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Journal of Chinese Physician ; (12): 1167-1169, 2015.
Article in Chinese | WPRIM | ID: wpr-482765


Objective To explore the effect of black garlic extract on HeLa cells and its possible molecular mechanisms.Methods Inhibitory rate of HeLa cells was detected with methyl thiazolyl tetrazolium(MTT).Flow cytometry annexin V-fluorescein isothiocyanate/propidium iodide (V-FITC/PI) was used to detect tumor cell apoptosis.Flow cytometry was used to measure cell-cycle changes.Immunohistochemistry method was used to detect expressions of tumor proteins bax and Bcl-2.Results Black garlic extract significantly inhibited the growth of HeLa cells.Black garlic extract significantly induced apoptosis of HeLa cells.The apoptosis rate was (53.26 ± 1.78)% in the black garlic high-dose group,and (3.68 ±0.11)% in the control group.Black garlic extract affected cell cycle,upregulated bax expression,and downregulated bcl-2 expression.Conclusions Black garlic extract significantly induced the apoptosis of HeLa cells.This effect might be caused through increasing G2/M cells or changing expressions of bax and bcl-2.

Article in Chinese | WPRIM | ID: wpr-407581


BACKGROUND: Foreign scholars use thermotherapy, a new pathway for synthetic therapy of tumor, to perform hyperpyrexia combined with chemical drug radiotherapy of intraperitoneal infiltration and metastasis or to study on the therapeutic effect of metaphase and advanced stage tumor. Especially, establishing thermal biology and thermal dosiology is a scientific and quantified track for synthetic therapy of thermal radiotherapy, thermal chemotherapy and hyperthermal perfusion of tumor.OBJECTIVE: To analyze the thermal dose effect of regional radiofrequency (RF) hyperthemia combined with radiotherapy, chemotherapy and thermal perfusion on metaphase and advanced stage tumor.DESIGN: Controlled observation.SETTING: Key Department of Traditional Chinese and Western Medicine for Tumor, the 107 Hospital of Chinese PLA (General Center for Non-traumatic Treatment and Diagnosis of Tumor).PARTICIPANTS: Totally 1 455 patients with metaphase and advanced-stage tumor admitted to the General Center for Non-traumatic Treatment and Diagnosis of Tumor, the 107 Hospital of Chinese PLA between June and September 2006 who received conservative palliative treatment in the Department of Internal Medicine were recruited in this study. They were all confirmed by pathology and imageology. ECOG was scored 2 to 4 points. Informed consents of detection and treatment were obtained from all the involved patients. The study was approved by the Hospital Ethics Committee.According to the therapeutic regimen, the patients were assigned into thermal perfusion group (n =53), thermal radiotherapy group (n =874), thermal chemotherapy group (n =458) and simple hyperthemia group (n =70).METHODS: After admission, patients in each group were performed peritoneal and pelvic cavity perfusion,intensity-modulated radiation therapy, routine chemotherapy and integrated traditional and western medicine palliative therapy, respectively. Meanwhile, they received RF hyperthemia using in vitro endogenic magnetic field hyperthermia system. A thermocouple was placed in the abdominal cavity. Temperature at 3 different sites in the abdominal cavity was collected, and meanwhile two different sites for measuring temperature at rectum and external acoustic meatus were monitored. The maximal temperature (Tmax), minimal temperature (Tmin) and the average temperature (Tave) of therapeutic target were recorded. 40 ℃/min was used as thermal dose, thermal perfusion was performed once a week, and RF hyperthemia was conducted twice a week, 60 minutes once. Target temperature was 39.5 to 43 ℃, and the thermal dose of 40 ℃/min was calculated out. Intensity-modulated radiation therapy was conducted within 1 hour before heating at (3-5)Gy/time, 3 to 4 times a week. Total dose was DT 30 to 50Gy. Thermal perfusion was conducted 2 to 3 times a week, and intensity-modulated radiation therapy was conducted once to twice a week and 6 to 8 times a course of treatment. The thermal effect of each patient was analyzed at different temperature and different diseases.MAIN OUTCOME MEASURES: ① To analyze the effect of effective thermal dose. ② To evaluate the therapeutic effect and the improvement of quality of life according to World Health Organization (WHO) objective therapeutic effect evaluation criteria and Zubrod-ECOG-WHO scores. ③To observe the changes in abdominal dropsy.RESULTS: Altogether 1 455 patients with metaphase and advanced stage participated in the final analysis. ①After perfusion, body temperature was over 39.5 ℃ in most cases after 15-minute warming, over 40.0 ℃ after another 25-minute warming, over 41.0 ℃ after additional 35-minute arming, and even to 43.0 ℃ after frequent such a thermal therapy. Temperature over 41 ℃ was found in 91 cases, at 40 ℃-40.9 ℃ in 414 cases and at 39.5 ℃-39.9 ℃ in 950 cases. ② The thermal effect of 40 ℃/min could obviously improve the therapeutic effects of graded intensity-modulated radiation therapy, low-dose chemotherapy and thermal perfusion group. The total effective rate of thermal perfusion group, thermal radiotherapy group, thermal chemotherapy group and simple hyperthemia group was 81.6%, 81.9%, 80% and 50%, respectively. The clinical therapeutic effect of 40 ℃-40.9 ℃ and 39.5 ℃-39.9 ℃ in the thermal perfusion group, thermal radiotherapy group and thermal chemotherapy group was significantly better than that in the simple hyperthemia group (P < 0.05). ③ Comparison of Zubrod-ECOG-WHO score of patients before and after therapy: After therapy, Zubrod-ECOG-WHO score was enhanced in 76.3% patients. ④The changes in abdominal dropsy of patients before and after therapy: After therapy, abdominal dropsy changed in 75% patients.⑤The therapeutic effects of different diseases at different temperature: For the same disease, the therapeutic effects over 41 ℃ and at 40 ℃-40.9 ℃ were significantly different from those at 39.5 ℃-39.9 ℃(P < 0.05); However, the therapeutic effects over 41 ℃ were not significantly different from those at 40 ℃-40.9 ℃ (P > 0.05).CONCLUSION: ①The effects of regional RF hyperthemia combined with intensity-modulated radiation therapy are confirmed in the treatment of metaphase and advanced-stage of tumor. ② The therapeutic effects strengthen correspondingly with 40 ℃/min thermal dose increase or temperature increase.

Article in Chinese | WPRIM | ID: wpr-595531


Objective To discuss the factors that affect the examination of urine formed elements by IQ200 automated uri-nalysis analyzer causing false positive and look for the solution methods. Methods 2,319 urine samples were tested by IQ200 automated urinalysis analyzer and microscopy. The results were contrasted and the false positive reasons were ana-lyzed. Results When using IQ200 automated urinalysis analyzer,the false positive rate of red cell is 14.7%. Crystals,bac-teria,yeast like fungus sometimes are regarded as red cell by analyzer. The false positive rate of white cell is 15.75%. Small round cells,lots of bacterium can cause false positive; the false positive rate of cast is 38.58 % and viscose rayon is main agent. Conclusion Many factors can affect the examination in IQ200 automated urinalysis analyzer and cause false positive,so IQ200 can only be used for scanning,which is very important to observe sediments by microscopic examination when pathologic ingredients in urine are increased and at the same time all above interferences are positive and manifold.

Article in Chinese | WPRIM | ID: wpr-587093


A special method of clinical tumor radiation therapy,namely 3DCRT& IMRT,is described in this paper,including its design and clinical application.

Article in Chinese | WPRIM | ID: wpr-412053


Current bioprosthetic heart valves implanted with viable human cells can be categorized into two basic designs, tissue engineering heart valve and porcine aortic valve implanted with viable human cells. Tissue engineering heart valve is constructed by seeding a synthetic polyglycolic acid fiber based biodegradable scaffold with viable human autologous cells. The fibroblasts are first seeded on valve scaffold. When the fibroblasts reached confluence, endothelial cells are seeded onto the surface of leaflets and grown into a single monolayer. Porcine aortic valve implanted with viable human cells is generated by removing native cells from tissue and then repopulating the tissue with viable human autologous cells. The method of removing native cells from fresh porcine aortic valve comprises subjecting the valve tissue to a hypotonic/hypertonic treatment, then treating the valve tissue with an enzyme-based solution. Isolated cell lines are cultured and thereafter such a valve matrix is implanted with fibroblasts, followed by endothelial cells. Bioprosthetic heart valves implanted with viable human cells do not produce and adverse immune response by the recipient upon implant, and possesses the regenerative capabilities.

Article in Chinese | WPRIM | ID: wpr-674104


Objective:To study on effects of Welling Granule on union of peptic ulcer and the gastrical ulcer induced by acetic acid in the rat. Methods:With random grouping method,the clinical recurrence rate of peptic ulcer,and regenerated macosa of healing gastric ulcer in the rat was investigated quantitatively with hematoxylin-eosin staining,and gastric wall combining macus amount,gastric acid and pepsin activity were determined.Results:Weiling Granule could decrease the recurrence rate of peptic ulcer,improve injury of the tissue of gastric macosa in the rat,increase gastric wall combining mucus amount,and decrease secretion of gastric acid.Conclusion:Weiling Granule can increase quality of ulcerative healing,which is possibly one of the mechanisms of anti- recurrence of peptic ulcer clinically.