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1.
Cancer Research and Clinic ; (6): 414-418, 2021.
Article in Chinese | WPRIM | ID: wpr-912898

ABSTRACT

Objective:To investigate the correlation between the number of circulating tumor cells (CTC) in peripheral blood and clinicopathological features of patients with breast cancer.Methods:The clinical data of 104 breast cancer patients at Guangzhou Panyu Central Hospital between January 2017 and May 2020 were retrospectively analyzed. The number of CTC in peripheral blood, the levels of serum tumor markers [alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), carbohydrate antigen (CA)125, CA153] were detected. In blood samples, the number of CTC ≥ 2/ml was defined as CTC positive. Immunohistochemistry was used to analyze the protein expression of Ki-67 in tumor tissues. The association of CTC with clinicopathological features, serum tumor markers and Ki-67 protein expression was also analyzed.Results:The CTC positive rate was 80.77% (84/104). There were statistically significant differences in composition of whether there was vascular tumor thrombus (χ 2 = 0.860, P = 0.009), axillary lymph node metastasis (χ 2 = 12.382, P<0.01), N staging ( P = 0.002) and TNM staging (χ 2 = 7.698, P = 0.006) between patients with CTC positive and negative. However, there were no statistically significant differences in composition of age ( t = 0.634, P = 0.528), tumor quadrant (χ 2 = 6.523, P = 0.163), molecular subtyping (χ 2 = 4.164, P = 0.384), histological grade (χ 2 = 1.901, P = 0.387), T staging ( P = 0.099) and whether there was nerve invasion (χ 2 = 0.092, P = 0.761). The levels of serum CEA and CA125 in CTC positive patients were higher than those in CTC negative patients [median ( P25, P75): 2.50 ng/ml (2.21 ng/ml, 2.92 ng/ml) vs. 1.89 ng/ml (1.61 ng/ml, 2.35 ng/ml); 13.81 U/ml (11.79 U/ml, 16.28 U/ml) vs. 11.17 U/ml (8.91 U/ml, 12.80 U/ml); all P < 0.05], and CTC was positively correlated with serum CEA and CA153 levels ( r = 0.520, P<0.01; r = 0.497, P<0.01); CTC was not related to Ki-67 protein expression (χ 2 = 0.512, P = 0.474). Conclusion:The number of CTC in peripheral blood is closely related to clinical staging, lymph node or hematogenous metastasis, tumor markers CEA and CA153 levels of breast cancer. The increased number of CTC may cause tumor progression and metastasis.

2.
Article in Chinese | WPRIM | ID: wpr-912344

ABSTRACT

Objective:To analyze the risk factors for postoperative pulmonary venous obstruction after correction of total anomalous pulmonary venous connection(TAPVC).Methods:We retrospectively reviewed all patients undergoing operative repair of TAPVC in our institution from December 2013 to January 2018. Patients with functionally univentricular circulations or atrial isomerism were excluded. Patients were divided into two groups according to whether there was pulmonary vein obstruction. The clinical variables of the two groups were compared. Variables for the multivariable analysis were chosen if there was statistical significance on univariable analysis.Results:145 patients were included, 91(63%) males, aged 4(2, 8)months and weight 5.5(4.5, 7.5)kg. Mean follow-up interval was(51±23) months. Postoperative obstruction developed in 27 patients(18.6%). The differences of anatomic type[supracardiac 18(67%) vs.59(50%), cardiac 4(15%) vs. 50(42%), infracardiac 3(11%) vs. 1(1%), mixed 2(7%) vs. 8(7%), P=0.003], preoperative obstruction[yes 19(70%) vs. 37(31%), no 8(30%) vs. 81(69%), P<0.001], associated cardiac lesions[yes 13(48%) vs. 27(23%), no 14(52%) vs. 91(77%), P=0.008] and bypass time[109(89, 129)min vs. 88(70, 110)min, P=0.002] between two groups were statistical significant. A multivariable model showed preoperative obstruction( P<0.001) and bypass time( P=0.009) were associated with postoperative obstruction. Conclusion:The incidence of pulmonary vein obstruction after correction of TAPVC was still high. If there was preoperative obstruction, or the bypass time was too long during operation, the surveillance of pulmonary vein obstruction should be strengthened after operation.

3.
Article in Chinese | WPRIM | ID: wpr-907133

ABSTRACT

With the advent of combination antiretroviral therapy (cART), the life expectancy of HIV-infected individuals is increasing. As the result, more HIV-infected individuals are facing several chronic diseases including cardiovascular diseases (CVD). Hypertension is one of the most important traditional CVD risk factors in HIV-infected individuals. It is of importance to make effective prevention and treatment of hypertension to decrease the morbidity and mortality of cardiovascular disease. In this paper, a literature retrieval was conducted by using PubMed, CNKI, WANFANG and VIP databases for studies on the influence factors and prevalence of hypertension in HIV-infected individuals from 2009 to October 2020. Finally, 57 articles were included. This review aims to provide evidence for further research on the influence factors and prevalence of hypertension in HIV-infected individuals.

4.
Article in Chinese | WPRIM | ID: wpr-907125

ABSTRACT

ObjectiveTo understand the infection of AIDS, syphilis, and gonorrhea in men who have sex with men (MSM), and the factors influencing the acceptance of gonorrhea testing in Dehong Dai Jingpo Autonomous Prefecture (Dehong Prefecture), Yunnan Province. MethodsAcross-sectional survey was conducted to describe and analyze the acceptance of AIDS, syphilis, and gonorrhea testing among MSM tracked in Dehong Prefecture in 2018. ResultsA total of 385 MSM were included in the analysis, with a 100.0% HIV and syphilis testing rate. The HIV antibody positivity rate was 10.6% (41/385) and the positivity rate of syphilis antibody was 14.8%(57/385). Only 30.4% of the subjects were willing to test for gonorrhea, and 13.7%(16/117)of them were test positive. Further multifactorial analysis revealed that local MSM and syphilis co-infected individuals were more likely to be tested for gonorrhea (aOR=2.48, 95%CI=1.33-4.65, P=0.004; aOR=2.59, 95%CI=1.43-4.71, P=0.002). ConclusionThe positive rates of AIDS, syphilis and gonorrhea in MSM population in Dehong Prefecture are relatively high. Integrated interventions of STD/AIDS and gonorrhoea detection should be strengthened.

5.
Article in Chinese | WPRIM | ID: wpr-907110

ABSTRACT

With the advent of combination antiretroviral therapy (cART), the life expectancy of HIV-infected individuals is increasing. As the result, more HIV-infected individuals are facing several chronic diseases including cardiovascular diseases (CVD). Hypertension is one of the most important traditional CVD risk factors in HIV-infected individuals. It is of importance to make effective prevention and treatment of hypertension to decrease the morbidity and mortality of cardiovascular disease. In this paper, a literature retrieval was conducted by using PubMed, CNKI, WANFANG and VIP databases for studies on the influence factors and prevalence of hypertension in HIV-infected individuals from 2009 to October 2020. Finally, 57 articles were included. This review aims to provide evidence for further research on the influence factors and prevalence of hypertension in HIV-infected individuals.

6.
Article in Chinese | WPRIM | ID: wpr-907102

ABSTRACT

ObjectiveTo understand the infection of AIDS, syphilis, and gonorrhea in men who have sex with men (MSM), and the factors influencing the acceptance of gonorrhea testing in Dehong Dai Jingpo Autonomous Prefecture (Dehong Prefecture), Yunnan Province. MethodsAcross-sectional survey was conducted to describe and analyze the acceptance of AIDS, syphilis, and gonorrhea testing among MSM tracked in Dehong Prefecture in 2018. ResultsA total of 385 MSM were included in the analysis, with a 100.0% HIV and syphilis testing rate. The HIV antibody positivity rate was 10.6% (41/385) and the positivity rate of syphilis antibody was 14.8%(57/385). Only 30.4% of the subjects were willing to test for gonorrhea, and 13.7%(16/117)of them were test positive. Further multifactorial analysis revealed that local MSM and syphilis co-infected individuals were more likely to be tested for gonorrhea (aOR=2.48, 95%CI=1.33-4.65, P=0.004; aOR=2.59, 95%CI=1.43-4.71, P=0.002). ConclusionThe positive rates of AIDS, syphilis and gonorrhea in MSM population in Dehong Prefecture are relatively high. Integrated interventions of STD/AIDS and gonorrhoea detection should be strengthened.

7.
Article in Chinese | WPRIM | ID: wpr-906538

ABSTRACT

Hydroxytyrosol as one of natural anti-oxidants,extracted from the fruits and leaves of Olea europaea,is a natural polyphenol compound in the form of esters. Recently,considerable studies showed that hydroxytyrosol demonstrated intrinsic biological activity for metabolic syndromes, cardiovascular- and neurodegenerative-related diseases,and it was revealed to play the roles in the anti-activities of cancerous,inflammatory as well as depressing issues. In addition,hydroxytyrosol is an oleophilic and hydrophilic compound with high bioavailability and low cellular cytotoxicity. It could be absorbed by various tissues and could easily passe through blood brain barrier. Therefore,hydroxytyrosol was introduced as one of the key subjects targeted by innovative drug development. However,it has a short half-life in vivo and non-tissue specific,which lead to its limitation in clinical application, so further in-depth studies are still needed. The authors had a literature review of hydroxytyrosol,and summarized the basic properties of its pharmacokinetic,pharmacological effects and molecular mechanisms. This article mainly focused on it’s pharmacological activity and the mechanism involved in treating damages induced by the oxidative stress,in alleviating cardiovascular diseases and in inhibition of neurodegenerative diseases. In this article, its anti-inflammatory,anti-tumor,anti-depressant effects,other biological activities,and pharmacokinetics were also briefly reviewed. The authors put forward some personal thoughts on its future research direction,hoping to provide ideas and inspirations for the vast number of researchers,and provide references for its further development,research and application.

8.
Article in Chinese | WPRIM | ID: wpr-906420

ABSTRACT

Objective:To explore the effects and mechanism of Chinese classical prescription Dahuang Zhechongwan on silicosis in mice. Method:Thirty-six male Kunming mice of SPF grade were randomized into the normal control group, model control group, tetrandrine (Tet, 0.039 mg·kg<sup>-1</sup>) group, as well as high- (1.560 g·kg<sup>-1</sup>), medium- (0.780 g·kg<sup>-1</sup>), and low-dose (0.390 g·kg<sup>-1</sup>) Dahuang Zhechongwan groups, with six mice in each group. Mice in all groups except for the normal control group underwent static inhalation of silica (SiO<sub>2</sub>) dust for 40 consecutive days to induce fibrosis. After 28 days of intervention with corresponding drugs, the mice were sacrificed to collect the serum and lung tissues, with the former used for detecting tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>), interleukin-1<italic>β </italic>(IL-1<italic>β</italic>), IL-6, and hydroxyproline (HYP) levels by enzyme-linked immunosorbent assay (ELISA) and the latter for observing the pathological changes. Meanwhile, the protein and mRNA expression levels of p38 mitogen-activated protein kinase (p38 MAPK), nuclear transcription factor-<italic>κ</italic>B (NF-<italic>κ</italic>B), transforming growth factor-<italic>β</italic><sub>1</sub> (TGF-<italic>β</italic><sub>1</sub>), <italic>α</italic>-smooth muscle actin (<italic>α</italic>-SMA), Smad2, Smad3, and Smad7 in the lung tissues were determined by Western blot and real-time polymerase chain reaction (Real-time PCR). Result:Compared with the normal group, the contents of TNF-<italic>α</italic>, IL-1<italic>β</italic>, IL-6 and HYP in the model group were significantly increased, the difference was statistically significant(<italic>P</italic><0.05,<italic>P</italic><0.01); compared with the model group, the high-dose group of Dahuang Zhechongwan could significantly reduce the contents of TNF-<italic>α</italic>, IL-6 and HYP in the serum of mice(<italic>P</italic><0.05, <italic>P</italic><0.01), indicating that Dahuang Zhechongwan could reduce the lung inflammation of silicosis mice. At the same time, compared with the normal group, the protein and mRNA expression levels of p38 MAPK, NF-<italic>κ</italic>B p65, TGF-<italic>β</italic><sub>1</sub>, <italic>α</italic>-SMA, Smad2 and Smad3 in the model group were significantly increased(<italic>P</italic><0.05,<italic>P</italic><0.01), while the protein and mRNA expression levels of Smad7 were significantly decreased(<italic>P</italic><0.01); compared with the model group, the protein and mRNA expression levels of p38 MAPK, NF-<italic>κ</italic>B p65, TGF-<italic>β</italic><sub>1</sub>, <italic>α</italic>-SMA, Smad2 and Smad3 in the high-dose Dahuang Zhechongwan group were significantly increased the protein and mRNA expression levels were significantly decreased(<italic>P</italic><0.05,<italic>P</italic><0.01), while Smad7 protein and mRNA expression levels were significantly increased(<italic>P</italic><0.05,<italic>P</italic><0.01). Conclusion:Dahuang Zhechongwan ameliorates the alveolar inflammation, extracellular matrix (ECM) deposition, and fibrosis in mice with silicosis possibly by regulating the p38 MAPK/NF-<italic>κ</italic>B/TGF-<italic>β</italic><sub>1</sub> pathway.

9.
Article in Chinese | WPRIM | ID: wpr-906166

ABSTRACT

Dahuang Zhechongwan (DHZCW) is a classic prescription from the Jingui Yaolue(《金匮要略》) by ZHANG Zhong-jing,with the effects of tonifying deficiency, relaxing the middle, promoting regeneration, and resolving stasis. It has been widely used in the clinical treatment of various diseases with definite efficacy achieved. The research on multiple organ fibrosis has shown that DHZCW can slow down the development of organ fibrosis in the heart, liver, kidney, lung, etc., and good results in both clinical practice and experimental research have been obtained. The present study reviewed the previous investigations on the experimental mechanism of DHZCW in the treatment of multiple organ fibrosis and revealed that the pathogenesis was closely related despite different disease sites. From the perspective of traditional Chinese medicine (TCM),these diseases shared a common pathogenesis,which was manifested by deficiency. Long-term diseases led to the formation of "dried blood". From the perspective of modern medicine, the diseases all showed pathological changes in the deposition of extracellular matrix (ECM), and their occurrence and development were all based on certain effector cells [such as hepatic stellate cell (HSC) and pancreatic stellate cell (PSC)], with same cytokines [such as tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),IL-1β,and α-smooth muscle actin (α-SMA)] and some key pathways [transforming growth factor-β1 (TGF-β1)/Smad, phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt), and lipopolysaccharide (LPS) paracrine and autocrine mechanisms] involved. As a classic prescription for "deficiency-induced dry blood", DHZCW was effective in treating fibrosis, which was presumedly related to the inhibition of ECM deposition by intervening in the above-mentioned mechanisms, thereby delaying the disease progression. This study is expected to provide literature support to clarify the scientific connotation of DHZCW in the treatment of multiple organ fibrosis and lay a foundation for further experimental and clinical research.

10.
Article in Chinese | WPRIM | ID: wpr-905929

ABSTRACT

Objective:To explore the mechanism of gentiopicroside (GPS) in preventing acute liver injury induced by carbon tetrachloride (CCl<sub>4</sub>) in mice and its effect on the Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-<italic>κ</italic>B (NF-<italic>κ</italic>B) signaling pathway. Method:Sixty mice were randomly divided into a normal control group, a model group, a silymarin group (150 mg·kg<sup>-1</sup>), and high- (200 mg·kg<sup>-1</sup>), medium- (100 mg·kg<sup>-1</sup>), and low-dose (50 mg·kg<sup>-1</sup>) GPS groups, with 10 in each group. The mice in the groups with drug intervention were administered correspondingly by gavage at 10 mL·kg<sup>-1</sup>, and those in the normal control group and the model group receive an equal volume of distilled water, once per day. Ten days after administration, mice in the normal control group were subjected to the intraperitoneal injection of peanut oil (10 mL·kg<sup>-1</sup>) and those in other groups were injected with peanut oil (10 mL·kg<sup>-1</sup>) containing 0.12% CCl<sub>4 </sub>for the induction of acute liver injury model. After fasting for 16 hours, blood was collected from eyeballs and liver tissues were collected. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of liver tissues. The content or activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP), total superoxide dismutase (T-SOD), and <italic>γ</italic>-glutamyl transpeptidase (<italic>γ</italic>-GT) in the serum, malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in liver tissues were determined by biochemistry techniques. The levels of tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>), interleukin-1<italic>β</italic> (IL-1<italic>β</italic>), and interleukin-6 (IL-6) in liver tissues were measured by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the protein expression of TLR4, MyD88, and NF-<italic>κ</italic>B in liver tissues. The expression of phosphorylated NF-<italic>κ</italic>B (p-NF-<italic>κ</italic>B) was detected by immunohistochemistry. Result:Compared with the normal control group, the model group showed increased levels of ALT, AST, ALP, TBIL, <italic>γ</italic>-GT, and MDA (<italic>P</italic><0.01), and blunted activities of T-SOD and GSH-Px (<italic>P</italic><0.01). Compared with the model group, the high- and medium-dose GPS groups exhibited declining levels of ALT, AST, ALP, TBIL, <italic>γ</italic>-GT, and MDA (<italic>P</italic><0.05, <italic>P</italic><0.01) and potentiated T-SOD and GSH-Px activities (<italic>P</italic><0.05, <italic>P</italic><0.01). Compared with the normal control group, the model group displayed elevated levels of TNF-<italic>α</italic>, IL-1<italic>β</italic>, and IL-6 in liver tissues (<italic>P</italic><0.01) and increased protein expression of TLR4, MyD88, and p-NF-<italic>κ</italic>B (<italic>P</italic><0.01). Compared with the model group, the high- and medium-dose GPS groups showed decreased TNF-<italic>α</italic>, IL-1<italic>β</italic>, and IL-6 content in liver tissues (<italic>P</italic><0.05, <italic>P</italic><0.01) and dwindled TLR4, MyD88, and p-NF-<italic>κ</italic>B protein expression (<italic>P</italic><0.05, <italic>P</italic><0.01). Conclusion:GPS possesses a protective effect on mice with acute liver injury induced by CCl<sub>4</sub>, and its mechanism of action may be related to the regulation of TLR4/MyD88/NF-<italic>κ</italic>B signaling pathway and inhibition of oxidative stress.

11.
Article in Chinese | WPRIM | ID: wpr-905272

ABSTRACT

Brain-computer interface (BCI) technology can activate the plasticity of the nervous system and induce the reconnection of nervous system information pathways. Techniques for recording brain activity include invasive methods and non-invasive methods. Non-invasive methods are safer, more portable and cheaper, but are prone to signal pollution. For upper limbs rehabilitation in patients with severe chronic stroke, BCI of electroencephalogram and robotic arm is usually used, almost combined with other rehabilitation approaches, and seems to improve the effectiveness. It is necessary to improve the accuracy of BCI motion intention decoding, carry out hierarchical and customized treatment for patients, develop hybrid and portable BCI system.

12.
Article in Chinese | WPRIM | ID: wpr-905208

ABSTRACT

Objective:To observe the effects of a self-developed wearable intelligent upper limb rehabilitation robot system on upper limb motor function and activities of daily living of stroke patients. Methods:From August, 2018 to October, 2020, 61 stroke patients with hemiplegia from Beijing Bo'ai Hospital were randomly divided into control group (n = 30) and observation group (n = 31). The control group accepted routine rehabilitation training twice a day, while the observation group accepted routine rehabilitation training once a day and robot-assisted training once a day, for four weeks. All the patients were assessed with Fugl-Meyer Assessment-Upper Extremities (FMA-UE) and modified Barthel Index (MBI) before and after training, while the active range of motion (aROM) of affected shoulder, elbow and forearm was measured. Results:A case dropped out in the observation group. The scores of FMA-UE and MBI improved in both groups after training (|t| > 4.372, P < 0.001), and the improvement of MBI was more in the observation group than in the control group (t = 2.393, P < 0.05). The aROM of flexion/extension, internal/external rotation and abduction of shoulder, flexion of elbow, and supination/pronation of forearm increased in both groups (|t| > 3.050, P < 0.01), and the improvement of flexion and external rotation of shoulder was more in the observation group than in the control group (|t| > 2.160, P < 0.05). Conclusion:Robot-assisted training based on routine rehabilitation may promote the recovery of upper-limb function for stroke patients.

13.
Article in Chinese | WPRIM | ID: wpr-905187

ABSTRACT

Objective:To sort out the assistive device social security policy of Japan. Methods:The assistive devices social security policy for the older adults and the disabled in Japan was summarized in term of four dimensions: who, what, how to deliver and how to fund. Results:Japanese assistive device social security policy covered most of disabled groups according to the legal protections, delivering a variety of assistive devices products and services. Meanwhile, it has tried to avoid over expenditure through ways of lump-sum control, unit price and quantity control, users self-pay and strict evaluation system. For the service delivery system, the evaluators were strictly neutral, while the providers were in market. The funding came from user-payments, public finance and insurances, etc. Conclusion:Japan has established a mature social security policy system and service system for assistive devices, which can be used as a reference for China.

14.
Article in Chinese | WPRIM | ID: wpr-905171

ABSTRACT

Objective:To explore national standard of occupational skills for assistive technology consultants in China based on the study of relevant international standards. Methods:After analyzing the international and some other national job content, work requirements, professional education and vocational training, and vocational standards of assistive technology consultants, the national occupational skills standard for assistive technology consultants in China was established referring to the compiling rules of national occupational skills standard. Results:According to the compiling rules of national occupational skills standard, the national occupational skills standard for assistive technology consultants in China included career situation, basic requirements, job requirements, and weight table, etc. Conclusion:The vocational development, structural framework and content of professional capacity for assistive technology consultants are studied. The draft of national occupational skills standard for rehabilitation assistive technology consultants has been compiled.

15.
China Pharmacy ; (12): 2631-2634, 2021.
Article in Chinese | WPRIM | ID: wpr-904522

ABSTRACT

OBJECTIVE: To establish GC-MS method for the content determination of three toxic impurities in glycerophosphorylcholine raw materials ,such as epichlorohydrin ,glycidyl,3-chloro-1,2-propanediol. METHODS :Four batches of glycerophosphorylcholine raw materials were used as test samples. The determination was performed on ZB-WAXplus TM column, and the injector temperature was 200 ℃;the sample injection adopted splitless injection mode ,using helium (He)as carrier gas , in constant current mode ;the temperature program for the column was initially heating at 30 ℃ for 1 min,rising to 220 ℃ at a speed of 30 ℃/min then remaining 5 min. The ion source was electrospray ion source (EI),the ion source temperature was 200 ℃,and the ionization energy was 70 eV;transmission interface temperature was 250 ℃,and mass spectrum monitoring mode was selected ion (SIM). Detection ions were epichlorhydrin [mass charge ratio (m/z)49,57,62],glycidyl(m/z 31,43,44), 3-chloro-1,2-propanediol(m/z 44,61,79). The solvent delay time was 3 min. RESULTS :The linear range of epichlorhydrin , glycidyl and 3-chloro-1,2-propanediol were 29.86-746.48,172.91-922.18,21.18-211.85 ng/mL,respectively(all r>0.999 0). The detection limits were 19.91,115.27,10.59 ng/mL,respectively. The limits of quantitation were 29.86,172.91,21.18 ng/mL, respectively. RSDs of precision (n=6),reproducibility (n=6) and stability (placed at room temperature for 12 h,n=8) tests were all lower than 10%. The average recoveries were 93.88%,91.45%,91.86%,and RSDs were 5.10%,3.10%,2.49% (n=9),respectively. In the 4 batches of glycerophosphorylcholine raw materials ,three toxic impurities were all not detected. CONCLUSIONS:Established GC-MS method is simple ,efficient,accurate and repeatable ,and it can be used to determine the contents of three toxic impurities in glycerophosphorylcholine raw materials ,such as epichlorohydrin ,glycidyl,3-chloro-1, 2-propanediol.

16.
Article in Chinese | WPRIM | ID: wpr-888118

ABSTRACT

This study aimed to explore the characteristic role of Puerariae Lobatae Radix(PLR) in Gegen Decoction for the treatment of primary dysmenorrhea(PD). Estrogen(E_2) was combined with oxytocin to establish a mouse model of PD. The mice were randomly divided into a normal group, a model group, a Gegen Decoction group, a PLR-free Gegen Decoction group, a PLR group, and a positive drug group(ibuprofen). Writhing response times and writhing incubation of mice in each group were tested by behavio-ral assessment, and the serum levels of prostaglandin F_(2α)(PGF_(2α)), prostaglandin E_2(PGE_2), E_2, and progesterone(PROG) were detected by ELISA kits. Western blot method was adopted to detect cyclooxygenase-2(COX-2) and estrogen receptor alpha(ER_α) expression levels in uterine tissues. Doppler ultrasound was employed to detect changes in uterine artery blood flow in mice, including peak systolic blood flow velocity(maximum velocity), end-diastolic velocity(minimum velocity), peak systolic blood flow velocity/end-diastolic velocity(S/D), pulsatility index(PI), and resistive index(RI). Histopathological changes in the uterus were detected by HE staining. Based on the oxytocin-induced isolated uterine contraction model, the effects of Gegen Decoction, PLR-free Gegen Decoction, and PLR on the amplitude, frequency, and activity of isolated uterine contraction were compared to investigate the role of PLR in Gegen Decoction for the treatment of PD. The results showed that compared with the Gegen Decoction group, the PLR-free Gegen Decoction improved the indicators of PD except for E_2 content, ER_α expression, and uterine artery blood flow. PLR could significantly down-regulate the serum content of E_2 and the protein expression of uterine ER_α, and improve the uterine artery blood flow. The data suggested that PLR, as the sovereign drug of Gegen Decoction, might function in Gegen Decoction for the treatment of PD by mediating E_(2 )and improving the uterine artery blood flow.


Subject(s)
Animals , Drugs, Chinese Herbal , Dysmenorrhea/drug therapy , Humans , Mice , Plant Roots , Pueraria , Uterus
17.
Article in Chinese | WPRIM | ID: wpr-888071

ABSTRACT

This paper explores the statistical distribution characteristics of coating film thickness, so as to present a new method for determining coating endpoint based on 3σ criterion and logic regression. Firstly, the spectrum and thickness of 4 batch samples were collected. Secondly, the spectral range of normal products was obtained by 3σ criterion, with the spectral feature NI as the number of test spectrum in the above range. Then, the model based on 3σ criterion and logic regression was built according to the best condition in K-fold cross-validation and the determined threshold of qualified rate in the coating endpoint. Finally, the qualified rate of test set samples at different time points was calculated by the above model, and the above change trend and the threshold value were combined to determine the coating endpoint. The results of KS analysis showed the distribution of thickness of the qualified products followed the normal distribution(P=0.081>0.05). The accuracy of the coating endpoint determination was as high as 100% by the model based on 3σ criterion and logic regression when the determined threshold of qualified rate was 90%. Therefore, the 3σ criterion was feasible to the research of coating eligibility. This paper reveals certain random phenomena in the coating process, and the method features a high accuracy, quick analysis and a good interpretability, which provides a reference for online detection and qualify evaluation in future.


Subject(s)
Endpoint Determination , Logic , Research Design , Tablets
18.
Article in Chinese | WPRIM | ID: wpr-888050

ABSTRACT

To obtain the difference of the fungal and bacterial community diversity between wild Cordyceps sinensis, artificial C. sinensis and their habitat soil, Illmina Hiseq high-throughput sequencing technology was applied. The results show that Proteobacteria was the dominant bacterial phylum in C. sinensis, Actinobacteria was the dominant bacterial phylum in soil microhabitat, Ophiocordyceps sinensis was the predominant dominant fungus of C. sinensis. The α diversity analysis showed that the fungal diversity of stroma was lower than other parts, and the fungal diversity of wild C. sinensis was lower than that of artificial C. sinensis. The β diversity analysis showed that the fungal and bacterial community diversity of soil microhabitat samples was significantly different from that of C. sinensis. The fungal community diversity was less different between wild and artificial C. sinensis, especially in sclerotia. LEfSe analysis showed a lot of species diversity between wild and artificial C. sinensis. Those different species between wild C. sinensis, artificial C. sinensis and their habitat soil provide ideas for further research on breed and components of C. sinensis.


Subject(s)
Cordyceps/genetics , High-Throughput Nucleotide Sequencing , Microbiota/genetics , Soil , Soil Microbiology
19.
Article in Chinese | WPRIM | ID: wpr-882441

ABSTRACT

Enhanced recovery after surgery (ERAS)is a new concept of accelerating the recovery of patients through a series of multi-mode strategies based on evidence-based medicine date in perioperative period. The perioperative fluid therapy is an important part of ERAS, including three phases of preoperative、intraoperative and postoperative. Its aim is to maintain the circulation volume to ensure circulation stability and effective perfusion of tissues, to avoid tissue ischemia and hypoxia, and to reduce surgical stress, maintain internal environment stability, reduce postoperative respiratory and circulatory complications, thus accelerating recovery. Fluid therapy has been controversial, Goal-directed Fluid Therapy is a recognized method.This article reviews the latest advances in preoperative, intraoperative and postoperative fluid therapy on the guidance of ERAS and its influence on postoperative outcomes.

20.
Chinese Journal of School Health ; (12): 872-875, 2021.
Article in Chinese | WPRIM | ID: wpr-881366

ABSTRACT

Objective@#To understand the incidence of school bullying and its influencing factors among adolescents in low-income and middle-income countries, and to explore the association between school bullying and mental health of adolescent students, so as to provide reference for prevention and control of school bullying and mental health intervention.@*Methods@#Data was obtained from the 2009-2015 Global School Student Health Survey from 19 low-income and middle-income countries (n=22 963). Binary Logistic regression was used to analyze the influencing factors of school bullying, and multiple linear regression was used to analyze the relationship between school bullying and mental health.@*Results@#The average score of students mental health was(5.75±2.09), and approximately 35.1% of adolescent students reported suffering from school bullying. The rates of school bullying among students in low-income and middle-income countries were 39.4% amd 34.3%, respectively. Students with lower grades, overweight, poor family economic status, low family learning and psychological support, poor perceived family relationship, more truancy, and poor relationship with classmates were more likely to suffer from school bullying(P<0.05). Exposure to school bullying was positively associated with adverse mental health outcomes for women(B=1.27, P<0.01).@*Conclusion@#Not only were school bullying more common in low-income countries, but also school bullying had a greater negative impact on the mental health of girls. We need to pay more attention to school bullying among adolescent students, especially in low-income countries and girls, with cost-effective interventions to reduce or mitigate the consequences of bullying.

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