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1.
Journal of Experimental Hematology ; (6): 1617-1623, 2023.
Article in Chinese | WPRIM | ID: wpr-1010013

ABSTRACT

OBJECTIVE@#To investigate the effect of miR-22 targeting formin-like protein 2 (FMNL2) on the migration and apoptosis of childhood acute myeloid leukemia (AML) cells.@*METHOD@#Peripheral blood samples from 11 children with AML, 10 children with immune thrombocytopenia, human AML cell lines TF-1a, HL-60, THP-1 and human bone marrow stromal cells HS-5 were used as the research objects. UniCel DxH 800 automatic hematology analyzer detected platelet count, hemoglobin, and white blood cell count in peripheral blood samples, and RT-qPCR detected miR-22 expression in peripheral blood samples and AML cells. HL-60 cells were transfected with LipofectamineTM 2000 kit, the experiments were divided into seven groups: blank (no cells transfected), miR-NC, miR-22 mimics, si-NC, si-FMNL2 , miR-22 mimics+OE-NC and miR-22 mimics+OE-FMNL2 . RT-qPCR was used to detect the expression of miR-22 in each group. Transwell was used to detect cell migration. Flow cytometry was used to detect cell apoptosis. Dual-luciferase reporter gene detection experiments verified the targeting relationship between miR-22 and FMNL2 . Western blot was used to detect the expression of FMNL2 protein.@*RESULTS@#Compared with the control group, the number of leukocytes in the peripheral blood of children with AML was significantly increased (P <0.001), while the concentration of hemoglobin and the number of platelets were significantly decreased P <0.001). The expression level of miR-22 in peripheral blood of children with AML was significantly lower than that in control group (P <0.001). Compared with HS-5 cells, the expression levels of miR-22 in TF-1a, HL-60, and THP-1 cells were significantly decreased (P <0.05), and in HL-60 cells was the lowest. Therefore, HL-60 cells were selected for subsequent experiments. Up-regulation of miR-22 or silencing of FMNL2 could reduce the number of migrating cells and increase apoptosis rate (P <0.05). MiR-22 targeted and negatively regulated the expression of FMNL2 . FMNL2 overexpression reversed the effects of up-regulated miR-22 on migration and apoptosis of HL-60 cells.@*CONCLUSION@#MiR-22 can inhibit the migration and promote apoptosis of HL-60 cells by down regulating the expression of FMNL2 .


Subject(s)
Humans , Child , MicroRNAs/metabolism , Leukemia, Myeloid, Acute/metabolism , Cell Proliferation , Apoptosis , Myeloproliferative Disorders , Cell Movement , Hemoglobins , Cell Line, Tumor , Formins
2.
Chinese Journal of Contemporary Pediatrics ; (12): 33-40, 2022.
Article in English | WPRIM | ID: wpr-928563

ABSTRACT

OBJECTIVES@#To investigate the clinical treatment outcomes and the changes of the outcomes over time in extremely preterm twins in Guangdong Province, China.@*METHODS@#A retrospective analysis was performed for 269 pairs of extremely preterm twins with a gestational age of <28 weeks who were admitted to the department of neonatology in 26 grade A tertiary hospitals in Guangdong Province from January 2008 to December 2017. According to the admission time, they were divided into two groups: 2008-2012 and 2013-2017. Besides, each pair of twins was divided into the heavier infant and the lighter infant subgroups according to birth weight. The perinatal data of mothers and hospitalization data of neonates were collected. The survival rate of twins and the incidence rate of complications were compared between the 2008-2012 and 2013-2017 groups.@*RESULTS@#Compared with the 2008-2012 group, the 2013-2017 group (both the heavier infant and lighter infant subgroups) had lower incidence rates of severe asphyxia and smaller head circumference at birth (P<0.05). The mortality rates of both of the twins, the heavier infant of the twins, and the lighter infant of the twins were lower in the 2013-2017 group compared with the 2008-2012 group (P<0.05). Compared with the 2008-2012 group, the 2013-2017 group (both the heavier infant and lighter infant subgroups) had lower incidence rates of pulmonary hemorrhage, patent ductus arteriosus (PDA), periventricular-intraventricular hemorrhage (P-IVH), and neonatal respiratory distress syndrome (NRDS) and a higher incidence rate of bronchopulmonary dysplasia (P<0.05).@*CONCLUSIONS@#There is a significant increase in the survival rate over time in extremely preterm twins with a gestational age of <28 weeks in the 26 grade A tertiary hospitals in Guangdong Province. The incidences of severe asphyxia, pulmonary hemorrhage, PDA, P-IVH, and NRDS decrease in both the heavier and lighter infants of the twins, but the incidence of bronchopulmonary dysplasia increases. With the improvement of diagnosis and treatment, the multidisciplinary collaboration between different fields of fetal medicine including prenatal diagnosis, obstetrics, and neonatology is needed in the future to jointly develop management strategies for twin pregnancy.


Subject(s)
Female , Humans , Infant , Infant, Newborn , Pregnancy , Bronchopulmonary Dysplasia/epidemiology , Gestational Age , Infant, Extremely Premature , Respiratory Distress Syndrome, Newborn/epidemiology , Retrospective Studies , Treatment Outcome
3.
Chinese Medical Journal ; (24): 1276-1285, 2021.
Article in English | WPRIM | ID: wpr-878166

ABSTRACT

Excessive consumption of fructose, the sweetest of all naturally occurring carbohydrates, has been linked to worldwide epidemics of metabolic diseases in humans, and it is considered an independent risk factor for cardiovascular diseases. We provide an overview about the features of fructose metabolism, as well as potential mechanisms by which excessive fructose intake is associated with the pathogenesis of metabolic diseases both in humans and rodents. To accomplish this aim, we focus on illuminating the cellular and molecular mechanisms of fructose metabolism as well as its signaling effects on metabolic and cardiovascular homeostasis in health and disease, highlighting the role of carbohydrate-responsive element-binding protein in regulating fructose metabolism.


Subject(s)
Humans , Fructose/adverse effects , Homeostasis , Metabolic Diseases/etiology
4.
Biomedical and Environmental Sciences ; (12): 693-704, 2021.
Article in English | WPRIM | ID: wpr-921320

ABSTRACT

Objective@#The aim of this study was to explore the effects of 2-hexyl-4-pentylenic acid (HPTA) in combination with radiotherapy (RT) on distant unirradiated breast tumors.@*Methods@#Using a rat model of chemical carcinogen (7,12-dimethylbenz[a]anthracene,DMBA)-induced breast cancer, tumor volume was monitored and treatment response was evaluated by performing HE staining, immunohistochemistry, immunofluorescence, qRT-PCR, and western blot analyses.@*Results@#The results demonstrated that HPTA in combination with RT significantly delayed the growth of distant, unirradiated breast tumors. The mechanism of action included tumor-associated macrophage (TAM) infiltration into distant tumor tissues, M1 polarization, and inhibition of tumor angiogenesis by IFN-γ.@*Conclusion@#The results suggest that the combination of HPTA with RT has an abscopal effect on distant tumors


Subject(s)
Animals , Female , Rats , Antineoplastic Agents/therapeutic use , Cell Proliferation/radiation effects , Combined Modality Therapy , Cytokines/immunology , Fatty Acids, Unsaturated/therapeutic use , Mammary Neoplasms, Experimental/radiotherapy , Tumor-Associated Macrophages/radiation effects
5.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 215-221, 2019.
Article in Chinese | WPRIM | ID: wpr-801787

ABSTRACT

Diabetes is a lifelong non-communicable disease caused by continuous high blood sugar,which poses a great threat to people's health. Diabetic cognitive dysfunction is the neurological impairment caused by the disorder of glucose and lipid metabolism in diabetes mellitus,which is characterized by inattention,decreased learning and memory ability,and then developed into alzheimer's disease.The pathogenesis of diabetic cognitive dysfunction is not fully understood.there are no effective drugs for this disease, hypoglycemic drugs are mainly used in the clinical treatment of diabetic cognitive dysfunction,and most drugs are accompanied by serious adverse reactions while playing a therapeutic role,drug resistance and liver injury are easy to occur.In recent years,there have been a lot of research achievements on the prevention and treatment of cognitive dysfunction in diabetes mellitus with traditional Chinese medicine(TCM).Modern medicine believes that diabetes belongs to the category of consumptive thirst of TCM, and the diabetic cognitive dysfunction is consumptive thirst combined with dementia and forgetfulness.Qi and Yin deficiency runs through the whole process of consumptive thirst,the Qi injured,Then the Qi defficiency would cause blood circulation malfunction, long-time poor blood condition would cause blood stagnating in the brain and blocking the brain,which would lead to the brain disease.TCM can improve the diabetic cognitive dysfunction by lowering blood sugar,inhibiting neuron damage, deposition of Amyloid beta(Aβ)and abnormal phosphorylation of Tau protein.This article reviews the pathogenesis of diabetic cognitive dysfunction from glucolipid metabolic disorders,disruption of blood brain barrier,inflammation,oxidative stress and non enzymatic glycosylation,insulin resistance in diabetes etc.,and explores the prevention and treatment of diabetic cognitive dysfunction by Chinese medicine,to provide a reference for the research and development of drug prevention and treatment of diabetic cognitive dysfunction.

6.
Chinese Journal of Practical Surgery ; (12): 1037-1040, 2019.
Article in Chinese | WPRIM | ID: wpr-816503

ABSTRACT

The low resectability rate of recurrent hepatocellular carcinoma,abdominal adhesion and anatomical variation caused by the original hepatectomy increase the difficulty and risk of liver re-resection,which hampers the development of minimally invasive management of posthepatectomy hepatocellular carcinoma recurrence. In recent years,with the update of laparoscopic equipment and the accumulation of surgeon experience, preliminary exploration of laparoscopic liver re-resection for patients with posthepatectomy hepatocellular carcinoma recurrence has begun. In patients with strict indications,laparoscopic reresection can significantly reduce the incidence of perioperative complications and achieve similar survival results as the open surgery. However,as the treatment is still in the exploratory stage,it is recommended to perform only under the strict case selection of medical centers with rich experience in open and laparoscopic liver resection. High quality clinical research is still needed to promote its standardization.

7.
Chinese Journal of Biochemical Pharmaceutics ; (6): 43-45, 2015.
Article in Chinese | WPRIM | ID: wpr-485065

ABSTRACT

Objective To investigate the effect of controlled-release dinoprostone on odinopoeia in prolonged pregnancy.Methods 94 cases of prolonged pregnancy women selected in First People's Hospital in Pinghu City from January 2012 to November 2014 were randomly divided into two groups, 47 cases in each group.The control group were given misoprostol to induce labour, while the observation group were given controlled-release dinoprostone to induce labour.The Bishop score, odinopoeia effect and adverse reactions were compared between two groups and expressions of prostaglandin E2(PGE2) and PGF2αin deciduas of observation group were compared between induction success group and induction failure group. Results The Bishop score in observation group was higher than that in control group 12h post-administration(P<0.05).The total efficiency of cervical ripening, the induction success rate in observation group were higher than those in control group(P<0.05).The adverse reactions and the incidence of complications between two group was not statistically significant.The PGE2 and PGF2αconcentrations in induction success group were higher than those in induction failure group(P<0.05).Conclusion The controlled-release dinoprostone could achieve similar security of misoprostol in odinopoeia, but has more significant induction effect.

8.
Chinese Journal of Ultrasonography ; (12): 242-245, 2015.
Article in Chinese | WPRIM | ID: wpr-474940

ABSTRACT

Objective To set up an objective criterion for BI-RADS assessment.Methods The breast sonograms of 1 938 female cases were retrospectively studied which had been confirmed by pathology from January to September 2011 and 2012 January.1 660 cases during 2011 were model cases,and the importance of each single feature in distinguishing between benign and malignant was obtained with Chi square and OR value.A detailed criterion of BI-RADS assessment category was set up based on US-BI-RADS.Results Eighteen of 24 lexicons were statistically significant distinguished between benign and malignant breast masses(P < 0.05).The lexicons were divided into major suspicious signs,middle ones and minor ones assigned 3 points,2 points and 1 point,and a scoring model was established as follows:Score =3 * (X1 + X2 + … + X6) + 2 * (X7 + X8 + … + X12) + (X9 + X10 + … + X18).Based on BI-RADS,the positive predictive value of model cases was 1.5%,6.9%,22.1%,62.5%,96.1% followed by category 3,4A,4B,4C and 5,and it was 1.4%,3.4%,21.1%,69.4%,92.7% in test cases.Conclusions The scoring model could be useful for BI-RADS final assessment more objectively,and could make it more convenient to predict the risk of breast cancer.

9.
Acta Pharmaceutica Sinica ; (12): 1637-1644, 2015.
Article in Chinese | WPRIM | ID: wpr-320030

ABSTRACT

This study was designed to use iTRAQ technology coupled with 2D LC-MS/MS to study the comparative proteomics of different processing technology for pilose antler. 1015 proteins were identified with 2D LC combined with MOLDI TOF/TOF mass spectrometry. Comparative analysis with Protein Pilot (Version 4.5) revealed that 87 proteins were changed (P ≤ 0.05, the ratio of > 1.50 or < 0.60 as the threshold selection of difference proteins), of which 24 were up regulated and 33 were down regulated in the traditional frying process (TFP) compared with the fresh pilose antler (P ≤ 0.05). 7 significant different proteins (P ≤ 0.001), most of these significantly changed proteins were found to be involved in calcium ion binding and ATP binding associated with human healthy. Freeze drying with protective agent (FDP) (Trehalose) can improve the content of significantly different proteins (P ≤ 0.001) including Collagen alpha-1 (XII) chain (COL12A1) and Collagen alpha-1 (II) chain (COL2A1). The significant function involves in platelets activating, maintenance of spermatogonium, and disorder expression in tumor cells. The functional annotation by Hierarchical clustering and GO (gene ontology) showed that the main molecule functions of the proteins significantly changed in these processes were involved in binding (52.7%), catalytic (25.3%), structural molecule and transporter (6.6%).


Subject(s)
Animals , Antlers , Chemistry , Chromatography, Liquid , Collagen , Chemistry , Down-Regulation , Freeze Drying , Gene Expression Regulation , Proteomics , Tandem Mass Spectrometry , Technology, Pharmaceutical , Methods , Up-Regulation
10.
Chinese Journal of Applied Physiology ; (6): 289-293, 2014.
Article in Chinese | WPRIM | ID: wpr-236324

ABSTRACT

<p><b>OBJECTIVE</b>The aim of the present study is to explore the effects of exhaustive exercise-induced oxidative stress on the antioxidant capacity and diformability of rat red blood cells.</p><p><b>METHODS</b>Rats were divided into three group (n = 10): sedentary control (C), exhaustive running exercise (ERE) and moderate running exercise (MRE) groups. Animals in the ERE group started treadmill running at a speed of 20 m/min speed with a 5% gradient, and reached a speed of 25 m/min with gradient 15% in 20 min. Running was continued until exhaustion. MRE group rats running at a speed of 20 m/min with a 5% gradient for 40 min. The levels of free thiol in erythrocyte membrane protein, lipidperoxidation levels and membrane protein components were analyzed. The red blood cell deformability of different groups was also observed.</p><p><b>RESULTS</b>The results showed that red blood cells were damaged by severe oxidative stress and the anti-oxidative capacity decreased significantly under exhaustive exercise conditions. Besides, lipid peroxidation and protein sulfhydryl cross-link based clustering of membrane were found after exhaustive exercise, and polymers high molecular weight (HMW) was formed. The elongation index (EI) was found to decline significantly in the ERE group compared with the C and MRE groups under shear stress (control group, 0.41 +/- 0.01 at 3 Pa and 0.571 +/- 0.008 at 30 Pa; ERE group, 0.314 +/- 0.013 at 3 Pa and 0.534 +/- 0.009 at 30 Pa; P < 0.05 and P < 0.01, respectively).</p><p><b>CONCLUSION</b>These exercise-induced oxidative injure result in a significant decrease in deformability of rat erythrocytes, which in turn leads to dysfunction in the microcirculatory.</p>


Subject(s)
Animals , Male , Rats , Disease Models, Animal , Erythrocyte Deformability , Fatigue , Metabolism , Oxidative Stress , Physical Conditioning, Animal , Rats, Sprague-Dawley
11.
Chinese Journal of Epidemiology ; (12): 1176-1180, 2012.
Article in Chinese | WPRIM | ID: wpr-289557

ABSTRACT

Objective To analyze and summarize the clinical characteristics and risk factors for patients with hemorrhagic transformation (HT) after cerebral infarction to provide guidance for its clinical treatment and prevention.Methods In this study,data from 49 hospitalized patients with HT in the First Department of Neurology,China-Japan Union Hospital of Jilin University from October 2009 to March 2012,were reviewed retrospectively and 106 cases with acute cerebral infarction only during the same period,were chosen randomly as controls.Gender and age of the patients were comparable.Relevant information was collected.SPSS 17.0 statistical package was applied for data processing.Qualitative data were processed with x2 test,and measurable data was processed with t-test.Each index was analyzed with uni-variate analysis while statistically significant risk factors were included in the logistic review model to conduct the multivariate regression analysis.Results (1) Clinical symptoms deteriorating after hemorrhage in 21 cases accounted for 42.9% of the HT group,among which the cases on degree of disturbance to consciousness had an aggravation in 8 cases and hemiplegia increase in another 7 cases.Headaches and dizziness were found in 5 cases.(2)Number of infarction within 15 days after the occurrence of HT accounted for 87.0%.(3)HT-related factors increased the risk of HT in cerebral infarction such as cortical infarction,large area of infarction,atrial fibrillation,cerebral embolism,diabetes and high level of low-density lipoprotein cholesterol (P<0.05).The most important factors were atrial fibrillation and cerebral embolism.(4)PH-2 seemed more unlikely to link with clinical symptoms than other subtypes of HT.Conclusion Cerebral infarction after occlusion of the main artery trunk was prone to HT,especially when it appeared within 15 days.Short-term prognosis seemed to be related to the subtypes of HT,with risk factors as cortical infarct,massive cerebral infarction,atrial fibrillation,cerebral embolism,diabetes,high low-density lipoprotein cholesterol etc.on HT.

12.
Chinese Journal of Surgery ; (12): 83-85, 2009.
Article in Chinese | WPRIM | ID: wpr-238953

ABSTRACT

<p><b>OBJECTIVE</b>To study the therapeutic effect of splenic autotransplantation combined with lower esophagus transaction anastomosis in the treatment of liver cirrhosis induced portal hypertension.</p><p><b>METHODS</b>Thirty-six patients admitted from January 2003 to December 2006 were randomly divided into splenic autotransplantation group undergoing splenic autotransplantation after splenectomy combined with lower esophagus transaction anastomosis, and splenectomy group only undergoing splenectomy combined with lower esophagus transaction anastomosis. The general conduction, splenic scanning, liver function, and the level of serum Tuftsin and IgM of each patient were observed before and after operation.</p><p><b>RESULTS</b>The levels of Tuftsin and IgM in splenic autotransplantation group were significant higher than that of splenectomy group 2 months after the operation, and the liver function showed no significant difference between these two groups. Splenic tissue was detected in the retroperitoneal space by 99mTc-DRBC 2 months after operation.</p><p><b>CONCLUSIONS</b>Splenic autotransplantation combined with lower esophagus transaction anastomosis is a safe and effective treatment strategy for patients with liver cirrhosis induced portal hypertension, and the spleen tissue transplanted into the retroperitoneal space can partially preserve the immune function.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Anastomosis, Surgical , Esophagus , General Surgery , Hypertension, Portal , General Surgery , Liver Cirrhosis , Spleen , Transplantation , Transplantation, Autologous , Treatment Outcome
13.
Chinese Journal of Surgery ; (12): 1892-1894, 2008.
Article in Chinese | WPRIM | ID: wpr-275926

ABSTRACT

<p><b>OBJECTIVE</b>To summarize the reasons for bile duct injury (BDI) after laparoscopic cholecystectomy (LC), and to determine the effect of multiple treatment after BDI.</p><p><b>METHODS</b>A retrospective cohort study was performed. The medical records of 110 patients diagnosed with BDI after LC from October 1993 to November 2007, in ten large hospitals in Guangdong of China, were reviewed.</p><p><b>RESULTS</b>Among 110 patients with BDI, 58 cases (52.7%) were local patients, whereas 52 cases (47.3%) were transferred from outside hospitals. Reasons for BDI following LC were: (1) Lack of experience of the LC operator (48.2%); (2) LC performed during acute cholecystitis (20.0%); (3) The structure of Calot triangle was unclear (15.5%); (4) Variable anatomical position (11.8%); (5) Intra-operation bleeding (4.5%). The commonest sites of injury were the choledochus and common hepatic duct (76.4%). Following BDI, endoscopic stenting or operative repair was performed in 106 patients. The overall success rate was 95.3% (101/106), with a mortality rate was 0.9% (1/106). Cholangitis occurred in 3.8% (4/106) cases. Choledocho-enterostomy operation was performed in almost 60.0% (63/106) cases, and the success rate was 93.7% (59/63). Endoscopic stenting or operative repair was performed immediately following BDI in 23.6% (25/106) patients, the success rate was 100%; and within 30 days in 63.2% (67/106) patients. Eighty-eight out of 106 patients who underwent repair were successful following the first operative procedure.</p><p><b>CONCLUSIONS</b>Factors such as an un-experienced operator and unclear anatomical position were causes of BDI following LC. Early operative repair should be regarded as the treatment of choice, in patients diagnosed with BDI. Early refer to an experienced hepatobiliary operator ensures a high success rate.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Bile Ducts , Wounds and Injuries , General Surgery , Cholecystectomy, Laparoscopic , Iatrogenic Disease , Intraoperative Complications , Diagnosis , General Surgery , Retrospective Studies
14.
Acta Academiae Medicinae Sinicae ; (6): 469-473, 2008.
Article in Chinese | WPRIM | ID: wpr-270667

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression profile of microRNAs during the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate.</p><p><b>METHODS</b>Total RNA was extracted from embryonic stem cells on day 0, 6, and 9 during cell differentiation, and microRNA was isolated from the total RNA. Microarray analysis of microRNA expression was performed to detect the different expression levels of microRNA among the indicated time points (day 0, 6, and 9).</p><p><b>RESULTS</b>Compared with the microRNA expression level on day 0 of cell differentiation, 17 different microRNAs exhibited higher expressions both on day 6 and day 9. Twenty-two and 27 microRNA demonstrated lower expressions on day 6 and day 9, respectively. Further analysis revealed that 15 microRNA among the above microRNAs with significant differential expression may keep close interation with histone deacetylase.</p><p><b>CONCLUSION</b>During the course of embryonic stem cells differentiation towards hepatocytes induced by sodium butyrate, histone deacetylase and its relevant microRNAs may play important roles in cell differentiation.</p>


Subject(s)
Animals , Mice , Butyrates , Pharmacology , Cell Differentiation , Cells, Cultured , Embryonic Stem Cells , Cell Biology , Metabolism , Gene Expression , Hepatocytes , Cell Biology , Metabolism , MicroRNAs , Genetics , Metabolism
15.
Chinese Journal of Gastrointestinal Surgery ; (12): 356-358, 2007.
Article in Chinese | WPRIM | ID: wpr-336446

ABSTRACT

<p><b>OBJECTIVE</b>To summarize the pathological classification, clinical symptom and experience in the diagnosis and treatment of primary tumor of small intestine.</p><p><b>METHODS</b>Data of 58 patients with primary tumor of small intestine pathologically confirmed from Oct. 1996 to Oct. 2006 were analyzed retrospectively.</p><p><b>RESULTS</b>Thirteen patient (22.4%) had primary benign tumors of small intestine and 45 patient (77.6%) had primary malignant tumors of small intestine. The major clinical signs of primary tumor of small intestine included hemorrhage(85%), abdomen pain(19%), abdomen mass and intestine obstruction(16%). Forty- eight patients (82.8%) were diagnosed by laparotomy of abdominal cavity and misdiagnosed preoperatively as other diseases.</p><p><b>CONCLUSIONS</b>Primary tumors of small intestine are difficult to be diagnosed preoperatively. CT scan, digital subtraction angiography and radionuclide imaging are helpful for the diagnosis. Laparotomy of abdominal cavity is the main choice for those patients with suspicious tumor of small intestine.</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Intestinal Neoplasms , Diagnosis , General Surgery , Intestine, Small , Pathology , Retrospective Studies
16.
Chinese Medical Journal ; (24): 452-457, 2007.
Article in English | WPRIM | ID: wpr-344875

ABSTRACT

<p><b>BACKGROUND</b>Surgical treatment options for patients with cirrhosis and portal hypertension are complicated. In this study, we evaluated the effectiveness of a new treatment strategy, splenic auto-transplantation and oesophageal transection anastomosis. We report results from clinical observations, splenic immune function and portal dynamics in 274 patients.</p><p><b>METHODS</b>From 1979 to 2005, 274 cirrhosis patients with portal hypertension underwent the new treatment strategy, and were followed up to compare results with those patients who underwent traditional surgical treatment. From 1999 to 2002, a randomized controlled trial (RCT) was performed on 40 patients to compare their post-operative immune function. From 1994 to 2006, another RCT enrolled 28 patients to compare portal dynamics using three-dimensional dynamic contrast-enhanced magnetic resonance angiography (3D DEC MRA) investigation post operation.</p><p><b>RESULTS</b>Among 274 patients (mean age 41.8 years), the emergency operative mortality (4.4%), selective operative mortality (2.2%), complication rate (17.9%), prevalence of hepatic encephalopathy (< 1%), rate of portal hypertension gastritis (PHG) bleeding (9.1%), and morbidity of hepatic carcinoma (8%) were similar to those patients undergoing traditional operation; the spleen immunology function (Tuftsin, IgM) decreased in both groups 2 months post operation, but this decrease did not reach statistical significance. Through 3D DCE MRA, the cross sectional area and the velocity and volume of blood flow of the main portal vein decreased significantly after operation in both groups. The velocity and volume of blood flow in the auto-transplantation group was significantly lower than that in the control group.</p><p><b>CONCLUSIONS</b>Splenic auto-transplantation and esophageal transection anastomosis is a safe, effective, and reasonable treatment strategy for patients with portal hypertension with varicial bleeding. It not only can correct hypersplenism, but may also achieve complete hemostasis. Spleens auto-transplanted into the retroperitoneal space can preserve immune function and establish broad collateral circulation.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Male , Middle Aged , Anastomosis, Surgical , Esophagus , General Surgery , Hypertension, Portal , Allergy and Immunology , General Surgery , Imaging, Three-Dimensional , Immunoglobulin M , Blood , Prospective Studies , Spleen , Transplantation , Transplantation, Autologous
17.
Chinese Journal of Hepatology ; (12): 726-729, 2004.
Article in Chinese | WPRIM | ID: wpr-250112

ABSTRACT

<p><b>OBJECTIVE</b>To study the role of a pathologic niche inducing mouse embryonic stem cells (ESC) to express hepatic cell functions in vitro.</p><p><b>METHODS</b>Embryoid bodies were developed from 5 to 7 day hanging-drop culture of mouse ESC, and their dissociated cells were planted in three differential systems: nothing added; with 20 ng/ml hepatocyte growth factor (HGF); and 5% rat cholestatic serum plus 20 ng/ml HGF added. Their differentiation was observed with inverted microscopes daily, and their hepatic functions were analyzed against their synthesis of glycogen, triglycerides, albumin, and urea nitrogen, and by their staining of indocyanine green (ICG) and fluorescein diacetate (FDA).</p><p><b>RESULTS</b>ESC spontaneous differentiation was hardly being controlled to form three germ layers. HGF prompted the ESC to develop further into visceral endoderm and mesoderm (myocardium), but both of them only expressed a low level of hepatocyte-specific metabolic functions. With cholestatic serum added into the HGF-induced system, differentiated cells grew into similar angular cells, and had a higher level synthesis of glycogen, triglycerides, albumin and urea nitrogen with positive ICG and FDA staining.</p><p><b>CONCLUSIONS</b>Spontaneous or HGF-induced ESC differentiation has only limited hepatic functions expressed. A pathologic niche in vitro induces ESC to develop into hepatic lineages, with a higher level of hepatic metabolic functions.</p>


Subject(s)
Animals , Mice , Cell Differentiation , Physiology , Cells, Cultured , Cholestasis , Blood , Culture Media , Pharmacology , Embryo, Mammalian , Hepatocytes , Cell Biology , Serum , Stem Cells , Cell Biology
18.
Chinese Journal of Analytical Chemistry ; (12): 606-610, 2001.
Article in Chinese | WPRIM | ID: wpr-410347

ABSTRACT

Micromachining of capillary electrophoresis chips is described.The current in channels was determined versus the voltage applied on the chip.Using laser-induced fluorescence detection,the process of injection and separation on chips was recorded with charge-coupled device(CCD).With defferent voltge of reservoirs on glass chips,profiles of the sample plug were studied. The elctropherogram of hydrolysis product of fluorescein isothiocyanate (FTTC-OH) and arginine labeled by fluorescein isothiocyante(FTTC-Arg) was obtained.

19.
Medical Journal of Chinese People's Liberation Army ; (12)2001.
Article in Chinese | WPRIM | ID: wpr-552275

ABSTRACT

To study the expression and significance of CD44 splice variant V6 in squamous cell carcinoma of the tongue. The expression levels of CD44v6 protein were determined immunohistochemically in 40 squamous cell carcinoma of the tongue specimens (24 non metastatic primary tumors and 16 metastatic lymph nodes), 12 squamous cell papilloma and 9 normal tongue mucosa using murine exon V6 specific monoclonal antibody. The CD44v6 overexpression was detectable in 29 of 40 squamous carcinoma of the tongue, in 2 of 12 squamous cell papilloma, in 0 of 9 mormal mucosa, P

20.
Journal of Interventional Radiology ; (12)1994.
Article in Chinese | WPRIM | ID: wpr-683018

ABSTRACT

Objective To evaluate the efficacy and safety of transarterial chemoembolization (TACE)combined with sonographically guided percutaneous microwave coagulation therapy(PMCT)for hepatic carcinoma with diameter>5.0 cm.Methods We retrospectively reviewed 68 cases of hepatic carcinoma with diameter>5.0 cm under treatment of TACE combined with PMCT.CT,USG and correlated laboratory tests of hepatic carcinoma were carried out.Results Among 68 cases,complete ablation were 5 cases(5/68),tumor ablation area more than 50% or tumor shrinkage less than 30% were 59 cases(59/ 68),tumor ablation area less than 50% or tumor shrinkage more than 30% were 6 cases(6/68).Forty five cases with high AFP descended more than 50% after the procedure in 42 eases(93.33%).Thirty seven cases and 29 cases with increase of CEA and CA19-9 decreased to 28(75.97%)and 23(93.10%)cases with corresponding index decreasing more than 50% respectively.Survival time reached 4-6 months in 3 cases, more than 6 months for 31 cases,more than 12 months of 34 cases.Two cases among them showed no recurrence up to now after stoppage of treatment for 24 months and finally no correlative mortality occurred. Conclusion TACE combined with sonographically guided PMCT for hepatic carcinoma with diameter more than 5 cm is safe and effective.

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