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Article in Chinese | WPRIM | ID: wpr-879867


OBJECTIVE@#To study the effect of astragaloside IV (AS-IV) on NOD-like receptor protein 3 (NLRP3) inflammasome in neonatal rats with hypoxic-ischemic brain damage (HIBD).@*METHODS@#A total of 24 Sprague-Dawley rats, aged 7 days, were randomly divided into a sham-operation group, an HIBD group, and an AS-IV treatment group, with 8 rats in each group. After 24 hours of modeling, brain tissue was collected for hematoxylin-eosin staining, yo-PRO-1 staining, and EthD-2 immunofluorescent staining in order to observe the cerebral protection effect of AS-IV in vivo. HT22 cells were used to prepare a model of oxygen-glycogen deprivation (OGD), and a concentration gradient (50-400 μmol/L) was established for AS-IV. CCK-8 assay was used to measure the viability of HT22 cells. RT-PCR and Western blot were used to observe the effect of different concentrations of AS-IV on the mRNA and protein expression of NLRP3, gasdermin D (GSDMD), caspase-1, and interleukin-1β (IL-1β).@*RESULTS@#Yo-Pro-1 and EthD-2 staining showed that compared with the sham-operation group, the HIBD group had an increase in pyroptotic cells with a small number of necrotic cells, and the AS-IV group had reductions in both pyroptotic and necrotic cells. Compared with the sham-operation group, the HIBD group had significantly higher protein expression levels of NLRP3, IL-1β, caspase-1, and GSDMD (@*CONCLUSIONS@#AS-IV may alleviate HIBD in neonatal rats by inhibiting the expression of NLRP3, GSDMD, caspase-1, and IL-1β.

Animals , Animals, Newborn , Brain , Hypoxia-Ischemia, Brain/drug therapy , Inflammasomes , NLR Proteins , Rats , Rats, Sprague-Dawley , Saponins , Triterpenes
Article in Chinese | WPRIM | ID: wpr-878808


This study aimed to clarify the microbial diversity, dominant species and the change of community structures in the fermentation of Liushenqu(Massa Medicata Fermentata), and explore the material foundation of its pharmacodynamics effect. On the basis of standardizing the fermentation process, Massa Medicata Fermentata was prepared by screening and optimizing the recipes and the standard formula issued by the Ministry. The community structure and growth process of fungi and bacteria in the samples at five time points(0, 17, 41, 48, 65 h) in the fermentation process of Massa Medicata Fermentata were analyzed by using isolation and culture of eight different media and high-throughput DNA sequencing technology. The results indicated that the samples of the two recipes pre-sented high microbial diversity at the initial fermentation stage, with Aspergillus spp. as the dominant species. As the fermentation process goes forward, Saccharomycopsis fibuligera and Rhizopus oryzae soon became dominant species from 17 h after fermentation commencement point to the fermentation end, while the other species were inhibited at a lower level from 17 h. The species diversity of bacteria in the initial fermentation samples was also high, and Enterobacter was the dominant species. Enterobacter cloacae, Pediococcus pentosaceus and Cronobacter sakazakii became dominant bacterial species 17 h after fermentation commencement, while the species diversity was decreased. Our results will be a scientific basis for promoting the fermentation process of Massa Medicata Fermentata by using pure microbial cultures.

Drugs, Chinese Herbal , Fermentation , Fungi/genetics , Microbiota , Saccharomycopsis
Article in Chinese | WPRIM | ID: wpr-701092


AIM:To study the regulation of human chromosome 1 open reading frame 109(c1orf109)gene by transcription factor Myc-associated zinc-finger protein(MAZ)in vitro.METHODS:In vitro study,electrophoretic mobili-ty shift assay was performed to screen the binding sites of MAZ in the promoter region of c1orf109 gene.The HeLa cells were co-transfected with the enhanced green fluorescent protein reporter vector driven by c1orf109 promoter,and MAZ and transcription factor specificity protein 1(Sp1)expression plasmids.After 24 h,the transcriptional expression of c1orf109 gene in the co-transfected cells was determined by confocal scanning microscopy and flow cytometry.RESULTS:The c1orf109 promoter could bind to MAZ ,and shared the binding sites with Sp 1.MAZ and Sp1 both inhibited the transcrip-tional expression of c1orf109 gene and the inhibition effect of Sp1 was greater than MAZ(P<0.05).CONCLUSION:Both MAZ and Sp1 regulate the expression of c1orf109 gene in physiological and pathological conditions ,and this regulation is redundancy with the same direction.The existence of redundancy transcriptional regulation manner of this gene suggests that precise regulation of c1orf109 gene is vital important for cell biological processing.

Journal of Medical Biomechanics ; (6): E436-E440, 2011.
Article in Chinese | WPRIM | ID: wpr-804141


Objective To study the variation of rupture distance of liquid bridge with different liquid volume, spherical radius and solid-liquid contact angle. Methods The process of stretching and rupture of liquid bridge between a sphere and a plane was simulated by Surface Evolver program. The rupture distance of liquid bridge which depended on the liquid volume, radius of sphere and wetting property of surface was calculated from the simulation. Results The theoretical formula of rupture distance which was related to the liquid volume, spherical radius and contact angle was derived based on the formula given by Lian and Willett, et al. The simulation result was in agreement with the theoretical result when the solid surface was hydrophilic (error <4.3%); when the solid surface was hydrophobic, the error between the simulation and theoretical results showed to be greater with the hydrophobicity enhanced. Conclusions The quasi-static process of stretching and rupture of liquid bridge can be well simulated by Surface Evolver program and the rupture distance can be given accurately from the simulation result. The error between the simulation and theoretical results in rupture distance on the hydrophobic surface appeared significantly increasing, because the formula given by Lian and Willett, et al was derived from data based on the hydrophilic surface.

Article in Chinese | WPRIM | ID: wpr-814008


OBJECTIVE@#To evaluate the clinical effect and prognostic factors of nasopharyngeal carcinoma in 44 children and adolescents.@*METHODS@#From June 1987 to December 2003,44 children and adolescents with nasopharyngeal carcinoma were treated by radiotherapy, and some patients also received chemotherapy. Kaplan-Meier method was used for the survival rate and univariate analysis, and Cox proportional hazard model was used in multivariate analysis.@*RESULTS@#The 3.5 year survival rate was 84.2% and 62.3%.In the univariate analysis, clinical stage, lymph node (N) stage, radiotherapy dose and chemotherapy were significant prognostic factors of survival.In the multivariate analysis, N stage and chemotherapy were the prognostic factors in the survival rate.@*CONCLUSION@#Most nasopharyngeal carcinomas belong to the advanced degree. These patients are sensitive to radiotherapy and chemotherapy. Combined modality therapy can improve the clinical effect of nasopharyngeal carcinoma in children and adolescents.

Adolescent , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Carcinoma, Squamous Cell , Pathology , Radiotherapy , Child , Combined Modality Therapy , Female , Humans , Male , Nasopharyngeal Neoplasms , Pathology , Radiotherapy , Prognosis , Survival Analysis