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1.
China Pharmacy ; (12): 1003-1008, 2021.
Article in Chinese | WPRIM | ID: wpr-876273

ABSTRACT

OBJECTIVE:To study influential factors for medication compliance of phosphate binder in patients with maintenance hemodialysis and the effects of pharmacist intervention ,and to improve medication compliance and the effects of disease control. METHODS :The patients with maintenance hemodialysis who were treated in the blood purification center of our hospital from Jun. to Dec. ,2019 were selected for questionnaire survey. The questionnaires involved general information , medication compliance of phosphate binder ,disease and medicine related knowledge ,social support ,self-efficacy. The t-test,χ2 test and multivariate Logistic regression analysis were used to analyze influential factors for medication compliance. The patients were randomly divided into pharmaceutical intervention group and non-intervention group. Intervention group were provided with pharmaceutical care for 3 months according to risk factors. Blood phosphorus level and medication compliance was compared between 2 groups. RESULTS :Totally 298 patients completed the survey (effective recovery rate of 96.1%). Among them ,163 patients(54.7%)had good adherence to phosphate binder ,while 135 patients(45.3%)had poor compliance. Results of single factor analysis showed that medication compliance of phosphate binder was closely associated with age ,dialysis duration , parathyroid hormone levels ,total daily dose ,daily dose of phosphate binder ,disease and medicine related knowledge scores , social support ,self-efficacy(P<0.05). Results of multivariate Logistic regression analysis showed that total daily dose ,daily dose of phosphate binder ,disease and medicine related knowledge scores ,social support and self-efficacy were the influential factors for medication compliance (P<0.05 or P<0.01). Medication compliance ,disease control status ,disease and medicine related knowledge score , social support and self-efficacy in pharmaceutical intervention group were significant improved , blood phosphorus level was significant lower ,compared with non-intervention group (P<0.05). CONCLUSIONS :Independent risk factors influencing medication compliance of phosphate binder include total daily dose ,daily dose of phosphate binder ,disease and medicine related knowledge scores ,social support and self-efficacy. The patients with maintenance hemodialysis have poor compliance to phosphate binder. Pharmacists should take individualized and targeted intervention measures for the above risk factors,which can effectively improve the medication compliance and disease prognosis of patients.

2.
Article in Chinese | WPRIM | ID: wpr-910878

ABSTRACT

Objective:To explore the clinical value of heparin binding protein (HBP) in bronchoalveolar lavage fluid (BALF) for diagnosis and differential diagnosis of bacterial pneumonia.Methods:Eighty eight patients with pulmonary infection from the respiratory department of the Fourth Affiliated Hospital of Anhui Medical University from January 2019 to January 2021 were enrolled in the study, including 48 cases of bacterial pneumonia and 40 cases of non-bacterial pneumonia; meanwhile, 40 non-pulmonary infection patients were also enrolled as the control group. The BALF levels of HBP, procalcitonin (PCT), interleukin-6 (IL-6) were measured, and the clinical values of the above indexes in differential diagnosis of bacterial and non-bacterial pneumonia were analyzed.Results:The BALF levels of HBP and IL-6 in bacterial pneumonia group were significantly higher than those of the non-bacterial pneumonia group and the control group ( P<0.05). ROC curve showed that the areas under the curve (AUC) of HBP and IL-6 were 0.930 and 0.893 for the early diagnosis of bacterial pneumonia; and the sensitivity was 88.5% and 82.7%, the specificity was 92.5% and 92.5%, respectively. Combined detection of HBP and IL-6, the AUC was 0.942 and the sensitivity was 94.2% and the specificity was 95.0%. When they were used to distinguish bacterial pneumonia, the AUC of HBP and IL-6 were 0.890 and 0.777, and the sensitivities were 80.8% and 71.2%, and the specificity were 91.7% and 75.0%, respectively. Combined detection of HBP and IL-6, the AUC was 0.902, and the sensitivity was 96.2% and the specificity was 79.2%. Conclusions:BALF HBP and IL-6 have good clinical value in the early diagnosis and distinguishing bacterial pulmonary infection and the joint value of the two is better.

3.
Article in Chinese | WPRIM | ID: wpr-908227

ABSTRACT

Objective:To investigate the effect of attention and interpretation therapy on sleep dysfunction and quality of life in perimenopausal syndrome patients.Methods:From August 2018 to January 2020, a total of 76 patients with perimenopausal syndrome were divided into experimental group (38 cases) and control group (38 cases) according to the random number table method. Two groups recieved routine therapy and nursing care, on the basis of this, the experimental group was given attention and interpretation therapy for 10 weeks. Before intervention and after 10 weeks of intervention, the effects were assessed by Pittsburgh Sleep Quality Index (PSQI) and Menopause-Specific Quality of Life (MENQOL), respectively.Results:There was no significant difference in the score of PSQI, MENQOL before intervention between the two groups ( P>0.05). After intervention, the sleep quality, sleep duration, sleep efficiency scores and total PSQI scores were 0.79±0.10, 1.48±0.23, 1.11±0.22, 9.70±0.59; in addition, the vasomotor symptoms, psychological symptoms, somatic symptoms and total MENQOL scores were 3.06±0.81, 2.06±0.81, 2.50±0.51, 2.63±0.39 in the experimental group, significanlty lower than those in the control group (1.03±0.22, 1.85±0.33, 1.25±0.28, 10.59±0.66, 3.69±0.95, 2.83±0.77, 2.92±0.94, 3.18±0.53), the differences were statistically significant ( t values were 2.306-6.021, P<0.05). Conclusions:Attention and interpretation therapy can effectively alliviate sleep dysfunction and improve quality of life of perimenopausal syndrome patients.

4.
Article in Chinese | WPRIM | ID: wpr-879544

ABSTRACT

OBJECTIVE@#To explore the genetic basis for Chinese pedigree affected with tuberous sclerosis complex (TSC).@*METHODS@#The proband and his family members were subjected to Sanger sequencing for variants of the TSC1 and TSC2 genes.@*RESULTS@#The proband was found to harbor a c.2837+1dupG splicing variant at a donor site of the TSC2 gene. The same variant was not found among his family members and the fetus during his mother's subsequent pregnancy.@*CONCLUSION@#The c.2837+1dupG splicing variant of the TSC2 gene has probably predisposed to the TSC in this pedigree. Above finding has enriched the spectrum of pathogenic variants associated with this disease.


Subject(s)
Female , Genetic Testing , Humans , Male , Mutation , Pedigree , Pregnancy , Prenatal Diagnosis , Tuberous Sclerosis/genetics , Tuberous Sclerosis Complex 2 Protein/genetics
5.
China Pharmacy ; (12): 234-238, 2020.
Article in Chinese | WPRIM | ID: wpr-817368

ABSTRACT

OBJECTIVE:To provide reference for individualized treatment of patients with Neuroleptics-induced malignant syndrome(NMS). METHODS :A patient with NMS related to antipsychotics was admitted to our hospital in Sept. 19th 2018. Clinical pharmacists provided pharmaceutical care throughout the whole process ,and put forward suggestions for medication. Through literature review ,clinical pharmacists summarized the clinical manifestations ,risk factors ,pathogenesis,diagnosis and therapeutic drugs of NMS. RESULTS & CONCLUSIONS :Based on the history of antipsychotic drug use ,the characteristic clinical manifestations of NMS and laboratory examination ,the clinical pharmacist proposed that the patient suffered from antipsychotic drug-related NMS ,and the doctor adopted the suggestions. In the course of treatment ,the clinical pharmacist suggested that the subhibernating mixture should be stopped ;Bromocriptine mesylate tablets should be used in combination with continuous hypothermia instrument for physical cooling ,and the treatment course should be at least 10 days according to drug use before admission and medication plan after admission. The doctor adopted the suggestion. The symptoms began to relieve on the third day ,and the symptoms basically disappeared on the 10th day ,then the patient was discharged on the 13th day. The clinical manifestations of NMS were high fever ,myotonia,mental state change ,autonomic nervous disorder ,creatine phosphokinase and leukocyte increase etc. ;risk factors included drug factors ,demographic factors ,genetic and etc. ;the pathogenesis may be associated with dopaminergic receptor block and musculoskeletal fiber toxicity ;the identification diagnosis was based on clinical manifestation,including the onset time ,neuromuscalar reactivity ,remission time ,etc.;the commonly used drugs were bromocriptine mesylate and dantraline.

6.
Article in English | WPRIM | ID: wpr-827782

ABSTRACT

A series of novel pyrano[2, 3-d]trizaole compounds were synthesized and their α-glucosidase inhibitory activities were evaluated by in vitro enzyme assay. The experimental data demonstrated that compound 10f showed up to 10-fold higher inhibition (IC74.0 ± 1.3 μmol·L) than acarbose. The molecular docking revealed that compound 10f could bind to α-glucosidase via the hydrophobic, π-π stacking, and hydrogen bonding interactions. The results may benefit further structural modifications to find new and potent α-glucosidase inhibitors.

7.
Article in Chinese | WPRIM | ID: wpr-846051

ABSTRACT

Objective: To evaluate the synergistic anticancer effects of the combination of apigenin (Api) and tanshinone Ⅱ A (Tan IIA), and investigate the mechanisms of pharmacological effects and their potential applications as an anticancer therapy in clinics. Methods: MTT assay were used to determine anticancer effects of the combination of Api and Tan ⅡA on BGC823, MCF7, and SMMC7721 cells. AV-PI dual stain and PI staining method were used for detecting the effect of the two drugs combination on BGC823 cell apoptosis and cell cycle. Expression of p53, BAX/BCL-2, cyclin B1 and D1 proteins were determined by Western blotting. Circular dichroism method and DNA melting point method were explored to detect interaction among the two drugs and DNA. S180 tumor xenograft mice model was used to evaluate the antitumor effects of the two drugs combination. Results: Tan IIA combined with Api exerted synergistic inhibitory effects on the proliferation of BGC823 and other tumor cells with the CI of 0.28. After tumor cell treated by combination of Tan IIA and Api, the tumor cell apoptosis was significantly enhanced and the value of BAX/BCL-2 in cells was up-regulated (P < 0.01); The levels of cyclin B1, D1 protein were changed and cell cycle arrest was increased which mainly blocked in S phase. The interaction among the two drugs and DNA was in two different ways, leading to the curves of thermal denaturation of DNA changed significantly. Furthermore, the combination of Tan IIA and Api showed a stronger inhibitory effect on tumor volume and weight in S180 mice model than monotherapy, which was similar to cyclophosphamide therapy but less side effects. Conclusion Tan IIA combined with Api exerted synergistic antitumor effects. The two drugs interacted with DNA in different ways and aggravated the cell cycle arrest, which were the key mechanisms of their synergistic antitumor effects.

8.
Article in Chinese | WPRIM | ID: wpr-867051

ABSTRACT

Objective:To investigate the effect of adenosine deaminase acting on RNA 1 (ADAR1) on 5-serotonin-2c receptor in alleviating aggression in socially isolated mice.Methods:Sixty healthy male BALB / c mice aged 21 days were randomly divided into six groups: social isolation group, social control group, ADAR1 inducer social isolation group, ADAR1 inhibitor social isolation group, ADAR1 inducer social control group and ADAR1 inhibitor control group.The mice fed in single cage for 4 weeks were used as social isolation model while the mice fed in group were used as control group.ADAR1 inducer (5.0×10 4 U/kg) and inhibitor (10 mg/kg) were given intraperitoneally to mice in the ADAR1 inducer social isolation group and the ADAR1 inhibitor social isolation group respectively.The aggressive behavior of mice was evaluated by resident-intruder test.The expression of ADAR1 and 5-serotonin-2c receptors in the brain of mice was detected by immunohistochemistry and Western blot. Results:The attack latency of social isolation group was significantly lower than that of social control group ((43.15±6.99) s, (542.40±30.50) s; t=15.906, P<0.01), and the latency of attack ((256.70±29.49) s) in the ADAR1 inducer social isolation group was significantly higher than that in the social isolation group ( t=7.046, P<0.01). The latency of attack ((15.25±2.18)s) in the ADAR1 inhibitor social isolation group was significantly lower than that in the social isolation group ( t=3.809, P<0.01). The optical density of ADAR1 immunoreactive cells in the amygdala of the social isolation group mice was significantly lower than that in the corresponding brain area of the social control group (BLA: (0.038±0.002), (0.074±0.004); LaDL: (0.033±0.002), (0.060±0.002); LaVM: (0.045±0.003), (0.073±0.004); Lavl area: (0.044±0.003), (0.070±0.003); t=8.428, 9.037, 6.462, 5.698, all P<0.01). The optical density of ADAR1 immunoreactive positive cells in the amygdala (BLA: (0.060±0.003), LaDL: (0.042±0.002), LaVM: (0.056±0.004), Lavl: (0.054±0.003) in the ADAR1 inducer social isolation group was significantly higher than those in the corresponding brain area of the social isolation group mice ( t=6.055, 2.876, 2.312, 2.492; all P<0.05). The expression of ADAR1 protein and 5-serotonin-2c receptor protein in amygdala of social isolation group were significantly lower than those of social isolation group ( t=11.37, 12.65; P<0.01). The expression of ADAR1 protein and 5-serotonin-2c receptor protein in the amygdala of the ADAR1 inducer social isolation group were significantly higher than those of the social isolation group ( t=3.02, 4.401; P<0.05). Conclusion:ADAR1 inducer alleviates the aggressive behavior of social isolated BALB / c mice by enhancing the protein expression of 5-serotonin-2c receptor in the amygdala of social isolated BALB/c mice.

9.
Article in Chinese | WPRIM | ID: wpr-782451

ABSTRACT

OBJECTIVE@#To study the efficacy and safety of vitamin D as an adjuvant therapy for childhood pneumonia through a systematic review.@*METHODS@#Cochrane Library, PubMed, EMbase, CNKI, Wanfang Data, and Weipu Data were searched for randomized controlled trials (RCTs) of vitamin D as the adjuvant therapy for childhood pneumonia published up to August 2019. Literature screening, quality assessment, and data extraction were performed based on inclusion and exclusion criteria. Revman 5.3 was used to perform the Meta analysis of outcome indicators.@*RESULTS@#A total of 7 RCTs with 1 527 children were included, with 762 children in the vitamin D adjuvant therapy group and 765 children in the control group. The results of the Meta analysis showed that vitamin D adjuvant therapy had no effect on recovery time (P=0.67), length of hospital stay (P=0.73), and time to relief of fever (P=0.43). Furthermore, it did not reduce the recurrence rate (P=0.14), rate of adverse events (P=0.20), and mortality rate (P=0.98) of childhood pneumonia.@*CONCLUSIONS@#Current evidence shows that vitamin D adjuvant therapy has no marked efficacy in the treatment of childhood pneumonia.

10.
China Pharmacy ; (12): 2410-2414, 2020.
Article in Chinese | WPRIM | ID: wpr-825900

ABSTRACT

OBJECTIVE:To provide referenc e for developing pharmaceutical care of chronic kidney disease (CKD)by clinical pharmacists. METHODS :During 1st,Jan. to 30th,Sept. in 2019,inpatients diagnosed as CKD admitted or transferred to nephrology department of our hospital were selected. Medication reconciliation was conducted by clinical pharmacists after pharmaceutical consultation ,reviewing medical records ,analyzing and summarizing drug-related problem (DRP). Medication reconciliation recommendations were proposed to physician. Subsequently ,drug list and medical education were provided to patients by clinical pharmacists. RESULTS & CONCLUSIONS :The medication information of 130 patients was collected ,and 85 of them were provided with medication reconciliation by clinical pharmacist ,with a reconciliation rate of 65.38%. There were 193 medical orders involved 85 patients. Among all the DRP ,the top three of proportion in medical orders were improper drug usage and dosage (41.96%),drug interactions (18.13%)and improper drug selection (14.51%). Among drugs related to medication reconciliation,top three types were cardiovascular system drugs (26.94%),drug correcting mineral and bone metabolism disorder (22.28%)and antibacterial drugs (16.02%). The main plans of medication reconciliation were drug change (71 cases,36.78%), drug withdrawal (42 cases,21.76%),drug supplement (35 cases,18.13%),followed by change of medication time and dosage adjustment. The majority (88.08%)of the recommendations were accepted by physician and patients. Clinical pharmacists can reduce the occurrence of DRP by medication reconciliation. The majority of patients with CKD were elderly and complicated with multiple diseases and took various drugs ,and part of drugs required dose adjustment due to the change of kidney function ,therefor clinical pharmacists implementing medication reconciliation to CKD ,should pay attention to drug usage and dosage ,especially disorder,and assist physicians to ensure safety of drug use in

11.
Chinese Medical Journal ; (24): 285-293, 2019.
Article in English | WPRIM | ID: wpr-774852

ABSTRACT

BACKGROUND@#Pulmonary vein (PV) occlusion generally depends on repetitive contrast agent injection when cryoballoon ablation for atrial fibrillation (AF). The present study was to compare the effect of cryoballoon ablation for AF guided by transesophageal echocardiography (TEE) vs. contrast agent injection.@*METHODS@#Eighty patients with paroxysmal AF (PAF) were enrolled in the study. About 40 patients underwent cryoballoon ablation without TEE (non-TEE group) and the other 40 underwent cryoballoon ablation with TEE for PV occlusion (TEE group). In the TEE group during the procedure, PVs were displayed in 3-dimensional images to guide the balloon to achieve PV occlusion. The patients were followed up at regularly scheduled visits every 2 months.@*RESULTS@#No differences were identified between the groups in regard to the procedure time and cryoablation time for each PV. The fluoroscopy time (6.7 ± 4.2 min vs. 17.9 ± 5.9 min, P < 0.05) and the amount of contrast agent (3.0 ± 5.1 mL vs.18.1 ± 3.4 mL, P < 0.05) in the TEE group were both less than the non-TEE group. At a mean of 13.0 ± 3.3 mon follow-up, success rates were similar between the TEE group and non-TEE group (77.5% vs. 80.0%, P = 0.88).@*CONCLUSIONS@#Cryoballoon ablation with TEE for occlusion of the PV is both safe and effective. Less fluoroscopy time and a lower contrast agent load can be achieved with the help of TEE for PV occlusion during procedure.


Subject(s)
Aged , Atrial Fibrillation , Diagnostic Imaging , General Surgery , Contrast Media , Cryosurgery , Methods , Echocardiography, Three-Dimensional , Methods , Echocardiography, Transesophageal , Methods , Female , Humans , Male , Middle Aged , Pulmonary Veins , Diagnostic Imaging , General Surgery , Treatment Outcome
12.
Article in Chinese | WPRIM | ID: wpr-753180

ABSTRACT

Objective To analysis the distribution of posterior corneal astigmatism (PA) and evaluate the influence of keratometric astigmatism (KA) and total corneal astigmatism (TCA) on the calculation of Toric intraocular lens (Toric IOL) in patients with age-related cataract (ARC) and high corneal astigmatism.Methods An observational study design was adopted.Pentacam was used to measure 200 eyes of 181 patients with ARC and KA>0.75 D in Affiliated Hospital of Nantong University from August 2016 to April 2017.KA,PA,TCA and anterior corneal astigmatism (AA) were recorded.The astigmatism magnitude and axis of PA was studied.The subjects were divided into astigmatism with the rule group,astigmatism against the rule group and oblique astigmatism group according to the axis of AA.The correlations of decomposition values between PA and AA or KA and TCA in each group were analyzed by Pearson linear correlation analysis.The difference of decomposition values between KA and TCA in each group was compared by paired sample t test.The type and axis of Toric IOL were calculated by online formula according to KA and TCA.This study followed the Declaration of Helsinki and written informed consent was obtained from each patient prior to any medical examination.Results The mean astigmatic magnitudes of PA was -0.32 D×93.1°.PA exceeded 0.5 D in 22 eyes (11%).The steepest posterior corneal meridian was vertically aligned in 163 eyes (81.5%).The decomposition value KP(0) and KP (45) of PA were positively correlated with those ofAA (r=0.480,P<0.001;r=0.251,P<0.001).The mean astigmatic magnitudes of KA and TCA were 1.44D×89.6° and 1.32 D×89.5° in astigmatism with the rule group,1.39 D×153.4° and 1.71 D×154.4° in astigmatism against the rule group and 1.13 D× 122.0° and 1.24 D× 124.2° in oblique astigmatism group.53 eyes (69.7%) had KA higher than TCA in astigmatism with the rule group.82 eyes (87.3%) had KA lower than TCA in astigmatism against the rule group;20 eyes (66.7%) had KA lower than TCA in oblique astigmatism group.There were significant differences in KP (0) between KA and TCA in different astigmatism groups (all at P<0.001).The calculated Toric IOL type were inconsistent in 85 eyes(42.5%) and the calculated axis were inconsistent in 176 eye s (88.2%).Conclusions In patients with high corneal astigmatism,the astigmatism type of PA is mostly astigmatism against the rule.Ignoring the PA can lead to deviation of Toric IOL type selection and axis placement in some patients.For patients who cannot measure PA or TCA,the type of Toric IOL should be adjusted appropriately.

13.
Article in Chinese | WPRIM | ID: wpr-745732

ABSTRACT

Endocrine-disrupting chemicals ( EDCs ) are widely found in people's daily supplies or environments. The individuals in the world contact with these substances through different pathways. Adolescent-developed individuals are more sensitive to these substances. The integration of many environmental factors through epigenetic modification affects the starting age of puberty. Although there are various related studies, it is impossible for humans to contact only a single substance, and there is still much doubt about the additive effect of EDCs mixtures with similar mechanisms of action. Therefore, analytical scientists, molecular biologists, toxicologists, epidemiologists, endocrinologists, gynecologists, obstetricians, and oncologists are required to conduct multidisciplinary research to address and to discuss all the scientific results. It may have far-reaching implications for formulating the prevention strategies and individualized health plans.

14.
Chinese Journal of Geriatrics ; (12): 322-326, 2019.
Article in Chinese | WPRIM | ID: wpr-745516

ABSTRACT

Objective To investigate the effects of transplantation with different amount of bone marrow mesenchymal stem cells(BMSCs)on osteoporosis in ovariectomized rats.Methods The rat model of osteoporosis was prepared by ovariectom(OVX)and verified after 3 months.A total of 60 female Sprague-Dawley rats were randomly divided into 6 groups(n=10 each).The rats with shamed operation served as a sham-injured control group(sham control group).The 5 ovariectom (OVX) groups with osteoporosis were study groups as follows:(1)the negative therapy group(simple OVX group);(2)the positive therapy group(OVX+ estrogen,E2 group) by intramuscular injection;others were treated with transplantation of BMSCs by tail vein injection in low dose(LS group),middle dose (MS group)and high dose(HS group).At 8,12 and 16 weeks after intervention,body mineral density (BMD)were detected by dual-energy x-ray absorptiometry scans.After 16 weeks of intervention,rat shinbone was obtained and stained by hematoxylin-eosin(HE) staining.Results Compared with the sham control group,simple OVX group showed a reduced total body BMD and the decreased proportion of trabecular bone to bone marrow cavity area (P <0.05).The total body BMD and the proportion of trabecular bone to bone marrow cavity area were higher in each BMSCs transplantation groups than in simple OVX group at 8,12,16 weeks after intervention(P <0.05),which showed a increased trend in the total body BMD and the proportion with the increased dose of transplantation BMSCs(P<0.05).Rats in the HS group had highest BMD and best proportion of trabecular bone to bone marrow cavity area among three doses of transplantation BMSCs.Conclusions BMSCs transplantation can significantly improve osteoporosis of ovariectomized rats with an increased total body BMD and higher proportion of trabecular bone to bone marrow cavity area,and better and longer outcomes can be achieved.

15.
Acta Pharmaceutica Sinica ; (12): 159-165, 2019.
Article in Chinese | WPRIM | ID: wpr-778660

ABSTRACT

The objective of this paper was to establish a level A in vitro-in vivo correlation (IVIVC) for goserelin acetate extended release microspheres for injection. Three kinds of goserelin acetate microspheres with different release rates were prepared and the critical physicochemical properties, such as drug loading, particle size, glass transition temperature and morphology were characterized. In vitro dissolution test of the prepared goserelin acetate microspheres was performed using sample-and-separate method at 45 ℃ in 5% (v/v) methanol. The morphology of the microspheres and the molecular weight of poly (lactic-co-glycolic acid) (PLGA) of the prepared goserelin acetate microspheres were investigated to research the release mechanism of microspheres. The plasma concentration of goserelin was detected after intramuscular injection of goserelin acetate microspheres to SD rats, and correlated with the in vitro release profiles after processing by percent AUC method. The pharmacokinetic experimental protocol of goserelin acetate microspheres for injection in SD rats was approved by the Animal Ethics Committee of Shandong Luye Pharmaceutical Co., Ltd. The results indicated that the developed sample and separate method was able to detect differences in the release characteristics of the prepared goserelin acetate microspheres, and the in vitro-in vivo correlation of goserelin acetate microspheres was excellent (r > 0.98) and had good predictive ability in SD rats.

16.
Acta Pharmaceutica Sinica ; (12): 151-158, 2019.
Article in Chinese | WPRIM | ID: wpr-778657

ABSTRACT

This study was designed to prepare a novel lipid bilayer coated hollow mesoporous silica nanocarrier for co-delivery of gene drugs and chemotherapeutic drugs to enhance the inhibitory activity of antitumor drugs in hepatoma cells. Hollow mesoporous silica was synthesized by modified StÖber method. Lipid-fusion principle was used to prepare lipid-hollow co-loaded doxorubicin (DOX) and miR-375 (LHMSN-DOX/miR-375). Meanwhile, the morphology, particle size, surface potential, drug loading and release were characterized in vitro. The inhibition of cell proliferation, cell migration and invasion was then evaluated. The results indicated that the core-shell structure of LHMSN-DOX/miR-375 was clear with an intact outer lipid membrane and an ordered internal HMSN mesoporous structure. The drug release amount was pH responsive while the drug was rapidly released under simulated intracellular acidic conditions relative to normal physiological environment. Compared with free DOX, LHMSN-DOX/miR-375 can deliver DOX and miR-375 to liver cancer cells and inhibit the proliferation, migration and invasion of cells more effectively.

17.
Tianjin Medical Journal ; (12): 1201-1205, 2019.
Article in Chinese | WPRIM | ID: wpr-815595

ABSTRACT

@#Objective To investigate the effect of artesunate inhibiting the expression of FOXP3 on proliferation, apoptosis and multidrug resistance of adriamycin (ADR) -resistant K562/ADR cells in chronic myeloid leukemia (CML), and to explore its mechanism. Methods The expressions of FOXP3 mRNA in K562 and K562/ADR cells were detected by real-time PCR. The expressions of FOXP3 proteins in K562 and K562/ADR cells were detected by Western blot assay. The K562/ADR cells were treated with different concentrations of artesunate (2.5, 5.0, 7.5, 10.0 and 12.5 mg/L) for 24 h. The toxicities of different concentrations of artesunate to K562/ADR cells were detected by CCK-8 assay, and the non-cytotoxic concentrations were screened. The expressions of FOXP3 mRNA and proteins in K562/ADR cells treated by non-cytotoxic concentration of artesunate were detected by RT-PCR and Western blot assay. The changes of toxicities of ADR in K562/ ADR cells were detected by CCK-8 assay. The average fluorescence intensities of ADR were detected by FCM assay. Results The expressions of FOXP3 were higher in K562/ADR cells than those in K562 cells. The mRNA and proteins expressions of FOXP3 were significantly lower in 2.5 mg/L group, 5 mg/L group and 7.5 mg/L group than those in the control group. The toxicities and concentrations of ADR were increased in K562/ADR cells treated by artesunate (both P<0.05). Conclusion FOXP3 gene is highly expressed in adriamycin-resistant K562/ADR cells in CML. Artesunate can increase the concentrations of ADR and reverse multidrug resistance in K562/ADR cells by inhibiting the expression of FOXP3 in a dose-dependent manner.

18.
Article in Chinese | WPRIM | ID: wpr-700331

ABSTRACT

Objective To evaluate the differences of the malignant rate of thyroid nodules between primary hyperparathyroidism (PHPT) patients and health examination population, and analyze the clinical characteristics of PHPT combined with papillary thyroid carcinoma (PTC). Methods The clinical data of 158 PHPT patients who had underwent parathyroidectomy and 468 thyroid nodule patients who had underwent fine-needle aspiration (FNA) from 2009 to 2014 were retrospectively analyzed, and the detection rate of PTC in patients with thyroid nodule was recorded. Results Of the 158 patients with PHPT, 61 patients had thyroid nodules, the incidence of PHPT with thyroid nodule was 38.6% (61/158), and postoperative pathologically proved PTC was in 12 cases (19.7%). Of the 468 thyroid nodule patients who had underwent FNA, postoperative pathologically proved PTC was in 19 patients (4.1% ). The malignant rate of thyroid nodules in PHPT patients was significantly higher than that in health examination population (P<0.01). The adjusted serum calcium in patients with PHPT combined with PCT was significantly lower than that in patients with PHPT combined with benign thyroid nodules and patients with PHPT without thyroid nodules: (2.63 ± 0.26) mmol/L vs. (2.92 ± 0.32) and (2.93 ± 0.47) mmol/L, and there was statistical difference (P<0.05). Conclusions The malignant rate of thyroid nodules in PHPT patients is significantly higher than that in patients from health examination population, which suggests that PHPT might be a risk factor for the malignancy of thyroid nodules. A lower level of serum calcium may predict the existence of PCT in PHPT patients with thyroid nodules.

19.
Article in Chinese | WPRIM | ID: wpr-699579

ABSTRACT

Corneal astigmatism includes anterior astigmatism (AA) and posterior astigmatism (PA).According to the hterature,85.0%-96.1% PA ranging from-0.01 to-1.10 D refers to astigmatism against the rule.With the age increasing,PA shows a significant trend from astigmatism against the rule to with the rule,while AA shows toward against-the-rule astigmatism.Therefore,PA often leads to partially decrease of total astigmatism (TA) in young adults,but the increase of TA in aged elderly.Clinically,ignorance of PA will lead to a false estimate of TA,thereby affecting visual quality after refractive cataract surgery.And this article aims to introduce the measurement of PA and its clinical significance for providing the reference in clinical practices.

20.
Article in Chinese | WPRIM | ID: wpr-704149

ABSTRACT

Objective To explore the effects of ADAR1 inducer and inhibitor on cognition and ADAR1 expression of isolated BALB/c mice.Methods Sixty healthy BALB/c mice were divided into 6 groups according to randomized design with 10 animals each group,the gregarious control group (GH),social isolation model group (SI),ADAR1 inducer treated gregarious group (GH+IFN-γ),ADAR1 inhibitor treated gregarious group (GH+EHNA),ADAR1 inducer treated isolation group (SI+IFN-γ) and ADAR1 inhibitor treated isolation group (SI+EHNA).Mice in drug treatment groups were treated with ADAR1 inducer (5.0? 104 U/kg,20 ml/kg,ip) and inhibitor (10 mg/kg,20 ml/kg,ip).Objection recognition test was used to measure cognition.Immunohistochenmistry was used to measure ADARI immunoreactivity and Western blotwas used to measure ADAR1 protein expression.Results In the objection recognition test,the non-spatial discrimination index of mice in SI group (-0.16±0.09) was significantly lower than that of GH group (0.41 ±0.17,P<0.01),the non-spatial discrimination index of mice in SI+IFN-γ group (0.20±0.09) and in SI+ EHNA group (-0.29±0.12) was higher (P<0.01) and lower (P<0.05) than that of the SI group respectively.The immunohistochemistry results showed that the ADAR1 immunoreactivity in hippocampus of mice in SI group (Hilus:(0.013±0.003),CAI:(0.021±0.005)) decreased significantly compared to those of GH group(Hilus:(0.021 ±0.002),(0.047±0.004);both P<0.05).And GH+IFN-γgroup mice showed increased ADAR1 immunoreactivity obviously in Hilus ((0.013±0.003) vs (0.023±0.004),P<0.01) and in CA1 ((0.021±0.005) vs (0.040±0.005),P<0.01) compared with that of SI group,ADAR1 inducer recovered the above abnornal ADAR1 immunoreactivity.Western blot results showed that the ADAR1 protein expression of mice in SI group (0.48 ±0.07) in hippocampus was significantly decreased (P<0.01) compared to that of GH group (1.00 ±0.00).The level of ADAR1 protein in SI+IFN-γgroup(0.82 ±0.04) increased compared with that of SI group.Conclusions Four weeks of social isolation can reduce the non-spatial cognitive ability of BALB/c mice and decrease the expression of ADAR1 in the hippocampus.The ADAR1 inducers and inhibitors can reverse and aggravate the cognitive impairment caused by social isolation respectively.The related mechanisms may be related to the expression of ADAR1.

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