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Chinese Journal of Biochemical Pharmaceutics ; (6): 65-67, 2014.
Article in Chinese | WPRIM | ID: wpr-452135

ABSTRACT

Objective To investigate Anti-aging function of Placenta freeze-dried powder on mice. Method 2 month old female-KM mice were divided into five groups:normal control group, aging model group, positive control group, placenta freeze-dried powder low dose group and high dose group. Except normal control group,the rest of groups were treated with method of subcutaneous continuous injection of D-galactose for 42 days in order to establish mice aging model. Meanwhile, the corresponding drugs, via intragastric administration, were ingested by different treated groups and observe the change of immunity and physiological regulation related in mice. Results Compared with aging model group, thymus index and spleen index in placenta freeze-dried powder low dose group and high dose group increased obviously (P<0.05), content of MDA in brain decreased significantly (P<0.05), proportion of CD 4 and CD 8 lymphocyte in total lymphocyte, female hormone(P and E 2), IgG and haemopoietic factor in peripheral blood increased remarkably. Conclusion Placenta freeze-dried powder could slow the aging process on mice via immunity enhancement and improving physical regulation.

2.
Chinese Journal of Hepatology ; (12): 519-524, 2014.
Article in Chinese | WPRIM | ID: wpr-314010

ABSTRACT

<p><b>OBJECTIVE</b>To investigate changes in gene expression that occur upon treatment with human umbilical cord mesenchymal stem cells (UC-MSCs) for hepatic cirrhosis using a rat model system.</p><p><b>METHODS</b>Hepatic cirrhosis was induced in Sprague-Dawley rats by subcutaneous injection of carbon tetrachloride and oral administration of alcohol.UC-MSCs were isolated from human umbilical cord and the cells' immunophenotype and differentiation towards osteogenic and adipogenic lineages were confirmed.The UC-MSC sample or vehicle alone (phosphate buffered saline, PBS) was transplanted by intravenous injection.Histopathological staining and serological testing were used to compare the liver morphology and function among the different groups.The gene expression in the PBS group and UC-MSC group were detected by gene microarray and differences between the groups were statistically analyzed by t-test.</p><p><b>RESULTS</b>Transplantation of the UC-MSCs improved liver function in the hepatic cirrhosis rats.Comparison of the gene expression profiles of the PBS group and the UC-MSC group showed that the latter had up-regulation of the genes related to the complement and coagulation cascades and down-regulation of the genes related to cell proliferation, cell cycle, and collagen synthesis.</p><p><b>CONCLUSION</b>UC-MSC therapy might improve liver function in cirrhosis by increasing the expression of genes related to the complement and coagulation cascades and by decreasing genes involved in cell proliferation and collagen deposition.</p>


Subject(s)
Animals , Humans , Rats , Carbon Tetrachloride , Cell Differentiation , Cell Proliferation , Down-Regulation , Gene Expression Profiling , Liver Cirrhosis , Mesenchymal Stem Cells , Rats, Sprague-Dawley , Transcriptome , Umbilical Cord , Up-Regulation
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