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Article in Chinese | WPRIM | ID: wpr-807972


Objective@#To study the genome molecular characteristics of Getah virus (SC1210) which isolated in Sichuan province in 2012.@*Methods@#Reverse transcriptase polymerase chain reaction (RT-PCR) was used to identify the isolate and the genome was sequenced by the second Ion Torrent PGM. Computer softwares, including Mega Align and Mega 6, were used to analyze the nucleotide and deduced amino acid sequence, and draw phylogenetic trees.@*Results@#SC1210 was identified as Getah virus. The full genome sequence was 11 690nt, the nucleotide and amino acid homology of the full sequence with other strains were 99.2%-99.7% and 96.5%-99.4%.The capsid protein of SC1210 consisting of 804 nucleotides, encoding 268 amino acids and the full-length of E2 protein, had 1 266 nucleotides, encoding 422 amino acids. The nucleotide homology of the capsid protein and the E2 protein with other strains were 94.9%-99.2% and 94.6%-99.6%, and the amino acid were 97%-99.6% and 97.1%-99.5%. The 3′ UTR of the virus included 402 nucleotides and there were three repeat sequence elements and 19 nucleotides conservation sequence.@*Conclusions@#The first GETV isolate SC1210 in Sichuan province has a closer relationship with Yunnan strain YN040 and a far genetic relationship with MM2021.

China Oncology ; (12)1998.
Article in Chinese | WPRIM | ID: wpr-536594


Purpose:To explore the problems in the treatment of the elderly patients suffering from colonic carcinoma.Methods:182 cases of elderly patients aged over 70 who received operation for colonic carcinoma during Jan 1994 to Dec 1998 were analyzed retrospectively. Results:176 cases underwent resections (resection rate 96.7%), of which 129 cases(70.9%)had radical resection and 47 cases(25.8%)palliative resection. 6 cases(3.3%)experienced simple colostomy or bypass. Postoperative complications were found in 67 cases (36.8%),of which 8 cases died during perioperation period(mortality rate 4.4%). Conclusions:Old age is not the major determining factor when considering surgical operation as the treatment for colonic carcinoma patients. In order to enhance resection rate especially radical resection rate and survival rate,to diminish postoperative complications and mortality, and thus to improve the quality of life for the elderly colonic carcinoma patients, a series of measures must be taken. First, diagnosis and treatment of the associated conditions must be emphasized. Second, full preoperative preparations with appropriate choice of the time and procedure for operation, as well as exact operation principles must be followed. Finally, intensive monitoring during the perioperative period and prevention and treatment of postoperative complications were also important factors to be considered.