ABSTRACT
OBJECTIVE:To determine the contents of secnidazole in secnidazol e tablets by RP-HPLC.METHODS:The chromatographic column was VP-ODS C 18 with acetonirile-0.05mol/L potassium dihydrogen phosphate-triethylamine(15∶85∶0.4)as the mobile phase,the detection wavelength was311nm and the flow rate was0.8ml/min.RESULTS:There was a good linear relationship when the sample size was within the range of0.166?g～0.832?g(r=0.9999),the average re?covery was99.84%(RSD=1.05%,n=15).CONCLUSION:The method is simple and accurate,sensible and reproducible,which can be used for the assaying of both the raw materials and preparations and the quality control of secnidazole tablets.
ABSTRACT
AIM: To determine the content of terpenelactones in Yinxingye Soft Capsule(Folium Ginkgo). METHODS: HPLC with Evaporative Light Scattering Detector (ELSD) was established to determine the content of terpenelactones in Yinxingye Soft Capsule. The analysis was carried out on C 18 column. The mobile phase was 1-propanol-THF-water(1∶15∶84). The operating conditions of the ELSD were a nebulizer nitrogen flow-rate of 3.5L?min -1 and an evaporator tube temperature of 115℃. RESULTS: No interference was found in the terpenelactones peak position. The method was simple, with a good linear relationship. The relative standard deviation of reproducibility was 1.04% and the average recovery was 97.1%. CONCLUSION: The method can be used for quality control.