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1.
China Pharmacy ; (12): 13-18, 2022.
Article in Chinese | WPRIM | ID: wpr-907006

ABSTRACT

OBJECTIVE To investigate the e ffects of methyl ferulate (MF) on the mitochondrial function of H 9c2 cardiomyocytes after hypoxia-induced injury. METHODS H9c2 cardiomyocytes were divided into normal group (no administration,no modeling ),hypoxia model group (modeling alone ),MF high-dose ,medium-dose and low-dose groups (40, 20,10 μmol/L)and positive control drug group (cyclosporin A ,1 μmol/L). After drug pretreatment and inducing hypoxia-induced injury,the levels of lactate dehydrogenase (LDH),malondialdehyde(MDA),creatine kinase (CK)and adenosine triphosphate (ATP)were tested. The intracellular reactive oxygen species (ROS),mitochondrial membrane potential (MMP),the opening of mitochondrial membrane permeability transition pore (mPTP) were detected with flow cytometry. RESULTS Compared with hypoxia model group ,the levels of LDH ,MDA,CK and ROS fluorescence intensity were decreased significantly in MF high-dose,medium-dose and low-dose groups ,while the level of ATP was increased significantly (P<0.01 or P<0.05). The red/ green fluorescence intensity ratio of MMP and the green fluorescence intensity of mPTP were increased significantly (P<0.01 or P<0.05). CONCLUSIONS MF can reverse the levels of biochemical indexes in H 9c2 cardiomyocyte after hypoxia-induced injury,keep MMP stable ,reduce the opening of mPTP ,and has an obvious protective effect on the mitochondrial function of H9c2 cardiomyocytes injured by hypoxia ,and this protective effect is dose-dependent.

2.
Article in English | WPRIM | ID: wpr-929244

ABSTRACT

Fourteen new geranyl phenyl ethers (1-14) along with three known compounds (15-17) were isolated from Illicium micranthum, and their structures were elucidated by comprehensive spectroscopic methods. Illimicranins A-H (1-8) were characterized as geranyl vanillin ethers, while 9 and 10 were dimethyl acetal derivatives. Illimicranins I and J (11 and 12) were rare geranyl isoeugenol ethers. Illimicranins K and L (13 and 14) represented the first example of geranyl guaiacylacetone ether and geranyl zingerone ether, respectively. Compounds 1, 2 and 15 exhibited anti-HBV (hepatitis B virus) activity against HBsAg (hepatitis B surface antigen) and HBeAg (hepatitis B e antigen) secretion, and HBV DNA replication.


Subject(s)
Antiviral Agents/pharmacology , Hepatitis B Surface Antigens , Hepatitis B e Antigens , Illicium/chemistry , Phenyl Ethers
3.
Acta Physiologica Sinica ; (6): 265-275, 2022.
Article in Chinese | WPRIM | ID: wpr-927602

ABSTRACT

Group 3 innate lymphoid cells (ILC3) as a family member of innate lymphoid cells (ILCs), have been defined as novel innate immune cells in the past decade. ILC3 include a variety of heterogenous subsets with different phenotypes and functions, which are mainly distributed in barrier organs such as the intestine, lung and skin. They play an important role in immune regulation, tissue repair and lymphoid tissue formation. However, in various inflammatory diseases, ILC3 become dysregulated and participate in the pathogenesis through secreting a series of cytokines such as interleukin (IL)-17, IL-22, interferon-γ (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to modulate other immune cells and induce the formation of ectopic lymphoid structures. Therefore, it is of great significance to explore the phenotype and function of ILC3 in order to advance the understanding of inflammatory diseases and find new therapeutic targets. In this article, the phenotypic characteristics, biological functions and research progress of ILC3 in inflammatory diseases were reviewed.


Subject(s)
Cytokines , Immunity, Innate , Interferon-gamma , Intestines , Lymphocytes
4.
International Eye Science ; (12): 997-1001, 2022.
Article in Chinese | WPRIM | ID: wpr-924220

ABSTRACT

@#AIM: To observe the clinical therapeutic efficacy of Ahmed glaucoma valve(AGV)implantation with intravitreal injection of aflibercept or ranibizumab in patients with neovascular glaucoma(NVG). <p>METHODS: A retrospective study. The clinical data of 33 cases(33 eyes)with NVG angle-closure glaucoma who were received intravitreal injection of aflibercept or ranibizumab with AGV implantation from January 2018 to August 2020 in our hospital were involved in this study. There were 18 eyes in the aflibercept group and 15 eyes in the ranibizumab group. All patients were followed-up for 6mo. The best-corrected visual acuity(BCVA), intraocular pressure, neovascularization, and postoperative complications were recorded and evaluated.<p>RESULTS: Before the first intravitreal injection and 1wk after intravitreal injection, the differences of intraocular pressure and BCVA in two groups were not statistically significant(<i>P</i>>0.05); The changes of intraocular pressure and BCVA before the first intravitreal injection and after AGV implant 1wk, 1, 3, 6mo were statistically significant in two groups(<i>P</i><0.01), but there were no statistically significant differences intraocular pressure and BCVA between the two groups at each follow-up time point(<i>P</i>>0.05). There were no significant differences in average number of injections between aflibercept group(1.61±0.98)and ranibizumab group(1.80±0.86)(<i>P</i>>0.05). The differences of neovascular recurrence rate, incidence of early and late complications and surgical success rates were not statistically significant(<i>P</i>>0.05).<p>CONCLUSION: Intravitreal aflibercept or ranibizumab injection combined with AGV implantation is an effective treatment for NVG. The clinical efficacy of aflibercept and ranibizumab were similar.

5.
International Eye Science ; (12): 870-874, 2022.
Article in Chinese | WPRIM | ID: wpr-923430

ABSTRACT

@#AIM: To analyze the epidemiological characteristics of patients hospitalized for ocular trauma during the novel coronavirus(COVID-19)epidemic. <p>METHODS: A retrospective case analysis. Totally 876 cases(905 eyes)of ocular trauma were analyzed in this study, including 545 cases(565 eyes)from January to June 2019 were enrolled ascontrol group, and 331 cases(340 eyes)from January to June 2020 were enrolled in the research group. Information regarding age, sex, location of injury, cause of injury, place of residence, and type of injury were collected and analyzed.<p>RESULTS: The number of hospitalisation for ocular trauma in the research group decreased by 39.3% compared with the control group. The age distribution of patients showed a double peak, at 0-10 years(20.55%, 21.45%)and 41-50 years(17.98%, 19.03%)in the control group and research group, respectively. There was no statistically significant difference in the age distribution between the two groups(<i>P</i>=0.907). Men were predominantly injured in both the control and the research groups, accounting for 80.37% and 83.69%, respectively(<i>P</i>=0.219). The proportion of home injuries in the research group(28.40%)was higher than the control group(11.38%)(<i>P</i><0.001). The proportion of mechanical eye injuries in the research group(98.19%)was higher than the control group(95.60%)(<i>P</i>=0.041), and the proportion of open globe injuries(85.54%)was also higher than the control group(76.58%)(<i>P</i>=0.001). The proportion of patients with no light perception in the research group(18.86%)was higher than that of the control group(9.53%)(<i>P</i><0.001).<p>CONCLUSION: During the COVID-19 epidemic period, the total number of ocular trauma cases was significantly reduced. Mechanical eye injuries are still the main type for hospitalisation of patients with eye injuries. The awareness of eye injury prevention should be strengthened during the period of epidemic.

6.
Article in Chinese | WPRIM | ID: wpr-908454

ABSTRACT

Gallbladder-duodenal fistula is a rare disease in clinical practice, and difficult to diagnosis. One patient with high suspicion of gallbladder-duodenal fistula in preoperative examination was performed with percutaneous transhepatic gallbladder drainage due to could not tolerate surgical operation, and gallbladder-duodenal fistula was diagnosed with the gastric and intestinal fluids extracted from the drainage tube. In the later of fistula repair and the patient′s nutritional support management, the jejunal nutrition tube is inserted through the bile duct, and then the nutrition support was performed through this jejunal nutrition tube. This patients was recovered well.

7.
Article in Chinese | WPRIM | ID: wpr-907630

ABSTRACT

Objective:To study the regulative effect of Rhubarb dispensing granule on autophagy and gastrointestinal motility of Cajal interstitial cells in rats with chronic transit constipation (STC).Methods:A total of 75 rats were randomly divided into control group, model group, low, medium and high dose groups with 15 rats in each group. Except for the control group, the STC rat models were established by intragastric administration of compound diphenoxylate suspension. Rats in low, medium and high dose groups were given Rhubarb dispensing granule of 1, 2 and 4 g/kg by gavage respectively, while rats in control group and model group were given normal saline with the same volume by gavage, once a day for 14 consecutive days. Fecal moisture content and intestinal propulsion rate were measured. The serum levels of substance P (SP), vasoactive intestinal peptide (VIP), NO and NOS were detected by ELISA. The pathological changes of colon tissue were observed by HE staining. The c-kit level in colon tissue was detected by immunohistochemistry. The mRNA and protein levels of c-kit and Beclin1 in colon tissue were detected by PCR and Western blot.Results:Compared with the model group, the fecal moisture content, the carbon pushing distance and the intestinal pushing rate of the low, medium and high dose groups were significantly increased ( P<0.05), the serum SP level was increased ( P<0.05), the serum VIP, NO and NOS levels of the low, medium and high dose groups were decreased ( P<0.05), and the average expression score of c-kit in colon tissue of the low, medium and high dose groups was significantly increased ( P<0.05). The levels of c-kit mRNA (2.33 ± 0.35, 3.04 ± 0.17, 3.83 ± 0.23 vs. 0.61 ± 0.07) and protein (0.42 ± 0.06, 0.60 ± 0.07, 0.79 ± 0.08 vs. 0.22 ± 0.04)in colon tissue of rats in low, medium and high dose groups were increased ( P<0.05), and Beclin1 mRNA (4.17 ± 0.37, 3.35 ± 0.44, 1.05 ± 0.28 vs. 6.04 ± 0.31) and protein (0.76 ± 0.11, 0.57 ± 0.08, 0.43 ± 0.05 vs. 0.91 ± 0.06) were decreased ( P<0.05). Conclusion:Rhubarb dispensing granule could significantly increase the fecal moisture, intestinal motility rate, serum SP level and colonic tissue c-kit level, decrease serum VIP, NO, NOS level and colonic tissue Beclin1 level in rats with chronic transit constipation, and then inhibit autophagy of Cajal interstitial cells and regulate gastrointestinal motility in rats with chronic transit constipation.

8.
Article in Chinese | WPRIM | ID: wpr-906616

ABSTRACT

Objective To explore the epidemiological characteristics and the potential years of life lost of gynecological malignant tumors among female residents in Pudong New Area of Shanghai between 2002 and 2019. Methods Crude mortality rate (CMR), age-standardized mortality rate (SMR), potential years of life lost (PYLL), average years of life lost (AYLL) and annual percent change (APC) of the cervical cancer,uterine cancer and ovarian cancer deaths were calculated to analyze the mortality situation of gynecological malignant tumors among female residents in Pudong New Area. Results The crude mortality rate were 2.65/100 000, 2.44/100 000 , 4.55/100 000, and age-standardized mortality rate were 1.33/100 000, 1.06/100 000, 2.26/100 000, respectively, among female residents in Pudong New Area during 2002 to 2019. In the study period, both the crude mortality rate and the age-standardized mortality rate of cervical cancer rose over the years; Both the crude mortality rate and the age-standardized mortality rate of uterine cancer kept stable; The crude mortality rate of ovarian cancer showed an upward trend, and the age-standardized mortality rate kept stable. The PYLL of cervical cancer was 7335 years, the PYLL rate was 0.30‰, and the AYLL was 11.20 years per person; The PYLL of uterine cancer was 3556 years, the PYLL rate was 0.14‰, and the AYLL was 5.90 years per person; The PYLL of uterine cancer was 10017 years, the PYLL rate was 0.41‰, and the AYLL was 8.91 years per person. Conclusion The mortality rate of cervical cancer and ovarian cancer rose over years, and the mortality of uterine cancer kept stable among female residents in Pudong New Area during 2002 to 2019. The gynecological malignant tumors caused severe disease burden.

9.
National Journal of Andrology ; (12): 927-933, 2021.
Article in Chinese | WPRIM | ID: wpr-922177

ABSTRACT

Prostate cancer (PCa) is a maligmancy with high morbidity and mortality. Bone metastasis is the main cause of short survival time and difficulties in the treatment and prevention of PCa. Previous findings of our team showed 155 bone-specific genes highly expressed in bone metastatic PC3 cells, which is considered to be the key to their adaptation to the bone micro-environment, proliferation and formation of metastatic tumor, and extensively exists in cancer metastasis in multiple systems. This review summarizes the published literature on the highly expressed bone-specific genes, focusing on the roles and values of these genes in the metastasis, progression, clinical diagnosis, treatment and prognosis of PCa, offering a prospect of the direction and targets in the studies of PCa bone metastasis so as to enrich the bone metastatic theories and clinical treatment principles of this disease in the future.


Subject(s)
Humans , Male , PC-3 Cells , Prostatic Neoplasms/genetics , Tumor Microenvironment
10.
Article in English | WPRIM | ID: wpr-888669

ABSTRACT

OBJECTIVE@#To investigate the effects of multidisciplinary and comprehensive Chinese medicine (CM) treatments on progression-free survival (PFS) and median survival time (MST) in patients with advanced non-small cell lung cancer (NSCLC) and identify factors that influence progression and prognosis.@*METHODS@#Clinical data of 855 patients with advanced NSCLC who received multidisciplinary and comprehensive CM treatments at Longhua Hospital from January 2009 to December 2018 were retrospectively analyzed. Univariate analysis was performed by the Kaplan-Meier method and log-rank sequential inspection. Multivariate analysis of significant variables from the univariate analysis was performed with Cox regression modeling. Key factors correlated to progression and prognosis were screened out, and a Cox proportional hazard model was established to calculate the prognostic index.@*RESULTS@#The PFS and MST of 855 advanced NSCLC patients were 9.0 and 26.0 months, respectively. The 1-, 2-, 3-, and 5-year survival rates were 79.2%, 54%, 36.2%, and 17.1%, respectively. Gender, pathologic type, and clinical stage were independent prognostic risk factors; surgical history, radiotherapy, treatment course of Chinese patent medicine, intravenous drip of Chinese herbal preparation, duration of oral administration of Chinese herbal decoction (CHD), and intervention measures were independent prognostic protective factors. Gender was an independent risk factor for progression, while operation history and oral CHD administration duration were independent protective factors (all P<0.05). Women with stage IIIb-IIIc lung adenocarcinoma had the best outcomes.@*CONCLUSIONS@#Female patients have lower progression risk and better prognoses than male patients, younger patients have higher progression risk but better long-term prognoses than the elderlys, and patients with lower performance status scores are at lower risk for progression and have better prognoses. Comprehensive CM treatments could significantly reduce progression risk, improve prognosis, and prolong survival time for patients with advanced NSCLC. This treatment mode offers additional advantages over supportive care alone.

11.
Article in English | WPRIM | ID: wpr-921335

ABSTRACT

Thalassemia is a group of genetically heterogeneous diseases characterized by hemolytic anemia. To investigate molecular characteristics of α- and β-thalassemia among young individuals of marriageable age in Guangdong Province, 24,788 subjects with suspected thalassemia were genetically tested for α- and β-thalassemia by Gap-PCR and reverse dot blot during 2018-2019. For suspected rare thalassemia cases, DNA sequencing was performed to identify rare and unknown thalassemia gene mutations. A total of 14,346 thalassemia carriers were detected, including 7,556 cases of α-thalassemia with 25 genotypes and 8 α-gene mutations identified, 5,860 cases of β-thalassemia with 18 genotypes and 18 β-gene mutations identified, and 930 cases of compound α/β-thalassemia. Among them, the frequency of --


Subject(s)
Adult , China , Female , Genotype , Humans , Male , Middle Aged , Mutation , Sequence Analysis, DNA , Young Adult , alpha-Thalassemia/genetics , beta-Thalassemia/genetics
12.
Article in Chinese | WPRIM | ID: wpr-911272

ABSTRACT

Objective:To evaluate the efficacy of preoperative interview with humanistic care in patients undergoing resection of tumor.Methods:Two hundred patients, aged 35-64 yr, with body mass index of 18-28 kg/m 2, of American Society Anesthesiologists physical status Ⅰ or Ⅱ, undergoing elective laparoscopic gastrointestinal tumor resection, were divided into 2 groups ( n=100 each) by a random number table method: conventional preoperative interview group (group C) and preoperative interview with humanistic care group (group H). Pre-interview anxiety was assessed using the Self-Rating Anxiety Scale (SAS) at 1 day before the interview (T 0), and pre-interview heart rate (HR) and mean arterial pressure (MAP) were recorded, followed by a preoperative interview.Conventional preoperative interview was given in group C, and preoperative interview with humanistic care was given in group H. Anxiety was assessed again after interview (T 1) and HR and MAP were recorded at 24 h. Venous blood samples were collected for determination of plasma norepinephrine (NE) and cortisol (Cor) concentrations by enzyme-linked immunosorbent assay at T 0 and T 1. Results:Compared with group C, the MAP, HR and SAS scores were significantly decreased at T 1, the incidence and degree of anxiety and plasma NE and Cor concentrations were decreased at T 1, and the incidence of abnormalities of MAP, HR and plasma Cor concentrations were decreased in group H ( P<0.05). Conclusion:Preoperative interview with humanistic care can alleviate the post-interview anxiety and reduce the over-stress response in patients undergoing resection of tumor.

13.
Chinese Journal of Orthopaedics ; (12): 929-937, 2021.
Article in Chinese | WPRIM | ID: wpr-910675

ABSTRACT

Objective:To perform a prospective cohort study to identify individual susceptibility of glucocorticoid (GC) -associated osteonecrosis of the femoral head (GA-ONFH) and their clinical and genetic risk factors. Methods:The present prospective cohort study enrolled patients who received their first GC therapy between July 2015 and January 2018 at Zhongshan Hospital. All patients did not receive any GC treatment before enrollment. Further, they planned to start GC treatment with the dose (equivalent prednisone) of ≥30 mg/d, lasted ≥3 weeks, or pulse dose ≥200 mg/d, lasted ≥3 d. Blood samples were collected before GC treatment to evaluate bone metabolism and its released factors. Hip MRI was performed at the 1st, 3rd, 6th, 12th and 24th month to diagnose GA-ONFH. All patients were followed-up for ≥2 years. The endpoint was regarded as diagnosis of GA-ONFH or completion of 2 years follow-up. Lasso regression was performed to determine which clinical features were associated with GA-ONFH. A nested case-control sub-cohort (A, n=12) was established prospectively based on the main cohort by 1∶1 matching. Whole exome sequencing was performed to screen differential and functional candidate single nucleotide polymorphisms and insertion-deletions (SNP/InDels). Another sub-cohort (B, n=50) was constructed retrospectively in patients with GA-ONFH and non-ONFH patients received standard high dose GC treatment for more than two years. The candidate SNP/InDels were verified by Sanger sequencing based on the patients from sub-cohort B. Results:A total of 96 patients were enrolled of which 88 of them (32 males and 56 females, mean age 42.30 years) completed follow-up. Eight cases (9.1%) were diagnosed with GA-ONFH. The median time from the start of GC therapy to the diagnosis of ONFH was 53.00(34.00,13.50) days. The baseline characteristics, such as age, sex and body mass index, indicated no significant difference between the ONFH group and the non-ONFH group. The cumulative GC dose of the ONFH patients in the first month was higher than that of non-ONFH [32.74(29.55, 47.05) mg/kg vs. 24.00(21.10, 29.45) mg/kg, Z=-2.410, P=0.016]. However, there was no significant difference of patients who underwent pulse therapy (37.5% vs. 10.0%, adjusted χ 2=2.829, P=0.093). The ratio of serum apolipoprotein B/apolipoprotein A1 (ApoB/ApoA1) in patients with ONFH was higher than that in non-ONFH group before GC use [0.95(0.80, 1.50) vs. 0.70(0.60, 0.80), Z=-2.875, P=0.000]. Due to the multicollinearity, Lasso regression model was performed to reduce overfitting. All variables were included in the model. The results suggested that higher ApoB/ApoA1 ratio, lower serum β-c-terminal telopeptide (β-CTX) and higher cumulative GC dose in the first month were the top three risk factors of GA-ONFH. This model had an accuracy of 0.982 in internal validation. Seven differential candidate SNP/InDels were found by whole exome sequencing of sub-cohort A. We further verified these SNP/InDels in sub-cohort B. The patients with COLEC12 mutation (rs2305027, G1816A) were at risk of GA-ONFH ( OR=6.00, 95% CI: 1.17, 30.73). Conclusion:Higher first-month GC dose, lower serum β-CTX level before treatment, higher ApoB/ApoA1 ratio and COLEC12 mutation (rs2305027, G1816A) could increase the risk of GA-ONFH.

14.
Article in Chinese | WPRIM | ID: wpr-906331

ABSTRACT

Objective:To explore the active components, targets, and signaling pathways responsible for Bushen Zhuyun prescription in treating the recurrent spontaneous abortion (RSA) based on network pharmacology and uncover its potential mechanism by molecular docking and in vitro cell experiments. Method:The active components of Bushen Zhuyun prescription were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Traditional Chinese Medicine Integrated Database (TCMID) and the published articles, followed by the prediction of drug action targets based on such platforms as DrugBank and SwissTargetPrediction. GeneCards and Online Mendelian Inheritance in Man (OMIM) were searched to obtain the RSA targets, which were then intersected with the targets of Bushen Zhuyun Decoction. Following the plotting of Bushen Zhuyun prescription-compound-target-RSA network by Cytoscape 3.7.1, the protein-protein interaction (PPI) network was then constructed with STRING for screening the core network. The resulting common targets were then subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis using R software. Autodock Vina 1.1.2 was used for molecular docking. The activation of phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway by Bushen Zhuyun prescription was verified in human umbilical vein endothelial cells (HUVEC) <italic>in vitro</italic>. Result:It was found that 49 potential active components of Bushen Zhuyun prescription might act on 133 RSA targets. GO enrichment analysis yielded 470 biological processes, with angiogenesis, vascular development, cellular proliferation, and oxidative activity mainly involved. KEGG enrichment analysis revealed 103 signaling pathways (<italic>P</italic><0.05), and the PI3K/AKT signaling pathway, advanced glycation end product (AGE)/receptor for advanced glycation end product (RAGE) signaling pathway, and tumor necrosis factor (TNF) signaling pathway were the main ones. As indicated by molecular docking, the Vina scores of the main active component kaempferol with AKT1 and vascular endothelial growth factor A (VEGFA) were the lowest and similar. It was confirmed <italic>in vitro</italic> cell experiments that Bushen Zhuyun prescription activated the PI3K/AKT signaling pathway and up-regulated the expression of VEGFA and downstream AKT protein to promote angiogenesis. Conclusion:Bushen Zhuyun prescription promotes angiogenesis at the maternal-fetal interface by regulating angiogenesis and cellular proliferation, activating the PI3K/AKT pathway, and up-regulated the VEGFA expression, which is beneficial to the formation of placenta in early pregnancy and the maintenance of early pregnancy. This study has provided ideas for new drug development.

15.
Article in Chinese | WPRIM | ID: wpr-906237

ABSTRACT

Objective:To explore the intervention effect of Wenxin granule on endoplasmic reticulum stress apoptosis pathway and its related mechanism in rats with myocardial infarction. Method:Ligating the left anterior descending coronary artery to establish the rat model, the rats were randomly divided into 5 groups, namely sham group, model group, betaloc group and low, high-dose Wenxin granule groups,10 in each group. The sham group and the model group were given 10 mL∙kg<sup>-1</sup>∙d<sup>-1</sup> deionized water, the low, high-dose modified Wenxin groups were given 1.35, 2.7 g∙kg<sup>-1</sup>∙d<sup>-1</sup> aqueous solution respectively, and the betaloc group was given 2.25 mg∙kg<sup>-1</sup>∙d<sup>-1</sup> aqueous solution. After 14 days,the catheter method was used to detect the cardiac hemodynamics and hematoxylin-eosin staining (HE) was used to observe the pathological morphological changes. The levels of endoplasmic reticulum stress protein glucose regulatory protein 78(GRP78), protein kinase R like endoplasmic reticulum kinase(PERK), phosphorylated activated PERK(p-PERK), activated transcription factor 6 (ATF6), nuclear transcription factor X cassette binding protein(XBP1) and apoptosis protein C/EBP homologous protein(CHOP), B cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated X protein(Bax) were detected by Western blot. Deoxyribonucleotide end-transferase-mediated notch end labeling (TUNEL) was also used to detect the myocardial cell apoptosis. Result:Compared with control group, the levels of the maximum ascending rate of left ventricular pressure(+dp/dt<sub>max</sub>), the maximum descending rate of left ventricular pressure(-dp/dt<sub>max</sub>) and the left ventricular systolic blood pressure(LVSP) were decreased significantly(<italic>P</italic><0.05),but the level of the left ventricular end diastolic blood pressure (LVEDP) was significantly increased(<italic>P</italic><0.05). The expressions of GRP78, p-PERK, PERK, ATF6, XBP1, CHOP, Bax and apoptosis index were increased significantly (<italic>P</italic><0.05,<italic>P</italic><0.01), while Bcl-2/Bax and Bcl-2 were decreased significantly(<italic>P</italic><0.01) in model group. Compared with the model group, the levels of -dp/dt<sub>max</sub> and Bcl-2 were increased significantly (<italic>P</italic><0.05),while the level of the apoptosis index was decreased significantly(<italic>P</italic><0.01) in low-dose Wenxin granule group. In high-dose Wenxin granule group, the levels of +dp/dt<sub>max</sub> and -dp/dt<sub>max</sub> were increased significantly(<italic>P</italic><0.05,<italic>P</italic><0.01), the levels of endoplasmic reticulum stress pathway protein GRP78, p-PERK, PERK, ATF6, XBP1,apoptosis-related protein CHOP, Bax and apoptosis index were decreased significantly(<italic>P</italic><0.01), but Bcl-2 and Bcl-2/Bax were increased significantly (<italic>P</italic><0.01). Conclusion:Wenxin granule has effect in inhibiting excessive endoplasmic reticulum stress, reducing myocardial cell apoptosis and improving cardiac hemodynamics. Its molecular mechanism may be related to decrease the levels of GRP78, PERK, p-PERK, ATF6, XBP1, CHOP, Bax and increasing the expression of Bcl-2.

16.
Article in Chinese | WPRIM | ID: wpr-905946

ABSTRACT

Autism spectrum disorder (ASD) is a common neurodevelopmental disorder in childhood, whereas there is no specific medicine at present. There are more and more researches on the treatment of ASD with traditional Chinese medicine(TCM) which the curative effect is reliable. The heart and spleen are the main viscera for the treatment of ASD, but there is still a lack of in-depth analysis of the mechanism of TCM. In order to explore the relationship between the core symptoms of ASD and the heart and spleen, this article specifically explores the theoretical origins of the heart and the spleen in the formation of the core symptoms of ASD, and to clarify the role of the heart and spleen in the occurrence and development of the two core symptoms of ASD from the perspective of TCM. In view of social communication and communication obstacles, the author puts forward and explains the language problems of children with ASD based on the functions of the heart and spleen, the theory of the viscera, the ascription of the meridians, and the classics. The mechanism of the heart and spleen in TCM about the failure of the spleen, the loss of the heart, and the endogenous phlegm. Aiming at the mechanism of the stereotyped symptoms of abnormal behaviors in children with ASD, this paper proposes and explains the TCM mechanism of constant deficiency of the spleen and dereliction of duty, leading to loss of mind, heart and spleen injury, and finally a series of stereotypes and strange syndromes due to lack of spirit. Through the analysis and excavation of TCM theory, it explores theoretical basis for ASD from the theory of heart and spleen, with a view to preliminarily constructing the theoretical framework of TCM syndrome differentiation and treatment of the deficiency of both the heart and spleen, and provide theoretical reference for TCM syndrome differentiation and treatment of ASD. The treatment of ASD from the differentiation of symptoms and signs of the heart and spleen is supported by a strong theoretical basis of TCM, and the rationale, law and prescriptions are complete, which may be the direction of screening effective TCM prescriptions for the treatment of ASD in the future.

17.
Article in Chinese | WPRIM | ID: wpr-887899

ABSTRACT

Objective To investigate the oral health status and awareness of urban children in Lhasa,aiming to provide a data basis for the prevention and treatment of children's caries and the promotion of oral health education. Methods A total of 504 Tibetan students were selected by cluster sampling from 2 primary schools in Chengguan District of Lhasa.All the participants were required to take oral health examination and complete a questionnaire about oral health awareness and behavior. Results The caries prevalence rate and mean decayed-missing-filled tooth(DMFT)of permanent teeth were 75.00% and 2.18±1.91,respectively.The rates of pit and fissure sealant and filling of permanent teeth were 3.77% and 6.81%,respectively.The caries prevalence rate of first permanent molars was 47.62%.The mean DMFT of permanent teeth and caries prevalence rate of first permanent molar were significantly higher in female group(


Subject(s)
Child , DMF Index , Dental Caries/epidemiology , Female , Humans , Oral Health , Oral Hygiene , Prevalence , Schools , Students , Surveys and Questionnaires
18.
Article in English | WPRIM | ID: wpr-887746

ABSTRACT

Beta (β)-thalassemia is one of the most common hemoglobinopathies worldwide, creating major public health problems and social burdens in many regions. Screening for β-thalassemia carriers is crucial for controlling this condition. To investigate the effectiveness of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) for screening β-thalassemia, retrospective data were analyzed for 6,779 β-thalassemia carriers subjected to genetic testing following thalassemia screening in Guangdong province between January 2018 and December 2019. Prevalent mutations observed included CD41/42 (-TTCT) (38.43%), IVS-II-654 (C > T) (25.71%), -28 (A > G) (15.78%), CD17 (AAG > TAG) (10.03%), and β


Subject(s)
Adult , China , Erythrocyte Indices , Female , Humans , Male , Mass Screening , Middle Aged , Mutation , Young Adult , beta-Thalassemia/genetics
19.
Chinese Medical Journal ; (24): 1552-1560, 2021.
Article in English | WPRIM | ID: wpr-887612

ABSTRACT

BACKGROUND@#Chronic obstructive pulmonary disease (COPD) is a common public health problem worldwide. Recent studies have reported that socioeconomic status (SES) is related to the incidence of COPD. This study aimed to investigate the association between SES and COPD among adults in Jiangsu province, China, and to determine the possible direct and indirect effects of SES on the morbidity of COPD.@*METHODS@#A cross-sectional study was conducted among adults aged 40 years and above between May and December of 2015 in Jiangsu province, China. Participants were selected using a multistage sampling approach. COPD, the outcome variable, was diagnosed by physicians based on spirometry, respiratory symptoms, and risk factors. Education, occupation, and monthly family average income (FAI) were used to separately indicate SES as the explanatory variable. Mixed-effects logistic regression models were introduced to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for examining the SES-COPD relationship. A pathway analysis was conducted to further explore the pulmonary function impairment of patients with different SES.@*RESULTS@#The mean age of the 2421 participants was 56.63 ± 9.62 years. The prevalence of COPD was 11.8% (95% CI: 10.5%-13.1%) among the overall sample population. After adjustment for age, gender, residence, outdoor and indoor air pollution, body weight status, cigarette smoking, and potential study area-level clustering effects, educational attainment was negatively associated with COPD prevalence in men; white collars were at lower risk (OR: 0.60, 95% CI: 0.43-0.83) of experiencing COPD than blue collars; compared with those within the lower FAI subgroup, participants in the upper (OR: 0.68, 95% CI: 0.49-0.97) tertiles were less likely to experience COPD. Such negative associations between all these three SES indicators and COPD were significant among men only. Education, FAI, and occupation had direct or indirect effects on pulmonary function including post-bronchodilator forced expiratory volume in 1 s/forced vital capacity (FEV1/FVC), FEV1, FVC, and FEV1 percentage of predicted. Education, FAI, and occupation had indirect effects on pulmonary function indices of all participants mainly through smoking status, indoor air pollution, and outdoor air pollution. We also found that occupation could affect post-bronchodilator FEV1/FVC through body mass index.@*CONCLUSIONS@#Education, occupation, and FAI had an adverse relationship with COPD prevalence in Jiangsu province, China. SES has both direct and indirect associations with pulmonary function impairment. SES is of great significance for COPD morbidity. It is important that population-based COPD prevention strategies should be tailored for people with different SES.


Subject(s)
Adult , Aged , China/epidemiology , Cross-Sectional Studies , Forced Expiratory Volume , Humans , Male , Middle Aged , Prevalence , Pulmonary Disease, Chronic Obstructive/epidemiology , Risk Factors , Social Class , Spirometry , Vital Capacity
20.
Article in Chinese | WPRIM | ID: wpr-887143

ABSTRACT

Objective:To analyze the injury characteristics and the burden of disease for developing an injury prevention strategy. Methods:Data derived from the ‘National Injury Monitoring Report Card’ and the Global Burden of Diseases Study 2017(GBD 2017) was analyzed for the relevant indicators including proportion, disability adjusted of life years(DALY), DALY rate,et al. Results:The information of 91 507 cases was analyzed. Male cases were more than the females with sex ratio of 1.5∶1. The highest injury rate (38.1%) was in the group of 25-44 years old. The major career structure was production and transportation (25.5%). The education level of most injured people was middle school. The top two causes of injuries were falls and traffic-related. In 2017, a total of 44 725 people died of injury and the crude death rate of injury was 54.77/105, 64.51/105 in male and 44.85/105 in female. In 2017, the DALY and DALY rate caused by injuries was 217.8 thousand person-years and 2 667.27/105. The top two injuries of DALYs were road traffic accidents and falls, followed by suicide, drowning and mechanical injury. Conclusion:Targeted prevention and control measures for road traffic injury and falls injury should be in place to reduce the burden of injury.

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