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1.
Article in Chinese | WPRIM | ID: wpr-921699

ABSTRACT

Whitmania pigra is the most widely distributed species of leeches in the market. In this study, the effect of heavy metal lead pollution on the anticoagulant activity of Wh. pigra was studied and the potential mechanism was explored. Pb(NO_3)_2 was used to contaminate the breeding soil which was then used to rear Wh. pigra for 50 days(lead-contaminated group, LC group), and meanwhile the blank control group(CG group) was set. Proteins were extracted from the obtained leech samples, and the differentially expressed proteins between LC and CG groups were analyzed by label-free proteomics technology. In this study, a total of 152 differentially expressed proteins were screened out, of which 93 proteins were up-regulated and 59 proteins were down-regulated in LC group. Bioinformatics analysis showed that the biological processes enriched with the differentially expressed proteins were mainly vesicle-mediated transport and transport positive regulation; the enriched cell components were mainly endocytosis vesicles and apical plasma membrane; the enriched molecular functions mainly included carbohydrate binding. The differentially expressed proteins were enriched in 76 KEGG pathways, which mainly involved metabolic pathways, biosynthesis of secondary metabolites, and bacterial invasion of epithelial cells. In this study, two differentially expressed proteins with Antistasin domain were presumed, which provides reference for further exploring the regulatory mechanism and signal transduction underlying the effect of lead pollution on the anticoagulant activity of leech.


Subject(s)
Animals , Anticoagulants/pharmacology , Environmental Pollution , Leeches , Metals, Heavy , Proteomics
2.
Chinese Medical Journal ; (24): 2850-2856, 2021.
Article in English | WPRIM | ID: wpr-921172

ABSTRACT

BACKGROUND@#Central nervous system (CNS) symptoms after efavirenz (EFV) treatment in people living with human immunodeficiency virus (HIV) could persist and impact their quality of life. We assessed the impact of EFV-based regimen replacement with elvitegravir/cobicistat/emtricitabine/tenofovir alafenamide (E/C/F/TAF), which is considered an alternative option for subjects who do not tolerate EFV. Most specifically, we assessed the safety and the efficacy of E/C/F/TAF and its effects on the participants' neuropsychiatric toxicity symptoms in a real-life setting.@*METHODS@#A prospective cohort study was conducted among virologic suppressed HIV-positive participants receiving EFV-based regimens with ongoing CNS toxicity ≥ grade 2. The participants were switched to single-pill combination regimens E/C/F/TAF and followed up for 48 weeks. The neuropsychiatric toxicity symptoms were measured using a CNS side effects questionnaire, as well as the Hospital Anxiety and Depression Scale and the Pittsburgh Sleep Quality Index. The primary outcome measure was the proportion of participants experiencing grade 2 or higher CNS toxicity after EFV switch off at weeks 12, 24, and 48. Secondary endpoints included virologic and immunological responses and the effect on fasting lipids at week 48 after switch.@*RESULTS@#One hundred ninety-six participants (96.9% men, median age: 37.5 years, median: 3.7 years on prior EFV-containing regimens) were included in the study. Significant improvements in anxiety and sleep disturbance symptoms were observed at 12, 24, and 48 weeks after switching to E/C/F/TAF (P < 0.05). No significant change in depression symptom scores was observed. At 48 weeks after switch, HIV viral load <50 copies/mL was maintained in all of the participants, median fasting lipid levels were moderately increased (total cholesterol [TC]: 8.2 mg/dL, low-density lipoprotein cholesterol [LDL-C]: 8.5 mg/dL, high-density lipoprotein cholesterol [HDL-C]: 2.9 mg/dL, and triglyceride (TG): 1.6 mg/dL, and the TC:HDL-C ratio remained stable.@*CONCLUSIONS@#The single-pill combination regimens E/C/F/TAF is safe and well tolerated. This study reveals that switching from EFV to E/C/F/TAF significantly reduces neuropsychiatric toxicity symptoms in people living with HIV with grade 2 or higher CNS complaints.


Subject(s)
Adenine/therapeutic use , Adult , Alanine , Alkynes , Anti-HIV Agents/adverse effects , Benzoxazines , Central Nervous System , Cobicistat/therapeutic use , Cyclopropanes , Drug Combinations , Emtricitabine/therapeutic use , Female , HIV Infections/drug therapy , Humans , Male , Prospective Studies , Quality of Life , Quinolones , Tenofovir/analogs & derivatives
3.
Article in Chinese | WPRIM | ID: wpr-906314

ABSTRACT

In recent years, the incidence of neurological diseases has been increasing year by year. To give full play to the advantages of traditional Chinese medicine (TCM) in the treatment of neurological disorders, identify the breakthrough point of integrating TCM with western medicine, and further standardize the clinical diagnosis and treatment of TCM, the China Association of Chinese Medicine organized neurologists in TCM and western medicine to carry out in-depth discussion on the neurological diseases responding specifically to TCM and integrated TCM and western medicine, such as stroke, headache, vertigo, multiple sclerosis, and epilepsy, aiming to formulate a well-recognized and integrated treatment protocol for TCM and western medicine and improve the efficacy of neurological disorders. Furthermore, the treatment suggestions of the corresponding diseases in TCM and western medicine were proposed to provide references for clinical practice and scientific research.

4.
Chinese Journal of School Health ; (12): 1068-1072, 2021.
Article in Chinese | WPRIM | ID: wpr-886327

ABSTRACT

Objective@#To explore the association between growth patterns and blood pressure in children and adolescents with different nutritional status.@*Methods@#A total of 38 839 children and adolescents aged 6 to 8 years old were included in this study by stratified cluster sampling. The American Academy of Pediatrics 2017 Guideline was used to evaluate the blood pressure, the US 2000 CDC standard was used to determine different growth patterns, and the WHO Child and Adolescent Growth and Development Standard issued in 2007 was used to evaluate nutritional status. Variance analyses were used to compare the levels of systolic and diastolic blood pressure and growth patterns by nutritional status, and χ 2 test was used to compare the difference of prevalence. Multivariate Logistic regression model was used to analyze the association between growth patterns and blood pressure.@*Results@#The proportion of normal growth, catch up growth and catch down growth was 33.2%, 41.6% and 25.2%, respectively. Under different growth patterns, systolic blood pressure (105.17±12.33) mm Hg and diastolic blood pressure (66.55±8.75) mm Hg of catch up growth were higher than those of normal growth and catch down growth. In overweight and obesity, the prevalence of hypertension (24.9%), high systolic blood pressure (18.9%) and high diastolic blood pressure (15.0%) in catch up growth were higher than those in normal growth and catch down growth ( P <0.05). The risk of catch up growth to hypertension was higher in overweight and obesity ( OR =1.18, 95% CI =1.06-1.31) than in normal children and adolescents ( OR =1.15, 95% CI =1.05-1.27).@*Conclusion@#In catch up growth children and adolescents, hypertension and high blood pressure are higher than normal growth and catch down growth. Overweight and obesity than normal children and adolescents have a higher risk of hypertension.

5.
Article in Chinese | WPRIM | ID: wpr-885562

ABSTRACT

Thyroid diseases in fetuses and newborns are rare but can be severe in some cases. Early diagnosis and treatment are the keys to improve the prognosis. This review focuses on the diagnosis and treatment strategies of this disease during the fetal and neonatal periods. For fetuses with goiter, the main clinical issue is to differentiate hyperthyroidism or hypothyroidism and offer appropriate management on this basis. Management of maternal, fetal, and neonatal thyroid diseases requires an experienced multidisciplinary team including adult and pediatric endocrinologists, obstetricians, and sonographers.

6.
Article in Chinese | WPRIM | ID: wpr-885259

ABSTRACT

Objective:To explore the safety of cyclophosphamide combined with taxane chemotherapy in triple-negative breast cancer patients with chronic renal failure and the management strategy of complications.Methods:Data of 8 patients with triple-negative breast cancer and chronic renal failure admitted to our hospital from Jun 2016 to Dec 2019 were retrospectively analyzed.Results:Eight patients received standard cyclophosphamide combined with taxane (TC regimen) chemotherapy after operation, 5 of which received docetaxel 75 mg/m 2 + cyclophosphamide 600 mg/m 2, and 3 received albumin paclitaxel 260 mg/m 2+ cyclophosphamide 600 mg/m 2, during chemotherapy, only leukopenia, hair loss and gastrointestinal reactions occurred in grades 3 to 4, and the incidence was 25%, 25% and 12%, respectively. Four patients adjusted the dosage due to adverse reactions. One patient quit, the remaining patients successfully completed 4 cycles of chemotherapy. The average serum creatinine before chemotherapy was (498±63) μmol/L, and after chemotherapy, it was (518±61) μmol/L ( t=-2.335, P>0.05). Conclusions:Combined with chronic renal failure is not a contraindication to postoperative chemotherapy for patients with breast cancer. It is safe to choose standard TC regimen and adjust the dose of adjuvant chemotherapy for patients with triple-negative breast cancer.

7.
Article in Chinese | WPRIM | ID: wpr-879095

ABSTRACT

The aim of this paper was to explore the key genes and pathogenesis of ischemic stroke(IS) by bioinformatics, and predict the potential traditional Chinese medicines for IS. Based on the gene-chip raw data set of GSE22255 from National Center of Biotechnology Information(NCBI), the article enrolled in 20 patients with ischemic stroke and 20 sex-and age-matched controls, and differentially expressed genes(DEGs) were screened based on R language software. The DAVID tool and R language software were used to perform gene ontology(GO) biological process enrichment analysis and Kyoto encyclopedia of genes and gnomes(KEGG) pathway enrichment analysis. The DEGs were imported into STRING to construct a protein-protein interaction network, and the Molecular Complexity Module(MCODE) plug-in of Cytoscape software was used to visualize and analyze the key functional modules. Moreover, the core genes and the medical ontology information retrieval platform(Coremine Medical) were mapped to each other to screen the traditional Chinese medicines and construct drug-active ingredient-target network. Compared with healthy controls, 14 DEGs were obtained, of which 12 genes were up-regulated and 2 genes were down-regulated. DEGs were mainly involved in immune response, inflammatory process, signal transduction, and cell proliferation regulation. The interleukin-17(IL-17), nuclear factor kappaB(NF-κB), tumor necrosis factor(TNF), nucleotide binding oligomerization domain(NOD)-like receptor and other signaling pathways were involved in KEGG pathway enrichment analysis. The key modules of the DEGs-encoding protein interaction network mainly focused on 7 genes of TNF, JUN, recombinant immediate early response 3(IER3), recombinant early growth response protein 1(EGR1), prostaglandin-endoperoxide synthase 2(PTGS2), C-X-C motif chemokine ligand 8(CXCL8) and C-X-C motif chemokine ligand 2(CXCL2), which were involved in biological processes widely such as neuroinflammation and immunity. TNF and JUN were the key nodes in this module, which might become potential biological markers for diagnosis and prognosis evaluation of IS. The potential traditional Chinese medicines for the treatment of IS includes Salviae Miltiorrhizae Radix et Rhizoma, Croci Stigma, Scutellariae Radix, and Cannabis Fructus. The occurrence of stroke was the result of multiple factors. Dysregulation of genes and pathways related to immune regulation and inflammation may be the key link for the development of IS. This study provided research direction and theoretical basis for further exploring the mechanism of action of traditional Chinese medicine in the treatment of IS and searching for potential drug targets.


Subject(s)
Brain Ischemia , China , Computational Biology , Gene Expression Profiling , Humans , Ischemic Stroke , Medicine, Chinese Traditional , Stroke/genetics
8.
Article in Chinese | WPRIM | ID: wpr-879093

ABSTRACT

In this experiment, an ultra-high performance liquid chromatographytandem triple quadrupole mass spectrometry was established for the determination of caffeine in commercially available Ginkgo Folium. The samples were extracted by ultrasonic method with methanol, and separated on Waters CORTECS T3 column(2.1 mm×100 mm, 2.7 μm), with mobile phase of 0.1% formic acid solution-0.1% formic acid acetonitrile solution for gradient elution, at flow rate of 0.3 mL·min~(-1); column temperature of 30 ℃, and injection volume of 2 μL. Mass spectrometry was conducted at ESI~+ multiple reaction monitoring(MRM) mode; quantitative analysis was conducted with external standard method. The results showed that in the range of 0.099 6-9.96 ng·mL~(-1), there was a good linear relationship between the mass concentration of caffeine and the peak area, R~2=0.999; the average recovery was 84.51%, with RSD of 6.2%. The results of precision, repeatability and stability showed that the RSD was 5.1%, 5.9%, 7.2%, respectively. The content range of caffeine in 10 batches of Ginkgo Folium was 1.52-60.86 μg·kg~(-1). In conclusion, this method is accurate, reliable and reproducible, which provides a reference for the safety study of Ginkgo Folium.


Subject(s)
Caffeine , Chromatography, High Pressure Liquid , Ginkgo biloba , Tandem Mass Spectrometry
9.
Article in Chinese | WPRIM | ID: wpr-879059

ABSTRACT

To systematically evaluate the clinical efficacy and safety of Ginkgo Leaf Tablets(GLT) in the treatment of acute cerebral infarction(ACI). Seven databases both at home and abroad were systematically retrieved from their establishment to March 2020. The data of the included studies were extracted after review and screening. The quality of the included studies was assessed with the Cochrane risk bias assessment tool, and then the included studies were put into Meta-analysis by RevMan 5.3 to evaluate the total cli-nical efficiency, neurological function score, blood lipids and incidence of adverse reactions in treatment of ACI by GLT. Finally, the GRADE system was adopted to evaluate the evidence quality of each outcome indicator and form recommendations. Ten studies involving 886 participants were included, all of which were of low quality. Meta-analysis results showed that,(1)in terms of the total clinical efficiency, GLT+Western medicine was superior to Western medicine alone(RR_(NDS)=1.20, 95%CI[1.06, 1.36], P=0.005; RR_(NIHSS)=1.35, 95%CI[1.09, 1.69], P=0.007), and there was no statistical difference between GLT+Xuesaitong Injection+Wes-tern medicine and Xuesaitong Injection+Western medicine(RR=1.16, 95%CI[1.00, 1.35], P=0.05).(2)In terms of improving neurological function score, GLT+Western medicine was superior to Western medicine alone(MD_(NIHSS[moderate(severe)])=-1.55, 95%CI[-2.22,-0.88], P<0.000 01; MD_(NIHSS(severe))=-7.51, 95%CI[-8.00,-7.02], P<0.000 01; MD_(NDS)=-1.36, 95%CI[-2.39,-0.33], P=0.01), and GLT+Danshen Injection+Western medicine was superior to Danshen Injection+Western medicine(MD_(NDS)=-3.09, 95%CI[-3.84,-2.34], P<0.000 01).(3)In terms of regulating blood lipids, GLT+Western medicine was superior to Wes-tern medicine alone(MD_(TC)=-1.40, 95%CI[-2.13,-0.66], P=0.000 2; MD_(TG)=-1.29, 95%CI[-1.86,-0.73], P<0.000 01; MD_(LDL-C)=-1.48, 95%CI[-2.91,-0.04], P=0.04; MD_(HDL-C)=0.07, 95%CI[0.02, 0.12], P=0.009).(4)In terms of incidence of adverse reactions, there was no statistical difference between GLT+Western medicine and Western medicine alone(RR=0.63, 95%CI[0.30, 1.32], P=0.22). The results of the evaluation showed that the evidence level of each outcome indicator was low, and the recommendation was at weak level. In conclusion, GLT+Western medicine could improve the total clinical efficiency, neurological function score, and blood lipid status, with a low incidence of adverse reactions. However, due to the small amount of included stu-dies, low study quality and low level of evidence, it is expected to carry out clinical studies with standardized design and large sample size in the future to further investigate the clinical efficacy and safety of GLT in the treatment of ACI.


Subject(s)
Cerebral Infarction/drug therapy , Ginkgo biloba , Humans , Plant Leaves , Tablets , Treatment Outcome
10.
Article in English | WPRIM | ID: wpr-793015

ABSTRACT

Objective@#To compare the pathogenicity of isolates of sequence type 7 (ST-7) ( ) belonging to four different serogroups (A, B, C, and X).@*Methods@#Four ST-7 isolates serogrouped as A, B, C, and X and characterized by different capsule structures, were examined for their adhesion and invasion properties, and their ability to induce cytokine release and apoptosis in the host cell (the A549 cell line).@*Results@#Among the four ST-7 isolates, the serogroup A isolate possessed the strongest adhesion and invasion ability. This isolate also induced the release of the highest levels of the pro-inflammatory mediators interleukin-6, interleukin-1β, and interferon, and the highest apoptosis rate in the host cells. However, there was no significant difference in interleukin-8 and tumor necrosis factor-α secretion between the four isolates. Based on the findings, the serogroup X isolate had the weakest pathogenicity, whereas there was almost no difference in the pathogenicity of the isolates from serogroups B and C.@*Conclusions@#The differences in the capsular structure of the four isolates of ST-7 affected their pathogenic capacities. The findings also imply that the hyperinvasive ST-7 lineage may include hypoinvasive isolates.

11.
Mycobiology ; : 70-74, 2020.
Article in English | WPRIM | ID: wpr-902720

ABSTRACT

Sinowilsonia henryi is a rare and endangered plant, as well as an endemic species in China.In July 2018, leaf spot and blight disease was observed on S. henryi in Yichang, Hubei,China. A fungus isolated from disease tissues was identified as Gonatobotryum apiculatumbased on morphology and sequence analyses of ITS and LSU regions. Phylogenetic analysesindicated that the species belongs to Dothioraceae (Dothideales). Morphologically, the speciesproduced two distinct types of conidia from authentic media, both conidia weredescribed here. Pathogenicity tests showed that the fungus is a pathogen causing leaf spotson S. henryi. This is the first report of leaf spot and blight disease on S. henryi caused byG. apiculatum in China.

12.
Mycobiology ; : 70-74, 2020.
Article in English | WPRIM | ID: wpr-895016

ABSTRACT

Sinowilsonia henryi is a rare and endangered plant, as well as an endemic species in China.In July 2018, leaf spot and blight disease was observed on S. henryi in Yichang, Hubei,China. A fungus isolated from disease tissues was identified as Gonatobotryum apiculatumbased on morphology and sequence analyses of ITS and LSU regions. Phylogenetic analysesindicated that the species belongs to Dothioraceae (Dothideales). Morphologically, the speciesproduced two distinct types of conidia from authentic media, both conidia weredescribed here. Pathogenicity tests showed that the fungus is a pathogen causing leaf spotson S. henryi. This is the first report of leaf spot and blight disease on S. henryi caused byG. apiculatum in China.

13.
Article in Chinese | WPRIM | ID: wpr-865990

ABSTRACT

Objective:To explore the clinical teaching effect of case-based learning combined with diagnosis and treatment guidelines in standardized residency training in the department of rheumatology and immunology.Methods:Forty standardized trainees were randomized into two groups. One was the observation group, which adopted case-based learning combined with diagnosis and treatment guidelines. The other one was the control group, which performed the traditional clinical teaching mode. After 4 weeks, the assessment and satisfaction evaluation were carried out among the two groups. SPSS 19.0 was used for t test and Mann-Whitney test. Results:Students in observation group showed significantly better ability of physical examination and case analysis than those in the control group. The satisfaction degree of the students in the observation group was significantly higher in terms of diagnosis and treatment standard, clinical thinking, problem solving ability, self-learning ability and personal profession benefits than that in the control group.Conclusion:Case-based learning with diagnosis and treatment guidelines is an ideal teaching method combining theory with practice perfectly, which can significantly improve the effect of clinical teaching of standardized residency training in the department of rheumatology and immunology.

14.
Article in Chinese | WPRIM | ID: wpr-864802

ABSTRACT

Objective:To explore the real experience of parents caring for children with Kawasaki disease complicated with giant coronary artery aneurysm at home,in order to provide reference for targeted interventions.Methods:With phenomenological approaches in qualitative study,semi-structured interviews were conducted among 10 parents whose children with Kawasaki disease complicated with giant coronary artery aneurysm, and Colaizzi 's 7-step analysis method was used to analyze the data and extract the theme.Results:The care experience of parents were classified into four themes:continued negative emotions;lacked of family care knowledge;bore a huge burden of care; eager for support strength.Conclusions:Parents of children with Kawasaki disease complicated with giant coronary artery aneurysm have many difficulties in disease management,family care and other aspects in the process of caring for children.They also bear a greater burden of psychological pressure.Medical workers should pay attention to parent′s psychological status and related problems,and provide effective intervention measures to promote the rehabilitation of children and improve the quality of life of the family.

15.
Chinese Medical Journal ; (24): 2803-2807, 2020.
Article in English | WPRIM | ID: wpr-877935

ABSTRACT

BACKGROUND@#Allogeneic natural killer (NK) cell immunotherapy is recognized as a promising anti-tumor strategy, but whether it plays a role in poor CD4 recovery among human immunodeficiency virus type 1 (HIV-1) infected patients is unknown. This study aimed to investigate the safety and effectiveness of allogeneic NK cells immunotherapy on HIV-1 immunological non-responders (INRs) receiving antiretroviral therapy (ART).@*METHODS@#From February to April 2018, a prospective, randomized, controlled, open-label clinical trial, which enrolled 20 HIV-1 INRs following specific inclusion criteria, was conducted at Nankai University Second People's Hospital. Participants were randomly allocated (simple randomization 1:1) to either the combined treatment (NK + ART) group (n = 10) or the control (ART) group (n = 10). The allogenic highly activated NK cells from killer cell immunoglobulin-like receptor (KIR)/human leukocyte antigen (HLA)-Cw mismatched healthy donor were prepared (10 cells in each injection) and intravenously infused to each recruited patient of NK+ART group in three courses. Key immune parameters (CD4 count, CD8 count, CD4/CD8 ratio), laboratory tests (count of blood cells, biochemistry panel) and symptoms at baseline and at month 1, 3, 6, 9, 12, and 24 were measured/collected to analyze the safety and efficacy of the therapy. Comparisons were between the seven time-points of both groups using repeated measurement analysis of variance (ANOVA) test. Generalized estimating equations (GEE) model was performed to evaluate the overall effect of the NK+ART group vs. the ART group.@*RESULTS@#From baseline to 24 months, we noted a mean CD4 count augmentation (139 to 243 cells/μL) in the NK + ART group and (144 to 176 cells/μL) in the ART group (difference, 67; 95% CI, 10 to 124; P = 0.024). Our estimations revealed that NK+ART group could improve CD4 level (β = 54.59, P = 0.006) and CD8 level (β = 322.47, P = 0.010) on average among the six measurements compared with the ART group. Only two (2/10, 20%) participants in the NK+ART group developed a transient mild fever after the first course.@*CONCLUSIONS@#This preliminary study informs that HIV-1 INRs, allogenic NK cells immunotherapy is safe and could significantly improve CD4 recovery but not CD4/CD8 ratio. The practical effects, however, need long-term follow-up observations. Further study on the potential underlying mechanism is warranted. REGISTRATION INFO:: www.chictr.org.cn/showproj.aspx?proj=34912 (No. ChiCTR1900020634).


Subject(s)
CD4 Lymphocyte Count , HIV Infections/therapy , HIV-1 , Hematopoietic Stem Cell Transplantation , Humans , Immunotherapy , Killer Cells, Natural , Prospective Studies , Viral Load
16.
Article in Chinese | WPRIM | ID: wpr-872405

ABSTRACT

Establishment of an appraisal system in line with the Party building work of the new era, is imperative for implementation of the Party building responsibility system and furthering Party building quality. In September 2019, the authors customized a questionnaire and surveyed 629 in-job Party members of 10 tertiary public hospitals. By means of literature review, questionnaire surveys and expert interviews, they studied the current Party building appraisal system and existing problems. In addition, they probed into the significance of applying target management theory in the appraisal of Party building work at public hospitals, and applied such a theory in setting up a Party building appraisal system in line with the requirements of the Party in the new era, in such aspects as appraisal goal setting, management of the appraisal process, improvement of the appraisal system, and feedback of appraisal results.

17.
Chinese Journal of Dermatology ; (12): 914-916, 2020.
Article in Chinese | WPRIM | ID: wpr-870382

ABSTRACT

Objective:To analyze the difference in skin surface lipids (SSL) between facial skin lesions of neonates with acne and normal facial skin of healthy infants.Methods:SSL samples were collected from facial skin lesions of 36 neonates with acne (acne group) and normal facial skin of 36 healthy infants (control group) at the Department of Dermatology, Children′s Hospital, Capital Institute of Pediatrics from June 2018 to August 2018. A lipid map was constructed by ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-QTOF-MS), and specific information about lipid components was obtained by multivariate data analysis. Independent-sample t test was performed with SPSS19.0 software for comparisons between 2 groups. Results:Compared with the control group, the acne group showed significantly increased relative content of glycerolipids and prenol lipids on the facial skin surface (both P < 0.001), but significantly decreased relative content of fatty acids, glycerophospholipids, sphingolipids, sterol lipids, saccharolipids and polyketides ( P < 0.01 or 0.05) .Further analysis showed that the average relative content of total lipids, diglycerides, wax esters and squalene was significantly higher in the acne group than in the control group ( P < 0.001 or 0.05), but the average relative content of linoleic acid was significantly lower in the acne group than in the control group ( P < 0.001) . Conclusion:Compared with the facial skin of healthy infants, the facial skin lesions of neonates with acne showed significantly increased total lipid content and different lipid components.

18.
Article in Chinese | WPRIM | ID: wpr-869245

ABSTRACT

Objective:To analyze the blood glucose screening condition for individuals receiving physical examinations. Specifically, to examine the rates of abnormal postprandial glucose at different levels of fasting blood glucose and the characteristics of people who had normal fasting blood glucose with high postprandial glucose.Methods:Participants were individuals who received physical examinations at the Health Management Centre, Tianjin Medical University General Hospital, from January 2016 to December 2016. Data collection included general information, physical examination, and laboratory tests including fasting blood glucose, HbA1c, postprandial glucose, blood fat, and blood uric acid. The blood glucose indicators for the study population were analyzed. Among participants without diabetes, the rate of abnormal postprandial glucose at different fasting blood glucose levels or with a HbA1c screening were compared. For participants with complete information, chi-square tests and multivariate logistic regressions were used to analyze the characteristics of individuals who had normal fasting blood glucose with high postprandial glucose.Results:A total of 45 447 participants were included, with 23 001 males (50.61%) and 22 446 females (49.39%). For blood glucose, the most frequently completed indicator was fasting blood glucose (97.04%), then HbA1c (56.17%) followed by postprandial glucose (17.51%). There were 7 351 participants without diabetes who had all three glucose indicators. As fasting blood glucose increased, the abnormal rate of postprandial glucose increased gradually. When fasting blood glucose was lower than 5.6 mmol/L, the rate of abnormal postprandial glucose was 11.59%. When fasting blood glucose was between 5.6 mmol/L and 6.1 mmol/L, the rate was 33.9%. When fasting blood glucose was higher than 6.1 mmol/L, the rate was 68.73%. When the cutoff for fasting blood glucose was 5.6 mmol/L, the rate of abnormal postprandial glucose decreased compared with a cutoff of 6.1 mmol/L (11.59% vs. 14.32%, respectively). Combined with HbA1c screening, the rate of postprandial glucose abnormalities were both reduced (8.83% vs.11.59% for 5.6 mmol/L; 10.08% vs. 14.32% for 6.1 mmol/L). A total of 5 872 individuals had complete information and were included in the analysis. Participants who were men, >45 years old, and were overweight or obese had higher risk for abnormal postprandial glucose [ OR(95% CI): 2.85(2.33-3.48), 2.15(1.76-2.62), 1.82(1.45-2.27), and 2.64(2.04-3.42), respectively, P<0.05]. Hypertension, hyperlipidemia and hyperuricemia were also risk factors [ OR(95% CI): 1.80(1.51-2.15) and 1.52(1.27-1.82), respectively, P<0.05] Conclusions:The completion rate was highest for fasting blood glucose and lowest for postprandial glucose among the study population. The rate of abnormal postprandial glucose was high when fasting blood glucose was normal. Screening combined with HbA1c decreased the rate of postprandial glucose abnormalities. Postprandial glucose testing should be recommended for those who are male, older, overweight or obese, and have other risk factors including hypertension, hyperlipidemia, and hyperuricemia.

19.
Article in Chinese | WPRIM | ID: wpr-869192

ABSTRACT

Objective:To investigate the 18F-fluorodeoxyglucose (FDG) PET/CT imaging features of appendiceal mucinous neoplasms (AMN) and the diagnostic value of PET/CT for AMN. Methods:Sixteen patients (5 males, 11 females, age: (59.6±12.2) years) with AMN who underwent 18F-FDG PET/CT examination in the First Affiliated Hospital of Dalian Medical University between April 2013 and May 2019 were retrospectively analyzed. All AMN were divided into low-grade AMN (LAMN) group and mucinous appendiceal adenocarcinoma (MAA) group according to pathological results. The PET/CT imaging features were analyzed and compared between LAMN group and MAA group. The PET/CT imaging results were compared with histopathological results. Independent-sample t test was used for data analysis. Results:On PET/CT images, LAMN group showed shorter diameter ((3.31±0.74) vs (3.71±1.52) cm; t=-0.667, P<0.05) and lower cystic wall metabolic level (maximum standardized uptake value (SUV max): 2.73±1.31 vs 6.70±5.95; t=-1.844, P<0.05) than those of MAA group. AMN usually showed slightly high density fluid ((23.88±13.14) HU) in the appendiceal region, punctate calcification (10/16) and low to moderate metabolism of the cystic wall (SUV max: 4.71±4.64), and the pseudomyxoma peritonei incidence rate of AMN was about 7/16. Compared with the pathological results, PET/CT accurately diagnosed 11 cases of AMN, including 3 cases of AMN with pseudomyxoma peritonei, the sensitivity was 11/16, 3/7, respectively. PET/CT accurately diagnosed 7/9 patients with metastasis. Conclusion:18F-FDG PET/CT examination has good diagnostic value for AMN.

20.
Journal of Medical Postgraduates ; (12): 133-138, 2020.
Article in Chinese | WPRIM | ID: wpr-818389

ABSTRACT

Objectiveezrin gene is overexpressed in pancreatic cancer, and its upstream sequence plays an important role in gene expression. This study intends to knock out ezrin transcriptional regulatory region and identify its gRNA target sites for gene editing in pancreatic cancer cells.MethodsThe reporter gene expression vectors carrying the upstream segment of ezrin gene were transiently transfected into Panc-1 cells. The ezrin transcriptional regulatory regions were identified by double luciferase reporter gene detection system. Then, the online software was utilized to predict the gRNA target sites located at the upstream and downstream of ezrin transcriptional regulatory region. Two recombinant plasmids pX459-sgRNA-L and pX458-sgRNA-R contained these two sequences were constructed for gene editing. Moreover, in order to identify the targeted knockout of ezrin transcriptional regulatory region, the recombinant plasmids were co-transfected into Panc-1 cells, and the genome DNA contained gRNA target sites were amplified, subcloned and sequenced. Finally, Panc-1 cells transfected with recombinant plasmids were preliminary sorted using puromycin treatment. The cell proliferation was detected by water-soluble tetrazolium salt method.ResultsLuciferase data showed that ezrin gene fragment -1297/-1186 enhanced the transcriptional activity of SV40 promoter and ezrin promoter in Panc-1 cells. Subclonal sequencing data revealed that the recombinant plasmids carrying the gRNA target sequence of ezrin transcriptional regulatory region were co-transfected into Panc-1 cells could trigger the genomic DNA fragments, which located between gRNA-L and gRNA-R target sites. Cell proliferation assay showed that the proliferation was significantly inhibited after transfection.ConclusionThe targeted knockout of ezrin transcriptional regulatory region was achieved and the inhibition of Panc-1 cell proliferation may be related to this knockout.

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