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1.
Article in Chinese | WPRIM | ID: wpr-906129

ABSTRACT

Sanrentang, originally contained in the Regulations on Febrile Diseases written by WU Ju-tong in the Qing dynasty, was composed of eight traditional Chinese herbs to treat damp-warm diseases. It is a treatment method of gradually clearing away damp heat of tri-jiao, with characteristics of separating dispersion and mobilizing discharge. "Separating dispersion" means dispersion in separated ways, with different ways to eliminate dampness to export the dampness, heat, evil and turbid out of the body. "Mobilizing discharge" means discharge to stretch and unblock the Qi, to get rid of dampness and evil. It can be seen, Sanrentang, as a desiccating formula, taking "separating dispersion and mobilizing discharge" as the cubic basis, has a significant effect on both internal and external dampness associated with pathogenic heat syndrome, and its clinical application is quite extensive. After consulting the data of the past 10 years, the authors gave a brief overview on the syndrome theory, clinical application and pharmacological effects of Sanrentang, and elaborated the therapeutic effect and pharmacological effect of Sanrentang in the clinical application of upper, middle and lower Tri-jiao respectively, providing theoretical reference for effective development and utilization of Sanrentang. Coronavirus disease-2019(COVID-19) swept the world in early 2020 and it is a great challenge for the medical community to seek for effective prevention and treatment methods. For COVID-19, although the cause of the disease belongs to the Qi of "pestilence", many doctors have different opinions on the pathogenic characteristics. However, they all agree with the clinical characteristics of "dampness". COVID-19, which has the attribute of "dampness evil", is so sticky that it can't be cured at once. In addition to the main symptoms such as fever and cough, it is also characterized by the middle-jiao dampness symptoms such as poor appetite, fullness of abdomen, loose stool and diarrhea. It is worthy to further explore the application prospect of Sanrentang in COVID-19 prevention and treatment.

2.
Article in Chinese | WPRIM | ID: wpr-905963

ABSTRACT

Objective:To investigate the action mechanism of Yinchenhao Tang against type 2 diabetes mellitus (T2DM) complicated with non-alcoholic fatty liver disease (NAFLD) in MKR mice. Method:Forty eight-week-old MKR mice were fed a high-fat diet for eight weeks and then divided into the model group,original Yinchenhao Tang (17.16 g·kg<sup>-1</sup>) group,Yinchenhao Tang group at a specified dose (4.68 g·kg<sup>-1</sup>) in teaching materials,and positive drug [metformin + simvastatin, (65+2.6)×10<sup>-3</sup> g·kg<sup>-1</sup>] group. Another 10 MKR mice of the same age were classified into the blank group and 10 FVB mice into the normal group. After eight weeks of intragastric administration in each group,the liver wet weight,oral glucose tolerance test (OGTT),serum inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>),and changes in blood lipid and liver function were determined. Hematoxylin-eosin (HE) staining was conducted for observing the morphological changes in liver tissue under a transmission electron microscope,followed by the detection of Toll-like receptor 4 (TLR4),myeloid differentiation factor 88 (MyD88),and nuclear transcription factor-<italic>κ</italic>B (NF-<italic>κ</italic>B) protein expression by Western blot. Result:Compared with the model group,the medication groups exhibited significantly reduced liver wet weight index (<italic>P</italic><0.01),improved OGTT result (<italic>P</italic><0.05),and down-regulated serum IL-6 and TNF-<italic>α</italic> levels (<italic>P</italic><0.01). In terms of morphological changes,Yinchenhao Tang protected the hepatocyte structure and alleviated hepatocyte steatosis. Moreover, Yinchenhao Tang obviously down-regulated the protein expression levels of TLR4,MyD88,and NF-<italic>κ</italic>B in liver tissue of MKR mice with T2DM combined with NAFLD (<italic>P</italic><0.05),and the down-regulation of TLR4 and NF-<italic>κ</italic>B in the original Yinchenhao Tang group was better than that in the Yinchenhao Tang group at a specified dose in teaching materials (<italic>P</italic><0.05). Conclusion:Yinchenhao Tang is able to reduce inflammatory factor levels and down-regulate TLR4,MyD88,and NF-<italic>κ</italic>B expression in liver tissue to relieve the pathological liver injury and interfere with T2DM combined with NAFLD of MKR mice. It exerts a certain liver-protective effect by lowering the blood lipids and delaying the hepatic inflammation.

3.
Article in Chinese | WPRIM | ID: wpr-882382

ABSTRACT

Dyslipidemia is a risk factor for stroke, and reducing low-density lipoprotein cholesterol (LDL-C) levels can decrease the risk of ischemic stroke. Both statins and PCSK9 inhibitors can effectively reduce LDL-C levels, but some patients cannot tolerate statins, and PCSK9 inhibitors are expensive. Animal experiments have shown that ezetimibe can reduce oxidative stress and endoplasmic reticulum stress response, inhibit cell apoptosis, increase autophagy, and protect nerve tissue by activating AMP activated protein kinase (AMPK) signaling pathway. Clinical studies have shown that ezetimibe can further reduce LDL-C levels on the basis of statin therapy, and reduce the risk of ischemic stroke in patients with acute coronary syndromes, and there is no obvious adverse reaction. However, ezetimibe cannot reduce the incidence of stroke in elderly people who are ≥75 years old, have elevated LDL-C and do not have coronary heart disease. At present, there are few studies on the use of ezetimibe for secondary prevention of ischemic stroke, and its effect needs to be further confirmed by large randomized controlled trials.

4.
Asian Journal of Andrology ; (6): 602-610, 2021.
Article in English | WPRIM | ID: wpr-922364

ABSTRACT

Low-intensity pulsed ultrasound (LIPUS) is a promising therapy that has been increasingly explored in basic research and clinical applications. LIPUS is an appealing therapeutic option as it is a noninvasive treatment that has many advantages, including no risk of infection or tissue damage and no known adverse reactions. LIPUS has been shown to have many benefits including promotion of tissue healing, angiogenesis, and tissue regeneration; inhibition of inflammation and pain relief; and stimulation of cell proliferation and differentiation. The biophysical mechanisms of LIPUS remain unclear and the studies are ongoing. In recent years, more and more research has focused on the relationship between LIPUS and stem/progenitor cells. A comprehensive search of the PubMed and Embase databases to July 2020 was performed. LIPUS has many effects on stem cells. Studies show that LIPUS can stimulate stem cells in vitro; promote stem cell proliferation, differentiation, and migration; maintain stem cell activity; alleviate the problems of insufficient seed cell source, differentiation, and maturation; and circumvent the low efficiency of stem cell transplantation. The mechanisms involved in the effects of LIPUS are not fully understood, but the effects demonstrated in studies thus far have been favorable. Much additional research is needed before LIPUS can progress from basic science research to large-scale clinical dissemination and application.


Subject(s)
Cell Proliferation , Humans , Signal Transduction , Stem Cells/radiation effects , Ultrasonic Therapy/methods , Ultrasonic Waves
5.
Article in Chinese | WPRIM | ID: wpr-864713

ABSTRACT

Objective:To explore the latent classes in developmental trajectory of post traumatic growth(PTG) and influencing factors in patients with bladder cancer.Methods:Convenience sampling method was used to recruit 215 patients with bladder cancer underwent transurethral resection or radical cystectomy in the Urological Department of Hubei Cancer Hospital. The patients were followed up for 5 times, and the latent growth mixture model and multinomial Logistic regression model were used to analyze the data.Results:The patients were identified four latent classes: high PTG group(29 cases), low PTG group(33 cases), increasing PTG group(69 cases), decreasing PTG group(84 cases). Compared with the low PTG group, elderly, patients with non-muscle invasive bladder cancer were more likely to enter the high PTG group. Patients with non-muscle invasive bladder cancer, male and employee were more likely to enter the increasing PTG group. Patients living in rural areas were more likely to enter the decreasing PTG group.Conclusions:Clinical staff should make individualized intervention according to the developmental trajectory of PTG in patients with bladder cancer.

6.
Chinese Journal of Biotechnology ; (12): 2610-2621, 2020.
Article in Chinese | WPRIM | ID: wpr-878515

ABSTRACT

Strain is the fundamental unit in microbial taxonomy. The functional diversity among strains has great influence on host phenotypes. With the development of microbiome research, knowing the composition and functional capacities of complex microbial communities at the strain level has become increasingly valuable in scientific research and clinical applications. This review introduces the principles of bioinformatics algorithms for strain analysis based on metagenomic data, the applications in microbiome research and directions of future development.


Subject(s)
Algorithms , Computational Biology , Metagenome , Metagenomics , Microbiota/genetics
7.
Article in Chinese | WPRIM | ID: wpr-876179

ABSTRACT

Objective To identify the epidemiological features of COVID-19 epidemic in Chenzhou City, Hunan province so as to provide scientific evidence for effective containment of the COVID-19 epidemic. Methods Descriptive epidemiological method was used to retrospectively characterize the confirmed COVID-19 cases and asymptomatic cases in Chenzhou City from January 23 through March 10, 2020. Results A total of 39 confirmed COVID-19 cases and 6 asymptomatic cases infection were documented in the city, with no death.We identified 8 clusters of COVID-19, which were all familial transmission.There was statistical difference between the sources of different types of epidemic (χ2=15.996, P < 0.001), in which all the local COVID-19 cases were the secondary cases in the clusters.As the epidemic expanded, the trend shifted from imported-case-centered to local-case-centered.The epidemic has covered 81.81% of the city area; in each area, the first COVID-19 cases were all imported. Conclusion The COVID-19 epidemic has been effectively controlled.At present, we focus on the quarantine in the entry and exit to prevent the imported epidemic.

8.
Article in Chinese | WPRIM | ID: wpr-905643

ABSTRACT

Objective:To compare the effect of two kinds of methods inducing ankle dorsiflexion on ankle dorsiflexion function for stroke patients. Methods:From September, 2016 to September, 2018, 60 patients with disorders of ankle active dorsiflexion after stroke were randomly divided into groups A, B and C, who accepted routine rehabilitation, tapping-zone therapy and tapping Qiuxu acupoint (GB40), respectively, for six weeks. They were assessed with three-dimensional gait analysis and surface electromyography before and after treatment. Results:The range of motion of the affected ankle, the peak torque of ankle and integrated electromyography of tibialis anterior muscle increased after treatment (t > 2.318, P < 0.05), and it ranked from best to worst as group C, group B and group A (P < 0.05). Conclusion:Both tapping-zone therapy and tapping Qiuxu may promote the recovery of ankle dorsiflexion in stroke patients, and the latter seems better.

9.
National Journal of Andrology ; (12): 72-76, 2019.
Article in Chinese | WPRIM | ID: wpr-818827

ABSTRACT

Decreased sperm count, low sperm motility and sperm malformation are important factors of male infertility, but their pathogenic mechanisms have not yet been elucidated. Autophagy is an evolutionarily highly conservative cellular process and plays an important role in spermatogenic cells in both normal physiological and adverse conditions. On the one hand, autophagy can degrade the majority of long-lived proteins and organelles to maintain the intracellular homeostasis of spermatogenic cells, the meiosis of spermatocytes and spermiogenesis, and improve sperm motility. On the other hand, excessive autophagy can lead to excessive consumption of proteins and damage to organelles, induce cellular dysfunction, and result in sperm count reduction, spermatogenic defects, and low sperm motility. This review presents an overview of the recent studies on the influences of autophagy on spermatogenesis and sperm motility, aiming for some new therapeutic targets for male infertility.

10.
Journal of Medical Postgraduates ; (12): 263-267, 2019.
Article in Chinese | WPRIM | ID: wpr-818224

ABSTRACT

Objective Vibration response imaging (VRI) has been applied to the bedside monitoring of critically ill patients. The purpose of this study was to explore the value of VRI in assessing lung recruitment in patients with acute respiratory distress syndrome (ARDS). Methods We prospectively studied the clinical data on 20 cases of ARDS treated in our Department of Pulmonary and Critical Care Medicine from January 2015 to June 2017. The positive end-expiratory pressure (PEEP) of the patients was increased from 5 and 15 cm H2O, the mean grey value of the max energy frame of VRI was determined, the quantitative lung data (QLD) were obtained, and the correlation of the VRI image with the recruited lung volume and oxygenation index was analyzed. Results The patients with PEEP at 15 cm H2O, in comparison with those with PEEP at 5 cm H2O, showed a significantly decreased mean gray value of the max energy frame of VRI (169.1 ± 11.3 vs 175.1 ± 15.9, P = 0.04), increased gray area ([56.3 ± 4.4]% vs [52.7 ± 7.5]%, P < 0.05), declined QLD in the upper left and left middle regions (P < 0.05) and elevated in the lower left and lower right regions (P < 0.05). With the PEEP at 15 cm H2O, the mean gray value of VRI was increased by -5.6 ± 12.8, negatively correlated with the recruited lung volume (r = -0.785, P < 0.01), and the gray area increased (3.8 ± 4.8)%, positively correlated with the recruited lung volume (r =0.793, P < 0.01). With PEEP at 5 and 15 cm H2O, the oxygenation indexes were (116.3 ± 25.6) mmHg and (116.3 ± 25.6) mmHg, respectively, the improvement rate of which correlated negatively with the increased mean gray value of VRI (r = -0.740, P < 0.01) but positively with the gray area (r = 0.581, P < 0.01). Conclusion Lung recruitment can be adequately estimated with bedside VRI in patients with ARDS.

11.
Article in English | WPRIM | ID: wpr-776834

ABSTRACT

Non-small cell lung cancer (NSCLC) accounts for 80%-85% of all lung malignancies and good diagnosis and prognosis of NSCLC are critical to the increase of its survival rate. Tumor-associated macrophages (TAM) abundantly present in numerous cancer types, and the role of TAMs in tumor biology and their prognostic value in cancer become major topics of interest. After various stimulations in the tumor microenvironment, TAMs develop into a M1 (tumor-inhibitory) phenotype or M2 (tumor-promoting) phenotype. Recent studies show that traditional Chinese medicine (TCM) monomers have markedly inhibitory actions for NSCLC through M1/M2 modulation. Due to the TCM monomers mainly covered five categories, i.e. terpenoids, flavonoids, polysaccharides, natural polyphenols, and alkaloids. Thus, we will discuss the regulation of TCM monomers on TAM involve in these five parts in this review. In addition, the potential role of TAMs as therapeutic targets will be discussed.

12.
Article in Chinese | WPRIM | ID: wpr-776662

ABSTRACT

OBJECTIVE@#To investigate the incidence of neonatal asphyxia and possible contributing factors for the development of severe asphyxia in Hubei Enshi Tujia and Miao Autonomous Prefecture, China.@*METHODS@#A total of 16 hospitals in Hubei Enshi Tujia and Miao Autonomous Prefecture were selected as research centers. A retrospective analysis was performed for the clinical data of 22 294 live births in these 16 hospitals from January to December, 2016 to investigate the incidence rate of neonatal asphyxia and possible contributing factors for the development of severe asphyxia.@*RESULTS@#Of the 22 294 neonates born alive, 733 (3.29%) were diagnosed with neonatal asphyxia, among whom 627 had mild asphyxia and 106 had severe asphyxia. The neonates with low maternal education level, maternal anemia during pregnancy, chorioamnionitis, abnormal amniotic fluid, abnormal umbilical cord, placenta previa, placental abruption, Tujia Minority, preterm birth, and low birth weight had a higher incidence of severe asphyxia (P<0.05).@*CONCLUSIONS@#The incidence rate of neonatal asphyxia in Hubei Enshi Tujia and Miao Autonomous Prefecture is higher. Low maternal education level, maternal anemia during pregnancy, chorioamnionitis, abnormal amniotic fluid, abnormal umbilical cord, placenta previa, placental abruption, Tujia Minority, preterm birth, and low birth weight may be related to the development of severe neonatal asphyxia.


Subject(s)
Asphyxia Neonatorum , Epidemiology , China , Humans , Incidence , Infant, Newborn , Retrospective Studies
13.
Article in Chinese | WPRIM | ID: wpr-806642

ABSTRACT

Objective@#To analyze the clinical manifestations and results of etiological examinations of 17 elderly patients with influenza A (H1N1) viral pneumonia, and to understand the clinical features of pneumonia and molecular characteristics of influenza A (H1N1) virus infection in the elderly.@*Methods@#The elderly patients with pneumonia who were hospitalized in the Department of Respiratory Diseases of Nanjing First Hospital from January 2018 to March were enrolled. The cases were confirmed by nucleic acid examination for influenza virus and the clinical data were collected. After the amplification of the whole genome of influenza virus, the high throughput sequencing and bioinformatics analysis were performed.@*Results@#The mean age of the 17 enrolled patients was 73.8±10.8. All of them had at least 1 underlying disease, and 7 cases had co-infection. Respiratory symptoms and fever were the most prominent clinical manifestations. Lesions in both lungs were found in 76.5% of the patients. The result of high throughput sequencing showed that all the viruses were highly homologous to the vaccine strain, and the HA gene belonged to the 6B.1 subgroup. Furthermore, three variations of antigenic locus (H138Y, S74R and S164T in HA) and a drug-resistant variation (H275Y in NA) were detected in the circulating strains.@*Conclusions@#Elderly patients with influenza A (H1N1) virus pneumonia often have underlying diseases and are prone to have co-infection. The molecular characteristics of the virus and the variation of key amino acid loci should be closely monitored in order to provide evidence for epidemic prevention and clinical antiviral treatment.

14.
Chinese Journal of Epidemiology ; (12): 770-775, 2018.
Article in Chinese | WPRIM | ID: wpr-738044

ABSTRACT

Objective To investigate the association between maternal pre-pregnant body mass index and gestational weight gain,as well as their interaction on neonatal birthweight.Methods We built a cohort in Anqing Municipal Hospital from January 2014 to March 2015,enrolling pregnant women who decided to give birth in this hospital.All women were asked to fill a questionnaire for basic information collection.Medical information of both pregnant women and their newborns were obtained through electronic medical record.Chi-square analysis,multinomial logistic regression,multiplicative and additive interaction methods were used to analyze the association between prepregnant body mass index and gestational weight gain as well as their interactions on birth weight of the neonates.Results A total of 2 881 pregnant women were included in this study.Of the 2 881 newborns,359 (12.46%) were small for gestational age (SGA) and 273 (9.48%) were large for gestational age (LGA).After adjusting the possible confounding factors,results from the multinomial logistic regression showed that pre-pregnancy underweight women were more possible to deliver SGA (aRR=1.33,95%CI:1.02-1.73).If the gestational weight gain was below the recommended criteria,the risk of SGA (aRR=1.64,95% CI:1.23-2.19) might increase.Pre-pregnancy overweight/obese could increase the risk of being LGA (aRR=1.86,95% CI:1.33-2.60).Maternal gestational weight gain above the recommendation level was associated with higher rates of LGA (aRR=2.03,95%CI:1.49-2.78).Results from the interaction analysis showed that there appeared no significant interaction between pre-pregnancy BMI and gestational weight on birthweight.Conclusion Pre-pregnancy body mass index and gestational weight gain were independently associated with neonatal birthweight while pre-pregnancy BMI and gestational weight gain did not present interaction on birthweight.

15.
Chinese Journal of Epidemiology ; (12): 770-775, 2018.
Article in Chinese | WPRIM | ID: wpr-736576

ABSTRACT

Objective To investigate the association between maternal pre-pregnant body mass index and gestational weight gain,as well as their interaction on neonatal birthweight.Methods We built a cohort in Anqing Municipal Hospital from January 2014 to March 2015,enrolling pregnant women who decided to give birth in this hospital.All women were asked to fill a questionnaire for basic information collection.Medical information of both pregnant women and their newborns were obtained through electronic medical record.Chi-square analysis,multinomial logistic regression,multiplicative and additive interaction methods were used to analyze the association between prepregnant body mass index and gestational weight gain as well as their interactions on birth weight of the neonates.Results A total of 2 881 pregnant women were included in this study.Of the 2 881 newborns,359 (12.46%) were small for gestational age (SGA) and 273 (9.48%) were large for gestational age (LGA).After adjusting the possible confounding factors,results from the multinomial logistic regression showed that pre-pregnancy underweight women were more possible to deliver SGA (aRR=1.33,95%CI:1.02-1.73).If the gestational weight gain was below the recommended criteria,the risk of SGA (aRR=1.64,95% CI:1.23-2.19) might increase.Pre-pregnancy overweight/obese could increase the risk of being LGA (aRR=1.86,95% CI:1.33-2.60).Maternal gestational weight gain above the recommendation level was associated with higher rates of LGA (aRR=2.03,95%CI:1.49-2.78).Results from the interaction analysis showed that there appeared no significant interaction between pre-pregnancy BMI and gestational weight on birthweight.Conclusion Pre-pregnancy body mass index and gestational weight gain were independently associated with neonatal birthweight while pre-pregnancy BMI and gestational weight gain did not present interaction on birthweight.

16.
Chinese Journal of Geriatrics ; (12): 197-201, 2018.
Article in Chinese | WPRIM | ID: wpr-709219

ABSTRACT

Objective To explore the effect of combined Naikan therapy and modified Morita therapy on psychological distress and post traumatic growth in elderly patients with cancer pain.Methods Ninety elderly patients with cancer pain were randomly assigned into two groups;those in the study group(n =45)were given Naikan therapy and modified Morita therapy for 4 weeks and those in the control group (n =45) were given standardized aerodyne treatment and standardized nursing care.All subjects were assessed with the Distress Thermometer and Problem Listand the Post traumatic Growth Inventory before and after Naikan therapy and modified Morita therapy.Results Compared with the control group,the study group was associated with significantly decreased scores of psychological distress(1.8± 0.1 vs.3.9 ± 0.2,t =1.78,P<0.05),emotional problems (1.2 ± 0.4,vs.2.4±0.4,t=1.41,P<0.05)and family problems(1.1±0.1,vs.2.9±0.1,t=1.63,P<0.05).The study group also showed markedly higher scores in posttraumatic growth(66.0± 19.9 vs.45.3± 27.6,t=2.58,P<0.05),relationships to others(34.8±12.1 vs.23.8±12.2,t=1.91,P<0.05),new possibilities(25.2 ± 10.1 vs.13.7± 4.4,t=1.94,P<0.05),personal strength(20.7 ± 10.4 vs.7.6 ± 3.1,t =2.03,P < 0.05),spiritual change (11.6 ± 5.6 vs.5.4 ± 2.7,t =1.26,P < 0.05),and appreciation of life(18.9±6.2 vs.6.1±-2.1,t=1.88,P<0.05) than the control group.Conclusions Naikan therapy and modified Morita therapy can decrease psychological distress and improve post traumatic growth in elderly patients with cancer pain.

17.
The Journal of Practical Medicine ; (24): 406-409,415, 2018.
Article in Chinese | WPRIM | ID: wpr-697626

ABSTRACT

Objective To study the effect of transcutaneous electroacupuncture(TEA)on gastric accom-modation and electrogastrogram in patients with functional dyspepsia(FD)and investigate the transcutaneous elec-troacupuncture efficacy and mechanism. Methods 106 cases of FD patients were randomLy divided into TEA group(n=53)and sham-TEA group(n=53)according to the designed random table. 10 health volunteers were recruited for participation in the study for normal control group. All patients fulfilled the Rome Ⅲ criteria of FD. Informed consent was obtained from each participant. All subjects were tested in terms of gastric accom-modation (GA),namely,maximum satiety. For the patients of TEA group,the acupoints of Zusanli(ST36)and Neiguan (PC6)were stimulated. In the sham-TEA group,the sham-acupoint for PC6 was located at about 15-20 cm away from PC6 not on any meridian and the sham-point for ST36 was located at 10-15cm down from and to the lateral side of ST36 not on any meridian.The stimulation parameters used for sham-TEA were the same as the TEA group. Four weeks after treatment with double blind crossover method,we compared the two groups in view of gastric receptivity,electrogastrogram(EGG)andclinicalsymptomscores before and after treatment respectively. Results (1)The GA was 725 ± 46 mL in the normal control group and 539 ± 36 mL in the FD patients.(2)The mean total symptom score was 24.5 ± 2.9 at baseline and significantly decreased to 11.9 ± 2.1(P < 0.001)after TEA. There were significant differences in the symptom scores between the TEA group and sham-TEA group(P<0.001).(3) The percentage of normal slow waves(N%)during fasting and postprandial state in the TEA group were respectively 70.9 ± 2.4% and 68.2 ± 3.1%. The differences were statistically significant between the TEA group,baseline and sham-TEA group(P<0.001).(4)In the TEA group,the postprandial dominant power(46.23 ± 4.03)db and the fasting dominant power(35.35 ± 1.53)db were significantly increased compared to baseline before treatment and sham-TEA group(P < 0.001). Conclusions(1)Gastric accommodation in patients with FD was decreased, showing a higher visceral sensitivity. TEA can improve the GA in FD patients,reducing visceral sensitivity.(2)TEA can increase the percentage of normal slow wave in the FD patients,increase the postprandial dominant power and regulate the abnormal gastric myoelectric activity to promote the gastric motility.

18.
Article in Chinese | WPRIM | ID: wpr-696167

ABSTRACT

Objective To explore serum long stranded noncoding RNA (lncRNA) transcript 1 (PCAT-1) expression level of patients with multiple myeloma (MM) and clinical value.Methods 72 cases of patients with MM treated in the Second People's Hospital of Zhaoqing City were selected as the study objects,and 60 cases of normal subjects undergoing physical examination in the same period were as the control group.Serum lncRNA PCAT-1 expression was detected by RT-PCR method.The relationship between lncRNA PCAT 1 expression and clinical pathological parameters,treatment effect was analyzed,and 5 years survival was analyzed by using Kaplan-Meier,and survival difference was detected by using Log-Rank method.Results Serum PCAT-1mRNA expression in MM group (2.65 ± 0.64) was significantly higher than that in the control group (1.06 ± 0.23,t=18.276,P=0.000).There were no significant differences in sex,clinical stage and pathological types of hemoglobin,plasma cells,platelets,albumin,β2-MG and CRP between PCAT 1 mRNA high expression group and low expression group (x2 =0.001 ~ 3.345,all P > 0.05).Ca2+ ≥ 10 mg/dl in the PCAT-1 high expression group (57.14%) was significantly higher than that in the low expression group (27.27%,x2 =5.229,P=0.022).There was no significant difference in treatment effect between PCAT-1 mRNA high expression group and low expression group (88.64 % vs 75.00%,x2 =2.291,P=0.130).PFS and OS expression in PCAT-1 high expression group were lower than that in the low expression group (x2 =7.269,P =0.007;x2 =9.190,P =0.002).COX risk regression multiple factor analysis showed that age and PCAT-1mRNA expression were independent prognostic factors influencing patients (OR =3.275,P =0.025,95%CI:2.691~3.761;OR=2.136,P=0.046,95%CI:2.034~2.685).Conclusion LncRNA PCAT-1 is highly expressed in serum of patients with multiple myeloma,and correlated with the prognosis of patients.

19.
Chinese Pharmaceutical Journal ; (24): 1098-1103, 2018.
Article in Chinese | WPRIM | ID: wpr-858288

ABSTRACT

OBJECTIVE: To establish a high performance liquid chromatography mass spectrometry (HPLC-MS/MS) method to determine the cinacalcet concentration in plasma of dialysis patients. METHODS: Fendiline hydrochloride was selected as the internal standard (IS), in the meantime, plasma samples were extracted through the solid phase extraction (SPE), and the internal standard method was used to determine the concentration of cinacalcet. The analyte was separated by Inertsil SIL-150A (2.1 mm×50 mm, 5 μm) matched with guard column Inertsil SIL-150A (3.0 mm×50 mm,5 μm). The cinacalcet was eluted with acetonitrile, water and formic acid (90:10:1=V:V:V) at a rate of 0.35 mL•min-1. On MS, electrospray ionization (ESI) source with positive ion mode and multiply reaction monitoring (MRM) was used. The MRM transitions of cinacalcet and the IS were 358.1→155.1 and 316.0→212.1, respectively. The concentration in dialysis patients after 25 mg dose (one piece of tablet) within 24 h was determined by using the established HPLC-MS/MS method in this paper. RESULTS: A good linearity was obtained in the range of 0.1-50 ng•mL-1 of cinacalcet, and the validated accuracy and precision all were within ±15%. Long-term, freeze-thaw and autoinjector stability of cinacalcet QC samples were all in the range of 1.4%-3.9%. The matrix of cinacalcet is (104.5±3.1)%, (105.6±4.3)% and (104.0±3.1)% respectively and the matrix of IS is (106.4±3.0)%. This validated method can meet the guidance for bioanalytical method validation. CONCLUSION: The HPLC-MS/MS method is sensitive and specific enough to meet the quantitative requirements for determining cinacalcet in human plasma. It is rapid, accurate and in line with the guidance for bioanalytical method validation.

20.
Article in Chinese | WPRIM | ID: wpr-923746

ABSTRACT

@#Objective To explore an accurate, comprehensive and easy-to-operate assessment of lower limbs exercise capacity for the football players. Methods From June, 2012 to June, 2015, seven healthy young football players (healthy group) and their seven teammates after thighs muscle strain returning to play (injured group) were measured the synergism constriction pattern, activation time and the degree of muscle activation of related muscle during single leg jump, with NORAXON, a 16-channel wireless electromyography telemetry system.Results The sequence of force was significant difference between the two groups (t>2.223, P<0.05). The contribution of long head of biceps was more in the injured group (t=2.778, P<0.05).Conclusion The sequence of muscle force in movement varies after lower extremities injury in athletes. Surface electromyography can test the weakness of local muscle of athletes, to help the evaluation and rehabilitation for them.

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