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1.
Chinese Medical Journal ; (24): 2611-2618, 2021.
Article in English | WPRIM | ID: wpr-921137

ABSTRACT

BACKGROUND@#Nucleolar protein 6 (NOL6) is a nucleolar RNA-associated protein that is highly conserved between species. It has been proved to be associated with the prognosis of liver cancer. However, the underlying mechanism has not been fully established. This study aimed to assess the relationship between NOL6 and liver cancer prognosis.@*METHODS@#We constructed an NOL6-short hairpin RNA (shRNA)-expressing lentivirus. Through viral transfection, cell growth assay and fluorescence-activated cell sorting, we evaluated the effect of shRNA-mediated NOL6 knockdown on the proliferation, colony formation, and apoptosis of hepatocellular carcinoma (HCC) cells. The relationship between NOL6 expression and HCC patient survival has been established through bioinformatics analysis. We also explored the downstream molecular regulatory network of NOL6 in HCC by performing an Ingenuity Pathway Analysis in the database.@*RESULTS@#Increased NOL6 expression was detected in HCC cells compared to normal controls; HCC patients with high NOL6 expression had poorer prognoses than those with low expression. NOL6 knockdown inhibited HCC cell proliferation, apoptosis, and colony formation. Also, MAPK8, CEBPA, and FOSL1 were selected as potential downstream genes of NOL6.@*CONCLUSIONS@#NOL6 up-regulates HCC cell proliferation and affects downstream expression of related genes. Moreover, NOL6 is considered to be associated with poor prognosis in HCC patients.


Subject(s)
Apoptosis/genetics , Carcinoma, Hepatocellular/genetics , Cell Line, Tumor , Cell Proliferation/genetics , Gene Expression Regulation, Neoplastic , Humans , Liver Neoplasms/genetics , Nuclear Proteins , Prognosis
2.
Chinese Acupuncture & Moxibustion ; (12): 1109-1112, 2021.
Article in Chinese | WPRIM | ID: wpr-921018

ABSTRACT

OBJECTIVE@#To observe the effect of moxibustion combined with @*METHODS@#A total of 240 close contacts of COVID-19 were randomized into an observation group (120 cases, 18 cases dropped off) and a control group (120 cases, 58 cases dropped off). Conventional observation was adopted in the control group. Moxibustion combined with Daiwenjiu plaster was given in the observation group, moxibustion was applied at Zusanli (ST 36), Hegu (LI 4) and Shenque (CV 8), 10 min each acupoint, once a day; @*RESULTS@#In the follow-up, SRQ-20 score was decreased compared before treatment (@*CONCLUSION@#Moxibustion combined with


Subject(s)
Acupuncture Points , COVID-19 , Humans , Mental Disorders , Moxibustion , SARS-CoV-2
3.
Article in Chinese | WPRIM | ID: wpr-799206

ABSTRACT

Primary and secondary airway problems directly affect mortality and disability in critically ill children in pediatric intensive care unit(PICU). Soft bronchoscope, as an important method for the diagnosis and interventional treatment of airway lesions in critically ill children in PICU, requires standardized seamless nursing cooperation throughout the whole process.This article discussed the nursing cooperation before, during and after soft bronchoscope operation in PICU.

4.
Article in Chinese | WPRIM | ID: wpr-799205

ABSTRACT

Children in the pediatric intensive care unit who continue to have ventilator-assisted breathing are often difficult to get off-line due to respiratory system disease, cardiovascular system disease, nervous system disease, nutritional status, genetic metabolism, abnormal diaphragm movement and other factors.Through the diagnosis and treatment of flexible bronchoscope, the etiology could be identified, the ventilation function is effectively improved, and the cure rate of weaning difficulty increases.This paper discussed the etiological diagnosis and treatment of flexible bronchoscopy in children with difficulty in weaning from pediatric intensive care unit.

5.
Article in Chinese | WPRIM | ID: wpr-799202

ABSTRACT

Tracheobronchial foreign body aspirations may cause cardiopulmonary arrest and sudden death.The incidence in children is higher than in adults.Rapid diagnosis and treatment are live saving.In this paper, we aimed to present our experience in tracheal foreign body aspirations by fiberoptic bronchoscopy.

6.
Article in Chinese | WPRIM | ID: wpr-864898

ABSTRACT

Objective:To investigate the characteristics of sublingual microcirculation in children with sepsis, and to explore the clinical value of sublingual microcirculation in the early diagnosis, therapeutic effect evaluation of sepsis in children.Methods:Children with sepsis and community acquired pneumonia(CAP) admitted in our hospital from December 2018 to December 2019 were included in the study, which was divided into sepsis group and control group(CAP group). In the sepsis group, large circulation indexes and sublingual microcirculation indexes were collected at 0, 6, and 24 hours after admission.Sublingual microcirculation indexes were collected by bypass dark field imaging.In the control group, large circulation indexes were collected after admission, and sublingual microcirculation indexes were collected during tracheal endoscopic examination after sedation.De Backer score(DBs), hetergeneity index(HI), hetergeneity index small(HIs), proportion of pefusedvessels(PPV), proportion of pefused small vessels(PPVs), total of vessel density(TVD), pefused vessel density(PVD), pefused small vessel density(PVDs)were selected as the evaluation index of sublingual microcirculation.The changes of macrocirculation and microcirculation indexes in the two groups were compared, and the correlation between age and microcirculation indexes in the control group was analyzed.Results:A total of 71 children, including 10 sepsis cases and 61 CAP cases, were collected.Among the 61 children with CAP, 9 children (2-48 months) in the same age range as the sepsis group were divided into control group A, and the indicators between the two groups were analyzed.Compared with control group A, PPV and PPVs were lower and DBs, HI, HIs, TVD were higher in the sepsis group at 0 hours( P<0.05). At 6 hours, HI in the sepsis group was still higher than that in control group A, and PPV, PPVs were higher than those at 0 hours.However, at 6 hours, PPVs was still lower than that of control group A( P<0.05), and HI, HIs did not improve compared with those at 0 hours( P>0.05). At 24 hours, HI, PPV and PPVs improved, no difference was found compared with the control group A( P<0.05), while HIs did not improve significantly( P>0.05). The age of the control group had a moderate negative correlation with HI, and a weak negative correlation with HIs( r=-0.420, P=0.001; r=-0.387, P=0.002). Conclusion:HI, HIs, PPV and PPVs are sensitive indexes of microcirculation disorder in children with sepsis.Fluid resuscitation therapy can improve the indexes of sublingual microcirculation in children with sepsis, but the improvement of sublingual microcirculation in children with sepsis is later than the recovery of macro circulation.HI and HIs are negatively correlated with month age in children with CAP.

7.
Article in Chinese | WPRIM | ID: wpr-864870

ABSTRACT

Children in the pediatric intensive care unit who continue to have ventilator-assisted breathing are often difficult to get off-line due to respiratory system disease, cardiovascular system disease, nervous system disease, nutritional status, genetic metabolism, abnormal diaphragm movement and other factors.Through the diagnosis and treatment of flexible bronchoscope, the etiology could be identified, the ventilation function is effectively improved, and the cure rate of weaning difficulty increases.This paper discussed the etiological diagnosis and treatment of flexible bronchoscopy in children with difficulty in weaning from pediatric intensive care unit.

8.
Article in Chinese | WPRIM | ID: wpr-864868

ABSTRACT

Tracheobronchial foreign body aspirations may cause cardiopulmonary arrest and sudden death.The incidence in children is higher than in adults.Rapid diagnosis and treatment are live saving.In this paper, we aimed to present our experience in tracheal foreign body aspirations by fiberoptic bronchoscopy.

9.
Article in Chinese | WPRIM | ID: wpr-864865

ABSTRACT

Primary and secondary airway problems directly affect mortality and disability in critically ill children in pediatric intensive care unit(PICU). Soft bronchoscope, as an important method for the diagnosis and interventional treatment of airway lesions in critically ill children in PICU, requires standardized seamless nursing cooperation throughout the whole process.This article discussed the nursing cooperation before, during and after soft bronchoscope operation in PICU.

10.
Article in Chinese | WPRIM | ID: wpr-752249

ABSTRACT

During the renal replacement therapy,the blood coagulation system is activated in the extracorporeal circulation line and the filter,resulting in blood coagulation. This can interrupt treatment and shorten the life of the ex﹣tracorporeal circulation line and filter. The consequences are the effectiveness of renal replacement therapy decrease While increasing Workload and treatment costs. Therefore,appropriate anticoagulation measures are needed to complete this treatment. To ensure the continuity of extracorporeal bypass,but also to monitor,detect and intervene for the compli﹣cations caused by anticoagulation. NoW,the general theory of renal replacement therapy and anticoagulation technology is discussed.

11.
Acta Pharmaceutica Sinica ; (12): 1271-1276, 2019.
Article in Chinese | WPRIM | ID: wpr-780219

ABSTRACT

In this manuscript, a rapid and simple analysis method for aconitine alkaloids was established. The method was based on the use of direct ionization and wooden tip spray ionization technology to detect the aconite. The aconite tuber slices were wrapped with wet filter paper overnight, cut into triangles, and extracted with a few solvents for direct ionization and wooden tip spray mass spectrometry. The results showed that alkaloids in aconite tuber can be rapid detected by two mass spectrometric methods without tedious sample pretreatment. Both methods are superior to that of traditional capillary electrospray ionization mass spectrometry (ESI-MS). The direct ionization MS is better than the wooden tip spray MS for analysis of aconite except under the condition of methylene chloride as extract or spray solvent. Different types of alkaloids in aconite tuber can be selectively detected when different solvents are used. The experiments provide a rapid and no pretreatment MS spectrometric method for analysis of alkaloids in aconite. These sample methods are important for research on aspects of plant varieties, storage, prescription compatibility, and quality control of aconite.

12.
Journal of Medical Postgraduates ; (12): 1174-1178, 2019.
Article in Chinese | WPRIM | ID: wpr-818162

ABSTRACT

Objective Influenza A (FluA) poses a serious threat to human health due to its high infectivity, viral variation and rapid progression. This study aimed to analyze the characteristics of T-lymphocyte subsets in patients with severe FluA and their role in the prognosis of the disease. Methods We collected the clinical data on 80 cases of severe FluA treated in our Department of Respiratory and Critical Diseases from January 2018 to May 2019, which were divided into a death group (n = 25) and a survival group (n = 55) based on 28-day survival after admission. Thirty-one of the patients were co-infected with other pathogens at admission, of whom 11 died and 20 survived within 28 days after admission. We compared the clinical characteristics, general laboratory results and T-lymphocyte subsets (CD3+T, CD4+T and CD8+T cells) between different groups and analyzed the factors influencing the prognosis of the disease using logistic regression and multivariate COX regression analyses. Results Compared with the patients in the survival group, those in the death group showed significantly decreased counts of lymphoid, CD3+T, CD4+T and CD8+T cells, albumins and globulins (P < 0.05), but increased levels of procalcitonin, blood urea nitrogen, serum creatinine and serum glutamic-oxaloacetic transaminase (P < 0.05). CD3+T, CD4+T and CD8+T cells and albumins were remarkably decreased in the co-infection death group in comparison with the co-infection survival group (P < 0.05). The area under the ROC curve for the count of CD4+T cells was 0.728 (95% CI: 0.605-0.850), with the best cut-off value of 1.70 × 108/L, a sensitivity of 80% and a specificity of 64%. The 28-day survival rate was significantly higher in the patients with CD4+T cells ≥ 1.70×108/L than in those with CD4+T cells < 1.70×108/L (83% vs 41%, P < 0.05). COX regression analysis showed age, respiratory failure and alanine transaminase to be the risk factors while the count of CD4+T cells (RR = 0.740, 95% CI: 0.572-0.958) to be a preventive factor for the death of the patients within 28 days after admission (P < 0.05). Conclusion The count of T-lymphocytes are closely correlated to the prognosis of severe FluA and the patients with a decreased count of CD4+T cells have a higher risk of death within 28 days after admission. The count of CD4+T cells can predict the 28-day prognosis of severe FluA.

13.
Article in Chinese | WPRIM | ID: wpr-774001

ABSTRACT

Objective To analyze the levels of cytokines (IL-2,IFN-γ,IL-6,IL-10) associated with Th1 and Th2 cells in HLA-DQ8 transgenic mice model of ocular experimental autoimmune myasthenia gravis (oEAMG) induced by recombinant H-AChR γ subunit immunization.Methods DQ8 mice were immunized with 20 μg of AChR γ subunit,20 μg of crude E. coli extract (E. coli group),or complete Freund's adjuvant (CFA) only (CFA group). All mice were immunized on days 0,30,and 60. Mice were euthanized 28 days after the third immunization,and draining lymph node cells (LNC) and spleen lymphocytes were cultured in vitro. The supernatant was collected to observe the interleukin(IL)-2,interferon(IFN)-γ,IL-6,IL-10 production by ELISA.Results LNCs and spleen lymphocytes of H-AChR γ subunit-immunized mice exhibited significantly enhanced IFN-γ (F=76.332,P<0.001;F=34.865,P<0.001) and IL-2 (F=42.835,P<0.001;F=38.030,P<0.001),which associated with Th1 cells,as compared to E. coli group and CFA group. There were no significant differences in IL-6 (F=1.325,P=0.284;F=1.935,P=0.166) and IL-10 (F=0.908,P=0.417;F=1.189,P=0.322) levels,which secreted by Th2 cells,among these three groups.Conclusion Th1 cytokines play key roles in the pathogenesis of oEAMG,while the mechanism of Th2 cytokines for oEAMG remains unclear.


Subject(s)
Animals , Cytokines , Escherichia coli , HLA-DQ Antigens , Interferon-gamma , Mice , Mice, Transgenic , Receptors, Cholinergic , Th1 Cells , Th2 Cells
14.
Article in Chinese | WPRIM | ID: wpr-776040

ABSTRACT

Retinopathy of prematurity(ROP)is a pathological neovascularization with fibrotic changes in the fundus of premature infants.It is a major cause of preventable blindness in children in both developing and developed countries.Treatment of ROP has long been a hot research topic in ophthalmology and pediatrics.With a clearer knowledge of the pathogenesis of ROP,more basic and clinical studies have been carried out.The anti-vascular endothelial growth factor therapy and surgical treatment have become mature strategies,and a variety of therapeutic drugs including insulin-like growth factor-1,transforming growth factor-β,polyunsaturated fatty acids,and β-adrenergic receptor blockers have been developed.This article reviews the recent advances in ROP.


Subject(s)
Child , Humans , Infant, Newborn , Infant, Premature , Neovascularization, Pathologic , Therapeutics , Retinopathy of Prematurity , Drug Therapy , General Surgery , Vascular Endothelial Growth Factor A
15.
Article in Chinese | WPRIM | ID: wpr-776019

ABSTRACT

Ocular myasthenia gravis(OMG)is an autoimmune disease caused by neuromuscular junction transmission disorders and manifested mainly as fluctuating blepharoptosis and diplopia,with the extraocular muscles as the main involveed sites.While the pathogenesis of OMG remains unclear,some antibodies,complements,and cytokines may be the contributing factors.The diagnosis and treatment of OMG have been defined in recent years.This article reviews the pathogenesis,diagnosis,and treatment of OMG.


Subject(s)
Antibodies , Complement System Proteins , Cytokines , Humans , Myasthenia Gravis , Diagnosis , Pathology , Therapeutics , Oculomotor Muscles , Pathology
16.
Chinese Journal of Pediatrics ; (12): 128-133, 2018.
Article in Chinese | WPRIM | ID: wpr-809810

ABSTRACT

Objective@#To investigate the current application status of continuous blood purification (CBP) technology and equipment in pediatric intensive care unit (PICU) in China.@*Methods@#A cross-sectional survey was conducted to understand the current popularization of CBP technology and equipment, the management of CBP equipment and consumables, and the application of CBP in different diseases. A questionnaire named Application Status of Continuous Blood Purification Technology was applied. Children's hospitals and polyclinic hospitals with the pediatric qualification (pediatric emergency or critical care unit members of Chinese Medical Association and Chinese Medical Doctor Association) were selected.@*Results@#From December 2016 to February 2017, 53 hospitals completed the questionnaire, including 7 in northeast, 6 in north China, 16 in east China, 9 in south China, 5 in central China, 4 in the northwest, and 6 in the southwest region. Continuous renal replacement therapy (CRRT), the most widely used technology, was carried out in 51 hospitals. Other technologies were peritoneal dialysis (IPD) (n=37), artificial liver support (ALSS) (n=26) and blood adsorption (PA) (n=13). There were 107 CBP machines in the 51 hospitals used CBP technology, with an average of 2.10/hospital. In 36 hospitals CBP machines were managed independently by PICU (70%). Hospitals made their own displacement liquid (n=40, 78%), or purchased displacement liquid (n=11, 22%). Hospitals prepared dialysate on their own (n=38, 75%), or purchased dialysate (n=13 hospitals, 25%). In 46 (90%) hospitals, hemodialysis catheter was placed independently by PICU doctors. The routine operation and maintenance of CBP were mainly completed by the PICU nurses in 36 hospitals (71%). There were 39 hospitals (76%) where professional nurses manage and maintain CBP. Puncture sites were femoral vein (n=26, 51%), internal jugular vein (n=21, 41%) and venae subclavia (n=4, 8%). Forty-two hospitals (82%) selected B-mode ultrasound positioning and guidance when performing internal jugular vein puncture. A total of 40 (78%) hospitals have developed post dilution and combined dilution techniques during the implementation of CBP. The most common indications of CBP technology were different in different regions. They were sepsis in northeast (24.0%, 243/1 011) and east China region (32.0%, 982/3 069), multiple organ dysfunction syndrome in south China (29.2%, 444/1 520), north China (15.8%, 126/796), and southwest region (30.1%, 460/1 526), drug poisoning in central China region (21.6%, 325/1 506), and renal failure in northwest region (53.0%, 44/83).@*Conclusions@#CBP technology is widely used in the field of pediatric severe diseases in China. The eastern regions possess more CBP equipment than the western regions. CBP is widely used in the treatment of sepsis.

17.
Article in Chinese | WPRIM | ID: wpr-807005

ABSTRACT

Sepsis-associated encephalopathy(SAE), as a relatively common complication of sepsis, is associated with mortality and poor prognosis.Its main symptoms are disturbed cognitive functions and consciousness, including decreased attention, delirium, lethargy, coma, mood changes.At present, the pathogenesis of SAE has not been clearly explained, and the diagnosis and treatment are still controversial.Many of the survivors suffer from long-term cognitive impairment, which imposes heavy burdens on patients, families and society.Therefore, SAE needs to attract the attention of clinicians.

18.
Article in Chinese | WPRIM | ID: wpr-751907

ABSTRACT

Objective To investigate the effectiveness of task-oriented bilateral arm training on upper limb function in children with spastic hemiplegia. Methods 27 children within 4-6 years with spastic hemiplegic were randomly divided into control group (n=13) and experiment group (n=14). The children in control group received routine occupational therapy. The bilateral arm task-oriented training was added for children in experiment group. The Peabody development motor scale-2 (PDMS-2) and Upper Limb Physician's Rating Scale (ULPRS) were used to assess the upper limb function before and after 10 weeks intervention. Results After treatment, the scores of grasping function and visual-motor integration in PDMS-2 were significantly higher than those before treatment (P<0.05), and the scores of experimental group werehigher than that of control group (P <0.05), and the difference of the added value between two groups were statistically significant (P<0.05).The ULPRS scores of both groups increased after treatment (P<0.05), scores between the treatment group and the control group were not statistically significant (P>0.05), and the difference of the added value between two groups was not statistically significant (P>0.05). Conclusion The task-oriented bilateral arm training can promote recovery for upper limb and hand function in children with spastic hemiplegia compared with routine occupational therapy.

19.
Article in Chinese | WPRIM | ID: wpr-692596

ABSTRACT

Sepsis is an infectious disease that affects immune function by various inflammatory factors and is the main cause of death in ICU patients. As a syndrome caused by uncontrollable inflammation,sepsis not only contains a large number of continuous inflammatory reactions,but also has a long-term immunosuppressive state,which increases the probability of secondary nosocomial infection in patients with sepsis. The immune sta-tus of patients with sepsis will change with the progression of the disease,and different biomarkers help to ex-plain the immune stage of patients. Immune-enhancing therapy is expected to reduce the mortality rate of sepsis patients in the stage of immunoparalysis.

20.
Chinese Journal of Biotechnology ; (12): 1338-1345, 2018.
Article in Chinese | WPRIM | ID: wpr-687683

ABSTRACT

Carbapenem-resistant Enterobacteriaceae (CRE) is rising rapidly all over the world, and challenges clinical diagnosis and treatment by various genotypes. This paper summarizes the characteristics and diagnostic methods of CRE. CRE can be divided into three categories and five families with various characteristics and resistant genes. The diagnosis method include the Kirby-Bauer screening test, double disc synergetic inhibition test with carbapenems collaboration (double disc EDTA and meropenem, phenylboronic acid and meropenem), modified Hodge test, chromogenic medium detection for selected test of CRE, and Carba NP colorimetric test, PCR and sequencing for CRE confirmation test. Each method has its own advantages and disadvantages, and can be applied according to the local main popular CRE genetypes and experimental conditions.

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