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1.
Article in English | WPRIM | ID: wpr-913816

ABSTRACT

Purpose@#NUF2 has been implicated in multiple cancers recently, suggesting NUF2 may play a role in the common tumorigenesis process. In this study, we aim to perform comprehensive meta-analysis of NUF2 expression in the cancer types included in the Cancer Genome Atlas (TCGA). @*Materials and Methods@#RNA-sequencing data in 31 cancer types in the TCGA data and 11 independent datasets were used to examine NUF2 expression. Silencing NUF2 using targeting shRNAs in hepatocellular carcinoma (HCC) cell lines was used to evaluate NUF2’s role in HCC in vitro and in vivo. @*Results@#NUF2 up-regulation is significantly observed in 23 out of the 31 cancer types in the TCGA datasets and validated in 13 major cancer types using 11 independent datasets. NUF2 overexpression was clinically important as high NUF2 was significantly associated with tumor stages in eight different cancers. High NUF2 was also associated with significantly poorer patient overall survival and disease-free survival in eight and six cancers, respectively. We proceeded to validate NUF2 overexpression and its negative association with overall survival at the protein level in an independent cohort of 40 HCC patients. Compared to the non-targeting controls, NUF2 knockdown cells showed significantly reduced ability to grow, migrate into a scratch wound and invade the 8 μm porous membrane in vitro. Moreover, NUF2 knockdown cells also formed significantly smaller tumors than control cells in mouse xenograft assays in vivo. @*Conclusion@#NUF2 up-regulation is a common feature of many cancers. The prognostic potential and functional impact of NUF2 up-regulation warrant further studies.

2.
Chinese Journal of Trauma ; (12): 1068-1073, 2021.
Article in Chinese | WPRIM | ID: wpr-909978

ABSTRACT

Objective:To investigate the risk factors of muscle necrosis in patients with acute compartment syndrome(ACS).Methods:A retrospective case-control study was conducted for clinical data of 111 ACS patients admitted to West China Hospital, Sichuan University from January 2010 to December 2020, including 84 males and 27 females; age 18-76 years [45(36, 55)years]. Muscle necrosis was presented in 35 patients(necrotic muscle group), but was not seen in 76 patients(non-necrotic muscle group). The univariate analysis was performed for the two groups in the demographic data(sex, age, ethnicity, body mass index, smoking history, chronic comorbidities), injury patterns [ mechianism of injury(low energy injury, high energy injury, crush injury, other injury), time from injury to treatment, first visit or not, combination with bone fracture or not, open injury or not, presence of tension blisters or not], medical treatment(number of debridements, fasciotomy or not)and laboratory indicators [hemoglobin(Hb), platelet count(PLT), white blood cell count(WBC), prothrombin time(PT), international normalized ratio(INR), partially activated prothrombin time(APTT), fibrinogen(FIB), D-Dimer(D-D), alanine aminotransferase(ALT), aspartate aminotransferase(AST), albumin(ALB), intravenous blood glucose(GLU), creatine kinase(CK), peak value of CK during hospitalization(natural logarithmic conversion, lnCK), serum sodium(NA), serum potassium(K), serum calcium(CA)]. Further multivariate logistic regression was performed to analyze the independent risk factors of muscle necrosis in ACS patients.Results:The univariate analysis showed that there were statistically significant differences between the two groups in the mechanism of injury, first visit or not, combination with bone fracture or not, number of debridements, Hb, PT, INR, D-D, AST, ALB, GLU, CK and lnCK( P<0.05), while not in the basic data, time from injury to treatment, open injury or not, presence of tension blisters or not, fasciotomy or not, PLT, WBC, APTT, FIB, ALT, NA, K and CA( P>0.05). The multivariate logistic regression analysis showed that high energy injury( OR=5.143, 95% CI 1.216-21.758, P<0.05), crush injury( OR=22.313, 95% CI 2.625-189.635, P<0.05), other mechanism of injury( OR=9.019, 95% CI 1.036-78.554, P<0.05), first visit or not( OR=0.071, 95% CI 0.006-0.819, P<0.05), Hb( OR=0.979, 95% CI 0.961-0.998, P<0.05), GLU( OR=1.218, 95% CI 1.020-1.455, P<0.05)and lnCK( OR=1.805, 95% CI 1.235-2.639, P<0.05)were significantly related with muscle necrosis. Conclusion:The mechanism of injury, first visit or not, Hb, GLU and lnCK are the independent risk factors of muscle necrosis in patients with ACS.

3.
Chinese Journal of Trauma ; (12): 726-732, 2021.
Article in Chinese | WPRIM | ID: wpr-909929

ABSTRACT

Objective:To investigate the risk factors of multi-drug resistant organism(MDRO)infection in orthopedic trauma patients.Methods:A retrospective case series study was made on clinical data of 95 orthopedic patients with in-hospital wound infection admitted to West China Hospital of Sichuan University from January 2020 to December 2020,including 71 males and 24 females at age of 14-70 years[(42.6 ± 13.8)years]. MDRO infection occurred in 38 patients(DRO group),while not in 57 patients(non-MDRO group). Etiological characteristics of wound infections were observed. Univariate analysis was used to compare variables between the two groups,including baseline data(gender,age,length of hospital stay,chronic comorbidity)and injury as well as hospitalization information(transfer from another hospital,emergency admission,time from injury to first treatment,open injury,combination with fracture,wound contamination,depth of wound,ICU stay,number of operations,cumulative operation time,type of incision,length of hospital stay before diagnosis of infection,type of antibiotics used,usage of hormones/immunosuppressive agents,level of hemoglobin/serum albumin/blood glucose on admission). Binary Logistic regression was used to analyze independent risk factors for MDRO infection.Results:A total of 119 pathogenic bacteria were detected on the wound,including 21 gram-positive cocci(18%),91 gram-negative bacilli(76%)and 7 others(6%). Top five strains were Acinetobacter baumannii,Enterobacter cloacae,Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa. Univariate analysis showed the two groups had significant differences in terms of length of hospital stay,wound contamination,ICU stay,length of hospital stay before diagnosis of infection,type of antibiotics used and levels of hemoglobin and serum albumin on admission( P < 0.05),but not in gender,age,hypertension,diabetes,transfer from another hospital and emergency admission( P > 0.05). Multivariate Logistic regression analysis showed MDRD infection was obviously correlated with the long hospital stay( OR = 1.033, 95%CI 1.005-1.061, P < 0.05),time from injury to first treatment over 6 hours( OR = 4.282, 95%CI 1.174-15.616, P < 0.05),types of antibiotics used ≥ 3( OR = 7.486, 95%CI 2.451-22.863, P < 0.05)and low level of hemoglobin on admission( OR = 0.973, 95%CI 0.962-0.985, P < 0.05). Conclusions:For orthopedic trauma patients,MDRO infection is independently associated with long hospital stay,time from injury to first treatment,type of antibiotics use and level of hemoglobin on admission,suggesting that decreased length of hospitalization,shortened time of first wound treatment,individualized and rational usage of antibiotics and timely correction of anemia are important measures to prevent MDRO infection.

4.
Article in Chinese | WPRIM | ID: wpr-907121

ABSTRACT

ObjectiveTo analyze the effects of respiratory control measures before and after COVID-19 epidemic on influenza virus. MethodsThe percentage of influenza-like cases, the positive rate of influenza virus and the change of influenza outbreaks before and after the COVID-19 pandemic were compared and analyzed by selecting the data of influenza surveillance sentinel-points in Shanghai. ResultsThe percentage of influenza-like illness after the outbreak of COVID-19 in 2020 was significantly higher than that during the same period between 2017 and 2019. The positive rate of influenza virus detection in 2020 was significantly lower than the average rate of influenza virus detection from 2017 to 2019 with significant statistical difference (χ 2=2 359.07, P<0.001). The number of outbreaks in 2020 was significantly lower than that from 2017 to 2019. ConclusionDuring the respiratory season, personal protection and reduction of human aggregation can effectively reduce the infection of influenza and the incidence of influenza in the population.

5.
Article in Chinese | WPRIM | ID: wpr-907098

ABSTRACT

ObjectiveTo analyze the effects of respiratory control measures before and after COVID-19 epidemic on influenza virus. MethodsThe percentage of influenza-like cases, the positive rate of influenza virus and the change of influenza outbreaks before and after the COVID-19 pandemic were compared and analyzed by selecting the data of influenza surveillance sentinel-points in Shanghai. ResultsThe percentage of influenza-like illness after the outbreak of COVID-19 in 2020 was significantly higher than that during the same period between 2017 and 2019. The positive rate of influenza virus detection in 2020 was significantly lower than the average rate of influenza virus detection from 2017 to 2019 with significant statistical difference (χ 2=2 359.07, P<0.001). The number of outbreaks in 2020 was significantly lower than that from 2017 to 2019. ConclusionDuring the respiratory season, personal protection and reduction of human aggregation can effectively reduce the infection of influenza and the incidence of influenza in the population.

6.
Acta Pharmaceutica Sinica ; (12): 3547-3554, 2021.
Article in Chinese | WPRIM | ID: wpr-906827

ABSTRACT

According to the commonly used tablet compressibility, compactability and tabletability equation, the influence of pressure range on the fitting results and parameters of different compression equations was studied, and the optimal pressure range of different equations was determined. Plastic material microcrystalline cellulose (MCC) PH102, brittle material spray dried lactose and Chinese medicine Sanqi were used as experimental objects, the compression curves of tablets were obtained by the combination of dies with different diameters. For Heckel equation, the shape of Heckel section of different materials is not uniform, and the specified linear fitting range cannot be obtained, therefore, different distances between fitting pressure starting point and starting point were set to observe the influence of pressure range on R2 of Heckel equation. The Kawakita equation, Gurnham equation, Ryshkewitch-Duckworth (R-D) equation and Power equation are fitted in three different pressure ranges of 15-200, 15-300 and 15-400 MPa, respectively. In order to find the best linear region of Heckel equation, the 3D scatter diagram of "starting point of pressure, pressure range and R2" is drawn. The best linear pressure ranges of Heckel curves of MCC, lactose and Sanqi were 20-170, 20-220 and 10-90 MPa, respectively. It is proved that the 3D scatter diagram is an effective method to find the linear range of Heckel equation. The change of pressure range has little influence on the curve fitting effect and compression parameters of Kawakita equation, Gurnham equation and Ryshkewitch-Duckworth equation. The low pressure range of 15-200 MPa can meet the fitting requirements of Kawakita equation, Gurnham equation, R-D equation and Power equation for different materials. Therefore, only by optimizing the pressure range, can the good fitting effect be ensured and the obtained compression parameters be more reliable and interpretable.

7.
Article in Chinese | WPRIM | ID: wpr-906349

ABSTRACT

Traditional Chinese medicine (TCM) and western medicine have their respective advantages and limitations in the diagnosis and treatment of common otorhinolaryngology head and neck diseases. Although the integrated TCM and western medicine exhibits definite curative effects, there is no consensus on the otorhinolaryngology head and neck diseases responding specifically to TCM or integrated TCM and western medicine, as well as the diagnosis and treatment schemes. The China Association of Chinese Medicine (CACM) thus organized the otorhinolaryngology head and neck specialists of both TCM and western medicine to discuss the etiology, pathogenesis, and clinical diagnosis and treatment methods of common otorhinolaryngology head and neck diseases with the results of multiple clinical trials taken into account. The acute pharyngitis, chronic pharyngolaryngitis, paraesthesia pharyngis, hysterical aphasia, allergic rhinitis, subjective tinnitus, and otogenic vertigo were confirmed to respond specifically to TCM or integrated TCM and western medicine. Then a mutually agreed diagnosis and treatment scheme and recommendation with integrated TCM and western medicine was formulated as a reference for clinical practice, thus benefiting more patients.

8.
Article in Chinese | WPRIM | ID: wpr-906027

ABSTRACT

Objective:To investigate the influence of particle size on density of binary powder mixture of traditional Chinese medicine (TCM), and to provide reference for formulation design of TCM preparations. Method:Three groups of binary powder mixtures with different particle size ratio (<italic>α</italic>) were constructed, namely Oroxyli Semen-microcrystalline cellulose PH102 (MCC PH102) (<italic>α</italic>=0.071 7), Stellariae Radix-MCC PH200 (<italic>α</italic>=0.158 7) and Angelicae Sinensis Radix-MCC KG802 (<italic>α</italic>=0.840 6). Binary powder mixtures with nine mass ratios (90∶10, 80∶20, 70∶30, 60∶40, 50∶50, 40∶60, 30∶70, 20∶80 and 10∶90) were prepared for each group, and 27 binary powder mixtures containing TCM were obtained. The particle size distribution, density and other parameters of six single materials and 27 binary powder mixtures were characterized. Based on the packing theory and multivariate analysis, the effects of particle size related parameters on the filling structure and density of the binary powder mixtures were elucidated. Result:The <italic>α</italic> of Angelicae Sinensis Radix-MCC KG802 binary mixture system was larger than the replacement rate (<italic>α</italic><sub>r</sub>=0.741 0), and its density had a good linear relationship with the mass ratio, which conformed to the replacement mechanism. The <italic>α</italic> of Oroxyli Semen-MCC PH102 binary mixture system was smaller than the critical ratio (<italic>α</italic><sub>c</sub>=0.154 0), and its density was nonlinear with the mass ratio of components, which conformed to the filling mechanism. The <italic>α</italic> of Stellariae Radix-MCC PH200 binary mixture system was between <italic>α</italic><sub>c</sub> and <italic>α</italic><sub>r</sub>, its density was affected by both of replacement mechanism and filling mechanism. Based on the partial least squares (PLS) model, the variable importance in the projection (VIP) analysis further proved that the mixing mass ratio (VIP value=1.62), <italic>α</italic> (VIP value=1.13) and <italic>D</italic><sub>10</sub> (the corresponding particle size when the particle size distribution accumulated to 10%, VIP value=1.06) were the key factors affecting the density of binary powder mixtures of TCM. Conclusion:In the binary powder mixtures of TCM, the linearity relationship between density and mass ratio is largely depended on particle size difference of components.

10.
Chinese Journal of Dermatology ; (12): 421-427, 2021.
Article in Chinese | WPRIM | ID: wpr-885231

ABSTRACT

Objective:To investigate the correlation between variations in mitochondrial DNA (mtDNA) control region (D-loop) and keloids.Methods:A total of 216 patients with keloids were collected from Department of Dermatology, the First Affiliated Hospital of Kunming Medical University from 2016 to 2019. Total DNA was extracted from peripheral blood samples of all the patients, as well as keloid tissues and perilesional normal skin tissues of 25 patients with keloids. Peripheral blood samples were collected from 299 health checkup examinees without keloids in Health Examination Center, the Affiliated Hospital of Yunnan University, who served as controls. PCR amplification and Sanger sequencing were performed on the mtDNA D-loop region, and mutation sites in each sample were analyzed by comparisons with the revised Cambridge Reference Sequence (rCRS) . Haplogroups were assigned in the 2 groups by using Phylotree-mtDNA tree Build 17. Mutations in the mtDNA D-loop region were compared among keloid tissues, perilesional normal skin tissues and peripheral blood samples. A median-joining network was constructed via network 5.0 software. Binary logistic regression analysis was performed to investigate the correlation between haplogroup frequencies and the occurrence of keloids, and chi-square, t and t′ tests were used to analyze clinical data. Results:Among the 216 patients with keloids, variations in mtDNA D-loop region were classified into 10 haplogroups, including A, B, D, R9, G, M*, M7, M8, M9 and N9, with the haplogroups R9 and M9 showing the highest (21.3%, 46/216) and lowest (0.9%, 2/216) frequencies respectively. The frequencies of haplogroups M7 ( P=0.040, OR=0.248, 95% CI: 0.066 - 0.937) and N9 ( P=0.048, OR=0.191, 95% CI: 0.037-0.986) were significantly lower in the patients with keloids than in the controls. The median-joining network plot showed that the distribution pattern of the haplogroup M7 differed between the patients with keloids and controls. Significantly less number of lesional sites and younger age of onset were observed in the patients with haplogroup M7 compared with those with non-M7 haplogroups ( P=0.000 1, 0.045, respectively) . Conclusion:The haplogroup M7 is correlated with the occurrence of keloids, and may be a potential protective factor for keloid formation.

11.
Article in Chinese | WPRIM | ID: wpr-884809

ABSTRACT

Objective:To summarize the characteristics of 18F-fluorodeoxyglucose (FDG) PET/CT imaging in adult patients with infectious mononucleosis (IM). Methods:18F-FDG PET/CT imaging data and clinical data of 5 IM patients (all males, age 18-77 years) detected in the Department of Nuclear Medicine of the East Hospital Affiliated to Tongji University from July 2015 to July 2019 were analyzed retrospectively. Distribution of lymphatic tissues in the lesions (lymph nodes, tonsils, bone marrow, liver, etc) and 18F-FDG radioactive uptake in the lesions were analyzed semiquantitatively. Results:In 5 patients with IM, the volume of superficial lymph nodes (mainly distributed in the neck, axilla, and groin lymph nodes) and deep lymph nodes (mainly distributed in the mediastinum, hilum of lung, abdominal cavity and posterior abdominal cavity) increased to varying degrees, as well as the FDG uptake. Maximum standardized uptake value (SUV max) of superficial lymph nodes were 1.2-7.3, and those of deep lymph nodes were 3.5-9.7. All patients showed diffuse uptake of FDG in bone marrow, with SUV max of 3.0-7.9. All patients had pharyngeal tonsillar enlargement and FDG uptake increasing, with SUV max of 1.7-13.4. Compared with patients with lymphoma, IM patients had more organ involvement and relatively lower radioactive uptake. Conclusion:18F-FDG PET/CT imaging features of IM can help the diagnosis of IM.

12.
Article in Chinese | WPRIM | ID: wpr-882022

ABSTRACT

This article summarized the laws and regulations on the prevention and control of infectious diseases, classification of infectious diseases, and measures for the prevention and control of infectious diseases in Japan. Ⅰt focused on the containment of spread of infectious diseases, classification and categorization in the treatment and quarantine. Ⅰn addition, the response to the novel coronavirus pneumonia in the early stage of transmission in Japan was introduced. Moreover, we propose specific suggestions to improve the system of laws and regulations on the prevention and control of infectious diseases in China, improve the classification of infectious diseases, clarify the rights and obligations of governments at all levels, establish medical facilities with"classification and combination of normal and emergency situations"for the treatment of infectious diseases, practice emergency simulation exercises regularly, and improve risk communication.

13.
Article in English | WPRIM | ID: wpr-878357

ABSTRACT

Objective@#The aim of the present study was to evaluate the performance of the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA using one dried blood spot (DBS) as an alternative sample to plasma.@*Method@#A total of 571 paired DBS/plasma samples were collected from men who have sex with men (MSM) and injection drug users (IDUs), and serological and molecular assays were performed. Using plasma results as the reference standard, the performance of DBS tests for HIV-1 RNA, HIV-1 DNA, and HCV RNA was evaluated. Pearson's correlation coefficients and Bland-Altman analysis were performed to assess the correlation and concordance between DBS and plasma.@*Results@#Among paired plasma/DBS samples with detectable HIV-1 RNA and HCV RNA, five samples (5/32) were not detectable in DBS, while measurable HIV-1 RNA levels were present in plasma (1.44 to 3.99 log @*Conclusion@#The performance of the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA using one DBS was acceptable. DBS, as an alternative sample to plasma, may be a viable option for the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA in resource-limited settings or for individuals living in areas that are difficult to access.


Subject(s)
DNA, Viral/analysis , Diagnostic Tests, Routine/methods , Dried Blood Spot Testing/methods , HIV Infections/diagnosis , HIV-1/isolation & purification , Hepacivirus/isolation & purification , Hepatitis C/diagnosis , RNA, Viral/analysis , Sensitivity and Specificity , Specimen Handling/methods , Syphilis/diagnosis , Treponema pallidum/isolation & purification
14.
China Pharmacy ; (12): 702-708, 2021.
Article in Chinese | WPRIM | ID: wpr-875651

ABSTRACT

OBJECTIVE:To study the improvement effect and mechanism of MEBO on lipopolysaccharide (LPS)-induced injury of rat skin fibroblasts. METHODS :Skin fibroblasts of rats were divided into control group ,LPS group (5 μg/mL), Kangfuxin solution group (positive control ,5 μg/mL LPS+1.25% Kangfuxin solution )and MEBO group (5 μg/mL LPS+0.6 mg/mL MEBO),with 6 wells in each group. Inflammatory injury cell model was induced by LPS (except for control group ). After a certain period of cultivation ,the cell survival rate and cell migration rate were detected in each group. The contents of TNF-α and IL-6 in cell supernatant was detected. The localization and fluorescence intensity of IL- 6 protein were detected. The protein expression of PTEN ,p-p65,TNF-α,IL-6,PI3K and Akt in the fibroblasts were also determined. RESULTS :Compared with control group ,survival rate of the fibroblasts was increased significantly in LPS group ,while cell migration was decreased significantly;the contents of TNF-α and IL-6 in cell supernatant as well as relative protein expression of PTEN ,p-p65,TNF-α, IL-6 and PI 3K were increased significantly (P<0.05 or P<0.01);IL-6 protein mainly expressed in the cytoplasm ,and the fluorescence intensity was enhanced. Compared with LPS group ,survival rate of the fibroblasts was decreased significantly in Kangfuxin solution group and MEBO group ,while migration rate was increased significantly ;the contents of TNF-α and IL-6, relative protein expression of PTEN ,p-p65,TNF-α,IL-6(except for Kangfuxin solution group ),PI3K and Akt (except for Kangfuxin solution group ) were decreased significantly (P<0.05 or P<0.01),while fluorescence intensity of IL- 6 protein decreased;relative protein expression of TNF-α,IL-6,PI3K and Akt in MEBO group were significantly lower than Kangfuxin solution group (P<0.05 or P<0.01). CONCLUSIONS :MEBO can inhibit the proliferation of LPS-induced skin fibroblasts , reduce the level of inflammatory factors and the intensity of inflammatory reaction , which may be related to the jiang- down-regulation of PTEN/NF-κB,PI3K/Akt signaling pathway.

15.
Article in English | WPRIM | ID: wpr-895327

ABSTRACT

Objectives@#To compare the effect of eldecalcitol and alfacalcidol on skeletal microstructure by highresolution peripheral QCT (HR-pQCT). @*Methods@#This was a substudy of a randomized, double-blind, active comparator trial. Five female osteoporotic patients with 1-year 0.75 mg/day eldecalcitol and 5 with 1-year 1.0 mg/day alfacalcidol completed HR-pQCT scans before and after treatment were enrolled. @*Results@#Total vBMD [1.67 ± 1.06% (mean ± SD), P ¼ 0.043 versus baseline] and trabecular vBMD (2.91 ± 1.72%, P ¼ 0.043) at the radius increased in eldecalcitol group, while total, trabecular, and cortical vBMD tended to decrease in alfacalcidol group, with a significant reduction in cortical vBMD at the tibia (0.88 ± 0.62%, P ¼ 0.043). Cortical area (1.82 ± 1.92%, P ¼ 0.043) at the radius and thickness (0.87 ± 1.12%, P ¼ 0.043) at the tibia increased in eldecalcitol group, while these parameters decreased with alfacalcidol at the tibia (1.77 ± 1.72%, P ¼ 0.043 for cortical area; 1.40 ± 2.14%, P ¼ 0.042 for cortical thickness). Trabecular thickness at the radius (1.97 ± 1.93%, P ¼ 0.042) and number at the tibia (3.09 ± 3.04%, P ¼ 0.043) increased by eldecalcitol but did not increase by alfacalcidol. Trabecular separation decreased by eldecalcitol (2.22 ± 2.43%, P ¼ 0.043) but tended to increase by alfacalcidol at the tibia. @*Conclusions@#Eldecalcitol has the greater potential to improve cortical and trabecular microstructure at the peripheral bone than alfacalcidol which needs further more studies.

16.
Article in English | WPRIM | ID: wpr-922104

ABSTRACT

OBJECTIVE@#To elucidate the underlying mechanism of Panax notoginseng saponin (PNS) on gastric epithelial cell injury and barrier dysfunction induced by dual antiplatelet (DA).@*METHODS@#Human gastric mucosal epithelial cell (GES-1) was cultured and divided into 4 groups: a control, a DA, a PNS+DA and a LY294002+PNS+DA group. GES-1 apoptosis was detected by flow cytometry, cell permeability were detected using Transwell, level of prostaglandins E2 (PGE2), 6-keto-prostaglandin F1α (6-keto-PGF1α) and vascular endothelial growth factor (VEGF) in supernatant were measured by enzyme linked immunosorbent assay (ELISA), expression of phosphatidylinositide 3-kinase (PI3K), phosphorylated-PI3K (p-PI3K), Akt, phosphorylated-Akt (p-Akt), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), glycogen synthase kinase-3β (GSK-3β) and Ras homolog gene family member A (RhoA) were measured by Western-blot.@*RESULTS@#DA induced apoptosis and hyper-permeability in GES-1, reduced supernatant level of PGE2, 6-keto-PGF1α and VEGF (P<0.05). Addition of PNS reduced the apoptosis of GES-1 caused by DA, restored the concentration of PGE2, 6-keto-PGF1α and VEGF (P<0.05). In addition, PNS attenuated the alteration of COX-1 and COX-2 expression induced by DA, up-regulated p-PI3K/p-Akt, down-regulated RhoA and GSK-3β. LY294002 mitigated the effects of PNS on cell apoptosis, cell permeability, VEGF concentration, and expression of RhoA and GSK-3β significantly.@*CONCLUSIONS@#PNS attenuates the suppression on COX/PG pathway from DA, alleviates DA-induced GES-1 apoptosis and barrier dysfunction through PI3K/Akt/ VEGF-GSK-3β-RhoA network pathway.


Subject(s)
Cyclooxygenase 1 , Epithelial Cells/metabolism , Glycogen Synthase Kinase 3 beta , Humans , Panax notoginseng , Phosphatidylinositol 3-Kinases/metabolism , Platelet Aggregation Inhibitors , Proto-Oncogene Proteins c-akt/metabolism , Saponins/pharmacology , Vascular Endothelial Growth Factor A , rhoA GTP-Binding Protein
17.
Article in Chinese | WPRIM | ID: wpr-921750

ABSTRACT

The calibration of chromone reference extract(CRE) was conducted and a quality control method of Saposhnikoviae Radix(SR) was established based on CRE. Meanwhile, the quality control system of SR was improved and the feasibility of using reference extract as a substitute for single reference substance in quality control of Chinese medicine was discussed. In this study, the content of the prepared CRE was calibrated with prim-O-glucosylcimifugin, cimifugin, 4'-O-β-D-glucosyl-5-O-methylvisamminol, and secO-glucosylhamaudol as indicators. Subsequently, an HPLC analytical method was developed to determine the content of four chromones in 20 batches of SR samples based on the CRE with known content as the standard substance. T-test was used for the comparison of the determination results of the two methods(single chemical component and CRE as reference substances, respectively), and the P values of prim-O-glucosylcimifugin, cimifugin, 4'-O-β-D-glucosyl-5-O-methylvisamminol, and sec-O-glucosylhamaudol were 0. 16,0. 39, 0. 14, and 0. 42. The results demonstrated that there was no significant difference between the two methods. This study initially verified the feasibility that the CRE could be used as a substitute for single reference substance in quality control of SR. In conclusion,this study is expected to provide a scientific basis and a new research model for the application of reference extract in the quality control of Chinese medicine.


Subject(s)
Apiaceae , Calibration , Chromatography, High Pressure Liquid , Chromones , Drugs, Chinese Herbal , Quality Control
18.
Article in Chinese | WPRIM | ID: wpr-921634

ABSTRACT

The high shear wet granulation(HSWG) process of Chinese medicine has a complicated mechanism. There are many influencing factors that contribute to this process. In order to summarize the manufacturability of different kinds of materials in HSWG, this paper constructed a material library composed of 11 materials, including 4 Chinese medicine extracts and 7 pharmaceutical excipients. Each material was described by 22 physical parameters. Several binders were employed, and their density, viscosity and surface tension were characterized. Combining empirical constraints and the principle of randomization, 21 designed experiments and 8 verification experiments were arranged. The partial least squares(PLS) algorithm was used to establish a process model in prediction of the median granule size based on properties of raw materials and binders, and process parameters. The surface tension and density of binders, as well as the maximum pore saturation were identified as key variables. In the latent variable space of the HSWG process model, all materials could be divided into three categories, namely the Chinese medicine extracts, the diluents and the disintegrants. The granulation of Chinese medicine extracts required low viscosity and low amount of binder, and the resulted granule sizes were small. The diluent powders occupied a large physical space, and could be made into granules with different granule sizes by adjusting the properties of binders. The disintegrants tended to be made into large granules under the condition of aqueous binder. The combination use of material database and multivariate modeling method is conducive to innovate the knowledge discovery of the wet granulation process of Chinese medicine, and provides a basis for the formulation and process design based on material attributes.


Subject(s)
Drug Compounding , Excipients , Medicine, Chinese Traditional , Particle Size , Powders , Tablets , Technology, Pharmaceutical
19.
Article in Chinese | WPRIM | ID: wpr-879869

ABSTRACT

A boy attended the hospital at the age of 1 month due to left hand tremor for 1 week. A blood test showed a reduction in serum uric acid and a cranial MRI showed encephalomalacia, atrophy, and cystic changes. The boy had microcephalus, unusual facial features (long face, long forehead, protruded forehead, long philtrum, low nasal bridge, facial swelling, and thick lower lip), hypertonia of lower extremities, and severe global developmental delay. Whole-exome sequencing performed for the boy detected a homozygous mutation, c.217C > T(p.R73W), in the


Subject(s)
Carbon-Carbon Lyases , China , Humans , Infant, Newborn , Male , Metal Metabolism, Inborn Errors , Mutation , Uric Acid
20.
Article in Chinese | WPRIM | ID: wpr-879199

ABSTRACT

Chinese traditional medicine compound is the main form of Chinese medicine clinical application. The elucidation of the effective components of traditional Chinese medicine is one of the key scientific issues to promote the modernization of traditional Chinese medicine. At present, there are many research ideas on the effective components of traditional Chinese medicine compounds. By analyzing the current status and existing problems of existing research ideas, the author proposes a "double reduction network pharmacology"(2 R network pharmacology) research method based on "prediction of dominant components-potential target selection". Chemical components with good properties were selected by ADMET property prediction technology, and compared with the blood components and target organ components to determine the dominant components with potential therapeutic effect, that is "reducing constituents"; the potential core regulatory pathway of traditional Chinese medicine compound was enriched by RNA-Seq technology combined with network database, and then the target of traditional Chinese medicine compound was mined based on the signal pathway, that is "reducing targets". To improve the efficiency and accuracy of effective component screening, the network relationship of "component target" was established by the related technology of network pharmacology. The purpose of this study is to provide practical research ideas and methods for clarifying the effective components of traditional Chinese medicine, revealing the law of compatibility of traditional Chinese medicine and clarifying the target of drug action.


Subject(s)
Databases, Factual , Drugs, Chinese Herbal/pharmacology , Medicine, Chinese Traditional , Molecular Docking Simulation , Research Design
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