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1.
Article in Chinese | WPRIM | ID: wpr-873294

ABSTRACT

Objective::Because traditional methods are difficult to identify the fermentation mycelium, DNA barcoding technology was used to quickly identify the raw material strain Paecilomyces hepiali of Jinshuibao capsules and related products. Method::A total of 168 samples of 8 species of P. hepiali and its confusable species were identified by internal transcribed spacer (ITS) sequences, and based on the ITS sequences, P. hepiali specific primers were designed to quickly identify the related products. Result::The length of ITS sequences in 44 P. hepiali samples from different sources was 499 bp and there was no mutation site. It was shown that P. hepiali could be distinguished from 7 confusable species based on ITS sequences. The specific primer (ITS-BF/ITS-BR) of P. hepiali designed by ITS sequences could be amplified to obtain a short fragment of 102 bp in length, which could be used to rapidly identify P. hepiali from other confusable species, and to distinguish relevant products in the market. Conclusion::The rapid identification of P. hepiali and its related products can be achieved through the ITS sequences and specific primers, which provides a reference for the production and quality control of Jinshuibao capsules.

2.
Article in Chinese | WPRIM | ID: wpr-775985

ABSTRACT

Objective To validate the Union Physio-Psycho-Social Assessment Questionnaire(UPPSAQ-70)and test its validity and reliability.Methods From April,2013 to July,2018,patients were asked to finish the computer evaluation of UPPSAQ-70 and Symptom Checklist 90(SCL-90)in Peking Union Medical College Hospital(PUMCH).Confirmatory factor analysis(CFA)was conducted on the SPSS 17.0,and the number of fixed factors was 8 factors and 3 factors.Amos 23.0 was used to verify the original 8-factor model,8-factor revision model,3-factor model,3-factor revision model,and single-factor model.Each factor of SCL-90 was used as the calibration standard to calculate the correlation coefficient between factors.The retest reliability was tested by the outpatients in PUMCH in July,2018.Results Exploratory factor analysis indicated that the 8-factor revised model included:depression,anxiety and fatigue,sleep,physical discomfort,sexual function,happiness and satisfaction,hypochondria,and social anxiety.The 3 factors revised model included that:psychological,physiological and social dimension.Confirmatory factor analysis indicated that the 8-factor modified model was superior to the 3-factor model and the single-factor model: =10 410.4,=1862,RMSEA=0.07,CFI=0.753,and NFI=0.715.With SCL-90 as the standard criteria,except the low correlation coefficient between emotional scale and depression(=0.600)and anxiety(=0.520),the correlation coefficients of other symptoms were below 0.5.The chronbach's between each factor and total score of UPPSAQ-70 was between 0.823 and 0.904,and the Chronbach's coefficient of the whole scale was between 0.954 and 0.956 after each item was deleted.The retest reliability of the scale of 32 participants Chronbach's was 0.847.Each item of the scale measured between one week was significantly correlated(<0.05). Conclusion UPPSAQ-70 is a good scale for evaluating overall health status and is especially feasible in general hospitals.


Subject(s)
Factor Analysis, Statistical , Humans , Psychological Tests , Reference Standards , Psychometrics , Reproducibility of Results , Surveys and Questionnaires
3.
Article in Chinese | WPRIM | ID: wpr-699368

ABSTRACT

Objective:To explore changes of serum levels of chitinase protein-40(YKL-40),P-selectin and high sensi-tive C reactive protein(hsCRP)in patients with acute coronary syndrome(ACS)and their correlation.Methods:A total of 160 patients with coronary heart disease(CHD),including 80 ACS cases and 80 cases with stable angina pec-toris(SAP),were selected.Another 80 non-CHD patients were enrolled as normal control group.Serum levels of YKL-40,P-selectin and hsCRP were measured by enzyme linked immunosorbent assay and compared among all groups.Results:Compared with normal control group and SAP group,there were significant rise in serum levels of YKL-40[(20.92 ± 3.19)ng/ml vs.(45.18 ± 3.96)ng/ml vs.(61.84 ± 3.51)ng/ml],P-selectin[(65.01 ± 16.18) μg/L vs.(97.58 ± 31.43)μg/L vs.(137.41 ± 48.26)μg/L]and hsCRP[(6.91 ± 3.68)mg/L vs.(8.32 ± 4.21) mg/L vs.(30.01 ± 11.05)mg/L]in ACS group,P=0.001 all.Linear correlation analysis indicated that serum P-selectin and hsCRP levels was significant positively correlated with serum YKL-40 level(r= 0.46,0.041, P=0.021,0.015).Conclusion:Serum levels of YKL-40,P-selectin and hsCRP significantly rise in ACS patients,sug-gesting that these indexes are related to atherosclerotic plaque instability,which can be used as serological indexes judging atherosclerotic plaque instability.

4.
Article in English | WPRIM | ID: wpr-277962

ABSTRACT

Objective To analyze liaison psychiatric service in a geriatric ward in a big general hospital and explore the way to improve accessibility of geriatric psychiatric service in general hospitals. Methods Elderly inpatients aged 65 years old or more admitted to a geriatrics ward in Peking Union Medical College Hospital in 7 years duration (January 2008 to December 2014) were enrolled in the study. Liaison psychiatrists attended ground round combined with psychiatric consultation from January 2008 to December 2009 (T1 period). Comprehensive geriatric assessment,geriatric interdisciplinary team services and psychiatric consultation were conducted from January 2010 to December 2014 (T2 period). Consultation rate,reasons for referral,psychiatric diagnoses,length of stay,and medical expense were compared between different periods.Results Among 1230 geriatric inpatients,383 patients were enrolled in liaison psychiatric service,and 511 individual consultations happened.The consultation rate for T1 and T2 were 19.7% and 33.8%,respectively (P=0.000).The dominating reasons for referral were current emotional symptoms (30.4%) and current psychiatric symptoms (28.3%) in T1 and current emotional symptoms (65.3%) and medically unexplained symptoms (12.8%) in T2 (P=0.000). For length of stay,consultation group lasted for (199.2±40.0) days,and non-consultation group lasted for (71.3±16.6) days(P=0.004) in T1;(22.0±2.4) days and (22.6±1.6) days(P=0.834) in different groups in T2 respectively. The medical expense were (243 000±44 000) RMB for consultation group and (79 000±18 000) RMB for non-consultation group(P=0.040) in T1 and (18 000±2 000) RMB and (21 000±1 000) RMB (P=0.302) in different groups in T2 respectively. The prevalence rate for psychiatric disorders revealed by liaison psychiatrists was 15.8% in T1 and 29.8%in T2 (P=0.000) in the geriatric ward. Conclusions Elderly inpatients are vulnerable population for psychiatric disorders. Therefore,psychiatrist should be an important member in geriatric interdisciplinary team. Combination of comprehensive geriatric assessment,psychiatric consultation and geriatric interdisciplinary team is very efficient model in general hospital settings to improve the accessibility of psychiatric services in elderly in China.


Subject(s)
Aged , China , Female , Geriatric Assessment , Geriatrics , Hospitalization , Hospitals, General , Humans , Inpatients , Length of Stay , Male , Mental Disorders , Diagnosis , Psychiatric Department, Hospital , Referral and Consultation , Retrospective Studies
5.
Chinese Medical Journal ; (24): 2666-2669, 2016.
Article in English | WPRIM | ID: wpr-230903

ABSTRACT

<p><b>BACKGROUND</b>Recrossing the compromised side branch (SB) with a balloon is sometimes technically challenging. The aim of this study was to evaluate whether in-stent anchoring (ISA) is safe and effective to facilitate SB balloon delivery for final kissing.</p><p><b>METHODS</b>One hundred and fifty-nine consecutive patients were included (166 bifurcation lesions) in this prospective, single-center registry. ISA was used as a bailout method after unsuccessful SB crossing using conventional techniques, including low-profile balloons. Technique success was defined as SB balloon delivery and final kissing.</p><p><b>RESULTS</b>Kissing-balloon delivery was successfully performed with conventional strategies in 149 of 166 lesions (89.8%). In the remaining 17 lesions (10.2%), recrossing of the main vessel stent strut was not successful; therefore, ISA was attempted. The balloon successfully crossed the stent struts, and final kissing was achieved in 15 of 17 lesions (88.2%). Total final kissing was achieved in 164 of 166 lesions (98.8%), with success rates of 100% in the single-stent group and 97.6% in the two-stent group. Two cases without balloon delivery had complex bifurcation lesions with severe calcification. There was no vessel dissection in the anchoring zone.</p><p><b>CONCLUSIONS</b>ISA is safe and effective for recrossing stent struts when conventional low-profile balloons have failed. However, large-scale trials are warranted for further evaluation.</p>


Subject(s)
Aged , Angioplasty, Balloon, Coronary , Methods , Coronary Angiography , Methods , Coronary Artery Disease , Therapeutics , Drug-Eluting Stents , Female , Humans , Male , Middle Aged , Prospective Studies , Registries , Treatment Outcome
6.
Article in Chinese | WPRIM | ID: wpr-285998

ABSTRACT

<p><b>OBJECTIVE</b>To assess the validity of Complex Figure Test(CFT)in differentiating Alzheimer's disease(AD)from non-dementia.</p><p><b>METHODS</b>Using CFT,we tested 183 AD patients(AD group),1283 cognitively intact individuals(normal control group),and 134 individuals suffered from other diseases that could be easily confused with dementia(confused control group).</p><p><b>RESULTS</b>The CFT score was 38.7±0.2 in the normal control group,35.3±0.8 in confused control group,23.7±0.8 in mild AD group,and 13.2±1.1 in moderate AD group after adjusted for educational level,age,and sex(all P<0.05).With the 5(th) percentage of the overall score as cutoff point,this tool showed a sensitivity of 73.8% and a specificity of 93.8% in differentiating AD from non-dementia.</p><p><b>CONCLUSION</b>CFT is a sensitive and specific tool in the differentiation of AD from non-dementia.</p>


Subject(s)
Aged , Aged, 80 and over , Alzheimer Disease , Diagnosis , Diagnosis, Differential , Female , Humans , Male , Middle Aged , Neuropsychological Tests , Sensitivity and Specificity
7.
Article in English | WPRIM | ID: wpr-352916

ABSTRACT

<p><b>OBJECTIVE</b>To assess the validity of World Health Organization-University of California-Los Angeles Auditory Verbal Learning Test (WHO-UCLA AVLT) in the diagnosis of Alzheimer's disease (AD).</p><p><b>METHODS</b>Using WHO-UCLA AVLT, we assessed 183 AD patients (AD group),1283 subjects with normal cognitive status (normal control group), and 134 individuals suffered from other diseases easy to be confused with AD (confused control group).</p><p><b>RESULTS</b>The AVLT score was 40.9∓0.3 in normal control group, 30.7∓0.9 in confused control group, 16.6∓1.0 in mild AD group, and 10.2∓1.2 in moderate AD group after adjustment for educational level, age, sex, and rural/urban residence (all P<0.05). With the 5th percentage of the overall score as the cutoff point, this tool showed a sensitivity of 86.3% and a specificity of 93.3%.</p><p><b>CONCLUSION</b>WHO-UCLA CVLT is highly sensitive and specific in the diagnosis of AD.</p>


Subject(s)
Aged , Aged, 80 and over , Alzheimer Disease , Diagnosis , Female , Humans , Male , Middle Aged , Neuropsychological Tests , Sensitivity and Specificity , Verbal Learning
8.
Article in Chinese | WPRIM | ID: wpr-282670

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of carvedilol combined with perindopril on Ca(2+) pump activity and the density of Ca(2+)-release channel ryanodine receptor (RyR2) in the myocardial sarcoplasmic reticulum (SR) in rats with chronic heart failure caused by myocardial infarction.</p><p><b>METHODS</b>Rat models of chronic heart failure established by left coronary artery ligation were divided into different groups and treated with carvedilol (6 mg.kg(-1).d(-1)), perindopril (4 mg.kg(-1).d(-1)), terazosin (2 mg.kg(-1).d(-1)), or the combination of carvedilol (6 mg.kg(-1).d(-1)) and perindopril (4 mg.kg(-1).d(-1)) for 9 weeks. Another 12 rats with sham operation served as the sham-operated group. The hemodynamic parameters, activity of SR Ca(2+) pump, and RyR2 density were determined.</p><p><b>RESULTS</b>Compared with shame-operated group, the rats with chronic heart failure showed significantly increased left ventricular end-diastolic pressure (LVEDP) (P<0.01) and decreased +dP/dtmax, -dp/dtmax, activity of SR Ca(2+) pump and density of RyR2 (P<0.01). Both monotherapies with carvedilol and perindopril attenuated the increment of LVEDP, and significantly increased +dp/dtmax, -dp/dtmax, activity of SR Ca(2+) pump and density of RyR2 (P<0.01). Combined treatment even further enhanced the therapeutic effects, whereas terazosin produced no obvious effect. The activity of SR Ca(2+) pump was strongly correlated to +dp/dtmax and -dp/dtmax (r=0.596 and 0.684, respectively, P<0.01).</p><p><b>CONCLUSION</b>Prolonged treatment with beta-blocker carvedilol in combination with ACE inhibitor perindopril may improve the hemodynamic parameters, enhance Ca(2+) pump activity and increase the density of RyR2 of myocardial SR more effectively than either monotherapy in preventing and treating chronic heart failure following myocardial infarction.</p>


Subject(s)
Animals , Calcium , Metabolism , Carbazoles , Pharmacology , Therapeutic Uses , Drug Therapy, Combination , Heart Failure , Drug Therapy , Metabolism , Male , Myocardial Infarction , Metabolism , Perindopril , Pharmacology , Therapeutic Uses , Propanolamines , Pharmacology , Therapeutic Uses , Rats , Rats, Wistar , Ryanodine Receptor Calcium Release Channel , Sarcoplasmic Reticulum , Metabolism
9.
Acta Pharmaceutica Sinica ; (12): 480-485, 2009.
Article in English | WPRIM | ID: wpr-278235

ABSTRACT

This study investigated the effect of phloretin (Ph) on the proliferation, activation, and cell-cycle distribution of mouse T lymphocytes and NO production and phagocytosis of macrophages. Carboxyfluorescein diacetatesuccinimidyl ester (CFDA-SE) staining plus flow cytometry assay was employed to obtain the proliferation-related index (PI) of lymphocytes. The expression levels of CD69 and CD25 on T lymphocytes stimulated with Con A were evaluated with flow cytometry after staining with fluorescent monoclonal antibody. Cell-cycle distribution of T lymphocytes was analyzed by propidium iodide staining. Griess kit was used to evaluate the NO production and fluorescent microbeads were used to analyze the phagocytosis ability of macrophages. Our results showed that phloretin (40, 60, and 80 micromol x L(-7)) significantly inhibited the proliferation of T lymphocytes and the PI reduced from 1.41 +/- 0.13 to 1.34 +/- 0.16, 1.19 +/- 0.12 and 1.07 +/- 0.06, respectively. Phloretin significantly inhibited the expression of CD69 and CD25 (P < 0.01). The cell cycle distribution analysis showed that phloretin could induce a cell cycle arrest at G0/G1 phase. NO production of LPS +IFN-gamma group of macrophages was (26.72 +/- 3.57) micromol x L(-1), and was significantly reduced by phloretin (P < 0.01). And phagocytosis rate of macrophages was significantly reduced by phloretin (P < 0.01). The results demonstrate that phloretin might be developed into a new immuosuppressive drug.


Subject(s)
Animals , Anti-Inflammatory Agents , Pharmacology , Antigens, CD , Metabolism , Antigens, Differentiation, T-Lymphocyte , Metabolism , Cell Cycle , Cell Proliferation , Female , Immunosuppressive Agents , Pharmacology , Interleukin-2 Receptor alpha Subunit , Metabolism , Lectins, C-Type , Metabolism , Macrophages , Physiology , Bodily Secretions , Mice , Mice, Inbred BALB C , Nitric Oxide , Bodily Secretions , Phagocytosis , Phloretin , Pharmacology , T-Lymphocytes , Cell Biology , Allergy and Immunology
10.
Article in Chinese | WPRIM | ID: wpr-283786

ABSTRACT

<p><b>OBJECTIVE</b>To develvp a RP-HPLC method for the determination of flavonoids in fifteen kinds of flowers such as Iris lacteal pall, prunus persica and rosa chinensis.</p><p><b>METHOD</b>The contents of quercetin, kaempferol and isorhamntin in fifteen kinds of flowers were extracted with methanol. The analysis was performed on a Kromasil C18 column (4.6 mm x250 mm, 5 microm) with methanol-0.1% phosphoric acid (50:50) as mobile phase.</p><p><b>RESULT</b>The quercetin, kaempferol and isorhamntin were separated well, and the result shows that the content of quercetin in the Iris lactea Pall was the highest (1.536%), the contene of kaempferol in Persica persice was the highest (0.572%), and the content of isorhamntin in chrysamthemum morifolium was up to 0.290%.</p><p><b>CONCLUSION</b>The contents of flavonoids in these flowers were by determined RP-HPLC for the first time and the method can be used for quantitative determination of flavonoids in the flowers.</p>


Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Flavonoids , Chemistry , Flowers , Chemistry , Iris Plant , Chemistry , Kaempferols , Chemistry , Prunus , Chemistry , Quercetin , Chemistry , Rosa , Chemistry
11.
Chinese Journal of Oncology ; (12): 129-133, 2008.
Article in Chinese | WPRIM | ID: wpr-348152

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the efficacy and safety of subcutaneous injection of recombinant human interleukin-2 (Proleukin) in the treatment of metastatic renal cell carcinoma (RCC).</p><p><b>METHODS</b>Forty-one patients with pathologically confirmed metastatic RCC after radical nephrectomy were enrolled into this study. Two or four consecutive cycles of subcutaneous injection of rhLL-2 were given, with each cycle duration of five weeks consisting of 4 weeks of treatment and one week of rest. The rhLL-2 was injected twice daily subcutaneously at a dose of 9 MIU on D1-D5 during week one, then 9 MIU twice daily on D1-D2 and followed by 9 MIU daily on D3-D5 during week 2-4. Patients were evaluated after the second cycle of treatment. If an objective response or stable disease was observed, the patient would receive another two cycles of treeatment.</p><p><b>RESULTS</b>Of the 41 patients, the overall objective response rate was 17.1% (95% confidence interval, 5.6% to 28.6%) with a complete response (CR) rate of 0.0% and partial response rate (PR) of 17.1%. However, nineteen patients (46.3%) still had a stable disease (SD), and 15 (36.6%) had progressed disease (PD). The disease control rate was 63.4% and the median time to progression (mTTP) was 6 months. The 1-year survival rate was 71.2% with a median overall survival (mOS) rate of 22.5 months. Among 36 PP population, the overall objective response rate was 19.4% (95% confidence interval, 6.5% to 32.3%) with CR rate of 0.0% and PR rate of 19.4%. Sixteen patients(44.4%) had stable disease, and 13 (36.1%) progressed disease. The disease control rate was 63.9%. The 1-year survival rate was 66.7% with a median time to progression of 6 months. The median overall survival (mOS) had not reached yet. The follow-up data showed that the long term survival of the patient who responsed to the IL-2 therapy can be prolonged. Severe toxicity (> or = grade III) was rarely observed. Grade I or II toxicities such as fatigue (100.0%) and fever (82.9%) were frequently observed but reversible.</p><p><b>CONCLUSION</b>Subcutaneous injection of recombinant human interleukin-2 may prolong the survival of patients with a metastatic renal cell carcinoma. This regimen is tolerable with rare severe toxicities.</p>


Subject(s)
Adult , Aged , Antineoplastic Agents , Therapeutic Uses , Carcinoma, Renal Cell , Drug Therapy , General Surgery , Disease Progression , Fatigue , Female , Fever , Follow-Up Studies , Humans , Injections, Subcutaneous , Interleukin-2 , Therapeutic Uses , Kidney Neoplasms , Drug Therapy , Pathology , General Surgery , Lung Neoplasms , Male , Middle Aged , Nephrectomy , Proportional Hazards Models , Recombinant Proteins , Therapeutic Uses , Remission Induction , Survival Rate
12.
Chinese Journal of Cardiology ; (12): 714-717, 2008.
Article in Chinese | WPRIM | ID: wpr-355906

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the correlation between the number and activity of circulating endothelial progenitor cells (EPCs), insulin resistance and severity of coronary lesions in patients with coronary artery disease (CAD).</p><p><b>METHODS</b>Patients with coronary angiography evidenced CAD were divided in insulin resistance group (IR, n = 25) and insulin sensitive group (IS, n = 44) according to insulin level, 25 health volunteers served as control. Circulating EPCs were marked as KDR/CD133+ cells via fluorescence-activated cell sorter analysis. EPCs were also isolated from peripheral blood and cultured in vitro for 7 days, identified by DiI-acLDL uptake and lectin staining methods. EPCs migration activities were determined by modified Boyden chamber assay, EPCs proliferation activities were determined by MTT assay.</p><p><b>RESULT</b>Circulating EPCs number was significantly lower in IR group compared with IS group [(0.34 +/- 0.08) per thousand vs. (0.47 +/- 0.09) per thousand, P < 0.01] and control group (P < 0.05). Both insulin resistance index (r = -0.291, P = 0.01)and Gensini score (r = -0.3984, P = 0.006)were negatively correlated with number of circulating EPCs. Proliferation and migration capacities of EPCs were also significantly lower in IR group compared to those in IS group (all P < 0.05) and control group (all P < 0.05).</p><p><b>CONCLUSIONS</b>Insulin resistance/hyperinsulinemia could aggravate severity of coronary artery lesions via reducing the number and activities of circulating EPCs in patients with CAD.</p>


Subject(s)
Aged , Blood Cell Count , Cell Adhesion , Cell Movement , Cell Proliferation , Cells, Cultured , Coronary Angiography , Coronary Artery Disease , Blood , Pathology , Endothelial Cells , Cell Biology , Female , Humans , Insulin Resistance , Male , Middle Aged , Stem Cells , Cell Biology
13.
Chinese Journal of Cardiology ; (12): 435-438, 2008.
Article in Chinese | WPRIM | ID: wpr-243762

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effects of C reactive protein (CRP) on endothelial progenitor cell (EPCs) function.</p><p><b>METHODS</b>Mononuclear cells (MNCs), isolated from bone marrow by density gradient centrifugation combined with adherent cell filtration, were plated on fibronectin coated culture dishes. After 7 days, adherent cells were cultured with different concentrations of CRP (0, 5, 10, 15, 20 microg/ml) for 48 hours. EPCs proliferation and migration ability were observed and adhesion assay was performed. The eNOS mRNA expression of EPCs were measured by RT-PCR.</p><p><b>RESULTS</b>The number of EPCs in CRP groups (10, 15, 20microg/ml) was obviously lower than that in control group (54 +/- 3, 47 +/- 3, 39 +/- 5 vs.60 +/- 3, P < 0.01). EPCs proliferation capacity was inhibited in CRP groups (10, 15, 20 microg/ml) compared with that in control group (0.297 +/- 0.036, 0.273 +/- 0.013, 0.259 +/- 0.035 vs. 0.345 +/- 0.014, P < 0.01). EPCs migration capacity was inhibited significantly in CRP groups (5, 10, 15, 20 microg/ml) than that in control group (28 +/- 2, 22 +/- 3, 19 +/- 3, 16 +/- 2 vs. 30 +/- 2, P < 0.05). EPCs adhensive number was lower in CRP groups than that in control group (11 +/- 2, 9 +/- 2, 6 +/- 2, 5 +/- 1 vs. 12 +/- 2, P < 0.05). The mRNA expressions of eNOS in CRP groups were significantly lower in control group. And compared with control group, NOS activity decreased significantly in CRP groups (10, 15, 20 microg/ml) (57.44 +/- 3.25, 48.37 +/- 3.86, 36.82 +/- 4.89 vs. 68.56 +/- 2.82, P < 0.01).</p><p><b>CONCLUSION</b>CRP could both reduce EPCs number and inhibit EPCs functions.</p>


Subject(s)
Animals , Bone Marrow Cells , Cell Biology , C-Reactive Protein , Pharmacology , Cells, Cultured , Endothelial Cells , Metabolism , Male , Nitric Oxide Synthase Type III , Metabolism , Rats , Rats, Sprague-Dawley , Stem Cells , Cell Biology
14.
Chinese Journal of Burns ; (6): 265-268, 2007.
Article in Chinese | WPRIM | ID: wpr-347691

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of endogenous heat shock protein 90 (HSP90) on the AKT signaling pathway of hypoxic cardiomyocytes.</p><p><b>METHODS</b>The hypoxia model of neonatal rat cardiomyocyte was established. The cells were randomly divided into normal control, hypoxia, Geldanamycin (GA, with hypoxia after Geldanamycin treatment) groups. The myocardial cell activity and the expression of endogenous HSP90 and AKT were determined with MTT and Western blotting, respectively at 1, 3, 6, 12, 24 and 48 post-hypoxia hours (PHH). The apoptotic index (AI) of cardiomyocytes were determined with TUNEL method at 24 PHH.</p><p><b>RESULTS</b>(1) At 24 and 48 PHH, the activity of cardiomyocytes in hypoxia group and GA group were evidently lower than that in control group (P < 0.05). The activity of cardiomyocyte in GA group began to decrease at 12 PHH, and it was obviously lower than that in hypoxia group at 48 PHH (P < 0.05). (2) At 24 PHH, the AI in hypoxia group (10.7 +/- 1.2)% was obviously higher than that in normal control group [(1.9 +/- 0.3)%, P < 0.05], while it was obviously lower than that in GA group [(26.3 +/- 5.3)%, P < 0.01]. (3) The expression of endogenous HSP90 and AKT in hypoxia and GA groups were markedly increased compared with that of normal controls at 12 PHH, and it decreased at 24 PHH in hypoxia and GA groups, especially in the latter.</p><p><b>CONCLUSION</b>Endogenous HSP90 plays important roles in maintaining the cardiomyocyte activity, and its level might affect the expression of AKT.</p>


Subject(s)
Animals , Cell Hypoxia , Cells, Cultured , Disease Models, Animal , HSP90 Heat-Shock Proteins , Metabolism , Myocytes, Cardiac , Metabolism , Proto-Oncogene Proteins c-akt , Metabolism , Rats , Signal Transduction
15.
Chinese Journal of Burns ; (6): 409-412, 2007.
Article in Chinese | WPRIM | ID: wpr-347665

ABSTRACT

<p><b>OBJECTIVE</b>To investigate whether the third-party dendritic cells (DC) incubated with donor's antigens possess the similar immune functions with donor derived immature DC.</p><p><b>METHODS</b>Female C57 BL/6, BALB/c and Kunming mice were used as skin transplant donors, recipients and third-party, respectively. Forty BALB/c mice were randomly divided into A (normal control), B (cyclophosphamide administration), C (CTX and donor derived immature DC), D (CTX and third-party immature DC), E (CTX and third-party DC loaded with donor's antigens) groups, with 8 mice in each group. The mice in group B, C, D and E were given CTX (200 mg/Kg) 4 days before operation, while those in group A were given equivalent amount of normal saline(NS). Then in group C, D and E, DC at a dose of 1 x 10(7)/ml were intraperitoneally injected with 2 days before grafting and 12 days after operation, but the mice in group A and B were given NS in the same manner. Mean survival time (MST) of skin grafts was recorded, and biopsies of grafts on 5 and 10 post-operation days (POD) were harvested for histologic examination.</p><p><b>RESULTS</b>Compared with group A [(16.1 +/- 3.5)d], MST of skin grafts in group C [(38.3 +/- 7.7) d] and E [(34.9 +/- 7.7) d] were significantly prolonged ( P < 0.01), while no obvious difference was observed between group C and E( P > 0.05), but there was statistically significant difference in MST between group D [(23.7 +/- 2.7) d] and E ( P < 0.05). In addition, clear epithelial structure and infiltration of inflammatory cells were observed in specimens from both groups C and E.</p><p><b>CONCLUSION</b>Both donor derived immature DC and third-party DC loaded with donor's antigens can partly induce donor specific transplantation tolerance.</p>


Subject(s)
Animals , Cyclophosphamide , Pharmacology , Dendritic Cells , Allergy and Immunology , Female , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Inbred Strains , Random Allocation , Skin Transplantation , Allergy and Immunology , Transplantation Tolerance , Allergy and Immunology , Transplantation, Heterologous , Allergy and Immunology
16.
Article in Chinese | WPRIM | ID: wpr-253468

ABSTRACT

<p><b>AIM</b>To search for a new method of left ventricular intubation in rat.</p><p><b>METHODS</b>The wire of percutaneous transluminal coronary angioplasty (PTCA) was put into carotid artery through external carotid artery. Then supported by guide wire, the PE50 tube was advanced into left ventricle.</p><p><b>RESULTS</b>Left ventricular intubation with PTCA guide wire could be performed in 30 rats and successfully repeated in 27 animals.</p><p><b>CONCLUSION</b>The new left ventricular intubation technology in rats is simple and provides a reproducible method.</p>


Subject(s)
Anesthesia , Methods , Animals , Heart Ventricles , Hemodynamics , Physiology , Intubation , Methods , Male , Rats , Rats, Wistar
17.
Chinese Journal of Cardiology ; (12): 833-836, 2006.
Article in Chinese | WPRIM | ID: wpr-238506

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of COX1 and COX2 on angiogenesis and endothelial progenitor cell mobilization in rats with experimental myocardial infarction (MI).</p><p><b>METHODS</b>The rats were randomly divided into 3 groups: MI group, MI plus rofecoxib group and MI plus valeryl salicylate group. At the 7th day after operation, circulating EPCs, plasma VEGF and HIF-1alpha mRNA of ischemic myocardium were measured. At the 28th day post operation, capillary densities were also measured in ischemic myocardium.</p><p><b>RESULT</b>Compared with the MI group and the MI plus valeryl salicylate group, circulating EPCs, plasma VEGF, HIF-1alpha mRNA and capillary densities of ischemic myocardium were all decreased in MI plus rofecoxib group.</p><p><b>CONCLUSION</b>The present study revealed that COX2 play an important role with angiogenesis and endothelial progenitor cell mobilization in rat with experimental MI by modulating expression of VEGF and HIF-1alpha.</p>


Subject(s)
Animals , Cyclooxygenase 2 , Cyclooxygenase Inhibitors , Pharmacology , Endothelial Cells , Cell Biology , Female , Hypoxia-Inducible Factor 1, alpha Subunit , Myocardial Infarction , Drug Therapy , Pathology , Neovascularization, Physiologic , Random Allocation , Rats , Rats, Sprague-Dawley , Stem Cells , Metabolism , Vascular Endothelial Growth Factor A , Blood
18.
Article in Chinese | WPRIM | ID: wpr-686045

ABSTRACT

Background Stromal cell-derived factor-1_?(SDF-1_?)has been demonstrated to be essential for stern cell mobilization/homing.Recent evidence indicates that SDF-1_? has been expressed in injured carotid arter- ies.Besides,high SDF-1_? plasma levels are clinically associated with stable coronary artery disease.Objective To investigate whether SDF 1 involves in mobilization of endothelial progenitor cells(EPC)and reendothelialization after vascular injury.Methods SDF-1_? was detected by RT-PCR and Western blot in carotid arteries of mice at different time points after wire-induced injury.SDF-1_? determination in peripheral blood samples and BM was per- formed by SDF-1_? enzyme-linked immunosorbent assay(ELISA)kit.EPC in peripheral blood collected at different time points after vascular injury were quantified by flow cytornetry.In subgroup,blocking SDF-1 rnonoclonal anti- body was injected,peripheral blood EPC were quantified after vascular injury and reendothelialization of injured ar- teries was determined 14 days later.Results Expression of SDF-1_? was evident at day 1,and peaked at day 3 after arterial injury.A rise in plasmatic concentration of SDF-1_? and a significant reduction of SDF-1_? in bone marrow concentration was noticed at all time points following injury.The amount of circulating EPC was increased shortly after induction of vascular injury and persisted up to 7 days(P

19.
Article in Chinese | WPRIM | ID: wpr-288940

ABSTRACT

<p><b>OBJECTIVE</b>This study was intended to design a kind of resisting part dint of device in order to preventing the base plate break while being subjected to the dint when partial base plate dint concentrates because of the increase of the magnetic attachment to the original movable artificial teeth.</p><p><b>METHODS</b>Ten patients who should increase magnetic attachment was adopted in the study, and we increased a kind of new designed partial cast base plate on the original base plate using laser welding technique, then designed magnetic attachment and artificial teeth.</p><p><b>RESULTS</b>None of ten sufferers appeared the phenomenon of the break of the base plate.</p><p><b>CONCLUSION</b>The application of the laser welding technique can prevent the break of the base plate when partial dint increase because of the increase of the magnetic attachments.</p>


Subject(s)
Dental Soldering , Humans , Lasers , Magnetic Phenomena , Magnetics , Welding
20.
Chinese Journal of Cardiology ; (12): 1021-1025, 2006.
Article in Chinese | WPRIM | ID: wpr-304984

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of sirolimus on differentiation, proliferation, adhesion and migration of endothelial progenitor cells (EPC) in vitro.</p><p><b>METHODS</b>(1) Mononuclear cells (MNC) were isolated from rat bone marrow by Ficoll density gradient centrifugation and cultured on fibronectin-coated culture dishes with or without sirolimus (0.01 - 100 ng/ml) for 12 days. (2) After 8 days cultured, attached cells were treated with sirolimus (0.1 - 200 ng/ml) or vehicle for various time points (12 h, 24 h, 48 h and 96 h). EPC were identified as adherent cells double positive stained for FITC-UEA-I and DiI-acLDL under laser confocal immunofluence microscope. EPC proliferation, migration were assayed with MTT assay and modified Boyden chamber assay respectively.</p><p><b>RESULTS</b>EPC number differentiated from MNC at 12 days was significantly lower in sirolimus treated cells in a dose-dependent manner than that of vehicle-treated cells. Sirolimus also significantly inhibited the proliferative, migratory and adhesive capacity of EPC in a time and dose dependent manner.</p><p><b>CONCLUSION</b>Present results suggested that sirolimus could inhibit EPC differentiation from MNC and reduce the proliferation, migration and adhesion capacities of EPC.</p>


Subject(s)
Animals , Bone Marrow Cells , Cell Differentiation , Cell Movement , Cells, Cultured , Endothelial Cells , Cell Biology , Female , Male , Rats , Rats, Wistar , Sirolimus , Pharmacology , Stem Cells
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