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1.
Article in Chinese | WPRIM | ID: wpr-921738

ABSTRACT

The terpenoids in Pogostemon cablin have complex structures and abundant pharmacological effects. Patchouli alcohol(PA) and pogostone(PO) have a high medicinal value by virtue of anti-tumor, anti-inflammatory, antibacterial, antioxidant, and other biological activities. Due to the low content of terpenoid metabolites in P. cablin, the study of biosynthesis and metabolism regulation can provide a biosynthetic basis for obtaining high-content terpenoids. In this study, key enzyme genes in biosynthesis, transcription factors in metabolism regulation, spatio-temporal expression of terpene synthase were reviewed, aiming to provide a reference for the development, protection, and utilization of P. cablin resources.


Subject(s)
Pogostemon/genetics , Terpenes , Transcription Factors/genetics
2.
Article in Chinese | WPRIM | ID: wpr-921559

ABSTRACT

Objective To establish an artificial intelligence model based on B-mode thyroid ultrasound images to predict central compartment lymph node metastasis(CLNM)in patients with papillary thyroid carcinoma(PTC). Methods We retrieved the clinical manifestations and ultrasound images of the tumors in 309 patients with surgical histologically confirmed PTC and treated in the First Medical Center of PLA General Hospital from January to December in 2018.The datasets were split into the training set and the test set.We established a deep learning-based computer-aided model for the diagnosis of CLNM in patients with PTC and then evaluated the diagnosis performance of this model with the test set. Result The accuracy,sensitivity,specificity,and area under receiver operating characteristic curve of our model for predicting CLNM were 80%,76%,83%,and 0.794,respectively. Conclusion Deep learning-based radiomics can be applied in predicting CLNM in patients with PTC and provide a basis for therapeutic regimen selection in clinical practice.


Subject(s)
Artificial Intelligence , Humans , Lymph Nodes/diagnostic imaging , Lymphatic Metastasis , Retrospective Studies , Risk Factors , Thyroid Cancer, Papillary/diagnostic imaging , Thyroid Neoplasms/diagnostic imaging
3.
Article in Chinese | WPRIM | ID: wpr-793061

ABSTRACT

To explore the value of trans-rectal shear wave elastic mode combined with elastic modulus in the diagnosis of prostate cancer and establish a new method for the evaluation of prostate with trans-rectal shear wave elastography(SWE). The typical findings of trans-rectal ultrasound(US)and SWE in 79 patients with prostate cancer(=41)and benign prostatic hyperplasia(BPH)(=38)confirmed by surgery or US-guided biopsy were analyzed retrospectively.Their diagnostic value were evaluated with the pathological results as the golden standards. Three or more malignant features detected by conventional trans-rectal US(=42.5,<0.001)and asymmetrical SWE mode(=54.2,<0.001)showed statistically significant difference in prostate cancer and BPH groups.The elastic modulus of Emean and Emax in the prostate cancer group were(92.8±21.5)and(114.2±29.8)kPa,which were significantly higher than those in the BPH group [(56.7±14.0)(=-8.8,<0.001)and(68.4±17.2)kPa(=-8.3,<0.001)].The receiver-operating characteristic(ROC)curve with Logistic regression showed that the elastic model combined elastic modulus had the largest area under ROC curve and the highest diagnosis efficiency of prostate cancer,with the cutoff value of 0.45.The diagnosis sensitivity,specificity,positive predictive value,negative predictive value,and accuracy of the combination were 95.1%,89.5%,90.7%,94.4%,and 92.4%,respectively. Combination of SWE mode and elastic modulus is more valuable than elastic modulus alone in the diagnosis of prostate cancer.

4.
Article in Chinese | WPRIM | ID: wpr-878840

ABSTRACT

Trace metals deficiency or excess are associated with the etiology and pathogenesis of rheumatoid arthritis(RA). Aconiti Radix Cocta(A) and Paeoniae Radix Alba(B) are commonly used together for the treatment of RA. In this study, we aim to determine anti-arthritic-related metal bioavailability in the compatibility of herb A and B for avoiding metal deficiency or excess, and optimize the combination ratio of herb A and B, accordingly. Anti-arthritic-related metal bioaccessibility were evaluated by in vitro simulator of all gastrointestinal tract(including mouth, stomach, small and large intestines), and the roles of gastrointestinal digestive enzymes and intestinal microflora were investigated. Anti-arthritic-related metal bioavailability was assessed by the affinity adsorption with liposomes. The results indicated that compatibility proportion of corresponding herbal plants, gastrointestinal digestion and microbial metabolic, which could affect metal digestion and absorption. The optimal compatibility proportion of 1 A∶1 B is recommended, according to the dose of anti-arthritic-related metal bioavailability, which is often chosen for clinical practice of RA therapy. Thus, anti-arthritic-related metal bioavailability might be the key active substances for RA treatment.


Subject(s)
Aconitum , Biological Availability , Drugs, Chinese Herbal , Paeonia
5.
Journal of Medical Postgraduates ; (12): 397-400, 2019.
Article in Chinese | WPRIM | ID: wpr-818249

ABSTRACT

Objective The aim of this article was to investigate the effect of nasal endoscopic surgery (NES) using low-temperature plasma radiofrequency (LTPRF) in the treatment of juvenile nasopharyngeal angiofibroma (JNA). Methods We retrospectively analyzed the clinical data on 38 cases of JNA treated by NES+LTPRF (n = 26) or conventional surgical resection (control, n = 12) in Drum Tower Hospital of Nanjing University School of Medicine from January 2012 to December 2018. We compared the operative time, postoperative Lund-Mackay endoscopy scores (LMES), incidence of complications, postoperative recovery, rate of recurrence, and Visual Analogue Scale (VAS) scores between the two groups of patients. Results The rates of LMES gradesⅠ and Ⅱ were significantly higher while those of grades Ⅲ and Ⅳ remarkably lower in the NES+LTPRF than in the control group (P < 0.05). Compared with the controls, the patients of the NES+LTPRF group showed markedly shorter time of nasal mucosa epithelialization ([3.5 ± 0.1] vs [2.3 ± 0.1] mo, P < 0.05) and lower rates of postoperative complications (38.5% vs 7.7%, P < 0.05), recurrence (16.7% vs 3.8%, P < 0.05), and VAS scores at 3 days, 2 months, 6 months and 12 months after surgery (P < 0.05). Conclusion NES+LTPRF is an effective strategy for the treatment of juvenile nasopharyngeal angiofibroma and worthy of a wide clinical application.

6.
Article in Chinese | WPRIM | ID: wpr-843377

ABSTRACT

Objective:To investigate the protective effect of gastrin on rat chondrocytes treated with interleukin-1β (IL-1β) and underlying mechanism. Methods:Chondrocytes of knee joint of newborn rats were isolated and cultured. The effect of gastrin on cell viability was determined by MTT assay. In addition, after being induced with 10 ng/mL of IL-1β for 2 d, the chondrocytes were treated with 1×10-7 mol/L of gastrin for 3 d. The expressions of chondrocyte catabolic genes, i.e., matrix metalloproteinase 3 (MMP3) and MMP13, and the key proteins in gastrin-related signaling pathway, i.e., cholecystokinin B receptor (CCKBR), extracellular regulated protein kinases (ERK), phospho-ERK (p-ERK) and p65 were detected by real-time PCR and Western blotting, respectively. Results:Gastrin with the concentration of 1×10-7 mol/L had no cytotoxic effects on the viability of chondrocytes. Based on IL-1β induction, the expressions of MMP3 and MMP13 genes were significantly downregulated by gastrin. Meanwhile, the expression of CCKBR in the IL-1β induction group and gastrin treatment groups was higher than that in the normal control group. Gastrin also facilitated the phosphorylation of ERK and resulted in the inhibition of P65. Conclusion:Gastrin increases the phosphorylation level of ERK by activating its receptor CCKBR, thereby inhibiting the activity of P65 in NF-κB pathway, antagonizing the chondrocyte catabolic activity induced by IL-1β. Accordingly, gastrin may possess the potentials of cartilage matrix protection via inhibiting mRNA expressions of MMP3 and MMP13 and catabolism in rat chondrocytes.

7.
Article in Chinese | WPRIM | ID: wpr-695655

ABSTRACT

Objective·To investigate therapeutic effect of gastrin on steroid-associated osteonecrosis (SAON) in rat model. Methods·Twenty-four SD rats were randomly divided into three groups i.e. normal control group (normal group), SAON control group (SAON group) and SAON treatment group (treatment group). SAON group and treatment group were intravenously injected with lipopolysaccharide 1 time per day (600 μg/kg) for 2 d and meanwhile intramuscularly injected with methylprednisolone 1 time per day (50 mg/kg) for 3 d. Normal group was injected with normal saline of the same volumns. After steroid injections, treatment group was injected with gastrin 1 time per day (800 μg/kg) for 14 d, while SAON group was injected with normal saline of the same volumns. After the treatment, bone trabeculas below femoral head growth plate were dissected in the rats for bone histology. Hematoxylin-eosin (H-E) staining, immunohistochemistry, fluorescence staining and Goldner's trichrome staining were applied in this study. Results·SAON model in rats was successfully established. The result of H-E staining showed that compared with SAON group, thrombus area, number and area of fat cells in the bone marrows of treatment group obviously decreased (all P<0.05). Immunohistochemistry showed that osteogenic transcription factor (Sp7) positive cells in treatment group were more than those in SAON group (P<0.01). Compared with SAON group, osteoid length and area (Goldner′s trichrome staining), and bone formation rate and bone mineralization deposition rate (fluorescence staining) all significantly increased in treatment group (all P<0.01). Conclusion·Gastrin can effectively treat SAON in rats by suppressing thrombus and lipid formation and enhancing bone-formation.

8.
Article in Chinese | WPRIM | ID: wpr-300411

ABSTRACT

<p><b>OBJECTIVE</b>To study the reference ranges of six sex hormones, i.e., luteinizing hormone, follicle-stimulating hormone, progesterone, prolactin, estradiol, and testosterone, for healthy children aged 0-18 years in Shenzhen, China.</p><p><b>METHODS</b>Stratified cluster sampling was performed to select 2 178 healthy children aged 0-18 years in the districts of Futian, Luohu, Nanshan, Bao'an, and Longgang in Shenzhen between September 2015 and September 2016. There were 1 219 boys and 959 girls, including 81 neonates, 335 infants, 346 young children, 469 preschool children, 419 school-aged children, and 528 adolescents. The American Beckman DXI800 chemiluminescence meter was used to measure the levels of luteinizing hormone, follicle-stimulating hormone, progesterone, prolactin, estradiol, and testosterone.</p><p><b>RESULTS</b>There were significant differences in the levels of luteinizing hormone, follicle-stimulating hormone, progesterone, prolactin, estradiol, and testosterone between different age groups (P<0.05). There were also significant differences in the levels of these sex hormones between boys and girls in the same age group (P<0.05). The reference ranges of six sex hormones were established for healthy children aged 0-18 years in Shenzhen based on the levels of these hormones in different age groups.</p><p><b>CONCLUSIONS</b>There are significant differences in sex hormones between different age groups or sex groups. The reference ranges of six sex hormones established for different sexes or ages have great significance in the diagnosis and treatment of endocrine diseases in children.</p>


Subject(s)
Adolescent , Age Factors , Child , Child, Preschool , Estradiol , Blood , Female , Follicle Stimulating Hormone , Blood , Gonadal Steroid Hormones , Blood , Humans , Infant , Infant, Newborn , Luminescent Measurements , Luteinizing Hormone , Blood , Male , Progesterone , Blood , Reference Values , Testosterone , Blood
9.
Article in Chinese | WPRIM | ID: wpr-273785

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the mechanism by which propofol exposure causes PC12 cell apoptosis under hypoxic conditions.</p><p><b>METHODS</b>PC12 cells were exposed to room air, 35% oxygen, or 5% oxygen (hypoxia) for 24 h in the presence of either 10 µmol/L lipid emulsion or 10 µmol/L propofol. After the treatments, the cell apoptosis was measured by flow ceytometry, and the level of reactive oxygen species (ROS) and the activity of superoxide dismutase (SOD) were evaluated.</p><p><b>RESULTS</b>In room air, PC12 cells treated with propofol showed increased apoptosis rate and ROS production as compared with the cells treated with the lipid emulsion; propofol treatment of the cells exposed to 35% oxygen showed obvious enhancement of the apoptosis rate, ROS production and SOD activity. Under the hypoxic condition, propofol treatment even further increased the apoptosis rate, ROS production and SOD activity. Lipid emulsion caused no such changes in cells exposed to room air, 35% oxygen or 5% oxygen.</p><p><b>CONCLUSION</b>Under hypoxic conditions, propofol can cause apoptosis in PC12 cells by inducing oxidative stress injury.</p>


Subject(s)
Animals , Apoptosis , Cell Hypoxia , Oxidative Stress , PC12 Cells , Propofol , Pharmacology , Rats , Reactive Oxygen Species , Metabolism , Superoxide Dismutase , Metabolism
10.
Chinese Pharmaceutical Journal ; (24): 1350-1354, 2014.
Article in Chinese | WPRIM | ID: wpr-859964

ABSTRACT

OBJECTIVE: To establish a method for determining methanol, ethanol and methylene dichloride residues of low molecular weight heparin(LMWH) by gas chromatography.

11.
Article in English | WPRIM | ID: wpr-296020

ABSTRACT

<p><b>OBJECTIVE</b>To study environment-friendly determination of azobenzene in trace amounts using beta-cyclodextrin (beta-CD)-modified Au electrode.</p><p><b>METHODS</b>beta-CD-modified Au electrode was fabricated with a two-step approach, and then a gold electrode modified with beta-CD was used to detect azobenzene by employing Osteryoung square wave voltammetry.</p><p><b>RESULTS</b>The modified electrode could detect azobenzene, showing a good linearity between the electrochemical current and concentration.</p><p><b>CONCLUSION</b>Although the electrochemical current is related with concentration, the detection limit is around 1.0 x 10(-10) mol/L. This study may provide a new environment-friendly approach for monitoring water quality.</p>


Subject(s)
Azo Compounds , Chemistry , Electrochemistry , Electrodes , Environmental Monitoring , Methods , Gold , Chemistry , Reproducibility of Results , Sensitivity and Specificity , Water Supply , Reference Standards , beta-Cyclodextrins , Chemistry
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