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1.
Chinese Journal of Biotechnology ; (12): 4861-4873, 2023.
Article in Chinese | WPRIM | ID: wpr-1008064

ABSTRACT

The aim of this study was to produce Erns protein of bovine viral diarrhea virus (BVDV) by using suspensively cultured CHO cells expression system and to analyze the immunogenicity of the purified Erns protein. In this study, the recombinant eukaryotic expression plasmid pcDNA3.1-BVDV-Erns was constructed based on the gene sequence of BVDV-1 NADL strain. The Erns protein was secreted and expressed in cells supernatant after transfecting the recombinant expression plasmid pcDNA3.1-BVDV-Erns into CHO cells. The expression and purification of the Erns protein was analyzed by SDS-PAGE, the reactivity was determined with anti-His monoclonal antibodies and BVDV positive serum with Western blotting. Immunogenicity analysis of the Erns protein was determined after immunizing New Zealand white rabbits, and the serum antibodies were tested by indirect ELISA (iELISA) and indirect immunofluorescence (IFA). The serum neutralizing titer of the immunized rabbits was determined by virus neutralization test. The concentration of the purified Erns protein was up to 0.886 mg/mL by BCA protein quantification kit. The results showed that the Erns protein could be detected with anti-His monoclonal antibodies and anti-BVDV sera. Serum antibodies could be detected by iELISA on the 7th day post-prime immunization, and the antibody level was maintained at a high titer until the 28th day post-immunization. The antibody titer was 1:128 000. Furthermore, the expression of the Erns protein in BVDV-infected MDBK cells could be detected with immunized rabbits sera by IFA. Moreover, antigen-specific neutralizing antibodies of 2.71 log10 was induced in rabbits. In this study, purified BVDV Erns protein was successfully produced using CHO suspension culture system, and the recombinant protein was proved to have a good immunogenicity, which may facilitate the development of BVD diagnosis method and novel subunit vaccine.


Subject(s)
Rabbits , Animals , Cricetinae , Cricetulus , CHO Cells , Antibodies, Viral , Diarrhea Viruses, Bovine Viral/genetics , Antibodies, Monoclonal/genetics , Diarrhea , Viral Vaccines/genetics
2.
Chinese Journal of Orthopaedics ; (12): 951-958, 2023.
Article in Chinese | WPRIM | ID: wpr-993526

ABSTRACT

Objective:To compare the clinical efficacy of anatomical reconstruction of coracoclavicular ligament at the original insertion point and clavicular hook plate fixation in the treatment of acromioclavicular joint dislocation.Methods:Retrospective analysis was made on the data of 67 patients with acromioclavicular joint dislocation who received surgical treatment in the Department of Orthopaedics of the First Affiliated Hospital of Nanjing Medical University from June 2015 to January 2021. According to the surgical method, they were divided into reconstruction group (using the technique of anatomical reconstruction of coracoclavicular ligament at the original insertion point) and hook plate group (using the clavicular hook plate). There were 37 cases in the reconstruction group, including 26 males and 11 females, aged 47.2±9.6 years (range, 18-65 years), 13 cases on the left and 24 cases on the right. Among the 37 patients, 8 were sports injuries, 14 were falls, 11 were traffic accidents, and 4 were external force injuries. The average time from injury to surgery was 8.3±2.3 days. There were 30 cases in the hook plate group, including 24 males and 6 females, aged 47.4±9.7 years (range, 18-67 years), 12 cases on the left and 18 cases on the right. Among the 30 patients, 7 were sports injuries, 11 were falls, 9 were traffic accidents, and 3 were external force injuries. The average time from injury to surgery was 7.9±2.6 days. The surgical time, incision length, intraoperative bleeding, hospital stay, postoperative coracoclavicular separation ratio, and postoperative complications were compared between the two groups. Constant-Murley score and visual analog scale (VAS) were used to assess the shoulder joint function and pain degree of patients.Results:Both groups of patients were followed up, with a follow-up time of 12.3±0.4 months for the reconstruction group and 12.2±0.5 months for the hook plate group. The operation time (105.8±10.0 min), incision length [12.0 (11.0, 13.0) cm] and hospitalization time (6.8±2.1 d) in the reconstruction group were longer than those in the hook plate group [48.3±4.9 min, 10.0 (10.0, 11.0) cm, and 5.5±2.7 d], while the intraoperative blood loss (75.1±3.9 ml) was less than that in the hook plate group (90.3±6.3 ml), the differences were statistically significant ( P<0.05). The VAS [4.0 (3.0, 5.0), 3.0 (3.0, 3.0), 2.0 (1.0, 2.0) points] and Constant-Murley score (65.4±4.5, 84.9±2.5, 90.1±2.5 points) of the reconstruction group at 3 days, 3 months, and 12 months after surgery were better than those of the hook plate group [5.0 (4.0, 5.0), 4.0 (4.0, 4.0), 3.0 (3.0, 4.0) and 56.9±3.5, 79.6±4.0, 86.8±2.4 points], the difference was statistically significant ( P<0.05). At the last follow-up, there was a statistically significant difference in the separation ratio of coracoclavicular distance between the reconstruction group (0.12±0.08) and the hook plate group 0.22±0.15 ( t=3.25, P=0.002). There was no significant difference ( Z=-0.52, P=0.605) in the separation ratio of acromioclavicular distance [0.16 (0.05, 0.25) and 0.16 (0.04, 0.40)]. In the hook plate group, 6 cases had shoulder joint foreign body sensation and 2 cases had acromioclavicular joint redislocation (both Rockwood type III). Because the shoulder joint function did not affect their daily life, neither patient underwent secondary surgery. And no case of acromioclavicular joint redislocation occurred in the reconstruction group. Conclusion:Compared with the clavicular hook plate fixation, anatomic reconstruction of coracoclavicular ligament at the original insertion point in the treatment of acromioclavicular joint dislocation can reduce the pain of the shoulder joint earlier, which has the characteristics of small trauma, good effect, and reduces the steps of internal fixation removal, and has good clinical curative effect.

3.
Chinese Journal of Trauma ; (12): 145-152, 2023.
Article in Chinese | WPRIM | ID: wpr-992582

ABSTRACT

Objective:To compare the effect of reduction and internal fixation of composite acetabular fracture with the modified two-window iliofemoral approach and ilioinguinal approach.Methods:A retrospective cohort study was used to analyze the clinical data of 160 patients with composite acetabular fracture admitted to First Affiliated Hospital of Nanjing Medical University from January 2016 to August 2021, including 117 males and 43 females, aged 15-78 years [(44.1±16.0)years]. According to the Letournel classification system, there were 101 patients with both-column fracture, 5 with anterior wall/column combined with posterior semi-transverse fracture and 24 with T-shaped fracture. A total of 80 patients were treated using the modified iliofemoral incision combined with limited Pfannstiel incision (modified two-window iliofemoral approach group) and the other 80 patients were treated using the ilioinguinal approach (ilioinguinal approach group). The fracture healing was observed. The operation time and intraoperative bleeding volume were compared between the two groups. The quality of fracture reduction was evaluated by Matta scoring standard at 1 day and 6 months after operation. The modified Merle d′Aubigne & Postel scoring standard was used to evaluate the function of the affected hip joint at the last follow-up. The incidence of complications such as neurovascular injury, iatrogenic bladder injury, heterotopic ossification and femoral head necrosis were compared between the two groups.Results:All patients were followed up for 12-78 months [(43.3±17.9)months], with bony union of the fracture. The operation time and intraoperative bleeding volume in modified two-window iliofemoral approach group were 150.0 (123.8, 180.0)minutes and 600.0 (500.0, 787.5)ml when compared to 190.0 (150.0, 240.0)minutes and 700.0 (562.5, 887.5)ml in ilioinguinal approach group (all P<0.01). There was no significant difference between the two groups in the quality of fracture reduction at 1 day and 6 months after operation, function of hip joint at the last follow-up and incidence of complications (all P>0.05). Conclusions:For reduction and internal fixation of composite acetabular fracture, the modified two-window iliofemoral approach has advantages over the ilioinguinal approach in reducing operation time and intraoperative bleeding, although both methods yield similar results in fracture reduction quality, postoperative hip function and complication rate.

4.
Chinese Journal of Orthopaedics ; (12): 403-412, 2022.
Article in Chinese | WPRIM | ID: wpr-932849

ABSTRACT

Objective:To investigate the radiographic risk factors related to the occurrence of distal adding-on (AO) in posteriorly treated Lenke modifier C adolescent idiopathic scoliosis (AIS) patients with the apical vertebra of the lumbar curve (L-AV) selected as the lowest instrumented vertebra (LIV).Methods:Seventy-three Lenke modifier C AIS patients were analyzed with a minimum of 2-year follow-up after posterior spinal fusion surgery with L-AV selected as LIV. Patients were grouped according to the occurrence of distal AO. Radiographical parameters were measured as follows: Cobb angle, curve flexibility and AV translation of the thoracic curve and lumbar curve, L-AV rotation and tilt, coronal balance, Harrington stable zone on anteroposterior (AP) film and concave bending film, L-AV derotation and L-AV/AV+1 disc opening or closing on convex bending film, etc. The Scoliosis Research Society-22 (SRS-22) score was used to evaluate clinical outcomes. Radiographic and clinical parameters were statistically analyzed between the two groups.Results:There were 23 patients in AO group and 50 patients in non-AO group. Preoperatively, the AO group had proximal L-AV, lower flexibility of the thoracic curve, coronal imbalance shifted to the convex side of the lumbar curve, lower Harrington stable zone on AP film and concave bending film, and less L-AV/AV+1 disc opening on convex bending film compared to non-AO group. The logistic regression revealed that the flexibility of the thoracic curve, coronal balance, Harrington stable zone on concave bending film, and L-AV/AV+1 disc opening or closing on convex bending film were significant predictors of distal AO. Specifically, the flexibility of the thoracic curve >40.0%, coronal balance< 19.6mm, and Harrington stable zone on concave bending film >77.8% might be optimal thresholds for selecting L-AV as LIV. At the final follow-up, AO group had larger lumbar curves and lower correction rates. No difference was found in the SRS-22 between the two groups.Conclusion:For Lenke modifier C AIS patients, LIV might be considered to stop at L-AV if there were good flexibility of the thoracic curves, coronal balance, L-AV/AV+1 disc opening on convex bending film, and large Harrington stable zone on concave bending film.

5.
Chinese Journal of Orthopaedic Trauma ; (12): 421-428, 2022.
Article in Chinese | WPRIM | ID: wpr-932349

ABSTRACT

Objective:To compare the clinical efficacy between coracoclavicular ligament anatomical reconstruction and clavicular hook plate in the treatment of Neer Ⅱb distal clavicular fractures.Methods:A total of 64 patients with Neer Ⅱb clavicular fracture were treated at Department of Orthopaedics, The First Affiliated Hospital to Nanjing Medical University from September 2016 to June 2019. They were 35 males and 29 females, aged from 19 to 68 years (average, 50.7 years). They were assigned into 2 groups according to their operative methods: a reconstruction group of 30 cases undergoing coracoclavicular ligament anatomical reconstruction and a hook plate group of 34 cases undergoing fixation with a clavicular hook plate. The 2 groups were compared in terms of hospital stay, operation time, intraoperative blood loss, surgical incision length, postoperative coracoclavicular separation ratio, visual analogue scale (VAS) and Constant-Murley shoulder scores at 3, 6 and 12 months after operation, and postoperative complications.Results:There was no significant difference in general data between the 2 groups, showing comparability between groups ( P>0.05). Operations were completed uneventfully and surgical incisions healed by primary intention in both groups after operation. All the patients were followed up for 12 to 24 months (average, 14.6 months). The operation time [(74.6±22.0) min] and incision length [(10.4±0.4) cm] were significantly shorter but the intraoperative blood loss [(90.2±5.3) mL] was significantly less in the hook plate group than those in the reconstruction group [(95.6±20.8) min, (12.4±0.9) cm and (74.2±3.5) mL] ( P<0.05). There was no significant difference in hospital stay between the 2 groups ( P>0.05). At 3, 6 and 12 months after operation, the VAS scores (1.8±0.5, 1.2±0.3 and 1.1±0.2) and Constant-Murley scores (85.2±4.6, 91.1±2.6 and 92.1±2.2) in the reconstruction group were significantly better than those in the hook plate group (3.2±1.0, 1.6±0.3 and 1.5±0.3; 73.6±2.9, 85.9±4.6 and 87.0±3.1) ( P<0.05). At the last follow-up, the postoperative coracoclavicular separation ratio (elevation) in the hook plate group (0.20±0.16) was significantly greater than that in the reconstruction group (0.10±0.05) ( P<0.05). Conclusion:In the treatment of Neer ⅡB distal clavicular fractures, coracoclavicular ligament anatomical reconstruction may lead to better fixation and fewer postoperative complications than a clavicular hook plate, demonstrating fine clinical efficacy.

6.
Chinese Journal of Orthopaedic Trauma ; (12): 1088-1093, 2022.
Article in Chinese | WPRIM | ID: wpr-992672

ABSTRACT

Objective:To investigate the injury characteristics, treatment strategy and prognosis of acetabular fracture combined with ipsilateral femoral neck fracture.Methods:A retrospective analysis was conducted of the 15 patients with acetabular fracture combined with ipsilateral femoral neck fracture who had been treated from January 2009 to June 2021 at Department of Orthopaedics, The First Hospital Affiliated to Nanjing Medical University. They were 10 males and 5 females, aged from 26 to 68 years (average, 47.1 years). The treatment strategy depended on their injury characteristics. For the 3 patients with incomplete displacement of the femoral neck fracture but no hip dislocation, the femoral neck fracture was treated by closed reduction and internal fixation; for the 7 patients with complete displacement of the femoral neck fracture but no hip dislocation, the femoral neck fracture was treated first by closed reduction and then by open reduction and internal fixation in case the closed reduction had failed; for the 5 patients complicated with hip dislocation (anterior one in 2 cases and posterior one in 3 cases), the femoral neck fracture was treated by open reduction and internal fixation. The acetabular fractures were treated via an appropriate surgical approach depending on their classifications. The operation time, intraoperative bleeding, fracture healing, functional recovery and complications such as postoperative avascular necrosis of the femoral head (NFH) were recorded.Results:In this cohort, the operation time ranged from 170 to 540 min, averaging 210 min, and the amount of intraoperative bleeding from 300 to 7,900 mL, averaging 800 mL. Postoperative X-ray films showed that all acetabular fractures and femoral neck fractures achieved anatomical reduction or satisfactory reduction. All patients were followed up for 1 to 13 years (average, 4 years). One patient had to receive total hip arthroplasty due to nonunion one year after operation, and the fractures in the other 14 patients healed by the first intention. At the last follow-up by the Merle d'Aubigné & Postel scoring, the function of the hip affected was excellent in 3 cases, good in 6 and poor in 6. NFH occurred in 4 cases, of which one had no hip dislocation and 3 had hip dislocation. Ectopic ossification developed in one patient after operation.Conclusions:The incidence of NFH is high in the patients with acetabular fracture combined with ipsilateral femoral neck fracture, especially higher in those complicated with hip dislocation. Treatment strategies should vary according to the injury characteristics. Attention should be paid to protection of the blood supply to the femoral head which significantly improves the prognosis.

7.
Chinese Journal of Orthopaedics ; (12): 1708-1716, 2021.
Article in Chinese | WPRIM | ID: wpr-910764

ABSTRACT

Abstract:To compare the morphology of spinal cord between healthy adolescents with adolescent Chiari malformation type I (CMI) patients and investigate the impact of syringomyelia on the morphology of spinal cord in CMI patients.Methods:The clinical and radiological data of 292 CMI patients diagnosed by our center between June 2012 and March 2019 were retrospectively reviewed. Among them 15 CMI patients without syringomyelia were recruited in the CM group. Among the remaining 277 CMI patients, 274 patients had syringomyelia below the C 3-4 intervertebral disc. According to the principle of best matching, CMI patients with syringomyelia were selected with the closest age to the CM group (±18 months), and 30 CMI patients with syringomyelia were included in the CMS group according to a ratio of 1∶2. Thirty healthy adolescents were enrolled as the control group (NC group) in the same way. The anteroposterior diameters of spinal cord at C 2 (DSCO-C 2), spinal canal at C 2 (DSCA-C 2), midbrain-pontine junction (DPJ), the distance between the tip of cerebellar tonsils and the foramen magnum (AB) and the maximal diameter of the syrinx (D-syrinx) were measured on MRI. All radiographic parameters were measured twice independently by two spine surgeons, and intraclass correlation coefficient (ICC) were determined to demonstrate intra- and inter-observer reliability. One-way ANOVA and SNK- q test were used to compare the above radiographic parameters and age between CM, CMS and NC group. The distribution of genders was compared between the three groups using Chi-square tests. Pearson correlation analysis were conducted to demonstrate the relationship between radiographic parameters in CM and CMS group. Results:ICC ranged between 0.91 and 0.95 in the current study, demonstrating "excellent" reliability of radiographic measurements. No significant difference was noted regarding age and the distribution of genders among the three groups. Patients in CM and CMS groups showed similar DSCO-C 2 values ( P=0.254), both of which were significantly lower than that in NC group ( P<0.001). DSCA-C 2 in CMS group was significantly larger than that in CM ( P=0.003) and NC ( P<0.001) groups, while no significant difference was found between the CM and NC groups ( P=0.216). Moreover, DPJ in CMS group was significantly lower than that in CM group ( P<0.001) and NC group ( P<0.001). There was no significant difference in AB between CM and CMS groups ( P=0.948). DSCO-C 2 was significantly positively correlated with DSCA-C 2 in CMS group ( r=0.906, P<0.001), while AB, D-syrinx, DSCO-syrinx, DSCA-syrinx and DPJ were not significantly correlated with DSCA-C 2. There were significant correlations observed between DPJ and other radiographic parameters in the CMS group (all P>0.05). Significant positive correlation between DSCO-C 2 and DPJ was observed in CM group ( r=0.703, P=0.005). There was no significant correlation between DSCO-C 2 and DSCA-C 2 and DPJ in NC group (all P>0.05). Conclusion:CMI adolescents have significant atrophic change of cervical spinal cord and midbrain-pontine junction compared with healthy adolescents, regardless of the existence of syrinx. Moreover, syrinx in CMI patients indicated more obvious atrophic change of midbrain-pontine junction and dilated spinal canal compared with isolated CMI patients.

8.
Chinese Journal of Orthopaedics ; (12): 881-891, 2021.
Article in Chinese | WPRIM | ID: wpr-910670

ABSTRACT

Objective:The purpose of the study is to investigate how to select lowest instrumented vertebra (LIV) in posterior spinal corrective surgery for Lenke 1A/2A adolescent idiopathic scoliosis (AIS) patients,and to further identify the risk factor for postoperative distal adding-on.Methods:FromJanuary 2008 to January 2014, a total of 85 Lenke 1A/2A AIS patients with one level proximal to last substantially touching vertebra (LSTV-1) selected as LIV were enrolled in the study. There were 45 Lenke 1A and 40 Lenke 2A, and 70 females and 15 males. The average age of surgery was 14.4±2.2 years (10-18 years). They were followed up over 2 years. The upright posteroanterior and lateral radiographs were performed preoperatively, immediately after surgery and at the final follow-up. Several radiographic parameters were measured such as Cobb angle, thoracic curve length, apex location, LIV rotation, deviated distance of LIV from central sacral vertical line, coronal balance and sagittal balance, etc. Distal adding-on was defined as a progressive increase in the number of vertebrae in the distal curve at the last follow-up. Patients were classified into adding-on and non adding-on group. The risk factors associated with the incidence of adding-on were analyzed. Subgroup analysis were further performed according to the curve type.Results:The mean duration of follow-up was 37.8±16.3 months (24-95 months). The average Cobb angle of main thoracic curve was 51.9°±6.8° (42°-85°). At the last follow-up, 36 patients (42.4%) had ideal outcome without distal adding-on. For Lenke 1A patients, the risk factor for adding-on included: low Risser ( t=2.730, P=0.005), long thoracic curve ( t=1.930, P=0.030) with low apex ( t=1.734, P=0.045), preoperative large rotation and deviation of the LSTV-1 ( t=2.319, P=0.013; t=3.288, P=0.001), and preoperative coronal imbalance ( t=1.729, P=0.046). For Lenke 2A patients, the risk factor for adding-on included: low Risser ( t=2.246, P=0.015), preoperative large rotation and deviation of the LSTV-1 ( t=2.534, P=0.008; t=1.972, P=0.028), and preoperative coronal imbalance ( t=1.702, P=0.048). Conclusion:When choosing LSTV-1 as LIV, skeletal immaturity, large rotation and deviation of LSTV-1 and preoperative coronal imbalance are risk factors for distal adding-on in Lenke 1A/2A curves; Also, long thoracic curve with low apex is associated with distal adding-on in Lenke 1A curves. Therefore, for skeletal immature patients with large rotation and deviation of LSTV-1, preoperative coronal imbalance and long thoracic curve with low apex,the 'LSTV’ rule should be followed to decrease the incidence of distal adding-on. While in other case, it could safely distally stop at LSTV-1.

9.
Chinese Journal of Trauma ; (12): 1099-1104, 2021.
Article in Chinese | WPRIM | ID: wpr-909982

ABSTRACT

Objective:To investigate the clinical efficacy of posteromedial approach through anterior border of tibialis posterior tendon combined with posterolateral approach in open reduction and internal fixation of Klammer type II/III posterior pilon variant fracture.Methods:A retrospective case series study was conducted on 37 patients with posterior pilon variant fracture treated by posteromedial approach through anterior border of tibialis posterior tendon combined with posterolateral approach in Jiangsu Province People's Hospital and its Liyang branch between January 2015 and December 2019. There were 17 males and 20 females, aged 25-65 years[(49.5±9.3)years]. Klammer classification was type II in 20 patients and type III in 17 patients. The quality of fracture reduction, incision healing and postoperative complications were observed. The loss of fracture reduction or implant failure was detected during the follow-up. The fracture union time was recorded. At the final follow-up, the residual pain was assessed by the visual analogue scale(VAS)and the ankle function by the American Orthopedic Foot and Ankle Society(AOFAS)ankle-hindfoot score.Results:All patients were followed up for 12-20 months[(15.6±1.2)months]. An anatomic fracture reduction was observed in all patients, showing the incision healing by first intention. No skin necrosis, posteromedial neurovascular bundle injury, deep venous thrombosis, checkrein deformity or other complications occurred. During the follow-up, there was no reduction loss or implant failure. The fracture healing time ranged from 11 to 16 weeks[(13.2±1.4)weeks]. At the final follow-up, the VAS was decreased from 6-9 points[(8.0±0.8)points]preoperatively to 0-2 points[(1.2±0.6)points]( P<0.01). At the final follow-up, the AOFAS ankle-hindfoot score ranged from 80 to 100 points[(89.7±4.6)points], which showed excellent results in 15 patients and good in 22 patients, with the excellence rate of 100%. Conclusion:The posteromedial approach through anterior border of tibialis posterior tendon combined with posterolateral approach in open reduction and internal fixation of Klammer type II/III posterior pilon variant fracture can achieve good fracture reduction, with low incidence of complications, pain relief and satisfactory ankle function recovery.

10.
Chinese Journal of Orthopaedics ; (12): 199-207, 2020.
Article in Chinese | WPRIM | ID: wpr-868969

ABSTRACT

Objective:To investigate the radiological presentations in relation to different ages in scoliosis associated with Chiari malformation typeⅠ(CMI).Methods:A retrospective analysis was performed on 80 patients diagnosed with scoliosis associated with CMI from June 2010 to June 2018, who were classified into three groups according to their age: Children(age≤10 years), Adolescents (age 11-18 years) and Adults (age≥19 years). Curves were classified into typical and atypical patterns in the coronal plane. The coronal and sagittal radiographical parameters were measured in the three groups. Moreover, cerebellar tonsillar descent and syringomyelia patterns were measured on MRI, and the parameters among the three groups were compared statistically.Results:The incidence of atypical curve patterns in Children (10 patients), Adolescents (44 patients) and Adults (26 patients) was 30.0%, 15.9%, and 50.0%, respectively (χ 2=2.654, P=0.265). There was no statistical difference in the distribution of curve patterns among CMI patients with different age. In the coronal profile, Cobb angle ( F=16.751, P<0.001) and flexibility ( F=3.285, P=0.044) of main curve, Cobb angle of secondary curve ( F=9.805, P<0.001) and coronal balance(CB) ( F=5.249, P=0.007) showed statistical difference. The elderly patients tended to have larger Cobb angle of main and secondary curve with worse flexibility of main curve, and CB in Adolescents was better than the other two groups. In the sagittal profile, TK ( F=4.324, P=0.017), LL ( F=4.590, P=0.013), PI ( F=5.501, P=0.006), and PT ( F=3.220, P=0.045) showed statistical difference in the three groups, which were increasing significantly with aging. MRI parameters showed that younger patients were more likely to have a higher degree of cerebellar tonsillar descent (χ 2=18.479, P<0.001) and distended syringomyelia (χ 2=23.074, P=0.003). Conclusion:With aging, Cobb angle of main curve is progressive, and the flexibility is worse, suggesting that early surgical intervention should be performed to reduce the risk of surgery. In addition, cerebellar tonsillar descent and syringomyelia in elderly patients are milder than young patients, indicating that there might be spontaneous remission.

11.
Chinese Journal of Orthopaedic Trauma ; (12): 927-932, 2020.
Article in Chinese | WPRIM | ID: wpr-867957

ABSTRACT

Objective:To conduct an MRI observation on the incidences of ligamentous injuries and fracture morphology in Schatzker type Ⅳ tibial plateau fracture (TPF) and investigate their influence on knee stability.Methods:A retrospective analysis was conducted of the 30 patients with Schatzker type Ⅳ TPF who had undergone surgery at Department of Orthopaedic Trauma, Jiangsu Provincial People's Hospital from January 2010 to December 2019 and whose preoperative X-ray, CT and MRI were available. They were 18 males and 12 females, aged from 22 to 75 years (mean, 45.4 years). They were divided into a dislocation-free group and a dislocation group according to the absence or presence of knee dislocation on their anteroposterior X-ray films. The fracture morphology was assessed on CT according to the modified three-column classification. The incidences of ligamentous injuries [involving anterior cruciate ligament (ACL), posterior cruciate ligament (PCL), medial collateral ligament (MCL), posterolateral complex (PLC) and anterolateral ligament (ALL)] were investigated on MRI. The 2 groups were compared in fracture morphology and incidences of ligamentous injuries. Multiple linear regression (MLR) analysis was used to identify the main factor contributing to preoperative knee dislocation.Results:The dislocation group had 12 patients and the dislocation-free group 18. The fracture involving medial+posteromedial+posterolateral columns was found in 66.7% of the patients (20 cases), and accounted for 83.3% (10 cases) in the dislocation group. The incidence was 96.7% (29 cases) for ACL injury, 43.3% (13 cases) for PCL injury, 70.0% (21 cases) for MCL injury, 90% (27 cases) for PLC injury, 73.3% (22 cases) for ALL injury and 90% (27 cases) for the multiple-ligament disruption. There was a significant difference in the posterolateral column injury between the dislocation-free group [55.6% (10/18)] and the dislocation group [91.7% (11/12)] ( P < 0.05), but there were no significant differences between the 2 group in the injury to any other single ligament or multiple ligaments ( P>0.05). The MLR analysis confirmed that the posterolateral column injury was a risk factor for coronary plane dislocation in Schatzker type Ⅳ TPF( P<0.05). Conclusions:In Schatzker type Ⅳ TPF, the incidences of ligamentous injuries are very high but the fracture of posterolateral column may be the main cause for preoperative knee dislocation in some patients.

12.
Chinese Journal of Orthopaedic Trauma ; (12): 226-230, 2019.
Article in Chinese | WPRIM | ID: wpr-745102

ABSTRACT

Objective To investigate the short-term outcomes and surgical timing in patients who were treated by open reduction and internal fixation (ORIF) for acetabular fractures complicated with injury to lower urinary tract.Methods A retrospective analysis was conducted of the 14 patients with acetabular fracture complicated with lower urinary tract injury who had been treated at Department of Orthopaedics,The First Affiliated Hospital to Nanjing Medical University from January 2011 to September 2017.They were 12 males and 2 females,with an average age of 40.3 years (from 30 to 56 years).According to Letournel-Judet classification,there were one anterior column,2 transverse,3 anterior column plus posterior hemi-transverse,one T-shaped,5 associated both-column and 2 transverse plus posterior wall fractures.The time from injury to surgery ranged from 6.0 to 30.5 days,with an average of 13.4 days.Of the 8 patients with bladder injury undergoing internal fixation of the acetabulum,a modified ilioinguinal approach combined with a Kocher-Langenbeck approach was used in 4,a single modified ilioinguinal approach in 3,and a Stoppa approach combined with an iliac fossa approach in one;of the 6 patients with urethral rupture undergoing internal fixation of the acetabulum,a modified ilioinguinal approach combined with a Kocher-Langenbeck approach was used in 3,a single modified ilioinguinal approach in one,a Kocher-Langenbeck approach in one and a Stoppa approach in one.The fracture union time,outcomes and complications were recorded.Results The mean follow-up time for the 14 patients was 27.2 months (from 12 to 60 months).The fracture union time ranged from 3.0 to 4.5 months,averaging 3.5 months.According to the Majeed's scoring,the pelvic function at the final follow-up was excellent in 7 patients,good in 4,fair in 2 and poor in one;their scores ranged from 30 to 92 points,averging 80 points.No infection or implant failure occurred during follow-ups.Heterotopic ossification at the acetabulum was observed in one patient whose Brooker grading was Ⅳ at the final follow-up.Conclusion The patients with acetabular fracture complicated with lower urethral or bladder injury should be treated with ORIF as soon as possible so that fine clinical outcomes can be achieved.

13.
Chinese Journal of Orthopaedics ; (12): 220-227, 2018.
Article in Chinese | WPRIM | ID: wpr-708529

ABSTRACT

Objective To investigate the natural evolution of postoperative distal adding-on in Lenke 1A and 2A adoles-cent idiopathic scoliosis(AIS)patients,and to explore the risk factors for the progression of distal adding-on.Methods From Ju-ly 2006 to July 2012,a total of 197 AIS patients with Lenke 1A or 2A curves underwent posterior selective thoracic instrumenta-tion and fusion surgery.Among which,44 patients(22.3%)with postoperative distal adding-on were recruited in this study.There were 39 female and 5 male,with an average age of(15.0±2.1)years.The mean Cobb angle of main thoracic curve was 49.3°±9.3°. The first postoperative radiograph indicating distal adding-on and the last follow-up radiograph were compared:make the measure-ment of the disc angle below lowest instrumented vertebra(LIV),and the distance between the vertebra below LIV(LIV+1)and cen-tral sacral vertical line(CSVL).Distal adding-on could be classified into progressive group and non-progressive group according to its natural evolution during follow-up.If the disc angle increased> 5°or the LIV+1-CSVL distance increased>5 mm,the pa-tients were assigned into progressive group; Otherwise, the patients were assigned into non-progressive group. Using Student T test, χ2test or Fisher exact test, the predicted risk factors for progression were screened for further Logistic regression. Results Among the 44 patients enrolled in the study,17 patients(38.6%)had progressive adding-on while 27 patients(61.4%)had non-progressive adding-on.The Risser sign was significantly lower in progressive group than non-progressive group(t=4.399,P<0.001). Besides,more patients had LIV proximal to substantially stable vertebra(SSV)in progressive group than non-progressive group (Fisher exact test value=18.142,P<0.001).The improvement of shoulder imbalance was significantly better in progressive group than non-progressive group(t=3.011, P=0.002). According to Logistic regression, the low Risser sign and LIV proximal to SSV were independent risk factors for progression of distal adding-on.Moreover,the self-image domain of SRS-22 Scores was remark-ably lower in progressive group than non-progressive group(t=2.321,P=0.014).Conclusion Distal adding-on could be classi-fied into progressive group(40%)and non-progressive group(60%)according to its natural evolution.The risk factor for its progres-sion included skeletal immaturity and LIV proximal to SSV.Moreover,the progression of distal adding-on might compensate for the shoulder imbalance during follow-up.

14.
Chinese Journal of Orthopaedic Trauma ; (12): 1029-1035, 2017.
Article in Chinese | WPRIM | ID: wpr-707408

ABSTRACT

Objective To investigate the characteristics,trauma mechanism and treatment of the hyperplantarflexion ankle fracture variant.Methods Between January 2013 and June 2015,125 consecutive patients with ankle fracture received operative treatment.In 18 of them,the ankle joint had been subjected to excessive violence of plantarflexion.They were 6 males and 12 females,aged from 15 to 67 years (average,47.6 years).Their pre-operative ankle radiographs showed sagittal ankle instability and posterior ankle dislocation or subluxation.The injury was complicated with medial malleolus fracture and fibular fracture.Posterolateral approach or posterolateral and posteromedial approach was adopted to treat the patients.Lag screws and anti-glide plates were applied.Results All the 18 patients were followed up for 10 to 25 months (average,16.8 months).All their fractures healed after 11 to 16 weeks (average,13 weeks).Superficial skin necrosis occurred in one patient undergoing dual-plate fixation of the dual malleoli but responded to change of dressing 3 weeks later.No screw loosening,fixation breakage or failure,nonunion or malunion happened.Their final follow-ups showed an average American Orthopedic Foot Ankle Society score of 94.8 ponits (from 76 to 100 points),yielding 13 excellent and 5 good cases (an excellent to good rate of 100%).Conclusions The hyperplantarflextion ankle fracture variant is caused by excessive violence of planter flexion to the ankle joint,with the force on the sagittal plane going from anteriorly to posteriorly,resulting in posterior talar displacement.Its treatment should vary from that for other ankle fractures because it has its own characteristics.Appropriate treatment may lead to satisfactory outcomes.

15.
Chinese Journal of Orthopaedic Trauma ; (12): 729-732, 2016.
Article in Chinese | WPRIM | ID: wpr-497941

ABSTRACT

Abdominal compartment syndrome (ACS) has been increasingly recognized as a cause for significant morbidity and mortality in many diseases.Great advances have been made in many researches that are dedicated to the treatment of ACS.However,in the field of orthopedics,it has still not aroused enough attention from the orthopaedic surgeons,as the occurrence and development of ACS are occult following pelvic fracture trauma.This review introduces the relevant data and researches about ACS resulting from pelvic fractures.

16.
Chinese Journal of Orthopaedic Trauma ; (12): 102-107, 2016.
Article in Chinese | WPRIM | ID: wpr-489202

ABSTRACT

Objective To discuss the efficacy of the modified subinguinal approach for the treatment of acetabular anterior column and wall fractures.Methods A clinical retrospective study was performed of the 5 patients who had been treated at our department from January 2011 to April 2015 for acetabular anterior column and wall fractures and conformed to follow-ups.They were 3 males and 2 females,aged from 19 to 45 years (mean,35.6 years).The duration from injury to surgery averaged 7.6 days (range,from 4 to 10 days).The subinguinal ligament approach was adopted in the 5 cases,through which the inguinal ligament was preserved intact after sharp dissection of its insertion at the anterior superior iliac spine and medial-upper advancement,the advantage of expanded exposure of the first window by the Farid sub-ilioinguinal approach was retained,and additional lesions due to iliac osteotomy were avoided.The operation time,intraoperative bleeding,postoperative reduction and time for fracture union were documented.Merle d' Aubigne and Postel scoring system was used to assess the hip joint function of the affected limb at the final follow-ups.Results The operation time ranged from 110 to 150 minutes (mean,125 minutes);the intraoperative bleeding ranged from 415 to 550 mL (mean,450.2 mL).By the Matta's criteria,the postoperative reduction was rated as excellent in 4 cases and good in one.The 5 patients were followed up for an average of 32.4 months (range,from 6 to 43 months).Their fractures united clinically after an average of 4.6 months (range,from 3 to 6 months).The hip joint function was rated as excellent in 2 and good in 3 by the Merle d' Aubigne and Postel scoring system at the final follow-ups.Follow-ups observed no serious complications like inguinal hernia,internal fixation failure,myositis ossificans,or avascular necrosis of the femoral head.Conclusions As the modified subinguinal approach can provide a broad surgical exposure,lead to minimal injury to the inguinal ligament,avoid lesions caused by iliac osteotomy,and decrease operation time and bleeding,it may be a better approach for acetabular anterior column and wall fractures.

17.
West China Journal of Stomatology ; (6): 443-447, 2016.
Article in Chinese | WPRIM | ID: wpr-317788

ABSTRACT

<p><b>OBJECTIVE</b>To construct a cell line of oral mucosa epithelial cells that stably express human telomerase reverse transcriptase (hTERT) by lentiviral vectors, approaches for the establishment of stable and efficient immortalized oral mucosa epithelial cell lines were explored.</p><p><b>METHODS</b>Whole RNA was extracted from 293T cells. The hTERT gene was amplified by polymerase chain reaction (PCR) and cloned into the lentiviral vector as pLVX-puro-hTERT. The lentivirus particles were successfully packaged and used to infect primary oral epithelial cells. The positive cell clones were selected by puromycin. Finally, the expression of hTERT was examined by real-time fluorescent quantitative PCR (qRT-PCR) and Western blot analysis.</p><p><b>RESULTS</b>The sequencing results confirmed the construction of the recombinant lentivirus pLVX-puro-hTERT. The morphology of infected cells was similar to that of normal oral mucosal epithelial cells, with a cobble stone-like appearance. The qRT-PCR and Western blot results showed that hTERT was overexpressed in infected cells compared with the normal group (P<0.05).</p><p><b>CONCLUSIONS</b>The oral epithelial cell line with stable expression of hTERT was successfully established by the lentivirus, which provides an experimental basis for the establishment of a highly efficient and stable oral epithelial immortalized cell line.</p>


Subject(s)
Humans , HEK293 Cells , Lentivirus , Mouth , Mucous Membrane , Real-Time Polymerase Chain Reaction , Telomerase
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