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1.
Article in Chinese | WPRIM | ID: wpr-931579

ABSTRACT

Objective:To investigate the clinical effects of moxifloxacin combined with biapenem on controlling inflammatory responses and improving immune function in older adult patients with severe pneumonia.Methods:120 older adult patients with severe pneumonia, who received treatment in the Second People's Hospital of Lishui from February 2017 to March 2020, were included in this study. They were randomly assigned to receive either biapenem (control group, n = 55) or moxifloxacin combined with biapenem (observation group, n = 65) for 7 days. Inflammatory response control, immune function improvement, and adverse reactions were assessed in each group after treatment. Results:After treatment, levels of interleukin-6 (IL-6), high-sensitivity C-reactive protein (hs-CRP) and procalcitonin (PCT) in the observation group were (24.51 ± 4.24) ng/L, (12.51 ± 3.15) mg/L and (4.62 ± 1.05) μg/L, respectively, which were significantly lower than those in the control group [(48.74 ± 4.67) ng/L, (26.80 ± 4.24) mg/L, (8.92 ± 1.10) μg/L, t = 29.77, 21.14, 21.87, all P < 0.001). The proportion of CD 3+ and CD 4+ cells and CD 4+ / CD 8+ ratio in the observation group were (63.27 ± 3.72)%, (39.97 ± 2.18)%, 1.79 ± 0.16, respectively, which were significantly higher than those in the control group [(55.58 ± 2.28)%, (35.18 ± 2.62)%, 1.41 ± 0.15, t = 17.08, 10.93, 13.34, all P < 0.001). Total effective rate was significantly higher in the observation group than in the control group [96.92% (63/65) vs. 83.64% (46/55), χ2 = 6.32, P < 0.05]. There was no significant difference in the incidence of adverse reactions between observation and control groups [10.77% (7/65) vs. 9.09% (5/55), χ2 = 0.09, P > 0.05]. Conclusion:Moxifloxacin combined with biapenem based on routine treatment for severe pneumonia in older adult patients can greatly strengthen anti-inflammatory effects, improve immune function, and enhance clinical efficacy without increasing adverse drug reactions.

2.
Protein & Cell ; (12): 639-652, 2021.
Article in English | WPRIM | ID: wpr-888708

ABSTRACT

Rett syndrome (RTT) is a progressive neurodevelopmental disorder, mainly caused by mutations in MeCP2 and currently with no cure. We report here that neurons from R106W MeCP2 RTT human iPSCs as well as human embryonic stem cells after MeCP2 knockdown exhibit consistent and long-lasting impairment in maturation as indicated by impaired action potentials and passive membrane properties as well as reduced soma size and spine density. Moreover, RTT-inherent defects in neuronal maturation could be pan-neuronal and occurred in neurons with both dorsal and ventral forebrain features. Knockdown of MeCP2 led to more severe neuronal deficits as compared to RTT iPSC-derived neurons, which appeared to retain partial function. Strikingly, consistent deficits in nuclear size, dendritic complexity and circuitry-dependent spontaneous postsynaptic currents could only be observed in MeCP2 knockdown neurons but not RTT iPSC-derived neurons. Both neuron-intrinsic and circuitry-dependent deficits of MeCP2-deficient neurons could be fully or partially rescued by re-expression of wild type or T158M MeCP2, strengthening the dosage dependency of MeCP2 on disease phenotypes and also the partial function of the mutant. Our findings thus reveal stable neuronal maturation deficits and unexpectedly, graded sensitivities of neuron-inherent and neural transmission phenotypes towards the extent of MeCP2 deficiency, which is informative for future therapeutic development.

3.
Article in Chinese | WPRIM | ID: wpr-883926

ABSTRACT

Objective:To detect the potential biomarkers in the serum of adolescents with depressive disorder and provide the basis for clinical diagnosis of depression.Methods:A total of 45 adolescents with depression who met the inclusion criteria and 47 healthy controls (HC group) were screened.Patients with depression were treated with sertraline (100 mg/d) for 4 weeks.The patients before sertraline treatment were called DN-MDD group and then were called DT-MDD group after sertraline treatmant.Hamilton Depression Scale (HAMD) was used to evaluate the efficacy of the two groups before and after treatment.The serum was collected for UPLC-MS/MS to determine the changes in profiles of endogenous metabolites.And the software of SPSS 21.0, XCMS and Analyst 3.0 were used to analyze the data.The reliability and feasibility of clinical diagnosis were further analyzed by ROC via GraphPad 8.0.Results:After 4 weeks of sertraline treatment, the patients' HAMD score decreased (before: 19.71±1.72, after: 15.36±1.91) and the effective rate reached 51% ( P<0.01). Multivariate statistical analysis showed that HC group and DN-MDD group and DT-MDD group had significant differences in metabolic profile.Compared with healthy controls, 11 different metabolites were found in the plasma of DN-MDD group including glycerate, adenosine, creatine and so on.After treatment, concentrations of glucose, glutamate, aspartate, sucrose, creatine, pyruvate and adenosine were significant changes in serum of adolescent depression patients.Area under ROC curve of glucose and glutamic acid were >0.9, and the AUC of aspartic acid, sucrose, creatine, pyruvate and adenosine were >0.7, suggesting that these 7 metabolites had different degrees of diagnostic ability. Conclusion:There are different metabolic profiles between adolescents with or without depression, and the antidepressant sertraline is helpful to maintain the level of substance metabolism which can be used as one of the treatment methods for adolescent depression.Our research may support the new strategy for clinical diagnosis and treatment of adolescent depression.

4.
Chinese Journal of Orthopaedics ; (12): 1052-1058, 2021.
Article in Chinese | WPRIM | ID: wpr-910690

ABSTRACT

Objective:To analyze the risk factors of deep venous thrombosis (DVT) in patients with tibial plateau fracture during preoperative period.Methods:From July 2017 to October 2019, a total of 264 patients undergoing surgeries of tibial plateau fractures were enrolled. Duplex ultrasonography (DUS) during hospitalization was used to screen for DVT of the bilateral lower extremities. Patients were divided into DVT group and non-DVT group according to results of DUS. Data on demographics, comorbidities, injury-related data, fracture type, laboratory biomarkers were collected and compared between groups with and without DVT.Results:The incidence of preoperative DVT was 39.0% (103/264) among 264 patients with traumatic tibial plateau fractures, and distal thrombosis predominated in DVT group. There were 103 cases in DVT group. 55 were males and 48 were females. The average age was 54.00±11.12. According to the Schatzker classification of tibial plateau fractures, 7 cases belonged to type I, 37 to type II, 2 to type III, 11 to type IV, 29 to type V, and 17 to type VI. There were 161 cases in non-DVT group. 89 were males and 72 were females. The average age was 48.57±13.25. According to the Schatzker classification of tibial plateau fractures, 23 cases belonged to type I, 76 to type II, 2 to type III, 10 to type IV, 33 to type V, and 17 to type VI. Univariate analysis showed that age ( t=3.451, P=0.001), the type of tibial plateau fracture ( χ2=8.314, P=0.004), D-dimer ( χ2=18.552, P<0.001), APTT ( t=2.869, P=0.004), ALB ( t=2.292, P=0.023) and Hb ( t=1.983, P=0.048) were statistically different than those in non-DVT group. Multivariate analysis showed age ( OR=1.033, 95% CI: 1.009, 1.058; P=0.007), the type of tibial plateau fracture ( OR=1.829, 95% CI: 1.014, 3.299; P=0.045) and D-dimer ( OR=1.914, 95% CI: 1.057, 3.464; P=0.032) were independent risk factors. Conclusion:The incidence of DVT in patients with tibial plateau fractures during preoperative period is high, and distal thrombosis is the main part of venous thrombosis of lower extremity. The type of tibial plateau fracture, age and the level of D-dimer are independent risk factors of preoperative DVT in patients with tibial plateau fractures.

5.
Article in Chinese | WPRIM | ID: wpr-866370

ABSTRACT

Objective:To explore the multivariate logistic regression analysis and imaging characteristics of chronic obstructive pulmonary disease (COPD) with invasive pulmonary aspergillosis.Methods:From February 2017 to February 2019, 73 patients with COPD complicated with invasive pulmonary aspergillosis admitted to the Hangzhou Dajiangdong Hospital were selected as observation group, and 81 COPD patients without invasive pulmonary aspergillosis admitted to the Hangzhou Dajiangdong Hospital from February 2017 to February 2019 were selected as control group.Multivariate logistic regression analysis was used to analyze the independent risk factors of COPD with invasive pulmonary aspergillosis, and to observe the imaging characteristics of COPD with invasive pulmonary aspergillosis.Results:Univariate analysis showed that there were no statistically significant differences in sex, age, course of disease, combined with underlying diseases and smoking history between the two groups (all P>0.05); there were statistically significant differences in long-term hormone use, hypoproteinemia, long-term antibiotics use and invasive operation between the two groups (χ 2=32.372, 16.593, 36.671, 21.566, all P<0.05). The results of logistic regression analysis showed that long-term use of hormones, hypoproteinemia, antibiotics and invasive manipulation were independent risk factors for COPD complicated with invasive pulmonary aspergillosis ( OR=1.685, 95% CI: 1.208-2.142, OR=2.546, 95% CI: 1.682-3.981, OR=2.984, 95% CI: 1.982-4.653, OR=2.109, 95% CI: 1.462-3.241, all P<0.05). The main clinical manifestations of COPD with invasive pulmonary aspergillosis were cough, sputum, chest pain and dyspnea.The main imaging manifestation of COPD with invasive pulmonary aspergillosis was non-specific infiltration, accounting for 50.68%(37/73). Conclusion:COPD complicated with invasive pulmonary aspergillosis is affected by many factors.The main clinical manifestations are cough, sputum, chest pain and dyspnea, and the main imaging manifestation is non-specific infiltration shadow.

6.
Article in Chinese | WPRIM | ID: wpr-733453

ABSTRACT

Objective To construct a"Field and Disaster Nursing"course which is suitable for the military and local higher nursing education, on-the-job and post training education to organic integrate the modern disaster nursing teaching and military field nursing teaching. Methods Based on the methods of qualitative and quantitative research, such as literature research, interviews and observational method, a preliminary plan of curriculum design was established, and an expert consultation table was designed.The Delphi expert consultation method was used to conduct two rounds of consultation with 30 related experts on disaster relief.Quantitative data were obtained and statistical data were analyzed. Results The final screening results of the curriculum content applicability index are 4 first grade, and 23 for the two level.A set of"Field and Disaster Nursing"curriculum had been set up that is suitable for education and training needs of the military and local fusion. Conclusion The construction of this course provides a training thought for exploring disaster nursing education suitable for China's national conditions, and has reference and guiding significance for further implementation of military and civilian integration and multi participatory disaster nursing teaching practice.

7.
Chongqing Medicine ; (36): 1436-1439, 2018.
Article in Chinese | WPRIM | ID: wpr-691966

ABSTRACT

Objective To investigate feasibility of CM-Dil labeling mesenchymal stem cells in vitro.Methods We separate,culture and identify rat bone marrow mesenchymal stem cells,after labeling MSCs with CM-Dil concentration respectively in 1,2,4μg/mL,observe label rate of MSCs after 15 min,24 h,48 h,72 h,5 passage and 8 passage,then detect cell cycle with FCM and cell proliferation capability with CCK8.Results MSC cultured with the whole bone marrow adherent method were strongly positive for CD44,CD29 and negative for CD34,CD45.The label rates of MSC 15 min after labeled with CM-Dil 1,2,4 μg/mL were respectively (31.60±1.25)%,(88.73±1.79)%,(96.89±1.31)%,and there has statistically difference (F=1 757.21,P=0.000);the growth curve of the 4 groups was similar,and the percentages of G1 phase,S phase and G2/M phase between the 4 groups have no statistically difference (P>0.05).Conclusion CM-Dil in concentration 4 μg/mL has high label rate and low cytotoxicity,therefore could be a efficient and stable method of labeling MSCs.

8.
Article in Chinese | WPRIM | ID: wpr-610401

ABSTRACT

Objective The aim of this study was to explore the inhibitory effect of alcohol extracts of Narcissus bulb on melanogenesis in zebrafish embryos.Methods Zebrafish embryos were exposed to different concentrations of alcohol extracts of Narcissus (0, 50, 100, 200, and 500 μg/mL), and then the formation of melanin was observed.Furthermore, we measured the enzyme activity of tyrosinase (TYR), which plays a pivotal role in melanogenesis.Meanwhile, the spatial and temporal pattern of melanin-specific marker genes were detected.Arbutin (Ar) was used as a positive control in all these experiments.Results The treatment of alcohol extracts of Narcissus bulb inhibited melanogenesis in the zebrafish embryos in a dose-dependent manner.Furthermore, the mRNA expression levels of melanin-related genes such as tyrosinase (TYR), silver (SILV) and Mitfa were significantly reduced after treatment with different concentrations of Narcissus extracts by in-situ and semi-quantitative PCR.Finally, the enzyme activity of tyrosinase was also gradually decreased with increasing concentrations of the alcohol extracts.Conclusions Narcissus alcohol extracts can effectively inhibit the production of melanin in zebrafish embryos.This study provides potential evidence and approaches for the screening of natural whitening compounds using zebrafish models.

9.
Chinese Medical Ethics ; (6): 466-470, 2017.
Article in Chinese | WPRIM | ID: wpr-609555

ABSTRACT

Objective:To investigate the current status of,analyze its influencing factors and explore educational countermeasures,thus to provide evidence for establishing prevention model of medical students' soft addiction in medical colleges.Method:Using the self-designed questionnaire,a questionnaire survey related tosoft addiction was conduct among 780 medical students.The results were analyzed statistically.Results:At present,the proportion of soft addiction in medical students is relatively large.These students will affect their study and work because of the addiction to certain behaviors and have a negative impact on personal growth and self-development.Conclusion:University should pay attention to the prevention and treatment of medical students' soft addiction behavior.It should change the social concept of medical students,enhance interpersonal ability;improve their self-cultivation,stimulate the self-education;focus on time management,improve efficiency,overcome procrastination.

10.
Article in Chinese | WPRIM | ID: wpr-507238

ABSTRACT

Objective To discuss the diagnostic value of HIT-antibodies in suspected HIT patients with heart diseases.Methods A single center study.We collected 242 blood samples of suspected HIT patients whose platelet count decreased after heparin application during July 1 st ,2012 to June 30th ,2016 in Wuhan Asia Heart Hospital and detected the concentration of HIT antibodies , meanwhile the 4T′s score were calculated.Among the study objects , there are 206 patients received cardiac surgery , 28 received cardiac interventional therapy and 8 received drug therapy.And we divided them into HIT group (44, median age 57.5, 23 females ) and non-HIT group ( 198, median age 63.5, 87 females ) according to clinical diagnosis.Quantitative data was analyzed by independent t-test or Mann-Whitney U test.Qualitative data was analyzed by Fisher′s exact test.We drew ROC curve according to the statistical analysis to determine the optimal threshold value of antibodies in diagnosis of HIT andsensitivity , specificity, negative likelihood ratio, positive likelihood ratio of the HIT antibody detection .Therefore, we can assess the value of HIT antibody detection in HIT clinical diagnosis and treatment .Moreover, we used the optimal threshold value of antibodies to testify the suspected HIT patients .Results The HIT antibody concentration of HIT group (44) and non-HIT group ( 198 ) are 3.2 ( 95% CI:1.8 -5.5 ) U/ml and 0.4 ( 95% CI:0.3 -0.4 ) U/ml, respectively.The concentration of HIT group is much higher than the non-HIT group(P<0.000).When the cut-off value of HIT-Ab is set at 0.9 U/ml, sensitivity and specificity are 93.2%and 91.9%, respectively. And negative likelihood ratio and positive likelihood ratio are 0.07 and 11.53, respectively.When the cut-off value of HIT-Ab is set at 0.6 U/ml, sensitivity and specificity are 100.0%and 73.7%.HIT-Ab and 4T′s score of ROC-AUC are 0.971 and 0.745, respectively.The diagnosis value of HIT-Ab in HIT is significantly higher than the 4T′s score ( P<0.000).Conclusions HIT antibody detection is a simple and effective auxiliary diagnostic method in HIT exclusion .And HIT antibody detection is more optimal than the 4T′s score in HIT diagnosis and treatment .

11.
Article in Chinese | WPRIM | ID: wpr-662547

ABSTRACT

Objective To analyze drug resistance and epidemic features of Acinetobacter baumannii in two hospitals of Rushan city .Methods Acinetobacter baumannii strains were collected from Rushan People′s Hospital and Chinese Medicine Hospital of Rushan in 2015 .Antimicrobial susceptibility test was carried out by Kirby-Bauer(K-B) method .Homology analysis were conducted by pulsed-field gel electrophoresis(PFGE) in 50 Acinetobacter baumannii strains .Results There were 158 Acinetobacter baumannii strains were isolated ,108 strains were from Rushan People′s Hospital ,50 strains were from Chinese Medicine Hospital of Rushan . 59 .9% strains was from sputum specimens .The most strains were from Respiratory department (39 .8% ) and ICU (25 .3% ) .All strains were resistant to aztreonam ,ampicillin and cefoxitin ,but sensitive to polymyxin B and tigecycline .50 strains could be divided into five types(A -E) .A typewas the predominant type ,including A1 ,A2 ,A3 three subtypes .Conclusion The resistance of Acine-tobacter baumannii was serious in the two hospitals in Rushan city .It mainly cause respiratory infections ,and could spread among different departments .The most strains were from Respiratory Department and ICU .

12.
Article in Chinese | WPRIM | ID: wpr-660263

ABSTRACT

Objective To analyze drug resistance and epidemic features of Acinetobacter baumannii in two hospitals of Rushan city .Methods Acinetobacter baumannii strains were collected from Rushan People′s Hospital and Chinese Medicine Hospital of Rushan in 2015 .Antimicrobial susceptibility test was carried out by Kirby-Bauer(K-B) method .Homology analysis were conducted by pulsed-field gel electrophoresis(PFGE) in 50 Acinetobacter baumannii strains .Results There were 158 Acinetobacter baumannii strains were isolated ,108 strains were from Rushan People′s Hospital ,50 strains were from Chinese Medicine Hospital of Rushan . 59 .9% strains was from sputum specimens .The most strains were from Respiratory department (39 .8% ) and ICU (25 .3% ) .All strains were resistant to aztreonam ,ampicillin and cefoxitin ,but sensitive to polymyxin B and tigecycline .50 strains could be divided into five types(A -E) .A typewas the predominant type ,including A1 ,A2 ,A3 three subtypes .Conclusion The resistance of Acine-tobacter baumannii was serious in the two hospitals in Rushan city .It mainly cause respiratory infections ,and could spread among different departments .The most strains were from Respiratory Department and ICU .

13.
Chinese Journal of Neonatology ; (6): 115-118, 2017.
Article in Chinese | WPRIM | ID: wpr-514303

ABSTRACT

Objective To study the characteristics of neonatal sepsis caused by gram positive (G +) bacteria,gram negative (G+) bacteria and fungi.Method Clinical data of 202 neonates with sepsis hospitalized from Jan.2012 to May.2015 were studied.According to the different pathogens,202 neonates were divided into gram positive bacteria group,gram negative bacteria group and fungi group.The general information,clinical manifestation,laboratory examination and treatment outcome of the three groups were analysed with Chi square analysis,LSD,Fisher exact probability tests.Result A total of 202 cases of neonatal sepsis were recruited.The detection rate of gram positive bacteria,gram negative bacteria and fungi was 35.2% (71 cases),56.4% (114 cases) and 8.4% (17 cases),respectively.Comparing with gram negative group and fungi group,gram positive group had older gestational age (36.0 ± 3.8 w,compared with gram negative 33.0 ± 3.9 w,fungi group 31.2 ± 3.2 w,P < 0.05),larger birth weight (2 620 ± 925 g compared with gram negative group 1 999 ± 849 g,fungi group 1 595 ± 666 g,P < 0.05),lower nosocomial infection rate (29.6% compared with gram negative group 70.2%,fungi group 94.1%,P <0.05),lower rate of shock,blood glucose disturbance and thrombocytopenia (P < 0.05).There was no statistics difference between the gram negative group and fungi group.Comparing with the other two groups,fungi group was older [20.0 (11.5,39.5) d compared with gram positive group 7.0 (2.0,17.0) d,and gram negative 10.0 (6.0,18.2) d,P < 0.05].The rate of deep venous catheterization in fungi group was higher than that in gram positive group and gram negative group (88.2% compared with gram positive group 25.4%,gram negative group 40.4%,P <0.05).The treatment course of fungi group was longer than that of the gram positive group and gram negative group [22.0 (12.0,37.5) d compared with the gram positive group 14.0 (10.0,17.0) d,gram negative group 14.0 (11.0,18.0) d,P <0.05].The incidence of apnea in the gram negative group was higher than that in gram positive group and fungi group (P < 0.05).The rate of leukocytosis,leukocytopenia and elevated CRP were higher in gram negative and gram positive group (P < 0.05).Conclusion The clinical manifestations and laboratory examinations in neonatal sepsis caused by different pathogens were different,which can help to early identification of different pathogenic infections.However,there is no specific indicators to differentiate neonatal sepsis caused by different pathogens.Early identification of the pathogen needs clinical acumen.

14.
Modern Clinical Nursing ; (6): 28-31, 2017.
Article in Chinese | WPRIM | ID: wpr-616955

ABSTRACT

Objective To observe the effect of red light facial synthesis combined with comprehensive facial therapy on corticosteroid dependent dermatitis (hormone dependent dermatitis) and summarize the nursing strategies. Methods About 160 patients with hormone dependent dermatitis hospitalized in our hospital from June 2015 to March 2016, were equally divided into control group and study group:the control group was treated with conventional comprehensive therapy, while the study group with 30 min red light irradiation together with the nursing measures in the control group. The total symptom scores of the two groups were evaluated before treatment and 4 weeks after treatment. Results Before treatment, the total symptom scores of the control group were no significant difference between them (P>0.05). After treatment,the treatment effect of the study group was significantly better than that of the control group and the scores of ISS SSRI were lower than those of the control group . Conclusion The red light radiation combined with comprehensive facial therapy is effective in the treatment of corticosteroid dependent dermatitis and related nursing should be done well to promote patients relovery.

15.
Chinese Journal of Epidemiology ; (12): 142-146, 2017.
Article in Chinese | WPRIM | ID: wpr-737617

ABSTRACT

Objectives To understand the medication being used among patients with chronic obstructive pulmonary diseases (COPD) in Chinese communities of large cities.Methods A cross-sectional survey was carried out in Beijing,Shanghai,Chengdu and Guangzhou with the total number as 678 COPD cases who were continuously recruited.Subjects were face-to-face interviewed using a structured questionnaire and with medical records checked at the same time.All data were double entered into a database under EpiData 3.1,and analyzed by SAS 9.2.Results One quarter of all the subjects did not receive any clinical treatment.The top three drugs for COPD were expectorant (50.74%),phosphodiesterase inhibitors (49.56%),and leukotriene (49.12%).Totally,there were 36 different types combination of drugs used for COPD and the proportions of drugs being used as one,two,and three or more at the same time were 29.5%(200 cases),39.7%(269 cases) and 30.8%(209 cases) respectively.In addition 36.6% (248 cases) of them used oxygen.There were statistical correlations between drug-use patterns and the severity of COPD.Conclusion The patterns of medication varied over COPD cases in communities from Chinese large cities and appeared differently under the recommendations in the clinical guidelines set for COPD.Guidance and surveillance programs on drugs use for COPD should be improved on patients with COPD in the Chinese communities.

16.
Chinese Journal of Epidemiology ; (12): 142-146, 2017.
Article in Chinese | WPRIM | ID: wpr-736149

ABSTRACT

Objectives To understand the medication being used among patients with chronic obstructive pulmonary diseases (COPD) in Chinese communities of large cities.Methods A cross-sectional survey was carried out in Beijing,Shanghai,Chengdu and Guangzhou with the total number as 678 COPD cases who were continuously recruited.Subjects were face-to-face interviewed using a structured questionnaire and with medical records checked at the same time.All data were double entered into a database under EpiData 3.1,and analyzed by SAS 9.2.Results One quarter of all the subjects did not receive any clinical treatment.The top three drugs for COPD were expectorant (50.74%),phosphodiesterase inhibitors (49.56%),and leukotriene (49.12%).Totally,there were 36 different types combination of drugs used for COPD and the proportions of drugs being used as one,two,and three or more at the same time were 29.5%(200 cases),39.7%(269 cases) and 30.8%(209 cases) respectively.In addition 36.6% (248 cases) of them used oxygen.There were statistical correlations between drug-use patterns and the severity of COPD.Conclusion The patterns of medication varied over COPD cases in communities from Chinese large cities and appeared differently under the recommendations in the clinical guidelines set for COPD.Guidance and surveillance programs on drugs use for COPD should be improved on patients with COPD in the Chinese communities.

17.
Article in Chinese | WPRIM | ID: wpr-492738

ABSTRACT

Achieving the millennium development goals and world peace and development are closely linked objectives, and WHO having been made great achievements and progress in the health sector through its related ob -jectives.All health-related millennium development goals such as maternal and child health , HIV/AIDS prevention and control , malaria and tuberculosis , safe drinking water and sanitation , and foreign medical assistance had been basically reached in China .This success was mainly due to the government attention and commitment , legal protec-tion, health information technology-informatization, effective projects and measures , but there are still differences in health status between regions and population groups , and increasing needs of health services quality improvement and chronic diseases control and prevention should be paid great attention in the future .

18.
Article in Chinese | WPRIM | ID: wpr-490452

ABSTRACT

Objective To investigate the influence of Ulinastatin (UTI) on the hyper-permeability of vascular endothelial cells induced by tumor necrosis factor alpha (TNF-α).Methods Inflammation model was induced by TNF-α in human umbilical vein endothelial cell line (EA.hy926).The experiment was designed into 4 groups:normal group,TNF-α group,UTI group and TNF-α with UTI (U + T) group.Methyl thiazolyl tetrazolium (MTT) method and epithelial voltameter (EVOM) method were used to measure cell viability [absorbance (A) value] and transepithelial electrical resistance (TER) of EA.hy926 cells respectively.The expression of VE-cadherin was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry.Results Compared with normal group,the TER of EA.hy926 cells induced by TNF-α was significantly decreased (67.200 ± 8.937 vs.33.600 ± 8.771,P =0.010).The permeability in EA.hy926 cells increased obviously.The hyper-permeability of EA.hy926 cells induced by TNF-α could be alleviated by UTI at the concentrations of 1-100 U/mL in a dose-dependent manner (40.133 ±7.484 vs.33.600 ±8.771,P=0.382;49.232 ± 3.162 vs.33.600 ± 8.771,P =0.044;63.700 ± 8.515 vs.33.600 ± 8.771,P =0.013).The expression of VE-cadherin mRNA reduced significantly in the TNF-α group (1.089 ±0.018 vs.0.835±0.021,P =0.000) compared with normal group.This effect of TNF-α could be attenuated by UTI.When EA.hy926 cells exposed to UTI at 10 U/mL and 100 U/mL,a significant increase of the expression of VE-cadherin mRNA was observed (0.976 ±0.014 vs.0.835 ±0.021,P =0.001;1.115 ±0.015 vs.0.835 ± 0.021,P =0.000).And the inhibition of UTI manifested a dose-dependent manner (1-100 U/mL).The results of the immunocytochemistry showed that the expression of VE-cadherin in TNF-o group was decreased significantly (0.061 ± 0.013 vs.0.093 ± 0.014,P =0.049) compared with normal group.And the low-expression of VE-cadherin could be alleviated by UTI (0.032 ± 0.004 vs.0.061 ± 0.013,P =0.016).Conclusion The high permeability of EA.hy926 cells induced by TNF-α could be inhibited by UTI at the concentrations of 1-100 U/mL in a dose-dependent manner.

19.
Protein & Cell ; (12): 175-186, 2016.
Article in English | WPRIM | ID: wpr-757145

ABSTRACT

The mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion arise. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.


Subject(s)
Antigens, Differentiation , Electrophysiological Phenomena , Physiology , Gene Expression Regulation , Physiology , Genome-Wide Association Study , Human Embryonic Stem Cells , Cell Biology , Metabolism , Humans , Induced Pluripotent Stem Cells , Cell Biology , Metabolism , Multigene Family , Physiology , Neurons , Cell Biology , Metabolism , Transcriptome , Physiology
20.
Article in Chinese | WPRIM | ID: wpr-498567

ABSTRACT

Objects To investigate the correlation of the single nucleotide polymorphism ( SNP) of clopidogrel related gene CYP2C19, ABCB1, PON1 to the occurrence of clopidogrel resistance ( CR) and TEG among patients after percutaneous coronary intervention.Methods A total of 299 patients after PCI were enrolled from April 2015 to December 2015.It genotyped the CYP2C19(rs4244285,rs4986893)ABCB1 ( rs1045642 ) and PON1 ( rs662 ) gene, measured clopidogrel response by TEG.Accordingly, all the enrolled 299 patients were then divided into CR group (n=17) +non-CR (NCR) group (n=282) or CLR group (n=54) +non-CR (NCR) group (n=245) by TEG(%).All the patients were divided into EM、IM and PM group by CYP2C19 genotype.The age of patient in CR (71.1 ±11.1) years old is higher than NCR (65.02 ±10.51) years old (t=2.559, P0.05).There was no significant difference between CR(χ2 =0.175,P=0.916) CLR(χ2 =1.589,P=0.452)and the level of TEG(ADP) (Z=-0.030,P=0.976) in PON1(rs662) polymorphism.There was no significant difference between CR(χ2 =1.722,P=0.423) CLR(χ2 =0.176,P=0.916) and the level of TEG(ADP) (Z=-0.331,P=0.741) in ABCB1(rs1045642) polymorphism.Conclusions CYP2C19 PM is associated with decreased of TEG(ADP).It is considered that no correlation exists between ABCB1(rs1045642) and PON1(rs662) polymorphism and clopidogrel resistance in patients with coronary heart diseases.The loss of function of ABCB1 ( rs1045642 ) and PON1 ( rs662 ) is not associated with decreased of TEG(ADP) in CYP2C19 PM patients.

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