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Article in Chinese | WPRIM | ID: wpr-879038


Myocardial ischemia-reperfusion injury(MIRI) is an urgent problem in clinical treatment. As cardiomyocytes are terminal cells, MIRI-induced cardiomyocyte death will irreversibly damage the structure and function of the heart. In previous studies, apoptosis was considered to be the only way to regulate cell death, while necrosis could not be regulated. However, current studies have shown that cell necrosis could also be regulated, which was collectively called programmed cell death(PCD). Regulated cell death is actively mediated through molecular pathways, so there is the possibility of inhibiting this signaling to reduce MIRI. At present, PCD mainly includes apoptosis, autophagy, necrosis, pyroptosis and ferroptosis. As a unique treature in China, traditional Chinese medicine has the advantages of multiple pathways, multiple targets, low toxicity, less side effects and low economic costs. With the in-depth study of the efficacy of traditional Chinese medicine against MIRI, it has been confirmed that traditional Chinese medicine could regulate PCD to reduce MIRI. Therefore, this paper focuses on the relationship between PCD and MIRI, and new studies on intervention with relevant traditional Chinese medicine, with the aim to provide new MIRI prevention and treatment methods from the perspective of "intervention of PCD".

Apoptosis , China , Humans , Medicine, Chinese Traditional , Myocardial Reperfusion Injury/genetics , Myocytes, Cardiac
Article in Chinese | WPRIM | ID: wpr-872669


Myocardial ischemia-reperfusion injury (MIRI) was originated from the ischemic injury to myocardial cells due to some factors, and the injury will be aggravated after the blood supply recovery. MIRI will cause reperfusion arrhythmia, myocardial stagnation, microcirculation disorders or blood loss reflow, and become a key issue to be solved in the clinical treatment. Therefore, it is of important clinical significance to explore how to improve MIRI. The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway has been regarded to be an important cascade signaling pathway to prevent MIRI, which is cross-talked in such mechanisms as oxidative stress, calcium overload, autophagy, inflammation, endoplasmic reticulum stress and mitochondrial dysfunction. This pathway is at the core site and closely related to the severity of MIRI. Meanwhile, traditional Chinese medicine(TCM) has its unique advantages in the prevention and treatment of MIRI, including multiple targets, multiple pathways, fewer toxic and side effect, as well as TCM characteristics of syndrome differentiation treatment and overall concept. A great number of studies have confirmed that this pathway is a common mechanism of action for most TCM compounds, TCM monomers and extracts. In this paper, we clarify the effect of PI3K/Akt signaling pathway crosstalk on the myocardial ischemia-reperfusion injury, with the focus on the relationship between the key targets of PI3K/Akt signaling pathway and MIRI. It will give great insights to the intricate relationship between them and promote the future development of new drugs targeting the PI3K/Akt signaling pathway. Finally, we also summarized the existing findings of TCM on PI3K/Akt pathway, which provides a reference for the treatment and drug development of MIRI.

Article in Chinese | WPRIM | ID: wpr-256571


<p><b>OBJECTIVE</b>To compare the sensitivity and reproducibility of Allglo and TaqMan probe in the detection of simian immunodeficiency virus (SIV) using fluorescence quantitative RT-PCR (QPCR).</p><p><b>METHODS</b>The reference sample of SIV was diluted to 6 gradient concentrations; at each concentration 12 samples were tested to analyze the variations within batches, and each sample was tested for 12 times for analysis of variations between batches by QRT-PCR using TaqMan probe and Allglo probe. The results of QPCR using the two probes were analyzed with ABI7300 PCR system software.</p><p><b>RESULTS</b>In QPCR using TaqMan and Allglo probe, the lower limit of sensitivity for SIV detection was both 50 copies/mL. Assessment of the reproducibility of the tests showed that the maximum and minimum coefficients of variation between batches were 0.63% and 0.33% with Allglo probe, respectively, as compared with 1.33% and 0.2% with TaqMan probe. The maximum and minimum coefficients of inter-batch variation was 1.77% and 0.95% with Allglo probe, respectively, as compared with 1.86% and 1.03% with TaqMan probe.</p><p><b>CONCLUSION</b>Allglo probe shows a better performance then TaqMan probe in detection of SIV QPCR.</p>