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1.
Article in Chinese | WPRIM | ID: wpr-912312

ABSTRACT

Objective:To investigate the risk and efficacy of the decortication in treatment of drug-resistant tuberculous empyema.Methods:A retrospective analysis was conducted on the 146 patients with tuberculous empyema, who underwent decortication in Wuhan Pulmonary Hospital from March 2016 to November 2018, according to the drug-susceptibility testing of the pleural effusion or tissue specimens, the patients had been divided into drug-resistant group(29 cases) and control group(117 cases), compared the clinical datas such as operation time, intraoperative blood loss, postoperative drainage duration, pulmonary reexpansion duration, postoperative complications and curative ratio between both groups, and the factors that influence the risk and efficacy of surgery had been analyzed.Results:The operative time, intraoperative blood loss, postoperative drainage duration and postoperative complications in the drug-resistant group were larger than those in the control group, and the differences were statistically significant ( P<0.05). There was no significant difference in pulmonary reexpansion duration and curative ratio in the two groups( P>0.05). Conclusion:Decortication is safety, effective and feasible in the treatment of drug-resistant tuberculous empyema, although the operation is difficult and risky.

2.
Article in Chinese | WPRIM | ID: wpr-911761

ABSTRACT

Sudden cardiac death (SCD) is one of the most common causes of death for patients with cardiovascular diseases. General practitioners are the providers of primary medical and health service, it its worthwhile to discuss their role positioning in the prevention and control system of SCD. In this study, SWOT analysis was used to explore the strength, weakness, opportunity and threats of the involvement of general practitioners in SCD prevention and control system, to clarify the roles of general practitioners and to provide suggestions for the improvement and development of SCD prevention and control system in China.

3.
Article in Chinese | WPRIM | ID: wpr-911715

ABSTRACT

Objective:To survey on the knowledge, attitude and prescribing of benzodiazepines (BZDs) among general practitioners.Methods:A questionnaire survey was conducted in November 2018 among 396 clinicians who participated in the general practice training in Henan Province, including 131 general practitioners (GP group) and 265 non-GPs (non-GP group). The questionnaire included basic information of clinicians, BZDs prescription and training, subjective attitude and knowledge of BZDs.Results:The results of 396 valid questionnaires showed that 55 (13.9%) clinicians had participated in BZDs related training, and 331 (83.6%) clinicians had prescribed BZDs. There was no significant difference in the duration of BZDs continuous use between the GP group and the non-GP group (χ 2= 8.37, P>0.05). The overall score of subjective attitude towards BZDs was low [4(2,6) - 5(3,9)], and the score of BZDs training needs was high [10(8,10)]. Compared to non-GP group, the GP group had lower confidence in BZDs indication [4(2,6) vs. 5(3,6), Z=-2.66, P<0.05 ], the lower ability of judging BZDs addiction [5(2,6) vs. 5(3,7), Z=-2.52, P<0.05], and less understanding of BZDs use guidelines [4(1,5) vs. 4(3,6), Z=-3.05, P<0.05]. In terms of prescription tendency, the GP group was less inclined to prescribe BZDs according to experience [5(2,7) vs. 6(4,8), Z=-3.50, P<0.05]. In the knowledge of BZDs, the total score of GP group was lower than that of non-GP group, the [11(8,12) vs. 11(9,13), Z=-2.06, P<0.05]. The proportion of GPs with drug addiction recognition[66.4%(87/131) vs.77.7%(206/265),χ 2=5.84] and pharmacology knowledge [59.5%(78/131) vs.72.1%(191/265),χ 2=6.32] were all lower than that of non-GPs( P<0.05). Conclusions:General practitioners are lack of knowledge about BZDs and confidence in prescribing BZDs. It is necessary to carry out training on BZDs, especially for addiction and drug effects.

4.
Article in Chinese | WPRIM | ID: wpr-882643

ABSTRACT

Objective:To investigate the effects of different lipid-lowering regimens on blood lipids, endothelial function and safety in patients with unstable angina.Methods:Patients who admitted to Henan Provincial People's Hospital for unstable angina from September 2018 to May 2019 were randomly (random number) divided into the conventional treatment group, intensive statin group and intensive lipid-lowering group. Follow-up was performed at 1, 3, and 6 months after treatment according to the predetermined lipid-lowering regimen. Assessments included lipid profile, liver function, muscle enzymes, hypersensitive C-reactive protein (hs-CRP), endothelial function (reactive hyperemia index, RHI), ischemic events, myalgia, and discontinuation. The differences of the follow-up indicators among the three groups were analyzed.Results:A total of 375 patients were enrolled and randomly divided into three groups, 125 patients in each group. There were no significant differences in demographic data and medication among the three groups. At the 1st month, the low density lipoprotein cholesterin (LDL-C) compliance rate of the intensive statin group was significantly higher than those in the conventional treatment group ( χ2=3.939, P=0.047) and the intensive lipid-lowering group ( χ2=4.63, P=0.031). At the 3rd month, the reductions of LDL-C in the intensive statin group and the intensive lipid-lowering group were significantly better than that in the conventional treatment group( P<0.01). At the 6th month, the reduction rate of LDL-C in the intensive lipid-lowering group was higher than that in the intensive statin group ( q=4.332, P<0.01). At the 1st month, the improvement of hs-CRP and RHI in the intensive statin group was significantly better than that in the conventional treatment group( q=4.133, P<0.05). From the 3rd month of treatment, the incidence of cardiovascular events in the intensive statin group and the intensive lipid-lowering group showed a tendency to decrease compared with the conventional treatment group, but no statistically significant difference was found. At the 6th months of treatment, the withdrawal rates were significantly higher in the intensive statin group and the intensive lipid-lowering group than that in the conventional treatment group (χ 2=4.488, P=0.03 and χ2=5.039, P=0.02). There were no significant differences in the ratio of liver enzyme and muscle enzyme elevation and the incidence of myalgia among the three groups (all P>0.05). Conclusions:Intensive statin therapy can make LDL-C reach the standard in patients with unstable angina pectoris as soon as possible, significantly improve inflammation indicators and endothelial function, and has good safety.

5.
Protein & Cell ; (12): 7-28, 2021.
Article in English | WPRIM | ID: wpr-880895

ABSTRACT

Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to the formation of a totipotent embryo. During the development of the pre-implantation embryo, precise reprogramming progress is a prerequisite for avoiding developmental defects or embryonic lethality, but the underlying molecular mechanisms remain elusive. For the past few years, unprecedented breakthroughs have been made in mapping the regulatory network of dynamic epigenomes during mammalian early embryo development, taking advantage of multiple advances and innovations in low-input genome-wide chromatin analysis technologies. The aim of this review is to highlight the most recent progress in understanding the mechanisms of epigenetic remodeling during early embryogenesis in mammals, including DNA methylation, histone modifications, chromatin accessibility and 3D chromatin organization.


Subject(s)
Animals , Chromatin Assembly and Disassembly , DNA Methylation , DNA Transposable Elements , Embryo, Mammalian , Embryonic Development/genetics , Epigenesis, Genetic , Epigenome , Female , Fertilization/physiology , Gene Expression Regulation, Developmental , Histone Code , Histones/metabolism , Male , Mice , Oocytes/metabolism , Spermatozoa/metabolism
6.
Article in Chinese | WPRIM | ID: wpr-871379

ABSTRACT

Objective:To evaluate the efficacy of hemostatic powder on preventing delayed bleeding after endoscopic submucosal dissection (ESD).Methods:Patients who received ESD in the First Affiliated Hospital of Soochow University and Yulin No.2 Hospital from June 2017 to August 2018 were enrolled with informed consents, and randomly divided into the study group and the control group. Hemostatic powder was applied on post-ESD ulcer after routine hemostasis method in the study group, and the control group was given routine hemostasis method only. The time and dosage of hemostatic powder spraying and its adverse events were observed in the study group. The operation time, rate of delayed bleeding (within 30 days after operation) and early delayed bleeding (within 48 hours after operation), and postoperative hospital stay were compared between the two groups.Results:A total of 196 patients were enrolled including 97 in the study group and 99 in the control group. The baseline data were comparable between the two groups (all P>0.05). In the study group, the time to spray powder was 68.78±19.75 s, dosage was 2.51±0.93 g. Powder delivery catheter was blocked in one case (1.03%, 1/97). No adverse event was reported during 30 days of follow-up. The operation time was not statistically different in the study group and the control group (61.92±11.71 min VS 59.76±11.01 min, t=1.330, P=0.185). The delayed bleeding rate of the study group was significantly lower than that of the control group [1.03% (1/97) VS 8.08% (8/99), P=0.035]. There was no case of early delayed bleeding occurred in the study group, while 6 cases (6.06%, 6/99) in the control group ( P=0.029). The postoperative hospital stay was not statistically different between the study group and the control group (4.57±0.85 d VS 4.86±1.37 d, t=1.778, P=0.077). Conclusion:Although capacity of hemostatic system remains to be improved, hemostatic powder is an effective, safe and simple method to reduce delayed bleeding rate after ESD, especially on early delayed bleeding.

7.
Article in Chinese | WPRIM | ID: wpr-870646

ABSTRACT

Objective:To survey the status general practice residency training and career choice of trainees in Henan Province.Methods:From December 2017 to February 2018, 332 trainees from the first general practice residency training program (2014-2017) were enrolled in the study. The self- administered questionnaire included the demographic information and the current career status. The logistic regression model was used to analyze the influencing factors of the career choice when their completed the training.Results:Of 332 participants, there were 167 participants designated by the institutions and the others were directly enrolled by the program. The designated participants were from the 56 medical institutions in Henan. The percentage of participants from the tertiary hospitals was 75.5% (126/167), which was much higher than that from the secondary hospitals (24.5%,41/167). There were 24 participants who did not have a work six months after graduation. Of the 308 employed participants, there were only 111 (36.0%, 111/308) working in the department of general practice, and 197 (64.0%, 197/308) working in other departments. The participants designated by the institutions and with the post-graduate degrees preferred to choose other specialties, instead of the general practice. The reasons were that there was no department of general practice in their institutions or the participants had been designated to the other departments (57.9%, 114/197).Conclusion:The survey suggests that the general practice residency training program should enroll trainees mainly from the medical graduates in order to improve the effectiveness of general practitioner residency training and the allocation of health resources in Henan Province.

8.
Article in Chinese | WPRIM | ID: wpr-870149

ABSTRACT

Objective:To study the clinical characteristics and classification of gastric neuroendocrine neoplasm(NEN) and prognostic factors of mixed adenoneuroendocrine carcinoma (MANEC) and gastric neuroendocrine carcinoma(NEC).Methods:A total of 148 gastric NENs were divided into type Ⅰ, type Ⅱ and type Ⅲ based on the classification of European Neuroendocrine Tumor Society (ENETS). Kaplan-Meier test and Cox regression model were used in univariate and multivariate survival analysis in 108 cases with pathological G3 gastric NEN.Results:In this study, the percentages of type Ⅰ, type Ⅱ and type Ⅲ were 25.0%(37), 3.4%(5) and 71.6%(106) respectively. Among type Ⅰ patients, 28(75.7%) lesions were located in gastric fundus or body, 29(78.4%) had bumps. Lymph node involvement was found in 4 (10.8%) patients. Twenty-six (70.3%) patients received endoscopic treatment and 11 (29.7%) with surgery. All 5 type Ⅱ patients presented lesions in gastric fundus or body, including 4 with ulcers, who were all treated by endoscope. Three type Ⅱ patients had gastrinoma, and 2 combined with multiple endocrine neoplasmⅠ. In type Ⅲ patients, 56(52.8%) showed ulcerative lesions. The majority of patients (102, 96.2%) had a single lesion, 94(88.7%) with lymph node or other organ metastasis. In this study, no deaths were reported in gastric NEN with a pathological grade of G1 or G2. The mortality rate was 38.9%(42/108) in patients with G3 NEN. Survival analysis suggested that age, metastasis of tumor were associated with poor prognosis ( P=0.041, 0.025). Conclusions:Patients with gastric NEN have heterogenous clinical presentations according to gender, age, endoscopic features, infiltration and metastasis, and pathological grade. Aging and metastasis are negative prognostic factors of G3 gastric NEN.

9.
Chinese Journal of Geriatrics ; (12): 509-513, 2020.
Article in Chinese | WPRIM | ID: wpr-869415

ABSTRACT

Objective:To explore the relationship between plasma homocysteine level and renal dysfunction in elderly patients with coronary heart disease(CHD).Methods:Eight hundreds and fifty-eight elderly patients with CHD were enrolled at cardiology department of our hospital from January 2015 to July 2017.The patients were divided into the renal insufficiency(RI, defined as an estimated glomerular filtration rate(eGFR)<60 ml/min)group and the non-kidney disease(NKD)group.Using the random number table, the RI group was randomly sub-grouped into the intervention group and the placebo group.The intervention group was treated with folic acid(10 mg/d), vitamin B6(10 mg/d)and vitamin B12(1 mg/d), while the placebo group was given oral placebo.Serum homocysteine(Hcy), creatinine(Cr), blood urea nitrogen(BUN), total cholesterol(TC), triglyceride(TG)and hemoglobin(Hb)were measured by enzyme-linked immunosorbent assay.Pearson correlation was used to analyze the correlations of Hcy with Cr, BUN and eGFR between the RI and NKD groups.Plasma Hcy, Cr, BUN and Hb levels before, and 6, 12 months after treatment were determined and compared between the two subgroups.Staging of renal function was analyzed and clinical effect was evaluated 12 months after treatment in the two subgroups.Results:Serum levels of Hcy, Cr and BUN were higher( t=3.174, 4.857 and 2.644, all P=0.000)and the level of eGFR was lower( t=-2.867, P=0.000)in the RI group than in the NKD group.Plasma Hcy level was positively correlated with plasma levels of Cr and BUN( r=0.308 and 1.214, all P=0.000)and negatively correlated with the eGFR level( r=-0.148, P=0.003). There was no significant difference in plasma Hcy, Hb, Cr and BUN levels between the intervention and placebo group before treatment( P>0.05). At each monitoring time point, serum levels of Hcy, Cr and BUN were decreased continuously in the intervention group( F=314.527, 234.861 and 176.332, P=0.012, 0.000 and 0.006), while plasma Hcy level had no significant change and plasma levels of Cr and BUN were increased continuously in the placebo group( F=196.427, 223.753 and 314.552, P=0.216, 0.000 and 0.002). After 12 months of treatment, plasma levels of Hcy, Cr and BUN were lower in the intervention group than in the placebo group( t=1.284, 0.779 and 2.541, P=0.016, 0.000 and 0.005). There was no significant difference in plasma Hb level between the two subgroups before and after treatment( F=113.764, P=0.182). There was no significant difference in the renal function staging between the two subgroups before treatment( χ2=4.263, P=0.119), while it was improved in the intervention group versus in the placebo group at the end of follow-up( χ2=73.599, P=0.000). Conclusions:The high level of plasma Hcy can be regarded as an independent risk factor for renal insufficiency, and has a correlation with the renal disease progression and prognosis in elderly patients with CHD.

10.
Article in Chinese | WPRIM | ID: wpr-869264

ABSTRACT

Objective:To evaluate the effectiveness and feasibility of health management based on cloud platform for population with high-risk of coronary heart disease (CHD).Methods:In this study, self-control method was used to study the high-risk group of CHD in two community outpatient clinics in Jinshui District, Zhengzhou through cloud platform. One year later, the clinical indicators, the degree of mastering CHD prevention knowledge andthe medicine compliance of the group were evaluated. Finally, the feasibility and acceptance of cloud platform management were evaluated through questionnaire survey.Results:A total of 272 people were enrolled intothe group. After one year of cloud platform management, the blood lipid, uric acid, homocysteine and fasting blood glucose in the group weresignificantly improved (all P<0.05), but the glycosylated hemoglobin was not significantly reduced [(6.4±1.2)% vs.(6.3±1.1)%, P>0.05]. The degree of mastering CHD prevention knowledgewas significantly improved, and the medicine compliance was not significantly improved [(5.0±1.6) vs. (5.0±1.5), P>0.05]. Questionnaire survey showed that 228 (83.8%) of the patients had a high acceptance of cloud platform management, and 208 (76.5%) of the group wanted to continue to use cloud platform for health management. Conclusions:Health management based on cloud platform can improve the clinical index control of the high-risk population of CHD, and help the high-risk population to master the relevant knowledge of CHD prevention. Some functions of cloud platform need to be further improved.

11.
Article in Chinese | WPRIM | ID: wpr-868535

ABSTRACT

Objective:To compare the differences of fluoroscopy time and dose between HIS bundle pacing and right ventricle apex pacing.Methods:This study includes thirty patients undergoing HIS bundle pacing (HIS group) and 32 patients undergoing right ventricular apex pacing (RVA group). The fluoroscopy time and cumulative dose (CD) to patients during surgery were recorded and analyzed.Results:The operation time for patients in HIS group and RVA group were (76.8±13.1) and (66.0±10.8) min ( t=3.386, P<0.001), respectively. The fluoroscopy time was (698.2±113.7) and (293.3±63.9) s ( t=14.709, P<0.001) and the CD were (391.3±70.0) and (162.3±40.5) mGy ( t=13.694, P<0.001) in HBP group and RVA group, respectively. In comparison, the fluoroscopy time and CD for HIS bundle electrode implantation were (501.2±112.3) s and (279.9±65.0) mGy, respectively, significantly higher than in the case of RVA, where the values were (103.4±30.6) s and (57.3±13.8) mGy ( t=15.864, Z=-6.524, P<0.001). Conclusions:Compared with right ventricular apical pacing, the HIS bundle pacing takes longer operation time, leading to higher radiation dose, which should be prudently selected.

12.
Article in Chinese | WPRIM | ID: wpr-866426

ABSTRACT

Objective:To understand the knowledge situation of sudden cardiac death (SCD) among general practitioners (GPs) and provide the suggestions on the prevention and treatment of SCD basis for GPs.Methods:A self-designed questionnaire on SCD-related knowledge was used for GPs in He′nan province, including GPs in the training program and working in rural areas.The questionnaire included three dimensions of SCD-related knowledge on prevention, early diagnosis and emergency treatment.There were 5 questions for each dimension, with a total score of 15 points for all 15 questions.Statistical analysis was performed using t test, analysis of variance and Chi-square test. Results:A total of 124 GPs were included in the cross-sectional survey, including 63 GP trainers and 61 rural GPs.The GPs had the low level of SCD-related knowledge of prevention, early diagnosis and emergency treatment, with an overall score of 8.03 (SD=2.58). The score of GP trainers was significantly higher than that of rural GPs in all three dimensions [(3.44±0.89)points vs.(2.19±1.06)points, (2.67±0.91)points vs.(1.43±0.80)points, (3.54±0.94)points vs.(2.84±1.30)points, t=7.13, 8.11, 3.45, all P<0.001]. Rural GPs had a significantly higher correct rate of " choice of early screening examinations" and " first-aid measures for cardiac arrest" than GP trainers (50.8% vs.9.8%, 88.9% vs.26.2%, χ 2=24.79, 49.02, all P<0.001). Conclusion:GPs have low levels of SCD-related knowledge.The training for the GPs should be targeted on the weak points of knowledge on the primary prevention and secondary prevention of SCD.

13.
Article in Chinese | WPRIM | ID: wpr-864265

ABSTRACT

On May 8, 2020, for the first time, European enhanced recovery after surgery(ERAS) society lunched Consensus Guidelines for Perioperative Care in Neonatal Intestinal Surgery, that provided 17 suggestions for intestinal resection surgery, including the selection of surgical method, the application of prophylactic antibiotics, the prevention of intraoperative hypothermia, perioperative fluid management, perioperative pain management, optimal hemoglobin, perioperative communication, parents involvement, postoperative nutrition care and mucous fistula refeeding.The guideline aims to recommend ERAS advices for neonatal intestinal surgery, so as to reduce perioperative adverse events, and improve medical quality as well as family satisfaction.Now, in order to provide the latest evidence-based information for the perioperative management practice of neonatal surgery in China, the author interpreted it.

14.
Article in Chinese | WPRIM | ID: wpr-861904

ABSTRACT

Objective: To compare Asian Oncology Summit (AOS, 2016) criteria and liver imaging reporting and data system (LI-RADS) in diagnosis of hepatitis B inflammatory small hepatocellular carcinoma(sHCC). Methods: Upper abdominal CT and MR examination were performed in 130 patients with suspected hepatitis B inflammatory sHCC (172 hepatic nodules with diameters ≤30 mm). Taken pathological results as the gold standards, the sensitivity and specificity of AOS standard and LI-RADS for diagnosis of hepatitis B sHCC were calculated. Results: ①For lesions with diameter ≤30 mm, the sensitivity and specificity of AOS criteria was 55.00% (44/80) and 92.39% (85/92), while of Li-RADS grading criteria was 100% (80/80) and 67.39% (62/92), respectively. ②For lesions with diameter <20 mm, the sensitivity and specificity of AOS criteria was 40.00% (10/25) and 94.34% (50/53), of Li-RADS grading criteria was 100% (25/25) and 71.70% (38/53), respectively. Conclusion: LI-RADS was more sensitive in diagnosis of hepatitis B inflammatory sHCC than AOS criteria, being helpful to early diagnosis and treatment.

15.
Article in Chinese | WPRIM | ID: wpr-828128

ABSTRACT

Rapid development is undergoing in the field of rehabilitation robots, and more countries (regions) are participating in international cooperation and becoming academic contributors. Here in this study, the bibliometric method is used to determine the dynamics and developments of international cooperation in China. The publication data are indexed in Web of Science with search term of rehabilitation robot from 2000 to 2019. Compared with other countries (regions), publication with international co-authors and institutes participating in international cooperation are studied by assessment of indicators such as the cooperation degree, cooperation frequency, and the frequency of citations. The results show that in the past two decades, international scientific cooperation has shown a positive tendency in China, and participating in international collaboration could improve China's impact on the global rehabilitation robot. The United States, England and Japan are the top three countries in number of cooperation with China. Our findings provide valuable information for researchers to better understand China's international scientific collaboration in rehabilitation robot.


Subject(s)
Bibliometrics , China , International Cooperation , Medicine , Time Factors , United States
16.
Article in Chinese | WPRIM | ID: wpr-756400

ABSTRACT

Objective Establish the post cardiovascular surgery SOFA score( pcvsSOFA) based on the Sequential Organ Failure Assessment( SOFA) score to evaluate the severity and prognosis of patients after cardiovascular surgery. Methods 3872 consecutive patients who underwent cardiovascular surgery and stayed in ICU more than 24 hours between July 2015 and December 2017 were retrospectively analyzed to establish a derivation cohort. Univariable and multivariable logistic regression were used to identify the predictors in the pcvsSOFA. And the validity of the new model was evaluated in the derivation cohort and validation cohort. Results Respiratory system score, coagulation system score, nervous system score and renal system score on the first day of ICU, Emergency surgery and re-surgery were risk factors and established the pcvsSOFA. The total score of pcvsSOFA was 20 point and was divided into four classes, namely class Ⅰ( pcvsSOFA-score:1-5 ) , class Ⅱ( pcvsSOFA-score:6-10 ) , class Ⅲ( pcvsSOFA-score:11-15 ) and class Ⅳ( pcvsSOFA-score:16-20 ) with their corresponding predicted mortality0.9%(n=2317),5.7%(n=1367),26.8%(n=184),and64.2%(n=4) inthederivationcohort. Thearea under the receiver operating characteristics curve(AUC)was 0. 864(95%CI:0. 837-0. 892), and the integrated discriminant improvement(IDI) was 0. 035. In the validation cohort, AUC(pcvsSOFA) = 0. 832(95%CI:0. 735-0. 928), IDI=0. 211. The AUC of SOFA, APACHE Ⅱ and SAPS Ⅱ was 0. 771, 0. 793 and 0. 721 respectively. Conclusion Compared with the SOFA score, pcvsSOFA could be a better tool to assess the prognosis of critical patients in the early postoperative stage.

17.
Chinese Journal of Cardiology ; (12): 640-646, 2019.
Article in Chinese | WPRIM | ID: wpr-805712

ABSTRACT

Objective@#To investigate the impact of n-3 polyunsaturated fatty acid (n-3 PUFA) on function and expression of store-operated calcium channels (SOCC) in coronary artery smooth muscle cells (SMC) derived from diabetic rat.@*Methods@#A total of 180 healthy male Sprague-Dawley (SD) rats were randomly divided into normal group (N, n=45), placebo-treated diabetic group (D, n=45), lose dose n-3 PUFA treated diabetic group (DL, n=45) and high dose n-3 PUFAs treated diabetic group (DH, n=45). Streptozotocin-induced diabetic rat animal model was established by two consecutive intraperitoneal injections. After modeling, rats in group DL and DH were treated with 10 mg·kg-1·d-1 and 50 mg·kg-1·d-1 n-3 PUFAs respectively per gavage for eight weeks. After eight weeks, rat coronary artery SMC was isolated by enzyme digestion. Changes of cytosolic calcium concentration in coronary artery SMC were examined by calcium fluorescence imaging technique, coronary artery tension was detected by myograph system, and protein expressions of SOCC on coronary artery SMC were measured by Western blot.@*Results@#SOCC induced ΔF340/F380 of group N, D, DL and DH were 0.425±0.023, 0.838±0.037, 0.342±0.052 and 0.364±0.045 respectively, which was significantly lower in group N, DL, DH than in group D (P<0.05). SOCC induced changes of tensions were 0.94±0.09, 1.95±0.18, 1.35±0.24 and 1.01±0.18 in the group N, D, DL and DH, respectively, which was significantly lower in group N and DH than in group D (P<0.05). Protein expressions of STIM1, Orai1 and TRPC1 were significantly higher in diabetic rat coronary SMC than in group N (P<0.05). STIM1 protein expressions were significantly lower in group DL and DH than in group D, and Orai1 and TRPC1 protein expressions were similar among group.@*Conclusions@#Coronary artery tension, cytosolic calcium concentration and protein expressions of SOCC are higher in diabetic rat coronary artery SMC when compared with normal rats. n-3 PUFA intervention could downregulate the protein expression of SOCC, reduce cytosolic calcium concentration and coronary artery tension, and is protective to the diabetic injury in coronary artery.

18.
Chinese Journal of Dermatology ; (12): 319-322, 2019.
Article in Chinese | WPRIM | ID: wpr-745788

ABSTRACT

Objective To evaluate the value of modified calcofluor white fluorescent staining in the histopathological diagnosis of subcutaneous mycosis,in order to provide a new method for histopathological diagnosis of subcutaneous mycosis.Methods A total of 21 lesional skin tissues were collected from patients with subcutaneous mycosis in the Affiliated Hospital of Chengde Medical University between 1987 and 2017,and embedded in paraffin.Then,each paraffin-embedded tissue section was cut into 4 4-μm-thick serial sections,and subjected to modified calcofluor white fluorescent staining,hematoxylin and eosin (HE) staining,periodic acid Schiff (PAS) staining and Gomori methenamine silver nitrate (GMS) staining respectively.Positive rates and staining outcomes were compared among the above staining methods.Statistical analysis was carried out with SPSS 19.0 software by using chi-square test for comparing the positive rates among the above 4 staining methods.Results Of 21 patients with fungal infections,14 (66.67%) were positive for modified calcofluor white fluorescent staining,5 (23.80%) for HE staining,6 (28.57%) for PAS staining,and 11 (52.38%) for GMS staining.The positive rate by modified calcofluor white fluorescent staining was significantly higher than that by HE staining and PAS staining (x2 =6.718,5.200,respectively,both P < 0.05),while no significant difference was observed between the modified calcofluor white fluorescent staining and GMS staining (x2 =0.693,P =0.530).Conclusion The modified calcofluor white fluorescent staining is an accurate method for detecting fungi,and has a certain application value in the histopathological diagnosis of subcutaneous mycosis.

19.
Acta Pharmaceutica Sinica B ; (6): 1050-1060, 2019.
Article in English | WPRIM | ID: wpr-774923

ABSTRACT

Chemotherapy is among the limited choices approved for the treatment of hepatocellular carcinoma (HCC) at intermediate and advanced stages. Preferential and prolonged drug exposure in diseased sites is required to maximize the therapeutic index of the drug. Here, we report an injectable supramolecular peptide hydrogel as an intraperitoneal depot for localized and sustained release of triptolide for the treatment of orthotopic HCC. We chose peptide amphiphile C-GNNQQNYKD-OH-based nanofibers as gelators and carriers for triptolide. Sustained triptolide release from the hydrogel was achieved over 14 days , with higher accumulation in and cytotoxicity against human HCC Bel-7402 in comparison with L-02 fetal hepatocytes. After intraperitoneal injection, the hydrogel showed prolonged retention over 13 days and preferential accumulation in the liver, realizing HCC growth inhibition by 99.7 ± 0.1% and animal median survival extension from 19 to 43 days, without causing noticeable pathological changes in the major organs. These results demonstrate that injectable peptide hydrogel can be a potential carrier for localized chemotherapy of HCC.

20.
Journal of Medical Biomechanics ; (6): E637-E643, 2019.
Article in Chinese | WPRIM | ID: wpr-802405

ABSTRACT

Objective To improve the clinical application of using rehabilitation robot for hand rehabilitation and solve the current shortcomings of rigid hand rehabilitation robot, such as complex structure, heavy weight, potential safety hazard, a new soft and wearable robotic glove was proposed. Methods The robotic glove was driven by McKibben pneumatic artificial muscles (PAMs). The tendon drive system was designed based on simulation of human hand anatomy and physiology structure, which could transmit forces and torques through the user’s own skeleton and joints. The normal hand movement could be simulated and this design pattern highly reduced the weight of the robotic glove. Meanwhile a surface electromyogrphy (sEMG) collecting circuit was developed to acquire sEMG signals from the forearm. User intent could be detected by measuring the sEMG of flexor digitorum superficialis and extensor digitorum communis on the forearm. Results The results of the experiment investigation on characteristics of the soft robotic glove showed that the robotic glove could effectively assist people completing daily activities and grasping daily necessities. The feasibility and scientificity of the robotic glove was validated. Conclusions The soft and wearable robotic glove has an advantage of light weight, easy operation and high comfortableness, and it can provide references for the study and design of similar hand rehabilitation devices.

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