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1.
Chinese Medical Journal ; (24): 898-907, 2021.
Article in English | WPRIM | ID: wpr-878139

ABSTRACT

Pneumoconiosis refers to a spectrum of pulmonary diseases caused by inhalation of mineral dust, usually as the result of certain occupations. The main pathological features include chronic pulmonary inflammation and progressive pulmonary fibrosis, which can eventually lead to death caused by respiratory and/or heart failure. Pneumoconiosis is widespread globally, seriously threatening global public health. Its high incidence and mortality lie in improper occupational protection, and in the lack of early diagnostic methods and effective treatments. This article reviews the epidemiology, safeguard procedures, diagnosis, and treatment of pneumoconiosis, and summarizes recent research advances and future research prospects.


Subject(s)
Dust , Humans , Occupational Diseases , Occupational Exposure , Pneumoconiosis/epidemiology , Pulmonary Fibrosis
2.
Article in Chinese | WPRIM | ID: wpr-877083

ABSTRACT

Objective To establish a convenient and efficient method to detect Environmental endocrine disruptors (EDCs) in atmospheric particulate matter. Methods Atmospheric total suspended particles (TSP) was sampled through glass fiber filter paper in a medium-flow TSP sampler at a flow rate  of 100L/min for 2 hours. The filter paper sample was then subjected to the treatments as follows: (1) The samples were extracted with acetone and dichloromethane (3:2,v/v) from glass fiber filter paper. (2) Ultrasonic cleaning for 30 minutes. (3) Centrifugation at 2,500r/min for 20 minutes. (4) The supernatant was dried out in water baths at 55℃, and then acetonitrile was added to make the volume constant. Chromatographic condition was : HPLC-FLD ( λex=275nm,λem=312nm) . Results The content of OP, NP and BPA in the standard series showed a good linear relationship with their respective peak areas, and all the correlation coefficients were greater than 0.99. The detection limits for BPA, OP, NP were 9.80 ng/ml, 5.60 ng/ml, and 5.30 ng/ml, respectively, and the recoveries were 92.10%~127.00%, 83.90%~97.40%, and 83.70%~101.20%, respectively. The RSD for the inter-and intra-day was less than 5%. Conclusion The method was simple, rapid, and accurate, which could be used for the detection of environmental endocrine disruptors in atmospheric particulate matter.

3.
Acta Pharmaceutica Sinica ; (12): 1004-1009, 2020.
Article in Chinese | WPRIM | ID: wpr-821686

ABSTRACT

Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the content of cadmium (Cd) and arsenic (As) in earthworms. A physiologically-based extraction test (PBET) digestion in vitro /MDCK cell model was established to investigate the bioaccessibility of Cd and As in earthworms. The hazard index (HI) method and the margin of exposure (MOE) method were used to assess the risks of the total content and the bioaccessible content of Cd and As. The results showed that the total content of Cd and As in six batches of earthworms ranged from 8.319 to 33.606 mg·kg-1 and from 0.532 to 16.412 mg·kg-1, respectively. After uptake by MDCK cells, the bioaccessibility of Cd in earthworms ranged from 10.13% to 64.16%, and the bioaccessibility of As was from 2.72% to 46.57%. The results of risk assessment showed that before uptake by MDCK cells, the MOE values of As and HI values of Cd for all batches of earthworms were greater than 1, which suggests that the risks of As are acceptable but the risks of Cd are unacceptable. After transportation by MDCK cells, except for one batch of earthworms, the HI values of Cd in the other five batches were less than 1, which suggests that the risks are at a safe level. This study provides important technical support for a more objective and scientific assessment of the health risks of heavy metals in traditional Chinese medicines, and for a more scientific and reasonable standard limit of heavy metals.

4.
Article in Chinese | WPRIM | ID: wpr-746291

ABSTRACT

Objective To explore the strategies of promoting professional guidance,building a shared platform and effective performance evaluation,as well as dealing the problems of low utilization rate and repeated purchase of scientific research instruments and equipment faced by colleges and universities at the present stage,to provide further reference for the management of scientific research projects and equipment in Colleges and universities.Methods Taking A University as an example,identify and analyze the problems existing in the management efficiency of scientific instruments and equipment.proposed possible solutions based on relevant experience at home and abroad.Results Universities represented by A University are facing the main problems of low utilization rate of scientific research instruments and equipment and repeated purchases.Preliminary results have been achieved in solving these problems by promoting professional guidance,building a shared platform and effective performance evaluation.Conclusions The management of scientific research instruments and equipment in Colleges and universities needs to constantly innovate management methods and optimize the allocation of resources,promote the opening and sharing of scientific research instruments and equipment,enhance the efficiency of scientific research instruments and equipment management,and strengthen the management of scientific research funds.

5.
Article in Chinese | WPRIM | ID: wpr-705349

ABSTRACT

OBJECTIVE To have a systematic pathomechanism view of three chest impediment-syndromes of Qi Deficiency and Blood Stasis syndrome(QDBS),Qi Stagnation and Blood Stasis syn-drome (QSBS), Cold Obstruction and Qi Stagnation syndrome(COQS) and further investigate the changed metabolome and related pathways for screening potential biomarkers in rat plasma. METHODS According to clinical pathogeny, three kinds of syndrome models were established to simulate the disease of chest impediment. Plasma metabonomics based on UPLC-Q-TOF/MS was applied in this research to detected small molecule metabolites for identifyingthe special potential biomarkers of three chest impediment syndromes, respectively. RESULTS Significant metabolic differences were observed between thecontrol group and three syndrome groups. Furthermore, three syndrome groups were distinguished clearly by pattern recognition method.The particular metabolites contributing most to the classification of three chest impediment syndromes were identified. In the QSBS group, the potential biomarkers could include 2-keto-glutaramic acid, L-methionine, L-homocysteic acid, octadecanamide, stearoylglycine,behenic acid,linoleylcarnitine,lysoPC(14:1(9Z)),indoxyl sulfate and cholic acid.In the COQS group, they could be aminoadipic acid, palmitic amide, oleamide, lysoPC(P-16:0), lysoPC(P-18:0), lysoPC(20:2(11Z,14Z)), 9-HETE and tauroursodeoxycholic acid. Moreover, 4-pyridoxic acid, L-palmi-toylcarnitine, lysoPC(20:0), lysoPC (22:5 (4Z,7Z,10Z,13Z,16Z)), 3- hydroxyhexadecanoic acid and arachidonic acid could be the potential biomarkers for the QDBS group. CONCLUSION Three chest impediment syndromes have their own potential biomarkers.Each special metabolite has its owndifferent metabolic pathway.Both metabolismof cysteine and methionine,and metabolism of alanine,aspartate and glutamate are the main pathways in regulation of metabolic disorders in QSBS syndrome. Lysine biosynthesis and degradation,fatty acid metabolism,and glycerophospholipid metabolism are the main pathways in regulation of metabolic disorders in COQS syndrome.Arachidonic acid metabolism, fatty acid metabolism,fatty acid elongation in mitochondria,and vitamin B6 metabolism are the main pathways in regulation of metabolic disorders in QDBS syndrome.These endogenous substances were indicated as the special potential biomarkers for three chest impediment syndromes and worth studying in depth.

6.
Article in English | WPRIM | ID: wpr-690638

ABSTRACT

<p><b>OBJECTIVE</b>To explore whether the intake of dietary carotenoids could protect against skeletal fluorosis in Guizhou province in which coal-burning fluorosis is endemic.</p><p><b>METHODS</b>A case-control study of 196 patients with skeletal fluorosis and 196 age and gender-matched controls was conducted in Zhijin, Guizhou Province. Face-to-face interviews were conducted to assess habitual dietary intake using a 75-item food frequency questionnaire and various covariates with structured questionnaires. Urinary fluoride was measured using an ion-selective electrode method. The genotype of superoxide dismutase 2 (SOD2) rs11968525 was detected by TaqMan method.</p><p><b>RESULTS</b>We observed significant dose-dependent inverse associations of skeletal fluorosis with intake of β-carotene, lutein/zeaxanthin, lycopene, and total carotenoids (P-trend = 0.002 to 0.018), whereas α-carotene and β-cryptoxanthin intakes were not found to be related to skeletal fluorosis, after adjustment for potential confounders. The adjusted ORs and 95% CI of skeletal fluorosis for the highest versus lowest quartile were 0.30 (0.10, 0.86) for β-carotene, 0.23 (0.08, 0.66) for lycopene, 0.26 (0.10, 0.75) for lutein/zeaxanthin and 0.34 (0.14, 0.74) for total carotenoids (all P-trend < 0.05). Stratified analyses showed that the protective effects of lutein/zeaxanthin and total carotenoids on skeletal fluorosis were more evident for individuals with the AG+AA genotypes of SOD2 (rs11968525).</p><p><b>CONCLUSION</b>Increased intakes of β-carotene, lutein/zeaxanthin, lycopene, and total carotenoids are independently associated with a lower risk of coal-burning skeletal fluorosis. SOD2 (rs11968525) polymorphisms might modify the inverse associations between dietary carotenoids and skeletal fluorosis.</p>


Subject(s)
Bone Diseases, Metabolic , Genetics , Urine , Carotenoids , Case-Control Studies , China , Coal , Energy Intake , Environmental Exposure , Feeding Behavior , Female , Fluoride Poisoning , Genetics , Urine , Fluorides , Urine , Humans , Middle Aged , Polymorphism, Genetic , Superoxide Dismutase , Genetics , Surveys and Questionnaires
7.
Chongqing Medicine ; (36): 4036-4039, 2017.
Article in Chinese | WPRIM | ID: wpr-662206

ABSTRACT

Objective To investigate the effects of nonyphenol (NP)exposure during pregnant and lactation period on expression of cytochrome P450 2E1 (CYP2E1) mRNA and protein of hepatic tissues in offspring rats.Methods Pregnant rats were assigned to four groups:the exposure groups received gavage with NP at dose levels of 50 mg · kg-1 · d-1 (low dose group),100 mg · kg-1 · d-1 (medium dose group),200 mg · kg-1 · d-1 (high dose group) and the control group was treated with corn oil alone,NP exposure time was limited from gestational day 6 to postnatal day 21.The newborn rats were sacrificed at 90 days after birth,followed by blood collection and serum separation.Then,serum biochemical indicators of liver function and lipid levels were detected.Glutathione peroxidase (GSH-PX),superoxide dismutase (SOD) activities,malondialdehyde (MDA) level and CYP2E1 mRNA and protein levels were determined in hepatic tissues.Pathologic changes in hepatic tissues were observed with HE staining.Results Compared with the control group,the aspartate aminotransferase (ALT),alanine aminotransferase (AST) levels and AST/ALT ratio of offspring rats in exposure groups were increased in a dose-dependent manner (P<0.05),as well as serum TG,TC and LDL-C levels were increased (P<0.05).The liver tissue structure of the control group was normal.The hepatic sinus of the medium dose group was showed mild expansion and inflammatory cellular infiltration.Otherwise,the liver of high dose group had a large amount of lipid droplets.Compared with the control group,SOD and GSH-PX activities were obviously decreased,while MDA level was significantly decreased in each exposure group (P<0.05).CYP2E1 mRNA and protein expression levels of medium and high dose groups were higher than those in the control group (P<0.05).Conclusion Exposure to NP during gestation and lactation period can induce lipid metabolism disorders and inflammatory lesions in hepatic tissues of offspring rats,and it maybe associated With up-regulation of CYP2E1 expression.

8.
Tianjin Medical Journal ; (12): 1057-1060, 2017.
Article in Chinese | WPRIM | ID: wpr-657717

ABSTRACT

Objective To compare the clinical efficacy between the vaginal ovarian cystectomy and the laparoscopic ovarian cystectomy. Methods A total of 104 patients with ovarian cyst hospitalized in our hospital during June 2013-June 2016 were selected and divided into observation group (vaginal ovarian cystectomy, n=55) and control group (laparoscopic ovarian cystectomy, n=49). There were no significant differences in mean age, body mass index (BMI), abdominal surgical history, cyst diameter and cyst type between the two groups. The operation time, blood loss in operation, hospitalization expenses and average hospital stay were observed in the two groups. The ovarian reserve function before and after the operation was also observed. Results The operation time [(46.9±18.4 min)], hospitalization cost [(8165.3±951.8) yuan] and the average length of hospital stay (4.5 ± 1.9 days) were significantly less in the observation group than those of the control group [(62.3 ± 26.5) min], [(12543.9 ± 1464.4) yuan] and [(6.8 ± 2.3) days], (P<0.05). Before the operation, there were no significant differences in estradiol (E2), follicle stimulating hormone (FSH)/luteinizing (LH), FSH and antral follicle count (AFC) between the two groups. After the operation, the values of FSH/LH (1.4±0.4 vs. 1.8±0.6) and FSH [(9.2±2.9) U/L vs. (13.6±4.3) U/L] were significantly decreased in the observation group than those of the control group. The AFC (7.2±1.6 vs. 6.0±1.3) was significantly more in the observation group than that of the control group (P<0.05). Conclusion The vaginal ovarian cystectomy is of little damage to ovarian reserve function, which is suitable for women of child-bearing age and conforms to the requirement of"value medicine".

9.
Tianjin Medical Journal ; (12): 1057-1060, 2017.
Article in Chinese | WPRIM | ID: wpr-660078

ABSTRACT

Objective To compare the clinical efficacy between the vaginal ovarian cystectomy and the laparoscopic ovarian cystectomy. Methods A total of 104 patients with ovarian cyst hospitalized in our hospital during June 2013-June 2016 were selected and divided into observation group (vaginal ovarian cystectomy, n=55) and control group (laparoscopic ovarian cystectomy, n=49). There were no significant differences in mean age, body mass index (BMI), abdominal surgical history, cyst diameter and cyst type between the two groups. The operation time, blood loss in operation, hospitalization expenses and average hospital stay were observed in the two groups. The ovarian reserve function before and after the operation was also observed. Results The operation time [(46.9±18.4 min)], hospitalization cost [(8165.3±951.8) yuan] and the average length of hospital stay (4.5 ± 1.9 days) were significantly less in the observation group than those of the control group [(62.3 ± 26.5) min], [(12543.9 ± 1464.4) yuan] and [(6.8 ± 2.3) days], (P<0.05). Before the operation, there were no significant differences in estradiol (E2), follicle stimulating hormone (FSH)/luteinizing (LH), FSH and antral follicle count (AFC) between the two groups. After the operation, the values of FSH/LH (1.4±0.4 vs. 1.8±0.6) and FSH [(9.2±2.9) U/L vs. (13.6±4.3) U/L] were significantly decreased in the observation group than those of the control group. The AFC (7.2±1.6 vs. 6.0±1.3) was significantly more in the observation group than that of the control group (P<0.05). Conclusion The vaginal ovarian cystectomy is of little damage to ovarian reserve function, which is suitable for women of child-bearing age and conforms to the requirement of"value medicine".

10.
Chongqing Medicine ; (36): 4036-4039, 2017.
Article in Chinese | WPRIM | ID: wpr-659571

ABSTRACT

Objective To investigate the effects of nonyphenol (NP)exposure during pregnant and lactation period on expression of cytochrome P450 2E1 (CYP2E1) mRNA and protein of hepatic tissues in offspring rats.Methods Pregnant rats were assigned to four groups:the exposure groups received gavage with NP at dose levels of 50 mg · kg-1 · d-1 (low dose group),100 mg · kg-1 · d-1 (medium dose group),200 mg · kg-1 · d-1 (high dose group) and the control group was treated with corn oil alone,NP exposure time was limited from gestational day 6 to postnatal day 21.The newborn rats were sacrificed at 90 days after birth,followed by blood collection and serum separation.Then,serum biochemical indicators of liver function and lipid levels were detected.Glutathione peroxidase (GSH-PX),superoxide dismutase (SOD) activities,malondialdehyde (MDA) level and CYP2E1 mRNA and protein levels were determined in hepatic tissues.Pathologic changes in hepatic tissues were observed with HE staining.Results Compared with the control group,the aspartate aminotransferase (ALT),alanine aminotransferase (AST) levels and AST/ALT ratio of offspring rats in exposure groups were increased in a dose-dependent manner (P<0.05),as well as serum TG,TC and LDL-C levels were increased (P<0.05).The liver tissue structure of the control group was normal.The hepatic sinus of the medium dose group was showed mild expansion and inflammatory cellular infiltration.Otherwise,the liver of high dose group had a large amount of lipid droplets.Compared with the control group,SOD and GSH-PX activities were obviously decreased,while MDA level was significantly decreased in each exposure group (P<0.05).CYP2E1 mRNA and protein expression levels of medium and high dose groups were higher than those in the control group (P<0.05).Conclusion Exposure to NP during gestation and lactation period can induce lipid metabolism disorders and inflammatory lesions in hepatic tissues of offspring rats,and it maybe associated With up-regulation of CYP2E1 expression.

11.
Article in Chinese | WPRIM | ID: wpr-496005

ABSTRACT

Diabetic foot is one of the chronic complications of diabetes,which has great harm.Through active treatment such as anti-infection,debridement,surgical intervention,amputation rate can be declined.

12.
Article in Chinese | WPRIM | ID: wpr-479975

ABSTRACT

Objective To investigate the risks and benefits of interventions promoting placental transfusion (PT) at delivery [delayed cord clamping (DCC) or umbilical cord milking (UCM)] compared with early cord clamping (ECC) on outcomes among very low birth weight infants (VLBWI).Methods A systematic search was conducted of PubMed, EMBASE, ClinicalTrials.gov, China Academic Journal Network Publishing Database and Wanfang Medical Databases (January 1965 to July 2014) for randomized controlled trial (RCT) articles relating to PT strategies (DCC and UCM) in VLBWI.The Cochrane Handbook 5.1.0 was used to evaluate the methodological quality and RevMan 5.3 software from Cochrane Collaboration was used for metaanalysis.The fixed effect or random effect model was adopted according to the result of heterogeneity, Results We identified 14 eligible studies describing a total of 659 neonates with an average birth weight < 1 500 g.There were eight studies for DCC and six studies for UCM.Compared with the control, benefits of greater PT decreased any grade intraventricular hemorrhage (IVH) (nine studies, OR=0.49, 95%CI: 0.32-0.77, P < 0.01), increased the blood pressure at four hours of life (eight studies, MD=4.42, 95%CI: 3.85-4.98, P < 0.01), and also showed higher initial hemoglobin (Hb) level (six studies, MD=3.52, 95%CI: 1.67-5.37, P < 0.01) and lower incidence of sepsis during the hospital stay (five studies, OR=0.46, 95%CI: 0.26-0.83, P=0.01).No differences were observed between the groups about the fllowing indicators (all P > 0.05): 5-minute Apgar scores (MD=0.01,95%CI:-0.21-0.22), admission temperature (MD=0.13, 95%CI:-0.15 to 0.41), peak serum bilirubin levels (MD=0.59, 95%CI:-0.13 to 1.31), initial reported hematocrit (MD=3.48, 95%CI:-0.46 to 7.43), mortality before discharge (OR=0.63, 95%CI:0.31-1.25), Bell's stage 2 or greater necrotizing enterocolitis (OR=0.62 ,95%CI: 0.29-1.33), rates of transfusion due to anemia (OR=0.63, 95%CI: 0.35-1.15) and oxygen therapy at 36 weeks of corrected age (OR=0.79, 95%CI: 0.46-1.34).Conclusions It is suggested that enhanced PT (both DCC and UCM) at birth is safe and provide better neonatal outcomes than ECC for those VLBWI, most notably reduces the overall IVH occurrence and lower the incidence of sepsis.The optimal umbilical cord clamping practice and UCM among VLBWI infants remains uncertain and long-term neurodevelopmental outcomes are warranted.

13.
Article in Chinese | WPRIM | ID: wpr-484004

ABSTRACT

Objective To study the clinical characteristics of silent paraganglioma ( PGL) .Methods A total of 21 patients with silent PGL confirmed by surgical pathology and hospitalized in the First Affiliated Hospital of chongqing Medical University from Mar .2008 to Dec.2013 were enrolled in this study .The clinical manifesta-tions, hormone level, imaging findings, pathologic characteristic, treatment and follow-up data were reviewed and analyzed retrospectively .Results Among the 21 cases of silent PGL , 8 were male and 13 were female, with 47.05 years as the mean age(ranging from 26 to 67 years old).PGL tumors in silent group were mainly located in the right side, and left to right ratio was 2∶5.Silent PGL with tumor diameter 5 cm were 5, 7,and 9 cases respectively.Its constituent ratio was 23.8%, 33.3% and 42.9% respectively, significantly higher compared with that of symptomatic PGL (9.8%,43.9%,and 46.3%respectively).Silent PGL can be i-dentified from other adrenal incidentaloma based on enhanced CT value in each period .Biochemical examination or functional imaging further could facilitate the diagnosis of silent PGL .All the 21 cases were conducted with surgical treatment , and hypertensive crisis occurred to 4 cases during operation .Conclusions Silent PGL is not easy to be detected due to its non-specific clinical manifestations .The diagnosis needs the combined analysis on anatomical imaging characteristics , biochemical examination , functional imaging and pathological findings .Sur-gery is the most effective treatment .Postoperative pathological tips and long-term follow-up are both required for PGL, no matter benign or malignant .

14.
Modern Clinical Nursing ; (6): 34-36, 2013.
Article in Chinese | WPRIM | ID: wpr-441074

ABSTRACT

Objective To investigate the clinical nursing path on the psychological status of cerebral infarction patients hospitalized for the first time.Methods Two hundred cerebral infarction patients hospitalized for the first time were equally randomized into the observation group and control group:the former used conventional nursing method and the latter a clinical nursing path.The self-rating depression scale(SDS)and self-rating anxiety scale(SAS)were used to assess their psychological state.Results After intervention,the scores on SDS and SAS in the former group were significantly lower than those in the latter group(all P<0.05). The satisfaction rate in the former was higher than that of the control(P<0.05).Conclusion The clinical nursing path makes it possible to effectively alleviate the psychological burden of the cerebral infarction patients hospitalized for the first time and improve the patients’satisfaction with nursing working.

15.
Chinese Journal of Biotechnology ; (12): 800-812, 2012.
Article in Chinese | WPRIM | ID: wpr-342440

ABSTRACT

Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the first and rate-limiting step of the oxidative pentose phosphate pathway, existing in both cytosolic and plastidic compartments of higher plants. Its main function is to provide reducing power (NADPH) and pentose phosphates for reductive biosynthesis and maintenance of the redox state of the cell. In addition, the expression of this enzyme is related to different biotic and abiotic stresses. In this review, we analyzed the isoenzyme, regulation and biological function of G6PDH. Meanwhile, we summarized the progress work of G6PDH involved in stress resistance, gene cloning, enzyme-deficiency and cluster analysis. Problems should be solved were also discussed.


Subject(s)
Amino Acid Sequence , Glucosephosphate Dehydrogenase , Genetics , Metabolism , Physiology , Isoenzymes , Molecular Sequence Data , Pentose Phosphate Pathway , Physiology , Plants , Metabolism
16.
Article in Chinese | WPRIM | ID: wpr-840565

ABSTRACT

Objective: To observe the influence of spent culture supernatant (SCS) of Lactobacillus acidophilus strain LA14 on the contraction of isolated intestinal smooth muscle and to discuss the related mechanism. Methods: The ileum samples of rabbits were prepared and the contraction frequency and amplitude of intestinal smooth muscle were observed as the normal control. Then the SCS, bacterium suspension, and SCS with bacterium suspension were added by an accumulative dose to the culture media (0.3 ml per times, at an interval of 6 min), respectively. Four minutes after each administration, the contractive curves were recorded for 2 min. The influences of various groups of Lactobacillus acidophilus on the contraction of isolated intestinal smooth muscle were observed. The effect of SCS on M cholinoceptor was observed by adding in order pilocarpine, atropine or SCS, and pilocarpine. Results: After continuous administration of SCS or SCS with bacterium suspension (0.6-1.5 ml), the contraction frequency of the intestinal smooth muscle was significantly lowered compared with before administration (P0.05). Within the range of 0.3-1.5 ml, the SCS, bacterium suspension, and SCS with bacterium suspension resulted in no significant difference in reducing the contraction amplitude, except for SCS with bacterium suspension at 1.5 ml(P<0.05). SCS or atropine significantly inhibited pilocarpine-induced increase of contraction amplitude(P<0.05 or P<0.01). SCS also reduced the contraction frequency of the intestinal smooth muscle(P<0.01). Conclusion: SCS of Lactobacillus acidophilus may inhibit the peristalsis of the intestinal smooth muscle of rabbits by blocking M cholinoceptor.

17.
Article in Chinese | WPRIM | ID: wpr-248300

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the transformation of microhardness and microstructures of human dental fluorosis after demineralization and remineralization in vitro.</p><p><b>METHODS</b>Forty human dental fluorosis enamel blocks were demineralized by acid-etching gel, then subjected to 1.0% casein phosphopeptides stabilized calcium phosphate (CPP-CP) for remineralization. Surface enamel microhardness was measured on the enamel blocks before and after demineralization and after remineralization. The enamel specimens was observed by stereomicroscope and scanning electron microscope, then sectioned (100 microm) and examined by transmission light microscope and polarized light microscope.</p><p><b>RESULTS</b>Before demineralization, the average knoop hardness value of the fluorosed teeth was (241.53 +/- 21.31) kHV. After demineralization, obvious decrease of the surface hardness of the enamel was observed, the mean value was (175.76 +/- 24.99) kHV (P < 0.05), the percent of the surface microhardness demineralization (% SMHD) was (27.23 +/- 1 4.79)%, and major demineralization was under the surface of the enamel, which was similar to the early natural enamel caries. After remineralization, obvious increase of the surface hardness of the enamel was observed, the mean value was (210.17 +/- 21.48) kHV (P < 0.05), the percent of the surface microhardness remineralization (% SMHR) was (52.32 +/- 4.23)%, major remineralization was under the surface of the enamel.</p><p><b>CONCLUSION</b>Remineralization could be used to prevent and cure the early natural enamel caries of fluorosed dental teeth.</p>


Subject(s)
Calcium Phosphates , Cariostatic Agents , Caseins , Dental Caries , Dental Enamel , Hardness , Humans , In Vitro Techniques , Tooth Demineralization , Tooth Remineralization
18.
Chinese Journal of Hematology ; (12): 394-398, 2009.
Article in Chinese | WPRIM | ID: wpr-314473

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the frequency of JAK2 V617F mutation in 145 myeloproliferative disorders (MPDs) patients, analyze the correlation between JAK2 V617F mutation and clinical features.</p><p><b>METHODS</b>The JAK2 V617F mutation was detected by direct DNA sequencing of PCR product and allele-specific PCR respectively. The expression of JAK2, phospho-JAK2 and phospho-STAT5 proteins was determined by Western blot. The clinical data of MPDs patients with or without JAK2 V617F mutation was collected and analyzed for evaluating the clinical significance of JAK2 V617F mutation.</p><p><b>RESULTS</b>1) The frequency of JAK2 V617F mutation for PV, IMF, ET was 92%, 58%, 50% respectively. Compared with conventional DNA sequencing (PV 84%, IMF 44%, ET 39%, respectively), allele-specific PCR exhibited a higher sensitivity in JAK2 V617F mutation detection. 2) The expression levels of phospho-JAK2 and phospho-STAT5 in peripheral blood mononuclear cells (PBMNCs) were upregulated significantly in JAK2 V617F-positive patients than in JAK2 V617F negative patients. 3) Compared with the patients with no JAK2 V617F mutation, the JAK2 V 617F-positive patients' features were as follows: older age of onset, higher mean leukocyte counts, lower platelet counts and smaller spleen volume. Frequency of thrombosis events in PT, ET, IMF was 17%, 32%, 16% respectively for JAK2 V617F positive group, and 0% (PV), 16% (ET), 5% (IMF) for JAK2 V617F negative group.</p><p><b>CONCLUSIONS</b>MPDs patients display higher frequency of JAK2 V617F mutation. JAK2 V617F mutation positive patients predispose to a thrombosis tendency.</p>


Subject(s)
Female , Humans , Janus Kinase 2 , Genetics , Metabolism , Male , Mutation , Myeloproliferative Disorders , Genetics , Metabolism , Phosphorylation , STAT5 Transcription Factor , Metabolism , Sequence Analysis, DNA
19.
Chinese Journal of Epidemiology ; (12): 441-444, 2007.
Article in Chinese | WPRIM | ID: wpr-294319

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influencing factors on cerebral stroke in Zhangwu county, Liaoning province, a region with high hypertension prevalence rate.</p><p><b>METHODS</b>By cluster sampling method, 5208 adults ( > 18y. ) from 11 villages of 6 towns were registered. General information, common risk factors of cerebral stroke about these persons was recorded. Blood pressure together with several biochemistry indicators was determined. Data were analyzed by SPSS 10.0 software.</p><p><b>RESULTS</b>Standardized prevalence of cerebral stroke in this region was 3.10%, and the difference between males and females was significant. Prevalence rate was increasing with age. Multiple-factor analysis revealed that the incidence rate of cerebral stroke was related to high blood pressure, high diastolic pressure, pulse pressure, age, high-density lipoprotein cholesterol (HDL-C) and low-DL-C (LDL-C) level with OR values of 95% CI as 2.958 (1.783-4.907), 2.803 (1.934-4.062), 1.154 (1.056-1.261), 1.080 (1.063-1.097), 0.390 (0.235-0.647) and 1.422 (1.008-2.006) respectively.</p><p><b>CONCLUSION</b>High blood pressure, in particular high diastolic pressure, pulse pressure and LDL-C level were main risk factors of cerebral stroke in Zhangwu countryside. However, HDL-C level was a protective factor. No new risk factors were discovered.</p>


Subject(s)
Adult , Aged , Blood Pressure , Physiology , China , Epidemiology , Cholesterol, HDL , Blood , Cholesterol, LDL , Blood , Female , Humans , Hypertension , Epidemiology , Male , Middle Aged , Risk Factors , Stroke , Blood , Epidemiology , Young Adult
20.
Chinese Journal of Epidemiology ; (12): 761-764, 2006.
Article in Chinese | WPRIM | ID: wpr-261748

ABSTRACT

<p><b>OBJECTIVE</b>To study the relationship between serum iron(SI) and essential hypertension (EHT) based on population-based samples.</p><p><b>METHODS</b>Using clustering multistage sampling method, all the people above 18 years old in the target population were investigated. Blood pressure was measured and the questionnaire was used to find out related factors. Five milliliters fast vein blood were drawn and the serum were used for testing on serum iron (SI) and other elements such as blood sugar, cholesterol (CHOL), triglyceride (TG), high density lipoprotein(HDL-C), low density lipoprotein (LDL-C), serum sodium, serum potassium, serum calcium etc. A case control study was carried out with EHT patients from the selected population as case group, and the other healthy peoples as controls. Database was created by Fox Pro and SPSS 10.0 was used for statistical analysis.</p><p><b>RESULTS</b>The concentrations of SI, with (17.75 +/- 7.66) micromol/ L in EHT group and (17.23 +/- 7.83) micromol/L in control group, showed statistical difference (P < 0.05) between the two groups. The concentrations of SI also showed statistical difference (P < 0.05) between the high DBP and normal group with the average level as (17.84 +/- 7.58) micromol/L in high DBP group and (17.26 +/- 7.85) micromol/L in normal group. Data from monovariate analysis showed that the increase of SI was a risk factor for EHT, DBP and SBP. By multivariate analysis for EHT, while SI still existed in the model (OR = 1.296, 95% CI: 1.057-1.590), but for SBP the results almost remained the same (OR = 1.285, 95% CI:1.102-1.498).</p><p><b>CONCLUSION</b>Data from the results showed that SI was probably a risk factor for EHT.</p>


Subject(s)
Case-Control Studies , China , Epidemiology , Humans , Hypertension , Blood , Epidemiology , Iron , Blood , Risk Factors
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