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1.
Article in Chinese | WPRIM | ID: wpr-888059

ABSTRACT

Paris polyphylla var. chinensis(PPC) is used as one of the origin plants of Paridis Rhizoma described in the Chinese Pharmacopoeia(2020 edition). Its resources shortage makes the planting scale gradually expand, and plenty of aerial parts are abandoned because of not being effectively used. On the basis of previous research, this study separated steroidal saponins to further clarify the chemical composition of the aerial parts of PPC. As a result, three pairs of 25R or 25S epimers of furostanol saponins were obtained by various column chromatography techniques. Their structures were identified as neosolanigroside Y6(1), solanigroside Y6(2), neoprotogracillin(3), protogracillin(4), neoprotodioscin(5) and protodioscin(6) by spectral data combining with chemical transformation. Compound 1 is a new compound, and compounds 2, 3 and 5 are isolated from Paris plants for the first time. Compounds 4 and 6 are isolated from this plant for the first time. Previously, only several spirostanol glycosides with 25S configuration were isolated from Paris plants. Guided by mass spectrometry, the present study isolated the furostanol saponins with 25S configuration from this genus for the first time, which further enriches the chemical information of Paris genus and provides a reference for the isolation of similar compounds.


Subject(s)
Liliaceae , Melanthiaceae , Plant Extracts , Rhizome , Saponins
2.
Article in Chinese | WPRIM | ID: wpr-888028

ABSTRACT

Paridis Rhizoma(PR) is prepared from the dried rhizome of Paris polyphylla var. yunnanensis(PPY) or P. polyphylla var. chinensis(PPC) in Liliaceae family. The rapid development of PPY or PPC planting industry resulted from resource shortage has caused the waste of a large number of non-medicinal resources. To clarify the chemical compositions in rhizomes, fibrous roots, stems, leaves, seeds and pericarps of PPC, and explore the comprehensive application value and development prospect of these parts, the qualitative and quantitative analyses on the different parts of PPC were carried out by ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS) and high performance liquid chromatography(HPLC). A total of 136 compounds were identified, including 112 steroidal saponins, 6 flavonoids, 11 nitrogen-containing compounds and 7 phytosterols. Rhizomes, fibrous roots, and seeds mainly contained protopennogenyl glycosides and pennogenyl glycosides; leaves and stems mainly contained protodiosgenyl glycosides and diosgenyl glycosides; pericarps mainly contained pennogenyl glycosides, followed by diosgenyl glycosides. The total level of four saponins was the highest in fibrous roots and rhizomes, followed by those in the pericarps and arillate seeds, and the lowest in the stems and exarillate seeds. This study can provide data support for the comprehensive development and rational application of non-medicinal parts of PPC.


Subject(s)
Chromatography, High Pressure Liquid , Liliaceae , Melanthiaceae , Rhizome , Saponins , Tandem Mass Spectrometry
3.
Article in Chinese | WPRIM | ID: wpr-872705

ABSTRACT

Objective:To investigate the effect of Ru′ai Shuhou prescription (RSR) drug-containing serum on the proliferation and invasion ability of breast cancer cells MDA-MB-453 based on the biological axis of stromal cell-derived factor-1(SDF-1)/chemokine receptor 4 (CXCR4). Method:A model of MDA-MB-453 cells with SDF-1-induced high expression of CXCR4 was established, and the rat drug-serum containing RSR and blank rat serum were prepared respectively. The cells were divided into fetal bovine serum control group (Blank), blank rat serum group, SDF-1+blank rat serum group, SDF-1+RSR group, AMD3100+ SDF-1+blank rat serum group, and AMD3100+ SDF-1+RSR group. After intervention for 48 h, cell proliferation was detected by cell counting kit-8 (CCK-8) assay, cell invasion ability was detected by transwell assay, and mRNA and protein expressions of CXCR4, matrix metalloproteinase-2 (MMP-2) and MMP-9 were detected by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) and Western blot, respectively. Result:As compared with the blank serum group, the proliferation of MDA-MB-453 cells was promoted and expression of CXCR4 mRNA was increased significantly when SDF-1 was 100 μg·L-1 (P<0.05). As compared with SDF-1+blank rat serum group, RSR inhibited the proliferation and invasion of MDA-MB-453 cells induced by SDF-1, and at the same time, down-regulated the mRNA and protein expressions of CXCR4, MMP-2 and MMP-9 (P<0.05). After pre-treatment with AMD3100 for 24 h, the inhibitory effect of RSR to cell proliferation was significantly increased (P<0.05), and meanwhile, the decreases in mRNA and protein expression of CXCR4, MMP-2 and MMP-9 were more obvious, with statistically significant differences (P<0.05). Conclusion:Through SDF-1/CXCR4 biological axis, RSR could down-regulate the expression of MMP-2 and MMP-9, reduce the degradation of extracellular matrix (ECM), and then inhibit the metastasis of MDA-MB-453 cells. In addition, it has a synergistic effect with CXCR4 inhibitor AMD3100.

4.
Article in Chinese | WPRIM | ID: wpr-801905

ABSTRACT

Objective:To study the appearance description,TLC examination and content determination was carried out, in order to improve the quality standard of processed slices of Paridis Rhizoma in the 2020 edition of Chinese Pharmacopoeia. Method:Based on the literature review and observation on the samples,the appearance description was described. TLC examination was used for the qualitative analysis. HPLC was used for the determination of polyphyllin Ⅰ,Ⅱ,Ⅵ and Ⅶ in the commercial and processed samples. UPLC was employed for the determination of 10 steroidal saponins,namely pseudoprotodioscin,polyphyllin Ⅶ,17-hydroxygracillin,polyphyllin H,polyphyllin Ⅵ,polyphyllin Ⅱ,dioscin,gracilin,polyphyllin Ⅰ and polyphyllin Ⅴ. Result:For the appearance description,color and luster,texture,odor and taste as well as the diameter of 1.0-4.5 cm were recorded. polyphyllin Ⅵ was not detected in the thin layer chromatograms of most of the tested samples derived from high-quality species but obviously detected in those of Trillium Rhizoma. Five of 13 commercial samples met the requirements that the total amounts of polyphyllin Ⅶ,Ⅵ,Ⅱ,and Ⅰ should be no less than 0.6%according to the current Chinese Pharmacopoeia. Because softening and drying had the obvious influence on the contents of steroidal saponins in the samples,soaking and sun-drying were preferred. Conclusion:Appearance description should be supplemented. Polyphyllin Ⅵ is not considered as one of quality markers for the TLC identification and HPLC determination of Paridis Rhizoma. Polyphyllin H was considered as a new marker for the quality control. It is recommended that the total amounts of polyphyllin Ⅶ,H,Ⅱ,and Ⅰ should be no less than 1.0%.

5.
National Journal of Andrology ; (12): 1082-1085, 2014.
Article in Chinese | WPRIM | ID: wpr-319563

ABSTRACT

<p><b>OBJECTIVE</b>To explore the efficacy and safety of vitamin D (VD) in the treatment of idiopathic oligoasthenozoospermia.</p><p><b>METHODS</b>This study included 86 infertile men with idiopathic oligoasthenozoospermia, who were randomized to a VD and a control group of equal number, the former given oral VD 200 IU/d and calcium 600 mg/d,qd, while the latter administered oral vitamin E 100 mg and vitamin C 100 mg, tid. After 3 months of medication, we compared the semen parameters, adverse reactions, and pregnancy rate between the two groups.</p><p><b>RESULTS</b>After medication, the count of progressively motile sperm per ejaculate was increased from (9.82 ± 3.72) x 10(6) to (21.47 ± 6.52) x 10(6) ( P < 0.05) and the proportion of progressively motile sperm from (18.41 ± 9.82)% to (28.27 ± 4.47)% (P < 0.05) in the VD group. In comparison, the count of progressively motile sperm per ejaculate was elevated from (9.51 ± 6.31) x 10(6) to (12.36 ± 4.43) x 10(6) (P > 0.05) and the proportion of progressively motile sperm from (17.79 ± 5.25)% to (21.35 ± 2.41)% (P > 0.05) in the control group. Pregnancy was achieved in 7 cases (16.3%) in the VD group, but only lease (2.3%) in the control (P < 0.05). No adverse reactions were observed in either of the groups.</p><p><b>CONCLUSION</b>Vitamin D, as a safe option for the treatment of idiopathic oligoasthenozoospermia, can effectively improve the semen quality, especially the progressive sperm motility of the patient.</p>


Subject(s)
Administration, Oral , Adult , Asthenozoospermia , Drug Therapy , Female , Humans , Male , Pregnancy , Pregnancy Rate , Semen , Physiology , Semen Analysis , Sperm Motility , Vitamin D , Therapeutic Uses , Vitamin E , Therapeutic Uses , Vitamins , Therapeutic Uses
6.
Article in Chinese | WPRIM | ID: wpr-283370

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of Xianlong granules (XLG) on immunological function in the rat of adjuvant arthritis (AA).</p><p><b>METHOD</b>Rats were randomly divided into normal group, AA model group, prednisone group and low, middle and high dose XLG groups, 10 rats in each group. All rats were treated by intragastric administration from the 18 days after arthritis was induced by the complete Freud's adjuvant and the effect of XLG on toes swelling was observed. On the 30th days after modeling, proliferation of the splenic and thymic lymphocytes, and IgG secreted by splenocytes were detected respectively by MTT assay and ELISA.</p><p><b>RESULT</b>Compared with the model group, both the high and middle dose XLG groups had significant therapeutic effects on toes dwelling in the rat of AA (P < 0.05 or P < 0.01); The low, middle and high dose XLG groups strengthened the PHAM-inhibited proliferation of splenic lymphocytes (P < 0.05), and inhibited the PHAM-augmented proliferation of thymic lymphocytes (P < 0.05); XLG did not significantly effect on IgG level secreted by splenocytes in rats of AA.</p><p><b>CONCLUSION</b>XLG can cure toes swelling in rats of AA, which is related with regulation of the abnormal immunlological function.</p>


Subject(s)
Animals , Arthritis, Experimental , Allergy and Immunology , Pathology , Cell Proliferation , Colubridae , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Edema , Allergy and Immunology , Pathology , Female , Immunoglobulin G , Metabolism , Lymphocytes , Pathology , Bodily Secretions , Male , Materia Medica , Pharmacology , Medicine, Chinese Traditional , Plants, Medicinal , Chemistry , Random Allocation , Rats , Rats, Wistar , Spleen , Pathology , Bodily Secretions , Thymus Gland , Pathology , Toes , Pathology
7.
Article in Chinese | WPRIM | ID: wpr-638651

ABSTRACT

0.05).2.Neonates umbilical serum leptin concentration was positively correlated with body mass index(r=0.520 P

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