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1.
Chinese Journal of Neurology ; (12): 28-33, 2021.
Article in Chinese | WPRIM | ID: wpr-885397

ABSTRACT

Objective:To explore the electro-clinical characteristics of Jeavons syndrome complicated with non-convulsive status epilepticus (NCSE).Methods:Three patients of Jeavons syndrome complicated with NCSE during electroencephalogram (EEG) monitoring in the EEG Monitoring Center, Department of Neurology, Xijing Hospital in 2018 were studied and followed up.Results:Among the three patients, there are two female patients, one male patient, aged 12 to 24 years. Generalized spike-wave discharges and impaired consciousness were recorded during video-EEG monitoring, which lasted for 8 minutes to 6.5 hours and evolved in generalized tonic clonic seizure (GTCS), and did not recur after receiving correct anti-epileptic drugs.Conclusion:NCSE can also occur in Jeavons syndrome, and NCSE in patients with Jeavons syndrome ends with GTCS, suggesting that early detection and timely termination of NCSE in patients with Jeavons syndrome may avoid the occurrence of GTCS.

2.
Chinese Journal of Neurology ; (12): 22-27, 2021.
Article in Chinese | WPRIM | ID: wpr-885385

ABSTRACT

Objective:To investigate the clinical characteristics and electroencephalogram (EEG) of epilepsy patients with breach rhythm, improve clinical understanding of breach rhythm and avoid over-interpretation.Methods:Twelve epilepsy patients with breach rhythm who visited the Department of Neurology, Xijing Hospital, the Air Force Military Medical University from January 2016 to January 2017 were collected retrospectively. The clinical data, including etiology, clinical manifestations, EEG features and prognosis were summarized, and outpatient and telephone follow-up was performed for at least three years.Results:The clinical data of 12 patients with epilepsy with breach rhythm were collected, including eight males and four females, aged 36-78 years. After analysis, it was found that brain trauma was the most common cause of breach rhythm. Among them, two cases of skull defect were not repaired, eight cases were repaired with skull titanium mesh, one case was repaired with skull polymethylmethacrylate, and one case was repaired with skull polyetheretherketone. The distribution of the breach rhythm in 12 patients was consistent with the abnormal area of the skull. The breach rhythm can be expressed as high amplitude and fast frequency, or low amplitude and slow frequency and appear individually (similar to sharp waves, spikes). On the basis of pleomorphic slow waves, 10 patients were mixed with sharp waves and spike waves, and imaging confirmed that they had brain damage in corresponding parts. All of the 12 patients had a history of seizures, with tonic-clonic seizures and (or) focal seizures being the most common. Three patients with breach rhythm had no clinical seizures for more than five years, and had been taking antiepileptic drugs for epileptic spikes on EEG, and they were given reduction and discontinuation of the drugs and were seizure-free for three years during follow up.Conclusions:Skull repair is a common cause of breach rhythm, and repair materials with different resistances cause different waveforms and frequencies. Breach rhythm, epileptiform discharge and other pathological slow-wave activities can exist at the same time. Breach rhythm is a benign variant phenomenon which needs no special treatment.

3.
Article in Chinese | WPRIM | ID: wpr-883193

ABSTRACT

Objective:To explore the etiology and characteristics of chronic wet cough in children in Qingdao.Methods:Patients with chronic wet cough treated at respiratory clinic of the Women and Children′s Hospital Affiliated to Qingdao University from July 2018 to June 2019 were included in this study.After three-month follow-up, the etiological data was analyzed.Results:(1)A total of 213 children were included, ranging in age from 1 month to 14 years old, including 38 cases of 1 month~1 year old, 47 cases of 1~3 years old, 87 cases of 3~6 years old, and 41 cases of 6~14 years old.The median age was 4.7 years.The top four causes of chronic wet cough in children were upper airway cough syndrome(33.8%), protracted bacterial bronchitis(20.7%), asthma with upper airway cough syndrome(15.5%), and asthma with infection(10.8%). Other causes were postinfection cough, pertussis syndrome, bronchiectasis, gastroesophageal reflux, bronchial foreign body, abnormal airway development, cystic fibrosis and so on.(2)The first cause of chronic wet cough in different age groups: 1 month to 3 years old group was protracted bacterial bronchitis; 3 to 14 years old group was upper airway cough syndrome.(3)The causes of chronic wet cough showed seasonal differences.Upper airway cough syndrome and cough after infection had a more balanced incidence throughout the year; protracted bacterial bronchitis and pertussis syndrome were common in winter; asthma with upper airway cough syndrome and asthma with infection were common in spring and autumn.Conclusion:Upper airway cough syndrome, protracted bacterial bronchitis, asthma with upper airway cough syndrome, and asthma with infection are the 4 leading causes for children with chronic wet cough in Qingdao.The causes of chronic wet cough have age and seasonal differences.

4.
Chinese Journal of Biotechnology ; (12): 1324-1333, 2021.
Article in Chinese | WPRIM | ID: wpr-878634

ABSTRACT

Photoperiod plays an important role in transformation from vegetative growth to reproductive growth in plants. CONSTANS (CO), as a unique gene in the photoperiod pathway, responds to changes of day length to initiate flowering in the plant. In this study, the expression level of FaCONSTANS (FaCO) gene under long-day, short-day, continuous light and continuous darkness conditions was analyzed by real-time quantitative PCR. We constructed the over-expression vector p1300-FaCO and infected into Arabidopsis thaliana by Agrobacterium-mediated method. We constructed the silencing vector p1300-FaCO-RNAi and infected into Festuca arundinacea by Agrobacterium-mediated method. The expression of FaCO gene was regulated by photoperiod. The over-expression of FaCO promoted flowering in wild type of Arabidopsis thaliana under long day condition and rescued the late flowering phenotype in co-2 mutant of Arabidopsis thaliana. Silencing FaCO gene in Festuca arundinacea by RNAi showed late-flowering phenotype or always kept in the vegetative growth stage. Our understanding the function of FaCO in flowering regulation will help further understand biological function of this gene in Festuca arundinacea.


Subject(s)
Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Festuca/metabolism , Flowers/genetics , Gene Expression Regulation, Plant , Photoperiod
5.
Article in Chinese | WPRIM | ID: wpr-912358

ABSTRACT

Objective:To dynamically observe the effect of N-acetylserotonin (NAS) on the expression of tumor necrosis factor-α (TNF-α) protein in retina of retinal ischemia reperfusion injury (RIRI) rats, and to explore the mechanism.Methods:By using random number table method, 90 healthy male Sprague-Dawley rats were divided into sham operation group ( n=10), RIRI group ( n=40), and NAS group ( n=40). The right eye was as the experimental eye. In the RIRI group and NAS group, the anterior chamber high intraocular pressure method was used to establish the RIRI model. In the NAS group, 10 mg/kg NAS was injected intraperitoneally before modeling and 30 minutes after modeling. At 6, 12, 24, 72 h after modeling, hematoxylin-eosin staining was used to observe the pathological changes of the retina, and the retinal ganglion cells (RGC) were counted. Each group was detected by immunohistochemical staining and Western blot about the relative expression of TNF-α, nuclear factor E2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) protein in the rat retina. Oneway analysis of variance was used for differences between groups. The general linear regression method was used to analyze the correlation between the relative expression changes of TNF-α protein and the changes of Nrf2 and HO-1 protein expression after NAS intervention. Results:Optical microscope observation revealed that the retinal edema of rats in the RIRI group was observed at 6, 12, and 24 h after modeling; the thickness of the retina in the NAS group was significantly thinner than that in the RIRI group, and the difference was statistically significant ( F=9.645, 477.150, 2.432; P<0.01). At 6, 12, 24, and 72 h after modeling, the retinal RGC counts in the NAS group were significantly higher than those in the RIRI group, and the difference was statistically significant ( F=12.225, 12.848, 117.655, 306.394; P<0.05). The results of immunohistochemical staining and Western blot showed that 6 h after modeling, the relative expression of TNF-α protein in the retina of the RIRI group increased significantly compared with that in the sham operation group, reaching a higher level at 12 h, and decreased at 24 and 72 h. But all were significantly higher than the sham operation group, the difference was statistically significant (immunohistochemical staining: F=105.893, 1 356.076, 434.026, 337.351; P<0.01; Western blot: F=92.906, 534.948, 327.600, 385.324; P<0.01). At different time points after modeling, the relative expression of TNF-α protein in the retina of the NAS group was significantly lower than that of the RIRI group (immunohistochemical staining: F=15.408, 570.482, 21.070, 13.767; P<0.05; Western blot: F=12.618, 115.735, 13.176, 111.108; P<0.05), but still higher than the sham operation group (immunohistochemical staining: F=40.709, 151.032, 156.321, 216.035; P<0.01; Western blot: F=33.943, 79.729, 74.057, 64.488; P<0.01), the difference was statistically significant; 12 h after modeling, Nrf2 in the retina of the NAS group (immunohistochemical staining: F=51.122, P<0.05; Western blot: F=33.972, P<0.05), HO-1 (immunohistochemical staining: F=30.750, P<0.05; Western blot: F=18.283, P<0.05) protein relative expression was significantly higher than that of RIRI group, and the differences were statistically significant. The results of linear regression analysis showed that the difference in the number of TNF-α + cells in the RIRI group and the NAS group was negatively correlated with the difference in the number of Nrf2 + and HO-1 + cells ( r 2=0.923, 0.936; P<0.01). Conclusions:NAS can inhibit the expression of TNF-α protein in the retina of RIRI rats and reduce RIRI. The mechanism may be related to the Nrf2/HO-1 pathway.

6.
Chinese Journal of Neurology ; (12): 1249-1255, 2021.
Article in Chinese | WPRIM | ID: wpr-911862

ABSTRACT

Objective:To discuss the clinical and electrophysiological characteristics of familial cortical myoclonic tremor with epilepsy (FCMTE) with fixation-off sensitivity (FOS).Methods:The clinical and electrophysiological characteristics of four patients diagnosed as FCMTE with FOS in the Electroencephalography (EEG) Monitoring Center of Xijing Hospital from May 2016 to December 2017 were studied and followed up.Results:The four patients were all female. The age was ranged from 29 to 67 years. The course was from six to 30 years, and the follow-up time was at least two years. The tremors and jerks occurred to the four patients frequently when the eyes were closed, which prevented their falling a sleep, and three of them had generalized tonic-clonic seizure occasionally. The FOS was monitored in the all four patients, and the photosensitivity occured to the three of them.Conclusions:The fixation-off sensitive trail during EEG monitoring is helpful to find the FCMTE with FOS. It is necessary to determine the potential clinical significance of FOS and photosensitivity coexisting in patients with FCMTE.

7.
Chinese Journal of Endemiology ; (12): 712-717, 2021.
Article in Chinese | WPRIM | ID: wpr-909083

ABSTRACT

Objective:An autoregressive integrated moving average (ARIMA) model was used to predict the number of monthly reported cases of schistosomiasis in China (excluding Hong Kong, Macao and Taiwan), so as to provide a scientific basis for prevention and control of schistosomiasis.Methods:Using ARIMA model, taking the time series of monthly reported cases of schistosomiasis in China from January 2009 to December 2018 as the training set, after stabilizing analysis with R 3.6.2 software, ARIMA models were selected by using screening parameters such as akaike information criterion and bayesian information criterion. Taking the number of monthly reported cases of schistosomiasis in China from January to December 2019 as the test set for verification and monthly optimization, an optimal ARIMA model was obtained. The prediction effect of the optimal ARIMA model was verified by the number of monthly reported cases of schistosomiasis in China from January 2019 to October 2020.Results:Based on the data of monthly reported cases of schistosomiasis in China from January 2009 to December 2018, four ARIMA models were obtained, namely ARIMA(2,0,2)(1,0,1)[12], ARIMA(2,0,2)(0,0,1)[12], ARIMA(2,0,2)(1,0,0)[12] and ARIMA(2,0,2). By comparing the actual number of cases from January to December 2019 with the predicted values of the four ARIMA models, the optimal prediction model of monthly reported cases of schistosomiasis was ARIMA(2,0,2)(1,0,1)[12], and the mean relative error of the prediction was 0.51%.Conclusions:The ARIMA model constructed in this study has high accuracy and is suitable for short-term prediction and analysis of the number of schistosomiasis cases in China. It can provide data support for prevention and control of the disease, and has certain practical guiding significance.

8.
Article in Chinese | WPRIM | ID: wpr-908402

ABSTRACT

Objective:To summarize the clinical characteristics of 102 children with protracted bacterial bronchitis in Qingdao area.Methods:One hundred and two children with protracted bacterial bronchitis treated at respiratory clinic of Women and Children′s Hospital affiliated to Qingdao University from January 2016 to March 2021 were included in this study.The clinical data, age and seasonal distribution, etiology, clinical manifestations, high-resolution CT of the lungs, characteristics of lung function, bronchoscopy and treatment prognosis were retrospectively analized.Results:The top three pathogens of protracted bacterial bronchitis in 102 children (55 boys, 47 girls, median age: 1.7 (0.8, 4.2)years were Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis, with frequency of 12.75%, 10.78%, and 6.86%, respectively.The onset season was mainly autumn and winter, with a total of 73 (71.57%) cases.The main clinical manifestations were wet cough and (or) wheezing, and the cough was not divided between day and night.CT of the lungs showed thickening of the bronchial wall in 16(15.69%) cases and uneven ventilation in 5(4.90%) cases.Twenty-three (22.55%) cases had abnormal lung function.Fifty-two (50.98%) cases underwent bronchoscopy, of which 33(32.35%) cases had multiple lung segmental purulent changes under the microscope.After standard anti-infection, bronchoscopy and alveolar lavage, expectorant and physical therapy, the prognosis was mostly good.Conclusion:The clinical manifestations of protracted bacterial bronchitis in children have no obvious specificity.Early diagnosis, identification of the cause and comprehensive management are critical to its prognosis.

9.
Article in Chinese | WPRIM | ID: wpr-907927

ABSTRACT

Objective:To investigate the effect and mechanism of NACK knockdown on the proliferation and apoptosis of T-cell acute lymphoblastic leukemia (T-ALL) Jurkat cells. Methods:Lentivirus transfection technology was used to transfect Jurkat cells and knock down NACK gene.Real time fluorescent quantitative PCR and Western blot were used to detect the silencing efficiency of NACK gene.CCK-8 method and flow cytometry were used to detect the effects of NACK knockdown on the proliferation and apoptosis of Jurkat cells.The expressions of protein related with Notch1 pathway, such as Hes1 and c-Myc, were detected by Western blot. Results:After NACK-shRNA was successfully transfected into Jurkat cells by lentiviral vector, the expression of NACK mRNA and protein was reduced signi-ficantly ( P<0.05). Compared with the negative control group and the blank control group, the CCK-8 method showed that the cell proliferation in the experimental group was significantly inhibited [The inhibition rates of cell proliferation in the experimental group, negative control group and blank control group were (37.27±4.48)%, (4.25±2.10)% and (2.43±1.40)%, respectively]( F=132.640, P<0.05), and the flow cytometry test showed that the apoptosis in the experimental group increased significantly [The apoptosis rates of experimental group, negative control group and blank control group were (26.38±3.03)%, (6.07±2.61)% and (3.40±1.98)%, respectively]( F=90.534, P<0.05). Western blot results confirmed that the expression of Notch1 pathway-related proteins Hes1 and c-Myc was down-regulated compared with the negative control group and the blank control group, and the difference was statistically significant ( P<0.05). Conclusions:Targeting silent NACK can down-regulate the expression of Notch1 pathway-related proteins, which leads to the inhibition of Jurkat cell proliferation and increased apoptosis, thereby exerting its anti-T-ALL effect.

10.
Article in Chinese | WPRIM | ID: wpr-907571

ABSTRACT

Objective:To compare the differences in population distribution and prognosis of patients with nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy (IMRT) in T staging of the Union for International Cancer Control (UICC) 7th edition and UICC 8th edition, and to analyze the prognostic factors in patients with NPC.Methods:The clinicopathologic date of 184 patients with newly diagnosed NPC treated with IMRT at the Department of Radiation Oncology of Weifang People′s Hospital of Shandong Province from June 1, 2005 to December 31, 2017 were retrospectively analyzed. All patients were restaged according to the 7th and 8th edition of the UICC staging system. The distribution of T staging of patients in the two staging systems was analyzed, and the consistency of the two staging systems was compared using the Kappa consistency test. Kaplan-Meier method was used for survival analysis, and log-rank test was used to compare the prognostic differences among T stages. Cox regression model was used to analyze the prognostic factors of patients with NPC.Results:Of all 184 patients with NPC, stage T 1, T 2, T 3 and T 4 respectively accounted for 18.5% (34/184), 16.8% (31/184), 15.2% (28/184) and 49.5% (91/184) according to the 7th edition UICC staging system. However, stage T 1, T 2, T 3 and T 4 respectively accounted for 18.5% (34/184), 34.2% (63/184), 30.4% (56/184) and 16.8% (31/184) according to the 8th edition UICC staging system. The T staging population distribution of the two staging systems showed moderate consistency (Kappa=0.58). There was a statistically significant difference in overall survival (OS) among patients with stage T 1, T 2, T 3, T 4 according to the 7th edition UICC staging system ( χ2=10.606, P=0.014). There were statistically significant differences in OS between stage T 1 and stage T 2, T 3, T 4 ( χ2=4.866, P=0.027; χ2=11.965, P=0.001; χ2=4.351, P=0.037). The OS curves of stage T 2 and T 4 could not be separated. Moreover, the OS curves of stage T 3 and T 4 were distributed in reverse order. There was a statistically significant difference in OS among patients with stage T 1, T 2, T 3, T 4 according to the 8th edition staging system ( χ2=8.663, P=0.034). There were statistically significant differences in OS between stage T 1 and stage T 3, T 4( χ2=8.746, P=0.003; χ2=7.580, P=0.006). The OS curves of stage T 1 to T 4 were distributed in order, but the curves of stage T 3 and T 4 could not be separated. There was a statistically significant difference in progression-free survival (PFS) among patients with stage T 1, T 2, T 3, T 4 according to the 7th edition UICC staging system ( χ2=11.289, P=0.010). There were statistically significant differences in PFS between stage T 1 and stage T 2, T 3, T 4 ( χ2=8.209, P=0.004; χ2=13.302, P<0.001; χ2=6.550, P=0.010). The PFS curves of stage T 2 and T 4 could not be separated. Moreover, the PFS curves of stage T 3 and T 4 were distributed in reverse order. There was a statistically significant difference in PFS among patients with stage T 1, T 2, T 3, T 4 according to the 8th edition staging system ( χ2=12.074, P=0.007). There were statistically significant differences in PFS between stage T 1 and stage T 2, T 3, T 4( χ2=5.182, P=0.023; χ2=11.217, P=0.001; χ2=10.174, P=0.001). The PFS curves of stage T 1 to T 4 were distributed in order, but the curves of stage T 3 and T 4 could not be separated. The results of Cox multivariate analysis showed that T staging of both staging systems were the independent prognostic factors of the OS ( P=0.013; P=0.026) and PFS ( P=0.031; P=0.012). However, T staging of the two editions were not the independent prognostic factors of the local recurrence-free survival (LRFS) ( P=0.351; P=0.167) and distant metastasis-free survival (DMFS) ( P=0.059; P=0.052). The age was the independent prognostic factor of the OS ( HR=2.70, 95% CI: 1.53-4.76, P=0.001; HR=2.74, 95% CI: 1.55-4.84, P=0.001), PFS ( HR=2.72, 95% CI: 1.46-5.08, P=0.002; HR=2.94, 95% CI: 1.57-5.52, P=0.001), LRFS ( HR=5.87, 95% CI: 1.62-21.27, P=0.007; HR=6.02, 95% CI: 1.61-22.49, P=0.008) and DMFS ( HR=2.40, 95% CI: 1.22-4.72, P=0.011; HR=2.63, 95% CI: 1.34-5.18, P=0.005). N staging was the independent prognostic factor of the OS ( P=0.031; P=0.028). Conclusion:The T staging population distribution of the 7th and 8th edition UICC staging system had moderate consistency, and the T staging of the 8th edition is more advantageous in predicting the prognosis of OS and PFS. In both editions, T staging is an independent prognostic factor for OS and PFS.

11.
Chinese Journal of School Health ; (12): 1852-1854, 2021.
Article in Chinese | WPRIM | ID: wpr-907076

ABSTRACT

Objective@#To assess physical activity level and characteristics in elementary physical education (P.E.) class among different weight status and grades students.@*Methods@#ActiGraphGT3X was used to measure physical activity of 60 students in grade 1, 2, and 4 during three P.E. classes. BMI was calculated according to the student s height and weight in the latest physical fitness test results, and the weight status was divided according to the national Physical Fitness Standards for Students in different grades of elementary schools. Independent sample t test and analysis of variance were used to explore the difference of physical activity.@*Results@#The proportion of moderate to high intensity physical activity (MVPA) in elementary school P.E. class was 21.9% (8.8 min). The proportion of MVPA in P.E. classes and the number of steps in normal weight students were 25.4% and (1 247.0± 326.3 ), respectively, which were significantly higher than those of underweight students, but not significantly different from those of overweight students. The MVPA of male and female PE classes in the second grade (27.0%, 26.4%) was significantly higher than that in the first grade, but there was no significant difference compared with that in the fourth grade ( P >0.05).@*Conclusion@#Moderate to high intensity physical activity is low in P.E. class in elementary school. Improving teachers awareness of promoting physical activity in PE class will benefit the MVPA in students.

12.
Chinese Journal of School Health ; (12): 1852-1854, 2021.
Article in Chinese | WPRIM | ID: wpr-907075

ABSTRACT

Objective@#To assess physical activity level and characteristics in elementary physical education (P.E.) class among different weight status and grades students.@*Methods@#ActiGraphGT3X was used to measure physical activity of 60 students in grade 1, 2, and 4 during three P.E. classes. BMI was calculated according to the student s height and weight in the latest physical fitness test results, and the weight status was divided according to the national Physical Fitness Standards for Students in different grades of elementary schools. Independent sample t test and analysis of variance were used to explore the difference of physical activity.@*Results@#The proportion of moderate to high intensity physical activity (MVPA) in elementary school P.E. class was 21.9% (8.8 min). The proportion of MVPA in P.E. classes and the number of steps in normal weight students were 25.4% and (1 247.0± 326.3 ), respectively, which were significantly higher than those of underweight students, but not significantly different from those of overweight students. The MVPA of male and female PE classes in the second grade (27.0%, 26.4%) was significantly higher than that in the first grade, but there was no significant difference compared with that in the fourth grade ( P >0.05).@*Conclusion@#Moderate to high intensity physical activity is low in P.E. class in elementary school. Improving teachers awareness of promoting physical activity in PE class will benefit the MVPA in students.

13.
China Pharmacy ; (12): 2868-2874, 2021.
Article in Chinese | WPRIM | ID: wpr-906653

ABSTRACT

OBJECTIVE:To study the improvement eff ects and p otential mechanism of chelidonine on CCl 4-induced hepatic fibrosis model rats. METHODS :According to the random number table method ,a total of 48 rats were randomly divided into normal control group ,model group ,chelidonine low-dose ,middle-dose and high-dose groups (0.125,0.25,0.50 mg/kg),positive control group (Liver-protecting tablet ,0.42 g/kg),with 8 rats in each group. Except for normal control group ,other groups were given CCl 4-olive oil solution intraperitoneally for 8 weeks to induce hepatic fibrosis model. From the fifth week of modeling , normal control group and model group were given water intragastrically ;administration groups were given relevant medicine intragastrically,once a day ,for consecutive 10 weeks. After last intragastric administration ,hepatic index of rats was calculated. The levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT)and hyaluronic acid (HA)in serum and the level of hydroxyproline (Hyp)in liver tissue were determined. The staining of collagen fibrin in rat liver was observed. The protein expression of α-smooth muscle actin (α-SMA),microtubule-associated protein 1 light chain 3(LC3)and p 62 as well as the phosphorylation level of phosphoinositide 3 kinase(PI3K),protein kinase B (Akt)and mammalian target of rapamycin (mTOR)in liver tissue were determined ;mRNA expression corresponding to above protein were also determined. RESULTS :Compared with normal control group ,the hepatic index ,the serum levels of AST ,ALT,HA and Hyp ,the percentage of positive staining area for collagen fibrin ,the mRNA and protein expression of α-SMA and LC 3- Ⅱ were increased significantly (P<0.05). Protein expression of p 62,phosphorylation levels of PI 3K,Akt and mTOR as well as mRNA expression of p 62,PI3K,Akt and mTOR were significantly down-regulated (P<0.05). Compared with model group ,phosphorylation levels of PI 3K and mTOR were decreased significantly in chelidonine low-dose group (P<0.05). The changes of above indexes in chelidonine middle-dose and high-dose groups (except for liver index , HA level in middle-dose group ) were reversed significantly (P<0.05). CONCLUSIONS:Chelidonine can attenuate CCl 4-induced liver fibrosis in rats ;the mechanism of it may be associated with activating PI 3K/Akt/mTOR signaling pathway and inhibiting autophagy.

14.
Article in Chinese | WPRIM | ID: wpr-865000

ABSTRACT

Objective:To summarize the characteristics of chest CT imaging and prognosis of children with necrotizing pneumonia (NP) after flexible bronchoscopy, in order to improve the clinician′s diagnosis and treatment of this disease.Methods:The clinical information, imaging features of the sixty-six patients suffering from NP, who were diagnosed and treated in Qingdao Women and Children′s Hospital from September 2015 to April 2019, were retrospectively analyzed. Fourty-six cases who were treated with flexible bronchoscopy alveolar lavage(treatment group) were comparative analyzed with the left 20 cases who were not treated with flexible bronchoscopy alveolar lavage(control group).Results:In treatment group, all patients appered lung consolidation at early stages, and multiple balloon chambers emerged in consolidation shadows in late period. There was no statistic difference in basic information, clinical manifestations, laboratory examinations and etiology between the control group and treatment group( P>0.05). The treatment group had a lower incidence of pulmonary complications and a higher lung shadow full absorption rate, which showed the statistical difference( P<0.05). Conclusion:The flexible bronchoscopy alveolar lavage plays an important role in the process of treating the children with NP, and chest CT provides reliable imaging evidence in the early diagnosis and prognosis of the NP children.

15.
Chinese Journal of Microsurgery ; (6): 459-463, 2020.
Article in Chinese | WPRIM | ID: wpr-871562

ABSTRACT

Objective:To explore the application effect of 3D printing technology in comprehensive reconstruction of thumb and finger.Methods:From January, 2018 to January, 2020, 67 patients with 84 thumbs and fingers defects were selected, which were 37 thumbs and 47 fingers, and 45 of I-III degree and 39 of IV-VI degree. The method of operation was comprehensive reconstruction of thumbs and fingers assisted by 3D printing technology. The patient's hands and feet were scanned with CT and 3D modeling, and the 1∶1 model was derived. The skin model and bone model of thumb and finger defect were printed. Put the skin model on the great toes, and design the shape of the nail flap to be cut. Then the flap was spread in the groin area to design the perforating branch flap of the superficial circumflex iliac artery. The interphalangeal joint or metatarsophalangeal joint of the second toe was cut off according to the skeleton model (when the length of the bone joint was not enough, the iliac bone strip was taken to be connected in series). The effect of operation was observed in outpatient.Results:All of the 84 thumbs and fingers survived and were followed-up for 3 to 24 months. The appearance of the reconstructed thumbs and fingers was similar to that of the normal ones, with good texture and elasticity. The nail was smooth and glossy. The sensory recovery was S 3+, and the TPD was 4-6 mm. According to the Evaluation Standard of Finger Replantation and Reconstruction of Hand Surgery Society of Chinese Medical Association, 70 thumbs and fingers were excellent and 14 thumbs and fingers were good. In 64 thumbs and fingers, all toes were preserved in the donor area, and the color and texture of the flap were satisfactory, which did not affect walking, running and jumping, and had no walking pain. Conclusion:With the help of 3D printing technology, the tissues can be cut more accurately during the thumb and finger comprehensive reconstruction, which not only improves the beauty of reconstructed thumbs and fingers, but also avoids unnecessary trauma and improves the satisfaction of patients.

16.
Chinese Journal of Neurology ; (12): 410-415, 2020.
Article in Chinese | WPRIM | ID: wpr-870836

ABSTRACT

Objective:To discuss the clinical and electrophysiological characteristics of propriospinal myoclonus (PSM).Methods:The clinical and electrophysiological characteristics of four patients diagnosed as PSM in the Electroencephalography Monitoring Center, Department of Neurology, Xijing Hospital, the Air Force Military Medical University from April 2018 to July 2019 were studied. All patients were accorded with diagnostic criteria of PSM that international classification of sleep disorders-3 edition recommended and were followed up.Results:There were three males and one female in the four patients. The age of onset was ranged from 43 to 55 years. The course was from eight months to three years, and the follow-up time was from three months to one year. The clinical features of the four patients were characteristically paroxysmal tic or shaking of the neck, trunk or limbs, with short duration and great frequency. All patients accepted 24-hour video electroencephalography monitoring. No epileptic discharge was recorded during the monitoring. The burst activity of deltoid, quadriceps or rectus abdominis muscle was monitored by surface electromyography at the onset of myoclonus. All patients were treated with clonazepam. Three patients had an obvious curative effect and one patient had no effect.Conclusions:The clinical manifestation of PSM is similar to seizures. There is no epileptic discharge, and only the burst activity of muscles is monitored at the onset. Most patients have significant effect on clonazepam.

17.
Article in Chinese | WPRIM | ID: wpr-825690

ABSTRACT

Objective To analyze the main causes of death among elderly residents aged 60 and above in Huangdu, Jiading District, Shanghai, and to provide evidence for disease prevention and control of the elderly. Methods The mortality rate, potential years of life lost (PYLL), years of life lost rate (PYLLR), and average years of life lost (AYLL) were calculated to evaluate the death characteristics and life loss of the elderly over 60 years old in Huangdu. Results From 2012 to 2019, there were 1 648 deaths among elderly people over 60 years old, and the death rate was 22.56‰. The mortality rate of residents was basically stable over time (P = 0.315). The top five causes of death were tumor, cerebrovascular disease, cardiovascular disease, respiratory diseases, and injury and poisoning, accounting for 81.67% of all deaths. The mortality of tumors and respiratory diseases was higher in males than in females, and the differences were statistically significant (both P0.05). Among the causes of death of residents aged 60 and above, the number of years of life lost caused by all causes of death was 5,512.5 (person-years), the rate of years of life lost was 88.69‰, and the average number of years of life lost was 8.14 (years/person). Conclusion Tumor and cardiovascular and cerebrovascular diseases were the main causes of death in the elderly in Huangdu area. Family doctors should be used to strengthen the prevention and control measures for cancer, cardiovascular and cerebrovascular diseases to improve the quality of life of the elderly in the area.

18.
China Pharmacy ; (12): 1353-1358, 2020.
Article in Chinese | WPRIM | ID: wpr-821801

ABSTRACT

OBJECTIVE:To study the effects of matrine on proliferation and collagen synthesis of rat hepatic stellate cells CFSC-8B activated by acetaldehyde ,and to investigate its possible mechanism. METHODS :CFSC-8B cells cultured in vitro were divided into blank control group ,model group ,positive control group (2.5 μmol/L colchicine)and matrine low ,medium and high concentration groups (30,60,120 μmol/L). Except for blank control group ,other groups were activated with 200 μmol/L acetaldehyde for 24 h;medicine groups were intervened with relevant medicine for 24 h(blank control group and model group were intervened with equal volume blank medium ). Survival rate of cell was detected by CCK- 8 assay. C ells were divided into blank control group ,model group ,positive control group (2.5 μmol/L colchicine),matrine medium and high concentration groups (60,120 μmol/L),then activated and treated with same method. Hydroxyprolin (Hyp)content in cell culture solution was tested by enzyme digestion. The contents of Col- Ⅰ and Col- Ⅲ in cell culture solution were determined by ELISA. mRNA expressionss of α-SMA,TGF-β1,TβR-І,TβR-Ⅱ,Smad3,Smad4 and Smad 7 in cells were detected by RT-PCR. The protein expressions of α-SMA,TGF-β1,TβR-Ⅰ,TβR- Ⅱ,Smad3,Smad4 and Samd 7 in cells were detected by Western blotting. RESULTS :Compared with blank control group ,survival rate of cells in model group was increased significantly (P<0.05);the contents of Hyp ,Col-Ⅰ and Col- Ⅲ in cell culture solution ,mRNA and its protein expressions of α-SMA,TGF-β1,TβR-Ⅰ,TβR-Ⅱ,Smad3,Smad4 in cells were increased significantly in model group (P<0.05),while the mRNA and protein expression of Smad 7 was decreased significantly(P<0.05). Compared with model group ,survival rate of cells ,the contents of Hyp ,Col-Ⅰ and Col- Ⅲ in cell culture solution,the mRNA and protein expressions of α-SMA and Smad 4 were decreased significantly in positive control group and matrine medium and high concentration groups (P<0.05), while the mRNA and protein expression of Smad 7 was WF-0099) increased significantly (P<0.05);the mRNA and proteinexpressions of TGF-β1,TβR-Ⅰ,TβR-Ⅱ and Smad 3 were decreased significantly in positive control group and matrine high concentration group (P<0.05). Compared with matrine medium concentration group ,all above indexes were improved significantly in matrine high concentration group (P<0.05). CONCLUSIONS :Matrine can suppress the proliferation and collagen synthesis of CFSC- 8B cells activated by acetaldehyde ,with a centain concentrlation dependence ,the mechanism of which may be associated with regulating the conduction of TGFβ/Smad signal pathway.

19.
Chinese Journal of Epidemiology ; (12): 506-509, 2020.
Article in Chinese | WPRIM | ID: wpr-811650

ABSTRACT

Objective@#To understand the possible transmission route of a family cluster of COVID-19 in Zhengzhou and the potential infectivity of COVID-19 in incubation period, and provide scientific evidence for the timely control of infectious source and curb the spread of the epidemic.@*Methods@#Epidemiological investigation was conducted for a family cluster of COVID-19 (8 cases) with descriptive epidemiological method, and respiratory tract samples of the cases were collected for the nucleic acid detection of 2019-nCoV by RT-PCR.@*Results@#Two primary cases, which occurred on 31 January and 1 February, 2020, respectively, had a common exposure history in Wuhan. The other six family members had onsets on 30 January, 31 January, 1 February (three cases) and 3 February, 2020.@*Conclusions@#In this family cluster of COVID-19, six family members were infected through common family exposure to the 2 primary cases. Five secondary cases had onsets earlier than or on the same day as the primary cases, indicating that COVID-19 is contagious in incubation period, and the home isolation in the early phase of the epidemic might lead to the risk of family cluster of COVID-19.

20.
Article in Chinese | WPRIM | ID: wpr-733443

ABSTRACT

Objective To investigate the relationship between elderly hypertension patients with frailty syndrome and oral medication compliance. Methods Two hundred elderly patients with hypertensionwho visited the department of cardiology of the First Hospital Affiliated to Dalian Medical University from March 2015 to March 2017were randomly selected. General information was recorded for each patient. The Tilburg Frailty Indicator (TFI), Healthy behavior inventory (HBI) and medication adherence inventory (mmas-8) were assessed for each patient. The population variable and TFI were taken as independent variables, and the mmas-8 score and HBI score were taken as dependent variables to evaluate the linear correlation between each independent variable and each dependent variable. The above independent variables were used as covariates and the results were corrected by disordered multiple Logistic regression analysis. Results The average age of 200 patients was (73.5 ± 7.5) years old, including 103 women (51.5%, 103/200), 97 males (48.5%, 97/200), and the average medical history of hypertension was (12.5 ± 6.1) years. There were 74 patients (37%, 74/200) and 126 patients (63%, 126/200). The mean TFI was 4.3 ± 3.0. The average scores of physical, psychological and social factors were 7.6±3.1, 3.9±2.0, 1.4±0.9, and the average HBI was 3.3±0.3. Among them, the average scores of healthy eating habits, preventive health care, positive mentality and healthy behavior were 3.3±0.8, 3.3±0.4, 3.4± 0.5, and 3.4 ± 0.5, respectively. The mean MMAS-8 was 5.2 ± 2.0, with 144 patients (72%, 144/200) with low compliance and 38 (19%, 38/200) with moderate compliance. Age (Rho:- 0.443), history of hypertension (r:-0.421), TFI score (Rho:-0.483) were negatively correlated with drug adherence (P<0.05). Married/cohabiting patients (tau_b: 0.293), education level (tau_b:0.391), monthly income (tau_b: 0.398) were positively correlated with drug compliance, and the latter two were significantly positively correlated with drug compliance (P<0.01). Monthly income (tau_b:0.371) was significantly positively correlated with HBI, and TFI score (Rho:-0.364) were significantly negatively correlated with HBI, P<0.01. Logistic regression analysis suggested that education degree was a positive determinant of drug compliance (mmas-8) (B=0.81,P=0.01), and monthly income was a positive determinant of health behavior (HBI). Frailty syndrome (TFI) is a negative determinant of poor drug adherence (B=-0.11, P<0.01) and poor health behavior (B=-0.71, P=0.03). Conclusions Frailty syndrome is an important independent factor in predicting drug adherence and adherence to healthy behaviors. High education level improves patient compliance, and good financial status can make patients more active in adherence to healthy behaviors.

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