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1.
Article in English | WPRIM | ID: wpr-922685

ABSTRACT

Porphyromonas gingivalis (P. gingivalis), a key pathogen in periodontitis, has been shown to accelerate the progression of atherosclerosis (AS). However, the definite mechanisms remain elusive. Emerging evidence supports an association between mitochondrial dysfunction and AS. In our study, the impact of P. gingivalis on mitochondrial dysfunction and the potential mechanism were investigated. The mitochondrial morphology of EA.hy926 cells infected with P. gingivalis was assessed by transmission electron microscopy, mitochondrial staining, and quantitative analysis of the mitochondrial network. Fluorescence staining and flow cytometry analysis were performed to determine mitochondrial reactive oxygen species (mtROS) and mitochondrial membrane potential (MMP) levels. Cellular ATP production was examined by a luminescence assay kit. The expression of key fusion and fission proteins was evaluated by western blot and immunofluorescence. Mdivi-1, a specific Drp1 inhibitor, was used to elucidate the role of Drp1 in mitochondrial dysfunction. Our findings showed that P. gingivalis infection induced mitochondrial fragmentation, increased the mtROS levels, and decreased the MMP and ATP concentration in vascular endothelial cells. We observed upregulation of Drp1 (Ser616) phosphorylation and translocation of Drp1 to mitochondria. Mdivi-1 blocked the mitochondrial fragmentation and dysfunction induced by P. gingivalis. Collectively, these results revealed that P. gingivalis infection promoted mitochondrial fragmentation and dysfunction, which was dependent on Drp1. Mitochondrial dysfunction may represent the mechanism by which P. gingivalis exacerbates atherosclerotic lesions.


Subject(s)
Endothelial Cells , Mitochondria , Mitochondrial Dynamics , Porphyromonas gingivalis
2.
Article in Chinese | WPRIM | ID: wpr-755698

ABSTRACT

Objective To study the effect of N-Methyl-D-asparticacid ( NMDA ) on the intracellular free calcium concentration ( [ Ca2+] i ) in primary cultured rat calvaria osteoblasts. Methods A calcium imaging technique was applied to observe [ Ca2+] i changes in primary cultured rat calvaria osteoblasts after stimulating by NMDA with various concentrations or pretreated with NMDA receptor noncompetitive antagonism MK801 ( Dizocilpin) . Results Different concentrations of NMDA caused [ Ca2+] i increases in varying degrees and by different ways. NMDA could evoke transient increase and secondary change in [ Ca2+] i including calcium oscillation or steady increase. MK801 inhibited NMDA-induced [ Ca2+] i increase in varying degrees. Conclusion These results indicated that there are abundant functional NMDA receptors expressed in primary cultured rat calvaria osteoblasts, showing different forms and varying degrees of [ Ca2+] i increases in response to different concentrations of NMDA. The characters of the blocking effect of MK801 to NMDA-induced [ Ca2+] i increasing, indicated that the NMDA receptors expressed in primary cultured rat calvaria osteoblasts differ in channel properties from those in central nervous system.

3.
Article in Chinese | WPRIM | ID: wpr-752595

ABSTRACT

Objective To discuss the effect of self-efficacy on patients with coronary heart disease cured in general practice department based on Hospital-Community-Patient Integrated Nursing Mode. Methods From January to April in 2018, 106 patients (51 males and 55 females) with coronary heart disease hospitalized in general practice of hospital were selected as subjects of study. Random number table method was used to divide the patients into control group and intervention group, 53 cases in each group. The intervention group adopted the hospital-community-patient integrated nursing model, while the control group adopted the traditional health education mode after discharge. Self-efficacy evaluation was conducted before intervention, 3 months after intervention and 6 months after intervention. Results The total score of self-efficacy in the two groups was higher than that before intervention, but the increase in the intervention group was significantly better than that in the control group, the difference was statistically significant (F=34.681, P < 0.01). The total scores of self-efficacy in intervention group were (30.35 ± 2.58), (33.59 ± 2.68) points respectively 3 months and 6 months after intervention, which were higher than (28.95 ± 2.42), (29.10 ± 2.12) points in control group. The difference was significant (t =3.702, 13.494, P<0.01). Conclusion Hospital-community-patient integrated nursing model is superior to traditional health education model after discharge, which can significantly improve the self-efficacy of patients with coronary heart disease discharged from general practice department.

4.
Article in English | WPRIM | ID: wpr-763720

ABSTRACT

The retained functionality of the sodium iodide symporter (NIS) expressed in differentiated thyroid cancer (DTC) cells allows the further utilization of post-surgical radioactive iodine (RAI) therapy, which is an effective treatment for reducing the risk of recurrence, and even the mortality, of DTC. Whereas, the dedifferentiation of DTC could influence the expression of functional NIS, thereby reducing the efficacy of RAI therapy in advanced DTC. Genetic alternations (such as BRAF and the rearranged during transfection [RET]/papillary thyroid cancer [PTC] rearrangement) have been widely reported to be prominently responsible for the onset, progression, and dedifferentiation of PTC, mainly through activating the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) signaling cascades. These genetic alternations have been suggested to associate with the reduced expression of iodide-handling genes in thyroid cancer, especially the NIS gene, disabling iodine uptake and causing resistance to RAI therapy. Recently, novel and promising approaches aiming at various targets have been attempted to restore the expression of these iodine-metabolizing genes and enhance iodine uptake through in vitro studies and studies of RAI-refractory (RAIR)-DTC patients. In this review, we discuss the regulation of NIS, known mechanisms of dedifferentiation including the MAPK and PI3K pathways, and the current status of redifferentiation therapy for RAIR-DTC patients.


Subject(s)
Humans , In Vitro Techniques , Iodine , Ion Transport , Isotopes , Mortality , Protein Kinases , Recurrence , Sodium Iodide , Thyroid Gland , Thyroid Neoplasms , Transfection
5.
Article in Chinese | WPRIM | ID: wpr-802752

ABSTRACT

Objective@#To discuss the effect of self-efficacy on patients with coronary heart disease cured in general practice department based on Hospital-Community-Patient Integrated Nursing Mode.@*Methods@#From January to April in 2018, 106 patients (51 males and 55 females) with coronary heart disease hospitalized in general practice of hospital were selected as subjects of study. Random number table method was used to divide the patients into control group and intervention group, 53 cases in each group. The intervention group adopted the hospital-community-patient integrated nursing model, while the control group adopted the traditional health education mode after discharge. Self-efficacy evaluation was conducted before intervention, 3 months after intervention and 6 months after intervention.@*Results@#The total score of self-efficacy in the two groups was higher than that before intervention, but the increase in the intervention group was significantly better than that in the control group, the difference was statistically significant (F=34.681, P < 0.01). The total scores of self-efficacy in intervention group were(30.35±2.58), (33.59±2.68) points respectively 3 months and 6 months after intervention, which were higher than (28.95±2.42), (29.10±2.12) points in control group. The difference was significant (t = 3.702, 13.494, P < 0.01).@*Conclusion@#Hospital-community-patient integrated nursing model is superior to traditional health education model after discharge, which can significantly improve the self-efficacy of patients with coronary heart disease discharged from general practice department.

6.
Chinese Critical Care Medicine ; (12): 695-698, 2018.
Article in Chinese | WPRIM | ID: wpr-806824

ABSTRACT

Objective@#To investigate the efficacy of prussian blue (PB) or its combination with hemoperfusion (HP) in the treatment of acute thallium poisoning.@*Methods@#Forty-seven patients with acute thallium poisoning with complete data hospitalized in the 307th Hospital of PLA from September 2002 to December 2017 were enrolled, and they were divided into mild poisoning group (blood thallium < 150 μg/L, urinary thallium < 1 000 μg/L) and moderate-severe poisoning group (blood thallium ≥ 150 μg/L, urinary thallium ≥ 1 000 μg/L) according to the toxic degrees. All patients were given symptomatic supportive treatments such as potassium supplementation, catharsis, vital organ protections, neurotrophic drugs, and circulation support. The mild poisoning patients were given PB with an oral dose of 250 mg·kg-1·d-1, while moderate-severe poisoning patients were given PB combined HP continued 2-4 hours each time. The PB dose or frequency of HP application was adjusted according to the monitoring results of blood and urine thallium. Data of gender, age, pain grading (numeric rating scale NRS), clinical manifestations, blood and urine thallium before and after treatment, length of hospitalization and prognosis were collected.@*Results@#Of the 47 patients, patients with incomplete blood and urine test results, and used non-single HP treatment such as plasmapheresis and hemodialysis for treatment were excluded, and a total of 29 patients were enrolled in the analysis. ①Among 29 patients, there were 20 males and 9 females, median age of 40.0 (34.0, 49.0) years old; the main clinical manifestations were nervous system and alopecia, some patients had digestive system symptoms. There were 13 patients (44.8%) in the mild poisoning group with painless (grade 0) or mild pain (grade 1-3) with mild clinical symptoms, the length of hospitalization was 17.0 (14.2, 21.5) days. There were 16 patients (55.2%) in the moderate-severe poisoning group with moderate pain (grade 4-6) or severe pain (grade 7-10) with severe clinical symptoms, the length of hospitalization was 24.0 (18.0, 29.0) days. ② After treatment, the thallium concentrations in blood and urine in the mild poisoning group were significantly lower than those before treatment [μg/L: blood thallium was 0.80 (0, 8.83) vs. 60.00 (40.00, 120.00), urine thallium was 11.30 (0, 70.10) vs. 370.00 (168.30, 610.00), both P < 0.01], the thallium concentrations in blood and urine in the moderate-severe poisoning group were also significantly lower than those before treatment [μg/L: blood thallium was 6.95 (0, 50.50) vs. 614.50 (245.00, 922.00), urinary thallium was 20.70 (1.95, 283.00) vs. 5 434.00 (4 077.20, 10 273.00), both P < 0.01]. None of the 29 patients died, and their clinical symptoms were improved significantly. All the 27 patients had good prognosis without sequela in half a year follow-up, and 2 patients with severe acute thallium poisoning suffered from nervous system injury.@*Conclusion@#In the acute thallium poisoning patients, on the basis of general treatment, additional PB in mild poisoning group and PB combined with HP in moderate-severe poisoning group can obtain satisfactory curative effects.

7.
Article in Chinese | WPRIM | ID: wpr-708858

ABSTRACT

Objective To compare two recurrence-risk stratification software (RSS),which could evaluate the recurrence-risk in patients with differentiated thyroid carcinoma (DTC) intelligently.Methods Based on 2009 American Thyroid Association (ATA) guidelines and clinical guidelines for 131I therapy of DTC patients in China (2014),two RSS (RSS1 and RSS2) were designed.From January 2013 to January 2016,1 043 non-metastasis DTC patients (386 males,657 females;average age (46.4±10.5) years) in Peking Union Medical College Hospital were involved to be risk-stratified,and the results were evaluated by ATA response evaluation system.x2 test was used to analyze the data.Results With 2 years' (median) follow-up,the recurrence rates in low,intermediate and high recurrence-risk groups evaluated by RSS1 were 2.8%(1/36),4.7% (34/725) and 42.9% (121/282),and those were 0(0/29),3.7% (26/698) and 41.1%(130/316) evaluated by RSS2.The recurrence rate was lower in low-risk group evaluated by RSS2 than that by RSS1,but there was no significant difference (x2=3.046,P>0.05).More patients with recurrence were divided into high-risk group evaluated by RSS2,but the recurrence rates of 2 high-risk groups evaluated by RSS1 and RSS2 were not significantly different (x2 =0.082,P>0.05).Conclusion RSS1 and RSS2 could predict recurrence-risk effectively,and RSS2 could classify more recurrent patients into high-risk group.

8.
Article in Chinese | WPRIM | ID: wpr-706243

ABSTRACT

Objective To explore the value of 3.0T MR elastography (MRE) in diagnosis of obstructive chronic pancreatitis.Methods Totally 32 patients (lesion group) with suspected obstructive chronic pancreatitis who underwent pancreaticoduodenectomy and 32 volunteers (normal control group) were enrolled.MRE was performed,and pancreatic stiffness value was measured.The consistency between two observers and the repeatability of the same observer were evaluated.The difference of pancreatic stiffness value was compared between the two groups.The efficacy of pancreatic stiffness value in diagnosis of obstructive chronic pancreatitis was analyzed with ROC curve.Results The consistency between two observers and the repeatability of the same observer were excellent (all ICC>0.9).The pancreatic stiffness value of normal control group and lesion group was (1.21±0.11)kPa and (1.51±0.24)kPa,respectively (t=-6.077,P <0.001).The area under ROC curve of pancreatic stiffness value in diagnosis of chronic pancreatitis,mild and moderate to severe,mild to moderate and severe was 0.900,0.941 and 0.960,respectively (all P<0.001).Conclusion MRE can objectively measure pancreatic stiffness and noninvasively assess the severity of chronic pancreatitis.

9.
Article in Chinese | WPRIM | ID: wpr-706181

ABSTRACT

Objective To explore the value of MR elastography (MRE) and dynamic contrast-enhanced imaging (DCE-MRI) in diagnosis of gastroesophageal varices (GEV) in patients with liver cirrhosis.Methods Totally 59 patients with liver cirrhosis underwent MRE and DCE-MRI.Taking endoscopic examination as the standard,platelet (PLT) count,hepatic stiffness (HS),spleen stiffness (SS) and MR visual score (MR VS) were measured.Values of related parameters in diagnosis of liver cirrhosis GEV were compared with area under the curve (AUC).Results PLT,HS,SS and MR VS were significantly correlated with the grades of GEV with liver cirrhosis (rs =-0.317,0.436,0.682,0.703,all P<0.05).In the diagnosis of with or without GEV,AUC of SS was higher than that of MR VS,HS,and PLT (AUC=0.880,0.795,0.744,0.635,respectively),and the AUC of SS and PLT had statistically difference (P=0.002).In diagnois of moderate or severe GEV,the AUC of MR VS was higher than that of SS,HS and PLT (AUC=0.893,0.816,0.713,0.665,respectively),and statistical differences of AUC were found between MR VS and HS,as well as between MR VS and PLT (P=0.018,0.002).Sensitivities of MR VS combined with SS in differential diagnosis of liver cirrhosis with or without GEV,liver cirrhosis with moderate or severe GEV were 94.16 % and 96.83 %,respectively.Conclusion MRE is effective in the prediction of GEV with severity and diagnostic value equivalent to DCE-MR.

10.
The Journal of Practical Medicine ; (24): 641-644,652, 2018.
Article in Chinese | WPRIM | ID: wpr-697673

ABSTRACT

Objective To explore the diagnostic values of procalcitonin(PCT),C-reactive protein (CRP),white blood cell(WBC),and neutrophil(N%)in bacterial infectious diseases. Methods Selected 272 patients with bacterial infectious diseases as the research object,all the patients were in accordance with diagnostic criteria of infection,and confirmed by laboratory examination and microbial cultures. Results The total sensitivity of bacterial infection was PCT > CRP > N% > WBC,and the specificity was PCT > CRP > WBC > N%. After different bacterial infection,klebsiella pneumonia was larger in view of PCT sensitivity than escherichia coli, followed by staphylococcus aureus,streptococcus pneumonia,and bauman acinetobacter in ranking. In view of CRP sensitivity,streptococcus pneumonia was larger than klebsiella pneumonia,followed by escherichia coli, staphylococcus aureus,and bauman acinetobacter in ranking. In respect of WBC sensitiveness,bauman acineto-bacter was larger than escherichia coli,followed by staphylococcus aureus,streptococcus pneumonia and klebsiella pneumoniae.In respect of N% sensitivity,streptococcus pneumonia was larger than klebsiella pneumonia,followed by bowman acinetobacter,escherichia coli,and staphylococcus aureus in ranking.After bacterial infection,PCT, CRP,WBC and N% were significantly higher than normal control group,and significantly higher than normal refer-ence value. After different bacterial infections,in view of the PCT level,klebsiella pneumonia was higher than streptococcus pneumonia,followed byescherichia coli,staphylococcus aureus,and bauman acinetobacter in rank-ing.In view of CRP level,klebsiella pneumonia was higher than streptococcus pneumonia,followed by escherichia coli,staphylococcal aureus and bowman acinetobacter. In view of WBC level,staphylococcus aureus was higher than bowman acinetobacter,followed by escherichia coli,klebsiella pneumonia and streptococcus pneumoniae. In view of N% level,klebsiella pneumonia was higher than streptococcus pneumonia,followed by bowman acineto-bacter,staphylococcus aureus and escherichia coli. Conclusion In terms of the sensitivity of PCT,CRP,WBC and NEC% to bacterial infection,the sequence is PCT>CRP>N%>WBC.

11.
Article in Chinese | WPRIM | ID: wpr-697083

ABSTRACT

Objective To investigate the influence of WeChat + family centered health intervention on the self-efficacy of elderly hypertensive patients in General clinic. Methods A total of 102 elderly hypertensive patients aged≥60 years in General clinic from February 2017 to July 2017 were enrolled.They were divided into experimental group and control group with the table of random number, with 51 cases in each group. The experimental group used the WeChat+ family centered health intervention model,while the control group adopted the traditional health education model.The changes of blood pressure and self-efficacy were evaluated before intervention, 3 months and 6 months after intervention respectively. Results There were no statistic significant difference in blood pressure and self-efficacy total score between the two groups before intervention(P>0.05). At 3 months after intervention,the blood pressure(systolic pressure/diastolic pressure)of the patients were(144.20±4.60), (80.00±5.00)mmHg(1 mmHg=0.133 kPa)in the experimental group,those of which were(154.20±7.16), (87.00±3.81)mmHg in the control group,the differences were statistically significant (t=-2.628,-2.490,P<0.05).At 6 months after intervention,the blood pressure(systolic pressure/diastolic pressure)of the patients were(141.60±6.43),(76.00±4.85)mmHg in the experimental group,those of which were(151.60± 5.94),(85.40±4.56)mmHg in the control group,the differences were statistically significant (t=-2.555,-3.158,P<0.05).There were differences in systolic and diastolic pressure in patients with different groups at different time,and the differences were statistically significant(F=18.668,18.174,P<0.01).The total score of self-efficacy at 3 months and 6 months after intervention was(30.14±0.43),(32.56±0.23)points in the experimental group and (28.14 ± 0.15), (29.40 ± 0.19) points in the control group, the difference was statistically significant(t=9.736, 23.819, P<0.05). The differences in the total score of patients′self-efficacy in different groups at different time had statistically significance (F=2 631.551, P<0.01). Conclusions The WeChat+family centered health intervention model can significantly improve the self-efficacy of the elderly patients with high blood pressure in the General clinic.

12.
Chongqing Medicine ; (36): 1712-1715, 2018.
Article in Chinese | WPRIM | ID: wpr-692005

ABSTRACT

Objective To investigate the expression changes of p53 and p21 in premature ovarian failure in female rats exposed to coal burning fluorosis.Methods Ablactation 24 SD female rats were chosen to establish animal model (the model group),in fluorine 90 days were put to death.Observed the changes of the teeth of the female rats during fluorine exposure and ovarian granulosa cell premature aging change,detected fluorine contents of urine and bone,and expression of the ovarian granular cell gene p53 and p21.Results In the model group,fluorine spot tooth,the fluorine contents of urine and bone increased significantly.In the model group,there was no premature aging of ovarian granulosa cells in the low fluorine group,but with the increase of dye fluorine metering,ovarian granulosa cells became mild,moderate to severe progressive edema trends,cells morphological damaged fuzzy,and atresia follicles increased significantly,corpus luteum degraded,mature follicle significantly reduced,and the ovarian function gradually signs of premature aging presented.With the increase of dye fluoride measurement,the expression of aging gene p53 and p21 in each group gradually increased,compared with the control group,there was statistically significant difference (P<0.05).Conclusion Premature ovarian failure caused by coal-burning fluorosis was significantly related to the expression of genes p53 and p21.

13.
Chinese Critical Care Medicine ; (12): 266-269,274, 2018.
Article in Chinese | WPRIM | ID: wpr-703636

ABSTRACT

Objective To analyze the clinical manifestation and therapeutic method in patients with acute mushroom poisoning. Methods A retrospective study was conducted. The clinical data of 48 patients with acute mushroom poisoning admitted to Department of Poisoning Treatment of the 307th Hospital of PLA from January 2016 to May 2017 were analyzed. The clinical data including gender, age, clinical symptoms, onset season, initial symptoms, incubation time, the length of hospital stay, treatment, and prognosis. In addition to the conventional treatment, the patients with severe liver damage were treated with continuous blood purification (CBP). The changes in routine blood test, biochemical parameters, blood ammonia and coagulation function before and 1, 3 and 7 days after CBP were observed. Results There were 29 of male (60.4%) and 19 of female (39.6%) in 48 patients with acute mushroom poisoning, with an average age of (48.10±13.14) years. There were 9 patients suffering from gastroenteritis type, 26 suffering from liver damage type, 8 suffering from neuro-psychosis type, 2 suffering from hemolytic type, and 3 suffering from renal damage type. All of the poisoned patients had evident seasonal characteristic, mainly concentrated in the autumn, especially in August, according for 66.7% (32/48). The initial symptoms of poisoning patients were mainly manifested as nausea and vomiting (50.0%). In five kinds of poisoned patients, the incubation time [(1.44±1.15) hours] and the length of hospital stay [(3.50±2.33) days] of neuro-psychosis type was the shortest, and the incubation time of liver-damaged type [(10.63±3.50) hours] and the length of hospital stay of renal damage type [(20.67±0.58) days] was the longest. Patients received symptomatic treatment according to different types, among whom 12 patients with severe liver damage received additional treatment for CBP. After the treatment, alanine aminotransferase (ALT), aspartate aminotransferase (AST), MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH), and prothrombin activity (PTA) were significantly improved as compared with those before CBP treatment, with significant differences between 7 days after CBP and before CBP [ALT (U/L): 213.08±127.30 vs. 2 766.83±1 909.66, AST (U/L): 50.00 (41.00, 85.00) vs. 2 142.00 (1 225.00, 3 126.00), CK-MB (U/L): 24.09±8.87 vs. 44.75±22.09, LDH (μmol·s-1·L-1):3.70±1.46 vs. 13.03±12.77, PTA: (79.08±24.29)% vs. (35.25±19.85)%, all P < 0.01]. Among 48 patients, 47 were cured and discharged, and 1 patient with liver failure died due to aggravation of liver dysfunction, abnormal coagulation and bleeding, and massive hemorrhage of gastrointestinal tract. Conclusions Acute mushroom poisoning patients demonstrated obvious seasonal characteristics, mostly liver-damaged type, and its initial symptoms were mainly presented as nausea, vomiting and other gastrointestinal manifestations. Early clarification of diagnosis, timely treatment, as well as providence with CBP treatment in severe patients should be carried out as soon as possible. In such a way the curative effect can be enhanced, the mortality can be reduced, and the prognosis of the patients could be improved.

14.
Article in Chinese | WPRIM | ID: wpr-701262

ABSTRACT

Objective To investigate the expression of Sirtuin1 (SIRT1) in brain tissue of rats with different doses of fluorosis-induced brain injury.Methods Forty-eight clean SD rats were divided into 4 groups based on body weight (90-100 g) via the random number table method:the control group (normal feed containing fluoride 4.5 mg/kg),low fluoride groups (feed containing fluoride 25.0 mg/kg),middle fluoride groups (feed containing fluoride 50.0 mg/kg),high fluoride groups (feed containing fluoride 100.0 mg&g),twelve rats in each group,half male and half female.Rats in each group drank tap water freely.Low,middle and high fluorine groups were free to eat the designated different formulations of raw coal and mixed peat baked corn feed,other feed ingredients were the same as those in control group,the rats were sacrificed at 90 d of fluorine exposure.At 7 d before the rats were sacrificed,Morris water maze and platform experiment were employed to test the ability of learning and memory in rats.Take the brain tissue and thigh long bones after the rats were sacrificed,immediately.The fluorine ion selective electrode method was used to detect urinary fluoride and fluorine content in bone in rats.The brain SIRT1 mRNA and protein expression levels were detected by Real-Time PCR and Western blotting,respectively.Results In the space exploration experiments,the mean time to first passage of platform of the rats in control group and low,middle and high fluorine groups were (9.8 ± 3.5),(15.8 ± 5.1),(22.2 ± 7.9) and (30.5 ± 8.5) s,respectively.The differences between groups were statistically significant (F =10.853,P < 0.05).The number of passed through the platform of the rats in control and low,middle and high fluorine groups were (5.2 ± 2.1),(3.3 ± 1.6),(1.3 ± 1.1) and (1.2 ± 0.8) time/60 s,respectively.The differences between groups were statistically significant (F =10.105,P< 0.05).In the control group and low,middle and high fluoride groups,the resting time of the original platform quadrant were (30.5 ± 9.8),(22.7 ± 4.6),(13.8 ± 4.8) and (7.0 ± 2.4) s,respectively.The differences between groups were statistically significant (F =17.433,P < 0.05).And the middle,high fluoride groups compared with the control group was significantly different (P < 0.05).With increase of fluoride dosage,the first time to cross the platform gradually extended,the number of crossing the platform and the original platform quadrant dwell time decreased gradually,the differences between the middle,high fluoride groups and the low fluoride group were statistically significant (P < 0.05).The mRNA expression of SIRT1 in control group and low,middle and high fluoride groups were 0.979 ± 0.088,0.907 ± 0.050,0.426 ± 0.073,0.219 ± 0.092,respectively.The differences between groups were statistically significant (F =136.837,P < 0.05).The levels of SIRT1 protein in control group,low,middle and high fluoride groups were 1.224 ± 0.139,0.988 ± 0.096,0.581 ± 0.084 and 0.269 ± 0.066,respectively.The differences between groups were statistically significant (F =107.961,P < 0.05).The levels of SIRT1 mRNA and protein were gradually decreased with increase of fluoride dose in the low,middle and high fluoride groups (P < 0.05).Conclusions Fluorosis can affect the learning and memory ability of rats.SIRT1 mRNA and protein expressed in rat brain is decreased,which is more obvious with the increase of fluoride dose.

15.
Article in Chinese | WPRIM | ID: wpr-666149

ABSTRACT

As a key protein,annexin A5 involves in the process of invasion,metastasis and immune tolerance of tumors.And annexin A5 can not only promote the tumor,but also inhibit it.It has been widely concerned in the basic research targeting annexin AS.Because of the high affinity of annexin A5 for phosphatidylserine,annexin A5 is expected to be a new diagnostic marker and anti-tumor target.

16.
Chinese Pharmacological Bulletin ; (12): 900-904, 2017.
Article in Chinese | WPRIM | ID: wpr-620136

ABSTRACT

Cross-talk of intracellular signaling pathways that share common components (hubs) is organized in form of a bow-tie network topology.Signaling cross-talk is functionally pleiotropic for target genes regulation, resulting in functional redundancy, synergism and antagonism, which should be precisely controlled to prevent signaling 'leaking' or 'spillover'.Thus, the biological system has evolved multiple insulating mechanisms to achieve stimulus-specific response that maintains intracellular homeostasis.The insulation mechanism of signaling cross-talk suggests: (1) the functional duality of cross-talk molecules that determine cell fate requires selectively targeting dysregulated cross-talk molecules while protecting the normal ones from off-target or unintended effects, and we propose them as the targetable cross-talk molecules;(2) cross-talk molecules are usually carried on the macromolecular complex as their functional platforms, thus the structural plasticity of conformational changes at the interaction surface of cross-talk molecules asks for intensive work on the relationship study between drug binding and biological activity, which we propose as the accessible cross-talk molecules.Therefore, signaling cross-talk and its insulation mechanism play instructive leading roles in resolving the bottlenecks of current drug R&D and improve the clinical outcome.

17.
Chinese Journal of Radiology ; (12): 366-371, 2017.
Article in Chinese | WPRIM | ID: wpr-513023

ABSTRACT

Objective To investigate the feasibility of spin-echo echo-planar imaging(SE-EPI) of magnetic resonance elastography (MRE) for the diagnosis of pancreatic mass and to evaluate the difference of stiffness value in different pancreatic mass. Methods In a retrospective study, MRE was performed in 20 healthy volunteer (control group) and 62 patients with surgery-proved pancreatic masses, including pancreatic cystadenoma in 5 cases and solid masses of pancreas in 57 cases (39 with malignant mass and 18 with benign mass) using 3.0 T MRE. The differences of stiffness between two groups were evaluated using non-parametric Mann-Whitney U test. ROC was used to assess the diagnostic ability of elastogram for the detection of pancreatic masses, and to evaluate MRE-determined stiffness for the differentiation of masses from healthy pancreas, and malignancy from benign solid masses. Results The total detection rate of elastogram was 91.9%(57/62),with false positive rate of 5.0%(1/20) and false negative rate of 8.1%(5/62). The median stiffness value in the control group vs patient group were 1.18 kPa (interquantile range:1.15 to 1.30) kPa and 2.43 kPa (interquantile range:1.94 to 3.64 kPa) respectively, with statistically significant differences (Z=-5.967,P<0.01). The sensitivity and specificity of stiffness (≥1.36 kPa) to differentiate pancreatic mass from healthy pancreas was 93.6%and 100.0%, respectively, and to differentiate benign and malignant solid masses (≥2.17 kPa) was 82.1%and 83.3%, respectively. Conclusion MRE is a promising method in detecting and distinguishing different pancreatic masses.

18.
Article in Chinese | WPRIM | ID: wpr-512767

ABSTRACT

Objective:To analyze and summarize the causes of unscheduled suspension of knee and hip arthroplasty and to provide the method for optimizing the patient's pre-operative management and improving the efficiency of medical resources as well as the patient's satisfaction.Methods: The data for this report was retrospectively collected from September 2013 to August 2014 in our hospital,from cases of knee and hip arthroplasty that were suspended before the scheduled operation time.Acquisition data from the collected cases including the patients' gender,age and the surgical procedure.At the same time,the suspension reasons were recorded and analyzed.All the decisions of suspension was made by the surgeons and the anesthesiologists according to the abnormal result of preoperative examinations,after communicating with the patients and their families and obtaining their understandings.Results: In the collecting period,our department scheduled 1 146 cases of knee and hip arthroplasty,among which 1 003 were completed,143 suspended (12.5% suspension rate).Among the causes of suspension,the top four common causes were cardiovascular disease (44/143,31%),other infections (20/143,14%),bacteriuria (18/143,13%) and inappropriate surgical indication (16/143,11%).Other causes include surgeon's reason,Blood system abnormalities,high inflammatory index,deep vein thrombosis,other diseases uncontrolled,abnormal liver function and poor diabetes mellitus control,etc.For the rate of suspension,there was no significant difference between the patients with different genders (male: 15.0%,and female: 11.7%,P=0.149),or age (≤50 years: 13.0%;51-65 years: 11.6%;66-80 years 13.3%;>80 years 11.1%;P=0.864).However compared with knee arthroplasty,hip arthroplasty had a higher suspension rate (knee arthroplasry 11.1%,hip arthroplasry 16.1%,P=0.021).Conclusion: It is important to educate and manage the patients before their knee and hip arthroplasty.Through clear diagnosis,detailed medical history analysis careful physical examination,and targeted outpatient examinations and tests for which priority was focused on cardiovascular or other system diseases we could minimize the occurrence of operative suspension post hospitalization,therefore improving the efficiency of the use of medical resources.

19.
Chongqing Medicine ; (36): 3343-3345, 2017.
Article in Chinese | WPRIM | ID: wpr-614710

ABSTRACT

Objective To observe the short term clinical effect of OSSTEM implant system in the treatment of submerged and non-submerged healing in posterior region.Methods Sixty-two patients(80 OSSTEM implants) in the oral and maxillofacial surgery department of the First Affiliated Hospital of Chongqing Medical University from July 2013 to July 2015 were randomly divided into the group A (submerged healing) and B (non-submerged healing).The lack of teeth area in all subjects was performed the OSSTEM artificial teeth routine implant,moreover the changes of peri-implant bone level,gingival bleeding index and implant retention rate were performed the comparative analysis after 1-year load.The peri-implant bone level was performed the statistical analysis by adopting the independent sample T test and the gingival bleeding index was analyzed by adopting the Fisher exact probability test using SPSS17.0 software package.Results The implant retention rates in both groups were 100%.The medial peri-implant bone levels were (0.59±0.19) mm in the group A and (0.58±0.21)mm in the group B,the difference had no statistical significance(P>0.05).The distal peri-implant bone levels were (0.55±0.19) mm in the group A and (0.56±0.20)mm in the group B,the difference between the two groups had no statistical significance(P>0.05).Conclusion The submerged healing and non-submerged healing in OSSTEM implant system can achieve good implant healing of soft and hard tissue and bone integration,the effect is good,which all can serve as the routine healing mode of OSSTEM implant.

20.
Protein & Cell ; (12): 722-734, 2016.
Article in English | WPRIM | ID: wpr-757383

ABSTRACT

Programmed cell death 4 (PDCD4) is a RNA-binding protein that acts as a tumor suppressor in many cancer types, including colorectal cancer (CRC). During CRC carcinogenesis, PDCD4 protein levels remarkably decrease, but the underlying molecular mechanism for decreased PDCD4 expression is not fully understood. In this study, we performed bioinformatics analysis to identify miRNAs that potentially target PDCD4. We demonstrated miR-181b as a direct regulator of PDCD4. We further showed that activation of IL6/STAT3 signaling pathway increased miR-181b expression and consequently resulted in downregulation of PDCD4 in CRC cells. In addition, we investigated the biological effects of PDCD4 inhibition by miR-181b both in vitro and in vivo and found that miR-181b could promote cell proliferation and migration and suppress apoptosis in CRC cells and accelerate tumor growth in xenograft mice, potentially through targeting PDCD4. Taken together, this study highlights an oncomiR role for miR-181b in regulating PDCD4 in CRC and suggests that miR-181b may be a novel molecular therapeutic target for CRC.


Subject(s)
Animals , Apoptosis Regulatory Proteins , Genetics , Metabolism , Caco-2 Cells , Cell Proliferation , Colorectal Neoplasms , Genetics , Metabolism , Pathology , Heterografts , Humans , Male , Mice , Mice, Nude , Mice, SCID , MicroRNAs , Genetics , Metabolism , Neoplasm Proteins , Genetics , Metabolism , Neoplasm Transplantation , RNA, Neoplasm , Genetics , Metabolism , RNA-Binding Proteins , Genetics , Metabolism
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