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1.
Clinical Medicine of China ; (12): 481-488, 2021.
Article in Chinese | WPRIM | ID: wpr-909782

ABSTRACT

Objective:To study the clinical prognosis and related factors affecting optimal medical therapy (OMT) compliance of patients with coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI).Methods:A prospective study was conducted to select 3 818 patients who were diagnosed with CAD and successfully underwent PCI in TEDA International Cardiovascular Hospital from October 2016 to September 2017. The clinical information and application of OMT during hospitalization and 1 year later were collected for research.The patients were divided into OMT group and non OMT group according to whether they adhered to OMT during follow-up one year after discharge. After comparing the imbalance baseline data of hypertension,diabetes and hyperlipidemia with propensity score,demographic characteristics, coronary revascularization history, CAD, laboratory related laboratory examinations,and the use of OMT drugs were compared between the two groups. Cox regression model was used to analyze the relationship between long-term OMT and clinical prognosis in patients with CAD.Multivariate binary logistic regression was used to analyze the related factors affecting long-term OMT compliance.Results:A total of 3 818 cases of CAD patients were matched by propensity score and 2 596 patients were included in the study. There were 1 609 males and 987 females. The age was (62.51±9.56) years old.One year later,1298 patients (50%) insisted on OMT,including dual antiplatelet therapy(DAPT), statins, β-blockers and ACEI/ARB were 97.0% (2 517/2 596),94.5%(2 454/2 596),69.6% (1 806/2 596) and 64.2% (1 666/2 596), especially angiotensin converting enzyme inhibitors / angiotensin receptor blockers and β Receptor blockers decreased the most.Cox regression analysis showed that after adjusting for other factors, compared with non-adherence to OMT group,OMT after PCI was associated with better prognosis ( HR=0.416,95% CI 0.270-0.641, P<0.001). The prognosis of CAD patients with history of old myocardial infarction ( HR=1.804,95% CI 1.070-3.041, P=0.027),cardiac insufficiency ( HR=2.074,95% CI 1.161-3.702, P=0.014),multivessel coronary disease ( HR=2.211,95% CI 1.228-3.983, P=0.008) and BMI>24 ( HR=1.570,95% CI 1.037-2.377, P=0.033) were related to worse clinical outcomes. Multi-factor binary Logistic regression showed that OMT at hospitalization was a strong influencing factor of long-term adherence to OMT ( OR=41.278,95% CI 29.961-56.871, P<0.001). Patients with higher education,employee medical insurance and with history of PCI tend to persist in OMT. Conclusion:The medication compliance of patients with long-term OMT after PCI is still poor,while the high compliance of OMT is related to the lower incidence of adverse cardiovascular events,including death, nonfatal myocardial infarction and stroke. If there is no obvious contraindication,all patients after PCI should adhere to OMT.

2.
Article in Chinese | WPRIM | ID: wpr-908693

ABSTRACT

Objective:To explore the molecular mechanism of carbapenem antibiotic resistance in burns patients infected with Klebsiella pneumoniae.Methods:The patients who were diagnosed with Klebsiella pneumoniae in the burns department of Taizhou Central Hospital (Affiliated Hospital of Taizhou University) from May 2018 to October 2019 were enrolled.Ten carbapenem-resistant Klebsiella pneumoniae (CR-KP) bacterium were isolated, then the degree of resistance of CR-KP bacteria was analyzed.The common resistance genes was detected by polymerase chain reaction (PCR), the existence of resistance genes was verified through plasmid conjugation tests, and the expression of OmpK36 protein in the membrane pores was analyzed.Results:The 10 strains in this study all showed different degrees of drug resistance. The results of PCR showed that all drug resistant bacteria carried the NDM gene, and 4 strains carried the KPC gene. In addition, only a small number of resistant bacteria carried extended-spectrum β-lactamase (ESBLs) genes and AmpC genes. Plasmid conjugation experiments showed that most of the Zygomycetes were resistant to carbapenem antibiotics, and the Western blot results indicated that the protein expression of OMPK36 in the membrane pore of Zygomycetes was decreased.Conclusions:The resistance of CR-KP bacteria in burns patients was mainly caused by plasmid-mediated NDM gene insertion and reduced expression of OMPK36 protein in the membrane pore.

3.
Journal of Leukemia & Lymphoma ; (12): 461-465, 2021.
Article in Chinese | WPRIM | ID: wpr-907199

ABSTRACT

Objective:To investigate the significance of Th1/Th2 cytokines in prognostic stratification of acute myeloid leukemia (AML).Methods:A total of 83 patients with newly diagnosed AML from June 2017 to April 2019 in the First People's Hospital of Yunnan Province were collected. According to the Chinese guidelines for diagnosis and treatment of adult acute myeloid leukemia (non-acute promyelocytic leukemia) (2017 edition), AML patients were divided into poor prognosis group (45 cases), moderate prognosis group (19 cases), and good prognosis group (19 cases); moderate prognosis plus poor prognosis was treated as the not good prognosis. Mann-Whitney U test and Kruskal-Wallis H test were used to compare the expression differences of Th1/Th2 cytokines in peripheral blood of different prognosis groups; cytokines with statistical differences among different prognosis groups were selected, and the cut-off value of AML patients with different prognostic stratification distinguished by cytokines was determined by using receiver operating characteristic (ROC) curve. Finally, patients were divided into ≥ cut-off value group and <cut-off value group according to the cut-off value, and then the association of both groups with the prognostic stratification in guideline was also analyzed. Results:The median expression level of tumor necrosis factor (TNF)-β of patients in moderate prognosis group [3.80 pg/ml (2.75 pg/ml, 15.32 pg/ml)] was higher than that of patients in poor prognosis group [2.78 pg/ml (1.28 pg/ml, 3.36 pg/ml)] and good prognosis group [1.61 pg/ml (0.83 pg/ml, 3.04 pg/ml)] ( U=216, P=0.02; U = 312, P < 0.05); the median expression level of TNF-β in good prognosis group was lower than that in poor prognosis group ( U = 562, P = 0.048). There were no statistically significant differences in the expression levels of Th1/Th2 cytokines of AML patients with different prognostic stratification (all P>0.05).The cut-off value of TNF-β was 3.23 pg/ml in good prognosis group and moderate prognosis group, the area under the ROC curve was 0.866 (95% CI 0.753-0.978, P < 0.05); among 26 patients with TNF-β≥ 3.23 pg/ml, 25 (96.2%) patients had not good prognosis. The cut-off value was 3.62 pg/ml for distinguishing between moderate prognosis group and poor prognosis group, the area under the ROC curve was 0.747 (95% CI 0.610-0.884, P = 0.02); 18 (100%) patients with TNF-β≥ 3.62 pg/ml had not good prognosis. The cut-off value was 2.19 pg/ml for distinguishing between good prognosis group and not good prognosis group, the area under the ROC curve was 0.719 (95% CI 0.595-0.842, P = 0.04); among 53 patients with TNF-β≥2.19 pg/ml, 46 (86.8%) patients had not good prognosis. Conclusions:The high expression of TNF-β may indicate that the prognosis of AML patients is not good. When the level of TNF-β was equal or greater than 3.62 pg/ml, it may contribute to the prognostic stratification of AML patients.

4.
Chinese Journal of Radiology ; (12): 763-768, 2020.
Article in Chinese | WPRIM | ID: wpr-868341

ABSTRACT

Objective:To verify the ability of the newly developed ultra-high resolution CT (U-HRCT) for displaying the fine bony anatomy of temporal bone.Methods:The cone-beam CT architecture was used on U-HRCT. The focus size of X-ray generator was 0.27 mm × 0.29 mm, the rated tube voltage was 60-100 kV, and the unit size of flat panel detector was 0.074 8 mm×0.074 8 mm. From October to December 2019, 16 adult head specimens (32 temporal bones, provided by Huanghe University of Science and Technology) were imaged using U-HRCT. The scanning mode was small-field-of-view and high-resolution scanning for unilateral temporal bone. The scanning parameters were: voltage 100 kV, current 3.5 mA, rotation time 40 s, reconstructed field of view 65 mm×65 mm, voxel size 0.1 mm×0.1 mm×0.1 mm, layer number 370, slice thickness 0.1 mm, and slice interval 0.1 mm. The ultimate spatial resolution of the system was detected using a phantom with line pair card, after the scanning with the same parameters. Through multi-planar reconstruction and minimum intensity projection method, a total of 6 anatomical positions of 4 structures (stapes footplate, cochlear axis bottom, vestibular aqueduct internal orifice and isthmus, cochlear aqueduct internal orifice and auditory sac segment) were scored with 1-3 points. The Wilcoxon test was used to compare the difference of bilateral scores of each structure.Results:The phantom test results showed that the ultimate spatial resolution of the system was ≥4.0 lp/mm. The scores of stapes footplate, cochlear axis bottom and vestibular aqueduct internal orifice were ≥2 points, with a display rate of 100%. The display rates of vestibular aqueduct isthmus, cochlear aqueduct internal orifice and auditory sac segment were 87.5% (28/32), 71.9% (23/32) and 53.1% (17/32), respectively. There were no significant differences in left and right scores of all anatomical structures ( P>0.05). Conclusion:The newly developed U-HRCT has good display ability for fine bony anatomy of temporal bone and has great clinical application potential.

5.
Article in Chinese | WPRIM | ID: wpr-866674

ABSTRACT

Objective:To investigate the levels of trace elements(iron, zinc, magnesium and copper) in 0~9 years old children in Shenyang, and to explore the relationship between trace elements and humoral immunity(IgA, IgG and IgM).Methods:From September 2018 to October 2019, retrospective analysis was performed in children aged 0-9 who admitted to the Fourth Hospital Affiliated to China Medical University and underwent trace elements and humoral immunity(IgA, IgG and IgM).Results:A total of 422 children met the criteria, including 228 boys and 194 girls.The rates of iron deficiency in children aged 0-3, 4-6 and 7-9 were 17.5%, 18.2% and 14.0%, respectively, and zinc deficiency rates were 10.0%, 9.0% and 10.5%, respectively.There were no statistically significant differences in the rates of iron deficiency and zinc deficiency in each age group(χ 2=0.520, P>0.05; χ 2=0.135, P>0.05). Magnesium and copper levels in all children were in the normal range.The levels of IgA and IgG [(1.24±0.77)g/L, (9.07±3.04)g/L] in the zinc deficiency group were significantly lower than those in the normal group [(0.94±0.55)g/L, (8.18±2.11)g/L], the differences were statistically significant( t=-2.858, -2.440, all P<0.05), while there was no statistically significant difference in IgM level between the two groups( t=-0.870, P>0.05). There were no statistically significant differences in IgA, IgG and IgM levels between the iron deficiency group and the iron normal group( t=-1.346, -0.960, 0.029, all P>0.05). There was a significant positive correlation between zinc level and IgA, IgG levels( r=0.184, 0.142, all P<0.05), but there was no significant correlation with IgM( r=0.08, P>0.05). Iron, copper and magnesium levels were not significantly correlated with IgA, IgG and IgM levels( P>0.05). Conclusion:Deficiency of zinc can cause reduction of IgA and IgG, which may be related to repeated infection in children.

6.
Article in Chinese | WPRIM | ID: wpr-863455

ABSTRACT

Triple negative breast cancer (TNBC) is associated with a high risk of recurrence and generally a poor prognosis. Median survival time of metastatic triple negative breast cancer (mTNBC) is about one year. Currently, the mainstay of treatment for mTNBC remains cytotoxic chemotherapy. In recent years, the drugs involved in the clinical researches of mTNBC include poly-ADP-ribose polymerase inhibitors, phosphoinositide 3-kinase pathway inhibitors, antibody targeting epidermal growth factor receptor, antibody targeting trophoblast surface antigen-2, antibody targeting death receptor 5, endocrine therapy drugs for androgen receptor, and immune checkpoint inhibitors and so on. New drugs have gradually improved the survival of patients with mTNBC.

7.
Journal of Leukemia & Lymphoma ; (12): 637-640, 2020.
Article in Chinese | WPRIM | ID: wpr-862885

ABSTRACT

The intestinal flora in patients with leukemia is significantly different from healthy people. Some studies have found that intestinal flora can participate in leukemia progression by regulating the body's immune system and hematopoietic system and affecting the metabolic function, but the underlying molecular mechanism is not clear. Maintaining the intestinal microecological balance and exploring intestinal microbes that can be used as therapeutic targets have a profound significance for prolonging the survival of leukemia patients.

8.
Journal of Leukemia & Lymphoma ; (12): 566-569, 2020.
Article in Chinese | WPRIM | ID: wpr-862877

ABSTRACT

At present, the treatment methods of lymphoma mainly include the combined chemotherapy, hematopoietic stem cell transplantation, immunotherapy and new targeted therapies, but the treatment-related drug resistance, recurrence, extranodal and central nervous system infiltration, and leukemia transformation are still intractable problems that need to be solved in clinical practice. Studies have shown that cytokines are expressed to varying degrees in patients with lymphoma, which are significantly related to the progression of lymphoma, poor prognosis, chemotherapy response, and drug resistance. It has been confirmed that interleukin 6 (IL-6) and IL-10 are highly expressed in all types of lymphoma, and IL-10 is highly expressed in the cerebrospinal fluid of central nervous system lymphoma, all of which indicate a poor prognosis. This article reviews the role of cytokines in the development, treatment and prognosis of lymphoma.

9.
Chinese Journal of Biotechnology ; (12): 1679-1688, 2020.
Article in Chinese | WPRIM | ID: wpr-826809

ABSTRACT

Based on the rDNA sequence of Pichia pastoris, a multi-copy gene expression vector of transglutaminase (pPICZα-rDNA-mtg) was constructed and transformed to the host strain (pGAP9-pro/GS115) expressing pro peptide, to obtain the co-expression strain pro/rDNA-mtg (GS115). Real-time fluorescence quantitative PCR (qPCR) was used to analyze transglutaminase gene copy number in the 4 positive strains. We further studied the effect of gene copy on the enzyme production of recombinant Pichia pastoris as well as high-density fermentation of higher expression strain in a 3-L fermenter. The mtg copy numbers of the 4 positive strains were 2.21, 3.36, 5.72 and 7.62 (mtg-2c, mtg-3c, mtg-6c and mtg-8c), respectively, and the enzyme production capacity and protein expression level were mtg-3c>mtg-2c>mtg-6c>mtg-8c. Mtg-3c and mtg-6c of high-density fermentation had the highest enzymatic activity and enzymatic activity per unit wet weight in the supernatant of 3.12 U/mL, 52.1 U/g (wet weight) and 2.07 U/mL and 36.5 U/g (wet weight), respectively. In terms of enzyme activity per unit wet weight, mtg-3c is 1.4 times higher than that of mtg-6c. The activity of purified enzyme (mtg-3c) was up to 7.21 U/mL and the protein concentration was 437.2 μg/mL. By analyzing the effect of mtg copy number on the enzyme production of recombinant strains, mtg-3c is suitable for the co-expression of two genes (pro and mtg) in pro/rDNA-mtg, and its enzyme activity is related to higher protein secretion of the strain.

10.
China Pharmacy ; (12): 2392-2398, 2020.
Article in Chinese | WPRIM | ID: wpr-825897

ABSTRACT

OBJECTIVE:To analyze th e general characteristics and application of the models used in the pharmacoeconomic evaluation of type 2 diabetes mellitus (T2DM)therapy during the past 5 years,and to provide reference for the selection and improvement of T 2DM pharmacoeconomic model. METHODS :Retrieved from PubMed ,Embase,CNKI,Wanfang database and VIP during Jan. 1st,2015 to Dec. 31st,2019,pharmacoeconomic evaluation literatures about T 2DM therapy were collected ;the included model was analyzed in respects of general structure ,therapy plan establishment , short-term therapeutic efficacy , complication simulation ,model effectiveness validation ,application frequency. RESULTS :A total of 81 literatures were included , involving 14 models,such as CORE model ,Cardiff model ,ECHO model ,etc. Mostly ,Markov or micro Markov simulation method were adopted to measure the patient ’s lifetime health outcome and cost mostly from the point of view of third-party payer. Seven models could simulate 2-4 therapy plans ;short-term efficacy mainly included risk factors of diabetic complications (such as glycosylated hemoglobin level and body mass )and adverse drug reactions. Most models used intermediate indexes to simulate the occurrence of complications ,and the number of complications ranged from 3 to 15;the validity of model effectiveness included surface validity ,internal validity and external validity ,etc. Among 14 models,the most frequently used models in the past 5 years were long-term models that had been validated ,among which CORE model had the most application times (38/81,46.91%), followed by Cardiff model (12/81,14.81%). CONCLUSIONS :The 14 models have similar structure. The differences of the models are mainly reflected in 3 aspects as therapy plan setting, considered short-term efficacy , the number of model are ideally choose based on available evidences.

11.
Chinese Journal of Dermatology ; (12): 880-885, 2020.
Article in Chinese | WPRIM | ID: wpr-870376

ABSTRACT

Objective:To investigate the expression of epigenetic inhibitor polycomb group proteins such as enhancer of zeste homolog 1/2 (EZH1/EZH2), embryonic ectoderm development protein (EED) and suppressor of zeste 12 (SUZ12) in common cutaneous T-cell lymphomas and lymphoproliferative disorders (CTCL/LPD) .Methods:Totally, 93 paraffin-embedded skin samples of CTCL/LPD and 8 of lichen planus were collected from Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College between 2012 and 2019, and subjected to immunohistochemical staining to determine the protein expression of EZH2, EED, SUZ12 and EZH1. Statistical analysis was carried out with SPSS 25.0 software by using chi-square test and Spearman correlation analysis.Results:The 93 cases of CTCL/LPD included 44 cases of mycosis fungoides (MF), 17 natural killer/T cell lymphoma (NK/TCL), 8 primary cutaneous anaplastic large cell lymphoma (PC-ALCL), 8 lymphomatoid papulosis (LyP), 8 hydroa vacciniforme-like lymphoproliferative disorder (HV-like LPD) and 8 cases of subcutaneous panniculitis-like T cell lymphoma (SPTCL). Among the 93 CTCL/LPD cases, 83 (89.2%) were positive for EZH2, 81 (87.1%) for EED, 78 (83.9%) for SUZ12 and 37 (39.8%) for EZH1; among the 8 cases of lichen planus, 1 was positive for EZH2, all were positive for EZH1, and all were negative for EED and SUZ12. The expression of EZH2, EED, SUZ12 and EZH1 in lichen planus samples significantly differed from all the CTCL/LPD samples ( χ2 = 41.75, 39.74, 39.36, 32.83, respectively, all P < 0.001), and from MF, NK/TCL, PC-ALCL, LyP, HV-like LPD and SPTCL samples separately (α = 0.008 3, all P < 0.001). Meanwhile, the score of EZH2 expression was negatively correlated with that of EZH1 expression in the MF, NK/TCL, PC-ALCL, LyP, HV-like LPD and SPTCL tissues ( rs = -0.60, -0.68, -0.89, -0.74, -0.93, -0.80, respectively, all P < 0.05) . Conclusion:Polycomb group proteins EZH2, EED, SUZ12 and EZH1 are abnormally expressed in CTCL/LPD lesions.

12.
Article in Chinese | WPRIM | ID: wpr-754667

ABSTRACT

Objective To study the detection rate of pathogens from sputum , blood, and bronchoalveolar lavage fluid ( BALF ) samples in acquired immunodeficiency syndrome ( AIDS ) patients complicated with pulmonary infection.Methods Seventy-three hospitalized AIDS patients complicated with pulmonary infection in Beijing Ditan Hospital , Capital Medical University were enrolled from February 2018 to September 2018.Blood, sputum and BALF samples were collected.Blood samples were cultured to detect anaerobic bacteria, aerobic bacteria, fungi and mycobacteria.Antigen agglutination method was applied in blood samples to detect cryptococcus neoformans.The sputum samples were tested for Mycobacterium tuberculosis by acid-fast staining and were cultured to detect bacteria and fungi.The sputum samples were observed under microscope for sporotrichosis and fungal spores.The BALF samples were cultured to detect bacteria and fungi. The BALF samples were tested for Mycobacterium tuberculosis by polymerase chain reaction amplification and acid-fast staining.Pneumocystis were detected in BALF samples by methenamine silver staining method .The BALF samples were observed under a microscope for sporotrichosis and fungal spores .The detection rate of pathogens from blood, sputum and BALF samples were compared.Chi-square test was conducted for statistical analysis.Results In 73 AIDS patients complicated with pulmonary infection , the pathogen detection rates in blood, sputum and BALF samples were 8 (11.0%), 23 ( 31.5%) and 48 (65.8%), respectively.The difference was statistically significant ( F =48.513, P <0.01 ).The detection rate in BALF samples was significantly higher than that in blood or sputum samples ( χ2 =43.349 and 17.136, respectively, both P<0.01).The detection rate in sputum samples was significantly higher than that in blood (χ2 =9.215, P<0.05). The highest detection rates of pathogens in blood , sputum and BALF samples were Talaromyces marneffei 4.1%(3), viridans group streptococci 16.4%(12) and 35.6%(26), respectively.Conclusions The detection rate of pathogens in BALF samples from AIDS patients complicated with pulmonary infection is the highest , followed by sputum and blood samples.

13.
Article in Chinese | WPRIM | ID: wpr-754429

ABSTRACT

Objective: To investigate the application of single-molecule PCR (SM-PCR) in the detection of plasma ctDNA for the treat-ment of patients with advanced lung adenocarcinoma. Methods: In total, 30 patients diagnosed with advanced lung adenocarcinoma were enrolled between June 2017 and May 2018. ctDNA fragments of the target genes (EGFR, KRAS, BRAF, ALK, HER2, and TP53) from the blood samples were enriched by SM-PCR, and DNA libraries were prepared. Finally, a high-throughput sequencing was performed. The EGFR detection of tumor tissue samples was performed using real-time fluorescence PCR based on the amplification refractory mutation system (ARMS) and consistency in the results of EGFR mutation detection in the plasma and tissue was compared. Results:The results of both the methods were consistent (Kappa=0.867, P<0.001). The McNemar's test also indicated that the results are not statistically different (P=0.500). Conclusions: SM-PCR can be used for the detection of plasma EGFR mutations. The target detection sites are more comprehensive and multiple mutations can be detected at the same time. Results of the analysis are more precise and can be absolutely quantified.

14.
Chinese Journal of Pathology ; (12): 856-860, 2019.
Article in Chinese | WPRIM | ID: wpr-801209

ABSTRACT

Objective@#To investigate the expression of LC3B, p-AMPKα and p27 in cortical tuberous sclerosis complex (TSC).@*Methods@#Nineteen specimens of surgically resected TSC cortical tubers were collected at Xuanwu Hospital, Capital Medical University, from 2014 to 2017. The expression of the three proteins in the lesions and the adjacent relatively normal regions was detected by immunohistochemical staining (EnVision two-step method).@*Results@#LC3B was mainly expressed in the dysmorphic neuron and giant cell in TSC cortical tubers and in the adjacent relatively normal neurons, and the expression was diffuse or perinuclear cytoplasmic. There was no significant difference in the average optical density between abnormal cells and neurons adjacent to the lesions (0.343±0.195 vs. 0.419±0.088, P>0.05). p-AMPKα was localized in the cytoplasm of dysmorphic neurons and giant cell in TSC cortical tubers. The average optical density of abnormal cells in the lesions was significantly higher than that of neurons adjacent to the lesions (0.306±0.123 vs. 0.233±0.654, P<0.05). P27 showed nuclear positivity, mainly expressed in the neurons and glial cells close to TSC cortical tubers, while the positive rate in the abnormal cells in TSC cortical tubers was low (15/19 vs. 7/19, P<0.05).@*Conclusion@#There is no significant decrease in the level of autophagy in dysmorphic neurons and giant cells in TSC cortical tubers, which may be related to the compensatory mechanism of AMPK signaling pathway, but without activation of downstream p27.

15.
Chinese Journal of Dermatology ; (12): 337-340, 2018.
Article in Chinese | WPRIM | ID: wpr-710383

ABSTRACT

Objective To investigate the spatial distribution characteristics and patterns of congenital syphilis in China,and to make suggestions and recommendations for precise prevention of motherto-child transmission of syphilis.Methods The geographic information system software ArcGIS 10.0 was used to conduct exploratory spatial data analysis on the basis of congenital syphilis cases reported from 31 provinces,autonomous regions and municipalities directly under the central government in 2010 and 2015.Results In 2015,a total of 7 251 congenital syphilis cases were reported,which decreased by 40.00% compared with the case number in 2010 (12 084 cases).The global spatial autocorrelation analysis revealed a positive spatial autocorrelation in the number of reported cases at the county level in China between 2010 and 2015,with Global Moran's I indices of 0.46 and 0.37,respectively.The degree of spatial clustering of congenital syphilis was lower in 2015 than in 2010.The local spatial autocorrelation analysis was further performed to output the hot-spot mappings of congenital syphilis at the county level in 2010 and 2015.In 2010,207 hot-spot counties with a high number of congenital syphilis cases (> 12 cases) were identified,and these counties were mainly distributed in 3 core areas including southwest China (Chongqing,eastern Sichuan and northern Guizhou),eastern China (southern Jiangsu,Zhejiang and northern Fujian),and southern and central China (Guangxi,Guangdong and mid-east Hunan).In 2015,72 hot-spot counties with a high number of congenital syphilis cases (> 12 cases)were identified,and mainly distributed in 5 core areas including northwest China (western Xinjiang),mid-east Qinghai,southwest and central China (Sichuan,Chongqing,Guizhou and Hunan),eastern China (Jiangsu,Anhui),and southern China and eastern China (Guangdong and Fujian).Conclusions In Zhejiang,Shanghai,Guangdong,Guangxi,Sichuan and Chongqing,the number of hot-spot counties with a high reported congenital syphilis cases was significantly lower in 2015 than in 2010,suggesting that the prevention of mother-to-child transmission of syphilis had achieved a primary success.At present,hot-spots counties with a high number of congenital syphilis cases are mainly distributed in western Xinjiang,mid-east Qinghai,Sichuan,Chongqing,Guizhou and so on,Therefore,the prevention of mother-to-child transmission of syphilis should be strengthened in these areas.

16.
Chinese Journal of Dermatology ; (12): 332-336, 2018.
Article in Chinese | WPRIM | ID: wpr-710382

ABSTRACT

Objective To investigate epidemiological features of genital herpes in China from 2008 to 2017,and to provide scientific evidences for developing control strategies.Methods A descriptive epidemiological study was conducted to analyze personal,spatial and temporal distribution of genital herpes cases reported from 105 sexually transmitted disease (STD) surveillance sites between 2008 and 2017.Results The reported incidence of genital herpes decreased from 8.30 per 100 000 in 2008 to 6.14 per 100 000 in 2017,with an average annual decline rate of 3.29%.There was marked variation in the reported incidence of genital herpes among different STD surveillance sites,with a range from < 1 per 100 000 to 113.51 per 100 000.The reported incidence was relatively high in Guangdong,Guangxi,Hainan,Zhejiang,Fujian,Chongqing and Hubei,but low in north China and central China.In addition,no cases were reported at a few sites in rural areas.The reported annual incidence was higher in females than in males in 2016,but higher in males than in females in the other years.The ratio of male to female cases decreased from 1.77 ∶ 1 in 2008 to 1.03:1 in 2017.Among all the age groups,the sexually active population aged 20-39 years showed high reported incidence of genital herpes,with the highest reported incidence observed in the group aged 25-34 years (range,17.84/100 000-26.89/100 000).Among all kinds of medical institutions,general hospitals had the largest number of reported cases of genital herpes (range,58.13%-76.52%),followed by specialized dermatovenereology hospitals,gynecology hospitals and maternal and child health (MCH) hospitals.Conclusions Although the overall reported incidence of genital herpes shows a downward trend,the reported incidence is high in Guangdong,Guangxi,Hainan,Zhejiang,Fujian,Chongqing and Hubei.Therefore,different prevention strategies are needed to be developed according to the epidemiological features.

17.
China Pharmacy ; (12): 1281-1287, 2018.
Article in Chinese | WPRIM | ID: wpr-704784

ABSTRACT

OBJECTIVE:To evaluate the efficacy and safety of Yupingfeng powder combined with second- generation antihistamines versus second-generation antihistamines for chronic urticaria(CU)systematically,and to provide evidence-based reference for clinical treatment for CU. METHODS:Retrieved from PubMed,Embase,The Cochrane Library,CJFD,VIP and CBM,RCT about therapeutic efficacy(total response rate,cure rate,recurrence rate)and safety(the incidence of ADR)of Yupingfeng combined with second-generation antihistamines(trial group)versus second-generation antihistamines(control group) in the treatment of CU were collected. The data extraction was performed for included clinical studies,and Meta-analysis was performed by using Rev Man 5.3 statistical software after quality evaluation with Cochrane Handbook 5.1.0 evaluation criteria. RESULTS:A total of 34 RCTs were enrolled,involved 3 405 patients in total. Results of Meta-analysis showed that the total response rate [OR=4.02,95%CI(3.03,5.34),P<0.001],cure rate [OR=2.25,95%CI(1.95,2.60),P<0.001] and recurrence rate [OR=0.33,95%CI(0.26,0.42),P<0.001] of trial group were significantly better than those of control group,with statistical significance. There was no statistical significance in the incidence of ADR between 2 groups [OR=0.98,95%CI(0.71,1.37),P=0.92]. CONCLUSIONS:For CU therapy,Yupingfeng powder combined with second-generation antihistamines is better than second-generation antihistamines alone in improving total response rate and cure rate,reducing recurrence rate,both have similar safety.

18.
Article in Chinese | WPRIM | ID: wpr-703283

ABSTRACT

The proliferation of tumor cells is regulated by a complex array of signaling pathways, among these signaling pathways,the programmed cell death. Autophagy and apoptosis are two types of programmed death. There are significant differences in their morphological and functional features, but they also have many links. Both apoptosis and autophagy are involved in activation,expression and regulation of a series of genes. By reviewing the research progress in recent years, this article will discuss the cellular regulation and molecular mechanisms of their related genes. Through summarizing the relationship between autophagy and apoptosis,it aims to get a better understanding of the mechanisms of autophagy and apoptosis in tumor progression,and looking at the perspectives for studies on the autophagy and apoptosis in tumor treatment.

19.
Article in Chinese | WPRIM | ID: wpr-703222

ABSTRACT

Objective To explore the biological indicators of diagnosis and treatment of irritable bowel syndrome (IBS), and to explore the mechanism of action of a Chinese medicine Wuji Pill (WJW) on irritable bowel syndrome (IBS). Methods (1) Postinflammatory irritable bowel syndrome (PI-IBS) rat model was established by acetic acid plus restraint stress method . (2) The colonic motor ability of rats was evaluated by colon motility index (MI), the number of fecal particles discharged within 2 h, and the time of glass pellet discharge. (3) The formation of PI-IBS model rats and the therapeutic effect of WJW were observed. (4) The levels of calcitonin gene-related peptide (CGRP), motilin (MTL), neuropeptide Y (NPY), substance P (SP), somatostatin (SS), vasoactive intestinal peptide (VIP), and cholecystokinin (CCK) in the brain and colon tissues of PI-IBS rats were measured by ELISA. Results (1) The rat PI-IBS model was successfully established. Compared with the normal group, the body weight of the model rats was decreased, the food intake decreased, the amount of feces increased, loose stools and amorphous soft stools appeared, voluntary movements decreased, colon motility index ( MI) significantly increased ( P < 0. 05 ), the number of fecal particles discharged significantly increased ( P< 0. 05), and the glass pellet discharge time was significantly shortened ( P < 0. 05). (2) WJW treatment for 7 days significantly improved a variety of symptoms. Compared with the normal control, the levels of CGRP, SS and VIP in the brain tissue of PI-IBS rats were significantly increased (P< 0. 05), and the NPY concentration was significantly decreased ( P < 0. 05). However, the treatment with WJW significantly reduced CGRP, SS and VIP levels (P< 0. 05), and significantly increased the NPY concentration level (P < 0. 05). (3) Compared with the normal control group, the levels of CCK, NPY, MTL, SS and VIP in colonic tissues of PI-IBS rats were significantly decreased (P< 0. 05), while WJW significantly increased the CCK and VIP levels. Conclusions WJW can be used to treat IBS by regulating the levels of various brain-gut peptides in the brain and colon tissues of IBS rats. These anomalous and adjustable brain-gut peptides may become a potential biomarker for the diagnosis and treatment of IBS.

20.
Chinese Pediatric Emergency Medicine ; (12): 898-902,906, 2018.
Article in Chinese | WPRIM | ID: wpr-733495

ABSTRACT

Stroke is among the top 10 causes of death and a leading cause of childhood disability. Pediatric arterial ischemic stroke (AIS) differs from adult AIS in several ways. There are many risk factors among which non-atherosclerotic arterial disease and heart disease are the most frequently identified risk factors of AIS in children. Clinical manifestations do vary with age. The clinical manifestations are extremely atypical in neonates and infants. The treatment and prognosis are obviously different from that of adults. This review will discuss important developments in childhood arterial ischemic stroke, focus on improved understanding of the risk factors,clinical manifestations,consequences and targets for intervention.

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