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1.
Article | WPRIM | ID: wpr-835854

ABSTRACT

Objectives@#Loneliness was associated with not only social status but also general health. Psychological conditions in older people have negative effects on general health and oral health. The purpose of the study was to investigate the relationship between loneliness and subjective chewing discomfort in the elderly. @*Methods@#This cross-sectional study analyzed the Korean Social Life, Health, and Aging Project (KSHAP) for the questionnaire, UCLA loneliness scale data of 1,511 older adults living in a rural community. Logistic regression was conducted to identify the relevance of subjective chewing discomfort in the elderly according to the level of loneliness. @*Results@#According to the final model that after adjustment for other risk factors (age, gender, level of education, smoking, drinking, etc.), in the elderly who rarely feel loneliness group compared to the elderly who never feel loneliness was Odds ratio (OR) 1.256 (95% Confidence Interval [CI]: 0.99-1.60) and sometimes+often feel loneliness was OR 2.110 (95% CI: 1.39-3.21). @*Conclusions@#Loneliness is associated with subjective chewing discomfort in the elderly. Older people feeling loneliness are likely to have more subjective chewing discomfort.

2.
Article in Korean | WPRIM | ID: wpr-820818

ABSTRACT

OBJECTIVES: The purpose of this study was to evaluate the association between the distribution of periodontopathic bacteria and oral conditions.METHODS: Stimulated saliva was collected from 162 adults aged 60 years and above. The prevalence and amount of periodontopathic bacteria in the saliva were analyzed using real-time PCR. Pocket depth and clinical attachment loss were examined to evaluate the oral conditions of the subjects. Patients who had at least one tooth surface involved, with a pocket depth ≥4 mm or clinical attachment loss ≥5 mm were classified as having periodontal disease.RESULTS: The detection rates of most bacteria in the orange and green complexes were more than 90%, while those of P. gingivalis and T. forsythia in the red complex were 58.6% and 61.7%, respectively. The number of bacteria in the red complex positively correlated with each other. There were no significant differences in the number of types of red complex bacteria in the saliva and the distribution of residual number of teeth and periodontal disease (P>0.05). On the other hand, the number of remaining teeth in subjects with higher bacterial density were more than those with lesser bacterial density. In addition, the occurrence of dental disease differed significantly depending on the number of P. gingivalis and T. forsythia.CONCLUSIONS: The relative amount of periodontopathic bacteria is important in the occurrence of periodontal disease, and the number of these bacteria positively correlated with each other.


Subject(s)
Adult , Bacteria , Citrus sinensis , Forsythia , Hand , Humans , Oral Health , Periodontal Diseases , Prevalence , Real-Time Polymerase Chain Reaction , Saliva , Stomatognathic Diseases , Tooth
3.
Article in Korean | WPRIM | ID: wpr-740596

ABSTRACT

OBJECTIVES: The purpose of this study was to investigate the job satisfaction level of the dental staff working at oral health centers for people with disabilities. METHODS: A questionnaire survey was conducted with 73 dentists and dental hygienists working at seven regional oral health centers for people with special needs and dental hospitals for people with disabilities in Seoul. The questionnaire consisted of seven questions across two subscales: general satisfaction (4 questions) and satisfaction with wage and welfare (3 questions). The internal consistency of the questionnaire items was assessed using Cronbach's alpha (0.80). The responses were analyzed using a t-test with SPSS (version 23.0). RESULTS: Of the 73 dental staff members, 50% were dentists, 64% were women, and participants' average age was 30 years. In total, 58% of the participants had up to five years of experience working with people with disabilities, 42% of them worked full-time, and each participant treated an average of 200 patients with disabilities per month. The participants reported that their salary was relatively low. Dental hygienists had higher satisfaction level than dentists in the institution's welfare work. The more full-time workers answered, the more suitable they are for their work. CONCLUSIONS: The job satisfaction level of most professionals working in oral health centers for people with disabilities was not very high but they felt rewarded by their welfare work. It was inferred that it is necessary to examine and improve institutional support aspects such as human resource support. Increasing the number of centers in the central region is also needed.


Subject(s)
Dental Hygienists , Dental Staff , Dentists , Disabled Persons , Female , Humans , Job Satisfaction , Oral Health , Reward , Salaries and Fringe Benefits , Seoul
4.
Article in Korean | WPRIM | ID: wpr-740595

ABSTRACT

OBJECTIVES: The aims of this study were to determine the association between the management of oral health and experiences of oral health education and to investigate the influential factors of the Patient Hygiene Performance (PHP) index in an area's university students. In addition, we explored the factors that influence the PHP index and how to effectively promote oral health. METHODS: All collected data were analyzed using the χ2 test, the t-test, and multiple regression using SPSS version 23.0 for Microsoft Windows. The statistical significance level was set at 5%. RESULTS: The total age of 380 participants ranged from 18 to 39 years (mean 22.22±2.43). No significant differences were found in oral health status and experiences of oral health education. CONCLUSIONS: Active national-based policies to develop regular and systematic national oral health education programs are needed.


Subject(s)
Education , Health Behavior , Health Education, Dental , Humans , Hygiene , Oral Health , Oral Hygiene
5.
Article in Korean | WPRIM | ID: wpr-764719

ABSTRACT

OBJECTIVES: The purpose of this study is to determine methods of dental caries prevention by investigating the use of compounds of Diospyros kaki (D. kaki) peel, Momordica charantia (M. charantia), and Canavalia gladiata (C. gladiata) extracts to limit the cariogenic traits of Streptococcus mutans (S. mutans), such as their ability to proliferate and adhere to the tooth surface. METHODS: Broth microdilution and the agar spreading assay were used to determine the antimicrobial effect and minimum inhibitory concentration (MIC) of S. mutans extracts. In order to identify the adhesive ability of S. mutans at varying concentrations, culture plates were first stained with 1 ml of 0.01% crystal violet for 15 minutes at room temperature, and then eluted with 1 ml of EtOH:Acetone (8:2) solution for 15 minutes in a 37℃ incubator. Eluted solutions were then evaluated by use of a spectrophotometer at 575 nm. RESULTS: Experiments were conducted in order to investigate the effectiveness of D. kaki peel, M. charantia, and C. gladiata extracts on limiting the proliferation of S. mutans. The MIC was measured as an indication of whether the antibacterial activity of D. kaki peel, M. charantia, and C. gladiata extracts had a significant bacteriostatic effect on S. mutans. M. charantia extract was effective for growth inhibition on S. mutans at a minimum concentration of 0.25%. From the adhesion ability assay, M. charantia extract had an anti-adhesive effect. CONCLUSIONS: These results indicate that M. charantia extract demonstrates antibacterial activity and has an anti-adhesive effect on S. mutans. Due to these properties, M. charantia extract may be used to prevent dental caries.


Subject(s)
Adhesives , Agar , Canavalia , Dental Caries , Diospyros , Gentian Violet , Incubators , Microbial Sensitivity Tests , Momordica charantia , Momordica , Streptococcus mutans , Streptococcus , Thiram , Tooth
6.
Yonsei Medical Journal ; : 554-560, 2019.
Article in English | WPRIM | ID: wpr-762079

ABSTRACT

PURPOSE: In general, the prevalence of metabolic syndrome (MS) and tooth loss increases with age. We investigated the relationship between the presence of MS, its elements, and tooth loss in middle-aged Korean adults. MATERIALS AND METHODS: This study included Korean adults between 30 and 64 years of age who resided in the capital area of Seoul. From January to June 2014, individuals interested in participating in the oral health survey among those who visited the university hospital's cardiovascular center and provided informed consent were selected. Among 748 subjects who responded to the oral health questionnaires, 30 were excluded due to unclear responses; therefore, a total of 718 were included in the final analysis. RESULTS: The crude odds ratio (OR) of ≥one MS component affecting tooth loss was 1.45 [95% confidence interval (CI), 1.06–2.00]. After adjusting for sex, age, education, income level, occupation, smoking status, kidney disease, chronic obstructive pulmonary disease, and rheumatic disease, the adjusted OR was 1.47 (95% CI, 1.06–2.05), which was statistically significant (p<0.05). The OR for tooth loss was higher in the presence of ≥one component of MS (50–64 years of age) in females. CONCLUSION: This study suggests that female aged 50–64 years may have higher likelihood of tooth loss upon the presence of at least one MS component. Prevention against MS among female of older age could contribute to maintenance of remaining teeth. Further well-designed studies are needed.


Subject(s)
Adult , Education , Female , Humans , Informed Consent , Lung Diseases, Obstructive , Middle Aged , Occupations , Odds Ratio , Oral Health , Prevalence , Renal Insufficiency, Chronic , Rheumatic Diseases , Seoul , Smoke , Smoking , Tooth Loss , Tooth
7.
Article in Korean | WPRIM | ID: wpr-786025

ABSTRACT

OBJECTIVES: This study assessed the antifungal effects of neutral electrolysis hydrogen water, an agent known for its oral anti-bacterial effects on the growth of C. albicans in vitro.METHODS: Hydrogen water, denture cleansing agent, and Listerine® were compared to a tap water control. We serially cultured C. albicans KCTC 27239 in SD media to analyze the inhibitory effect of each treatment on growth. We evaluated the data using repeated-measures ANOVA test and one-way ANOVA test, and a significant difference was defined as P < 0.05.RESULTS: Hydrogen water significantly inhibited the growth, but not the biofilm formation, of C. albicans compared to tap water control at 1-, 5-, and 10-minute time points (P < 0.05).CONCLUSIONS: The growth of C. albicans was slower in hydrogen water than in tap water, thereby suggesting an inhibitory effect of hydrogen water. Hydrogen water may, therefore, be utilized as an antibacterial and preventive agent without any harmful effect on denture and oral tissues.


Subject(s)
Biofilms , Candida albicans , Candida , Dentures , Detergents , Electrolysis , Hydrogen , In Vitro Techniques , Water
8.
Article in English | WPRIM | ID: wpr-713894

ABSTRACT

PURPOSE: Glioblastoma multiforme (GBM) is the most common adult primary intracranial tumor. The remarkable features of GBM include central necrosis. MicroRNAs (miRNAs) have been considered as diagnostic/prognostic biomarkers for many cancers, including glioblastoma. However, the effect of necrosis on the miRNA expression profile and predicted miRNA-mRNA regulatory information remain unclear. The purpose of this study is to examine the effect of necrotic cells on the modulation of miRNA and mRNA expression profiles and miRNA-mRNA network in CRT-MG cells. MATERIALS AND METHODS: We used human astroglioma cells, CRT-MG, treated with necrotic CRT-MG cells to examine the effect of necrosis on the modulation of miRNA and mRNA by next-generation sequencing. For preparation of necrotic cells, CRT-MG cells were frozen and thawed through cycle of liquid nitrogen–water bath. The putative miRNA-mRNA regulatory relationship was inferred through target information, using miRDB. RESULTS: The necrotic cells induced dysregulation of 106 miRNAs and 887 mRNAs. Among them, 11 miRNAs that had a negative correlation value of p < 0.05 by the hypergeometric test were screened, and their target mRNAs were analyzed by Gene Ontology enrichment analysis. Using the Kyoto Encyclopedia of Genes and Genomes database, we also found several necrotic cell treatment-activated pathways that were modulated by relevant gene targets of differentially expressed miRNAs. CONCLUSION: Our result demonstrated that dysregulation of miRNA and mRNA expression profiles occurs when GBM cells are exposed to necrotic cells, suggesting that several miRNAs may have the potential to be used as biomarkers for predicting GBM progression and pathogenesis.


Subject(s)
Adult , Astrocytoma , Baths , Biomarkers , Gene Ontology , Genome , Glioblastoma , Humans , MicroRNAs , Necrosis , RNA, Messenger
9.
Article in English | WPRIM | ID: wpr-740570

ABSTRACT

OBJECTIVES: With the growing elderly population, there is an increasing interest in the oral and general health of elderly individuals. Loss of teeth is representative of oral disease in elderly individuals and is associated with medical and dental problems. The purpose of this study was to investigate the relationship between the number of remaining functional teeth and cognitive function. METHODS: A total of 456 (111 public health centers, 261 senior centers, 84 sanatoriums) older adults (aged ≥65 years) residing in Korea were included. A mental health nurse examined the cognitive function using the Korean version of the Mini-Mental State Examination. A dentist examined the number of functional teeth and denture status through an oral examination, while a dental hygienist surveyed the subjective masticatory level using a questionnaire. RESULTS: The mean age of the participants was 79.5 years (range 65–97 years), and 76.1% of them were women. Participants with a small number of functional teeth had lower cognitive function. In these participants, the odds ratio with poor cognitive function was 2.30 times higher; it was 2.74 times higher after adjusting for age, sex, residence, education, and denture use, and was statistically significant. CONCLUSIONS: Our study suggested that the number of functional teeth was associated with cognitive function in the Korean elderly population.


Subject(s)
Adult , Aged , Cognition , Dental Hygienists , Dentists , Dentures , Diagnosis, Oral , Education , Female , Humans , Korea , Mental Health , Odds Ratio , Public Health , Senior Centers , Tooth
10.
Article in Korean | WPRIM | ID: wpr-194235

ABSTRACT

OBJECTIVES: The aim of this study was to investigate the relationship between cognitive function and occlusal status in elderly individuals. METHODS: A total of 162 individuals aged 65 years and older, who attended the senior citizen center in Daegu city, were included after consent for participation in the study was obtained. The Korean version of the Mini-Mental State Examination (MMSE-K) was used to evaluate the level of cognitive function. Occlusal status was determined using the T-scan III® system. All collected data were analyzed by χ² test, t-test, one way ANOVA, and linear regression analysis using SPSS version 23.0 for Windows. RESULTS: There was a significant relationship between the cognitive function and the use of dentures. Individuals with posterior occlusal status had higher MMSE-K scores than those with anterior occlusal status. There was a positive correlation between the cognitive function and posterior occlusal force. CONCLUSIONS: These results suggest that occlusal status was associated with cognitive function in the elderly individuals. Active national policies to improve occlusal condition in the elderly population are needed.


Subject(s)
Aged , Bite Force , Cognition , Dentures , Humans , Linear Models
11.
Article in Korean | WPRIM | ID: wpr-207261

ABSTRACT

OBJECTIVES: The objectives of this study were to investigate the changes in bisphenol A (BPA) concentrations in urine from before to after sealant filling and the relationship between BPA and the number of teeth with sealed surfaces. METHODS: Thirty-one children aged 6 and 7 years from three elementary schools in Daegu city who did not have any sealant and resin filling were selected as subjects. Urine samples were collected before and after sealant filling until 24 hours, with informed consent from their caregivers. The BPA concentration in all the collected urine samples was analyzed at Seegene Medical. Statistical analysis was performed using the Friedman test, Scheirer-Ray-Hope test, and the repeated-measures generalized linear mixed model of SPSS version 22.0. RESULTS: The BPA concentrations increased from 3.49-μg/g creatinine before to 4.91-μg/g creatinine 2-3 hours later and to 4.15-μg/g creatinine after 24 hours. The more teeth with sealed surfaces, the higher the BPA concentration in children, but the difference was not statistically significant (P>0.05). CONCLUSIONS: The BPA concentrations were highest at 2–3 hours after sealant filling and decreased at 24 hours. Exposure to the sealant appears to have a meaningful correlation with the concentration of BPA in the urine of children.


Subject(s)
Caregivers , Child , Creatinine , Humans , Informed Consent , Tooth
12.
Article in Korean | WPRIM | ID: wpr-207259

ABSTRACT

OBJECTIVES: Stress is a psychological reaction to stimuli such as anxiety or threat felt by a person either physically or mentally when placed in a difficult situation. Although a relationship between stress and dry mouth has been reported, it remains understudied. The purpose of this study was to investigate the association between stress and subjective dry mouth among the elderly living in a rural region. METHODS: A total of 214 people aged ≥60 years living in a rural area were recruited with an informed consent for a cross-sectional analysis. Information about stress and subjective dry mouth was obtained by an interview survey with a constructed questionnaire. Data on other potential confounding factors (including oral factors) such as socio-demographic data, health-related behaviors, the number of remaining teeth, and subjective chewing ability were also gathered at the same time. Logistic regression models were used to analyze the relationship of stress and dry mouth using SPSS. RESULTS: The Crude odds ratio (OR) of stress awareness affecting subjective dry mouth was 2.59 (95% confidence interval: 1.43–4.68). After adjusting for sex, education, income, smoking, and alcohol intake, the adjusted OR was 2.52 (95% confidence interval: 1.30–4.87) which was statistically significant. CONCLUSIONS: Elderly people who were stressed had an approximately 2-fold increase in experiencing subjective dry mouth when compared to their stress-free counterparts.


Subject(s)
Aged , Anxiety , Cross-Sectional Studies , Education , Humans , Informed Consent , Logistic Models , Mastication , Mouth , Odds Ratio , Pilot Projects , Smoke , Smoking , Tooth
13.
Article in English | WPRIM | ID: wpr-207255

ABSTRACT

OBJECTIVES: Unmet needs for dental treatment are one of the potential contributing factors to poor oral health because oral health problems worsen if left untreated. This study aimed to demonstrate the prevalence of and the causes for unmet dental needs, and to evaluate the association between unmet needs for dental treatment and oral health status. METHODS: Data on 3,883 subjects aged ≥18 years from the Korean National Oral Health Survey 2006 were analyzed. Information regarding unmet needs for dental treatment was obtained using standardized questionnaires. Eight trained dentists examined decayed, missing, or filled teeth (DMFT). Multiple regression models were built to assess the association between unmet needs for dental treatment and the DMFT scores. RESULTS: The prevalence of perceived unmet needs for dental treatment was 34.7% among the adult Korean population. Economic constraints were the main cause (38.6%) for unmet dental needs. The average DMFT scores were higher in the subjects with unmet needs for dental treatment than in those without. In individuals with unmet needs for dental treatment within the past 1 year, the number of decayed teeth after adjusting for confounders was likely to be greater by 0.58 and that of missing teeth by 0.27 compared to that in their counterparts with no unmet dental needs in the past 1 year. CONCLUSIONS: Perceived unmet needs for dental treatment were significantly associated with poor oral health status among the adult Korean population. Further studies are needed to clarify the direct and indirect effects of unmet needs for dental treatment on an individual's oral health status by investigating critical variables of the causal pathways among perceived dental needs, dental care utilization, and oral health status.


Subject(s)
Adult , Dental Care , Dental Health Surveys , Dentists , DMF Index , Health Services Needs and Demand , Humans , Oral Health , Prevalence , Tooth
14.
Article in English | WPRIM | ID: wpr-728761

ABSTRACT

Glioblastoma multiforme (GBM) is the most common primary intracranial tumor in adults and has poor prognosis. The GBM-specific tumor microenvironment (TME) plays a crucial role in tumor progression, immune escape, local invasion, and metastasis of GBM. Here, we demonstrate that hypoxia, reactive oxygen species (ROS), and differential concentration of glucose influence the expression of cytokines and chemokines, such as IL-6, IL-8, and IP-10, in human glial cell lines. Treatment with cobalt chloride (CoCl₂) and hydrogen peroxide (H₂O₂) significantly increased the expression levels of IL-6, IL-8, and IP-10 in a dose-dependent manner in CRT-MG and U251-MG astroglioma cells, but not in microglia cells. However, we found strikingly different patterns of expression of cytokines and chemokines between H₂O₂-treated CRT-MG cells cultured in low- and high-glucose medium. These results suggest that astroglioma and microglia cells exhibit distinct patterns of cytokine and chemokine expression in response to CoCl₂ and H₂O₂ treatment, and different concentrations of glucose influence this expression under either hypoxic or oxidant-enriched conditions.


Subject(s)
Adult , Hypoxia , Astrocytoma , Cell Line , Chemokines , Cobalt , Cytokines , Glioblastoma , Glucose , Humans , Hydrogen Peroxide , Hydrogen , Interleukin-6 , Interleukin-8 , Microglia , Neoplasm Metastasis , Neuroglia , Prognosis , Reactive Oxygen Species , Tumor Microenvironment , United Nations
15.
Article in Korean | WPRIM | ID: wpr-19270

ABSTRACT

OBJECTIVES: Dental caries and periodontitis are major oral bacterial infections associated with dental plaque. Infection and inflammation play a role in carcinogenesis, and a significant link has been found between some highly prevalent oral and dental diseases and some types of cancer. Therefore, the aim of this study was to evaluate the relationship between cancer and oral health among Korean adults. METHODS: Data from the 6th Korea National Health and Nutrition Examination Survey were used to analyze the incidence of cancer according to oral health. Demographic and socioeconomic statuses and oral behavior of the participants was analyzed using complex chi-square tests. Logistic regression was used to analyze the relationship between a high DMFT index and cancer by calculating the 95% confidence intervals. RESULTS: In total, 6,450 case-control subjects were included. Significant difference was observed in the DMFT index, but not in periodontal diseases, between the cases and controls. The odds ratio for cancer was 1.80 (95% confidence interval: 1.18 to 2.73) in the high-risk group. However, no significant difference was observed after adjusting for age, education level, and income. CONCLUSIONS: The results provide potential evidence of a significant association between cancer and oral disease.


Subject(s)
Adult , Bacterial Infections , Carcinogenesis , Case-Control Studies , Dental Caries , Dental Plaque , Education , Humans , Incidence , Inflammation , Korea , Logistic Models , Nutrition Surveys , Odds Ratio , Oral Health , Periodontal Diseases , Periodontitis , Social Class , Stomatognathic Diseases
16.
Article in Korean | WPRIM | ID: wpr-156071

ABSTRACT

OBJECTIVES: Many recent studies have suggested that several systemic conditions, such as obesity, hypertension, hyperlipidemia, and diabetes, are related to periodontitis. The aim of this study was to investigate whether any particular metabolic syndrome component correlates with the periodontal status. METHODS: This study analyzed data from 501 adults (age range, 30 to 64 years) who completed a general physical examination and an oral-health-related questionnaire between January 2014 and June 2014. Periodontitis was already used by the validity and reliability has been proven questionnaire. Multiple logistic regression analysis was used to assess the association between metabolic syndrome and the presence of chronic periodontitis. RESULTS: Among the 501 participants, 81 (16.2%) had metabolic syndrome and 91 (18.2%) had chronic periodontitis. The prevalence of chronic periodontitis was 28.40% (23/81) and 16.19% (68/420) in the participants with and without metabolic syndrome, respectively. The association remained significant after adjusting for sex, age, education, income, occupation, and smoking status; the adjusted odds ratio was 2.03 (95% confidence interval, 1.11 to 3.71). CONCLUSIONS: A significant association exists between metabolic syndrome components and the periodontal status.


Subject(s)
Adult , Chronic Periodontitis , Education , Humans , Hyperlipidemias , Hypertension , Logistic Models , Obesity , Occupations , Odds Ratio , Periodontitis , Physical Examination , Prevalence , Reproducibility of Results , Smoke , Smoking
17.
Yonsei Medical Journal ; : 1494-1499, 2016.
Article in English | WPRIM | ID: wpr-143161

ABSTRACT

PURPOSE: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. MATERIALS AND METHODS: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. RESULTS: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. CONCLUSION: Conclusion: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.


Subject(s)
Apoptosis , Blotting, Western , Caspase 3 , Cell Death , Cell Survival , Colon , Colonic Neoplasms , Necrosis , Photochemotherapy , Propidium
18.
Yonsei Medical Journal ; : 1494-1499, 2016.
Article in English | WPRIM | ID: wpr-143156

ABSTRACT

PURPOSE: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. MATERIALS AND METHODS: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. RESULTS: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. CONCLUSION: Conclusion: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.


Subject(s)
Apoptosis , Blotting, Western , Caspase 3 , Cell Death , Cell Survival , Colon , Colonic Neoplasms , Necrosis , Photochemotherapy , Propidium
19.
Article in Korean | WPRIM | ID: wpr-50018

ABSTRACT

OBJECTIVES: Many people rely on recommendations from family, relatives, and other information sources in order to select the appropriate dental clinic for treatment. The object of this study was to find out the relationship between quality of dental service and Korean Net Promoter Score (KNPS). METHODS: A total of 520 patients were selected through consecutive sampling from four dental clinics in Daegu city. Informed consent of all selected patients was obtained. The patients were required to complete a questionnaire that comprised of three categories of queries related to provision of satisfactory dental service, relation quality, and personal data. Sub-categories of questions included physical and environmental factors, human service factors, patients' satisfaction with related quality and KNPS. Data was collected by interviewing all individuals on a personal basis over a period of 3 months. Statistical analysis was performed using studental t-test, ANOVA and multiple regression analysis using the SPSS 20.0 software (IBM Co., Armonk, NY, USA). RESULTS: Among the various socio-economic factors studied, income was most significantly related to KNPS while satisfaction of relation quality was the largest factor affecting the KNPS. Factors such as satisfactory physical environment, quality of human service, relation quality and income accounted for 52.4% of KNPS. In short, the three major factors that affected KNPS were patient compliance, professionalism of the attending dentist and response of hygienist to patient. Other minor factors influencing KNPS included trust upon the attending dentist, aesthetics of clinicstiinterior, and physical convenience. CONCLUSIONS: It is highly recommended that dental clinics should make strenuous efforts to increase the level of patient compliance, professionalism, and response towards patients, which can be achieved by improving the quality of service and through positive marketing of the hospital.


Subject(s)
Dental Clinics , Dentists , Esthetics , Humans , Informed Consent , Marketing , Patient Compliance , Professionalism
20.
Article in Korean | WPRIM | ID: wpr-655325

ABSTRACT

The purposes of this study were to investigate the factors affection the Oral Impacts on Daily Performances for Children (C-OIDP) in elementary and middle school students, and identify the association between oral health-related behaviors, oral health condition and C-OIDP. A cross-sectional study was conducted in three schools in Incheon, Asan, Korea. A total of 175 selected children were interviewed by a trained examiner using a questionnaire. Oral Health Related Quality of Life was assessed by the Korean version of C-OIDP. Socio-economic characteristics, oral health-related behaviors, oral health condition and C-OIDP were verified using the questionnaire. ANOVA analysis was performed to determine the oral health and C-OIDP, and multiple regression analysis was performed to determine the factors affecting the C-OIDP. The activities with the greatest effect were eating (28.0%), cleaning teeth (22.9%), and smiling (18.9%). In the logistic regression model, the high item score of C-OIDP was associated with experiencing dental caries and gum pain in the past month. The more the C-OIDP prevalence item, the more the fillng deciduous tooth surface (fs) (p=0.024), caries experienced deciduous tooth surface (dfs) (p=0.049), total caries tooth surface (ds+DS) (p=0.021), and total caries experienced tooth surface (dfs+DMFS) (p=0.047). It can be concluded that the factors affecting C-OIDP are fs, dfs, dfs+DMFS, and gingival pain. Based on these results, we can improve C-OIDP to advance preventive practice.


Subject(s)
Child , Cross-Sectional Studies , Dental Caries , Eating , Gingiva , Humans , Korea , Logistic Models , Oral Health , Prevalence , Quality of Life , Smiling , Tooth , Tooth, Deciduous
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