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1.
Article in Chinese | WPRIM | ID: wpr-934584

ABSTRACT

Objective: To observe the effect of moxibustion on the colonic mucosal barrier of rats with ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). Methods: Forty male Sprague-Dawley rats were randomly divided into a normal group and a modeling group, with 20 rats in each group. Rats in the modeling group were subjected to preparing experimental UC models by drinking 4% DSS for seven consecutive days. Two modeled rats and two normal rats were randomly selected for model identification. After the success of UC model was confirmed, the remaining 18 modeled rats were randomly divided into three groups, a model group, a model + herbal cake-partitioned moxibustion group, and a model + mild moxibustion group, with six rats in each group; the remaining normal rats were randomly divided into three groups, a normal group, a normal + herbal cake-partitioned moxibustion group, and a normal + mild moxibustion group, with six rats in each group. After 7 d of intervention with the herbal cake-partitioned moxibustion or the mild moxibustion, hematoxylin-eosin (HE) staining technique was used to observe the pathological changes of colon tissue under a light microscope; Western blotting and/or immunohistochemical techniques were used to detect the protein expression levels of Occludin, Claudin, junction adhesion molecular 1 (JAM1), mucin 2 (MUC2), and transforming growth factor beta1 (TGF-β1) in rat colon tissue. Results: Compared with the normal group, the colon tissue was severely damaged, the pathological score was significantly increased, and the protein expression levels of Occludin, Claudin, JAM1, MUC2, and TGF-β1 were significantly decreased in the model group (P<0.01); while there were no significant differences in the colonic histopathological score, protein expression levels of Occludin, Claudin, JAM1, MUC2, and TGF-β1 in the normal + herbal cake-partitioned moxibustion group and the normal + mild moxibustion group (P>0.05). Compared with the model group, the model + herbal cake-partitioned moxibustion group and the model + mild moxibustion group showed repaired colon tissue, ulcer healing, significantly reduced pathological score, and significantly increased protein expression levels of JAM1, MUC2, and TGF-β1 (P<0.05); the Occludin protein expression level in the colon tissue of the model + mild moxibustion group was increased (P<0.01). Conclusion: Neither herbal cake-partitioned moxibustion nor mild moxibustion influences the colonic histopathology and intestinal mucosal barrier-related protein expression in the normal rats; both herbal cake-partitioned moxibustion and mild moxibustion can up-regulate the protein expression levels of JAM1, MUC2, and TGF-β1 in the colon tissue of UC rats. Mild moxibustion can up-regulate Occludin protein expression. This may be a mechanism of moxibustion in reducing colonic mucosa inflammation in UC.

2.
Article in Chinese | WPRIM | ID: wpr-928405

ABSTRACT

OBJECTIVE@#To carry out genetic testing for a patient with 45,X/46,XY mosaicism and autism spectrum disorder (ASD).@*METHODS@#Peripheral blood samples of the patient and his parents were collected for the extraction of genomic DNA. Trio-based whole exome sequencing and Sanger sequencing were carried out thereafter.@*RESULTS@#The proband and his father were found to harbor a heterozygous c.4781G>A (p.Arg1594Gln) variant of the CACNA1I gene. In addition, the proband was also found to harbor a de novo c.268C>T (p.Arg90Trp) missense variant of the MTRR gene. Based on guidelines of the American College of Medical Genetics and Genomics (ACMG), the c.4781G>A (p.Arg1594Gln) variant of the CACNA1I gene was predicted to be pathogenic (PVS1, PM1, PM2, PP3), while the c.268C>T (p.Arg90Trp) variant of the MTRR gene was predicted to be of uncertain significance.@*CONCLUSION@#Variants of the CACNA1I and MTRR genes, together with the chromosomal mosaicism, may have predisposed to the susceptibility to the ASD in this patient.


Subject(s)
Autism Spectrum Disorder/genetics , Genomics , Heterozygote , Humans , Mosaicism , Whole Exome Sequencing
3.
Article in Chinese | WPRIM | ID: wpr-888360

ABSTRACT

OBJECTIVE@#To analyze the pathogenic variants of the KIF1A gene and its corresponding protein structure in an autism spectrum disorder (ASD) family trio carrying harmful missense variants in the KIF1A gene.@*METHODS@#The peripheral blood DNA of the patient and his parents was extracted and sequenced using whole exome sequencing (WES) technology and verified by Sanger sequencing. Bioinformatics software SIFT, PolyPhen-2, Mutation Taster, and CADD software were used to analyze the harmfulness and conservation of variants. The Human Brain Transcriptome (HBT) database was used to analyze the expression of the KIF1A gene in the brain. PredictProtein and SWISS-MODEL were further used to predict the secondary structure and tertiary structure of KIF1A wild-type protein and variant protein. PyMOL V2.4 was utilized to investigate the change of hydrogen bond connection after protein variant.@*RESULTS@#The WES sequencing revealed a missense variant c.664A>C (p.Asn222His) in the child's KIF1A gene, and this variant was a de novo variant. The harmfulness prediction results suggest that this variant is harmful. By analyzing expression level of KIF1A gene in the brain. It is found that KIF1A gene widely expressed in various brain regions during embryonic development. By analyzing the variant protein structure, the missense variant of KIF1A will cause many changes in the secondary structure of protein, such as alpha-helix, beta-strand, and protein binding domain. The connection of hydrogen bond and spatial structure will also change, thereby changing the original biological function.@*CONCLUSION@#The KIF1A gene may be a risk gene for ASD.


Subject(s)
Autism Spectrum Disorder/genetics , Child , Female , Humans , Kinesins/genetics , Mutation , Mutation, Missense , Pregnancy , Protein Domains , Whole Exome Sequencing
4.
Article in Chinese | WPRIM | ID: wpr-882236

ABSTRACT

Objective:To investigate the efficiency and pharmacoeconomics of pegylated recombinant human granulocyte colony-stimulating factor (PEG-rhG-CSF) for mobilization of peripheral blood stem cells (PBSCM) in patients with multiple myeloma (MM).Methods:The data of 91 patients with newly treated MM who were hospitalized in the First Hospital of Jilin University and Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College from January 2015 to October 2017 were retrospectively analyzed. According to the patient's wishes, a high-dose chemotherapy combined with subcutaneous injection of PEG-rhG-CSF or recombinant human granulocyte colony-stimulating factor (rhG-CSF) was used for stem cell mobilization in 42 and 49 patients, respectively. The number of mononuclear cells (MNC) and CD34 + cells collected after mobilization, the maximum absolute neutrophil count (mANC), the cost of mobilization, and the engraftment time of white blood cells and platelets after transplantation were compared between the two groups. Results:The median number of MNC collected after mobilization in the PEG-rhG-CSF group and rhG-CSF group were 5.86×10 8/kg [(1.08-24.54)×10 8/kg] and 6.61×10 8/kg [(0.83-33.80)×10 8/kg], and the difference was not statistically significant ( U = 883.00, P = 0.245); while the median number of CD34 + cells collected after mobilization in the PEG-rhG-CSF group was higher than that in the rhG-CSF group [5.56×10 6/kg (0.94-19.90)×10 6/kg and 4.82×10 6/kg (1.12-14.61)×10 6/kg], and the difference was statistically significant ( U = 732.00, P = 0.038). The median number of mANC during mobilization in the PEG-rhG-CSF group was lower than that in the rhG-CSF group [20.50×10 9/L (7.26-61.30)×10 9/L and 32.08×10 9/L (6.92-69.99)×10 9/L], and the difference was statistically significant ( U = 490.00, P = 0.001). After autologous stem cell transplantation (ASCT), the time-to-recovery of white blood cell count (WBC) to 1.0×10 9/L in the PEG-rhG-CSF group was shorter than that in the rhG-CSF group [(11.59±1.98) d vs. (12.93±2.83) d], and the difference was statistically significant ( t = -2.395, P = 0.019), and the time-to-recovery of platelet count (Plt) to 20.0×10 9/L in the PEG-rhG-CSF group was also shorter than that in the rhG-CSF group [(12.86±2.62) d vs. (14.80±5.47) d], but the difference was not statistically significant ( t = -1.749, P = 0.085). The total mobilization cost of the PEG-rhG-CSF group was not statistically different from that of the rhG-CSF group [(21 405.47±7 365.98) yuan vs. (22 976.83±10 264.34) yuan, t = -0.721, P = 0.474]. Conclusions:PEG-rhG-CSF combined with high-dose chemotherapy is an effective option for PBSCM in MM patients, and its mobilization cost is equivalent to rhG-CSF. Therefore, PEG-rhG-CSF may be a better choice for PBSCM in MM patients.

5.
Article in Chinese | WPRIM | ID: wpr-882665

ABSTRACT

Objective:To explore the value of stress hyperglycemia ratio (SHR) in predicting the prognosis of patients with acute heart failure (AHF).Methods:AHF patients admitted to the Emergency Department of Shanghai Jiao Tong University Affiliated Sixth People's Hospital from December 2016 to January 2019 were retrospectively included. Clinical data were recorded and SHR was calculated. According to the survival of the patients within 1 year, they were divided into the death group ( n=89) and the survival group ( n=218). Logistic regression analysis was used to analyze the risk factors of mortality. Kaplan-Meier analysis was used to evaluate the correlation between SHR and the prognosis of AHF patients. Results:A total of 307 patients aged 83 ( range 74-87) years old who met the inclusion criteria were included in this study, including 153 males and 104 females. The age, SHR and N-terminal prohormone of brain natriuretic peptide (NT-proBNP) in the death group were higher than those in the survival group [84 (78, 88) vs 82 (72, 86), 1.11 (0.91, 1.51) vs 1.02 (0.86, 1.27), 5 351 (2 098, 14 039)μg/L vs 4 243 (2 294, 7 565)μg/L ]. The left ventricular ejection fraction (LVEF) in the death group was significantly lower than that of the survival group [53 (45, 57) % vs 58 (44, 64) %, P< 0.05]. Logistic regression analysis showed that SHR was an independent risk factor for death in AHF patients ( OR=2.397, 95% CI: 1.285-4.471, P< 0.05). Median SHR was used to draw the survival curve. Patients with high SHR had a lower cumulative survival rate, and the difference was statistically significant ( P<0.05). Conclusion:SHR can identify critically ill patients and is an independent risk factor for death in AHF patients.

6.
Article in Chinese | WPRIM | ID: wpr-863748

ABSTRACT

Objective To investigate the relationships between serum osteocalcin (OC) levels and glycometabolism markers in nondiabetic post-traumatic male patients.Methods Populaitons were selected at the Department of Emergency Medicine of Shanghai Jiao Tong University Affiliated Sixth People's Hospital from October 2017 to February 2019.The age,injury severity score (ISS),and characteristic indicators were recorded.The inclusion criteria were age ≥ 18 years and blood collection time < 24 h after the injury.The exclusion criteria were emergency surgery,acute brain trauma,and hemoglobin A1c (HbA1c) ≥ 6.0%.The patients were divided into two groups by fasting plasma glucose (FPG):stress hyperglycemia (SH) (FPG>7.8 mmol/L) and nonstress hyperglycemia (NO-SH) (FPG ≤ 7.8 mmol/L) groups.The fasting venous blood samples were collected and examined.The characteristics and biochemical indicators in the two groups were compared statistically by LSD-t test,rank sum test and ANOVA,and the relationships between serum OC levels and glycometabolism markers were analyzed by partial correlation analysis.Results A total of 395 traumatic patients were enrolled and divided into the SH group (n=182) and NO-SH group (n=213).There were no differences in ISS,fasting insulin (FINS),and C-peptide (C-P) levels between groups.Age,HbAlc and FPG were higher (P=0.041,P=0.037,P<0.01),while the OC level was lower (P=0.023),in the SH group than those in the NO-SH group.The serum OC level did not correlate with HbAlc,FPG,and FINS,but negatively correlated with C-P by partial correlation analysis (r=-0.262,P=0.008).The multivariate linear regression analysis showed that C-P was an independent factor affecting serum OC levels after trauma (β=-0.655,P=0.043).Conclusion A correlation existed between the serum OC level and glycometabolism markers in nondiabetic post-traumatic male patients.

7.
Article in Chinese | WPRIM | ID: wpr-862796

ABSTRACT

Objective:To investigate the clinical features, diagnosis, occurrence sequence and clonal origin of chronic lymphocytic leukemia complicated with multiple myeloma.Methods:The diagnosis and treatment of one patient with multiple myeloma and chronic lymphocytic leukemia who was admitted to the First Hospital of Jilin University in May 2018 was retrospectively analyzed, and the related literatures were reviewed.Results:This patient began with lumbosacral pain, and he was diagnosed as chronic lymphocytic leukemia complicated with multiple myeloma after bone marrow aspiration, flow cytometry, and blood and urine immunofixation electrophoresis. It is recommended that Rd (lenalidomide + dexamethasone) or MPV (melphalan + prednisone + bortezomib) regimen, but the patient did not receive chemotherapy and died of infectious diarrhea 1 month later.Conclusions:The occurrence of multiple myeloma and chronic lymphoblastic leukemia may originate from the same clone or different new clone. It is very rare that multiple myeloma and chronic lymphoblastic leukemia can co-occur. Therapeutic options tend to be more aggressive multiple myeloma-based regimen.

8.
Article in Chinese | WPRIM | ID: wpr-828099

ABSTRACT

OBJECTIVE@#To establish an algorithm based on 3D convolution neural network to segment the organs at risk (OARs) in the head and neck on CT images.@*METHODS@#We propose an automatic segmentation algorithm of head and neck OARs based on V-Net. To enhance the feature expression ability of the 3D neural network, we combined the squeeze and exception (SE) module with the residual convolution module in V-Net to increase the weight of the features that has greater contributions to the segmentation task. Using a multi-scale strategy, we completed organ segmentation using two cascade models for location and fine segmentation, and the input image was resampled to different resolutions during preprocessing to allow the two models to focus on the extraction of global location information and local detail features respectively.@*RESULTS@#Our experiments on segmentation of 22 OARs in the head and neck indicated that compared with the existing methods, the proposed method achieved better segmentation accuracy and efficiency, and the average segmentation accuracy was improved by 9%. At the same time, the average test time was reduced from 33.82 s to 2.79 s.@*CONCLUSIONS@#The 3D convolution neural network based on multi-scale strategy can effectively and efficiently improve the accuracy of organ segmentation and can be potentially used in clinical setting for segmentation of other organs to improve the efficiency of clinical treatment.


Subject(s)
Head , Humans , Image Processing, Computer-Assisted , Neck , Neural Networks, Computer , Organs at Risk , Tomography, X-Ray Computed
9.
China Pharmacy ; (12): 1809-1815, 2020.
Article in Chinese | WPRIM | ID: wpr-823349

ABSTRACT

OBJECTIVE:To investigate the effects of gypenosides (GPs)on gene expression of major urinary proteins (Mups) in liver tissue of hypercholesterolemia model mice. METHODS :C57BL/6J mice were divided into control (ND)group,model (HFD)group and GPs therapy (GP)group according to body weight (BW),with 11 mice in each group. Except for ND group , other groups were given high-lipid diet to induce hypercholesterolemia model. From the 17th week of feeding ,ND group and HFD group were given constant volume of 0.1%CMC-Na solution intragastrically ;GP group were given GPs suspension (250 mg/kg) intragastrically,once a day ,for consecutive 22 weeks. BW ,the levels of blood glucose (BG)and blood lipid (TC,LDL-C)were detected in each group. Total RNA of liver tissue was extracted ,and reverse transcription library was constructed and RNA-seq sequencing was performed. The differentially expressed genes were screened by PCA ,volcano map and scatter plot. RT-qPCR was used for verification for differentially expressed genes. The correlation between the expression of differentially expressed genes and the above pharmacodynamic indexes was analyzed by bivariate analysis. RESULTS :Compared with ND group ,BW,the levels of BG,TC and LDL-C in HFD group were increased significantly (P<0.05). Compared with HFD group ,above indexes of GP group were decreased significantly except for BW (P<0.05). PCA showed that the data of ND group and HFD group distributed in different quadrants ,and the data distribution of GP group was between above two groups. mRNA of Mup4,Mup5,Mup11,Mup15 and Mup21 in liver tissue of mice were increased significantly after treated with high-fat diet (P<0.05). mRNA of Mup3,Mup4, Mup5,Mup8,Mup12 and Mup21 were decreased significantly after treated with GPs (P<0.05). In ND group vs. HFD group and HFD group vs. GP group ,mRNA of Mup4,Mup5 and Mup21 genes changed significantly and the trend was opposite. Results of RT-qPCR verification showed that compared with ND group ,relative mRNA expression of Mup4,Mup5 and Mup21 gene were increased significantly in HFD group (P<0.05). Correlation analysis revealed that mRNA expression of Mup5 was positively correlated with the levels of TC and BG (r=0.727 1,0.670 6,P<0.05),mRNA expression of Mup4 and Mup21 were positively correlated with the level of BG (r=0.737 8,0.721 5,P<0.05). CONCLUSIONS :GPs can regulate the expression of Mups genes in liver tissue of hypercholesterolemia model mice , and reduce glucose and lipid level through regulating the mRNA over-expression of Mup4,Mup5 and Mup21.

10.
Article in Chinese | WPRIM | ID: wpr-799292

ABSTRACT

Objective@#To investigate the clinical features, diagnosis, occurrence sequence and clonal origin of chronic lymphocytic leukemia complicated with multiple myeloma.@*Methods@#The diagnosis and treatment of one patient with multiple myeloma and chronic lymphocytic leukemia who was admitted to the First Hospital of Jilin University in May 2018 was retrospectively analyzed, and the related literatures were reviewed.@*Results@#This patient began with lumbosacral pain, and he was diagnosed as chronic lymphocytic leukemia complicated with multiple myeloma after bone marrow aspiration, flow cytometry, and blood and urine immunofixation electrophoresis. It is recommended that Rd (lenalidomide + dexamethasone) or MPV (melphalan + prednisone + bortezomib) regimen, but the patient did not receive chemotherapy and died of infectious diarrhea 1 month later.@*Conclusions@#The occurrence of multiple myeloma and chronic lymphoblastic leukemia may originate from the same clone or different new clone. It is very rare that multiple myeloma and chronic lymphoblastic leukemia can co-occur. Therapeutic options tend to be more aggressive multiple myeloma-based regimen.

11.
Journal of Preventive Medicine ; (12): 1108-1111, 2019.
Article in Chinese | WPRIM | ID: wpr-815954

ABSTRACT

Objective@#To learn the characteristics of gastric cancer incidence and mortality in Lishui from 2014 to 2018,and to provide basis for prevention and control strategies for gastric cancer. @*Methods@#The data of gastric cancer in Lishui from 2014 to 2018 was collected from Zhejiang Chronic Disease Surveillance Information Management System. The incidence and mortality rates of gastric cancer were calculated,and the trend of them was evaluated by annual percentage change(APC).@*Results@#The crude incidence rate of gastric cancer in Lishui was 44.77/100 000(5 650 cases)and the standardized one was 26.93/100 000. From 2014 to 2018,the incidence rates of gastric cancer showed a decreasing trend(APC=-0.79%,P<0.05). The crude mortality rate of gastric cancer in Lishui was 25.39/100 000(3 205 cases)and the standardized one was 14.21/100 000. From 2014 to 2018,the mortality rates of gastric cancer were stable(APC=-4.61%,P>0.05). The standardized incidence and mortality rates of gastric cancer in males were 34.77/100 000 and 18.35/100 000,which were higher than 16.77/100 000 and 8.52/100 000 in females(P<0.05). The standardized incidence and mortality rates of gastric cancer in rural residents were 46.07/100 000 and 26.11/100 000,which were higher than 38.49/100 000 and 22.02/100 000 in urban residents. The incidence and mortality rates increased with age after 15 years old,and reached the peak at the age group of 80-84 years old.@*Conclusion@#The incidence and mortality of gastric cancer in Lishui from 2014 to 2018 were at a high level,which were higher in males than in females and were higher in rural than in urban areas;while the incidence had a downward trend.

12.
Journal of Preventive Medicine ; (12): 325-329, 2019.
Article in Chinese | WPRIM | ID: wpr-815767

ABSTRACT

Objective @#To investigate the knowledge,attitude and practice of salt reduction for hypertension prevention among residents in Liandu District of Lishui,and to provide reference for intervention of salt reduction.@*Methods @#Residents aged 18-69 years in two communities and three towns of Liandu District were selected by multistage stratified random sampling method. They were investigated the prevalence of hypertension and knowledge,attitude and practice of salt reduction for hypertension prevention. Logistic regression models were used to analyze the influencing factors for knowledge,attitude and practice of salt reduction for hypertension prevention.@*Results @#Among 1 801 participants,1 509 completed the survey,with a response rate of 83.79%. The prevalence of hypertension was 31.88%. The proportions of the participants who had knowledge, attitude and practice of salt reduction for hypertension prevention were 33.40%,60.70% and 58.58%,respectively. The results of multivariate logistic regression analysis showed that women(OR=1.583,95%CI:1.248-2.008),hypertension(OR= 1.734,95%CI:1.303-2.308),30 to 39 years old(OR=0.368,95%CI:0.226-0.597),junior high school and above education(OR=0.057-0.403,95%CI:0.036-0.577)were the influencing factors for the knowledge of salt reduction for hypertension prevention;women(OR=1.342,95%CI:1.072-1.679),married(OR=1.582,95%CI:1.167-2.144),junior or senior high school education(OR=0.235-0.525,95%CI:0.163-0.736),having the knowledge(OR=2.640,95%CI:2.033-3.430)were the influencing factors for the attitude of salt reduction for hypertension prevention;women(OR=1.632,95%CI:1.304-2.044),30 to 39 years old(OR=0.494,95%CI:0.346-0.705),rural residents(OR= 1.617,95%CI:1.276-2.050),family history of chronic diseases(OR=1.455,95%CI:1.148-1.845),having the knowledge(OR= 1.396,95%CI:1.084-1.797),having the attitude(OR=4.059,95%CI:3.203-5.145)were the influencing factor for the practice of salt reduction for hypertension prevention.@*Conclusion @#The residents in Liandu District had low levels of the knowledge,attitude and practice of salt reduction for hypertension prevention. The higher levels of the knowledge and attitude of salt reduction for hypertension prevention,the higher level of the practice.

13.
Journal of Preventive Medicine ; (12): 669-672, 2019.
Article in Chinese | WPRIM | ID: wpr-815678

ABSTRACT

Objective@#To investigate the sodium,potassium and blood pressure of adult residents in Liandu District of Lishui,and to provide evidence for salt reduction intervention.@*Methods@#A stratified random sampling method was used to select residents aged 18 to 69 years in Liandu District from December 2016 to February 2017. A questionnaire survey and physical examination were conducted to collect basic information. The 24-hour urine samples were collected to detect sodium and potassium intake.@*Results@#Among 300 participants,the average daily intake of sodium was(10.59±3.90)g,which was higher in men than in women [(11.11±4.33)g vs.(10.08±3.37)g,P<0.05]. There were 274 participants with average daily intake of sodium more than 6 g,accounting for 91.33%. The average daily intake of potassium was(1.67±0.71)g,which was higher in women than in men [(1.77±0.72)g vs.(1.57±0.68)g,P<0.05]. The average daily intakes of potassium were significantly different among different age groups,with participants aged 20 to 29 years the lowest(P<0.05). There were 217 participants with average daily intake of potassium less than 2 g,accounting for 72.33%. The ratio of sodium to potassium was 4.7±2.2,which was higher in men than in women(5.3±2.7 vs. 4.1±1.4,P<0.05)and decreased with age(P<0.05). There were 291 participants with the ratio more than 1.87,accounting for 97.00%. The prevalence of hypertension was 31.67%,which was 33.65% in men and 29.80% in women.@*Conclusion@#The residents in Liandu District have high intake of sodium and prevalence of hypertension,yet low intake of potassium.

14.
Cancer Research and Clinic ; (6): 354-357, 2019.
Article in Chinese | WPRIM | ID: wpr-756756

ABSTRACT

In tumor immunotherapy, the study of programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) provides a new approach for the comprehensive treatment of advanced lung cancer. About 20% of patients with non-small cell lung cancer could benefit from lung cancer immunotherapy, while those with high PD-L1 expression will benefit more. At present, there are few related studies on PD-1 expression at home and abroad, and the detection of PD-1/PD-L1 expressions is mostly concentrated in tumor tissues. With the research progress of liquid biopsy technology, the convenience and accuracy of peripheral blood testing are also receiving more and more attention. However, there are still few biological indicators for predicting the efficacy of tumor immunotherapy, and the uniform standard and accuracy of testing still need more clinical practice and exploration. This article reviews the research on the expressions of PD-1 and PD-L1 in tissues and peripheral blood of patients with lung cancer, aiming to provide reference for the treatment of lung cancer with immune checkpoint blockers.

15.
Article in Chinese | WPRIM | ID: wpr-751890

ABSTRACT

Objective To explore the relationship between serum apolipoprotein A-1 (apoA-1) level and severity of trauma in patients with trauma.Methods Data of 138 trauma patients admitted by EICU from October 2017 to April 2018 in the Acute Trauma Emergency Center of the Sixth People's Hospital Affiliated to Shanghai Jiaotong University were collected retrospectively. Gender, age, admission time, trauma mechanism, and injury severity score (ISS) were recorded, and serum apoA-1 level was measured and recorded within 24 h after injury. Patients were divided into three groups according to the ISS score: the light injury group (47 cases, ISS < 16), the serious injury group (45 cases, 16≤ISS < 25), and the critical injury group (46 cases, ISS≥ 25). The correlation between serum apoA-1 level and ISS score was verified by adopting Pearson correlation analyses, and the differences in serum lipoprotein A level within 24 h after injury were compared among 3 groups by one-way ANOVA. Results Serum apoA-1 level was negatively correlated with ISS score (r=-0.307,P<0.01). There was significant difference in serum apoA-1 level between different trauma groups (F=8.864,P<0.01), among which the difference between the light injury group and critical injury group was the largest (P<0.01). The level of serum apoA-1 in the critical injury group was lower than that in the light injury group (P<0.01) and the serious injury group (P=0.038), while the level of serum apoA-1 in the serious injury group was lower than that in the light injury group (P=0.040).Conclusions Early serum apoA-1 level in patients with trauma is closely related to the severity of trauma and may be a better indicator to evaluate the prognosis of patients with trauma.

16.
Article in Chinese | WPRIM | ID: wpr-745888

ABSTRACT

The clinical presentation,chest image and electrocardiogram of 4 cases of premature complex-induced chronic cough admitted in the First Affiliated Hospital of Guangzhou Medical University from March 2008 to February 2009,were retrospectively analyzed.And other 6 cases of premature complex-induced chronic cough were retrieved for literature review.In total 10 patients,there were 3 males and 7 females aged 58(36-74) years,with a disease duration of 10.5 (3.0,264.0) months.The main clinical presentations were chronic dry cough with post-sternal thump and premature beats as shown by auscultation and 24 h-electrocardiogram examination.Cough was relieved after the premature beats were controlled with the treatment of arrhythmia drugs or radiofrequency ablation.Premature complex-induced cough is rare but it should be considered when the chief complaint of patients with premature beat is chronic cough.

17.
Clinical Medicine of China ; (12): 209-212, 2019.
Article in Chinese | WPRIM | ID: wpr-744985

ABSTRACT

Objective To explore the correlation between NLR family pyrin domain containing 3 (NLRP3) and nonalcoholic fatty liver disease (NALFD).Methods A cross-sectional study was conducted in this study.Eighty four cases of health examination personnel in Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital were included in the study randomly,and divided into NAFLD group (27cases) and normal control group (57cases) though liver ultrasound examination.Their general data (sex,height,weight),liver function,blood lipid,and blood glucose were analyzed.The level of NLRP3 in peripheral blood was analyzed by ELISA double antibody sandwich method.The relationship between NLRP3 and NAFLD was analyzed by Logistic regression.Results The prevalence of NAFLD was 32.1 % (27/84).The levels of serum cholesterol,triglyceride,low density lipoprotein cholesterol,fasting blood sugar,alanine aminotransferase,creatinine and uric acid in NAFLD group were higher than those in normal control group (all P<0.01),while the level of serum high density lipoprotein cholesterol was lower than that in normal control group (P=0.023);the concentration of serum NLRP3 in NAFLD group was (5.1±1.8) μg/L,and that in normal control group was (3.9±1.4) μg/L.There was significant difference between the two groups (t =6.221,P =0.016).Logistic regression analysis showed that NLRP3 was a risk factor for NAFLD (OR =1.537,P =0.021).Conclusion The level of NLRP3 is up-regulated in the serum of NAFLD patients,which is related to the occurrence of NAFLD.

18.
Chinese Journal of Hematology ; (12): 644-649, 2019.
Article in Chinese | WPRIM | ID: wpr-805797

ABSTRACT

Objectives@#To evaluate the clinical characteristics and prognosis of high risk cytogenetic abnormalities (HRCA) and various combinations of cytogenetic abnormality in patients with newly-diagnosed multiple myeloma (NDMM) .@*Methods@#This retrospective study collected 182 NDMM patients in the First Affiliated Hospital of Jilin University between Nov. 2009 and May 2018. HRCA included 1q+, del (17p) , t (4;14) , and t (14;16) detected by FISH, and non-HRCA included del (13q) , t (11;14) detected by FISH. The clinical characteristics among three groups, including cases who carrying a single HRCA, 1 HRCA in combination with non-HRCA and cases carrying two or more HRCAs (double/triple-hit) were observed. Kaplan-Meier curve was used to analyze both progression-free survival (PFS) and overall survival (OS) for the three groups.@*Results@#The survivals of patients with 1 HRCA in combination with non-HRCA were similar to those with two or more HRCAs (double/triple-hit) , the median PFS (mPFS) was 19.1 m vs 12.1 m (P=0.248) and median OS (mOS) was 29.6 m vs 29.3 m (P=0.774) . Furthermore, the prognosis of these two groups were both inferior to patients with a single HRCA, respectively. (mPFS: 32.2 m, P=0.040, P=0.001; mOS: 42.3 m, P=0.021, P=0.041) . Strikingly, both the mPFS and the mOS of patients with 1 HRCA in combination with non-HRCA (regardless of high risk or not) were significantly shorter than that of cases with a single HRCA (mPFS: 15.1 m vs 32.2 m, HR=2.126, 95%CI 1.176-3.843, P=0.005; mOS: 29.3 m vs 42.3 m, HR=1.442, 95%CI 0.705-2.950, P=0.011) .@*Conclusion@#It is of prognostic significance value for detecting double/triple-hit based on FISH cytogenetics in NDMM.

19.
Chinese Journal of Hematology ; (12): 584-588, 2019.
Article in Chinese | WPRIM | ID: wpr-805658

ABSTRACT

Objective@#To evaluate the prognostic value of kinetic changes in minimal residual disease (MRD) status, as well as its relationship with risk stratification, therapeutic response and treatment in patients with newly-diagnosed multiple myeloma (MM) .@*Methods@#A total of 135 patients with newly-diagnosed MM were screened, and 105 patients who achieved VGPR or more as the best responses were included into this study. The MRD status was determined by multiparameter flow cytometry (MFC) at multiple intervals after two cycles of treatment until clinical relapse, death, or last follow-up. The statistical methods included Kaplan-Meier analysis, Cox regression, etc.@*Results@#①In all 135 patients, 57.8% (78/135) patients achieved MRD negativity (MRD-) after treatment. In 105 patients who achieved VGPR and thus included in this study, the MRD- rate was 72.4% (76/105) , with a median interval of 3 months from starting treatment to achievement of MRD- status. ②The 2-year PFS rate of patients with MRD- status was significantly higher than that of MRD+ status (62.2% vs 41.3%, P=0.001) , while MRD persistence (MRD+) was an independent factor for poor prognosis (multivariate analysis for PFS: P=0.044, HR=3.039, 95%CI 1.029-8.974) . ③Loss of MRD- status (i.e., MRD reappearance) showed inferior outcomes compared with MRD sustained negative ones, the PFS was 18 months versus not reach (P<0.001) and the OS was not reach for both (P=0.002) . ④The 2-year PFS and OS rates of patients with duration of MRD-status≥12 months were significantly higher than those of the control group (PFS: 77.7% vs 36.7%, P<0.001; OS: 96.4% vs 57.9%, P<0.001 respectively) . Duration of MRD- status was associated with a marked reduction in risk of relapse or death (univariate analysis for PFS: P<0.001, HR=0.865, 95%CI 0.815-0.918; for OS: P=0.001, HR=0.850, 95%CI 0.741-0.915 respectively) . ⑤Moreover, even in patients carrying high-risk cytogenetic abnormalities (CA) or ineligible for ASCT, MRD negativity remained its prognostic value to predict PFS (high-risk CA medianPFS: not reach vs 19 months, P=0.006; ineligible for ASCT medianPFS: not reach vs 25 months, P=0.052 respectively) . ⑥Last, treatment with the bortezomib-based regimens contributed to prolonged MRD- duration (median MRD- duratio: 25 months vs 10 months, P=0.034) .@*Conclusion@#Our findings supported MRD+ status as an independent poor prognostic factor in MM patients, which implicated that duration of MRD- status also played a significant role in evaluation of prognosis, while loss of MRD-status might serve as an early biomarker for relapse. Therefore, monitoring of MRD kinetics might more precisely predict prognosis, as well as guide treatment decision, especially for when to start retreatment in relapsed patients.

20.
Chinese Journal of Hematology ; (12): 912-917, 2019.
Article in Chinese | WPRIM | ID: wpr-801365

ABSTRACT

Objective@#To evaluate the prognostic significance of combining ISS-Ⅲ and high risk cytogenetic abnormalities [HRCAs, including 1q gain/amplification and del (17p) ] in patients with newly-diagnosed multiple myeloma (NDMM) .@*Methods@#The clinical characteristics and relevant variables were retrospectively analyzed in a total of 270 NDMM patients diagnosed between November 2009 and May 2018. ISS-Ⅲ stage and HRCAs [detected by FISH, including 1q gain/amplification and del (17p) ] were defined as risk factors (hit) . Based to the number of hit per case, these patients were divided into four groups carrying 0 to 3 risk factors, respectively. Progress-free survival (PFS) and overall survival (OS) were then analyzed using the Kaplan-Meier estimator.@*Results@#Patients who carried single hit (n=120, 44.4%) had shorter median PFS (23.0 vs 28.9 months; P>0.05) and OS (42.3 vs 53.7 months; P>0.05) than those with no risk factors (n=66, 24.4%) . Of note, the outcome of patients who had two or more risk factors (double/triple, n=84, 31.1%) was much worse than those with either no or one risk factor, indicated by significantly reduced median PFS (14.5 months; HR=1.584, 95%CI 1.082-2.319; P=0.003 for double/triple vs single hit) and OS (18.4 months, HR=2.299, 95%CI 1.485-3.560; P<0.001 for double/triple vs single hit) . Strikingly, patients who had three risk factor (triple hit, n=5, 1.9%) displayed the poorest survival with extraordinarily shorter PFS (0.9-15.1 months) and OS (0.9-18.9 months) compared to those carrying two risk factors (double hit) . Analogous results were obtained when different combinations of ISS stages and HRCAs were analyzed.@*Conclusion@#These results suggest a potential but rather important role of combining multiple (e.g. double or triple) adverse factors determined via the routine ISS staging and FISH detection of cytogenetic abnormalities in risk stratification and prognostic prediction, which might be helpful to identify high risk patients more precisely at diagnosis. It also raised a possibility that a small group of ISS-Ⅲ patients carrying both 1q gain/amplification and del (17p) might represent an "extremely-high risk" subset of MM.

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