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Chinese Journal of Epidemiology ; (12): 426-429, 2007.
Article in Chinese | WPRIM | ID: wpr-294323

ABSTRACT

<p><b>OBJECTIVE</b>To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.</p><p><b>METHODS</b>1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.</p><p><b>RESULTS</b>The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.</p><p><b>CONCLUSION</b>The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.</p>


Subject(s)
Animals , Bacterial Proteins , Genetics , Allergy and Immunology , Enzyme-Linked Immunosorbent Assay , Mice , Plague , Microbiology , Polymerase Chain Reaction , Yersinia pestis , Genetics , Allergy and Immunology , Virulence
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