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1.
Article in Chinese | WPRIM | ID: wpr-928629

ABSTRACT

Tofacitinib is a Janus kinase inhibitor and can block the Janus kinase-signal transducer and activator of transcription signal transduction pathway and reduce the production and release of a variety of cytokines. It has great potential in the treatment of various rheumatic diseases with a rapid onset of action and can reduce corticosteroid dependence and related adverse events. The therapeutic effect of tofacitinib in adult patients has been confirmed, and it has been increasingly used in pediatric patients in recent years. This article reviews the clinical application of tofacitinib in the treatment of pediatric autoimmune diseases.


Subject(s)
Adult , Child , Humans , Janus Kinases/metabolism , Piperidines/therapeutic use , Protein Kinase Inhibitors/therapeutic use , Pyrimidines/therapeutic use , Rheumatic Diseases/drug therapy
2.
International Eye Science ; (12): 880-883, 2022.
Article in Chinese | WPRIM | ID: wpr-923432

ABSTRACT

@#AIM: To investigate the therapeutic effect of conbercept injection combined with Ahmed valve implantation and phacoemulsification in the treatment of neovascular glaucoma(NVG).<p>METHODS: Retrospective analysis. Totally 18 patients(18 eyes)with NVG complicated combined with cataract in our hospital from June 2018 to January 2020. All patients received intraocular injection of conbercept at 3-7d before surgery, Ahmed valve implantation and phacoemulsification were performed after meeting the surgical indications. Best corrected visual acuity(BCVA), intraocular pressure(IOP), resolution of iris neovascularization and postoperative complications were followed up after the surgery, and all the cases were followed up 12mo. <p>RESULTS: Compared with preoperation(51.44±8.18 mmHg), IOP was significant decreased at 1, 7d, 1, 3, 6mo, 1a after surgery(25.94±11.82, 15.39±4.97, 15.94±2.69, 15.33±4.54, 18.89±7.95, 16.27±5.22 mmHg)(<i>P</i><0.05). Last follow up after surgery, BCVA was improved in 12 eyes(67%), unchanged in 5 eyes(28%)and decreased in one eye. At 1a after operation, the complete success rate was 83%(15 eyes), conditional success rate was 11%(2 eyes), one eye of losing control of IOP was failed. <p>CONCLUSION: Conbercept intraocular injection combined with Ahmed valve implantation and phacoemulsification can not only effectively control postoperative IOP, but also improve the visual acuity of the patients as much as possible. It is an effective method for the treatment of NVG with cataract.

3.
Article in Chinese | WPRIM | ID: wpr-888310

ABSTRACT

OBJECTIVE@#To investigate the short-term clinical effect of double channel decompression and bone grafting through the greater trochanter combined with allograft fibula propping in the treatment of osteonecrosis of femoral head (ONFH).@*METHODS@#Twenty two patients (23 hips) with osteonecrosis of the femoral head were included from November 2017 to February 2019. According to Association Research Cirulation Osseous(ARCO) staging, there were 13 hips at stageⅡgroup, aged from 20 to 48 years old with an average of(32.5±8.5)years old;10 hips at stageⅢgroup, aged from 18 to 45 years old with an average of(32.7±8.6) years old. A single approach through the greater trochanterwas used for decompression, bone grafting and fibula support. Harris scoring system was used to evaluate the function of hip joint before and after implantation, and the anteroposterior and lateral X-ray films of hip joint were taken at 3, 6, 12 and 18 months after implantation to observe and analyze the progress of femoral head necrosis and regeneration.@*RESULTS@#All patients were followed up, and the duration ranged from 12 to 18 months with an average of (14.6±2.1) months. Harris score of stageⅡand stageⅢpatients increased from 73.2± 5.5 and 66.5±3.4 to 87.6±8.7(@*CONCLUSION@#The effect of double trochanteric decompression and bone grafting combined with fibular allograft propping in the treatment of early and middle stage avascular necrosis of the femoral head is good, especially suitable for young and middle aged patients with ARCOⅡstage avascular necrosis of the femoral head.


Subject(s)
Adolescent , Adult , Allografts , Bone Transplantation , Decompression , Femur Head , Femur Head Necrosis , Fibula , Follow-Up Studies , Humans , Middle Aged , Treatment Outcome , Young Adult
4.
China Occupational Medicine ; (6): 111-114, 2021.
Article in Chinese | WPRIM | ID: wpr-881982

ABSTRACT

OBJECTIVE: To analyze the hazardous status of respiratory dust in coal mining enterprises. METHODS: A total of 20 coal mining enterprises in a mining group were selected as the research subjects using a cluster random sampling method. Respiratory dust in the workplace was detected, and the incidence of pneumoconiosis was investigated. RESULTS: The dust types in the workplace of these 20 coal mining enterprises were mainly coal dust and silica dust. The posts with serious coal dust hazard were coal mining and tunneling posts. The M(P_0-P_(100)) of the exposure concentration of time weighted average(C_(TWA)) were 2.38(0.19-14.21) and 2.73(0.49-9.57) mg/m~3. The over-standard rate was 49.2% and 50.0%, respectively, that was higher than the post of gangue selection(all P<0.05 or all P<0.017). The results of short time exposure concentration of short term(C_(STE)) and the over-standard rate were higher in the coal mining and tunneling posts than those of gangue selection and boiler room post(all P<0.05 or all P<0.008). The most serious post with silica dust hazard was the tunneling post, and the M(P_0-P_(100))of C_(TWA) was 1.30(0.38-8.50) mg/m~3, and the over-standard rate was 85.7%. The detection results and over-standard rate of C_(STEL) were higher in the coal mining and tunneling posts than that of boiler room post(all P<0.01). The coal dust over-standard rate of C_(TWA) in workplaces of coal mining enterprises increased from 2015 to 2018(P<0.05). However, there was no significant difference in the over-standard rate of silica dust C_(TWA) in different years(P>0.05). From 2015 to 2018, the incidence of pneumoconiosis was 0.3%, 0.3%, 0.2% and 0.3% respectively, and the patients were all from coal mining and tunneling posts. CONCLUSION: The supervision, management and treatment of respiratory dust hazard in coal mining and tunneling posts should be strengthened to protect the health of workers.

5.
Article in Chinese | WPRIM | ID: wpr-879051

ABSTRACT

This project aimed to explore the protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation(H/R)-induced H9 c2 cardiomyocyte injury and its underlying signaling pathway. The H/R model of H9 c2 cardiomyocytes was established and then the cells were divided into different treatment groups. CCK-8(cell counting kit-8) was used to detect the activity of cardiomyocytes; Brdu assay was used to detect the proliferation of H9 c2 cells; the caspase-3 activity was tested, and then the protein expression was assessed by Western blot. Flow cytometry was used to evaluate the apoptosis level of cardiomyocytes. Ginsenoside Rg_1 inhibited H/R-induced cardiomyocyte apoptosis and caspase-3 activity, promoted nuclear transcription of nuclear factor erythroid-2 related factor 2(Nrf2), and enhanced the expression of the downstream heme oxygenase-1(HO-1). Ginsenoside Rg_1 could increase Nrf2 nuclear transcription and HO-1 expression with the increase of concentration(10, 20, 40, 60 μmol·L~(-1)). However, the protective effect of ginsenoside Rg_1 on cardiomyocytes was significantly weakened after the transfection of Nrf2-siRNA. Ginsenoside Rg_1 could protect cardiomyocytes by activating the Nrf2/HO-1 pathway.


Subject(s)
Apoptosis , Ginsenosides/pharmacology , Heme Oxygenase-1/genetics , Humans , Hypoxia , Myocytes, Cardiac , NF-E2-Related Factor 2/genetics
6.
Article in Chinese | WPRIM | ID: wpr-873520

ABSTRACT

@#Objective To investigate the association between physical activity and glucose metabolism during the second trimester of pregnancy. Methods A cross-sectional study was conducted from March 2017 to November 2018. 1 083 pregnant women at 20-28 weeks of gestation in Guangzhou,China were recruited into this study. A standardized questionnaire was used to collect demographics and lifestyles. Physical activity was assessed by the international physical activity questionnaire. The participants underwent an oral glucose tolerance test. Multiple linear regression was used to analyze the association between physical activity and blood glucose. Logistic regression analysis model was used to analyze the association between physical activity and gestational diabetes mellitus(GDM) . Results Only 36. 57% of pregnant women met the recommended level of total physical activity. There was a negative correlation ( β= -0. 104, P= 0. 017) between total physical activity and postprandial 2 h blood glucose. Results from different types of physical activities showed that the recreation,sport,and leisure-time physical activity were negatively associated with postprandial 1 h blood glucose ( β = -0. 114,P = 0. 042) . No significant association was found between either various types of physical activities or sedentary time and GDM. Conclusions Physical activity during mid-pregnancy is inadequate in pregnant women in Guangzhou,China. Higher total physical activity and recreation,sport,and leisure-time physical activity in the second trimester of pregnancy are associated with lower postprandial blood glucose.

7.
Article in Chinese | WPRIM | ID: wpr-873331

ABSTRACT

Objective::To clone p-coumaroyl quinate/shikimate 3' -hydroxylase gene from Lonicera macranthoides, and analyze its bioinformatics and expression patterns with chlorogenic acid content, in order to speculate the functions of LmC3H1 gene from L. macranthoides. Method::The full-length cDNA sequence of LmC3H1 gene was cloned by reverse trascription polymerase chain reaction(RT-PCR) and RACE techniques. The bioinformatics analysis of the gene sequence was carried out by using relevant software.Real-time fluorescence quantification PCR(Real-time PCR) and HPLC were used to determine relative expression of LmC3H1 and content of chlorogenic acid in stems, leaves and flowers of different flowering stages. Result::The LmC3H1 (GenBank: MN177695) gene was cloned, and the open reading frame (ORF) of it was 1 533 bp in length and encoded 510 amino acids. The molecular formula was C2618H4134N718O727S22, the relative molecular mass was 58 005.32, and the isoelectric point was 8.92.It was a hydrophilic protein located in the chloroplast with a transmembrane region LLLIPAVLFLISLVYPLI, and contained a conserved domain CYTOCHROME_P450(433-422 aa) in cytochrome P450.The results of Real-time PCR showed that LmC3H1 was expressed in different degrees in stems, leaves and different flowering stages of L. macranthoides. In the flower development stage, the relative expression of white bud stage was the highest, followed by flower buds and white flowering stage. The ratio of flower to stem and leaf was the highest, and the relative expression of flower was the highest. The HPLC results showed that the content of chlorogenic acid increased from greenish white to golden yellow in flowering stage and golden yellow flowering stage. Among the different organs, the flower had the highest chlorogenic acid, and the stem showed the lowest. Conclusion::The LmC3H1 gene of L. macranthoides is cloned, suggesting that LmC3H1 might be involved in the biosynthesis of L. macranthoides chlorogenic acid. This study provides a basis for further studying the functions of the gene and exploring the biosynthesis and regulation mechanism of L. macranthoides chlorogenic acid, while laying the foundation for the genetic improvement of L. macranthoides.

8.
Article in Chinese | WPRIM | ID: wpr-872728

ABSTRACT

Objective:To investigate the effect of icariin on neuroprotection in cerebral ischemia-reperfusion rats and microglia toll-like receptor 4 (TLR4)/nuclear transcription factor-κB (NF-κB) pathway. Method:In the blank group, blood vessels were only isolated but not ligated and blocked,and the rats were injected intraperitoneally with the same volume of normal saline. After successful modeling, they were randomly divided into model group, butyphthalide group (6 mg·kg-1), and high, medium and low (40,20,10 mg·kg-1)-dose icariin group,and abdominally administered with drugs at 5,12, 24 h after ischemia, respectively. The nerve function scores were detected, 2,3,5-triphenyltetrazole chloride (TTC) staining was used to measure the cerebral infarction rate,immunohistochemical assay was performed to detect the expressions of microglial markers ionized calcium binding adapter molecule 1(Iba1) and TLR4 in the rat brain cortex, Western blot immunoassay was used to detect the expression of NF-κB p65 in the cerebral cortex, and enzyme-linked immunosorbent assay (ELISA) was used to detect interleukin-1α (IL-1α) and tumor necrosis factor-α (TNF-α) content. Result:Compared with the sham-operation group, the nerve score, the cerebral infarction rate, the activations of Iba1 and TLR4 in microglial cells, the protein expression of NF-κB p65(P<0.01), and the contents of inflammatory factors IL-1α and TNF-α in the model group increased significantly(P<0.01). After treatment with icariin, compared with the model group, the neurological function score and the cerebral infarction rate of rats were improved, whereas the activations of Iba1 and TLR4 in microglia, the protein expression of NF-κB p65, and the contents of inflammatory factors IL-1α and TNF-α decreased obviously(P<0.05,P<0.01). Conclusion:Icariin may inhibit the activations of TLR4 and its downstream NF-κB signaling pathway and reduce the expression of relevant inflammatory factors IL-1α and TNF-α by regulating the activation of microglia, so as to play a protective role in the brain after stroke.

9.
Chinese Journal of Radiology ; (12): 417-422, 2020.
Article in Chinese | WPRIM | ID: wpr-868308

ABSTRACT

Objective:To explore the enhancement effect of different concentrations of contrast media on blood vessels and hollow organs in CT enterography.Methods:Sixty patients with CT enterography were enrolled from January to August 2019 at Shanghai Ninth People's Hospital, and were prospectively randomly divided into three groups (group A: 90 ml 400 mg/ml contrast media, group B: 90 ml 350 mg/ml contrast media, and group C: 79 ml 400 mg/ml contrast media). Evaluation parameters included CT value, signal noise ratio (SNR) and contrast noise ratio (CNR) of main abdominal vessels (abdominal aorta, superior and inferior mesenteric atery, jejunal artery, ileum artery, superior and inferior mesenteric vein), jejunum, ileum and inflammatory bowel disease. The overall image quality and direct jejunum ileum artery quality scores were evaluated. One-way ANOVA was used to compare the parameters among the three groups.Results:There was no statistical difference in CT value, SNR and CNR of each measurement index among the three groups in plain scan ( P>0.05). The CT value, SNR and CNR of abdominal aorta, superior mesenteric artery, inferior mesenteric artery, jejunal artery and ileal artery in group A and C were higher than those in group B in arterial phase ( P<0.05), but there was no significant difference between group A and group C ( P>0.05). The CT value, SNR and CNR of superior mesenteric vein, inferior mesenteric vein, normal jejunum, ileum and inflammatory lesions in group A were higher than those in group B and C in venous phase ( P<0.05), but there was no significant difference between group B and group C ( P>0.05). The overall image quality scores of group A, B and C were (3.8±0.7), (3.4±0.6), (3.4±0.6), respectively, with no significant difference ( F=3.075, P=0.054). The direct jejunum ileum artery scores of the three groups were (3.5±0.5), (3.1±0.5), (3.4±0.5), respectively, the difference was statistically significant ( F=3.684, P=0.031). Conclusion:At the same injection rate and scanning parameters, contrast media at 400 mg/ml can provide better vessel and hollow organ enhancement.

10.
Article in Chinese | WPRIM | ID: wpr-862703

ABSTRACT

<b>Objective::To compare the protective effect of different bile (porcine bile, oxgall and sheep bile) and their Arisaema cum Bile on rats with acute lung injury, so as to provide reference for the selection of bile and the classification of decoction pieces of Arisaema cum Bile. <b>Method::Wistar male rats were randomly divided into 8 groups (<italic>n</italic>=12), including blank group, model group, porcine bile group, oxgall group, sheep bile group, Arisaema cum Bile with porcine bile group, Arisaema cum Bile with oxgall group and Arisaema cum Bile with sheep bile group. Rats in each treatment group were given corresponding drug solution by gavage at 2.52 g·kg<sup>-1</sup> every day, and rats in the model group and the blank group were given the same volume of normal saline by gavage every day for a total of 8 days. On the 8<sup>th</sup> day, after 1 h of administration, rats in the model group and each treatment group were intraperitoneally injected lipopolysaccharide (LPS, 2 mg·kg<sup>-1</sup>) to prepare rat lung injury model. Blood and lung tissues were collected from every four rats at 3, 6, 24 h after LPS injection, respectively. Lung coefficient, lung water content and wet weight/dry weight ratio of lung tissue (<italic>W</italic>/<italic>D</italic>) were measured, and the levels of tumor necrosis factor (TNF)-<italic>α</italic>, interleukin (IL)-6 and thromboxane B<sub>2</sub> (TXB<sub>2</sub>) in serum and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the contents of malondialdehyde (MDA) and matrix metalloproteinase-9 (MMP-9) in lung tissue were determined by enzyme linked immunosorbent assay (ELISA). The pathological morphology of rat lung tissue was observed and the score of lung tissue injury was calculated. <b>Result::Compared with the model group at the same time point, the lung coefficient, <italic>W</italic>/<italic>D,</italic> lung water content, contents of TNF-<italic>α</italic>, IL-6 and TXB<sub>2</sub> in serum, contents of MDA and MMP-9 in lung tissue of rats in each treatment group were all decreased, and most of them had significant differences (<italic>P</italic><0.05, <italic>P</italic><0.01), but the activities of GSH-Px and SOD were all increased, and most of them had significant differences (<italic>P</italic><0.05, <italic>P</italic><0.01). The oxgall group and the sheep bile group were superior to the porcine bile group in most of the indexes, the Arisaema cum Bile with oxgall group and the Arisaema cum Bile with sheep bile group were superior to the Arisaema cum Bile with porcine bile group, and some of them had significant differences(<italic>P</italic><0.05, <italic>P</italic><0.01). <b>Conclusion::Each bile group and each Arisaema cum Bile group all have protective effects on rats with acute lung injury induced by LPS, and the therapeutic effect of oxgall and sheep bile is better than that of porcine bile, the therapeutic effect of Arisaema cum Bile prepared by oxgall and sheep bile is better than that of Arisaema cum Bile prepared by porcine bile.

11.
Article in Chinese | WPRIM | ID: wpr-905500

ABSTRACT

Spinal cord stimulation (SCS) can inhibit or alleviate pain by blocking pain signal transmission, interfering with pain pathway, activating opioid pathway, stimulating locus coeruleus system and regulating γ-aminobutyric acid ergic. It is applied in the treatment of neuropathic pain (NP), such as post-herpetic neuralgia, failed back surgery syndrome, phantom limb pain, painful diabetic neuropathy, head and facial neuropathic pain, pudendal neuralgia, etc.

12.
Article in English | WPRIM | ID: wpr-827456

ABSTRACT

OBJECTIVE@#To evaluate the clinical efficacy and safety of Congrong Shujing Granules ( , CSGs) in treating patients with Parkinson's disease (PD) and Chinese medicine (CM) syndrome of Shen (Kidney) essence deficiency, and to investigate the potential mechanism involving efficacy through a transcriptome sequencing approach.@*METHODS@#Eligible PD patients with syndrome of Shen essence defificiency were randomly assigned to a treatment group or a control group by a random number table, and were treated with CSGs combined with Western medicine (WM), or placebo combined with WM, respectively. Both courses of treatment lasted for 12 weeks. The Unifified Parkinson's Disease Rating Scale (UPDRS) score, the PD Question-39 (PDQ-39) score, CM Syndrome Scale score, and drug usage of all patients were evaluated before and after treatment. Safety was evaluated by clinical laboratory tests and electrocardiographs. Blood samples from 6 patients in each group were collected before and after the trial and used for transcriptomic analysis by gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis. Differentially expressed genes were validated using reverse transcription-polymerase chain reaction.@*RESULTS@#A total of 86 PD patients were selected from the Third Affifiliated People's Hospital of Fujian University of Traditional Chinese Medicine between January 2017 and December 2017. Finally, 72 patients completed the trial, including 35 in the treatment group and 37 in the control group. When compared with the control group after treatment, patients in the treatment group showed signifificant decreases in UPDRS sub-II score, PDQ-39 score, CM syndrome score, and Levodopa equivalent dose (P0.05). A possible mechanism of clinical effificacy was proposed that involved regulating cell metabolism-related processes and ribosome-related pathways. Treatment with CSGs had shown to affect relevant gene loci for PD, including AIDA, ANKRD36BP2, BCL2A1, BCL2L11, FTH1P2, GCH1, HPRT1, NFE2L2, RMRP, RPS7, TGFBR1, WIPF2, and COX7B.@*CONCLUSIONS@#CSGs combined with WM can be used to treat PD patients with CM syndrome of Shen essence defificiency with a good safety. The possible mechanism of action and relevant gene loci were proposed. (Registration No. ChiCTR-IOR-16008394).

13.
Chinese Journal of Pathology ; (12): 626-632, 2019.
Article in Chinese | WPRIM | ID: wpr-805766

ABSTRACT

Objective@#To investigate the expression and significance of MNAT1 in non-small cell lung cancer (NSCLC) and to explore the biological impact of MNAT1 expression in lung cancer cells at the cellular level and related signaling pathway.@*Methods@#Forty-eight cases of NSCLC tissues and paired normal tissues was collected at Nanfang Hospital, Southern Medical University from 2015 to 2017. The expression level of MNAT1 was detected by immunohistochemistry, and the relationship between MNAT1 and clinicopathological features was analyzed. The expression of MNAT1 was detected in lung cancer cells, MNAT1 level was analyzed after knocking down in A549 and H322 cells by siRNA; Plasmid vector of overexpressing MNAT1 was constructed, followed by transfecting H1299 cells and observing proliferation and migration at the cellular level. Flow cytometry was used to analyze the effect of the expression of MNAT1 on cell cycle, and Western blot was used to explore the possible molecular mechanism of MNAT1 on cell proliferation and cell cycle.@*Results@#Immunohistochemistry showed that the expression score of MNAT1 was (4.07±3.55) in normal lung tissue and (7.33±4.09) in NSCLC tissue (P<0.01), and correlated with lymph node metastasis. At the cellular level, MNAT1 promoted cell proliferation(P<0.05), migration(P<0.05) and cell cycle progression(P<0.01).@*Conclusions@#MNAT1 may be involved in the development of non-small cell lung cancer.MNAT1 affects cell cycle and proliferation through the Akt/p21 pathway.

14.
Chinese Journal of Neurology ; (12): 606-611, 2018.
Article in Chinese | WPRIM | ID: wpr-710992

ABSTRACT

Objective To assess the cerebrovascular reserve (CVR)and the relationship of CVR with the short-term prognosis in patients with acute atherosclerotic cerebral infarction.Methods A total of 106 patients with unilateral acute (within 72 h) atherosclerotic cerebral infarction (trial group) were selected from December 2016 to December 2017 in the Department of Neurology of the First People's Hospital of Hefei,which were divided into two groups including the lesion group (106 cases) and the nonlesion group (106 cases).The median score of NIHSS in patients was 4(2,5).The control group included 40 healthy controls.The cerebral blood flow reserve and pulsatility index were measured by transcranial Doppler combined with CO2 inhalation test in both the trial group and the control group.According to the rate of change of cerebral blood flow velocity (CBFV),all subjects were divided into two groups including the normal group and the impaired cerebral blood flow reserve group.The changes of CBFV were compared in the control group and the trial group,which was divided into two groups including the group with lesion side and the group with non-lesion side.To evaluate the brain structure reserve the circle of Willis in the trial group was assessed by MRA.According to the integrity of the circle of Willis anterior and posterior circulation all subjects were divided into four groups (type Ⅰ,type Ⅱ,type Ⅲ and type Ⅳ).The effect of the factors,such as diabetes,hypertension,low density lipoprotein (LDL),high density lipoprotein (HDL),smoking,and drinking history,on cerebral blood flow reserve was measured by single-factor analysis.The correlation of NIHSS scores,infarct size and volume with CVR was also measured.All patients in the trial group were treated with drugs and were followed-up for three months.The modified Rankin Scale (Mrs) was used to evaluate the prognosis of the patients.It means poor prognosis if the value of Mrs was more than three.The effects of factors,such as sex,HDL,LDL,diabetes,hypertension,smoking history,drinking history,cerebral blood flow reserve,NIHSS scores,brain structure reserve,infarct location,age,on the prognosis were measured by multivariate Logistic regression.Results The increase rate of CBFV in the lesion-side of patients with atherosclerotic cerebral infarction was 5.94% (2.18%,10.49%),and the increase rate of pulsatility index was 10.77% (2.21%,22.62%),which were both lower than the control group (CBFV:11.54% (5.01%,17.96%),Z =2.547,P<0.05);pulsatility index:48.36% (33.93%,64.51%),Z =6.604,P < 0.01).There was significant difference (x2 =4.328,P < 0.05) in the distribution of diabetes,which was 2/14 in the normal group and 43.48% (40/92) in the impaired cerebral blood flow reserve group.And in the trial group the brain structural reserve was positively correlated to the infarct volume and the NIHSS score,and the rank correlation coefficient was 0.219 and 0.238 respectively (P < 0.05).The prognosis of cerebral blood flow reserve in the normal group was better than the impaired group (x2 =4.155,P < 0.05),for example,the proportion of patients with good prognosis and normal CBFV was 18.84% (13/69),the proportion of patients with good prognosis but decreased CBFV was 81.16% (56/69),the proportion of patients with poor prognosis but normal CBFV was 2.70% (1/37),the proportion of patients with poor prognosis and decreased CBFV was 97.30% (36/37).The proportion of patients with type Ⅰ and type Ⅲ of the brain structure reserve was 37.68% (26/69) and 5.80% (4/69) respectively,whose prognosis was better (x2 =8.456,P < 0.05) than patients with type Ⅱ and type Ⅳ,whose proportion was 43.48% (30/69) and 13.04% (9/69).Multivariate Logistic regression analysis showed that NIHSS score,age,and brain structural reserve were risk factors for poor prognosis in the trial group.Normal cerebral blood flow reserve was a protective factor for good prognosis.Conclusions CVR in patients with acute atherosclerotic cerebral infarction is significantly reduced.CVR can be used as an index to evaluate the prognosis of patients who were followed-up for three months.

15.
Article in Chinese | WPRIM | ID: wpr-708469

ABSTRACT

Objective To investigate the impact of combined postoperative transcatheter arterial chemoembolization (TACE) with antiviral therapy in hepatitis B-related hepatocellular carcinoma (HBV-HCC) patients with high risks of recurrence.Methods Fifty-three consecutive patients who underwent curative resection of HBV-HCC between January 2014 to February 2016 were enrolled.These patients were assigned to either the adjuvant antiviral therapy combined with TACE group (n =32),the treatment group or the no adjuvant treatment group (n =21,the control group).The recurrence-free survival (RFS) and overall survival (OS) were analyzed.Results There was no significant difference between the two groups in clinical characteristics (P>0.05).The recurrence-free survival (RFS) (mean±S.D.) was (20.1 ± 1.8) months in the treatment group and (18.7±2.4) months in the control group (P=0.752).The 1-,2-and 3-year RFS rates of the treatment group and the control group were 65.6% vs.57.1%,31.3% vs.28.6% and 15.6% vs.14.3%,respectively (P>0.05).The overall survival (OS) (mean±S.D.) was (26.8± 1.7) months in the treatment group and (21.1±2.2) months in the control group (P=0.037).The 1-,2-and 3-year RFS rates were 65.6% vs.57.1%,31.3% vs.28.6% and 15.6% vs.14.3% in the treatment group and the control group,respectively.The 1-,2-,and 3-year OS rates were 87.5% vs.66.7%,59.4% vs.38.1% and 43.8% vs.19.0% in the treatment group and the control group,respectively.Conclusion Antiviral therapy in combination with TACE did not decrease the RFS rate,but it improved the OS rate in HCC patients with high risks of recurrence.

16.
Article in Chinese | WPRIM | ID: wpr-708413

ABSTRACT

Objective To study the effectiveness and safety of prophylactic anticoagulation in the prevention of portal venous thrombosis (PVST) in patients after laparoscopic splenectomy.Methods A systematic search of the PubMed,Embase,Cochrane Library,Sinomed,Wangfang,Weipu and CNKI databases was performed to identify studies which compared outcomes in patients with or without prophylactic anticoagulation after laparoscopic splenectomy.The quality of the included studies was assessed using the Cochrane collaboration tool and the Newcastle-Ottawa Scale.Heterogeneity was evaluated using the x2 and I2 tests.The primary outcome was the incidence of postoperative PVST.Results Five studies were included into this review,which involved 206 and 168 patients with or without prophylactic anticoagulation,respectively.The incidence of PVST was significantly reduced with prophylactic anticoagulation with an odds ratio (OR) of 0.32 [95% confidence interval (CI),0.13 ~0.79,P<0.05].Conclusion Prophylactic anticoagulation resulted in a significant reduced incidence of PVST after laparoscopic splenectomy.

17.
Article in Chinese | WPRIM | ID: wpr-704320

ABSTRACT

In order to search for new adefovir analogues as anti-HBV agents with enhanced antiviral activity and hepatotrophic property,adefovir bis L-amino acid ester was used as lead compound to produce ten adefovir mono L-(thio)amino acid ester, mono bile acid ester derivatives(6a-6j). The design based on bile acid prodrug strategy,which can improve drug oral bioavaliability and liver-targeted enrichment by using enterohepatic circula-tion of bile acid.Sub-structure combination method was adopted to introduce L-(thio)amino acid ester and bile acid ester fragments on the phosphonate functionality of adefovir. The structures of target compounds were confirmed by 1H NMR, 13C NMR,ESI-MS and ESI-HRMS.HepG 2.2.15 cell were used for in vitro anti-HBV activity assessment.Compound 6c with high antiviral activity(EC500.92μmol/L,SI 512.63)was further investi-gated for its tissue distribution in mice.The results showed that content of compound 6c in liver was higher than that of adefovir dipivoxil,and in contrast its content in kidney was lower than that in positive control at all time points(0.25-12 h).Compound 6c exhibits higher antiviral activity,selective index and higher liver distribution,making it a potential anti HBV agent for further investigation.

18.
Journal of Medical Postgraduates ; (12): 294-299, 2018.
Article in Chinese | WPRIM | ID: wpr-700821

ABSTRACT

Objective The invasive colonoscopy is an important method for evaluating the severity of intestine and the muco-sal healing in ulcerative colitis(UC)patients. The aim of this study was to investigate the advantage of fecal calprotectin(FC)in diagnosing UC endoscopic activity and severity and analyze the FC correlation with endoscopic scores. Methods A total of 70 UC in-patients in our hospital from January 2016 to September 2017 were retrospectively analyzed and categorized into endoscopic remission UC [mucosal healing(n=12),mucosal lesion(n=10)]and endoscopic active UC[mild-moderate(n=30)and severe(n=18)]according to UC Endoscopic Index of Severity(UCEIS).The ROC curve was used to analyze the predictive efficacy of each laboratory indicator to identify endoscopically active UC and severe UC and evaluate the correlation of UCEIS with all these indicators. Results In UC pa-tients,the levels of FC[828.5(416.6,1079.7)μg/g],PCT[0.03(0.02,0.06)μg/L]and IL-6[13.4(7.32,21.45)ng/L]were sig-nificantly higher in endoscopically active UC than those[43.4(9.4,91.4)μg/g、0.02(0.02,0.03)μg/L、5.82(5.14,6.73)ng/L]in remission and the levels of CRP,ESR,WBC count and PLT count also increased significantly,while HB and ALB levels were signifi-cantly lower than those in remission(all P value<0.001).In endoscopically active UC patients,the levels of FC[1117.1(916.5,1492. 4)μg/g]and IL-6[18.18(12.72,33.25)ng/L]were significantly higher than those of mild-moderate UC[622.4(218.7,924.2)μg/g,8.27(7.08,16.60)ng/L](P<0.05)and the levels of CRP,ESR and WBC also increased significantly(P<0.05),while the ALB showed a significant lower level(P=0.002). When FC>175.6 μg/g,it was of higher accuracy in diagnosing UC endoscopic activity and the sensitivity,specificity,positive predictive value and negative predictive value were respectively 87.50%,90.91%,95.45%and 76.92%;When FC>781.1 μg/g,it was of higher accuracy in diagnosing severe UC and the sensitivity,specificity,positive predic-tive value and negative predictive value were respectively 88.89%,73.33%,66.66% and 91.66%. The levels of FC,PCT,IL-6, CRP,ESR,WBC and PLT all presented positive correlation with UCEIS(P<0.001),while the HB and ALB levels showed a negative correlation with UCEIS(P<0.001). Conclusion FC is a good indicator to judge UC endoscopic activity and severe UC. FC can be used as a non-invasive and surrogate marker for endoscopy to monitor UC disease activity in clinical practice.

19.
Article in Chinese | WPRIM | ID: wpr-700233

ABSTRACT

Objective To investigate the relationship between the results of preoperative needle electromyography and the recovery of abductor pollicis brevis (APB) function in patients with severe carpal tunnel syndrome. Methods The clinical data of 68 patients (76 hands) with severe carpal tunnel syndrome were retrospectively analyzed. According to the results of preoperative needle electromyography, the patients were divided into the motor unit potential (MUP) positive group (46 cases, 52 hands), and MUP negative group (22 cases, 24 hands). The patients were followed up 1 year after operation, and the manual muscle testing (MMT) grade of abductor pollicis brevis muscle, functional assessment of thumb and patient satisfaction was recorded. Results The MMT grade ≥ 3 rate and satisfaction rate 1 year after operation in MUP positive group were significantly higher than those in MUP negative group: 100.0% (52/52) vs. 75.0% (18/24) and 100.0% (52/52) vs. 66.7% (16/24), and there were statistical differences (P<0.05). The patient′s thumb function 1 year after operation was evaluated by coin pickup test, button fastening test and sewing needle clamp test. The results showed that the thumb function in MUP positive group was significantly better than that in MUP negative group, and there was statistical difference (P<0.05). Conclusions The recovery of postoperative thumb function is related with the evocation MUP of postoperative abductor pollicis brevis needle electromyography. For MUP positive patients, there is no need for secondary thumb opponensplasty 1 year after operation. Needle electromyography has a certain reference value for thumb opponensplasty.

20.
Article in Chinese | WPRIM | ID: wpr-698457

ABSTRACT

BACKGROUND: As an emerging cell therapy, stem cell therapy has gradually attracted the attention of researchers in the field of medicine. The mechanisms of stem cell therapy mainly include differentiation and paracrine mechanisms. In recent years, with the further research, people gradually put attention to paracrine mechanisms, especially after the discovery of the great protective potential of stem cell-derived exosomes which can provide a powerful repair tool for the "acellular" treatment of regenerative medicine. OBJECTIVE: To review the repair, protection and possible biological mechanisms of stem cell-derived exosomes in various disease damage models. METHODS: The literature search was performed in PubMed, CNKI and WanFang databases, and the keywords were "stem cell-derived exosomes, exosome, regenerative medicine" in English and Chinese, respectively. According to the inclusion and exclusion criteria, 41 articles were finally reviewed. RESULTS AND CONCLUSION: Stem cell-derived exosomes show outstanding ability to repair and protect many diseases, such as cardiovascular system, traumatic brain injury, musculoskeletal system, liver damage, renal injury, and so on. Although most studies have yet to report their detailed molecular mechanisms of action, stem cell-derived exosomes are expected to become a new "acellular" approach for the repair and protection of organism damage.

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