTA can accurately and clearly display the location, shape, extent, aneurysmal wall, parent artery, and relationship with the adjacent vessels of VAAs.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Aneurysm , Diagnostic Imaging , Angiography , Methods , Female , Humans , Male , Middle Aged , Retrospective Studies , Sensitivity and Specificity , Tomography, Spiral Computed , Young Adult
14.
Article in Chinese | WPRIM | ID: wpr-329822

ABSTRACT

<p><b>OBJECTIVE</b>To summarize the computed tomography (CT) findings of adrenal hemangioma.</p><p><b>METHOD</b>The CT findings of 9 patients with pathologically proved adrenal hemangioma from June 2008 to March 2011 were retrospectively reviewed.</p><p><b>RESULTS</b>All the tumors were located unilaterally, with 4 in the left and 5 in the right. The tumors were 1.5-8.4 cm in size with complete capsule and well-defined contour. Among these nine tumors, six were round or ovoid, while the other three had irregular shapes. Plain scan showed mixed density in 8 and cystic change in 1. After contrast enhancement, six tumors manifested with inhomogeneous enhancement and the other three without enhancement. For three cases who underwent multi-phase enhanced CT scans, irregular enhancement at the periphery of the masses were showed in artery phase, and centripetal filling with contrast medium to the center of masses were showed in the portal and delayed phases.</p><p><b>CONCLUSIONS</b>Hemangioma is a rare benign tumor of the adrenal gland. CT findings are diverse, and the preoperative misdiagnosing rate can be high. Multi-phase enhanced CT scan may show characteristic features of adrenal hemangioma and help achieve correct preoperative diagnosis.</p>


Subject(s)
Adrenal Gland Neoplasms , Diagnostic Imaging , Adult , Female , Hemangioma , Diagnostic Imaging , Humans , Male , Middle Aged , Retrospective Studies , Tomography, X-Ray Computed , Methods
15.
Article in Chinese | WPRIM | ID: wpr-329792

ABSTRACT

<p><b>OBJECTIVE</b>To assess the feasibility and effectiveness of transarterial embolization for management of acute massive hemorrhage in patients with duodenal ulcer.</p><p><b>METHODS</b>Twenty-two patients with duodenal ulcer underwent transarterial embolization for acute massive hemorrhage in our hospital between January 2007 and December 2012. Embolic agents were coils and gelatin sponge. The clinical data and embolization procedures of these patients were retrospective analyzed.</p><p><b>RESULTS</b>Bleeding was controlled in 20 of 23 patients after the first embolization procedures. In the other 3 patients with rebleeding, one patient was successfully managed by repeat embolization and two patient underwent surgical treatment. The overall clinical success rate for acute hemorrhage after transarterial embolization was 91% (21/23). No severe complication occurred.</p><p><b>CONCLUSION</b>Transarterial embolization is safe and effective for acute massive hemorrhage in patients with duodenal ulcer.</p>


Subject(s)
Adult , Aged , Duodenal Ulcer , Embolization, Therapeutic , Methods , Female , Gastrointestinal Hemorrhage , Therapeutics , Humans , Male , Middle Aged , Retrospective Studies , Treatment Outcome
16.
Chinese Pharmaceutical Journal ; (24): 615-620, 2013.
Article in Chinese | WPRIM | ID: wpr-860412

ABSTRACT

OBJECTIVE: To evaluate the stability of epirubicin hydrochloride solutions for clinical use prepared from three different formulations (one concentrated solution and two powders for injection). METHODS: Epirubicin hydrochloride in three formulations was dissolved or diluted in 0.9% sodium chloride to prepare the test solutions with concentration of 0.4mg · mL-1. These solutions were stored at different temperatures and under different shading circumstances, and samples were collected periodically during the storage to evaluate the stability, including visual inspection, pH value and content of epirubicin. RESULTS: All epirubicin solutions were stable when stored at 2-8°C under dark condition for 40 d with average content of greater than 93.76%. When stored atroom temperature with or without light exposure for 40 d, only the solution diluted from the concentrated water solution for injection was stable, as the average content remained above 92.57%. While the solutions prepared from the other two powders for injection were not stable, as the average content fell below 90% at room temperature after 2-7 d. CONCLUSION: Epirubicin solutions prepared from three different formulations are stable when stored either at 2-8°C under dark condition for 40 d or at room temperature for 24 h. The solution prepared from the concentrated water solution for injection is more stable than the solutions prepared from the two powders for injections when stored at room temperature for a long period (40 d).

17.
Chinese Journal of Oncology ; (12): 196-200, 2012.
Article in Chinese | WPRIM | ID: wpr-335314

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the expression of BRCA1, ERCC1, TUBB3 and PRR13 mRNA and their relationship with clinical chemosensitivity in primary ovarian cancer, and to assess the predictive value of joint detection of both BRCA1 and ERCC1 genes for the treatment of primary ovarian cancer.</p><p><b>METHODS</b>Primary epithelial ovarian tumor samples were collected from 46 patients who underwent cytoreductive surgery. Real-time quantitative PCR was used to analyze the relative expression of BRCA1, ERCC1, TUBB3 and PRR13 mRNA in those cases. The correlation of clinical chemosensitivity and the test results was statistically analyzed. The efficacy of the joint prediction of clinical chemosensitivity by combining the two drug resistance gene detection was evaluated.</p><p><b>RESULTS</b>The BRCA1 mRNA relative expression logarithm in the clinical-resistant group was 0.673±2.143, and clinical-sensitive group -1.436±2.594 (P=0.008). The ERCC1 mRNA relative expression logarithm in the clinical-resistant group was -0.529±1.982 and clinical-sensitive group -3.188±2.601 (P=0.001). BRCA1 and ERCC1 expression level is negatively correlated with platinum-based chemosensitivity. The PRR13 expressions in the two groups were not significantly different (P=0.074), and the TUBB3 expressions between the two groups were also not significantly different (P=0.619). When the intercept point value BRCA1 mRNA expression logarithm was -0.6, the predictive sensitivity, specificity, positive predictive value and negative predictive value were 73.3%, 75.0%, 84.6% and 60.0%, respectively, with the best comprehensive assessment. When the intercept point value of ERCC1 mRNA expression logarithm was -1, the predictive sensitivity, specificity, positive predictive value and negative predictive value were 80.0%, 68.8%, 82.8% and 64.7%, respectively, with the best comprehensive assessment. The combination detection of BRCA1 and ERCC1 can improve the chemotherapeutic sensitivity, specificity, positive predictive value and negative predictive value to 86.7%, 68.8%, 83.9% and 73.3%, respectively.</p><p><b>CONCLUSIONS</b>BRCA1 and ERCC1 mRNA expression has a negative correlation with the clinical sensitivity of platinum-based chemotherapy. Combination detection of the two drug-resistance associated genes can improve the predictive efficacy of ovarian cancer chemosensitivity and beneficial to individual treatment of ovarian cancer.</p>


Subject(s)
Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , BRCA1 Protein , Genetics , Metabolism , CA-125 Antigen , Blood , Carboplatin , DNA-Binding Proteins , Genetics , Metabolism , Drug Resistance, Neoplasm , Endonucleases , Genetics , Metabolism , Female , Gene Expression Regulation, Neoplastic , Humans , Neoplasms, Glandular and Epithelial , Drug Therapy , Metabolism , General Surgery , Ovarian Neoplasms , Drug Therapy , Metabolism , General Surgery , Paclitaxel , RNA, Messenger , Metabolism , Repressor Proteins , Genetics , Metabolism , Tubulin , Genetics , Metabolism
18.
Chinese Journal of Oncology ; (12): 378-381, 2012.
Article in Chinese | WPRIM | ID: wpr-335275

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the clinical characteristics, influencing factors and outcome of recurrent patients with early stage bulky cervical carcinoma.</p><p><b>METHODS</b>Between January 1(st) 2000 and December 31(st) 2009, 76 patients with stage Ib2 and IIa2 bulky cervical carcinoma developed recurrence and (or) metastasis. The recurrence time, recurrence location, recurrence-related factors, treatment and survival were analyzed.</p><p><b>RESULTS</b>The median follow up was 44 months (9-137 months). The overall recurrence and (or) metastasis rate was 22.6%. The 1-, 1-2, 3-5 and 5-year recurrence and (or) metastasis rates were 38.2%, 27.6%, 30.3% and 3.9%, respectively. The 5-year survival rate of local recurrence was 34.5%, that of distant metastasis was 23.6%, and that of distant metastasis with synchronous pelvic recurrence was 11.1%, (P = 0.555). The 5-year survival rate of patients who received surgery plus chemotherapy, radiation plus chemotherapy and chemotherapy alone after recurrence and (or) metastasis were 53.3%, 30.7% and 24.6%, respectively (P = 0.686). Univariate analysis demonstrated that tumor recurrence and (or) metastasis in patients of the stage Ib2 and IIa2 bulky cervical carcinoma were influenced by the disease stage, pelvic lymph node metastasis, deep cervical stromal invasion, lymphovascular tumor thrombus and pathological types. Multivariate regression analysis demonstrated that pelvic lymph node metastasis, lymphovascular tumor thrombus and pathological types were the key factors affecting the recurrence and (or) metastases of the stage Ib2 and IIa2 bulky cervical carcinoma. Subgroup analysis showed that pelvic lymph node metastasis and stage were the main factors affecting the local recurrence in those patients, and the pathological type, vascular tumor thrombus and pelvic lymph node metastasis were the main factors affecting the distant metastasis.</p><p><b>CONCLUSIONS</b>Recurrence and(or) metastasis of early stage bulky cervical cancer are mostly happened within 2 years post operation. Patients with pelvic lymph node metastasis have high probability to develop local recurrence and distant metastasis. Patients with non-squamous cell carcinoma and lymphovascular tumor thrombus are more likely to develop distant metastasis. Neoadjuvant chemotherapy does not decrease local recurrence and distant metastasis in patients with stage Ib2 and IIa2 bulky cervical carcinoma. Individualized treatment is advised for recurrent patients.</p>


Subject(s)
Adenocarcinoma , Pathology , General Surgery , Therapeutics , Carcinoma, Squamous Cell , Pathology , General Surgery , Therapeutics , Chemotherapy, Adjuvant , Female , Follow-Up Studies , Humans , Hysterectomy , Lung Neoplasms , Drug Therapy , Lymph Node Excision , Lymph Nodes , Pathology , Lymphatic Metastasis , Multivariate Analysis , Neoadjuvant Therapy , Neoplasm Invasiveness , Neoplasm Recurrence, Local , General Surgery , Therapeutics , Neoplasm Staging , Pelvis , Radiotherapy, Adjuvant , Survival Rate , Tumor Burden , Uterine Cervical Neoplasms , Pathology , General Surgery , Therapeutics
19.
Article in English | WPRIM | ID: wpr-235570

ABSTRACT

<p><b>OBJECTIVE</b>Symptomatic predictors of influenza could assess risks and improve decisions about isolation and outpatient treatment. To develop such predictors, we undertook a prospective analysis of pandemic (H1N1) 2009 and seasonal influenza (H3N2) in patients attending fever clinics.</p><p><b>METHODS</b>From 1 May 2009 to 1 January 2010, all adult patients admitted to fever clinics for suspected influenza, confirmed by real time RT-PCR, were enrolled. Predictors of influenza virus infection were selected with logistic regression models. Measures of sensitivity, specificity, positive and negative likelihood ratios (LRs) were calculated to identify the best predictors.</p><p><b>RESULTS</b>The clinical features and routine blood test results of influenza (H1N1) 2009 and seasonal influenza were similar. The positive and negative LRs of current US CDC influenza-like illness (ILI) criteria were modest in predicting influenza infection. Our modified clinic predictors improved the ability of the positive and negative LRs to recognize pandemic (H1N1) 2009 and seasonal influenza. The revised criteria are: fever >38 °C accompanied by at least one of the following-cough, arthralgia or relative lymphopenia.</p><p><b>CONCLUSION</b>Patients with symptoms and signs that meet the new criteria are likely to have influenza and timely antiviral therapy may be appropriate. In addition, physicians should ascertain if influenza is circulating within the community or if there is a contact history of influenza and combine this information with the newly developed criteria to clinically diagnose influenza.</p>


Subject(s)
Adult , China , Epidemiology , Female , Humans , Influenza A Virus, H1N1 Subtype , Influenza A Virus, H3N2 Subtype , Influenza, Human , Diagnosis , Epidemiology , Virology , Logistic Models , Male , Multivariate Analysis , Pandemics , Predictive Value of Tests , Prospective Studies , Young Adult
20.
Chinese Journal of Oncology ; (12): 935-938, 2010.
Article in Chinese | WPRIM | ID: wpr-293449

ABSTRACT

<p><b>OBJECTIVE</b>Vulvar epithelioid sarcoma is a rare, undifferentiated soft-tissue sarcoma, with a high rate of local relapse, regional nodal spread and distant metastases. The aim of this study was to investigate the clinical features, diagnosis, treatment and prognosis of this malignancy.</p><p><b>METHODS</b>We studied the clinicopathologic features of 20 cases of vulvar epithelioid sarcoma, of which 4 cases were admitted to our hospital from 1999 to 2009. All of the patients received radical local excision with inguinofemoral lymphadenectomy. Seven patients were treated without adjuvant therapy. Seven patients received postoperative radiotherapy only and three underwent chemotherapy. Chemotherapy plus radiotherapy were given postoperatively in three.</p><p><b>RESULTS</b>The patients ranged in age from 23 to 80 years (median: 36 y). The tumors ranged from 1 to 10 cm in their greatest diameter (median: 5.1 cm). All cases showed immunoreactivity for both vimentin and cytokeratin. Follow-up information on all 20 patients was available, and covered periods ranging from 3 to 104 months.11 patients were alive with no evidence of disease. 2 patients developed lymph node metastases but alive. 7 patients had died of the disease. Survival of the early stage (I-II) patients was significantly longer than those in the advanced stage (III-IV) (median, 21 vs. 6 months, P < 0.01). There was no significant difference between survival of patients with or without inguinofemoral lymphadenectomy (median, 11.5 vs. 6 months, P = 0.086).</p><p><b>CONCLUSIONS</b>Because of the relatively frequent misdiagnosis, a differential diagnosis combined with immunohistochemistry is needed to determine an early and accurate diagnosis. The tumor markers exhibiting immunoreactivity includ vimentin, epithelial membrane antigen (EMA) and cytokeratin (CK). Radical local excision with adequate margin (at least 2 cm) and bilateral inguinofemoral lymphadenectomy is effective for the treatment of vulvar epithelioid sarcoma. The role of adjuvant therapy, chemotherapy and radiation remains unclear but merits consideration.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Chemotherapy, Adjuvant , Female , Follow-Up Studies , Humans , Keratins , Metabolism , Lymph Node Excision , Lymphatic Metastasis , Middle Aged , Mucin-1 , Metabolism , Neoplasm Staging , Radiotherapy, Adjuvant , Retrospective Studies , Sarcoma , Drug Therapy , Metabolism , Pathology , Radiotherapy , General Surgery , Soft Tissue Neoplasms , Drug Therapy , Metabolism , Pathology , Radiotherapy , General Surgery , Survival Rate , Vimentin , Metabolism , Vulva , General Surgery , Vulvar Neoplasms , Drug Therapy , Metabolism , Pathology , Radiotherapy , General Surgery , Young Adult
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