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1.
China Pharmacy ; (12): 1982-1987, 2021.
Article in Chinese | WPRIM | ID: wpr-886582

ABSTRACT

OBJECTIVE:To establish th e fingerp rint and c onduct cluster analysis of Hefu zhiyang decoction (HFZYD),and establish the method for content determination of 8 components,so as to provide reference for the quality control of HFZYD. METHODS:UPLC method was adopted. The determination was performed on ACQUITY UPLC BEH C 18 column with mobile phase consisted of acetonitrile- 0.1% glacial acetic acid solution (gradient elution )at the flow rate of 0.25 mL/min. The column temperature maintained at 30 ℃,and detection wavelength was 236 nm. The sample size was 5 μL. The fingerprint of 10 batches of HFZYD was established with Similarity Evaluation System of TCM Chromatographic Fingerprints (2012 edition). Compared with substance control ,the common peaks were identified. The similarity evaluation was conducted. SPSS 21.0 software was used to conduct cluster analysis of 10 batches of samples. The contents of 8 components such as gallic acid in 10 batches of samples were determined by above UPLC. RESULTS :There were 34 common peaks in the UPLC fingerprint of 10 batches of HFZYD , identified as peak 1 was gallic acid ,peak 10 was ferulic acid ,peak 13 was astilbin ,peak 23 was paeonol ,peak 28 was dictamnine,peak 31 was obacunone ,peak 32 was fraxinellone ,and peak 34 was osthole. Its similarity with the control fingerprint was 0.923-0.979. Among 10 batches of samples ,S1,S2,S5,S6,S7,S8 and S 10 were clustered into one category ,and S 3,S4 and S 9 were clustered into one category. The average contents of above 8 components were 0.596-0.714,0.262-0.321,7.647-9.859, 0.113-0.644,0.170-0.202,0.854-1.281,0.631-0.857,3.243-3.548 mg/g,respectively. CONCLUSIONS :UPLC fingerprint and the method for content determination of 8 components in HFZYD are established successfully.

2.
China Pharmacy ; (12): 1616-1622, 2021.
Article in Chinese | WPRIM | ID: wpr-881465

ABSTRACT

OBJECTIVE:To investigate and study the problems in the items labeling of Chinese patent medicine instructions in National Essential Medicines List ,and to provide reference for rational drug use and instruction revision. METHODS :Totally 268 Chinese patent medicines contained in the 2018 edition of National Essential Medicines List ,including 465 instructions of different . An dard docetaxel as first-line therapy in patients with meta - economic evaluation of docetaxel and paclitaxel regimens in metastatic breast cancer in the UK[J]. Pharmacoeco - dosage forms ,were collected ,and the labeling rate and content problems of drug instructions were counted. RESULTS :Of the 465 instructions,86.88% were labeled with cautions. The labeling rates of ADR ,pharmacokinetics,contraindications,drug interactions , clinical trials ,pharmacological toxicology and pharmacological action were low ,being 20.64%,0.21%,30.1%,1.07%,2.36%, 8.81%,1.93%. All other items were labeled. Incomplete description of ingredients ,incomplete information of decoction pieces and excipients accounted for 2.15% and 48.81%,respectively;the expression of functional indications was varied ,and there were 12 expression methods ,among which 16.5% of indications lacked TCM indications ;specifications were not uniform ,and there were 5 expression methods ;usage and dosage were not clearly marked ,and 465 instructions did not mention the usage and dosage for the elderly or pregnant and lactating women. Drug interactions ,ADR,matters needing attention and contraindications were not fully described with no substantive content ;only 0.64% marked interaction with chemical medicine and 12.68% marked contraindication content. CONCLUSIONS :There are some problems in the description of Chinese patent medicine instructions in 2018 edition of National Essential Medicine List ,such as non-standard and incomplete. It is suggested to strengthen efforts to establish and improve the information collection and feedback mechanism of Chinese patent medicine ,and introduce policies to support enterprises to carry out basic and clinical research after listing ;and through actively carrying out basic and clinical research in line with the characteristics of traditional Chinese medicine ,guided by the theory of traditional Chinese medicine ,combined with the latest research results ,so as to further improve the contents of Chinese patent medicine instructions.

3.
Article in Chinese | WPRIM | ID: wpr-905760

ABSTRACT

information (31.3%) was the lowest. The reporting rate was less as the guidelines published in China than in foreign contries (OR = 0.80, 95%CI 0.56-1.16), in original version than in update version (OR = 0.79, 95%CI 0.54-1.16); and higher as developed by various societies or associations than developed by non-societies or associations (OR = 1.15, 95%CI 0.82-1.61), however, no statistically significant difference was found in abovecomparisons. Objective:To evaluate the quality of reporting of clinical practice guidelines of rehabilitation. Methods:A comprehensive retrieve was performed in electronic databases of PubMed, EMBASE, CNKI, China Biology Medicine disc, Wanfang data, etc., from January 1, 2017 to January 11, 2020. Supplementary searches had been done on relevant websites. Two researchers reviewed literatures and assessed the reporting quality independently by using Reporting Items for Practice Guidelines in Healthcare (RIGHT), and any disagreements needed to be discussed in a consensus meeting. Results:A total of 16 guidelines were included, with an average reporting rate of (44.8±27.9)%. Among the seven domains of RIGHT, basic information was reported the highest (57.3%), and evidence (31.3%) and other Conclusion:Current clinical practice guidelines of rehabilitation reported with low quality. It is proposed that future guideline developers should report guidelines after RIGHT statements, including key information and content, in order to improve the quality of reporting guidelines.

4.
Article in Chinese | WPRIM | ID: wpr-873299

ABSTRACT

Objective::To study the spectrum-effect relationship between HPLC fingerprints and anti-hepatoma activity of Paris polyphylla var. yunnanensis, P. forrestii and P. vietnamensis, and to elucidate its effective substance. Method::HPLC was used to establish the fingerprint of three extracts from the plant. The mobile phase was consisted of acetonitrile (A)-water (B) for gradient elution (0-10 min, 20%A; 10-20 min, 20%-25%A; 20-30 min, 25%-30%A; 30-40 min, 30%-35%A; 40-50 min, 35%-40%A; 50-60 min, 40%A; 60-75 min, 40%-45%A; 75-80 min, 45%-60%A), and the flow rate was 0.9 mL·min-1. The UV detection wavelength was 203 nm. Thiazolyl blue tetrazolium bromide (MTT) array was used to detect the inhibitory effects of three extracts on the proliferation of HepG2 cells, and the half inhibitory concentration (IC50) was calculated. Cluster analysis and grey relational analysis were used to analyze the data of spectrum and efficacy, and to find out the components that contributed a lot to the anti-liver cancer effect. Result::A total of 11 common peaks were identified as common peaks among HPLC fingerprints of three kinds of Paris. After treated 72 h, P. forrestii has the highest inhibitory effect on the HepG2 cells, the IC50 of P. forrestii, P. polyphylla var. yunnanensis and P. vietnamensis were 148.33, 178.87, 208.09 mg·L-1, respectively. According to the grey relational analysis, the common peaks 1-10 from P. polyphylla var. yunnanensis had great correlation to anti-tumor effect, and the common peaks 1-7 for P. forrestii, the common peaks 1-4, 6-10, N1 for P. vietnamensis, all the correlation degrees with IC50 were >0.7.Cluster analysis of variables in each Paris showed that peaks with correlation degree >0.7 could cluster with IC50. Conclusion::The established HPLC fingerprint method is reliable with good reproducibility. The peaks 1-4, 6 and 7 from three kinds of Paris have the greatest contribution to the anti-hepatoma effect.

5.
International Eye Science ; (12): 576-579, 2020.
Article in Chinese | WPRIM | ID: wpr-798305

ABSTRACT

@#AIM: To compare the effect of platelet-rich fibrin(PRF)grafts and conjunctival-limbal autografts in pterygium excision.<p>METHODS: Totally 62 cases(62 eyes)of primary pterygium were randomly divided into group 1(32 eyes)and group 2(30 eyes). After pterygium excision, conjunctival-limbal autografts was performed in group 1 and PRF grafts was performed in group 2. PRF was prepared by centrifugation and compression of venous blood from patients before the surgery. The follow-up period was 6mo. The surgery time, complications and recurrence rate were evaluated and compared between the two groups.<p>RESULTS: The operation time of group 1 and group 2 were 27.3±4.3min and 22.0±4.0min respectively. There was significant difference between the two groups(<i>t</i>=4.990, <i>P</i><0.01). Conjunctival granuloma developed only in one eye in group 2 after the surgery, and there was no significant difference between group 1 and group 2(<i>P</i>=0.484). Pterygium recurred in one eye in group 1 and in one eye in group 2. There was also no significant difference in recurrence rate between group 1 and group 2(<i>P</i>=1.000). There was no significant difference in intraocular pressure between group 1 with 14.69±2.44mmHg and group 2 with 14.96±2.93mmHg before the surgery(<i>t</i>=0.399, <i>P</i>=0.691). As well, there was no significant difference in intraocular pressure between group 1 with 14.68±1.65mmHg and group 2 with 15.11±2.12mmHg after the surgery(<i>t</i>=0.888, <i>P</i>=0.378). The dissolution time of PRF membrane in PRF grafts group was 3.5±0.8d.<p>CONCLUSION: The use of PRF in pterygium surgery is safe, effective, easier and timesaving. It's a promising method for clinical application.

6.
Article in Chinese | WPRIM | ID: wpr-846184

ABSTRACT

Objective: To explore the content of seven active compounds in 10 kinds of medicinal herbs of Paridis Rhizoma, and to carry out chemical composition integration evaluation, which provides a scientific basis for its resource utilization. Methods: A total of 55 batches of medicinal herbs were collected from different areas, and their saponins I, saponins II, saponins VI, saponins VII, diosgenin, saponins H, and saponins were determined by ultra performance liquid chromatography. Then, TOPSIS model was used to normalize the content results and integrate the multi-indicator data to obtain a comprehensive index of content of seven active compounds. Results: The 10 kinds of medicinal herbs of Paridis Rhizoma were ranked from long to low was Paris forrestii (Ci = 0.275 5) > Paris polyphylla (Ci = 0.273 2) > Paris daliensis (Ci = 0.269 8) > Paris polyphylla var. yunnanensis (Ci = 0.244 5) > Paris vietnamensis (Ci = 0.234 5) > Paris polyphylla var. stenophylla (Ci = 0.159 1) > Paris thibetica (Ci = 0.141 6) > Paris polyphylla var. nana (Ci = 0.117 8) > P. vietnamensis (Ci = 0.115 1) > Paris mairei (Ci = 0.114 9), indicating that comprehensive quality of 10 kinds of medicinal herbs of Paridis Rhizoma had a large gap. The overall quality of P. forrestii, P. polyphylla and P. daliensis are better than that of P. polyphylla var. yunnanensis, and the comprehensive evaluation results of P. vietnamensis and P. polyphylla var. yunnanensis are closer that can be used as an alternative species for resource mining. Conclusion: The comprehensive evaluation of chemical quality has certain reference value for the quality evaluation of Paridis Rhizoma

7.
Acupuncture Research ; (6): 215-219, 2020.
Article in Chinese | WPRIM | ID: wpr-844187

ABSTRACT

OBJECTIVE: To observe the effect of moxibustion at "Zusanli"(ST36) on peripheral and central IL-6, IL-10 and IL-10/IL-6 changes in fatigue rats, so as to explore its mechanisms underlying relief of fatigue. METHODS: Twenty-one male SD rats were randomly divided into normal control, model and moxibustion groups with 7 rats in each group. The fatigue model was induced by exhausted weight-loaded swimming, once daily for 21 days. Moxibustion was applied to bilateral ST36 once every other day, for successive 11 times. The overall state and the body weight were observed. The levels of IL-6 and IL-10 in the serum, gastrocnemius and frontal cortex tissues were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared with the normal group, the body weight was significantly reduced on the 7th, 14th, and 21st day in the model group (P<0.05), and considerably increased on the 14th and 21st day in the moxibustion group relevant to the model group (P<0.05). After modeling, the levels of IL-6 in the serum, gastrocnemius and frontal cortex were significantly increased (P<0.05, P<0.01), while those of IL-10 was obviously decreased (P<0.05, P<0.01) in the model group relevant to the normal group, and ratio of IL-10/IL-6 was obviously decreased in the serum and frontal cortex of the model group relevant to the normal group (P<0.01). After the moxibustion intervention, the levels of IL-10 in the serum and frontal cortex were significantly increased (P<0.05), and ratio of IL-10/IL-6 in the serum and frontal cortex was significantly increased (P<0.01), while the IL-6 contents in the gastrocnemius and frontal cortex were notably decreased in the moxibustion group relevant to the model group (P<0.05). CONCLUSION: Moxibustion can inhibit weight-loaded swimming induced decrease of body weight in fatigue rats, which may be associated with its effects in balancing the levels of peripheral and central pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10.

8.
Article in English | WPRIM | ID: wpr-878346

ABSTRACT

Objective@#To explore the effects of prenatal exposure to polybrominated diphenyl ethers (PBDEs) on placental size and birth outcomes.@*Methods@#Based on the perspective Wenzhou Birth Cohort, this nested case-control study included 101 fetal growth restriction (FGR) and 101 healthy newborns. Maternal serum samples were collected during the third trimester and measured for PBDEs by gas chromatography tandem mass spectrometry. The basic information of mother-newborn pairs was collected from questionnaires, whereas the placental size and birth outcomes of newborns were obtained from hospital records.@*Results@#A total of 19 brominated diphenyle ether (BDE) congeners were detected in maternal serum samples. Higher concentrations of BDE-207, -208, -209, and ∑ @*Conclusion@#A negative association was found between PBDE levels in maternal serum and placental size and birth outcomes. Prenatal PBDE exposure may be associated with elevated risk of the incidence of FGR birth.


Subject(s)
Case-Control Studies , China/epidemiology , Female , Fetal Growth Retardation/epidemiology , Halogenated Diphenyl Ethers/blood , Humans , Incidence , Infant, Newborn , Placenta/physiology , Pregnancy , Risk Factors
9.
Article in Chinese | WPRIM | ID: wpr-773147

ABSTRACT

In the present study,non-targeted metabolomics technique was used to screen potentially susceptibility biomarkers in patients with mild liver function abnormalities during long-term use of Chinese herbal compound. According to the inclusion and exclusion criteria,we collected 7 cases of patients with abnormal liver function during the period of complete taking Chinese herbal medicine( 60 days),and 18 cases of patients with normal liver function in re-examination from the reproductive medicine center in our hospital. Ultra performance liquid chromatography coupled with time-of-flight mass spectrometry( UPLC-Q-TOF/MS~E) technique combined with Progenesis QI software was used to analyze the differential biomarkers in serum of patients with wild liver function abnormalities and normal liver function. 11 potential biomarkers such as bilirubin,pantothenic acid,hippuric acid,sphingomyelin,palmitic acid,and oleic acid were tentatively identified. Metabolic disorders in patients with herbal-induced mild liver abnormality were mainly related to two pathways: pantothenic acid and coenzyme A biosynthesis and linoleic acid metabolism. It could provide a reference for the early warning of mild liver function abnormalities of patients that may be caused by long-term use of Chinese medicine compound in clinical application,and will lay a foundation for further understanding the endogenous substance changes in different levels of liver injury.


Subject(s)
Biomarkers , Blood , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Therapeutic Uses , Humans , Liver Diseases , Blood , Mass Spectrometry , Metabolomics
10.
Article in Chinese | WPRIM | ID: wpr-802330

ABSTRACT

Objective:To analyze and determine heavy metal content in Paridis Rhizoma from different genus and localities,in order to provide a reference for selecting cultivation areas and establishing the quality standard of Paridis Rhizoma of heavy metals content. Method:Microwave digestion method combined with inductively coupled plasma atomic emission spectrometry method (ICP-AES) method were applied to determine the contents of 6 heavy metals,i.e. As,Cu,Hg,Cd,Pb and Cr in 39 samples of Paridis Rhizoma of different genus and localities in Yunnan Province. Cluster analysis,statistical analysis and principal component analysis (PCA) were used to compare the differences of heavy metals contents in different localities and species. Result:The contents of six heavy metals in Paridis Rhizoma met the ISO international standard of Heavy Metal Limit of Traditional Chinese Medicine-traditional Chinese Medicinal Materials. Under the limited value standard of Green Trade Standards of Importing Medicinal Plants and Preparations,the over-standard rate of heavy metal As was 15.4%,the excess rate of Cd was 5.1%,and the excess rate of Pb was 2.6%. The contents of Cu and Hg conformed to relevant requirements. Cluster analysis,statistical analysis and principal component analysis showed that for the same variety,differences in producing places had significant effects on heavy metal content,while differences in species had little effects. Conclusion:The results of this study indicated that the heavy metal content of Paris planted in and around Dali basically conformed to relevant standards. The differences of heavy metal content in Paris were mainly regional differences,which provided a theoretical basis for standardizing the cultivation of medicinal materials and formulating the limit standards of heavy metals for Paridis Rhizoma.

11.
Article in Chinese | WPRIM | ID: wpr-802328

ABSTRACT

Genus Polygonatum,belonging to the Liliaceae family,is mostly medicine food homology species,which have been documented as treating " dry mouth,less food,and internal heat and thirst" in medical ancient books. In traditional Chinese medicine clinic,it was used to treat diabetes,hyperlipemia and asa rehabilitation therapy of cancer. With deepening the basic research and industrial development of this genus,the bioactive component groups and their action mechanisms of prevention and treatment of diabetes and diabetic complication continue to be revealed. By consulting ancient Chinese herbal medicine and modern literature,the research on the herbology,material basis and mechanism of the genus Polygonatum were reviewed. The result indicated that the plants of Polygonatum were used for the treatment of diabetes,based on the ancient herbal literature and modern research,the polysaccharides,flavonoids and saponins were their active ingredients. Among them,the significant chemical constituents of decreasing blood glucose were Polygonatum polysaccharides,and the flavonoids and saponins also have this function. Although some scholars have studied the anti-diabetic efficacy and material basis of genus of Polygonatum,the pharmacodynamics,mechanism and metabolic pathways are not clear. Therefore,further strengthening of the related research of genus of Polygonatum,especially use of modern scientific and technological research to clarify the material basis of its efficacy,and mechanisms,are crucial to accelerate the modernization and development of anti-diabetic drugs and utilization of genus of Polygonatum.

12.
Article in Chinese | WPRIM | ID: wpr-802007

ABSTRACT

Objective: To optimize purification process of total alkaloid extract of Berberis dictyophylla cortex by macroporous resin,and to establish its quality standard. Method: Acid dye colorimetry was used to investigate the purification process of total alkaloid extract of B. dictyophylla cortex,the process parameters included concentration of sample solution,speed of sampling,diameter-height ratio of resin column,water washing amount,concentration and dosage of eluent,flow rate of elution,etc.In order to determine the optimum process,HPLC was employed to determine the contents of four alkaloids(magnoflorine,jatrorrhizine hydrochloride,palmatine hydrochloride,and berberine hydrochloride) with mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution for gradient elution and detection wavelength at 270 nm.After being purified,quality standard of total alkaloid extract of B. dictyophylla cortex was investigated according to the requirements in the 2015 edition of Chinese Pharmacopoeia. Result: Optimal purification conditions were as following:10 g of HPD100 macroporous adsorption resin with a column diameter-height ratio of 1:8,sampling solution concentration of 11 g·L-1,the loading flow rate of 1 mL·min-1,sampling solution volume of 50 mL,washed with 4 BV of water(1 BV=15 mL) and added 9 BV of 30% ethanol,after being purified,the transfer rate of total alkaloids was>80%,and its purity was>65%.The quality standard of total alkaloid extract of B. dictyophylla cortex was established,there were 19 common peaks in the characteristic chromatogram,and the overall similarity was>0.99. Conclusion: This optimized purification process is stable and feasible, and the established quality standard is controllable.

13.
Article in Chinese | WPRIM | ID: wpr-801971

ABSTRACT

Objective: To investigate the effect of berbamine hydrochloride on the absorption characteristics of berberine hydrochloride in different intestinal segments of rats in normal environment and high calcium environment. Method: Taking rat everted intestinal sac model,the content of berberine hydrochloride in absorbent solution of everted intestinal sac from different compatibility groups was determined by HPLC,and the uptake per unit area in different groups was analyzed by One-way ANOVA. Result: Compared with the normal J70 group(in normal environment,the concentration of berberine hydrochloride was 70 mg·L-1) at the same time point,the uptake per unit area of the normal J70+Ver100 group(in normal environment,the concentration of berberine hydrochloride was 70 mg·L-1,adding verapamil hydrochloride to a concentration of 100 mg·L-1) was significantly increased in the ileum(P-1,adding berbamine hydrochloride to a concentration of 35 mg·L-1) were significantly increased in the duodenum(P-1,adding berbamine hydrochloride to a concentration of 70 mg·L-1) were significantly increased in the ileum(PConclusion: Berbamine hydrochloride can promote the absorption of berberine hydrochloride in intestine to a certain extent,especially in the high calcium environment.

14.
Article in Chinese | WPRIM | ID: wpr-798370

ABSTRACT

Diabetic nephropathy(DN) is a kind of severe diabetes mellitus(DM) complications,which is one of the most common cause of end-stage renal failure.The incidence of DN increases with the increase of the number of DM patients,and early prevention is the key.DM and DN are characteristic advantages of Tibetan medicine,the "holistic view" of dynamic balance based on the three major factors in the body(Sanyin,included rlung,mkhris-pa and bad-gan) is a unique understanding of DM and DN among Tibetan medical practitioners,and which has rich and unique clinical and medication experiences in DN treatment.This paper discussed the medical theory "holistic view" on the Tibetan medicine for DM and DN,dug commonly used Tibetan drugs and Tibetan medical prescriptions for clinical treatment of DN,in order to provide new ideas for the treatment of DN and the discovery of new drugs for DN, and give full play to the unique advantages of Tibetan medicine.

15.
Article in Chinese | WPRIM | ID: wpr-843406

ABSTRACT

Objective: To analyze the risk factors of hyperuricemia (HUA) and its outcomes of renal function in patients with diabetes mellitus in Shanghai. Methods: A total of 3 454 patients with diabetes who were admitted to the Shanghai Diabetes Clinical Center from July 2011 to September 2014 were selected. The prevalence of HUA, predisposing factors and renal function of diabetic patients were analyzed. Patients were divided into HUA group (n=548) and normal serum uric acid (SUA) group (n=2 906). General characteristics and clinical parameters of diabetic patients were compared between two groups. Pearson correlation analysis and multivariate Logistic regression were used to analyze the relationship between SUA and renal dysfunction and abnormal proteinuria in diabetic patients. Results: In Shanghai patients with diabetes, the prevalence of HUA was 15.87%, 14.52% in men, 17.80% in women. Glomerular filtration rate (GFR), fasting C-peptide, gender, age, natural logarithm of the ratio of microalbuminuria to creatinine (LnACR), glycated albumin (GA), triacylglycerol (TAG), high density lipoprotein cholesterol (HDL-C) and body mass index (BMI) were independently associated with SUA (all P<0.05). In diabetic patients with HUA, 1-SD increment in the SUA level was associated with a 0.8% increased prevalence of renal dysfunction, 0.3% increased prevalence of incident abnormal albuminuria and 0.5% decreased prevalence of hyperfiltration (all P<0.05). Conclusion: Diabetes has an important impact on HUA. Actively improving insulin resistance and controlling blood glucose may improve HUA. HUA is an independent risk factor for the occurrence and development of diabetic nephropathy. Therefore, monitoring the level of SUA in diabetic patients is of great significance in the prevention of diabetic nephropathy.

16.
Article in Chinese | WPRIM | ID: wpr-837898

ABSTRACT

Objective To explore the effect of aerobic exercise on blood lipid of hyperlipidemia rats and its mechanism. Methods A total of 120 healthy male SD rats aged 8 weeks were randomized into normal control group, high-fat diet (HF) group, SBC-115076 group and aerobic exercise group, with 30 rats in each group. The rats in the normal control group were fed with standard diet, and the rats in the other 3 groups were fed with HF to establish hyperlipidemia rat model. The rats in the SBC-115076 group were injected with proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor SBC-115076 (8 mg/kg) once a week for 8 weeks, and the rats in the aerobic exercise group underwent swimming without load 6 days a week for 8 weeks. After 8 weeks, the rats were sacrificed and the blood samples were collected to determine the levels of serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Pathological changes of thoracic aorta were observed using H-E staining. The mRNA and protein expression levels of PCSK9, low-density lipoprotein receptor (LDLR) and sterol regulatory element binding protein (SREBP) in the hepatic tissues were detected by quantitative real-time PCR, Western blotting and immunofluorescence. Results Compared with the normal control group, the levels of serum TG, TC and LDL of the rats were significantly higher in the HF group, and the level of HDL was significantly lower (P<0.01). Compared with the HF group, the levels of serum TG, TC and LDL of the rats were significantly lower in the SBC-115076 and aerobic exercise groups, and the level of HDL was significantly higher (P<0.01). In the HF rats, the aortic tunica intima was thickened and endothelial cells were damaged and exfoliated. Compared with the HF group, the aortic intima thickening was reduced and endothelial damage was less in the aerobic exercise group. Compared with the normal control group, the mRNA and protein expression levels of PCSK9, SREBP1 and SREBP2 were significantly higher in the HF group, and the mRNA and protein expression levels of LDLR were significantly lower (P<0.01). Compared with the HF group, the mRNA and protein expression levels of PCSK9, SREBP1 and SREBP2 were significantly lower, and the mRNA and protein expression levels of LDLR were significantly higher (P<0.01). Conclusion Aerobic exercise can down-regulate the expression of TG, TC and LDL, up-regulate the expression of HDL, and alleviate the intimal thickening of aorta. The mechanism may be related to down-regulating the expression of PCSK9 and SREBPs, thus eliminating the inhibition of LDLR.

17.
Article in Chinese | WPRIM | ID: wpr-851381

ABSTRACT

Objective: To explore morphology and infrared spectrum identification evidence of Paris polyphylla var. yunnanensis and its closely relative species, and further analyze their genetic relationship to provide basis for the development and utilization of medicinal plant resources of genus Paris. Methods: The morphology and infrared spectrum of P. polyphylla var. yunnanensis and its closely relative species were studied systematically and compared with each other. The original infrared spectra data were pretreated by automatic baseline correction, automatic smoothing, ordinate normalization, and second derivative, and analyzed by principal component analysis (PCA), partial least squares discrimination analysis (PLS-DA), and hierarchical cluster analysis (HCA). Results: There were typical characteristics of P. polyphylla var. yunnanensis and its closely relative species, such as plant size, leaves, veins, sepals, petals, flower pavilions and stamens, which could provide morphological identification evidence. There were obviously differences of the second derivative in 3 000-2 000 cm-1 and the fingerprint in 1 800-500 cm-1 in the six types of genus Paris. Both HCA and PLS-DA could better distinguish Paris polyphylla var. yunnanensis and its closely relative species, which could provide an infrared spectral identified evidence. The Results: of HCA showed that P. polyphylla var. yunnanensis, P. polyphylla var. chinensis, P. polyphylla, P. polyphylla var. stenophylla and P. polyphylla var. nana were relatively close. Conclusion: Fourier transform infrared spectroscopy (FTIR) combined with original plant morphological identification can quickly identify P. polyphylla var. yunnanensis and its closely relative species, which will provide a scientific basis for the cultivation, clinical application, and resource development of genus Paris.

18.
Journal of Chinese Physician ; (12): 521-524,531, 2019.
Article in Chinese | WPRIM | ID: wpr-744903

ABSTRACT

Objective To observe aerobic exercise on blood lipid and intercellular adhesion molecule-1 (ICAM-1),lymphocyte function-associated antigen-1 (LFA-1) in hyperlipidemia rats.Methods 120 male Sprague Dawley (SD) rats were randomized into the 4 groups (n =30):normal diet group (control group),high fat group (HF group),HF + SBC-115076 group [SBC-115076,8 mg/kg · w)] and HF + aerobic exercise group.Rats in HF group and HF + SBC-115076 group did not exercise during feeding and lived in normal cage.Rats in HF + SBC-115076 group recieved PCSK9 inhibitor SBC-115076 (8 mg/kg) injection once a week for 8 weeks.HF + aerobic exercise group underwent load-free swimming training 6 days a week for 8 weeks.The rats were sacrificed 8 weeks later.The serum levels of triglyceride (TG),total cholesterol (TC),low density lipoprotein (LDL),high density lipoprotein (HDL),ICAM-1 and LFA-1 were measured by apical blood sampling.Hematoxylin-eosin (HE) staining of thoracic aorta to observe pathological changes of aorta.Results After modeling,there was no significant difference in the food intake levels of the hyperlipidemia rats (P > 0.05).The body weight of HF rats and HF + SBC-115076 rats increased significantly than HF + aerobic exercise rats (P < 0.01).The levels of serum lipid profile,ICAM-1,and LFA-1 were significantly different between HF rats and control rats.Compared with HF rats,serum levels of TG,TC,LDL,ICAM-1 and LFA-1 in HF + SBC-115076 group and HF + aerobic exercise group were significantly lower and HDL levels were significantly higher.HE staining showed that the intimal thickening was significantly improved in HF + aerobic exercise group with less endothelial damage.Conclusions Aerobic exercise can reduce the levels of serum TG,TC,LDL and increase HDL level in rats with hyperlipidemia.It can improve the intimal thickening and endothelial damage caused by high-fat diet by reducing the expression levels of serum ICAM-1 and LFA-1,and has anti-atherosclerosis effect.

19.
Article in Chinese | WPRIM | ID: wpr-693720

ABSTRACT

Objective To investigate the clinical efficacy of staging treatment with various acupuncture therapies mainly on Yifeng (TE17) point for the treatment of peripheral facial paralysis. Methods One hundred and two peripheral facial paralysis patients were randomly divided into trial group and control group, 51 patients in each group. The trial group was given acupuncture mainly on Yifeng point, by Yang needling(plum-blossom needling) for acute stage, Qi needling(concentrated triple-needling) for resting stage and Bang needling(lateral needling) for recovery stage. The control group was given conventional acupuncture. The treatment for the two groups was performed once a day, 5 continuous days constituted a course, and the treatment lasted 3 courses. Before treatment and after treatment for 1, 2, 3 course (s), facial nerve function scores were evaluated with House-Brackmann Grading Scale (HB) and the scores of pain in head and face were evaluated with Visual Analogue Scale (VAS). And the clinical efficacy of the two groups was assessed after treatment. Results (1) After treatment for 3 courses, the cure rate and total effective rate of the trial group were 82.35%, 100.00%, and those of the control group were 60.78%, 86.27%, respectively. The differences between the two groups were significant (P <0.05). (2) After treatment, HB scores and VSA scores of patients with facial pain and headache in the two groups were improved after treatment (P < 0.05 compared with those before treatment), and the improvement in the trial group was superior to that in the control group (P < 0.05). Conclusion Staging treatment with various acupuncture therapies mainly on Yifeng point is more effective for the treatment of peripheral facial paralysis than conventional acupuncture.

20.
Article in Chinese | WPRIM | ID: wpr-665332

ABSTRACT

Objective To analyze the interaction between family history and waist circumference on incidence of hypertension .Methods We conducted a cross-sectional survey of rural residents in Hanzhong .Questionnaires about hypertension were collected and physical examination was conducted .A total of 3030 rural residents' data were collected . Logistic regression model was used to analyze the influences of family history and waist circumference on hypertension . Additive model and multiplicative model were used to analyze the interaction between family history and waist circumference on hypertension .Results For hypertension , Logistic regression analysis showed that family history (OR :2 .225 ,95% CI:1 .843-2 .687) and abdominal obesity (OR :1 .976 ,95%CI :1 .649-2 .368) were positively associated after adjustment for age ,sex ,salt intake ,smoking ,and alcohol drinking .Family history and waist circumference had no interaction on incidence of hypertension on a multiplicative scale but an interaction on an additive scale .The relative excess risk due to interaction (RERI) was 1 .848 (95% CI :0 .693-3 .002) ,the attributable proportion due to interaction (AP) was 0 .404 (95% CI :0 .227 -0 .582) and the synergy index (S) was 2 .073 (95% CI :1 .340 -3 .206 ) .Conclusion Family history and abdominal obesity are risk factors for hypertension . The incidence of hypertension of people with both family history and abdominal obesity is obviously higher than the sum of the two risk factors separately .Therefore ,to prevent hypertension it is more important for people to control their weight when they have a positive family history .

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