ABSTRACT
Objective To explore the effect and mechanism of penehyclidine hydrochloride (PHCD) on vascular endothelial injury in septic rats. Methods Fifty male SD rats were randomly divided into control group, lipopolysaccharide (LPS) induced sepsis group (model group), low dose PHCD (0.3 mg/kg) group, medium dose PHCD (1.0 mg/kg) group and high dose PHCD (3.0 mg/kg) groups, ten mice for each group. Normal saline was injected into the tail vein of the control group, and 10 mg/kg lipopolysaccharide (LPS) was injected into the tail vein of the rats in other groups to prepare the sepsis rat models. After the models were successfully established, low, medium and high doses (0.3, 1.0, 3.0 mg/kg) of PHCD solution were injected into the tail vein of the rats of corresponding groups. Wet/dry mass ratio (W/D) of lung tissue of rats in each group was measured, and ELISA was used to assay interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 content and rat plasma angiopoietin 2 (Ang2) content in bronchoalveolar lavage fluid (BALF). HE staining was used to observe the pathological changes of lung tissues. Immunohistochemical staining was used to observe the expression of Ang2 in the right lung tissues. Western blot analysis was performed to detect Ang2 and vascular endothelial cadherin (VE-cadherin) protein in lung tissues. Results Compared with the control group, the W/D ratio of the lung tissues of rats in the model group and the contents of IL-1β, IL-6 and TNF-α in BALF were significantly increased; the lung tissues showed obvious pathological damage, with up-regulation of Ang2 expression and down-regulation of VE-Cadherin expression. Compared with the model group, the W/D ratio of the lung tissues of rats in three PHCD treatment groups and the contents of IL-1β, IL-6 and TNF-α in BALF were significantly reduced; the pathological damage of lung tissue was significantly reduced, with down-regulation of Ang2 expression and up-regulation of VE-cadherin expression. Conclusion PHCD can reduce LPS-induced lung inflammation in rats with sepsis by regulating the Ang2/VE-Cadherin pathway, thereby improving vascular endothelial injury.
Subject(s)
Rats , Mice , Animals , Male , Lipopolysaccharides/metabolism , Tumor Necrosis Factor-alpha/metabolism , Angiopoietin-2/pharmacology , Interleukin-6/metabolism , Rats, Sprague-Dawley , Lung , Acute Lung Injury/metabolism , Sepsis/metabolismABSTRACT
Objective To survey the current status and training requirement of rural doctors in Hebei province.Methods A questionnaire survey was conducted among1 200 rural doctors selected from 11 cities of Hebei province by stratified sampling from October to December 2017.The self-designed questionnaire included general condition,job satisfaction,impact of medical reform policies,training needs of rural doctors.Results A total of 1 200 questionnaires were distributed and 1 170 valid ones were retrieved with a recovery rate of 97.5%.Among the 1 170 responders,there were 726 males(62.1%) and 444 females (37.9%);748 (63.9%) were aged more than 40 years;612 (52.3%) hold secondary school degree;826 (70.6%) had been working in the village for more than 10 years,and the overall job satisfaction rate was 30.8% (360/1 170).Among all responders,73.8% (864/1 170) worked in the standardized village clinics,and 72.0% (842/1 170) were contracted with local residents as family doctors;52.8% (618/1 170) responders believed that the reform policy improved the health care for villagers.In all responders,98% (1 147/1 170)thought they needed training,93.6% (1 095/1 170) had received training.The training contents they most needed werethe management of common diseases (904,77.3%),followed by medical humanities(650,55.6%)and first aid knowledge (492,42.1%).The influencing factors for training were lack of time,no energy (656,54.3%),lack of appropriate training forms (510,43.6%),economic reasons (476,40.3%) and so on.Conclusion The working status of rural doctors need to befurther improvedand the training need to be strengthened to increase their job satisfaction.
ABSTRACT
Objective To study the effect of survivin on proliferation and invasion of glioma cells treated by bevacizumab (Bev). Methods The human spongioblastoma cell line U87 was routinely cultured in vitro; the growth rates of U87 cells after 0, 2, 4, 6, 8 and 10 mg/mL Bev treatment for 2, 4 and 6 d were determined by MTT assay. The specific shRNA vectors (pRNAT, pRNAT-survivin shRNA, pRNAT-NS si) were transfected into U87 cells; U87, U87/sur(-), U87/pCtrl and U87/NS si cells were cultured for one d, and then, they were divided into 6 groups: U87 cells without Bev treatment, U87/sur (-) cells without Bev treatment, U87 cells with Bev treatment, U87/sur (-) cells with Bev treatment, U87/pCtrl cells with Bev treatment and U87/NS si cells with Bev treatment; 6 mg/mL Bev was given to each Bev treatment group. The cell invasion capacity was determined by Traswell assay; the cell movement and migratory capacities were detected by wound-healing assay, and the cell proliferation was determined by MTT assay. Results The cells treated by Bev at concentrations of 0, 2, 4, or 6 mg/mL exhibited similar viability (P>0.05), while the cells treated by Bev at concentrations of 8 or 10 mg/mL showed significantly decreased viability as compared with cells treated by Bev at concentration of 0 mg/mL (P<0.05). As compared with U87 cells with Bev treatment, U87/sur(-) cells with Bev treatment had significantly decreased viability (P<0.05); U87/sur (-) cells with Bev treatment had significantly decreased viability as compared with U87/sur(-) cells without Bev treatment (P<0.05); as compared with that in the U87 cells without Bevb treatment, the migration distance in the U87 cells with Bev treatment was significantly longer (P<0.05). As compared with that in the U87 cells without Bev treatment, the migration distance in the U87/sur (-) cells with Bev treatment was significantly shorter (P<0.05); as compared with that in the U87 cells without Bev treatment, the number of cells invading into the lower chamber in the U87 cells with Bev treatment was significantly larger (P<0.05); as compared with that in the U87 cells without Bev treatment, the the number of cells invading into the lower chamber in the U87/sur(-) cells with Bev treatment was significantly smaller (P<0.05). Conclusion Down-regulation of survivin could suppress glioma cells invasion induced by Bev treatment, and synergistic effect is observed between down-regulation of survivin and Bev treatment in suppressing the viability of glioma cells.
ABSTRACT
Objective@#To provide biomechnical basis for orthodontics of centronuclear myopathy (CNM) patients, we studied the oral and maxillofacial clinical features and MRI image manifestations to explore application of MRI to objective evaluation the affected facial muscles.@*Methods@#The study consisted of 8 patients who were diagnosed as CNM (CNM group) and 20 healthy volunteers (control group). Their medical information were gathered and then we examined the ptosis situation and the facial index calculation of them. To measure the maximal hight of palate and the width of palate, patients and volunteers were made impressions. We also checked their maximum bite force with occlusion pressure tester. And they took lateral cephalometric radiographs to measure mandibular plane-Frankfort horizontal plane angle (MP-FH). At last, they were taken oral and maxillofacial region MRI to observe the affected situation of masseter muscle, medial pterygoid muscle and lateral pterygoid muscle.@*Results@#Six patients were ptosis; 6 patients had inverse V-shaped mouth; 3 patients were difficulty in swallowing; 4 patients were anterior open bites; 4 patients were mouth breathing; 7 patients liked to eat soft foods. Morphological facial index ([91.3±0.5]%), MP-FH (34.9°±2.0°) of CNM group were greater than the control group, male maximal hight of palate ([19.0±0.2] mm), female maximal hight of palate ([18.0±0.6] mm) of CNM group were greater than the control group (P<0.05). Male width of palate ([34.5±0.8] mm), female width of palate ([33.4±1.0] mm), male maximum bite force ([464.3±78.2] N), female maximum bite force ([320.7±13.8] N), maximal opening of mouth ([3.4±0.3] cm) of CNM group were less than the control group (P<0.05). And these had significant difference compared with the control group (P<0.05). In MRI examination, there were 7 patients' masseter muscles, 4 patients' medial pterygoid muscles and 6 patients' lateral pterygoid muscles to atrophy asymmetrically. These three pieces of muscular fatty infiltration were inordinately, focused on Grade 0 to 4 and the both sides were similar.@*Conclusions@#CNM patients with long and thin face, high palatine arches and low bite force together were the biomechanical basis of the maxillofacial deformities. MRI can clearly show the affected masseter muscle, medial pterygoid muscle, lateral pterygoid muscle, and can serve as an objective examination method for the evaluation of facial muscles. It can be worth of clinical popularization and application.
ABSTRACT
Phosphatase of regenerating liver-3 (PRL-3) is one of member in the protein tyrosine phosphatase (PTP) family.Since high expression of PRL-3 was first discovered in liver metastasis patients with colon cancer,many researchers found that PRL-3 also high expressed in multiple organs like cancer from breast,prostate,liver,stomach,lung,kidney and so on.This paper focused on correlation of PRL-3 with the occurrence and metastasis of tumors.
ABSTRACT
Objective To explore the effect of meta-mood ability training for improving job satisfaction of nurses.Methods A total of 120 nurses were randomized into the observation group and the control group (60 cases in each group).The observation group received meta-mood ability training,while the control group was given routine training.The meta-mood ability and job satisfaction degree were investigated.Results After the intervention,job satisfaction degree and the meta-mood ability level of the observation group nurses were improved than that of the control group.Conclusions The meta-mood ability training is effective in improvement of job satisfaction degree of nurses,as well as increase clinical service quality of nurses.
ABSTRACT
Objective To evaluate the application of mobile ultrasonoscope in earthquake rescue. Methods According to the characteristic of environment and traumatic conditions in earthquake area, the mobile ultrasonoscope was used to diagnose for clinicians, and the advantages and disadvantages were evaluated. Results The mobile ultrasonoscope played an important role in routine diagnosis, such as organic injury and calculus, superficial organellae injury, soft tissue injury, injury of limbs and blood vessels, gynaecology and obstetrics and so on. As far as certain conditions were conferred in which the mobile ultrasonoscope was seldom used to diagnose in common practice, it played a dramatic role too in bone fracture, joint damage, craniocerebral injury and foreign objects. Conclusion In routine diagnosis or certain conditions, the mobile ultrasonoscope is very practical, especially in earthquake rescue.
ABSTRACT
1.The nutrient content of yeast (Condida Y-17) grown on n-alkane studied in this experiment was comparable to that of the same type of yeast produced in other countries. Its content of lead, arsenic, mercury and benzo (a) pyrene were within the limit proposed by the Protein-Calorie Advisory Group of the United Nations System. The Content of its residual n-alkanes and total aromatic hydrocarbons were within or a little over that limit. It is a good supplementary protein feed.2.The corrected PER values were, yeast grown on n-alkane 1.18; deli pid and denucleic acid yeast grown on n-alkane 1.16; brewer's yeast 1.52; full fat soybean meal 1.81. The PER of yeast grown on n-alkane was significantly lower than those of other samples. When 0.3% DL-methionine was added to the diet, the corrected PER values increased: n-alkane grown yeast 2.32; delipid and denucleic acid n-alkane grown yeast 2.49; brewer's yeast 2.35; full fat soybean meal 2.28. The corrected PER value of casein used for correction was 2.43, it was standardized with ANRC reference protein (PER = 2.50).3.Yeast grown on n-alkane with and without removal of lipids and nucleic acid was fed to groups of rats at a dietary level of 25% and 35% respectively for 1 year. A stock diet group and a brewer's yeast diet group were used as control. In the first 3 months the weight gain and feed consumption of the rats fed n-alkanc grown yeast diet were lower than those of the two control groups. The male rats were more sensitive to the change of diet. In the later 9 months the difference of weight gain among different groups were not significant. The concentration of haemoglobin, red cell counts, white cell counts, GPTase activity, serum cholesterol levels and serum urea levels determined at the beginning and the end of the experiment were all in the normal ranges. The concentrations of n-alkanes and odd-chain fatty acids in the adipose tissue of the n-alkane grown yeast group were higher than those of the two control groups, and the difference was highly significant. The concentrations of total aromatic hydrocarbons and benzo(a) pyrene in the muscle of different groups of rats were 0.4-0.6 ppm and 0.4 ppb respectively. No detrimental effect was observed in pathological examination.4.When 5% fish meal or 3.5% casein was added to the 25% n-alkane grown yeast diet, the weight gain and feed consumption of weanling rats were improved significantly, though it was still inferior to those fed with stock diet.5.Strongly growth depressive effect was noted as the level of n-alkane grown yeast was increased to 20% in the diet. This depressive effect was neither due to the toxicity of the residual n-alkanes and aromatic hydrocarbons, nor due to the deficiencies of potassium and selenium in the diet. It might be the result of nutrient or nutrients imbalance of the diet. The appropriate amount of yeast grown on n-alkane used in mixed feed should not be more than 15%.