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Article in Chinese | WPRIM | ID: wpr-479816


Most of the maxillary first premolar are single-rooted,there are also two-rooted and three-rooted ones.A case of three-rooted maxillary first premolar was diagnosed by CBCT.CBCT has the advantage in the observation of the variation root number,root canal bending and the variation of root canal morphology.

Article in Chinese | WPRIM | ID: wpr-602113


Objective To explore the effect of decrease of Survivin expression on the proliferation and apoptosis of the naso-pharyngeal carcinoma cells.Methods To observe the effect of decrease of Survivin expression on nasopharyngeal carcinoma cell morphology using RNAi technology combined with RT-PCR and Western blot.Cell proliferation and apoptosis were de-tected by MTT and TUNEL assay.The expression of PARP,Bcl-2 and Bax was detected by Western blot.Results RT-PCR result showed the survivin mRNA expression in Surviving-siRNA groups was downregulated (about 0.26±0.02)and the inhibition ratio was 43.7% (P<0.05).MTT result showed cell proliferation rates were significantly different between 24,48 and 72 h after transfection.The cell inhibition rates were 21.9%,37.1% and 29.6%,respectively (P<0.05).West-ern blot result showed the Survivin protein expression in Survivin-siRNA2 groups was downregalated and the relative ex-pression level was reduced by 57% (P<0.05).TUNEL assay showed that cell apoptosis rate was increased obviously in surviving-siRNA groups.The expression of PARP (89KD)and Bax wasupregulated (3.9 and 2.4 fold change)and the ex-pression of Bcl-2 was downregulated (0.3 fold change).The phosphorylation of AKT was inhibited when Survivin was down-regulated (about reduced by 5 7%).Conclusion Silencing Survivin gene by siRNA can inhibit the proliferation of Na-sopharyngeal carcinoma cells and induce apoptosis.Survivin may become a potential gene for therapy target of nasopharynge-al carcinoma.