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1.
Article in Chinese | WPRIM | ID: wpr-817646

ABSTRACT

@# Emergencies refer to those events that cause serious social harm, including natural disasters and public health events, and require emergency response. Medical rescue team is the main emergency rescue team. While carrying out the rescue mission, they are under great pressure both physically and mentally due to the unadaptability of the rescue environment, the lack of protective materials, overwork and other reasons, often resulting in fear, tension, anxiety, pessimism, self-blame and even acute stress disorder. Without timely and effective psychological support, long-term psychological problems such as post-traumatic stress disorder will remain after the event. Comprehensive psychological support includes psychological measurement of the whole rescue process, team formation before rescue and detailed psychological support intervention training, self-relaxation during rescue, basic life and safety guarantee, drug treatment, online psychological assistance, withdrawal of stressors after rescue and lifestyle reconstruction.

2.
Acta Physiologica Sinica ; (6): 488-498, 2020.
Article in Chinese | WPRIM | ID: wpr-827038

ABSTRACT

Primary cilium, widely distributed in mammalian central nervous system, is an important extracellular organelle of cells. The primary cilia contain a variety of ion channels, G-protein coupled receptors and different kinds of kinases, which indicates that primary cilia can detect extracellular signals and transduce them into cells to regulate various cellular and physiological processes. In humans, mutations of genes related to structure and function of primary cilia always cause various monogenetic diseases. Moreover, a series of neuropsychiatric diseases and neurodevelopmental dysplasia are caused by abnormal functions of G-protein coupled receptors, kinases and ion channels in primary cilia. This article reviews recent research progress on the role of primary cilia in related neurological diseases.


Subject(s)
Animals , Central Nervous System , Cilia , Humans , Ion Channels , Nervous System Diseases , Receptors, G-Protein-Coupled
3.
Article in Chinese | WPRIM | ID: wpr-772577

ABSTRACT

OBJECTIVE@#To study the neuroprotective effect of icariin on spinal cord injury in rats.@*METHODS@#A total 108 SPF male 3-month-old SD rats were divided into experimental group, control group and sham operation group according to the random number table. There were 36 rats in each group. In the control group and the experimental group, the modified Allen's method was used to make the spinal cord injury model. In the sham operation group, only the lamina was cut without damaging the spinal cord. Immediately after operation, the experimental group was given intragastric administration of icariin(100 mg/kg), the control group and sham operation group were given an equal amount of normal saline by gavage, twice a day. BBB score was used to assess the motor function of rats on 1, 2, 3 days after operation. At 72 h after operation, the activity of myeloperoxidase (MPO) was measured by spectrophotometry. Tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) levels was detected by enzyme-linked immunosorbent assay(ELISA). MPO, TNF-α, IL-1β expression were detected by immunohistochemical staining. Malondialdehyde (MDA) content was detected by thiobarbituric acid method. Superoxide dismutase (SOD) activity was measured by xanthine oxidase method. TUNEL staining was used to detect the apoptosis and apoptosis index(AI) was calculated. The histopathological changes of the spinal cord were observed under a light microscope and the histopathological score was performed using Sirin score method.@*RESULTS@#BBB score in the control group and the experimental group was significantly lower than that in the sham operation group at each postoperative time point(<0.05). BBB score in the experimental group was significantly higher than that in the control group at 2 and 3 days after operation (<0.05). At 72 h after operation, the MPO activity and the levels of TNF-α, IL-1β in the control group and experimental group were significantly higher than in the sham operation group (<0.05), and the experimental group was obviously higher than control group(<0.05). The expressions of MPO, TNF-α, IL-1β in the control group and experimental group were significantly higher than in the sham operation group (<0.05), and the experimental group was significantly lower than of the control group (<0.05). MDA content in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (<0.05). SOD activity in the control group and the experimental group was significantly lower than that in the sham operation group, and the experimental group was significantly higher than that in the control group (<0.05). The AI in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (<0.05). The histopathological score in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (<0.05).@*CONCLUSIONS@#Icariin can inhibit inflammation, lipid peroxidation and apoptosis after spinal cord injury, reduce histopathological damage of spinal cord, improve the motor function, effectively protect spinal cord tissue, and has an obvious neuroprotective effect.


Subject(s)
Animals , Flavonoids , Male , Neuroprotective Agents , Rats , Rats, Sprague-Dawley , Spinal Cord , Spinal Cord Injuries , Tumor Necrosis Factor-alpha
4.
Article in Chinese | WPRIM | ID: wpr-691251

ABSTRACT

<p><b>OBJECTIVE</b>To assess the effects of intragastric administration of icariin on lipid peroxidation after spinal cord injury in rats.</p><p><b>METHODS</b>Seventy-two healthy adult male SD rats were randomized equally into icariin group, control group and sham-operated group. In the control and icariin groups, spinal cord injury was induced using modified Allen's method, and the rats in the sham-operated group underwent laminotomy without damaging the spinal cord. Immediately after the surgery, the rats in icariin group were subjected to intragastric administration of icariin (100 mg/kg), and those in the control and sham-operated groups received an equal volume of saline in the same manner once a day. At 24 h after the operation, malondialdehyde (MDA) content was detected using thiobarbituric acid method, superoxide dismutase (SOD) activity was measured with xanthine oxidase method, and the water content in the spinal cord was measured using dry-wet weight method. At 48 h after the operation, the ultrastructure of the spinal cord was observed with transmission electron microscopy and scored using Kaptanoglu scoring method. The motor function of the rats was assessed using BBB scoring at 7, 14, 21 and 28 days after the operation.</p><p><b>RESULTS</b>At 24 h after the operation, MDA content was significantly higher in the control group and icariin group than in the sham-operated group, and was significantly lower in icariin group than in the control group (P<0.05); SOD activity was significantly higher in icariin group than in the control group, and was both significantly lower than that in the sham-operated group (P<0.05). At 48 h after operation, the water content and ultrastructure score of the spinal cord were the highest in sham-operated group (P<0.05), and were significantly lower in icariin group than in the control group (P<0.05). At all the time points of measurement, the BBB scores were significantly lower in the control and icariin groups than in the sham-operated group (P<0.05), and were significantly higher in icariin group than in the control group (P<0.05).</p><p><b>CONCLUSION</b>Icariin can significantly reduce MDA content, increase SOD activity, and ameliorate lipid peroxidation, spinal cord edema, and histopathological damage of the spinal cord to improve motor function of rats with spinal cord injury.</p>

5.
Chinese Journal of Immunology ; (12): 25-30, 2018.
Article in Chinese | WPRIM | ID: wpr-702667

ABSTRACT

Objective:To analyze the effects of BANCR on proliferation,apoptosis,invasion and angiogenesis in human hepatocarcinoma cell line HepG2.Methods:The expression of BANCR was detected by quantitative real-time reverse transcription PCR (qRT-PCR).BANCR siRNA and Scramble was respectively transfected into human hepatocarcinoma cell line HepG2.Cell proliferation was detected by CCK-8.Flow cytometry was performed to analyze the apoptosis.Transwell assay was used to test the invasion.Angiogenesis was analyse by tube formation assay.Western blot was executed to check the expression of proliferating cell nuclear antigen (PCNA),caspase-3,matrix metalloprotein 9 (MMP-9),vascular endothelial growth factor(VEGF),acidic fibroblast growth factor (bFGF)and interferon-γ (IFN-γ).Results:The expression of BANCR in HepG2 was higher than L02 (P<0.05).Compared with control group,the cell proliferation folds in BANCR siRNA was largely decreased.Besides,BANCR siRNA group had a higher apoptosis rate and less invasive cells (P<0.05).Western blot showed that the expression level of caspase-3 and IFN-γwas obviously enhanced in BANCR siRNA group,and the expression of PCNA,MMP-9,Fn,Vimentin,VEGF and bFGF was distinctly surpressed in BANCR siRNA group compared to control group (P < 0.05).Conclusion:siRNA interference of BANCR promotes apoptosis and represses proliferation,invasion and angiogenesis in human hepatocarcinoma cell line HepG2.

6.
Article in Chinese | WPRIM | ID: wpr-698450

ABSTRACT

BACKGROUND: Mesenchymal stem cells are derived from a variety of tissues, such as bone marrow, pulp, placenta, umbilical cord and adipose tissue. Mesenchymal stem cells from deciduous pulp have strong stemness and biological activity, no rejection, and strong immunoregulation, which are one of excellent cell sources for biotherapy. It is easy and suitable for large-scale production of mesenchymal stem cells from deciduous pulp, thereby laying a good foundation for the industrialization of dental pulp stem cells. OBJECTIVE: To investigate the effect of primary tooth root resorption on the isolation and expansion of dental pulp stem cells, in order to further determine the proper period for tooth extraction for pulp stem cell isolation. METHODS: Totally 173 primary teeth from 173 pupils aged 7-9 years were extracted for the isolation and expansion of dental pulp stem cells. Before tooth extraction, we took X-ray periapical film or orthopantomography of the primary teeth, in accordance with the World Health Organization (WHO) professional inspection standard. Root resorption in primary teeth could be divided into five kinds: root resorption 1/3, root resorption 1/2, root resorption 2/3, complete root resorption, and natural loss of primary teeth. Collected teeth after tooth extraction were placed into a medium within 7 seconds, and stored at in a refrigerator of 2-4 ℃. Then, the teeth were sent to the Oral Stem Cell Bank in Beijing within 24 hours by a professional cold-chain logistics for the isolation, expansion and preservation of dental pulp stem cells. Statistical analysis of the test results was performed. RESULTS AND CONCLUSION: For 32 primary teeth with root resorption 1/3, dental pulp stem cells were successfully extracted from 30 teeth, with a success rate of 94%, and ectopic eruption of permanent teeth was found in 12 cases, with an average eruption time of (2.19±0.18) months. For 35 primary teeth with root resorption 1/2, dental pulp stem cells were successfully extracted from 32 teeth, with a success rate of 92%, and ectopic eruption of permanent teeth was found in 11 cases, with an average eruption time of (1.89±0.13) months. For 59 primary teeth with root resorption 2/3, dental pulp stem cells were successfully extracted from 54 teeth, with a success rate of 92%, and ectopic eruption of permanent teeth was found in 8 cases, with an average eruption time of (1.42±0.12) months. For 37 primary teeth with complete root resorption (the bottom of the pulp was intact), dental pulp stem cells were successfully extracted from 34 teeth, with a success rate of 92%, and ectopic eruption of permanent teeth was found in 2 cases, with an average eruption time of (1.03±0.15) months. For 10 naturally exfoliated primary teeth, dental pulp stem cells were not extracted, and ectopic eruption of permanent teeth was found in 4 cases, with an average eruption time of (0.65±0.23) months. To conclude, the primary teeth naturally exfoliated have no dental pulp with no stem cells; the success rate of extraction is relatively high in primary teeth that have mobility I-II, root resorption 2/3 or complete root resorption but with the complete bottom of the pulp. Moreover, it has no effect on permanent tooth eruption, and it is the best time for collection of primary teeth.

7.
National Journal of Andrology ; (12): 409-413, 2018.
Article in Chinese | WPRIM | ID: wpr-689742

ABSTRACT

<p><b>Objective</b>Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common genetic renal diseases, which may cause oligoasthenospermia and azoospermia and result in male infertility. This study aimed to analyze the outcomes of preimplantation genetic diagnosis (PGD) in male patients with ADPKD-induced infertility.</p><p><b>METHODS</b>We retrospectively analyzed the clinical data on 7 male patients with ADPKD-induced infertility undergoing PGD from April 2015 to February 2017, including 6 cases of oligoasthenospermia and 1 case of obstructive azoospermia, all with the PKD1 gene heterozygous mutations. Following intracytoplasmic sperm injection (ICSI), we performed blastomere biopsy after 5 or 6 days of embryo culture and subjected the blastomeres to Sureplex whole-genome amplification, followed by haplotype linkage analysis, Sanger sequencing, array-based comparative genomic hybridization to assess the chromosomal ploidy of the unaffected embryos, and identification of the unaffected euploid embryos for transfer.</p><p><b>RESULTS</b>One PGD cycle was completed for each of the 7 patients. Totally, 26 blastocysts were developed, of which 12 were unaffected and diploid. Clinical pregnancies were achieved in 6 cases following 7 cycles of frozen embryo transplantation, which included 5 live births and 1 spontaneous abortion.</p><p><b>CONCLUSIONS</b>For males with ADPKD-induced infertility, PGD may contribute to high rates of clinical pregnancy and live birth and prevent ADPKD in the offspring as well. This finding is also meaningful for the ADPKD patients with normal fertility.</p>


Subject(s)
Abortion, Spontaneous , Genetics , Biopsy , Blastocyst , Comparative Genomic Hybridization , Embryo Transfer , Female , Humans , Infertility, Male , Genetics , Male , Mutation , Polycystic Kidney, Autosomal Dominant , Diagnosis , Genetics , Pregnancy , Pregnancy Outcome , Preimplantation Diagnosis , Retrospective Studies , Sperm Injections, Intracytoplasmic
8.
National Journal of Andrology ; (12): 137-141, 2017.
Article in Chinese | WPRIM | ID: wpr-812797

ABSTRACT

Objective@#To investigate the correlation of the single nucleotide polymorphism (SNP) rs4880 of the superoxide dismutase 2 (SOD2) gene with the risk of male infertility.@*METHODS@#This casecontrol study included 519 male patients with idiopathic infertility (aged 19-40 [28.93±4.93] years) in the case group and 338 fertile men (aged 19-40 [28.40±4.25] years) in the control group. We collected the clinical data, genotyped the SNP rs4880 of the SOD2 gene by Sequenom Mass Array, and analyzed the association of different genotypes with male infertility using the logistic regression model.@*RESULTS@#Statically significant differences were observed between the case and control groups in the level of folliclestimulating hormone (FSH) ([4.72±2.51] vs [15.65±17.24] U/L, P< 0.01), the percentage of progressively mobile sperm ([9.12±13.5] vs [41.95±9.03]%, P< 0.01), and sperm concentration ([12.95±24.38] vs [72.88±45.60] ×106/ml, P< 0.01), but not in other parameters. No correlation was found between male infertility and the heterozygous genotype TC (OR = 0.90, 95% CI: 0.65-1.25, P = 0.516) or the homozygous genotype CC (OR=1.49, 95% CI: 0.38-5.81, P = 0.566) as compared with the wild genotype TT, and similar results were obtained in the analysis of the subgroups.@*CONCLUSIONS@#The SNP rs4880 of the SOD2 gene was not correlated with male infertility, which, however, is to be supported by further studies with larger samples from more areas.


Subject(s)
Adult , Case-Control Studies , Follicle Stimulating Hormone , Blood , Genetic Predisposition to Disease , Genotype , Heterozygote , Humans , Infertility, Male , Genetics , Logistic Models , Male , Nucleotides , Genetics , Polymorphism, Single Nucleotide , Sperm Motility , Superoxide Dismutase , Genetics , Young Adult
9.
National Journal of Andrology ; (12): 38-43, 2015.
Article in Chinese | WPRIM | ID: wpr-319545

ABSTRACT

<p><b>OBJECTIVE</b>To summarize the features and treatment of male infertility induced by autosomal dominant polycystic kidney disease (ADPKD), and compare the outcomes of intracytoplasmic sperm injection (ICSI) for infertile men with ADPKD and those with congenital bilateral absence of vas deferens (CBAVD).</p><p><b>METHODS</b>We retrospectively analyzed 21 cases of ADPKD-induced infertility, 15 treated by ICSI (group A), and another 164 cases of strictly matched CBAVD-induced infertility (group B). We compared the two groups in the couples' age, the number of ICSI oocytes, and the rates of fertilization, transferrable embryos, good embryos, embryos implanted, clinical pregnancy, biochemical pregnancy, early abortion, singleton and twins in the first cycle.</p><p><b>RESULTS</b>After 28 cycles of ICSI, 10 of the 15 ADPKD-induced infertility patients achieved clinical pregnancy, including 7 cases of live birth, 1 case of spontaneous abortion, and 2 cases of pregnancy maintenance. No significant differences were observed between groups A and B in the couples' age, the wives' BMI, or the numbers of ICSI oocytes and embryos transplanted (P >0.05), nor in the rates of ICSI fertilization (72.64% vs 76.17%), transferrable embryos (51.28% vs 63.24%), quality embryos (38.46% vs 49.83%), embryo implantation (17.64% vs 38.50%), abortion (0 vs 9.23%), singleton (50% vs 81.54%) and twins (50% vs 18.46%). However, the rates of clinical pregnancy (13.33% vs 42.68%, P = 0.023 <0.05) and biochemical pregnancy (13.33% vs 39.63%, P = 0.032 <0.05) were significantly lower in group A than in B.</p><p><b>CONCLUSION</b>ICSI is effective in the treatment of male infertility induced by either ADPKD or CBAVD, but the ADPKD cases have a lower success rate than the CBAVD cases in an individual cycle. The affected couples should be informed of the necessity of prenatal genetic diagnosis before embryo implantation and the inevitable vertical transmission of genetic problems to the offspring.</p>


Subject(s)
Abortion, Spontaneous , Embryo Implantation , Embryo Transfer , Female , Humans , Infertility, Male , Therapeutics , Male , Male Urogenital Diseases , Therapeutics , Oocytes , Polycystic Kidney, Autosomal Dominant , Pregnancy , Retrospective Studies , Sperm Injections, Intracytoplasmic , Vas Deferens , Congenital Abnormalities
10.
National Journal of Andrology ; (12): 1082-1086, 2013.
Article in Chinese | WPRIM | ID: wpr-267984

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the sperm DNA fragmentation index (DFI) and sperm malformation rate (SMR) before intracytoplasmic sperm injection (ICSI) and their impact on the clinical outcome of ICSI.</p><p><b>METHODS</b>This study included 79 cycles of ICSI because of oligoasthenozoospermia. We detected the sperm concentration, percentage of progressively motile sperm, DFI and SMR at 3 to 6 months before ICSI, and analyzed the relationship of DFI and SMR with the outcome parameters.</p><p><b>RESULTS</b>Of the 79 oligoasthenozoospermia cases, DFI was found to be normal (< or = 25%) in 51 and abnormal (> 25%) in the other 28, significantly increased in the latter (14.18% vs 41.47%), and coincidently, SMR, too, was normal (< or = 96%) in 51 cases and abnormal (> 96%) in 28, significantly higher in the abnormal than in the normal cases (87.88% vs 98.46%). There were no significant differences between the normal and abnormal DFI groups in age, females'BMI, number of oocytes retrieved, and number of embryos transferred, nor between the normal and abnormal SMR groups in the number of fertilized oocytes and quality embryos, biochemical pregnancy, clinical pregnancy, and early pregnancy loss. Sperm DFI was significantly positively correlated with SMR (r = 0.231, P < 0.05).</p><p><b>CONCLUSION</b>ICSI may reduce the rates of biochemical pregnancy and clinical pregnancy for men with increased sperm DFI (> 25%) and SMR (> 96%) by strict detection criteria, but with no statistically significant difference from normal males. Our findings need to be supported by further studies with larger sample sizes.</p>


Subject(s)
Adult , Chromatin , DNA Fragmentation , Female , Humans , Male , Middle Aged , Pregnancy , Pregnancy Rate , Sperm Injections, Intracytoplasmic , Spermatozoa , Pathology , Treatment Outcome , Young Adult
11.
National Journal of Andrology ; (12): 793-796, 2012.
Article in Chinese | WPRIM | ID: wpr-286423

ABSTRACT

<p><b>OBJECTIVE</b>To explore the possible mechanisms of spermatogenic arrest in severe oligoasthenoteratozoospermia induced by supernumerary, ring-neocentric 13q12.3 --> 13q22 chromosome and reciprocal deletion.</p><p><b>METHODS</b>We performed a genomic-wide high-density oaCGH analysis for a case of oligoasthenoteratozoospermia with abnormal chromosome 13 to characterize the breakpoints of the chromosome involved or the gene deletion caused by the rearrangement. We also conducted a fluorescence in situ hybridization analysis on the germ cells using probes of 13q14/13qter to observe the pairing condition of homologous chromosome 13.</p><p><b>RESULTS</b>We identified by oaCGH analysis a microdeletion of 4 consecutive probes (A_16_P19757882, A_16_P02744617, A_14_ P108858 and A_16_P02744687 at chr13q12.3: 27979261 --> 28039191) with 59.93 kb between the FLT1 and POMP genes, with no annotated genes in the deleted region. The signals of 13q14 and 13qter were separated from each other in 90% of all the primary spermatocytes examined, indicating the unpairing of homologous chromosome 13 or synapse failure.</p><p><b>CONCLUSION</b>Chromosomal rearrangement-induced spermatogenesis failure is caused by the unpairing of the homologous chromosomes involved in the first meiotic division of germ cells.</p>


Subject(s)
Adult , Asthenozoospermia , Genetics , Azoospermia , Chromosome Aberrations , Chromosome Breakpoints , Chromosomes, Human, Pair 13 , Comparative Genomic Hybridization , Humans , Male , Meiosis , Oligospermia , Genetics , Spermatogenesis , Genetics
12.
Article in Chinese | WPRIM | ID: wpr-839787

ABSTRACT

Objective To investigate ZEB-l protein expression in hepatocellular carcinoma (HCC) tissues and the relevant significance. Methods Immunohistochemical method and Western blotting analysis were used to detect the ZEB-l protein expression in 62 HCC tissues,48 paracancerous cirrhotic tissues and 10 normal liver tissues. The relationship between ZEB-l protein expression and clinicopathological features of HCC was evaluated. Results Immunohistochemical staining showed that the positive rates of ZEB-l protein expression in HCC tissues and paracancerous cirrhotic tissues were 93. 5% (58/ 62) and 83.3 (40/48), respectively. Western blotting analysis showed that ZEB-l protein expression in HCC tissues was significantly higher than those in the paracancerous cirrhotic tissues and normal liver tissues (P0. 05). Conclusion High expression of ZEB-l is correlated with the development and progression of HCC. ZEB-l might also participate in the epithelial-mesenchymal transition of HCC cells, subsequently contributing to the invasion and metastasis.

13.
National Journal of Andrology ; (12): 771-774, 2011.
Article in Chinese | WPRIM | ID: wpr-305793

ABSTRACT

<p><b>OBJECTIVE</b>To compare the outcomes of intracytoplasmic sperm injection (ICSI) for infertile males with Y-chromosome microdeletions and for those with azoospermia or severe oligospermia but without Y-chromosome microdeletions.</p><p><b>METHODS</b>We retrospectively analyzed 56 cycles of ICSI for 48 infertile cases with Y microdeletions (Group A) and 94 cycles for 90 cases with azoospermia or severe oligospermia but without Y-chromosome microdeletions (Group B) during the same period. We compared the two groups in the females' age, duration of infertility, males' age, number of oocytes retrieved, number of ICSI oocytes, fertilization rate, good embryo rate, number of embryos transferred, implantation rate, clinical pregnancy rate, abortion rate, live birth rate and babies' sexes.</p><p><b>RESULTS</b>There were no significant differences between Groups A and B in the females' age, duration of infertility, males' age, number of oocytes retrieved, number of ICSI oocytes and number of embryos transferred (P > 0.05), nor in the rates of fertilization (69.0% vs 73.2%), good embryos (53.3% vs 48.7%), implantation (24.0% vs 30.3%), biochemical pregnancy (41.1% vs 44.7%), clinical pregnancy (37.5% vs 35.1%), early abortion (4.8% vs 6.1%) and live birth (35.7% vs 29.2%) (P > 0.05).</p><p><b>CONCLUSION</b>Y-chromosome microdeletions do not affect the outcomes of ICSI. The affected couples should be informed of the necessity of prenatal genetic diagnosis before embryo implantation and the inevitability of vertical transmission to male offspring.</p>


Subject(s)
Adult , Azoospermia , Genetics , Therapeutics , Chromosome Deletion , Chromosomes, Human, Y , Genetics , Female , Humans , Infertility, Male , Genetics , Therapeutics , Male , Oligospermia , Genetics , Therapeutics , Pregnancy , Pregnancy Rate , Retrospective Studies , Sex Chromosome Aberrations , Sex Chromosome Disorders of Sex Development , Genetics , Therapeutics , Sperm Injections, Intracytoplasmic , Treatment Outcome
14.
National Journal of Andrology ; (12): 237-241, 2011.
Article in Chinese | WPRIM | ID: wpr-266184

ABSTRACT

<p><b>OBJECTIVE</b>To investigate and compare the incidences of birth defects in the offspring conceived by assisted reproductive technology (ART), including artificial insemination with the donor's semen (AID), in vitro fertilization with donor's semen (IVF-D) and intracytoplasmic sperm injection with the donor's semen (ICSI-D), and in those conceived through ART with the husband's semen, including AIH, IVF and ICSI, in order to further evaluate the safety of ART with the donor's semen.</p><p><b>METHODS</b>From January 2005 to October 2009, Shanghai Human Sperm Bank provided sperm copies to 11 medical institutions, which resulted in a total of 904 offspring born by ART. We followed up all these cases and investigated the status of the offspring. The control group included 4195 offspring of infertile couples from 4 Reproductive Medical Centers approved by health management administrations, which were conceived by ART in the same period. After investigating the number of offspring and cases of birth defects caused by various methods of assisted reproductive technology, we compared the incidence of birth defects resulting from the donor's semen and that from the husband's.</p><p><b>RESULTS</b>There were 7 cases of birth defects (0.77%) in the offspring born by ART with the donor's semen, and 42 cases (1.00%) in those born by ART with the husband's semen, with no significant differences between the two groups (P > 0.05).</p><p><b>CONCLUSION</b>There were no significant differences in the category of birth defects between ART with the donor's semen and that with the husband's, while the incidence of birth defects resulting from ART with the donor's semen was significantly lower than that from ICSI in infertile couples. The present findings indicate a higher safety of ART with the donor's semen.</p>


Subject(s)
Congenital Abnormalities , Epidemiology , Fertilization in Vitro , Methods , Humans , Infertility , Male , Semen , Sperm Banks , Sperm Injections, Intracytoplasmic , Methods , Tissue Donors
15.
Article in Chinese | WPRIM | ID: wpr-235125

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of surface modification of titanium on OPG/RANKL mRNA expression in human osteoblast-like cells.</p><p><b>METHODS</b>MG-63 osteoblast-like cells were seeded on the titanium plates with surface polishing and with surface modification by sandblasting plus acid-base treatment, with the cells on glass slides as the control. On days 2, 4, 6, and 8 following cell seeding, the cells were harvested for examination of OPG/RANKL mRNA expression using RT-PCR and real-time PCR.</p><p><b>RESULTS</b>The expression of OPG/RANKL mRNA was sensitive to the surface microphotography. Compared with the other groups, the cells on the titanium plates with sandblasting plus acid-base treatment, which resulted in a porous micro-structure and high roughness, showed significantly up-regulated expression of OPG mRNA. OPG mRNA expression also showed a time-dependent up-regulation, and was the highest on day 8. The expression of the RANKL mRNA in cells on both of the titanium plates was higher than that in the control cells. The peak level of RANKL mRNA expression occurred on day 6 followed by a gradual decrease.</p><p><b>CONCLUSION</b>A rough and porous surface of the culture plates and prolonged culture time can synergistically up-regulate the ratio of OPG/RANKL mRNA.</p>


Subject(s)
Cell Culture Techniques , Cell Line , Humans , Osteoblasts , Cell Biology , Metabolism , Osteoprotegerin , Genetics , Metabolism , Porosity , RANK Ligand , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism , Surface Properties , Tissue Scaffolds , Titanium , Chemistry , Pharmacology
16.
Article in Chinese | WPRIM | ID: wpr-235089

ABSTRACT

<p><b>OBJECTIVE</b>The objective of this study is to investigate the influence of zirconia content which is 0-30.0% weight percentage of matrix on translucency of zirconia-toughened alumina glass-infiltrated ceramics.</p><p><b>METHODS</b>Seven groups were divided according to different weight percentage of zirconia (0, 2.5%, 5.0%, 7.5%, 10.0%, 20.0% and 30.0%). After sintering, infiltrating and polishing, spectral transmittance was determined with spectrophotometer under D65 standard source. Contrast ratio was also tested by whiteness colorimeter.</p><p><b>RESULTS</b>With mass fraction of zirconia increasing from 0 to 30.0%, spectral transmittance reduced from 0.406% to 0.058%, while contrast ratio value increased from 0.849 +/- 0.005 to 1.015 +/- 0.006. When zirconia content was 10.0%, contrast ratio was 0.990 +/- 0.008. When it was more than 10.0%, transmission rate of the downward trend and contrast ratio of the rising trend became flat.</p><p><b>CONCLUSION</b>Zirconia content has a direct impact on translucency of zirconia-toughened alumina glass-infiltrated ceramic, which is essentially opaque when zirconia content is 10.0%. When mass fraction of zirconia is more than 10.0%, the influence of zirconia content is reduced.</p>


Subject(s)
Aluminum Oxide , Ceramics , Dental Materials , Dental Porcelain , Glass , Zirconium
17.
National Journal of Andrology ; (12): 977-983, 2011.
Article in Chinese | WPRIM | ID: wpr-239048

ABSTRACT

<p><b>OBJECTIVE</b>Sperm chromatin structure assay (SCSA), as a clinically practical technique for the analysis of DNA damage, is rarely reported in China. This study focuses on the correlation of DNA damage with the pregnancy rate of intrauterine insemination (IUI).</p><p><b>METHODS</b>We performed semen analysis for 482 couples undergoing IUI, calculated the DNA fragmentation index (DFI) by SCSA, and observed the relationship between DFI and the pregnancy rate of IUI.</p><p><b>RESULTS</b>Clinical pregnancy was achieved in 5 (5.26%) of the 95 cases with DFI > 25%, and in 59 (15.25%) of the 387 cases with DFI < or = 25%. Those with sperm DFI >25% had significantly lower rates of biochemical pregnancy and clinical pregnancy than those with DFI < or = 25% (OR: 0.37, 95% CI: 0.14 - 0.96 and OR: 0.38, 95% CI: 0.16 - 0.97). No significant differences were found in the DFI of 54 cases between the first and the second cycle ([15.05 +/- 7.98]% vs [17.25 +/- 12.18]%, P > 0.05). Sperm DFI was significantly negatively correlated with sperm concentration, sperm motility and total progressively motile sperm count (P < 0.01).</p><p><b>CONCLUSION</b>The pregnancy rate of IUI is significantly lower in couples with DFI >25% than in those with DFI < or = 25%. Sperm DFI obtained from SCSA is partly correlated with sperm concentration and motility, and it is a robust predictor of the IUI outcome.</p>


Subject(s)
Adult , Chromatin , Chromosome Structures , DNA Fragmentation , Female , Humans , Insemination , Insemination, Artificial , Methods , Male , Pregnancy , Pregnancy Outcome , Genetics , Pregnancy Rate , Reproductive Techniques, Assisted , Sperm Count , Sperm Motility , Young Adult
18.
National Journal of Andrology ; (12): 345-348, 2010.
Article in Chinese | WPRIM | ID: wpr-295061

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the phenotype, pathogenesis and molecular biological features of 46, XX testicular disorder of sex development.</p><p><b>METHODS</b>We obtained the history of 2 patients with 46, XX testicular disorder of sex development, examined the cavitas pelvis by type-B ultrasonography, analyzed the karyotype of the chromosome, and detected the genes SRY, YRRM1, DYS240 and DAZ by PCR amplification.</p><p><b>RESULTS</b>Microrchidia, azoospermia and maldevelopment of secondary sex characteristics were observed in both of the patients, but ultrasonography revealed no female internal genitals. Their chromosome gender was karyotyped as 46, XX, with the SRY gene positive in both, but the YRRM1 gene positive in only one of the cases.</p><p><b>CONCLUSION</b>Chromosome karyotyping and detection of the SRY gene for patients with abnormal sex development can give us an insight into the genetic pathogenesis and provide us with scientific evidence for the diagnosis and treatment of the condition.</p>


Subject(s)
Adult , Genes, sry , Gonadal Dysgenesis, 46,XX , Genetics , Humans , Male , Nuclear Proteins , Genetics , RNA-Binding Proteins , Genetics
19.
Article in Chinese | WPRIM | ID: wpr-350261

ABSTRACT

<p><b>OBJECTIVE</b>To investigate morphological change of osteoblasts cultured on titanium plates with different microarchitecture structure when exposured to fluid shear stress.</p><p><b>METHODS</b>14 dynes x cm(-2) fluid shear stress was applied on osteoblasts cultured on 3 different commercially pure titanium plates: Polished treatment (PT), sandblast (SB), sandblasting and acid-base (SB-AB) surfaces. Scanning electron microscope (SEM) was adopted to observe the morphological changes after 0.5, 4, 7.5 h time point respectively.</p><p><b>RESULTS</b>Morphologically, no significant changes were observed after 0.5 h and few osteoblasts were seen after 7.5 h on all 3 type of different surfaces, and significant changes could only be observed after 4 h. Osteoblasts were elongated and rearranged along the flow way on different levels on PT surface. Shape of cells was altered, from long fusiform suspending over depressed areas into polygon stretching out many synapsises tightly attached to pits on SB-AB surface. Osteoblasts on SB surface displayed similar change as SB-AB surface, besides, some cells were elongated along the way of flow, stretching out threadlike synapsises attached to edges of pits.</p><p><b>CONCLUSION</b>Morphological change of osteoblast responding to fluid shear stress in physiological range depends on substrate microarchitecture and varies with the time of fluid shear stress application.</p>


Subject(s)
Microscopy, Electron, Scanning , Osteoblasts , Stress, Mechanical , Titanium
20.
Article in Chinese | WPRIM | ID: wpr-360998

ABSTRACT

<p><b>OBJECTIVE</b>Posterolateral intertransverse process fusion was performed in aged and young adult female rabbits lumbar spine using recombinant human bone morphogenetic protein-2 (rhBMP-2) and autograft to reveal the function of rhBMP-2 on spinal fusion on aged animals.</p><p><b>METHODS</b>A total of 24 female New Zealand white rabbits included 12 young adult of 6 months and 12 aged of 2-year-old, was divided into 4 groups: (1) young adult autologous iliac crest bone group [ICBG(Y), n=6); (2) young adult rhBMP-2/absorbable collagen sponge (ACS) group [BMP-2(Y), n=6]; (3) aged autologous iliac crest bone group [ICBG(O), n=6]; aged rhBMP-2/ACS group [BMP-2(O), n=6]. All were underwent posterolateral fusion in same day. rhBMP-2 and autologous iliac crest bone was implant bilateral LS-L6 intertransverse processes, respectively. Half of the rabbits were sacrificed at 3.6 weeks following surgery, respectively. The results were assessed by manual palpation, radiographs, computed tomographic scans (3D) and histology.</p><p><b>RESULTS</b>Six weeks after surgery, radiography, computed tomography and histology indicated the different result in healing in the posterolateral fusion using rhBMP-2 compared to ICBG (P < 0.05). Aged BMP-2 group showed significantly higher fusion rates than Aged ICBG group.</p><p><b>CONCLUSION</b>This study demonstrated rhBMP-2 can increase the posterolateral fusion rate and new bone quality in aged rabbitss than autograft, it may take the place of ICBG. But its role is effected by age.</p>


Subject(s)
Aging , Animals , Bone Morphogenetic Protein 2 , Pharmacology , Female , Humans , Palpation , Rabbits , Recombinant Proteins , Pharmacology , Spinal Cord , Pathology , General Surgery , Transplantation , Spinal Fusion , Tomography, X-Ray Computed , Transplantation, Autologous
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