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1.
Article in Chinese | WPRIM | ID: wpr-880173

ABSTRACT

OBJECTIVE@#To investigate the clinical characteristics and risk factors of cytomegalovirus (CMV) infection after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients with severe aplastic anemia (SAA).@*METHODS@#Clinical data from 270 SAA patients with allo-HSCT were retrospectively analyzed, including 108 sib congruence patients and 162 substitute donors (68 unrelated donor congruence patients and 94 related haploid patients). Different pretreatment schemes were selected for different transplantation modes. The HLA-identical sibling and haploid grafts were all bone marrow and peripheral blood stem cells, and the grafts from unrelated donors were peripheral blood stem cells. After granulocyte implantation, blood CMV-DNA was regularly monitored. Flow cytometry was also used to determine the absolute number of CD3@*RESULTS@#CMV infection occurred in 229 of 270 patients with an incidence of 84.8%. Among them, 18 patients developed giant cell disease. Univariate analysis showed that alternative donors (unrelated total and haploid donors), mycophenolate mofetil and acute graft-versus-host disease were statistically significantly associated with CMV infection (P<0.05). Multivariate analysis showed that alternative donors were associated with CMV infection. The recovery of CD3@*CONCLUSION@#After allo-HSCT, substitute donors are more easily to develop CMV infection than full-sibling donors, and the reconstruction of immune function is delayed after transplantation.


Subject(s)
Anemia, Aplastic , Cytomegalovirus Infections , Graft vs Host Disease , Hematopoietic Stem Cell Transplantation , Humans , Retrospective Studies
2.
Article in Chinese | WPRIM | ID: wpr-703585

ABSTRACT

This study aimed to establish a new technology for health insurance reimbursement evidence-based decision-making framework on the basis of EVIDEM. Literature review,focus group discussion and qualitative inter-view were used to construct the preliminary decision-making framework,and expert consultation was adopted to deter-mine the necessity and weight of the criteria. The established evidence-based decision-making framework consists of guidelines of normative universal and contextual aspects. The normative aspect included following criteria, need for intervention (i.e. disease severity, size of affected population, benefit type of technology, unmet needs of reim-bursed technology),comparative outcomes of technology (i.e. comparative effectiveness,comparative safety/tolera-bility,comparative patient-perceived/patient-reported outcomes), and economic consequences of technology (i.e. cost,results of economic evaluation). The contextual aspect reflects the mission and mandate of medical insurance, population priorities and the accessibility,common goal and specific interests, political context, and affordability of medical insurance.

3.
Journal of Experimental Hematology ; (6): 1761-1767, 2017.
Article in Chinese | WPRIM | ID: wpr-278747

ABSTRACT

<p><b>OBJECTIVE</b>To explore the value of CD3CD4T cell count in prediction of viral infections after allogeneic hematopoietic stem cell transplantation(allo-HSCT) in the patients with severe aplastic anemia(SAA).</p><p><b>METHODS</b>A total of 78 SAA patients with allo-HSCT in Guangzhou First People's Hospital from January 2014 to July 2016 were enrolled in this study. The absolute numbers of CD3CD4T cells were measured by flow cytometry at 1,2,3,6, and 12 month after allo-HSCT. According to the cell counts, the patients were divided into 3 groups: i.e. <50/µl (n=120), 50-100/µl(n=48) and >100/µl(n=123)groups. The infection incidences of human cytomegalovirus (HCMV) and Epstein-Barr virus(EBV) within 2 weeks around each time point were compared between different groups. According the counts of CD3CD4T cells at 3 months after-transplant, these patients were divided into 2 groups, i.e.>100/µl (n=30) and ≤100/µl (n=48). The incidences and duration of HCMV and EBV infection, overall survival rate were compared between 2 groups.</p><p><b>RESULTS</b>The incidences of CMV and EBV infection significantly decreased in CD3CD4T cell >100/µl group as compared with <50/µl and 50-100/µl groups. At 3 months after-transplant, there was lower incidence rates of CMV disease, EBV infection, shorter durations of CMV infection and better survival in CD3CD4T cell >100/µl group as compared with ≤100/µl group.</p><p><b>CONCLUSION</b>CD3CD4T cell count is a good predictor for CMV and EBV infection after allo-HSCT in SAA patients. There are low risk of infe-ctions from CMV and EBV when CD3CD4T cell count >100/µl in any time after transplant, which means lower occurrence of CMV and EBV infection and better survival when CD3CD4T cell counts is >100/µl in 3 months after transplant in SAA patients.</p>

4.
Journal of Experimental Hematology ; (6): 1576-1581, 2015.
Article in Chinese | WPRIM | ID: wpr-272558

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of RYBP gene on sensitivity of HL-60 cells to chemotherapy drugs by using RNA interference.</p><p><b>METHODS</b>Plasmid expressing RYBP specific shRNA was constructed and then was used to establish the RYBP knockdown stable HL-60 cell line. Q-PCR and Western blot were used to confirm the efficacy of RYBP gene silencing at mRNA and protein level respectively; then the DNA ladder and Annexin V labeled flow cytometry were used to detect cell apoptosis; CCK-8 was used detect the sensitivity of HL-60 cells to the chemotherapeutic drug cytarabine or daunorubicin.</p><p><b>RESULTS</b>The lentiviral-RYBP-shRNA vector was succesfully and effectively inhibit the expression of RYBP at mRNA and protein in HL-60 cells. It was found that without chemotherapy drug treatment the apoptosis rate of RYBP shRNA group was lower than that of the empty vector control group (NC group). When treated with cytarabine, the apoptosis rate and inhibitive rate of RYBP shRNA group were lower than those of NC group. Besides, when treated with daunorubicin, the apoptosis rate of RYBP shRNA group was lower than that of NC group, while the inhibitive rate had no significant difference.</p><p><b>CONCLUSIONS</b>RYBP gene silencing can inhibitive the apoptosis of HL-60 cells and significantly reduce the sensitivity to cytarabine, but this gene silencing can't affect the sensitivity to daunorubicin.</p>


Subject(s)
Apoptosis , Genetic Vectors , HL-60 Cells , Humans , Intracellular Signaling Peptides and Proteins , Lentivirus , Plasmids , RNA Interference , RNA, Messenger , RNA, Small Interfering
5.
Article in Chinese | WPRIM | ID: wpr-271673

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the skin complications of 16 cases received bone-anchored hearing aid (BAHA) implantations, and the clinical experience for prevention and treatment skin complications following BAHA.</p><p><b>METHODS</b>Retrospective review 16 patients who received BAHA implantation from December 2010 to March 2013, and summarize the complications and treatment.</p><p><b>RESULTS</b>Four patients (1/4) suffered from skin complications. According to Holgers classification, two patients (1/8) had a Holgers Grade 1-2 skin reaction and cured by local application of antibiotics. Two patients (1/8) experienced Holgers Grade 3 skin reaction received revision surgeries for excessive soft tissue growth. One patient replaced the BAHA abutment with a longer 9.0mm one.</p><p><b>CONCLUSIONS</b>Infection and skin overgrowth around the abutment was the common complications of BAHA implantation. Standardize the peri-operative managements, and clean the skin regularly would prevent the skin complication. Additional surgical intervention should be applied timely in the cases of severe complications.</p>


Subject(s)
Adolescent , Adult , Child , Female , Hearing Aids , Humans , Male , Postoperative Complications , Retrospective Studies , Skin Diseases , Young Adult
6.
Article in Chinese | WPRIM | ID: wpr-313586

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the efficacy and satisfaction of bone-anchored hearing aids (BAHA) in patients with outer and middle ear deformities.</p><p><b>METHODS</b>Seven patients with bilateral microtia and aural atresia, and three patients with unilateral microtia and bilateral middle ear malformation were fitted with soft-band BAHA for a few months, followed by receiving unilateral BAHA implantation. Mean pure-tone thresholds and speech audiometry tests results were compared among patients without hearing aid, with soft-band BAHA, and with implanted BAHA. Scores from the BAHA users' questionnaires and Glasgow children's benefit inventory (GCBI) were used to measure patient satisfaction and subjective health benefit.</p><p><b>RESULTS</b>The mean pure-tone thresholds of the patients were (64.8 ± 5.9) dBHL for those without hearing aid, (30.2 ± 3.7) dBHL for those with soft-band BAHA, and (20.3 ± 3.9) dBHL for those with implanted BAHA. The average decline in pure-tone threshold was (36.2 ± 8.0) dBHL for those with soft-band BAHA, and an additional decline of (12.2 ± 3.4) dBHL was achieved with implanted BAHA. The average gains in speech discrimination scores (SDS) were (3.00 ± 1.07)% for those without hearing aids and (89.39 ± 5.83)% for those with implanted BAHA in sound field of 45dBHL. SDS were (57.55 ± 10.30)% for those without hearing aids and (91.19 ± 4.16)% for those with implanted BAHA in sound field of 65dBHL. The average gains in SDS were (88.21 ± 6.86)% and (38.04 ± 7.56)% tested with 45dBHL and 65dBHL respectively. Sound reception thresholds (SRT) without hearing aids were (63.1 ± 5.9) dBHL and (24.7 ± 3.5) dBHL for those with implanted BAHA. The average gains in SRT was (39.6 ± 6.2) dBHL. The BAHA application questionnaire demonstrated excellent patient satisfaction. The general benefit score was 35.59 ± 14.35.</p><p><b>CONCLUSION</b>BAHA remains one of the most reliable methods of auditory rehabilitation and improves quality of life for patients with ear deformities.</p>


Subject(s)
Adolescent , Adult , Auditory Threshold , Child , Ear Auricle , Congenital Abnormalities , Ear, Middle , Congenital Abnormalities , Female , Hearing Aids , Humans , Male , Patient Satisfaction , Treatment Outcome , Young Adult
7.
Article in Chinese | WPRIM | ID: wpr-262464

ABSTRACT

<p><b>OBJECTIVE</b>To establish a model by using zebrafish for the inner ear development and disease research, to explore the possibility of complete dissection of inner ear neuroepithelium in zebrafish by surface preparation of inner ear maculae.</p><p><b>METHODS</b>The inner ear samples of zebra fish were fixed followed by harvesting. After decalcified and dehydrated, the coronal cryosections were made at 3 µm thickness at -23°C using cryostat, the hematoxylin and eosin staining and immunofluorescence and histochemistry staining were performed, the saccules, utricles and lagenaes were successfully obtained by fine dissection. The sensory maculae were mounted on glass and then were stained.</p><p><b>RESULTS</b>After hematoxylin and eosin staining and immunofluorescence and histochemistry staining of cryosections, the sensory maculae of inner ear could be determined by otoliths. On the basis of distinguish of otoliths, the sensory maculae of inner ear could be finely dissected, the overall integrity of the sensory maculae could be preserved completely. After immunofluorescence and histochemistry staining, intact epithelia with strong hair cell bundle staining could be seen.</p><p><b>CONCLUSIONS</b>The neuroepithelium hair cell examination of zebra fish can be entirely attained by surface preparation of inner ear maculae.</p>


Subject(s)
Animals , Cryoultramicrotomy , Ear, Inner , Epithelium , Zebrafish
8.
Article in Chinese | WPRIM | ID: wpr-262427

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this prospective study was to investigate the presence of human papillomavirus (HPV) in tonsillectomy and adenoidectomy specimens from pediatric patients without juvenile-onset recurrent respiratory papillomatosis (JORRP), so as to understand the effect of HPV infection in the upper respiratory tract in children.</p><p><b>METHODS</b>Two hundred and forty-one pediatric patients without known JORRP or other HPV-related diseases undergoing tonsillectomy and/or adenoidectomy for hypertrophy or chronic tonsillitis were enrolled in this prospective study. One hundred and seventy-seven fresh samples of tonsillar tissues and 195 samples of adenoid tissues were collected and then examined for the presence of HPV DNA with the polymerase chain reaction (PCR) technique and typing. Laryngeal papilloma specimens from 17 patients obtained during routine debulking procedures were also analyzed and served as positive controls.</p><p><b>RESULTS</b>All 17 papilloma specimens were positive for HPV DNA and the type was 6 or 11. This result confirmed that the methods used were valid for detecting HPV infection. HPV DNA was detected in 2 of the 177 tonsillar specimens and zero of the 195 adenoid specimens. The two positive samples were confirmed with typing. One was positive for HPV6 and the other for HPV11. Review of the medical records of these two cases confirmed that there were no history of HPV-related diseases. Histologic analysis of their specimens showed lymphoid hyperplasia, no specific changes suggesting HPV infection and no signs of malignancy. The HPV infection rate in upper respiratory tract was 0.8% (2/241).</p><p><b>CONCLUSION</b>There is HPV infection in upper respiratory tract in Chinese children without JORRP, but maybe is not sufficient for the formation of JORRP.</p>


Subject(s)
Child , Child, Preschool , DNA, Viral , Female , Humans , Infant , Male , Palatine Tonsil , Virology , Papillomaviridae , Papillomavirus Infections , Diagnosis , Virology , Prospective Studies , Respiratory System , Virology , Respiratory Tract Infections , Tonsillectomy
9.
Chinese Medical Journal ; (24): 301-304, 2010.
Article in English | WPRIM | ID: wpr-314594

ABSTRACT

<p><b>BACKGROUND</b>The main risk factors for postoperative failure in tympanoplasties are large perforations that are difficult to repair, annular perforations, and a tympanic membrane (TM) with extensive granular myringitis that require middle ear exploration and mastoidectomy. The aim of this study was to investigate a novel technique of perichondrium/cartilage composite graft for repairing the large TM perforation in the patient of otitis media.</p><p><b>METHODS</b>Retrospective chart reviews were conducted for 102 patients with large tympanic membrane perforations, who had undergone tympanoplasty from August 2005 to August 2008. Tympanoplasty or tympanomastoidectomy using a perichondrium/cartilage composite graft was analyzed. The tragal or conchal perichondrium/cartilage was used to replace the tympanic membrane in patients.</p><p><b>RESULTS</b>Patients aged from 13 to 67 years were followed up in average for 24 months (10 - 36 months). Seventy-four ears (72.61%) were used the tragal perichondrium/cartilage as graft material and 27 ears (27.39%) were used the conchal perichondrium/cartilage. Graft take was successful in all patients. Postoperative complications such as wound infection, hematoma, or sensorineural hearing loss were not identified. Nine patients (8.82%) had the partial ossicular replacement prosthesis, 14 patients (13.72%) using the autologous curved incus and 79 patients (77.45%) without prosthesis. Successful closure occurred in 92% of the ears. A total of 85.8% patients achieved a postoperative hearing improvement.</p><p><b>CONCLUSIONS</b>The graft underlay tympanoplasty using perichondrium/cartilage composite is effective for the majority of patients with large perforation. The hearing was improved even if the mastoidectomy was required in the patients with otitis media with extensive granulation.</p>


Subject(s)
Adolescent , Adult , Aged , Cartilage , Transplantation , Female , Hearing Loss , General Surgery , Humans , Male , Middle Aged , Otitis Media , Retrospective Studies , Treatment Outcome , Tympanic Membrane Perforation , General Surgery , Tympanoplasty , Methods , Young Adult
10.
Article in Chinese | WPRIM | ID: wpr-268671

ABSTRACT

<p><b>OBJECTIVE</b>To compare the adipogenic differentiation capacity of dedifferentiated adipocytes cells (DA) and adipose-derived stem cells (ASCs) in vivo, so as to select good adipogenic seed cells for tissue engineering.</p><p><b>METHODS</b>Mature adipocytes and ASCs were isolated by means of enzymatic digestion from the liposuction aspirate. Then the DA cells were acquired by ceiling adherent culture of mature adipocytes and the 3rd passage cells were used. The DA cells and ASCs were cultured with fibrin glue in vitro respectively. The compatibility of scaffold with cells was detected by microscopy and scanning electron microscopy. The scaffold-cell composite was also labeled by DiI. The composite was injected subcutaneously on the nude mice back, respectively (DA-FG group, n = 8; ASCs-FG group, n = 8; sham FG group, n = 8). 8 weeks after implantation, the newly formed tissue was taken out for general observation and histologic study.</p><p><b>RESULTS</b>Mature adipocytes were transferred to DA cells with spindle shape, like fibroblast. The ASCs were also spindle. Three days after culture of cell-scaffold composite in vitro, the cells grew well. 8 weeks after implantation, the newly formed tissue was found under the skin both in DA-FG and ASCs-FG groups, but not in sham FG group. The newly formed tissue was mature fat tissue and originated from the seed cells. The average wet weight of the new-formed tissue was higher in DA-FG group than that in ASCs-FG group. The average fibrosis ratio was lower in DA-FG than in ASCs-FG group.</p><p><b>CONCLUSIONS</b>The tissue-engineered adipose tissue can be achieved with DA cells and ASCs as seed cells. Compared with ASCs, the new-formed fat tissue with DA has a higher wet weight and lower fibrosis ratio.</p>


Subject(s)
Adipocytes , Cell Biology , Adipose Tissue , Cell Biology , Adult , Animals , Cell Differentiation , Cells, Cultured , Female , Humans , Mice , Mice, Nude , Stem Cells , Cell Biology , Tissue Engineering
11.
Article in Chinese | WPRIM | ID: wpr-339207

ABSTRACT

<p><b>OBJECTIVE</b>To explore the feasibility of directionally inducing human adipose-derived mesenchymal stem cells (hAD-MSCs) towards inner ear hair cells.</p><p><b>METHODS</b>Mesenchymal stem cells from human adipose tissue were isolated, purified and cultured in vitro. hAD-MSCs were induced to neural stem/progenitor-like cell, and then co-cultured with embryonic chick otic vesicle cells. Processed hAD-MSCs were tested by immunostaining to ascertain whether they expressed characteristic hair cell markers.</p><p><b>RESULTS</b>Morphologically, hAD-MSCs were induced to differentiate into neural stem/progenitor cells and expressed specific neural markers. After being co-cultured with embryonic chick otic vesicle cells, hAD-MSCs expressed specific surface markers of inner ear hair cells.</p><p><b>CONCLUSIONS</b>hAD-MSCs can be directionally induced to differentiate towards hair cell-like cells in vitro.</p>


Subject(s)
Adipose Tissue , Cell Biology , Animals , Cell Culture Techniques , Methods , Cell Differentiation , Cells, Cultured , Chick Embryo , Coculture Techniques , Hair Cells, Auditory, Inner , Cell Biology , Humans , Mesenchymal Stem Cells , Cell Biology
12.
Article in Chinese | WPRIM | ID: wpr-245876

ABSTRACT

<p><b>OBJECTIVE</b>To study the long-term effect of cochlear ablation on cochlear nucleus (CN).</p><p><b>METHODS</b>A total of 40 BALB/c adult mice were used in the present study. Mice were divided into experimental group and control group at random. In experimental group, the cochlea was destroyed with stainless steel needle under the anatomy microscopy. Mice were tested for ABR thresholds at second day and 4 months after operation to assess hearing sensitivity. Animals were allowed to survive for four months. Histological sections of the cochlear nucleus were evaluated with serial sections stained with Nissl staining and silver staining alternatively. Morphological change in anteroventral cochlear nucleus (AVCN), posteroventral cochlear nucleus (PVCN), dorsal cochlear nucleus (DCN) and octopus cell area (OCA) was observed evaluated four month later after the cochlear ablation.</p><p><b>RESULTS</b>In the experimental group, after operation, early components of ABR couldn't be recognized. The volume of CN was decreased dramatically and the cells were less comparing to the control group. The volume of AVCN after cochlear ablation showed a decline to 22% less than the control group, and the number of neurons also dropped by 25%. In PVCN, the decreased volume by 40% with significant neuronal loss of 47% was observed four month later after the cochlear ablation. And in DCN, the volume shrinking to 24% and the loss of neurons reached to 39%. The most significant change was observed in OCA (octopus cell area), with the neuron numbers, the area and the volume of OCA decreased by 60%, 19%, and 47% respectively. There was a statistically significant difference (P<0.05, Mann-Whitney U test) in the morphological changes between the two groups.</p><p><b>CONCLUSION</b>Neuronal degeneration was observed in cochlear nucleus after four months cochlear ablation.</p>


Subject(s)
Animals , Auditory Pathways , Pathology , Cochlea , Pathology , Cochlear Nucleus , Pathology , Female , Male , Mice , Mice, Inbred BALB C , Nerve Degeneration
13.
Article in Chinese | WPRIM | ID: wpr-233730

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the dedifferentiation of mature adipocytes and the possibility of adipose tissue engineering using dedifferentiated adipocytes.</p><p><b>METHODS</b>Human adipose tissue was harvested from healthy women undergoing abdominal liposuction procedures, and mature adipocytes were isolated with enzymatic digestion and cultured by ceiling adherent culture method, using the third-passage cells for subsequent experiment. The cells were cultured in adipogenic, chondrogenic or osteogenic media, and Oil red-O staining, Alcian blue staining and Alizarin red staining were used for dedifferentiation identification. The third-passage cells labeled with DiI were mixed with fibrin glue and injected subcutaneously on one side of the nude mouse back (n=6), and fibrin glue solution without cells as control was injected on the other side (n=6). After 8 weeks of cell implantation, the specimens were harvested for general observation and histological, Oil red-O staining and fluorescent microscope analyses.</p><p><b>RESULTS</b>Mature adipocytes were round, unilocular cells. After ceiling adherent culture, the adipocytes underwent morphological changes into fibroblast-like cells indicating their dedifferentiation. The dedifferentiated adipocytes were induced for adipogenic, chondrogenic and osteogenic differentiation in specified media. Eight weeks after the cell injection in nude mice, newly formed tissue occurred which was identified as mature adipose tissue. The implants without cells were completely absorbed in the control group.</p><p><b>CONCLUSION</b>Mature adipocytes can dedifferentiate in vitro culture, and the dedifferentiated adipocytes are capable of differentiating into adipogenic, chondrogenic and osteogenic lineages. Adipose tissue engineering can be achieved in vivo using the dedifferentiated adipocytes as the seed cells.</p>


Subject(s)
Adipocytes , Cell Biology , Adipogenesis , Adult , Animals , Cell Dedifferentiation , Chondrogenesis , Female , Humans , Mice , Mice, Nude , Microscopy, Fluorescence , Osteogenesis , Time Factors , Tissue Engineering , Methods
14.
Article in Chinese | WPRIM | ID: wpr-233692

ABSTRACT

<p><b>OBJECTIVE</b>To detect the expressions of miR-122 and miR-224 in hepatocellular carcinoma (HCC) by real-time fluorescence quantitative RT-PCR and investigate the significance of miRNAs in early diagnosis of HCC.</p><p><b>METHOD</b>2(-Delta Delta CT) method was used for quantitative analysis of the expression pattern of miR-122 and miR-224 in 35 HCC and adjacent normal tissues. All the quantitative results were confirmed by Northern blotting.</p><p><b>RESULTS</b>Compared with adjacent normal tissues, the HCC tissues showed significant miR-122 down-regulation (P<0.01) and miR-224 over-expression (P<0.001).</p><p><b>CONCLUSIONS</b>HCC has obvious alteration in the expression patterns of miR-122 and miR-224, and real-time fluorescence quantitative RT-PCR provide a new means for early, efficient, and accurate diagnosis of HCC.</p>


Subject(s)
Adult , Aged , Base Sequence , Blotting, Northern , Carcinoma, Hepatocellular , Diagnosis , Genetics , Female , Gene Expression Regulation, Neoplastic , Humans , Liver Neoplasms , Diagnosis , Genetics , Male , MicroRNAs , Genetics , Middle Aged , Reverse Transcriptase Polymerase Chain Reaction , Spectrometry, Fluorescence , Time Factors
15.
Article in Chinese | WPRIM | ID: wpr-339265

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the clinical features and treatment protocols of otogenic intracranial complications in Peking Union Medical College Hospital.</p><p><b>METHODS</b>Retrospective study of 14 patients (10 males and 4 females, aged between 12 - 62 years, mean age 32.1 years) hospitalized from 1982 - 2006. Twelve cases were otitis media (OM) with cholesteatoma, the other 2 cases were non-cholesteatomatous OM. All the otogenic intracranial complications located at the same sides as otologic disorders. Brain abscess was the most common type of otogenic complications and Proteus was the most common microorganism detected. Suppurative ear discharge, headache, high fever and nausea with vomiting were the most common clinical manifestations with very high incidences. All the patients received combined protocols of mastoid surgeries and antibiotics treatment.</p><p><b>RESULTS</b>All the 14 patients recovered clinically. For patients discharged before 1987, there were 4 patients followed up for 22.5 - 24.4 years with a mean time of 23.8 without recurrence, 1 patient died of cardiovascular disease 19.2 years later after discharge, 4 patients lost follow-up. For the 5 patients discharged after 1997, brain abscess recurred in one patient with pseudo-recovery after 24 days and he fully recovered after re-hospitalization and treatment. All the five patients were followed up for 1.5 years to 10.6 years with a mean time of 6.5 years without recurrence.</p><p><b>CONCLUSIONS</b>Youngsters and males seemed to be more vulnerable. Brain abscess was the most common intracranial complication and Proteus was the most common pyogenic microorganism. Combination of mastoid surgery and antibiotics were essential for effectively controlling the intracranial complications and improving the recovery. CT and MRI were essential for correct diagnosis bedtimes and MRI seemed to have a better performance.</p>


Subject(s)
Adolescent , Adult , Brain Abscess , Child , Ear Diseases , Female , Humans , Male , Middle Aged , Otitis Media , Retrospective Studies , Young Adult
16.
Article in Chinese | WPRIM | ID: wpr-343974

ABSTRACT

Application of traditional Chinese medicine and drugs in cardiac surgery have won great progress in recent 10 years, mainly presented in aspects of the treatment of coronary heart disease, myocardium protection, cardiopulmonary bypass and pulmonary hypertension.


Subject(s)
Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Humans , Medicine, Chinese Traditional , Methods , Postoperative Complications , Drug Therapy , Quality of Life , Thoracic Surgery
17.
Article in Chinese | WPRIM | ID: wpr-331984

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effects of Shenfu Injection (SI) on immune function in patients during perioperative cardiopulmonary bypass (CPB).</p><p><b>METHODS</b>Ninety patients scheduled for CPB were randomly divided into 3 groups, the group A treated with SI before CPB, the group B treated with SI after CPB and the group C (the control group), 30 cases in each group. Counts of leukocyte, neutrophil, lymphocyte and T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) and serum levels of IgG, IgA, IgM, C3 and C4 were investigated at different time-points, i.e. one day before CPB, immediately after anesthesia and finishing CPB, and the 1st, 3rd and 8th post-operational days.</p><p><b>RESULTS</b>In all the three groups, counts of leukocyte and neutrophil rose immediately after CPB and the high level maintained on the 1st and 3rd post operational days, it somewhat lowered on the 8th day but still higher than that before operation, showing insignificant difference among the 3 groups. As for the other indexes, they declined obviously and immediately after CPB and began to restore on the 8th day to a certain extent, and the restoration were better in the group A and B, as the changes were milder.</p><p><b>CONCLUSION</b>SI could improve the immune function and reduce the risk of post operational infection in patients during perioperative CPB.</p>


Subject(s)
Adjuvants, Immunologic , Therapeutic Uses , Adult , Aged , Cardiopulmonary Bypass , Drugs, Chinese Herbal , Therapeutic Uses , Female , Heart Diseases , Allergy and Immunology , General Surgery , Humans , Injections, Intravenous , Intraoperative Period , Male , Middle Aged , Phytotherapy , Postoperative Complications , T-Lymphocyte Subsets
18.
Article in Chinese | WPRIM | ID: wpr-315568

ABSTRACT

<p><b>OBJECTIVE</b>The ossified cochlea was no longer an absolute contra-indication to cochlear implantation but it was still a challenge even for the experienced otologist. To report the condition of cochlear implantation and the hearing results of 31 cases of cochlear ossification.</p><p><b>METHODS</b>Retrospective analysis of cochlear implantation of 720 cases of cochlear implantation from May 1995 to July 2005 in Peking Union Medical College Hospital.</p><p><b>RESULTS</b>Thirty-one cochlear ossification patients was found from all the 720 surgeries which included 27 mild cases and 4 severe ones, 14 males, 17 females. And the age of operation was 1. 4 - 59. 0 years old , mean age was 13. 2 years old. All of them had cochlear implanted through mastoid-facial recess. There was no complications during or post the operations. And the hearing threshold was about 40 dB in the sound field, the results were similar to those without cochlear ossification.</p><p><b>CONCLUSIONS</b>Although it was difficult of cochlear implantation in cochlear ossification patients, but in most cases especially mild ones all the electrodes could be implanted after clearing the ossified tissue, and had few damage to the electrodes. Now it was possible to practice in the partial cochlea ossification.</p>


Subject(s)
Adolescent , Adult , Child , Child, Preschool , Cochlear Diseases , General Surgery , Cochlear Implantation , Cochlear Implants , Female , Humans , Infant , Male , Middle Aged , Ossification, Heterotopic , General Surgery , Retrospective Studies , Young Adult
19.
Article in Chinese | WPRIM | ID: wpr-315567

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the indications, surgical techniques and complications of cochlear implantation in patients with otitis media-related diseases.</p><p><b>METHODS</b>Retrospective study of the data collected from patients receiving cochlear implantation. Totally 866 cases of cochlear implantation were performed in Peking Union Medical College Hospital from May 1995 to February 2006. Among which, 41 patients with otitis media-related diseases were grouped into 5 types: chronic secretory otitis media (13 cases), silent (subclinical) otitis media (18 cases), dry eardrum perforation (1 case), bilateral cholesteatoma of middle ear (2 cases) and middle ear granuloma (7 cases). Seven cases were accompanied with deformities of middle ear and (or) inner ear. Pedicled aponeurosis of occipitofrontalis muscle was transplanted to cover and protect the inserted electrodes and facial nerve in a patient with bilateral cholesteatoma after radical mastoidectomy.</p><p><b>RESULTS</b>All the 41 patients with otitis media-related diseases were successfully implanted in one stage or staged operations and followed up uneventfully for 5 months to 6 years and 11 months. All implant devices had worked normally and all patients had performed well.</p><p><b>CONCLUSIONS</b>Patients with chronic secretory otitis media, silent (subclinical) otitis media, middle ear granuloma or dry ear-drum perforation could be operated in one stage or staged procedures safely and effectively. Patients with bilateral cholesteatoma could be implanted after radical removal of related lesions. Pedicled aponeurosis of occipitofrontalis muscle could be transplanted in cases of mastoid bowl to cover and protect the inserted electrodes and the exposed facial nerve and with easy access to observe the mastoid cavity. Active suppurative otitis media was contraindicated for cochlear implantation. Long-term following-up was essential for better evaluation of the outcomes of cochlear implantation in patients with otitis media-related diseases.</p>


Subject(s)
Adolescent , Adult , Child , Child, Preschool , Cochlear Implantation , Cochlear Implants , Female , Humans , Infant , Male , Otitis Media , Classification , General Surgery , Retrospective Studies , Treatment Outcome , Young Adult
20.
Article in Chinese | WPRIM | ID: wpr-232862

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the risk factors of intrauterine hepatitis B virus (HBV) infection and the impact of HBV DNA on the infection.</p><p><b>METHODS</b>The serum levels of HBsAg, HbsAb, HBeAg, HBeAb, HBcAb and HBV DNA were determined in blood samples from 230 HBsAg-positive pregnant women and their newborns by enzyme-linked immunosorbent assay (ELISA) and fluorescence quantitative PCR (FQ-PCR), respectively. The newborns acquiring HBV infection via intrauterine transmission were selected as the case group and others as the control group. The risk factors for intrauterine HBV infection were analyzed by non-conditional logistic regression model.</p><p><b>RESULTS</b>Six infants were found to be HBsAg-positive, and 18 HBV DNA-positive, and 3 of them were positive for both HBsAg and HBV DNA. The rate of intrauterine HBV infection was 9.6% (22/230). The grade of HBV DNA level was identified as the only risk factor of intrauterine HBV infection by non-conditional logistic regression model, with odds ratio (OR) of 1.57 (95% confidence interval 1.12-2.21). Of the 119 pregnant women positive for HBV DNA, 18 were diagnosed as having intrauterine HBV infection, and the likeliness of the infection significantly increased for a maternal serum HBV DNA level > or =10(7) copies/ml (chi(2)=7.92, P<0.05).</p><p><b>CONCLUSION</b>The grade of serum HBV DNA level is the predominant risk factor for intrauterine HBV infection in pregnant women, and for those with serum HBV DNA lever > or =10(7) copies/ml, the chance for intrauterine HBV infection can be significantly increased.</p>


Subject(s)
DNA, Viral , Blood , Genetics , Enzyme-Linked Immunosorbent Assay , Female , Hepatitis B , Virology , Hepatitis B Antibodies , Blood , Hepatitis B Surface Antigens , Blood , Hepatitis B virus , Genetics , Allergy and Immunology , Humans , Infant, Newborn , Infectious Disease Transmission, Vertical , Logistic Models , Odds Ratio , Polymerase Chain Reaction , Pregnancy , Pregnancy Complications, Infectious , Blood , Virology , Risk Factors
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