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1.
Chinese Journal of School Health ; (12): 190-193, 2022.
Article in Chinese | WPRIM | ID: wpr-920586

ABSTRACT

Objective@#To analyze the duration and influencing factors of moderate and vigorous physical activity(MVPA) on weekends for primary school students in grades 4 to 6 in Beijing, and to provide a reference for formulating health education and promotion measures.@*Methods@#Multi stage stratified cluster sampling method was used to randomly select 2 515 students from grades 4-6 in 14 primary schools in Beijing, and a self administered questionnaire was used to record MVPA on weekend, social demographic characteristics, other related health behaviors and knowledge. Multivariate Logistic regression analysis was used to explore the influencing factors of MVPA on weekends.@*Results@#The prevalence of insufficient MVPA on weekends in Beijing was 63.54 %, and the prevalence was higher among girls (69.92%) than boys (57.81%) ( χ 2=39.65, P <0.01). Multiple Logistic regression analysis revealed that girls ( OR =1.74), living in rural areas ( OR =1.41), participants attending general schools ( OR = 1.34 ), from divorced family ( OR =1.46), and short sleep duration ( OR =1.50) were more likely to fail to meet the MVPA recommendations( P <0.05).@*Conclusion@#It is quite common that no sufficient weekend MVPA among senior primary school students, among them, the outer suburbs and schools with relatively weak teaching resources are the key places that need attention, and girls are the key groups that need attention.

2.
Chinese Journal of School Health ; (12): 129-132, 2022.
Article in Chinese | WPRIM | ID: wpr-920519

ABSTRACT

Objective@#To obtain the prevalence of hyperuricemia among primary and secondary school students in Shandong Province, and to provide data support for the prevention and treatment of hyperuricemia in children and adolescents.@*Methods@#The stratified cluster random sampling method was used to collect the data of 3 609 primary and secondary school students in urban and rural areas in Shandong Province, including the blood uric acid, waist circumference, height, and weight.@*Results@#The average blood uric acid value of primary and secondary school students was (316.17±82.57)μmol/L, and the total detection rate of hyperuricemia was 17.4%. The detection rate of blood uric acid was 18.9% and hyperuricemia[(338.26±90.30)μmol/L] of boys were higher than those of girls[(294.25±67.29)μmol/L,15.9%], and the difference were statistically significant( t/χ 2=16.60, 5.48, P < 0.05). The detection rate of blood uric acid (21.6%) and hyperuricemia [(353.24±78.98)μmol/L] in urban areas was higher than that in rural areas, and higher in coastal areas[(376.80±85.46)μmol/L, 26.6%] than inland; the differences were statistically significant ( t =14.54, 15.27, χ 2=48.15, 132.53, P <0.01). The differences in the blood uric acid value and the detection rate of hyperuricemia between different ages were statistically significant ( t/χ 2=11.79, 18.11, P <0.01). The detection rate of blood uric acid increased with the increase of obesity, waist circumference,blood pressure,blood lipids and blood sugar,and the difference were statistically significant ( χ 2=999.95, 561.08 , 447.57, 196.37, 115.08, P <0.01).@*Conclusion@#The detection rate of hyperuricemia among primary and secondary school students in Shandong Province is relatively high. The hyperuricemia is related to gender, age, BMI, waist circumference, blood pressure,blood lipids and blood sugar. Highrisk groups should have regular physical examinations to actively improve their unhealthy lifestyles and reduce the incidence of hyperuricemia.

3.
International Eye Science ; (12): 505-508, 2022.
Article in Chinese | WPRIM | ID: wpr-920442

ABSTRACT

@#AIM: To observe the difference of curative effect between internal limiting membrane(ILM)inversion and insertion and ILM peeling and to analyze the influence of different surgical methods on the morphology of hiatus healing.<p>METHODS: A retrospective analysis of the clinical data of 22 patients with macular hole, who received vitrectomy in the First People's Hospital of Zunyi City from June 2017 to June 2020. According to the surgical methods, they were divided into 8 cases and 8 eyes in the ILM peeling group and 14 cases and 14 eyes in the ILM inversion covering group. The two groups of patients underwent best corrected visual acuity(BCVA)test, non-contact intraocular pressure and optical coherence tomography(OCT)examination before and 1wk, 1, and 3mo after operation were analysed. <p>RESULTS: The macular hole closure rate in the ILM peeling group was 75%, and the hole closure rate in the ILM inversion and insertion group was 93%. There was no statistically significant difference in the hole closure rate between the two groups(P=0.527). The trends of BCVA over time in the two groups were similar, but there were differences in time(Ftime=18.426, Ptime<0.001)and no difference between groups and interactions(Fbetween groups=1.319, Pbetween groups=0.289; Fbetween groups×time=1.658, Pbetween groups×time=0.211). The BCVA of the two groups was statistically significant 1wk after operation compared with that before operation(t= -2.200, -3.092; all P<0.05); The BCVA of the ILM inversion and insertion group was improved 3mo after operation compared with that before operation, and the difference in the group was statistically significant(t=2.503, P=0.019). There was no difference in overall foveal thickness between the two groups after surgery(Ftime=1.054, Ptime=0.346; Fbetween groups=0.110, Pbetween groups=0.750; Fbetween groups×time=2.391, Pbetween groups×time=0.152).<p>CONCLUSION: Both ILM inversion and insertion and ILM peeling can effectively increase the rate of macular hole closure. The macular structure healing after the ILM inversion and insertion is better, and the postoperative visual function improvement is more satisfactory.

4.
China Pharmacy ; (12): 344-351, 2022.
Article in Chinese | WPRIM | ID: wpr-913094

ABSTRACT

OBJECTIVE To evaluate the efficacy and safety of dydrogesterone in the treatment of dysmenorrhea. METHODS The prospective ,random-controlled,open-labeland multicenter clinical study was adopted. A total of 108 women with dysmenorrhea were randomly assigned into dydrogesterone group and control group according to the ratio of 1∶1,with 54 patients in each group. Dydrogesterone group was treated with dydrogesterone 10 mg orally ,twice a day ,on the 5th-25th day of menstrual cycle ,for 3 menstrual cycles. Control group received Guizhi fuling capsule 0.93 g orally ,three times a day,since the end of menstrual bleeding to the third day of the next menstruation ,for 3 menstrual cycles. Main results were the changes of visual analogue scale (VAS)scores in 2 groups after 3 menstrual cycles ;secondary results were the changes of COX menstrual symptom scale (CMSS),quality life of 36-item short form (SF-36),levels of carbohydrate antigen 125(CA125)and interleukin 6(IL-6)after 3 menstrual cycles ;other findings included additional benefits and drug safety. RESULTS The results of intention to analysis data set and the follow-up study protocol analysis data set showed that VAS scores of 2 groups after treatment of dysmenorrhea for 1,2 and 3 menstrual cycles were lower than those before treatment ,the longer the treatment time ,the more obvious the decrease of VAS score (P<0.05),and VAS score decline of dydrogesterone group was better than that of control group(P<0.05). After 3 menstrual cycles ,both the two group showed significant reduction in the severity and duration scores of CMSS(P<0.05);and the decrease of the above scores in the dydrogesterone group was superior than in the control group (P< 0.05). After 3 menstrual cycles ,among 8 dimensions of SF- 36 scale,the scores of 7 dimensions in dydrogesterone group were significantly higher than those before treatment ,such as the scores of physiological function ,physical role ,physical pain , emotional function ,social function ,general health status and energy (P<0.05);the increase of the scores of four dimensions were higher than those in the control group ,such as physical pain ,social function ,general health status ,energy(P<0.05). There was no significant difference in the levels of CA 125 and IL- 6 between 2 groups before and after treatment (P>0.05). After 3 menstrual cycles,the menstrual cycle and menstrual period in the dydrogesterone group were shorter than those before treatment ,and the menstrual volume decreased (P<0.05);but there was no significant change in the above indexes of control group (P>0.05). After 3 menstrual cycles ,the incidence of adverse drug events and adverse reactions in dydrogesterone group was 32.69%(17/52)and 28.85%(15/52);no serious adverse drug events or adverse reactions such as thrombosis occurred in both groups. CONCLUSIONS Dydrogesterone can effectively reduce the VAS score ,also relieve dysmenorrhea-related symptoms ,and improve the quality of life. The efficacy of dydrogesterone is superior than that of Guizhi fuling capsule in treatment for dysmenorrheal ,without serious adverse reactions. It is well tolerated.

5.
Article in Chinese | WPRIM | ID: wpr-943091

ABSTRACT

ObjectiveTo evaluate the metabolic stability of lucidin by incubating liver microsomes and liver S9 from 4 species, and to compare the species differences in metabolism of lucidin in vitro. MethodA qualitative and quantitative method of lucidin based on ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) was established and verified. Lucidin was incubated with rat, mouse, beagle dog, human liver microsomes and liver S9 to investigate the metabolic stability parameters, metabolites, metabolic pathways. ResultHepatic clearance (CLh) of lucidin was in order of mouse>rat>beagle dog>human in both phase Ⅰ and phase Ⅱ incubation system. Its metabolic stability was good in rat, beagle dog and human, while it showed metabolic instability and moderate metabolic stability in mouse microsomes and liver S9, respectively. A total of 5 metabolites were rapidly identified, including 3 oxidation metabolites of phase Ⅰ and 2 sulfation metabolites of phase Ⅱ. The production rate of metabolites was consistent with the results of metabolic stability. ConclusionThe established UHPLC-HRMS is simple and specific, which can be used for the study on the metabolic stability and metabolites of lucidin. Its metabolic stability and metabolite production rate in vitro are significantly different among species, the metabolic characteristics of rat and beagle dog are similar to human, which provides an important reference for subsequent research in vivo, safety evaluation and animal model selection of lucidin.

6.
China Pharmacy ; (12): 2230-2234, 2022.
Article in Chinese | WPRIM | ID: wpr-943063

ABSTRACT

OBJECTIVE To establish quantitative analysis of multi -components by single marker (QAMS) method to simultaneously detect the contents of cinnamic acid ,cinnamaldehyde,plantamajoside,verbascoside,isoacteoside,calceolarioside B , psoralen,isopsoralen,neobavaisoflavone and bavachin in Gushen dingchuan pill ,and to perform quality evaluation of Gushen dingchuan pill by combining with chemical pattern recognition . METHODS High-performance liquid chromatography was adopted . Using psoralen as internal standard ,the relative correction factors of the other 9 components were established ,and the contents of each component were calculated and compared with those determined by external standard method . Cluster analysis ,principal component analysis and partial least squares discrimination analysis were performed by the results of QAMS method ,and the qualities of 15 batches of Gushen dingchuan pills were evaluated . RESULTS The above 10 components showed a good linear relationship in their respective ranges (r>0.999 0). RSDs of precision ,repeatability,stability and recovery tests were all lower than 2.00%. There was no significant difference between QAMS method and external standard method (P>0.05). The results of cluster analysis and principal component analysi showed that 15 batches of Gushen dingchuan pills could be clustered into 3 categories. The results of partial least squares discrimination analysis showed that psoralen ,verbascoside,cinnamaldehyde and isopsoralen were the main potential markers affecting the quality of Gushen dingchuan pills . CONCLUSIONS Established QAMS method for quantitative control of multi index components and chemical pattern recognition can be used for the quality evaluation of Gushen dingchuan pills .

7.
Article in Chinese | WPRIM | ID: wpr-942366

ABSTRACT

Objective To establish an animal model of sparganosis mansoni through oral administration of Cyclops infected with procercoids. Methods Domestic cats were infected with Sparganum mansoni under laboratory conditions, and fresh cat stool samples were collected, washed in dechlorinated water, and filtered. Spirometra mansoni eggs were collected and prepared into suspensions. Twenty C57BL/6j mice were randomly divided into the experimental group (n = 15) and the control group (n = 5). Wild Cyclops were infected with Spirometra mansoni coracidia to allow 3 to 5 procercoids in each Cyclop. Then, each mouse in the experimental group was given 15 Cyclops infected with procercoids by gavage, while mice in the control group were orally administered with the same volume of dechlorinated water. All mice were sacrificed after 5 months, and dissected, and suspicious Sparganum mansoni worms were collected. The serum specific IgG antibody against Sparganum mansoni was measured in mice using enzyme-linked immunosorbent assay (ELISA). Genomic DNA was isolated from suspicious Sparganum mansoni worms, and the specific Sparganum mansoni cytochrome oxidase I (COI) gene was amplified using PCR assay. Results Among the 15 mice in the experimental group, six were positive for the serum specific IgG antibody against Sparganum mansoni, and milky white worms were found and collected from the subcutaneous regions of 4 out of 6 mice. Only one worm was detected in each mouse, and the worm morphology was similar to Sparganum mansoni. Capillary electrophoresis of the PCR amplification products of COI gene presented a specific band with 151 bp in size, and sequencing analysis revealed 100% homology with Sparganum mansoni. Conclusions A mouse model of sparganosis mansoni is successfully created through oral administration of Cyclops infected with Spirometra mansoni procercoids.

8.
Article in Chinese | WPRIM | ID: wpr-942350

ABSTRACT

Stroke is a common cerebrovascular disease, characterized by high incidence, mortality and disability rate. Neuronal cells, the basic unit of the central nervous system, can be injured to varying degrees when stroke occurs. Neuronal cell injury after stroke is also the key cause leading to neurological dysfunction, affecting the prognosis and quality of life of patients. Therefore, reducing the neuronal cell injury and delaying the process of cell death are effective to decrease the nerve function injury in stroke patients and improve their prognosis, thus lowering the death and disability rate of stroke. Ferroptosis is a new form of cell death that has been widely concerned in recent years. Several studies have confirmed that there is ferroptosis in neuronal cells after stroke. Since ferroptosis is an adjustable form, its intervention can help regulate the injury and death of neuronal cells. Studies have shown that inhibiting ferroptosis plays a role in protecting neuronal cells. Traditional Chinese medicine (TCM), with the multi-channel and multi-target treatment advantages, has been widely used in the whole stroke and has achieved good clinical efficacy. It might be a new direction taking TCM regulation of ferroptosis as the entry point for stroke treatment in the future. This review revealed the mechanism of ferroptosis, discussed the research status of TCM in intervening in neuronal cell ferroptosis, and provided reference for further improving the efficacy of TCM in stroke.

9.
Journal of Clinical Hepatology ; (12): 1843-1846, 2022.
Article in Chinese | WPRIM | ID: wpr-941547

ABSTRACT

Objective To summarize the clinical features of patients with Wilson's disease (WD). Methods A retrospective analysis was performed for the clinical data of 83 patients with WD who were admitted to The Third Hospital of Hebei Medical University from April 2013 to August 2021, including clinical manifestations, Imaging examinations, laboratory examinations, liver histopathological examinations, and ATP7B gene testing results. The patients were divided into groups based on different clinical types. A one-way analysis of variance was used for comparison between groups. Results The youngest age was 3 years for the 83 patients with WD, among whom 39 (46.99%) had an age of ≤18 years, with a mean age of 21.16±14.87 years for all 83 patients. Of all patients, 63.86% had liver-type WD, 31 patients (37.35%) had developed liver cirrhosis at the time of consultation, and 5 patients (6.2%) attended the hospital due to acute or acute-on-chronic liver failure. Of all patients, 62(74.69%) were positive for corneal K-F ring, and the positive rate of K-F ring was 66.04% in the patients with liver-type WD. Among the 83 patients, 79(95.18%) had a reduction in blood ceruloplasmin, and 73(87.95%) had an increase in 24-hour urine copper. The liver histopathological results of 25 patients showed varying degrees of inflammation, fibrosis, steatosis, and copper particle deposition in liver tissue. The ATP7B gene testing results of 25 patients showed that c.2333G > T/p.R778L of exon 8 was the most common mutation site. Conclusion Most patients with WD have the manifestation of liver diseases, and the examinations of corneal K-F ring, serum ceruloplasmin, and 24-h urine copper have their own limitations. Liver pathology and ATP7B gene testing can be performed when it is unable to make a confirmed diagnosis.

10.
Acta Pharmaceutica Sinica ; (12): 2751-2758, 2022.
Article in Chinese | WPRIM | ID: wpr-941502

ABSTRACT

This study investigated the inhibitory effect and mechanisms of cryptotanshinone (CPT) on tamoxifen resistant cell MCF7-TAMR. The inhibitory effect of CPT on the viability of MCF7-TAMR cells was evaluated using the MTT assay. We found that CPT significantly inhibited the growth of MCF7-TAMR cells in a dose- and time-dependent manner. The half inhibitory concentration (IC50) is 15.14 ± 2.82 μmol·L-1 at 24 h. CPT induced cell cycle arrest of MCF7-TAMR cells at G0/G1 phase, and promoted apoptosis of MCF7-TAMR cells by upregulating intracellular levels of reactive oxygen species (ROS). Transwell results showed that CPT significantly inhibited the migration of MCF7-TAMR cells. Furthermore, CPT decreased the CD24-/lowCD44+ cell population in MCF7-TAMR cell-derived microspheres. Western blot results showed that CPT effectively inhibited the phosphorylation of estrogen receptor α (ER-α), and reduced the expression of phosphatidylinositol 3-kinase (PI3K-p85), serine-threonine protein kinase (Akt) and multidrug transporter ATP-binding cassette superfamily G member 2 (ABCG2). These results showed that CPT can induce cell apoptosis, cause cell cycle arrest, inhibit cell migration and inhibit ER-α phosphorylation, inhibit PI3K/Akt signaling pathway, reduce the number of CD24-/lowCD44+ cells and the expression of ABCG2, overcome cell drug resistance.

11.
Article in Chinese | WPRIM | ID: wpr-940752

ABSTRACT

Citri Reticulatae Pericarpium is a commonly used traditional Chinese medicine in China. By referring to herbal monographs and related research documents, and comparing all editions of Chinese Pharmacopoeia and local processing specifications, the processing history, modern processing specifications, study on chemical constituents and pharmacological effects before and after processing of Citri Reticulatae Pericarpium were summarized. It was found that the processing methods of Citri Reticulatae Pericarpium in the past dynasties were mainly cleansing, cutting and frying. The processing methods of Citri Reticulatae Pericarpium included in all editions of Chinese Pharmacopoeia were all cleansing and cutting. In modern local processing specifications, there were mainly processing methods such as frying, steaming and carbonizing. The components of Citri Reticulatae Pericarpium were mainly volatile oil and flavonoids. After processing, the total amount of volatile oil generally decreased, and the contents and compositions of volatile oil and flavonoids also changed. It may be the main reason for the difference of efficacy before and after processing. At present, the optimization of processing technology of Citri Reticulatae Pericarpium mainly focuses on steaming, processing with vinegar and baking. Pharmacological research on Citri Reticulatae Pericarpium mainly focuses on reducing blood lipid, relieving cough, relieving asthma and resolving phlegm, and inhibiting pulmonary fibrosis. The current research on the processing methods of Citri Reticulatae Pericarpium is not in-depth enough, the material basis of many processing methods has not been clarified, and the research on the efficacy before and after processing is not in-depth enough. Further research is needed to clarify the material basis and mechanism after the processing of Citri Reticulatae Pericarpium, so as to standardize the processing method and establish specific quality standards.

12.
Article in Chinese | WPRIM | ID: wpr-940645

ABSTRACT

Diabetic nephropathy (DN) is among the common microvascular complications of diabetes. In recent years, the incidence has been on the rise with the increase in prevalence of diabetes, threatening the health of human. The early stage of DN is characterized by excessive accumulation of extracellular matrix (ECM) and thickening of glomerular basement membrane which result in glomerular mesangial proliferation and massive collagen deposition. The late stage features glomerular sclerosis and renal fibrosis (RF). It has been confirmed that RF is the key pathological process for the development of DN. Therefore, it is the research focus to explore the pathogenesis and treatment methods of RF. It has been frequently verified that Chinese medicine is superior in the treatment of diabetic RF. It relieves diabetic RF by regulating transforming growth factor-β (TGF-β), secretory glycoprotein (Wnt)/β-catenin, nuclear factor-κB (NF-κB), Notch, mitogen-activated protein kinase (MAPK), and other signaling pathways. Therefore, this paper reviews the pathogenesis of diabetic RF and the treatment with Chinese medicine, which is expected to serve as a reference for clinical application of Chinese medicine in the treatment of diabetic RF.

13.
Article in Chinese | WPRIM | ID: wpr-940622

ABSTRACT

ObjectiveTo explore the effect of Gegen Qinliantang (GQL) on vulnerable plaque of atherosclerosis based on the macrophage pyroptosis mediated by nuclear factor (NF)-κB/NOD-like receptor protein 3 (NLRP3)/cysteine-aspartic acid protease (Caspase)-1 pathway. MethodA total of 12 normal C57BL/6CNC mice were used as the control group, and 60 ApoE-/- mice of the same line were randomized into 5 groups: model group, low-dose, medium-dose, and high-dose GQL groups (GQL-D, GQL-Z, GQL-G groups, respectively), and western medicine group. The control group and model group were given (ig) equal volume sterile distilled, and GQL-D, GQL-Z, GQL-G and western medicine groups received (ig) corresponding concentration of drugs for 8 weeks. Aortic plaques were observed based on hematoxylin and eosin (HE) staining. Serum levels of interleukin (IL)-1β and IL-18 were detected by enzyme-linked immunosorbent assay (ELISA), protein levels of macrophage mannose receptor (CD206)/apoptosis-associated speck-like protein containing a CARD (ASC) and CD206/NLRP3 by double-labeling immunofluorescence, and C-terminal gasdermin D (GSDMD), N-terminal GSDMD, NLRP3, pro-cysteinyl aspartate specific proteinase 1 (pro-Caspase-1) and NF-κB p65 by Western blot. ResultCompared with the control group, model group demonstrated serious pathological changes, rise of the levels of serum IL-1β and IL-18 and tissue ASC, NLRP3, C-terminal GSDMD, N-terminal GSDMD, pro-Caspase-1, and NF-κB p65, and decrease of CD206 level (P<0.05). As compared with model group, the administration groups showed alleviation of the lesions in aortic wall, decrease in levels of serum IL-1β and IL-18 and tissue ASC, NLRP3, C-terminal GSDMD, N-terminal GSDMD, pro-Caspase-1, and NF-κB p65, and rise of CD206 level, with significant difference between some groups (P<0.05). ConclusionGegen Qinliantang alleviates vulnerable plaque of atherosclerosis by regulating NF-κB/NLRP3/Caspase-1 pathway and further relieving macrophage pyroptosis.

14.
Article in Chinese | WPRIM | ID: wpr-940569

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ObjectiveTo investigate the potential pharmacological mechanism of Xinmaikang tablets in the treatment of atherosclerosis cardiovascular disease by using network pharmacology and cell experimental validation. MethodThe components of Xinmaikang tablets were searched by BATMAN-TCM database and the active ingredients and potential targets were screened. The atherosclerosis related disease targets were searched in GeneCards and online mendelian inheritance in man(OMIM) disease databases. The therapeutic targets were obtained by mapping the intersection of the tablets and disease targets. Therapeutic targets were uploaded to STRING database to construct protein-protein interaction(PPI) network. Cytoscape software was used to create a "drug-active component-therapeutic target" network map, and a network topology algorithm was used to screen key action targets. David software was used for gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) function enrichment analysis. The key targets of drug therapy were validated by in vitro cell assay. ResultA total of 19 active ingredients, 132 potential targets and 4703 atherosclerotic disease-related target genes of Xinmaikang tablets were retrieved and screened, and 84 intersection targets were obtained. 3 key therapeutic targets of Xinmaikang tablets in the treatment of atherosclerotic diseases were screened, including Calmodulin 1(CALM1), voltage-dependent L-type calcium channel subunit alpha-1C(CACNA1C) and Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform(PIK3CA). A total of 313 biological processes, 89 molecular functions and 53 cell components were obtained by GO enrichment. A total of 40 pathways were obtained from KEGG functional enrichment, including purine metabolism, renin secretion, CGMP/PKG signaling pathway and so on. In vitro cell experiment results verified that Xinmaikang tablets can up-regulate the expression of CALM1 and CACNA1C, down-regulate the expression of PIK3CA, so as to inhibit the activity of inflammatory response, and play a therapeutic role in atherosclerotic diseases. ConclusionXinmaikang tablets may treat atherosclerosis cardiovascular disease through betulin, methyleugenol and other compounds, through purine metabolism, renin secretion, cGMP/PKG signaling pathway and other pathways, which acts on CALM1, CACNA1C, PIK3CA and other targets.

15.
Article in Chinese | WPRIM | ID: wpr-940463

ABSTRACT

ObjectiveTo predict the pharmacodynamic basis and core target of Shengxiantang in the treatment of myasthenia gravis (MG) by network pharmacology and molecular docking and to further verify the molecular mechanism through animal experiment. MethodThe active components and potential targets of Shengxiantang were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), and the disease-related targets from GeneCards and other databases. Then the common targets of the decoction and the disease were screened out, followed by the construction of protein-protein interaction (PPI) network, and Gene Ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of the common targets based on STRING database and Cytoscape 3.8.2. Afterward, Cytoscape 3.8.2 was employed to construct the disease-active component-target network. AutoDock and PyMOL were used for molecular docking of key components and hub genes. Finally, we used the Rα97-116 peptide to induce experimental autoimmune myasthenia gravis (EAMG) in rats and then verified the core target yielded in the docking with the model rats. ResultA total of 655 disease-related targets, 118 active components of the decoction, 21 common targets of the disease and the decoction, and 3 hub genes were screened out. The common targets were mainly involved in the GO terms of regulation of active oxygen metabolism, positive regulation of protein transport, and positive regulation of protein localization, and the KEGG pathways of toll-like receptor signaling pathway, phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway, hypoxia inducible factor-1 (HIF-1) signaling pathway, and T cell receptor signaling pathway. The results of molecular docking showed that quercetin and Akt1 had the lowest and stable binding energy and interacted with each other through the amino acid residue LYS-30. Western blot demonstrated that Shengxiantang significantly inhibited the expression of p-Akt protein in the spleen of EAMG rats. ConclusionThe pharmacological mechanism of Shengxiantang in the treatment of MG may be that the main chemical components regulate the expression of the core protein Akt, and then may participate in and affect PI3K/Akt signaling pathways, laying a theoretical and experimental basis for further research.

16.
Article in Chinese | WPRIM | ID: wpr-940455

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ObjectiveTo investigate the inhibitory effect of Astragalus polysaccharide (APS) on epithelial-mesenchymal transition (EMT) induced by transforming growth factor-β1 (TGF-β1) in cisplatin (DDP)-resistant lung adenocarcinoma cell line A549/DDP cells transplanted into nude mice and the molecular mechanism in improving DDP resistance. MethodBALB/c nude mice were randomly divided into a blank group, a model group, a DDP group, and a combination group (APS combined with DDP). A549/DDP cells were infected with TGF-β1-overexpressed lentiviral vector and the negative control. The infected cells were inoculated subcutaneously in nude mice. The A549/DDP cells with TGF-β1 gene overexpression were inoculated into all groups except the control group with negative TGF-β1 gene overexpression. The drug intervention was performed eight days after cell inoculation. The mice in the combination group received intragastric administration of APS (0.3 g·kg-1·d-1) and intraperitoneal injection of cisplatin (0.003 5 g·kg-1), and those in the cisplatin group received intraperitoneal injection of cisplatin (0.003 5 g·kg-1). After 32 days of cell inoculation, the nude mice were killed and the tumor tissues and lungs were collected. The tumor weight was recorded and the inhibition rate was calculated. The number of metastatic nodules of the lung tumor on the whole slide was counted under the microscope. Immunohistochemistry, Western blot, and real-time fluorescence-based quantitative polymerase chain reaction (Real-time PCR) were used to detect the protein and gene expression of EMT molecular markers α-catenin and N-cadherin, and tumor drug resistance markers human lung resistance protein (LRP), multidrug resistance-associated protein (MRP), and P-glycoprotein (P-gp) in the transplanted tumor. ResultCompared with the blank group, the model group showed increased tumor weight and metastatic nodules of the lung tumor (P<0.05), decreased protein and mRNA expression of α-catenin (P<0.05), and elevated protein and mRNA expression of N-cadherin, LRP, MRP, and P-gp (P<0.05). Compared with the model group and the cisplatin group, the combination group showed reduced tumor weight and metastatic nodules of the lung tumor (P<0.05), increased protein and mRNA expression of α-catenin (P<0.05), and decreased protein and mRNA expression of N-cadherin, LRP, MRP, and P-gp (P<0.05). ConclusionAPS can inhibit the growth and metastasis of the transplanted tumor of lung adenocarcinoma and improve cisplatin resistance, which may be related to the inhibition of EMT of tumor cells.

17.
Article in Chinese | WPRIM | ID: wpr-940353

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ObjectiveTo explore the effects of the main component of Realgar arsenic disulfide (As2S2) on DNA methylation of SKM-1 cells with myelodysplastic syndrome. MethodCell Counting Kit-8 (CCK-8) was used to detect the inhibitory effect of As2S2(0, 1, 2, 4, 8, 16 μmol·L-1)on SKM-1 cells. Propidium iodide (PI) staining was applied to detect the effect of As2S2(0, 1, 2, 4 μmol·L-1)on the SKM-1 cell cycle. The effect of As2S2 (0, 4 μmol·L-1) on the methylation of SKM-1 cells on a genome-wide scale was observed by using Human Methylation 850K BeadChip, followed by Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) analyses. According to the microarray data, the antioncogene TUSC3 was selected, and real-time quantitative polymerase chain reaction (Real-time PCR) and Western blot were adopted to investigate the effect of As2S2 (0, 1, 2, 4 μmol·L-1) on the mRNA and protein expression of TUSC3, respectively. ResultCompared with the conditions in the blank group, As2S2 inhibited SKM-1 cells, increased the proportion of cells in the G0/G1 phase, and decreased the proportion of cells in the S phase(P<0.05). The 850K microarray showed that 4 μmol·L-1 As2S2 could significantly induce DNA methylation in SKM-1 cells, with 12 710 differentially methylated genes involved (50% hypermethylated and 50% hypomethylated genes). KEGG and GO analyses showed that differentially methylated genes were involved in many important biological functions and signaling pathways, including purine metabolism, natural killer cell-mediated cytotoxicity, endocytosis, chemokine signaling pathway, and nuclear ubiquitin ligase complex. In terms of downstream gene expression, Real-time PCR and Western blot showed that As2S2 increased the expression of TUSC3, as compared with the conditions in the blank group (P<0.05). ConclusionAs2S2, the main component of Realgar, has a significant regulatory effect on the methylation of SKM-1 cells, which is presumedly achieved by increasing the expression of TUSC3.

18.
Article in Chinese | WPRIM | ID: wpr-940295

ABSTRACT

ObjectiveTo explore the regulatory effect of Quyu Huatan Tongmai prescription on intestinal mircoflora of hyperlipidemia golden hamster and scientific evidence for the compatibility. MethodSyrian golden hamsters were randomized into normal, model, prescription, stasis-dispelling (Quyu), phlegm-dissolving (Huatan), and detoxification (Jiedu) groups, with 8 in each group. Hyperlipidemia in golden hamsters was induced by high-fat diet (4 weeks). Then hamsters in the Quyu group (1.11 g·kg-1), Huatan group (0.39 g·kg-1), Jiedu group (0.07 g·kg-1), and prescription group (1.42 g·kg-1) were given (ig) corresponding drugs and those in the normal group and the model group received (ig) distilled water of equivalent volume, once a day for 6 weeks. Serum lipids were determined, and hematoxylin-eosin (HE) staining was used to observe the pathological morphology of the liver. Feces were collected for 16S rRNA gene high-throughput sequencing of intestinal flora. ResultCompared with normal group, the model group demonstrated increase in body weight (P<0.05, P<0.01) and blood lipids (P<0.01), decrease in intestinal flora diversity (P<0.05, P<0.01), and variation of the relative abundance of intestinal flora at phylum, family, and genus levels (P<0.05, P<0.01). Compared with the model group, Quyu Huatan Tongmai prescription controlled the body weight change, reduced the serum triglyceride (TG), total cholesterol (TC), and low density lipoprotein cholesterol/high density lipoprotein cholesterol ratio (LDL-C/HDL-C) (P<0.05, P<0.01), improved the structure of intestinal flora, decreased the ratio of Firmicutes to Bacteroides (P<0.01), raised the abundance of Bacteroidaceae, Porphyromonadaceae, Rikenellaceae, and Pasteurella (P<0.05, P<0.01), and lowered the relative abundance of Coriobacterium (P<0.05) in hyperlipidemia golden hamsters. All the split prescriptions improved blood lipids and intestinal flora of the hamsters and particularly, the lipids-lowering effect of the Jiedu group and the regulation of flora in the Huatan group were closer to those of the prescription group. ConclusionQuyu Huatan Tongmai prescription and the split prescriptions all alleviated the hyperlipidemia of golden hamsters to different degrees possibly by regulating intestinal flora structure and improving intestinal microecology. The effect of the prescription group was most significant, and coming in second was the Huatan group. This study also provides scientific evidence for the effect of Quyu Huatan Tongmai prescription.

19.
Article in Chinese | WPRIM | ID: wpr-939993

ABSTRACT

ObjectiveTo investigate the changes of bone mineral density of distal femur and proximal tibia in patients with spinal cord injury. MethodsNine inpatients with spinal cord injury in Beijing Bo'ai Hospital for rehabilitation from November, 2018 to January, 2021 were recruited. The bone mineral density of distal femur, proximal tibia, total hip and femoral neck at admission and six months after admission was measured. ResultsCompared with the results of admission, the bone mineral density of distal femur, proximal tibia, total hip and femoral neck decreased significantly six months after admission (∣Z∣ > 2.265, P < 0.01). The percentage of decreased bone mineral density in the femoral neck was inversely correlated with the lower extremity movement score at the second measurement (r = -0.515, P = 0.035). ConclusionWithin one year after the onset of spinal cord injury, the bone mineral density of distal femur and proximal tibia decreases.

20.
Article in Chinese | WPRIM | ID: wpr-939641

ABSTRACT

OBJECTIVES@#To evaluate the clinical effect of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in children with hyper-IgM syndrome (HIGM).@*METHODS@#A retrospective analysis was performed on the medical data of 17 children with HIGM who received allo-HSCT. The Kaplan Meier method was used for the survival analysis of the children with HIGM after allo-HSCT.@*RESULTS@#After allo-HSCT, 16 children were diagnosed with sepsis; 14 tested positive for virus within 100 days after allo-HSCT, among whom 11 were positive for Epstein-Barr virus, 7 were positive for cytomegalovirus, and 2 were positive for JC virus; 9 children were found to have invasive fungal disease. There were 6 children with acute graft-versus-host disease and 3 children with chronic graft-versus-host disease. The median follow-up time was about 2 years, and 3 children died in the early stage after allo-HSCT. The children had an overall survival (OS) rate of 82.35%, an event-free survival (EFS) rate of 70.59%, and a disease-free survival (DFS) rate of 76.47%. The univariate analysis showed that the children receiving HLA-matched allo-HSCT had a significantly higher EFS rate than those receiving HLA-mismatched allo-HSCT (P=0.019) and that the children receiving HLA-matched unrelated allo-HSCT had significantly higher OS, EFS, and DFS rates than those receiving HLA-mismatched unrelated allo-HSCT (P<0.05). Compared with the children with fungal infection after allo-HSCT, the children without fungal infection had significantly higher EFS rate (P=0.02) and DFS rate (P=0.04).@*CONCLUSIONS@#Allo-HSCT is an effective treatment method for children with HIGM. HLA-matched allo-HSCT and active prevention and treatment of fungal infection and opportunistic infection may help to improve the prognosis of such children.


Subject(s)
Child , Epstein-Barr Virus Infections , Graft vs Host Disease/prevention & control , Hematopoietic Stem Cell Transplantation/methods , Herpesvirus 4, Human , Humans , Hyper-IgM Immunodeficiency Syndrome , Retrospective Studies
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