ABSTRACT
Objective To optimize the formulation and preparation technology of docetaxel(DTX)-loaded nanobubbles,and evaluate their properties. Methods The best ratio of methoxypoly(ethylene glycol)-poly(DL-lactide-co-glycolide)(mPEG-PLGA) and different membrane stabilizers were optimized with Langmuir membrane balance. DTX-Loaded nano-micelles were encapsulated by mPEG-PLGA with an injection method. Nanoemulsions was formed after being added with perfluoropentane(PFP,the boiling point of 29.5℃)as the organic phase. The formulations and preparation techniques of nanoemulsions were optimized and the conditions of nanobubbles formulaion was also evaluated. Cytotoxicity of DTX-loaded nanobubbles on MCF-7 cells was evaluated with the MTT meth?od. Results The best ratio of mPEG-PLGA and Span 20 was 10∶1(mol/mol). The membrane elasticity of the prepared nanobubbles with optimized formulations and the preparation techniques was good. In addition,they were obviously temperature-sensitive and ultra?sonic-sensitive. The particle size of nanobubbles increased with higher temperature and decreased with lower temperature. The size of nanobubbles increased upon ultrasound application and then decreased a little. The cytotoxicity of DTX-loaded nanobubbles on MCF-7 cells was obvious and dose-dependent. Conclusion DTX-Loaded nanobubbles are a novel formulation with tumor-targeted and ultra?sound-sensitive drug release.