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1.
Article in Chinese | WPRIM | ID: wpr-1019223

ABSTRACT

Objective To explore the treatment gap and influencing factors of convulsive epilepsy in rural areas of Jiangsu Province.Methods The clinical data of 7836 rural convulsive epilepsy patients screened from 2005 to 2020 were statistically analyzed,and the treatment status,treatment gap and related influencing factors of epilepsy patients were analyzed.Results A total of 7836 patients with convulsive epilepsy were enrolled in this study.The treatment gap for convulsive epilepsy in rural areas of Jiangsu Province was 69.05%.There was no significant difference in the treatment gap between different genders(P>0.05).There were statistically significant differences in the treatment gap between age(χ2 = 12.196,P =0.007),age of onset(χ2 =58.658,P<0.001),disease duration(χ2 =65.430,P<0.001),seizure frequency(χ2 =171.276,P<0.001),and hospitalization level(χ2 = 122.076,P<0.001).Multivariate Logistic regression analysis showed that the older the age of onset was,the shorter the course of the disease was,the more frequent the seizures was,and the greater the treatment gap in patients with epilepsy was(all P<0.05).Patients aged 45-59 years(P =0.012)and treated in municipal and county hospitals(P<0.001)were more likely to receive regular anti-epileptic treatment.Conclusions There is a significant treatment gap for convulsive epilepsy patients in rural areas of Jiangsu Province.This may be due to insufficient awareness of epilepsy and the underdevelopment of primary healthcare institutions.

2.
Article in Chinese | WPRIM | ID: wpr-1025552

ABSTRACT

Objective:To explore the psychometric characteristics of the Chinese version of the health-related social capital scale.Methods:From March to May 2020, after the original scale was translated into Chinese following the Brislin improved translation model, 251 community-dwelling senior citizens aged 65 and above were selected by convenient sampling method. Data analysis was conducted using SPSS 22.0 and AMOS 22.0, including tests of significance, correlation analysis, exploratory factor analysis, and confirmatory factor analysis.Results:Exploratory factor analysis extracted three factors: social participation, social cohesion, and reciprocity, which accounted for a cumulative contribution rate of 61.72%. Confirmatory factor analysis showed that the three-factor model fitted well(χ 2/ df=1.22, RMSEA=0.04, CFI=0.98, GFI=0.93, IFI=0.98, TLI=0.97). Social capital was significantly correlated with perceived social support positively ( r=0.36, P<0.01), and with loneliness negatively ( r=-0.30, P<0.01). The three factors were significantly correlated with the total scale ( r=0.85, 0.50 and 0.52, respectively, all P<0.01). And correlations between the items of each factor were 0.24-0.55, 0.30-0.59, 0.44-0.70, respectively(all P<0.01). The Cronbach's α coefficients of the total scale and three factors were 0.74, 0.72, 0.65 and 0.62, respectively(all P<0.01), and their retest reliability were 0. 92, 0. 87, 0. 82 and 0. 96, respectively(all P<0.01). Conclusion:The Chinese version of health-related social capital scale conforms to the three-factor model with good reliability and validity, which can be used to assess the social capital status of community-dwelling older adults in China.

3.
Chinese Journal of Neurology ; (12): 1213-1216, 2023.
Article in Chinese | WPRIM | ID: wpr-1029135

ABSTRACT

Epilepsy is a common neurological disease, and studies have shown that some epilepsy patients are combined with cognitive impairment. In clinical practice, it has been found that, in addition to the seizure itself, anti-seizure medications can also have a corresponding impact on the cognitive function of epilepsy patients. However, the effects of some drugs on the cognitive function of epilepsy patients are still inconclusive, and the mechanism of their influence on cognitive function in epilepsy patients is not elucidated. In recent years, some studies followed up the cognitive function of patients receiving anti-seizure medications therapy, and put forward guidance. This article focused on the research of commonly used drugs on cognitive function, the possible mechanism of drugs affecting cognitive function, the rational use of drugs in different groups of people and the prevention strategies of related cognitive dysfunction, hoping to improve emphasis on cognitive function in epilepsy patients and guide clinical practice.

4.
Article in Chinese | WPRIM | ID: wpr-931889

ABSTRACT

Objective:To translate the English version of the health enhancement lifestyle profile-screener into Chinese and assess its reliability and validity based on classical test theory(CTT) and Rasch model among the elderly.Methods:A total of 447 older adults from rural and urban areas in Sichuan Province from December 2020 to March 2021 were selected by convenience sampling, and were surveyed with the revised Chinese version of health enhancement lifestyle profile-screener(HELP-CS). Rasch and CTT-based approaches were used to evaluate the unidimensionality data-model fit, internal consistency, test-retest reliability, criterion validity and discriminant validity of the questionnaire.Results:According to the results of the initial test questionnaire, the ninth question was deleted, and 14 items were retained to form a formal questionnaire. The formal test results showed that HELP-CS met the unidimensionality, and the residual mean square error for item 2 and 14 were within the acceptable range, which were 1.49 and 1.41. Item 10 and 12 had the highest logits, which were 1.87 and 1.91 respectively. There were gender differences in some topics. The Cronbach′s α of the scale was 0.719, and the test-retest reliability was 0.704 after two months. The criterion correlations with HPLP-CE were 0.581.Conclusion:HELP-CS has good adaptability, reliability and validity in the elderly, and can be used as a quick and effective screening tool in the study of the lifestyle of the elderly.

5.
Article in Chinese | WPRIM | ID: wpr-867187

ABSTRACT

Objective:To investigate the mediating and moderating effects of resilience on frailty and depression in the community-dwelling old adults.Methods:Totally 871 community-dwelling old adults chosen from different communities were investigated by the geriatric depression scale, Tilburg frailty indicator and 10-item Connor-Davidson resilience scale.SPSS 22.0 and AMOS 24.0 were used for data analysis, including descriptive analysis, correlation analysis, structural equation modeling and hierarchical regression analysis.Results:(1)The score of frailty (3.72±2.89)was positively correlated with the score of depression (2.63±2.57, r=0.16-0.58, P<0.01). The score of resilience(28.24±6.80) was negatively correlated with the score of frailty and depression ( r=-0.10~-0.49, both P<0.01), and frailty predicted 35% of the total variation of depression in the elderly.(2)The mediating effect of resilience was significant and the indirect effect was 0.10, accounting for 12.66% of the total variance.(3) Resilience moderated the relationship between frailty and depression ( β=-0.12, t=-4.11, R2=0.41, P<0.001). The frailty of old adults with lower resilience played a stronger predictive role in depression (simple slope=0.50, t=14.73, R2=0.01, P<0.001). Conclusion:There is a close relationship between frailty and depression in the community-dwelling older adults, and resilience plays both mediating and moderating role in the relationship.

6.
Article in Chinese | WPRIM | ID: wpr-744782

ABSTRACT

Objective To investigate the resilience characteristics of the elderly disabled,and the effects of individual,family and social factors on resilience.Methods A total of 138 disabled older adults from four districts of Chengdu City were investigated by 10 items Connor-Davidson resilience scale (CDRISC-10),the automatic thoughts questionnaire (ATQ),self esteem scale (SES),revised eysenck personnality questionnaire short scale for chinese(EPQ-RSC) and self-made population sociology questionnaire.Totally 194 community-dwelling older adults without disabilities,as a comparative population,were included in the analysis.Results Compared with the community-dwelling elderly without disability (35.58 ± 5.75,18.13±3.48,18.43±3.08),the scores of resilience(21.38±7.71),strength(10.50±4.08) and hardiness (10.88±4.07) of the elderly disabled were significantly lower(t=-19.56,-17.81,-18.34,P<0.01).There were no significant differences in the resilience scores of the elderly disabled in terms of gender,urban and rural sources,marital status,education,disability level and family atmosphere.However,there were significant differences in age,disability causes,disability type and family economic conditions (age:thardiess =2.18,P<0.05.disability causes:tresilience =-2.75,tstrength =-2.22,thardiness =-2.68,P<0.05.disability type:Fhardiness =2.60,P< 0.05.family economic conditions:Fresilience =5.45,Fstrength =4.78,Fhardiness =5.21,P<0.01).The resilience,automatic thoughts,self-esteem and personality were significant correlation with each other(r=-0.16-0.65,P<0.05).After controlling age,disability causes,disability type and family economic conditions,self esteem(β=0.291,P<0.01),extraversion (β=0.287,P<0.01) and neuroticism (β=-0.254,P<0.05) were important influential factors of resilience,explained 32.0% variance in resilience.Conclusion It may be an important way to improve resilience level of the elderly disabled by enhancing their selfesteem,promoting their psychological adjustment and strengthening their emotional management.

7.
Article in Chinese | WPRIM | ID: wpr-801383

ABSTRACT

Objective@#To explore the current status and predictive factors of frailty among community-dwelling old adults.@*Methods@#A total of 1 012 community-dwelling older adults aged 65 years and above in Sichuan Province were enrolled and investigated by self-made general condition scale, Tilburg Frailty Indicator (TFI), Short Physical Performance Battery(SPPB), 10-item Connor-Davidson Resilience Scale(CD-RISC-10), Mini-Mental State Examination(MMSE) and Geriatric Depression Scale Short-form(GDS-15).@*Results@#(1) The prevalence of frailty was 30.73%.(2) There were significant differences in the frailty scores of community-dwelling older adults in terms of gender, education, marital status, living condition, spouse support, children support, family economic conditions, self-perceived health status, number of chronic diseases, body mass index, number of falls in the past year, exercise state and sleep time at night(all P<0.05). (3) The frailty (3.72±2.88) was significantly positively correlated with depression (2.69±2.58; r=0.58, P<0.01), and negatively correlated with the scores of balance ability(3.28±1.01), walking speed(2.92±1.05), chair rise test(2.92±1.27), strength of resilience(13.99±3.57) and MMSE(23.71±5.55) (r=-0.33-0.43, P<0.01). (4) Depression (β=0.416, P<0.001), the number of chronic diseases(β=0.219, P<0.001), balance ability(β=-0.166, P<0.001), spousal support(β=-0.089, P<0.01), strength of resilience(β =-0.097, P<0.01), family economic status(β=0.093, P<0.01) and gender(β=0.082, P<0.01) significantly influenced frailty, with a combined explanation variance of 45.9%.@*Conclusions@#The rate of frailty of community-dwelling old adults is high, which is influenced by physiological, psychological and social factors.Therefore, it is necessary to adopt a comprehensive model of multi-disciplinary cooperation to carry out frailty intervention in community.

8.
Article in Chinese | WPRIM | ID: wpr-824259

ABSTRACT

Objective To explore the current status and predictive factors of frailty among community-dwelling old adults.Methods A total of 1 012 community-dwelling older adults aged 65 years and above in Sichuan Province were enrolled and investigated by self-made general condition scale,Tilburg Frailty Indicator (TFI),Short Physical Performance Battery (SPPB),10-item Connor-Davidson Resilience Scale (CD-RISC-10),Mini-Mental State Examination (MMSE) and Geriatric Depression Scale Short-form (GDS-15).Results (1) The prevalence of frailty was 30.73%.(2) There were significant differences in the frailty scores of community-dwelling older adults in terms of gender,education,marital status,living condition,spouse support,children support,family economic conditions,self-perceived health status,number of chronic diseases,body mass index,number of falls in the past year,exercise state and sleep time at night(all P<0.05).(3) The frailty (3.72±2.88) was significantly positively correlated with depression (2.69± 2.58;r=0.58,P<0.01),and negatively correlated with the scores of balance ability(3.28± 1.01),walking speed (2.92± 1.05),chair rise test (2.92± 1.27),strength of resilience (13.99±3.57) and MMSE (23.71 ±5.55) (r=-0.33-0.43,P<0.01).(4) Depression (β=0.416,P<0.001),the number of chronic diseases (β=0.219,P<0.001),balance ability (β=-0.166,P<0.001),spousal support (β=-0.089,P<0.01),strength of resilience(β =-0.097,P<0.01),family economic status(β=0.093,P<0.01) and gender(β=0.082,P<0.01) significantly influenced frailty,with a combined explanation variance of 45.9%.Conclusions The rate of frailty of community-dwelling old adults is high,which is influenced by physiological,psychological and social factors.Therefore,it is necessary to adopt a comprehensive model of multi-disciplinary cooperation to carry out frailty intervention in community.

9.
Article in Chinese | WPRIM | ID: wpr-704136

ABSTRACT

Objective To explore the association between the ZNF804A gene genetic variation poly-morphism rs1344706 and brain structure and function in patients with schizophrenia. Methods Literature search was conducted in Pubmed and other databases,the processes were performed in strict accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines,then the Comprehensive Meta-Analysis software was used for meta-analysis. Results Schizophrenia patients with rs1344706 risk al-lele had lager gray matter in the amount of brain regions including frontal lobe (z=3.445,P=0.001),tempo-ral lobe (z=2.140,P=0.032) and other brain regions; healthy controls with the risk allele had smaller gray matter and regional activity in the frontal lobe ( gray matter: z=-2.008, P=0.045, regional activity: z=-4.036,P<0.01) and other regions. Sensitivity analysis was stable,but publication bias existed in a few ana-lyses of indexes. Conclusion The risk allele in ZNF804A gene rs1344706 has positive effects on the brain structure in patients with schizophrenia,but negative effects on the brain structure and function in the healthy individuals.

10.
Article in Chinese | WPRIM | ID: wpr-704189

ABSTRACT

Objective To evaluate the reliability and validity of 10 items Connor-Davidson resili-ence scale ( CD-RISC-10 ) in the community-dwelling older adults. Methods Totally 620 community-dwelling older adults randomly chosen from 16 communities in Chengdu city were investigated by CD-RISC-10,the geriatric depression scale short form ( GDS-SF) and self-made basic condition questionnaire. Results The discrimination of the 10 items was statistically significant( t=6. 84-13. 57,P<0. 01) . Exploratory factor analysis showed two factors ( strength and hardiness) were extracted,and the cumulative variance interpreta-tion rate was 42. 32%. Confirmatory factor analysis showed that model index of two sub-factors (χ2/df=1. 518( P<0. 001) ,CFI =0. 964,TLI =0. 948,IFI =0. 965,NFI =0. 904,RMSEA=0. 042) . Cronbach's αof the total scale and the two sub-factors were 0. 737,0. 673 and 0. 585 respectively. After half a month,the retest reliability coefficients of the total scale and the two sub-factors were 0. 974,0. 932 and 0. 941(P<0. 01) . Conclusion The CD-RISC-10 scale has acceptable reliability and validity,with using easily and con-veniently. Therefore,it is an effective tool for measuring the resilience of the community-dwelling older adults.

11.
Chinese Journal of Pathophysiology ; (12): 1520-1523, 2017.
Article in Chinese | WPRIM | ID: wpr-608872

ABSTRACT

AIM: To evaluate the effect of GYY4137, a novel hydrogen sulfide (H2S) donor, on cytosolic lipid decomposition in mouse primary steatosis hepatocytes.METHODS: Oleic acid (OA) was used to induce hepatic steatosis model in vitro.The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion were divided into 4 groups: the cells in control group were incubated with normal medium for 54 h;the cells in model group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 for 6 h;the cells in H2S group or DL-propar-gylglycine (PAG;an inhibitor of cystathione γ-lysase, inhibiting H2S synthesis) group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 which contained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h.The glycerin release and the protein expression of hormone-sensitive lipase (HSL) in the cells were mea-sured.RESULTS: Compared with model group, the glycerin release and the protein expression of phosphorylated HSL (p-HSL) in H2S group decreased significantly, while those increased significantly in PAG group.CONCLUSION: In steatosis hepatocytes, exogenous H2S possibly decreases cytosolic lipid decomposition by decreasing the protein level of p-HSL.

12.
Article in Chinese | WPRIM | ID: wpr-511056

ABSTRACT

Objective To explore the characteristics of approach and avoidance behaviors among subthreshold depressed adolescents. Methods 27 subthreshold depressed adolescents and 29 healthy con?trol teenagers completed the approach?avoidance task,and the reaction time at approach and avoidance task of the two groups was compared. At the same time,their differences were analyzed between the two groups on the Behavioral Inhibition/Activation System Scale scores. Results ( 1 ) Subthreshold depression group (1208.18±338.92)ms) had significantly longer reaction time than those in healthy control group((1058.40 ±204.22)ms) when approaching happy expression ( t=2.02, P<0.05). (2) Subthreshold depression group (37.33±4.46) had significantly lower behavioral activation system scale scores than those in healthy control group ((39.76±3.75), t=-2.21, P<0.05); and the score of reward responsiveness was marginally signifi?cant lower in subthreshold depression group (12.63±1.67) than that in healthy control group ((13.41± 1.30), t=-1.97, P=0.054). Conclusion To activate the approach behaviors of subthreshold depression adolescents may be helpful for reducing the symptoms of depression.

13.
Article in Chinese | WPRIM | ID: wpr-616220

ABSTRACT

Objective To investigate the role of resilience on cognitive function in older adults.MethodsBy random sampling,a total of 321 older adults participated the present study and were investigated by Connor-Davidson Resilience Scale,Mini Mental State Examination and self-designed general questionnaire.ResultsThe score of resilience in older adults (82.75±15.13) was significantly higher than that of Chinese community people (65.40±13.90) (t=20.68,P<0.01).Older adults with poor resilience performed significantly lower on all dimensions of cognitive abilities than the other two groups (middle & higher resilience group) (all P<0.05).With both age and educational level controlled,tenacity and strength were of positive prediction for the cognitive abilities of older adults (β=0.25,0.17,P<0.05).ConclusionThere is a close relationship between resilience and cognitive function of older adults.The training of tenacity,strength and optimism will help delay the decline of cognitive function.

14.
Article in Chinese | WPRIM | ID: wpr-488141

ABSTRACT

Objective To examine the amplitude differences of feedback related negativity ( FRN) and P300 among people with different levels of behavioral inhibition system(BIS)/behavioral activation sys-tem( BAS) in feedback stage in a conflict experimental paradigm.Methods According to Behavioral Inhibi-tion/Activation System Scale,the undergraduates were divided into high score or low score groups of BIS and BAS.Using event related potential (ERP) method,feedback related negativity (FRN) and P300 were col-lected and analyzed when they performed a conflict experimental paradigm which had correct or wrong feed-back.Results (1)The amplitudes of FRN(FRN for error feedback:(6.61±1.02)μV,FRN for correct feedback:(7.52±1.15)μV, F(1,20)=5.73, P=0.027)and P300(P300 for error feedback:(14.24±1.09)μV, P300 for correct feedback:(11.58±1.21)μV, F(1,20)=16.72, P=0.001) in BAS high and low score groups were significantly higher in error feedback.( 2) The amplitudes of FRN in BIS high and low score groups were significantly higher in error feedback(FRN for error feedback:(6.63±1.22)μV,FRN for correct feed-back:(8.22±1.24)μV, F(1,22)=17.35, P<0.01).(3) The amplitude of P300 in BIS low score group was higher than that in BIS high score group (BIS low score group:(15.66±1.13)μV,BIS high score group:(11.10±1.04)μV, F(1,22)=17.35, P<0.01).Conclusion BIS and BAS not only relate to approach and a-voidance motivation,but also closely relate to cognitive regulation.

15.
Article in Chinese | WPRIM | ID: wpr-469404

ABSTRACT

Objective To develop social integration scale of the disabled,and to test the reliability and validity of the scale.Methods 511 disabled persons were investigated by self-made social integration scale and Chinese version of positive and negative affect schedule (PANAS-C).Results The Scale was composed of three factors:self integration,relationship integration and affection integration,and they explained 47.39% of variance.The theoretical model fit could be accepted (x2/df =2.051,P<0.001;RMSEA =0.046;GFI =0.884;CFI =0.926;IFI =0.927).The factors had significant correlated with positive affect (r=0.34-0.55,P<0.01)and positive affect (r=-0.12--0.32,P<0.01).The high score group and low score group of social integration had significant differences in positive affect (t=13.77,P<0.01)and negative affect(t=-7.58,P<0.01).The cronbach's alpha coefficients of the scale and the three factors were 0.94,0.92,0.87 and 0.89 respectively,and the seven sub-factors'cronbach 's alpha coefficients were from 0.76 to 0.87.Conclusion The theory construct of social integration of the disabled is multi-hierarchical and multi-dimensional.The scale has acceptable reliability and validity,and can be used to assess the degree of social integration of the disabled.

16.
Article in Chinese | WPRIM | ID: wpr-488383

ABSTRACT

Objective To explore the relationship between resilience and personality among the physically disabled people.Methods A total of 668 disabled persons were tested by Connor-Davidson Resilience Scale(CD-RISC), Eysenck Personality Questionnaire Short Scale (EPQ-RSC), Rosenberg Self-Esteem Scale (SES).Results The score of resilience of disabled people(53.42± 18.18) was than lower Chinese community people (65.4 ± 13.9, t =-16.83, P< 0.05).The scores of the three sub-factors were tenacity (27.19 ± 10.23), strength (18.11 ±6.42) ,optimism(8.09±3.00).The disabled persons' resilience was positively correlated with self-esteem(r=0.52,P<0.01) ,extraversion(r=0.47, P<0.01) ,and negatively correlated with psychoticism(r=-0.34, P<0.01), neuroticism(r=-0.32, P<0.01).Self-esteem and extraversion could make positive predictions,while psychoticism and neuroticism could make negative predictions to the resilience of disabled persons.Conclusion The disabled Persons' personality is closely related with resilience and has significant predictions to resilience.

17.
Article in Chinese | WPRIM | ID: wpr-480304

ABSTRACT

Objective To explore the characteristics of resilience and the relationships between resilience and social integration of disabled people.Methods By convenient sampling,a total of 702 disabled people were investigated by Connor-Davidson Resilience Scale,Social Integration Scale of the Disabled and self-made general questionnaire.Results The score of resilience of disabled people(53.27± 18.21) was lower than chinese community people(65.4± 13.9,t=-13.19,P<0.01).The resilience scores were significantly lower of the disabled people who came from rural areas,younger(≤ 24 years old) or older(≥ 60 years old),and were disabled from birth had poor family economic conditions,and had no insurances(P<0.05,P<0.01).But the disabled people who had university degree or above,married,working,and had warm and harmonious family atmosphere,had significantly higher resilience scores (P<0.05,P<0.01).The vesilience and three sub-factors were significantly correlated with social integration (r=0.38-0.66,P<0.01).The high resilience group had higher score in integration and three dimensions compared with the low resilience group(t=11.60-19.02,P<0.01).Tenacity and strength directly influenced the three dimensions of social integration (the path coefficient were 0.18-0.46,P<0.01).Conclusion The resilience of the disabled people is lower,and there are close relationships between resilience and social integration.The training of tenacity and strength is beneficial to social integration of the disabled people.

18.
Acta Pharmaceutica Sinica ; (12): 1000-7, 2015.
Article in Chinese | WPRIM | ID: wpr-483408

ABSTRACT

Recent data have revealed that inhibiting autophagy exacerbates lipid accumulation in hepatocytes and nitrite treatment reduces total triglyceride levels in the high-fat diet mice. Therefore, the present study aimed to determine the effects of nitrite on simple hepatic steatosis and the possible role of autophagy. Firstly, steatotic L-02 cells were induced by incubating L-02 cells with 1.2 mmol · L(-1) oleic acid (OA) for 24 h. Secondly, steatotic L-02 cells were treated with 0.2 mmol · L(-1) sodium nitrite (SN) plus 3-methyladenine (3-MA), or chloroquine (CQ) for 24 h, and then lipid accumulation was measured with oil red O staining and triglyceride quantification. The notable steatosis could be observed in L-02 cells following exposure to 1.2 mmol · L(-1) OA for 24 h. Treatment with 0.2 mmol · L(-1) sodium nitrite reduced lipid accumulation in steatotic L-02 cells. 3-MA weakened the ability of sodium nitrite to ameliorate hepatic steatosis. Additionally, the sodium nitrite increased number of LC3-II immunostaining puncta and LC3-II protein expression was confirmed by immunofluorescence or Western blot analysis, and the effects were enhanced by CQ treatment. The number of increased cytoplasm vacuoles and lysosomes increased was confirmed by phase contrast and fluorescence microscope respectively. The increased autolysosome was detected by electron microscopy, this phenomenon could be reversed by CQ treatment. These data demonstrated that sodium nitrite enhanced the autophagic flux and decomposition of triglycerides in steatotic L-02 cells.

19.
Chinese Pharmacological Bulletin ; (12): 945-950,951, 2015.
Article in Chinese | WPRIM | ID: wpr-600618

ABSTRACT

Aim To investigate the effect of hydrogen sulfide on hepatic lipid accumulation in obese mice. Methods C57 BL/6 J mice were randomly divided into control group, model group, and NaHS group. The mice of the control group were fed with normal diet. The mice of the model group and the NaHS group were fed with high-fat diet. From the thirteenth week, the mice of NaHS group were injected intraperitoneally with NaHS (H2S donor) in a dose of 50 μmol·kg-1 per day for 4 weeks and the mice of the model group were injected with the same volume of saline. All mice were sacrificed at the end of the 16th week. The tis-sues of liver were homogenized and centrifugated. The supernatants were used for the determination of triglyc-eride and cholesterol in liver. The morphology of liver was tested by H&E staining. Liver lipid accumulation was determined by oil red staining. Total RNA was ex-tracted from frozen tissue of liver. PCR was used to de-tect CPT-1 , FAS gene expression and ELISA method was used to detect CPT-1,FAS activity in mice liver. Results The body weight of the mice from NaHS group and model group was bigger than that of the mice from control group. Compared with the model group, the body weight of the mice from NaHS group was less;the content of triglyceride and cholesterol in liver was lower; the degree of liver tissue pathological changes and lipid accumulation were alleviated; CPT-1 expres-sion and activity were increased; FAS expression and activity were decreased. Conclusions These data in-dicate that hydrogen sulfide can reduce the lipid con-tent of liver tissue in obese mice and alleviate fatty liv-er. The mechanism may be associated with the in-creased expression of CPT-1 and the decreased expres-sion of FAS in liver.

20.
Article in Chinese | WPRIM | ID: wpr-470637

ABSTRACT

Objective To explore the relationship between subjective well-being (SWB) and behavioral inhibition/activation system (BIS/BAS) of older adults.Methods Using cluster sampling method and convenient random sampling method respectively for older adults in the nursing home and living home,we got 185 older subjects; then using BIS/BAS scale and SWB scale to test 185 older adults by oral questionnaire interviews face to face.Results (1) The SWB average score was 27.39±10.96,the BIS average score was 11.29±4.30,the BAS average score was 32.96±9.13.The SWB of older adults had a significant difference between older adults in the nursing (29.81±9.91) and living home (25.09± 11.47) (t=-2.69,P<0.01).The negative experience of SWB had significant differences between older adults in the nursing (4.25±3.37) and living home (4.25±3.37) (t=3.58,P<0.01),and also between males (4.25±3.37) and females (5.91±3.83) (t=-1.96,P<0.05).The BAS fun seeking had a significant gender difference (t=2.05,P<0.05),which was higher in males (12.60±3.48) than in females (11.40±3.67).(2) BAS was positively correlated with SWB (r=0.40,P<0.01),and BIS was negatively correlated with SWB (r=-0.40,P<0.01).(3) The BAS drive could forecast the 19% variance of SWB.Conclusion BIS/BAS are correlated with SWB for older adults and the BAS drive is one of factors contributing to the SWB of older adults.

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