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ObjectiveTo determine the epidemiological characteristics and risk factors of hypertension in residents in Songjiang District of Shanghai， and provide evidence for prevention and control of hypertension. MethodsLocal residents aged between 20 and 74 years were recruited from four towns in Songjiang District from 2016 to 2017 by using a multistage sampling strategy. Questionnaire surveys were conducted， and then physical examination， blood pressure measurement， glucose test and lipid test were performed. The data was analyzed by SPSS 16.0 software. Chi-square test was used to compare the differences in the prevalence of hypertension in residents， stratified by demographics and health conditions. Risk factors were determined by multivariate logistic regression. ResultsAmong 35 759 residents with a median age of 58 years and the proportion of male being 40.67%， crude prevalence of hypertension was 54.24% （19 395/35 759） and standardized prevalence was 31.69%. In the hypertensive patients， proportion of hypertension awareness was 61.09%， with 63.67% in male and 59.10% in female. Proportion of receiving treatment was 49.00%， with 50.91% in male and 47.51% in female. Furthermore， in the hypertensive patients with treatment， proportion of effectively controlling hypertension was 40.50%， with 44.46% in male and 37.21% in female. Multivariate logistic regression showed that male （OR=1.272）， senior age （40‒59 years old： OR=4.353， 60‒74 years old： OR=9.802）， overweight （OR=1.823）， obesity （OR=3.070）， central obesity （OR=1.380）， diabetes （OR=1.923）， dyslipidemia （OR=1.452）， and drinking （OR=1.338） were risk factors associated with hypertension. ConclusionPrevalence of hypertension is high， while the proportions of receiving treatment and effective control remain low in residents in Songjiang District. It warrants strengthening prevention and control of hypertension in targeted and young residents.
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OBJECTIVES@#To explore the value of three-dimensional fast gradient echo sequence (3D-GRE) in observation of the craniocervical junctional ligament.@*METHODS@#A total of 21 healthy volunteers underwent 3D-GRE imaging. The imaging data was imported into the post-processing workstation. The structures of the ligaments in the craniocervical junctional area were observed and evaluated by multiplanar reconstruction technique.@*RESULTS@#The features of ligaments in the craniocervical junction were shown clearly for all the 21 cases of volunteers. The scan time was 267-294 s. After the treatment with the three-dimensional reconstruction technique, the signal characteristics and the running structure of the transverse ligament, the alar ligament, the serrated ligament and the lamina could be effectively displayed.@*CONCLUSIONS@#The 3D-GRE can evaluate the three-dimensional data of craniocervical junctional ligament in a short period of time. Post-processing reconstruction technique can clearly evaluate the structure characteristics of each ligament, which can lay a foundation for further application in craniocerebral trauma patients.
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Diagnostic Imaging , Humans , Imaging, Three-Dimensional , Ligaments, Articular/diagnostic imaging , Magnetic Resonance ImagingABSTRACT
Objective:To investigate the effects of acupuncture-rehabilitation therapy on homocysteine (Hcy) and cognitive function in patients with cognitive impairment after ischemic stroke. Methods:From July, 2017 to June, 2018, 88 patients with cognitive impairment after ischemic stroke were randomly divided into group A (n = 29, cluster needling), group B (n = 30, cognitive training) and group C (n = 29, acupuncture-rehabilitation). Their serum Hcy content was measured and they were assessed with Montreal Cognitive Assessment (MoCA) before and 30 days after treatment. Results:The serum Hcy content was the least in group C after treatment (F = 6.570, P < 0.01), with the most score of MoCA (F = 31.526, P < 0.001). Conclusion:Acupuncture-rehabilitation therapy is more effective than simple cluster needling or cognitive training for patients with cognitive impairment after ischemic stroke.
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Objective@#To survey the current situation of wearing protective articles by accompanying examiners in the nursing conditions of intervention and non-intervention during CT diagnosis in a tertiary A hospital.@*Methods@#A control group and an intervention group were set up to investigate the situation of wearing protective devices. Attempt was made to use mobile lead screens in place of personal protective devices and their effects were investigated.@*Results@#A total of 4 890 unavoidable accompanying examiners wearing protective equipment during CT examination were investigated. After nursing guidance, the wearing rate increased from 73% to 94%, and the complete wearing rate increased from 19% to 81%. Refusal to wear protective devices was mostly due to limited time. Refusal rate of emergency accompanying examiners was significantly higher than that of outpatient and inpatient accompanying examiners. After using mobile lead screen, the probability of personnel protection increased to 99%.@*Conclusions@#Nursing intervention can effectively improve the wearing rate and complete wearing rate of protective articles for accompanying examiners. Moving glass lead screen is conducive to the improvement of protection level for accompanying examiners..
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Objective@#To assess the interobserver variations in delineating the planning target volume (PTV) and organs at risk (OAR) using different contouring methods during intensity-modulated radiation therapy (IMRT) for nasopharyngeal carcinoma (NPC), aiming to provide references for the quality control of multi-center clinical trials.@*Methods@#The PTV and OAR of CT image of 1 NPC patient manually delineated by 10 physicians from 8 different radiation centers were defined as the " manual contour group" , and the OAR auto-contoured using the ABAS software and modified by the physicians were defined as the " auto+ manual contour group" . The maximum/minimum ratio (MMR) of the PTV and OAR volumes, and the coefficient of variation (CV) for different delineated contours were comparatively evaluated.@*Results@#Large variation was observed in the PTV and OAR volumes in the manual contour group. The MMR and CV of the PTV were 1.72-3.41 and 0.16-0.39, with the most significant variation in the PTVnd (MMR=3.41 and CV=0.39 for the PTVnd-L). The MMR and CV of the manually contoured OAR were 1.30-7.89 and 0.07-0.67. The MMR of the temporal lobe, spinal cord, temporomandibular joint, optic nerve and pituitary gland exceeded 2.0. Compared with the manual contour group, the average contouring time in the auto+ manual group was shortened by 68% and the interobserver variation of the OAR volume was reduced with an MMR of 1.04-2.44 and CV of 0.01-0.37.@*Conclusions@#Large variation may occur in the PTV and OAR contours during IMRT plans for NPC delineated by different clinicians from multiple medical centers. Auto-contouring+ manually modification can reduce the interobserver variation of OAR delineation, whereas the variation in the delineation of small organs remains above 1.5 times. The consistency of the PTV and OAR delineation and the possible impact upon clinical outcomes should be reviewed and evaluated in multi-center clinical trials.
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Objective To assess the interobserver variations in delineating the planning target volume (PTV) and organs at risk (OAR) using different contouring methods during intensity-modulated radiation therapy (IMRT) for nasopharyngeal carcinoma (NPC),aiming to provide references for the quality control of multi-center clinical trials.Methods The PTV and OAR of CT image of 1 NPC patient manually delineated by 10 physicians from 8 different radiation centers were defined as the "manual contour group",and the OAR auto-contoured using the ABAS software and modified by the physicians were defined as the "auto+manual contour group".The maximum/minimum ratio (MMR) of the PTV and OAR volumes,and the coefficient of variation (CV) for different delineated contours were comparatively evaluated.Results Large variation was observed in the PTV and OAR volumes in the manual contour group.The MMR and CV of the PTV were 1.72-3.41 and 0.16-0.39,with the most significant variation in the PTVnd (MMR=3.41 and CV =0.39 for the PTVnd-L).The MMR and CV of the manually contoured OAR were 1.30-7.89 and 0.07-0.67.The MMR of the temporal lobe,spinal cord,temporomandibular joint,optic nerve and pituitary gland exceeded 2.0.Compared with the manual contour group,the average contouring time in the auto+ manual group was shortened by 68％ and the interobserver variation of the OAR volume was reduced with an MMR of 1.04-2.44 and CV of 0.01-0.37.Conclusions Large variation may occur in the PTV and OAR contours during IMRT plans for NPC delineated by different clinicians from multiple medical centers.Auto-contouring+ manually modification can reduce the interobserver variation of OAR delineation,whereas the variation in the delineation of small organs remains above 1.5 times.The consistency of the PTV and OAR delineation and the possible impact upon clinical outcomes should be reviewed and evaluated in multi-center clinical trials.
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Objective To survey the current situation of wearing protective articles by accompanying examiners in the nursing conditions of intervention and non-intervention during CT diagnosis in a tertiary A hospital. Methods A control group and an intervention group were set up to investigate the situation of wearing protective devices. Attempt was made to use mobile lead screens in place of personal protective devices and their effects were investigated. Results A total of 4890 unavoidable accompanying examiners wearing protective equipment during CT examination were investigated. After nursing guidance, the wearing rate increased from 73％ to 94％, and the complete wearing rate increased from 19％ to 81％. Refusal to wear protective devices was mostly due to limited time. Refusal rate of emergency accompanying examiners was significantly higher than that of outpatient and inpatient accompanying examiners. After using mobile lead screen, the probability of personnel protection increased to 99％. Conclusions Nursing intervention can effectively improve the wearing rate and complete wearing rate of protective articles for accompanying examiners. Moving glass lead screen is conducive to the improvement of protection level for accompanying examiners..
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Objective To study on the procedure, safety and effectiveness of polyetheretherketone (PEEK) implant applied to cranioplasty of skull defect. Methods A total of 11 cases (10 male, 1 female) of unilateral skull defect, more than 6 months post operation, were included in this study. PEEK implant was custom-made by three-dimensional numerically controlled processing depended on the data obtained from 1 mm-slice CT scan before cranioplasty individually. Autoclaved implants were applied to cranioplastic surgeries under general anesthesia. Findings of imaging examination and vital signs were compared before and after operation. Vital sign changes and circumstances during procedure were noted, and following-up reviews were performed on 2-week, 3-month and 1 year after operation respectively. Results Wounds healing were uneventful in 11 cases, no postoperative paresthesia presented. Surgical complications including temporary subcutaneous exudates were cleared up by puncture and aspiration in 5 cases, subcutaneous hematoma duo to drainage removal in 1 case, of which an emergency evacuation was performed and the implant was still with instant incision closure and then smooth recovery eventually. No significant abnormal fluctuation of laboratory examination was reported, moreover no artifact interference was found on postoperative CT scan or MR image. The subjective feelings and external sensory effects are satisfactory in patients. No emerging dysfunction of central nervous system or other organs was found, and no long-term complication was appeared. Conclusion It is feasible and safe to apply PEEK implant to cranioplasty without additional operative difficulty. This kind of material is an ideal alternative for repairing skull defect to patients with good financial condition and specific demands for postoperative status especially.
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OBJECTIVE:To investigate the inhibitory effect of Baixuan xiatare tablet on the model mouse with allergic contact dermatitis (ACD). METHODS:60 BALB/c mice were equally randomized into normal control (isometric solvent) group,model (isometric solvent)group,ebastine(positive control,0.003 g/kg)group and the groups of high,middle and low doses of Baixuan xiatare tablet(2.0,1.0 and 0.5 g/kg). The mice were given drugs,ig,once daily for 14 consecutive days. 0.5% 2,4-dinitrofluoro-benzene(DNFB)acetone olive oil solution was applied,for sensitization,on the prepared mouse’s skins one and two days before administration,and 0.2% DNFB acetone olive oil solution on their left ears 16 days thereafter to establish mouse models of ACD. At 48 h after successful establishment of the models,the thickness of the mouse’s left ear margin was measured and the difference value and swelling degree were calculated;flow cytometer was used to determine the levels of T lymphocyte subsets CD4+ and CD8+ in mouse blood and calculate the ratio of CD4+ to CD8+;the levels of interleukin 4(IL-4)and IL-6 in mouse serum were de-termined. RESULTS:Compared with normal control group,those in the model group had higher difference value of ear margin and swelling degree,lower level of CD4+ in blood and ratio of CD4+ to CD8+,and higher content of IL-6 in serum. There was statisti-cally difference (P<0.01). Compared with model group,those in the groups of high,middle and low doses of Baixuan xiatare tablet had lower degree of left ear swelling and higher level of CD4+ in blood;those in the groups of high and middle doses thereof had lower difference value of left ear margin and level of IL-6 in serum;and those in the group of high dose thereof had higher lev-el of CD8+ in blood. There was statistically significance(P<0.01 or P<0.05). CONCLUSIONS:Baixuan xiatare tablet has inhibi-tory effect to some degree on the mouse model with ACD by a mechanism which may be related to the balance of subsets CD4+and CD8+in blood and the reduction of IL-6 in serum.
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Background: The relationship between Helicobacter pylori (Hp) infection and the risk of esophageal squamous cell carcinoma is unclear. Aim: The purpose was to investigate the effects of CagA+ Hp on esophageal squamous carcinoma Ec 109 cells in vitro and explore the molecular mechanisms underlying these effects. Materials and Methods: Ec 109 cells were treated with CagA+ Hp fi ltrate at a concentration of 1.0 mg/mL or 50 μg/mL in vitro, proliferation and apoptosis of Ec 109 cells were assayed, the secretion of IL-8 was measured by ELISA, and the levels of Src homology-2 domain-containing phosphatase (SHP-2) mRNAs was assayed by RT-PCR.. Furthermore, after pretreatment of Ec109 cells with the specifi c p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580, the p38 pathway was detected. Results: CagA+ Hp fi ltrate enhanced both the proliferation and apoptosis of Ec 109 cells. In addition, cytokine IL-8 release was signifi cantly increased, and the expression of SHP-2 mRNA declined sharply in the CagA+ Hp group. Furthermore, after pretreatment of Ec109 cells with the specifi c p38 MAPK inhibitor SB203580, Ec109 cells proliferation and IL-8 secretion were inhibited. Conclusions: Our results suggest that CagA+ Hp fi ltrates could induce proliferation and the secretion of IL-8 by Ec109 cells in vitro. IL-8 secretion was induced through the activation of the p38 MAPK signal pathway.
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Objective To explore differences of ultrasoundgraphy and CT guided percutaneous paracentesis for liver abscess and provide references for the paracentesis treating of liver abscess .Methods 68 patients with liver abscess arranged ultrasoundgraphy guided percutaneous paracentesis were regarded as ultrasound group ,and 38 patients accepted CT guided percutaneous paracentesis were considered as CT group .The succeed rate of paracentesis ,operation period ,treatment effectiveness ,complications of the 2 groups were compared .Results The succeed rate of paracentesis in ultrasound group (81 .7% ) was not different obviously to CT group(82 .6% ) (χ2 =0 .016 2 ,P=0 .898 6) .The operation period of ultrasound group(22 .3 ± 5 .6)min was shorten than CT group (25 .6 ± 4 .8)min ,t= 3 .057 4 ,P< 0 .05 .The heal rate of the 2 groups(91 .2% ,92 .1% ) was not different (χ2 = 0 .027 1 ,P=0 .869 3) .There was no different of the complications in the 2 groups(5 .88% ,8 .57% )(χ2 =0 .010 1 ,P=0 .920 1) .Conclusion Either ultrasoundgraphy or CT guided percutaneous paracentes is feasible and safe for liver abscess .Compared to CT ,the ultra-soundgraphy is more convenient and economic .
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Objective To compare the long-term efficacy between two radiochemotherapy regimens for locally advanced nasopharyngeal carcinoma (NPC):intensity-modulated radiotherapy with concurrent chemotherapy (CCRT) versus neoadjuvant chemotherapy (NACT) followed by CCRT.Methods A retrospective analysis was performed on the clinical data of 278 patients with locally advanced NPC who were admitted to our hospital from 2001 to 2008.Of the 278 patients,133 received CCRT,and 145 received NACT followed by CCRT (NACT + CCRT).Results The follow-up rate was 96.6％.The 5-year overall survival (OS),distant metastasis-free survival (DMFS),recurrence-free survival (RFS),and progression-free survival (PFS) were 78.1％,78.0％,90.6％,and 72.0％,respectively.There were no significant differences between the CCRT group and NACT + CCRT group in 5-year OS (79.9％ vs.76.4％,P =0.443),DMFS (77.1％ vs.78.9％,P=0.972),RFS (91.6％ vs.89.8％,P=0.475),and PFS (71.6％ vs.72.2％,P=0.731).Subgroup analysis showed that compared with CCRT,NACT + CCRT did not significantly improve 5-year RFS in T3-4N0-1 patients (90.7％ vs.86.9％,P=0.376) and did not significantly improve 5-year DMFS in patients with advanced N-stage disease (57.6％ vs.69.7％,P =0.275).There were significantly higher numbers of individuals with neutropenia,decrease in hemoglobin,and upper gastrointestinal reactions in patients treated with NACT + CCRT than in those treated with CCRT (100 vs.52,P=0.000;64 vs.35,P=0.010;90 vs.63,P=0.044).Conclusions Compared with CCRT,NACT + CCRT does not significantly improve the prognosis in patients with locally advanced NPC and leads to significant increases in grade ≥ 3 toxicities (neutropenia,decrease in hemoglobin,and upper gastrointestinal reactions).The role of NACT in the treatment of locally advanced NPC needs further study
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OBJECTIVE@#To determine the value of high-resolution three dimensional contrast enhanced magnetic resonance venography (3D CE-MRV) in evaluating sinus meningiomas in the region of interest (ROI).@*METHODS@#Twenty patients with sinus meningiomas underwent 3D CE-MRV with ROI preoperatively (including 9 patients postoperatively). We observed the changes of venous sinus adjacent the tumor.@*RESULTS@#All patients received high-resolution image, the single acquisition time was about 11.4 s, and the voxel value was about 1.3 mm3. The images of 20 patients showed the change of the sinus clearly, 6 of which with integral sinus, 14 with sinus invaded at various degrees, including 5 with sinus occlusion and 9 with stenosis. CE-MRV also showed 4 patients with clear sinuses, 1 with narrow sinus, partial interruption, and 4 with sinus removed after the surgery.@*CONCLUSION@#Application of high-resolution 3D CE-MRV in ROI in sinus meningiomas may help obtain a series of high-resolution images in a short time, show the relationship between the tumor and venous sinus, display the degree of invasion of venous sinus clearly, provide information for the surgical treatment, and evaluate the change of sinus after the surgery.
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Adult , Aged , Aged, 80 and over , Cranial Sinuses , Pathology , Female , Humans , Image Enhancement , Methods , Imaging, Three-Dimensional , Magnetic Resonance Angiography , Methods , Male , Meningeal Neoplasms , Diagnosis , Meningioma , Diagnosis , Middle Aged , Phlebography , MethodsABSTRACT
Objective To study the preoperative application value of the three-dimensional angiography in surgical strategy for the elderly patients with meningioma. Methods Forty-two elderly patients aged 60 years and over with meningioma were examined preoperatively with multi-slice spiral CT angiography(MSCTA) and (or) 3 dimensional contrast-enhancement MR angiography(3D CE-MRA)as simulation group.In three-dimensional post-processing workstation,the oppression and invasion degree of the intracranial important blood vessels,nervous running zone and sinus,as well as surgical risks were evaluated. 28 elderly patients without the above examination were as control group. The operation time, blood transfusions and postoperative complications were compared between the two groups. Results All 42 cases of simulation group completed reasonable surgical plan and approach.The incidence of postoperative complications were lower in simulation group than in control group (35.7％ vs.50.0％,P＞0.05),intra-operative blood transfusion were significantly decreased in simulation group as compared with control group [(5301.0± 150.6) ml vs.( 621.4±226.7)ml,t=2.01,P=0.049],operation time after three-dimensional simulation were reduced as compared with control group [(257.1 ±72.6) min vs.(307.5±88.2)min,t=2.61,P=0.011].Conclusions Application of three-dimensional angiography in elderly patients with meningioma to evaluate the operative risk may help make reasonable surgical strategy,thus reducing the surgical trauma and complications.
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Many laboratories have been attempting to integrate Chinese medicine (CM) with the research of stem cells in order to explore this promising frontier. Studies on the combination of CM and bone marrow-derived mesenchymal stem cells (BMSCs) have found that some effective components from CM could activate endogenous stem cells and induce stem cells to differentiate into neural-like cells in vitro and promote angiogenesis. This review summarized the latest research findings of BMSCs and their application combined with CM in the treatment of cerebral ischemia.
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Bone Marrow Cells , Cell Biology , Brain Ischemia , Drug Therapy , Therapeutics , Combined Modality Therapy , Drugs, Chinese Herbal , Therapeutic Uses , Humans , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Yinian Jiangya Decoction (YNJYD) on cytokine secretion in spontaneoulsy hypertensive rats (SHRs) vascular endothelium.</p><p><b>METHODS</b>Aortic endothelial cells (ECs) were primarily cultured from SHRs; male SD rats were treated with different doses (high, medium, and low doses) of YNJYD, the blood was collected on the 21st day, and then, the serum was separated. ECs were cocultured with the serum for different time courses, and the culture supernatant concentrations of endothelin (ET)-1, nitric oxide (NO), tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitor (PAI-1) were determined by ABC-ELISA methods.</p><p><b>RESULTS</b>ET-1, NO, t-PA, and PAI-1 levels in endothelial cell culture supernatant were increased in a time-dependent manner; YNJYD could significantly elevate NO and t-PA expressions in ECs, while ET-1 and PAI-1 expressions were dramatically decreased; these effects of YNJYD were in a concentration-dependent manner.</p><p><b>CONCLUSION</b>The therapeutic effect of YNJYD on hypertension is attributed to its effect on regulating vessel dilation and blood coagulation, in which ET-1/NO and PAI-1/t-PA are two pairs of pivotal mediators.</p>
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Animals , Cytokines , Bodily Secretions , Drugs, Chinese Herbal , Pharmacology , Endothelial Cells , Metabolism , Endothelin-1 , Metabolism , Endothelium, Vascular , Bodily Secretions , Male , Nitric Oxide , Metabolism , Plasminogen Activator Inhibitor 1 , Metabolism , Rats , Rats, Inbred SHR , Subcellular Fractions , Metabolism , Time Factors , Tissue Plasminogen Activator , MetabolismABSTRACT
Objective To probe the value of multi-slice spiral CT angiography (MSCTA) in decreasing surgical trauma of the cranial base meningioma. Methods Thirty-two patients with cranial base meningioma were examined preoperatively with MSCTA to observe the shape and the relationship with the adjacent vessels and the skull base. Three-dimensional images were reconstructed to imitate the approach of operation and compared with surgical findings. Meanwhile, 22 patients withnot MSCTA were selected randomly as control group. The amount of blood transfusion and the occurrence rate of complications were compared between the two groups. Results MSCTA depiceted clear three dimensional images of the meningioma and the relationship with the adjacent vessels and the skull base, corresponded very well to the surgery. By imitating the operation, all patients were designed the incision size of bone appropriately, the vessels of peritumoral were kept off effectively and the risk of the embedded vascular were assessed accurately. The conventional surgical approach and method were changed in 9 patients, 4 formulated the planning of the sub-total resection and radiotherapy preoperatively. Compared with control group, the amount of blood transfusion reduced significantly (P<0.05) and postoperative complications decreased. Conclusion MSCTA can imitate the surgical operations in multi-angle and supply the vital information for choosing the proper surgical approach, thereby reducing surgical trauma and postoperative complications.
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This project aimed to investigate the effect of taurine on cell cycle regulatory protein p27, Cyclin D1 and nuclear factor-kappa B (NF-kappaB) p65 in the proliferation of cultured neonatal rat cardiac fibroblast (CFb) induced by angiotensin II (Ang II), and to explore the effect of taurine on the signal transduction pathway in CFb proliferation. The cultured neonatal rat CFbs were isolated by trypsin digestion method. The proliferation of CFb was induced by Ang II and detected with thiazole blue (MTT) colorimetric assay. The protein expression of p-PKCalpha in cells was determined with Western blotting technology. The expression of p27 was analyzed by flow cytometry. The expression of Cyclin D1 was determined with the combination of immunocytochemical staining and image analysis software. The nuclear translocation of NF-kappaB p65 was determined with immunofluorescence staining. Among the concentrations ranged from 40 to 160 mmol L(-1), taurine significantly inhibited p-PKCalpha expression. Taurine increased p27 expression and inhibited the nuclear translocation of NF-kappaB p65 in CFb (P < 0.05, P < 0.01, respectively) by inhibition of p-PKCalpha expression. And PKC inhibitor (Che) could improve the inhibitory action of taurine on CFb proliferation. The effects of taurine on CFb proliferation might be due to inhibition of p-PKCalpha expression and p27 expression increase and the nuclear translocation of NF-kappaB p65 inhibition followed.
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Animals , Cell Proliferation , Cells, Cultured , Cyclin D1 , Metabolism , Cyclin-Dependent Kinase Inhibitor p27 , Metabolism , Fibroblasts , Cell Biology , Myocytes, Cardiac , Cell Biology , Protein Kinase C-alpha , Metabolism , Rats , Rats, Wistar , Signal Transduction , Taurine , Pharmacology , Transcription Factor RelA , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To establish a method for analyzing amorphous organic compound components of space Platycodon grandflorum rapidly.</p><p><b>METHOD</b>FTIR was applied for measuring and comparing P. grandflorum of comparison group,the ground group and the 4" generation of space group.</p><p><b>RESULT</b>Several active components (platycodin, polysaccharide etc.) contents of space group are increased obviously.</p><p><b>CONCLUSION</b>The major components and the structures remained inextenso,and the effictive component contents are enhanced in the space P. grandiflorum. FTIR is a fast method to analyze the changes of amorphous organic compound components of the space traditional Chinese medicines.</p>
Subject(s)
Mutagenesis , Physiology , Platycodon , Chemistry , Polysaccharides , Chemistry , Saponins , Chemistry , Space Flight , Spectroscopy, Fourier Transform Infrared , Methods , WeightlessnessABSTRACT
Rapidly activating component of delayed rectifier potassium current (I(Kr)) plays a key role in the repolarization phase of cardiac action potential. Human ether-a-go-go-related gene (HERG) encodes the alpha subunit of this potassium channel. Mutations of HERG gene induce genetic long QT syndrome (LQTS). Furthermore, I(Kr)/HERG is the target of some drugs which may cause cardiac QT interval prolongation. Some other drugs with different chemical structures also may block the channel and prolong QT interval, which even developed into acquired arrhythmias. This review summarized the recent progress of structure, gating mechanisms and functions of I(Kr)/HERG channel, I(Kr)/HERG related arrhythmias, interaction between K+ channel and drugs, and strategies of grading-up the I(Kr)/HERG target.