Your browser doesn't support javascript.
Show: 20 | 50 | 100
Results 1 - 5 de 5
Add filters

Year range
Chinese Journal of Cellular and Molecular Immunology ; (12): 952-958, 2023.
Article in Chinese | WPRIM | ID: wpr-1009453


Natural killer (NK) cells are an important part of the body's innate immune system. As the first line of defense against pathogens, they need to be transformed into a mature state under the control of various cell signaling molecules and transcription factors to play cytotoxic and immune regulatory roles. Under the interaction of activated receptors and inhibitory receptors, NK cells are activated to perform a direct cell killing effect by secreting perforin and granzyme, or indirectly eliminate pathogenic microorganisms in the body by secreting various cytokines, such as type I and type II interferons. These functions of NK cells play a very important role in antiviral and anti-autoimmune diseases, especially in anti-tumor.

Humans , Killer Cells, Natural , Interferon-gamma , Apoptosis , Autoimmune Diseases , Cytokines
Acta Pharmaceutica Sinica B ; (6): 2926-2954, 2023.
Article in English | WPRIM | ID: wpr-982904


Sonodynamic therapy (SDT) is an emerging noninvasive treatment modality that utilizes low-frequency and low-intensity ultrasound (US) to trigger sensitizers to kill tumor cells with reactive oxygen species (ROS). Although SDT has attracted much attention for its properties including high tumor specificity and deep tissue penetration, its anticancer efficacy is still far from satisfactory. As a result, new strategies such as gas-assisted therapy have been proposed to further promote the effectiveness of SDT. In this review, the mechanisms of SDT and gas-assisted SDT are first summarized. Then, the applications of gas-assisted SDT for cancer therapy are introduced and categorized by gas types. Next, therapeutic systems for SDT that can realize real-time imaging are further presented. Finally, the challenges and perspectives of gas-assisted SDT for future clinical applications are discussed.

Chinese Journal of Biotechnology ; (12): 882-892, 2022.
Article in Chinese | WPRIM | ID: wpr-927751


With the widespread application of genomics and transcriptomics in the genetics and cell biology of different species, synonymous codon usage bias has been gradually accepted and used to study the deep connection between biological evolution and biological phenotypes. It is an important part of the life activities that mRNA is expressed into proteins with normal biological activities. The synonymous codon usage patterns, which were named as 'the second genetic codon', can express genetic information carried by themselves at the levels of transcriptional regulations, translational regulations and metabolic activities through molecular mechanisms such as fine-tune translation selection. Some studies have shown that the length of mRNA half-life has significant impacts on mRNA activity and the process of transcription and translation. This review summarized the roles of synonymous codon usage patterns in transcription, translational regulation and post-translational modification, with the aim to better understand how organisms skillfully utilize the genetic effects caused by codon usage patterns to accurately synthesize different types of proteins, so as to ensure the growth or differentiation of the specific gene expression procedures to carry out smoothly and maintain the normal life cycle.

Codon/genetics , Codon Usage , Half-Life , Protein Processing, Post-Translational , RNA, Messenger/genetics
Chinese Journal of Anesthesiology ; (12): 1091-1093, 2012.
Article in Chinese | WPRIM | ID: wpr-430835


Objective To investigate the efficacy of preemptive analgesia with parecoxib sodium combined with local infiltration anesthesia with ropivacaine for postoperative pain after laparoscopic cholecystectomy.Methods One hundred and fifty ASA Ⅰ or Ⅱ patients,aged 41-63 yr,weighing 55-87 kg,scheduled for laparoscopic cholecystectomy under the general anesthesia,were randomly divided into 3 groups (n=50 each): parecoxib sodium+0.9% normal saline (group A),parecoxib sodium + 0.5% ropivacaine (group B) and parecoxib sodium + 0.75 % ropivacaine (group C).Parecoxib sodium 40 mg was injected intravenously 30 min before induction of anesthesia.Anesthesia was induced and maintained with remifentanil and propofol given by TCI.Immediately before skin closure,0.9% normal saline 12 ml was given in group A,and 0.5% and 0.75% ropivacaine 12 ml were injected for local infiltration anesthesia in groups B and C,respectively.VAS scores were maintained ≤ 3after operation.When VAS scores > 3,pethidine 75 mg was injected intramuscularly.Ramsay sedation scores were recorded at the end of operation and 2,4,8,12 and 24 h after operation.The side effects (nausea and vomiting,allergy,respiratory depression,etc.),requirement for pethidine,algesic sites (incisional pain,upper abdominal pain,referred pain in right shoulder),and condition of the wound healing were all recorded within 24 h after opcration.Results Compared with group A,Ramsay sedation scores,the incidence of nausea and vomiting,the requirement for pethidine and total amount of pethidine,and the incidence of incisional pain were significantly decreased in groups B and C (P < 0.05 or 0.01).Compared with group B,the requirement for pethidine and total amount of pethidine,and the incidence of incisional pain were significantly decreased in group C (P < 0.05 or 0.01).There was no significant difference in Ramsay sedation scores between groups B and C (P > 0.05).There was no significant difference in the condition of the wound healing,upper abdominal pain and referred pain in right shoulder between groups A,B and C (P > 0.05).Conclusion For the patients scheduled for laparoscopic cholecystectomy,local infiltration anesthesia with ropivacaine can improve the efficacy of preemptive analgesia with parecoxib sodium,and it provides better postoperative analgesia when 0.75 % ropivacaine is used.

Chinese Journal of Anesthesiology ; (12)1994.
Article in Chinese | WPRIM | ID: wpr-522811


65 mm Hg and CVP between 10-14 cm H2O. In control group no AHHD was performed. Blood samples and urine specimens were obtained before anesthesia (T1), at the end of operation (T2 ) and 72 h after operation (T3 ) for determination of blood and urinary creatinine (BCr, UCr) , blood sodium (BNa) , urinary sodium (UNa), blood urea nitrogen (BUN) , urinary ?1-microglobulin (?1-MG), urinary albumin, urinary immuno-globulin G, creatinine clearance (CCr), and fractional sodium clearance (FENa) . Results BUN, urinary albumin, urinary immuno-globulin G, CCr and FENa were normal during the study in both groups. Urinary concentration of ?1-MG was significantly increased at T2 as compared with the baseline value before anesthesia (T, ) and declined at T3 but was still significantly higher than that at T1 . There was no significant difference between the two groups in terms of the parameters mentioned above. Conclusion AHHD with 6% HES (200/0.5) has no detrimental effects on renal function in patients with normal function.