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Objective To screen key genes and pathways of acute on-chronic liver failure(ACLF)by multiple bioinformatics,and to provide theoretical basis for molecular biology studies and biomarker screening of ACLF.Methods ACLF transcriptome mRNA mi-croarray data set was downloaded from Gene Expression Omnibus(GEO),and limma package in R software was used to analyze the difference expression genes.The gene ontology(GO)function enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)anal-ysis were analyzed differential genes through David database.Protein-protein interaction(PPI)network was analyzed using STRING da-tabase,the key differential genes were screened by Cytoscape software.Results A total of 329differentially expressed genes were screened,including 185 up-regulated genes and 144 down-regulated genes.GO functional enrichment analysis obtained 385 items,in-cluding immune receptor activity,cytokine receptor activity,T cell receptor binding and other biological functions(P＜0.05).KEGG pathway enrichment analysis screened 36signaling pathways,among which the immune and inflammatory pathways including Th1 and Th2 cell differentiation,Th17 cell differentiation pathway,T cell receptor signaling pathway,primary immune deficiency,NF-κB signaling pathway and TNF signaling pathway.Among these key genes,CD3G,CD3D,IL7R,LCK,IL1R2,IL18R1,IL1R1 and MAPK14 related to ACLF were further obtained,which may become potential biomarkers and therapeutic targets of ACLF.Conclusion This study demon-strates that CD3G,CD3D,IL7R,LCK,IL1R2,IL18R1,IL1R1 and MAPK14may become the core genes related to the occurrence and development of ACLF and new therapeutic targets in the future.
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It analyzes the significance and value of prioritizing health development,explains the basic connotation and core meaning of prioritizing health development,and puts forward the framework of the system of prioritizing health development.It proposes that health priority development is a collection of a series of ideas,systems and development models that arrange health as a priority in a country's economic and social development.The system of health priority development refers to a unified whole composed of all kinds of systems or behavior rules that are conducive to promoting and protecting residents'health.Healthy civilization,healthy promotion society and health impact assessment system are building blocks of system construction.
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Objective To analyze the dynamic flow of pediatricians from 2010 to 2015, their present deployment and workload as compared with physicians across the country, in order to provide reference for their effective allocation.Methods Selecting data indexes from Chinese Statistical Yearbook and China Health and Family Planning Yearbook, and using Excel for statistics of relevant index data.By means of horizontal and vertical comparisons, a dynamic analysis was made on such indexes as the number of pediatricians, that of pediatricians per thousand children, and the number of hospital admissions and the number of hospitalizations per pediatrician.Results The total of pediatricians only increased by 2.32% from 2010 to 2015.On the other hand, the number of pediatricians per thousand population only accounted for 23.42% of physicians per thousand population in 2015.These five years witnessed a 40.58% workload increase of outpatient/emergency visits per pediatrician, and 36.70% increase of discharged inpatients.Conclusions China is faced with the challenges of shortage and insufficient supply of pediatricians, worsened by excessive workloads.It is advisable to improve pediatrician development pattern, raise their remuneration, and ease their workload, for the sake of better healthcare for children.
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Objective To determine main factors associated with quality of life in epileptics in northen China especially seizure severity and stigma, and the variations between men and women. Methods All patients with epilepsy aged 18-65 years were selected from the First Affiliated Hospital of China Medical University, tested from March 2014 to March 2015. All of them completed the general social science data, the Quality of Life Inventory in Epilepsy-31 (QOLIE-31), Liverpool Adverse Events Profile (LEAP), the National Hospital Seizure Severity Scale (NHS3), Epilepsy Stigma Scale (ESS), Self-rating Anxiety Scale ( SAS) and Hamilton Depression Scale ( HAMD) assessment. Results A total of 123 patients with epilepsy (60 men, 63 women) were interviewed. QOLIE-31 scores were 55. 81 ± 10. 17 in female patients, 65. 35 ± 10. 89 in male patients, the difference being statistically significant (t=2. 90, P<0. 05). ESS (1(0,2)), SAS(31. 85 ± 8. 16), HAMD (4. 38 ± 2. 90) scores of female patients were higher than that of male patients (0(0,0), 26. 93 ± 3. 45, 2. 80 ± 1. 39;U= -2. 710, t=2. 48, 2. 20, all P<0. 05). Multiple factors analysis and multiple stepwise regression analysis on quality of life revealed that depression (β= -0. 516, P<0. 01) and the severity of epilepsy (β= -0. 338, P=0. 01) were two strong predictors of QOLIE-31 overall scores. The linear regression showed that there was a direct link between shame and degree of depression (β = 0. 515, P= 0. 001 ) . Conclusions The main factors affecting the quality of life in epileptics in northen China are depression and seizure severity. Compared with male patients, female patients have a lower quality of life, and poorer psychological status, such as shame, depression and anxiety. There is a direct link between shame and depression in epileptics.
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@#A long chain structure of DNA(polyaptamer)composed of multiple aptamer units was synthesized by rolling circle amplification and used for the construction of polyaptamer-doxorubicin system in the treatment of leukemia cells. It was found that the system was significantly more effective than monoaptamer in targeting and killing leukemia cells as it provided 35-fold enhanced binding affinity and 10-fold greater drug loading via multivalent effects. Drug release and cell viability also proved that the conjugates could gain entrance into the cells and rapidly release doxorubicin under the action of lysosome, leading to the tumoricidal effect.
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On the basis of drawing on recently published literature on international medical tourism, this paper comprehensively summarizes the effects thereof on driving economic growth, expanding employment and improving in-ternational competitive power, analyzes the main driving forces for the international medical tourism development and the experiences from Thailand, India, Malaysia, Japan, South Korea, etc. According to China’s advantages and dis-advantages and learning from the international experience, this paper proposes suggestions to develop China’s interna-tional medical tourism, including an improvement to the management system, formulating international medical tourism development strategies, building brand characteristics with traditional Chinese medicine, constructing ‘Ex-perimental and Leading Area’ , and promoting international certification for the medical institutions and services.
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Chinese ethics is a science which takes a wide range of knowledge as the goal of life and changes benevolence,uprightness,ceremony,wisdom and faith into methods to achieve the goal.Advanced medical science and technology is wisdom crystallization of research worker.
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on the basis of systematic examination to the policy system of private medical institutions, this paper summarize the current situation and main problems of the policy system, including basic concepts, related issues a-bout the non-state owned enterprise management, important policies about the non-profit medical institutions, the ab-sence of some main policies. To solve these problems, this paper puts forward specific suggestions, such as to speed up the legislation, to make standards of non-profit medical institutions judging and qualification of exempt from tax in details, to construct the reasonable return system for the investors, to extend the tax holidays for the profit-making medical institutions, to make sure private medical institutions have equitable access to qualification in pilot hospital of healthcare insurance, to improve the transformation mechanism between Non-profit and profit-making institutions, to formulate specific measures.
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Objective:To analyze the characteristics of health financing at the provincial level according to the total health expenditure since China health system reform began in 2009 and provide evidence for improving health fi-nancing policy. Methods:20 provinces were chosen and vertical and horizontal Comparative approach was used to an-alyze the data. Results:Total health expenditure increased for all regions, of which the biggest rate was Anhui prov-ince, about 82. 97%, while the largest increasing for government health care expenditure was Ningxia province, a-bout 108 . 71%. In 2012 , the provinces with social health expenditure share of total above 40% were allocated in the east region, and the number of provinces with out-of pocket payment share of total above 40% reduces to 5. Conclu-sion:Total health expenditure grew in all regions, but there were differences in the degree that this spending matched the economic level;The financing structure was optimized, but the characteristic of regional financing was different. Some provinces were under huge pressure to reduce out-of pocket payments. Suggestions: Under the premise of im-proving the funding level, financing structure adjustment must be focused, and public funding should play a bigger role and out-of pocket payments should be reduced.
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The theory of rough set was applied to the law of compatibility of categorized formulas research, and the decision rules model of the law of compatibility of categorized formulas was set up in order to explore the correlation between the compatibility of the categorized formula and the syndrome indexes. Based on this model, the core herbs of categorized formula were obtained through attribute reduction, the interaction of each herb in categorized formula could be analyzed by the effective decision rules, and the efficacy of the categorized formula could be predicted by decision rules. It would be very significant to optimize the compatibility of categorized formula and guide the clinical-practice.
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Drug Interactions , Drug Prescriptions , Drug Therapy, Combination , Drugs, Chinese Herbal , Medicine, Chinese Traditional , Methods , Models, TheoreticalABSTRACT
Prediction on pharmacodynamic action of categorized formulas is presented with the theory of support vector regression (SVR) in this paper. A prediction model of pharmacodynamic action of categorized formulas based on SVR was set up in order to predict the law of the compatibility of the categorized formulas. Pharmacodynamic action of various categorized formulas could be predicted based on this model. It is very significient to optimize the compatibility of categorized formulas and clinical practice. This model was applied to the research of the law of compatibility in three categorized formulas for tonifying kidney yang which contains shenqi pill, yougui pill and yougui drink. As indicated in the model prediction, pharmacodynamic actions of several compatibilities of the categorized formulas are superior to that of the three original formulas for tonifying kidney yang.
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Algorithms , Drug Synergism , Drug Therapy, Combination , Drugs, Chinese Herbal , Models, Theoretical , Support Vector MachineABSTRACT
Objective To study the efficacy arid side effects of postoperative analgesia between Lornoxicam and Buprenorphine. Methods Sixty ASA I-II patients were randomly divided into Lomoxicam group(group A) and Buprenorphine group(group B). Analgesiae were subcutaneously injected via an electronic patients controlled analgesic pump post surgeries. The visual analgesiae sacle( VAS) was used to evaluate the postoperative pain relief,and analgesic response was recorded. Result There were no significant differences between Lomoxicam and Buprenorphine about the analgesic response(P>0. 05),moreover,no stastical differences in the patients demography,analgesic and anesthetic type were associated with the analgestic outcomes. Conclusion Lomoxicam without respiratory distuber-ance was a good alternative to Buprenorphine in PCSA.
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Objective To study the efficacy and side effects of tramadol combined with and fentanyl for patient-controlled analgesia(PCA) after pancreatectomy. Methods 30 ASA Ⅰ-Ⅱ patients were randomly divided into tramadol group (group A) and droperidol group ( group B). Results Sedation score in group B at 24 h was significantly higher than that in group A(P ＜0. 05). Side-effects were significantly more in group B than those in group A. Conclusion The analgesia efficacy of tramadol combined with and fentanyl was effective and safe with less side effects than Droperidol
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Aim To explore the effect of Baicalein and its mechanism on the invasion and migration of human breast cancer MDA-MB-231 cells.Methods The effect of Baicalein on cell viability was tested by MTT.The cell invasion and migration were determined by transwell chamber model.The cell movement capability was determined by cell scratch assay.The expressions of matrix metalloproteinase(MMP)2 and 9,urokinase-type plasminogen activator(uPA)were detected by Western blot.Results 50,100 ?mol?L-1 of Baicalein inhibited significantly cell invasion(P