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1.
Acta Pharmaceutica Sinica ; (12): 605-614, 2022.
Article in Chinese | WPRIM | ID: wpr-922903

ABSTRACT

Proteasome controls the degradation of proteins closely related to life activities and plays a key role in the maintenance of protein homeostasis. Proteasome activities decrease with aging, followed by the overwhelming production of damaged proteins which far exceed the protein consumption. Accumulation of these proteins leads to various diseases including neurodegenerative diseases. Therefore, inducing toxic protein degradation is considered as a promising solution for the treatment of these diseases, while increasing the activity of proteasome is considered as an important strategy. However, the research in this field is still in the preliminary stage, and this review will focus on the discussion of the research progress of various small molecule proteasome activators, including research methods, pharmacological effects, structure-activity relationships and the existing problems.

2.
Article in English | WPRIM | ID: wpr-892408

ABSTRACT

Objective@#: A role of diffusion-weighted imaging (DWI) in the diagnosis of cerebral venous thrombosis (CVT) is not wellunderstood. This study evaluates the effectiveness of DWI in the diagnosis of CVT. @*Methods@#: Literature search was conducted in electronic databases for the identification of studies which reported the outcomes of patients subjected to DWI for CVT diagnosis. Random-effects meta-analyses were performed to achieve overall estimates of important diagnostic efficiency indices including hyperintense signal rate, the sensitivity and specificity of DWI in diagnosing CVT, and the apparent diffusion coefficient (ADC) of DWI signal areas and surrounding tissue. @*Results@#: Nineteen studies (443 patients with 856 CVTs; age 40 years [95% confidence interval (CI), 33 to 43]; 28% males [95% CI, 18 to 38]; symptom onset to DWI time 4.6 days [95% CI, 2.3 to 6.9]) were included. Hyperintense signals on DWI were detected in 40% (95% CI, 26 to 55) of the cases. The sensitivity of DWI for detecting CVT was 22% (95% CI, 11 to 34) but specificity was 98% (95% CI, 95 to 100). ADC values were quite heterogenous in DWI signal areas. However, generally the ADC values were lower in DWI signal areas than in surrounding normal areas (mean difference−0.33×10-3 mm2/s [95% CI, −0.44 to −0.23]; p<0.00001). @*Conclusion@#: DWI has a low sensitivity in detecting CVT and thus has a high risk of missing many CVT cases. However, because of its high specificity, it may have supporting and exploratory roles in CVT diagnosis.

3.
Article in Chinese | WPRIM | ID: wpr-882663

ABSTRACT

Objective:To investigate the role of blood ammonia in the evaluation of the prognosis of septic patients in the emergency department and to compare its value with mortality in emergency department sepsis (MEDS) score.Methods:A retrospective clinical study was conducted to septic patients who were diagnosed in the Emergency Department of West China Hospital of Sichuan University from June 2017 to May 2018, and met the diagnostic criteria established by the diagnostic criteria of the American College of Chest Physicians/Society of Critical Care Medicine in 2001. The subjects who had other diseases that affected blood ammonia level and were lost to follow-up were excluded. MEDS scores were collected, and the survival status of patients was followed up by telephone. The independent samples t test was used to compare the differences between the two groups, receiver operating characteristic (ROC) curve was used to assess the accuracy of the prediction of sepsis mortality, and the logistic regression model was used to explore the value of the combined use of blood ammonia and MEDS score.Results:Eighty subjects were finally included in the study and divided into the 1-week survival group ( n=52), 1-week death group ( n=28); 4-week survival group ( n=37), 4-week death group ( n=43); 12-week survival group ( n=33), 12-week death group ( n=47); 1-year survival group ( n=32), and 1-year death group ( n=48). There was no statistical difference in the demographic characteristics of subjects between the groups. The average blood ammonia level of all the subjects who died was higher than that of the patients who survived in the same period [(116.57 ± 85.33) μmol/L vs (77.63 ± 35.82) μmol/L, (108.53 ± 73.00) μmol/L vs (71.19 ± 32.53) μmol/L, (106.74 ± 71.59) μmol/L vs (69.21 ± 28.84) μmol/L, (105.77 ± 71.14) μmol/L vs (69.50 ± 29.25) μmol/L, P<0.05]. Based on death after one week, four weeks, twelve weeks and one year, the area under ROC curve (AUC) of blood ammonia was 0.668 (95% CI: 0.542-0.793, P=0.014), 0.706 (95% CI: 0.593-0.819, P=0.002), 0.705 (95% CI: 0.592-0.818, P=0.002), and 0.697 (95% CI: 0.582-0.811, P=0.003), respectively. Compared with the use of blood ammonia, lactic acid or MEDS score alone, the combined use of blood ammonia and MEDS score increased the accuracy of prognosis evaluation in sepstic patients ( P<0.05). Conclusions:Blood ammonia has a high value in predicting the short-term and 1-year prognosis of septic patients in the emergency department. The combined use of blood ammonia and MEDS score can further improve its predictive value.

4.
Article in Chinese | WPRIM | ID: wpr-882612

ABSTRACT

Objective:To explore the clinical effect of Huayu-Ningkun liquid enema and external application of dreg for treating chronic pelvic inflammatory disease. Methods:A total of 106 patients with chronic pelvic inflammatory disease who met the inclusion criteria in the gynecological outpatient department of Beijing Hospital of Integrated Chinese and Western Medicine from January 2018 to December 2019 were divided into 2 groups by random number table method, with 53 patients in each group. The control group was treated with conventional western medicine therapy, and the observation group was combined with Huayu-Ningkun liquid enema and external application of drug residue on the basis of the control group. TCM symptom scores were performed before and after treatment, and the Quality of Life of patients was evaluated by Generic Quality of Life Inventory-74 (GQOLI-74). Serum CRP and TNF-α levels were detected by ELISA, and adverse reactions during treatment were recorded. Results:The total effective rate was 96.2% (51/53) in the observation group and 83.0% (44/53) in the control group, and the difference between the two groups was statistically significant ( χ2=4.970, P=0.026). After treatment, the TCM symptom scores of the observation group was significantly lower than that of the control group ( t=8.205, P<0.01). The scores of physical function, mental function, material life and social function were significantly higher than those in the control group ( t value were 7.503, 8.786, 7.798, 9.353, all Ps<0.001). After treatment, the levels of serum CRP (4.52 ± 3.46 g/L vs. 8.23 ± 3.35 g/L, t=5.608) and TNF-α (1.00 ± 0.35 μg/L vs. 1.52 ± 0.28 μg/L, t=14.551) in the observation group were significantly lower than those in the control group ( P<0.01). Conclusion:The Huayu-Ningkun liquid enema and drug residue external application combined with western medicine can relieve the symptoms, reduce the level of inflammatory cytokines, improve clinical efficacy of the patients with chronic pelvic inflammatory disease.

5.
Journal of Clinical Hepatology ; (12): 2324-2326, 2021.
Article in Chinese | WPRIM | ID: wpr-904941

ABSTRACT

Objective To investigate HBV RNA level in patients with HBV-related hepatocellular carcinoma after long-term antiviral therapy and its clinical significance. Methods A total of 60 patients with HBV-related hepatocellular carcinoma who were admitted to Tianjin Second People's Hospital from June 2019 to August 2020 were enrolled in this study. These patients received antiviral therapy with nucleos(t)ide analogues (NAs) for at least two years, and high-sensitivity HBV DNA detection showed a HBV RNA level of < 20 IU/mL at least twice at an interval of 3 months. Liver function, HBV serum markers, and HBV RNA level were measured for all patients. The Kruskal-Wallis H test was used for comparison between multiple groups, and the Wilcoxon rank-sum test was used for comparison between two groups; a Pearson correlation analysis was used to investigate the influencing factors for HBV RNA. Results Among the 60 patients with HBV-related hepatocellualr carcinoma who received long-term antiviral treatment, 9 (15%) tested positive for HBV RNA. According to the level of alpha-fetoprotein (AFP), the patients were divided into AFP positive group and AFP negative group, and there was no significant difference in HBV RNA level between the two groups [0(0-3.57) vs 0(0-2.00), Z =-1.474, P =0.141). According to Barcelona Clinic Liver Cancer (BCLC) stage, they were divided into BCLC stage A group and BCLC stage B+C+D group, and there was no significant difference in HBV RNA level between the two groups [0(0-2.0) vs 0(0-2.0), Z =-0.607, P =0.544]. According to HBeAg level, the patients were divided into HBeAg positive group and HBeAg negative group, and there was a significant difference in HBV RNA level between the two groups [2.99(0-4.80) vs 0(0-0.50), Z =-3.400, P =0.001]. According to the titer of HBsAg, they were divided into HBsAg≤100 IU/mL group, 100 IU/mL < HBsAg < 1500 IU/mL group, and HBsAg ≥1500 IU/mL group, and there was a significant difference in HBV RNA level between the three groups [0(0-0.0) vs 0(0-0.20) vs 2.00(0.0-4.54), H =-7.899, P =0.019]. A Pearson correlation analysis was performed for age, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, alkaline phosphatase, alpha-fetoprotein, HBsAg, and HBeAg, and the results showed that HBsAg level was correlated with HBV RNA quantification ( r =0.292, P < 0.05). Conclusion In patients with HBV-related hepatocellualr carcinoma receiving long-term antiviral therapy with NAs, HBV RNA can still be detected after HBV DNA is lower than the lower limit of detection. HBsAg titer may be correlated with serum HBV RNA level.

6.
Journal of Clinical Hepatology ; (12): 2324-2326, 2021.
Article in Chinese | WPRIM | ID: wpr-904891

ABSTRACT

Objective To investigate HBV RNA level in patients with HBV-related hepatocellular carcinoma after long-term antiviral therapy and its clinical significance. Methods A total of 60 patients with HBV-related hepatocellular carcinoma who were admitted to Tianjin Second People's Hospital from June 2019 to August 2020 were enrolled in this study. These patients received antiviral therapy with nucleos(t)ide analogues (NAs) for at least two years, and high-sensitivity HBV DNA detection showed a HBV RNA level of < 20 IU/mL at least twice at an interval of 3 months. Liver function, HBV serum markers, and HBV RNA level were measured for all patients. The Kruskal-Wallis H test was used for comparison between multiple groups, and the Wilcoxon rank-sum test was used for comparison between two groups; a Pearson correlation analysis was used to investigate the influencing factors for HBV RNA. Results Among the 60 patients with HBV-related hepatocellualr carcinoma who received long-term antiviral treatment, 9 (15%) tested positive for HBV RNA. According to the level of alpha-fetoprotein (AFP), the patients were divided into AFP positive group and AFP negative group, and there was no significant difference in HBV RNA level between the two groups [0(0-3.57) vs 0(0-2.00), Z =-1.474, P =0.141). According to Barcelona Clinic Liver Cancer (BCLC) stage, they were divided into BCLC stage A group and BCLC stage B+C+D group, and there was no significant difference in HBV RNA level between the two groups [0(0-2.0) vs 0(0-2.0), Z =-0.607, P =0.544]. According to HBeAg level, the patients were divided into HBeAg positive group and HBeAg negative group, and there was a significant difference in HBV RNA level between the two groups [2.99(0-4.80) vs 0(0-0.50), Z =-3.400, P =0.001]. According to the titer of HBsAg, they were divided into HBsAg≤100 IU/mL group, 100 IU/mL < HBsAg < 1500 IU/mL group, and HBsAg ≥1500 IU/mL group, and there was a significant difference in HBV RNA level between the three groups [0(0-0.0) vs 0(0-0.20) vs 2.00(0.0-4.54), H =-7.899, P =0.019]. A Pearson correlation analysis was performed for age, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, alkaline phosphatase, alpha-fetoprotein, HBsAg, and HBeAg, and the results showed that HBsAg level was correlated with HBV RNA quantification ( r =0.292, P < 0.05). Conclusion In patients with HBV-related hepatocellualr carcinoma receiving long-term antiviral therapy with NAs, HBV RNA can still be detected after HBV DNA is lower than the lower limit of detection. HBsAg titer may be correlated with serum HBV RNA level.

7.
Article in English | WPRIM | ID: wpr-900112

ABSTRACT

Objective@#: A role of diffusion-weighted imaging (DWI) in the diagnosis of cerebral venous thrombosis (CVT) is not wellunderstood. This study evaluates the effectiveness of DWI in the diagnosis of CVT. @*Methods@#: Literature search was conducted in electronic databases for the identification of studies which reported the outcomes of patients subjected to DWI for CVT diagnosis. Random-effects meta-analyses were performed to achieve overall estimates of important diagnostic efficiency indices including hyperintense signal rate, the sensitivity and specificity of DWI in diagnosing CVT, and the apparent diffusion coefficient (ADC) of DWI signal areas and surrounding tissue. @*Results@#: Nineteen studies (443 patients with 856 CVTs; age 40 years [95% confidence interval (CI), 33 to 43]; 28% males [95% CI, 18 to 38]; symptom onset to DWI time 4.6 days [95% CI, 2.3 to 6.9]) were included. Hyperintense signals on DWI were detected in 40% (95% CI, 26 to 55) of the cases. The sensitivity of DWI for detecting CVT was 22% (95% CI, 11 to 34) but specificity was 98% (95% CI, 95 to 100). ADC values were quite heterogenous in DWI signal areas. However, generally the ADC values were lower in DWI signal areas than in surrounding normal areas (mean difference−0.33×10-3 mm2/s [95% CI, −0.44 to −0.23]; p<0.00001). @*Conclusion@#: DWI has a low sensitivity in detecting CVT and thus has a high risk of missing many CVT cases. However, because of its high specificity, it may have supporting and exploratory roles in CVT diagnosis.

8.
Acta Pharmaceutica Sinica ; (12): 29-36, 2021.
Article in Chinese | WPRIM | ID: wpr-872612

ABSTRACT

Depression is a serious mental illness with a high incidence. At present, we do not fully understand the specific pathological mechanisms of depression, and the efficacy of drug treatments is very limited. Recent studies have shown that epigenetic changes that occur in specific brain regions may be a key mechanism by which environmental factors to interact with individuals to influence the risk of depression. Therefore, drugs that target epigenetic regulation may become a new direction for the development of antidepressants. Histone deacetylase inhibitors (HDACi) are a class of compounds that inhibit histone deacetylase activity, which has been reported to be associated with depression; this article addresses the use of HDACi in preclinical studies, and their potential therapeutic role and limitations of use in depression.

9.
Article in Chinese | WPRIM | ID: wpr-906084

ABSTRACT

Objective:To study the protective effect of the Wenyang Huoxue Huatan prescription (WYHXHT) on cardiotoxicity induced by adriamycin. Method:SD rats were randomly divided into the following six groups: a normal control group, an adriamycin model group, a low-dose (4.86 g·kg<sup>-1</sup>) WYHXHT group, a middle-dose (9.72 g·kg<sup>-1</sup>) WYHXHT group, a high-dose (19.44 g·kg<sup>-1</sup>) WYHXHT group, and a dexrazoxane group. Except for the normal control group, the rats in other groups received intraperitoneal injection of 2.5 mg·kg<sup>-1</sup> adriamycin, once a week for six weeks, with a cumulative dose of 15 mg·kg<sup>-1</sup>. The normal control group, the adriamycin model group, and the dexrazoxane group received 10 mL·kg<sup>-1</sup> normal saline daily by gavage. In the dexrazoxane group, the rats were subjected to intraperitoneal injection of 25 mg·kg<sup>-1</sup> dexrazoxane 30 min before doxorubicin administration, once a week for six weeks. The general condition of rats was observed and their body weight was monitored. A high-resolution micro-ultrasound imaging system was used to detect rat cardiac function. Hematoxylin-eosin (HE) staining was performed to observe the pathological changes of myocardial tissues of rats. Western blot was used to detect the protein expression of microtubule-associated protein 1 light chain 3 (LC3) Ⅱ, the mammalian homolog of yeast Atg6 (Beclin-1), and p62 protein in rat myocardial tissues. Result:Compared with the normal control group, rats in the adriamycin model group showed dull fur, reduced food intake and activity, loose stool, low energy, and slow response. Besides, it also displayed reduced body weight (<italic>P</italic><0.01), decreased left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) (<italic>P</italic><0.01), myocardial cell degeneration, edema, rupture, and dissolution, expansion of myocardial interstitium, uneven staining of myocardial fiber, visible inflammatory cell infiltration, up-regulated expression of Beclin-1 and LC3Ⅱ in rat myocardial tissues (<italic>P</italic><0.01), and down-regulated p62 expression (<italic>P</italic><0.01). Compared with the adriamycin model group, the medium- and high-dose WYHXHT groups exhibited increased body weight, LVEF, and LVFS (<italic>P</italic><0.01), relieved pathological injury of myocardial tissues, down-regulated expression of LC3Ⅱ and Beclin-1 (<italic>P</italic><0.05,<italic>P</italic><0.01), and up-regulated expression of p62 (<italic>P</italic><0.05,<italic>P</italic><0.01). Conclusion:WYHXHT can effectively prevent and treat adriamycin-induced cardiotoxicity, and its effect may be related to the inhibition of myocardial cell autophagy. The effect is dominant in the high-dose group.

10.
Article in Chinese | WPRIM | ID: wpr-905271

ABSTRACT

Objective:To explore the factors affecting curative effect of motor imagery brain-computer interface (MI-BCI) training on upper limb paralysis for subacute stroke patients. Methods:From January, 2018 to July, 2019, 23 inpatients with post-stroke upper limb paralysis accepting MI-BCI training were reviewed. The gender, age, course of disease, aphasia, location and nature of lesion, history of Botulinum toxin, hemisphere injured and modified Ashworth Scale (MAS) score of affected fingers were recorded, and they were assessed with Fugl-Meyer Assessment-Upper Extremities (FMA-UE) before and four weeks after MI-BCI training. According to improvement of FMA-UE wrist and hand scores (≥ 2), the patients were divided into effective group (n = 11) and inefficacy group (n = 12). Results:The MAS scores before MI-BCI training (t = 2.677, P < 0.05) and history of botulinum toxin (Z = 0.000, P < 0.05) were more in the inefficacy group than in the efficacy group. FMA-UE scores (total and dimensions) after training were correlated to their baseline levels (r > 0.831, P < 0.01), FMA-UE total scores (Eta = 0.453, P < 0.05) and upper arms scores (Eta = 0.506, P < 0.05) were correlated to aphasia, FMA-UE scores of hands were correlated with MAS (r = -0.521, P < 0.05). Conclusion:Poor baseline motor function, spasticity and complication with aphasia were the factors unfavorable to MI-BCI training for subacute stroke patients with upper limb paralysis.

11.
China Pharmacy ; (12): 952-960, 2021.
Article in Chinese | WPRIM | ID: wpr-876265

ABSTRACT

OBJECTIVE:To stud y the effects of sinapine thiocyanate (ST) on the proliferation ,epithelial mesenchymal transformation(EMT)and metastasis of human cutaneous squamous cell carcinoma SCL- 1 cells,and to investigate its possible mechanism. METHODS :Human cutaneous squamous cell carcinoma SCL- 1 cells were divided into blank control group (0.1% DMSO) and ST different concentration groups (5,10,20 μmol/L). CCK- 8 assay,5-ethynyl-2′-deoxyuridine(EDU)test, scratch test and Transwell chamber invasion test were adopted to test the proliferation ,migration and invasion ability. The expression of N-cadherin and E-cadherin were detected by Western blot and immunofluorescence assay . Other SCL- 1 cells were collected and divided into blank control group (0.1% DMSO),ST group (20 μmol/L),ST+NSC228155 group [ 20 μmol/L ST+100 μmol/L NSC228155(EGFR agonist )] and ST+SC 79 group [ 20 μmol/L ST+20 μmol/L SC79(PI3K/Akt agonist )]. The proliferation ,migration and invasion ability of SCL- 1 cells in each group were detected by CCK- 8 assay,scratch test and Transwell chamber invasion assay. The expression of epidermal growth factor receptor (EGFR),phosphatidylinositol 3 kinase(PI3K),phosphorylated phosphatidylinositol 3 kinase(p-PI3k),protein kinase B (Akt)and phosphorylated protein Akt (p-Akt)protein of cells in blank control group and ST different concentration groups(5,10,20 μmol/L)were determined by Western blot assay so as to validate the relationship between ST effect and EGFR/ PI3K/Akt signaling pathway. SCL- 1 cells and human normal skin fibroblasts cell WS 1 were divided into blank control group (0.1% DMSO),ST group (20 μmol//L),ZD1839 group(positive control ,20 μmol//L,EGFR inhibitor )and LY 294002 group(positive control,20 μmol//L,PI3K/Akt inhibitor ). CCK- 8 assay was used to detect the cell proliferation in order to evaluate the cells cytotoxicity of ST. RESULTS :Compared with blank control group ,the proliferation ,migration and invasion ability of SCL- 1 cells were significantly decreased in 5,10,20 μmol/L ST groups(P<0.05). Western blot and immunofluorescence assay showed that the expression of N-cadherin in SCL- 1 cells were decreased significantly in 5,10,20 μmol/L ST groups(P<0.05),while the protein expression of E-cadherin was increased significantly (P<0.05);the protein expressions of EGFR ,p-PI3K and p-Akt were significantly decreased (P<0.05). Compared with ST group ,the proliferation ,migration and invasion ability of SCL- 1 cells were increased significantly in ST + NSC 228155 group and ST + SC 79 group (P<0.05). Compared with blank control group ,the proliferation ability of WS 1 cells had no significant change in ST group ,while the proliferation ability of SCL- 1 cells was decreased significantly (P<0.05);the proliferation ability of the two kinds of cells were decreased significantly in ZD 1839 group and LY 294002 group(P<0.05). Compared with ST group ,the proliferation ability of WS 1 cells was decreased significantly in ZD1839 group and LY 294002 group(P<0.05),but there was no significant difference in the proliferation ability of SCL- 1 cells (P>0.05). CONCLUSIONS :ST may inhibit the proliferation ,EMT and metastasis of SCL- 1 cells through inhibiting the activation of EGFR/PI 3K/Akt signaling pathway ,and its side effects are few.

12.
Article in English | WPRIM | ID: wpr-880652

ABSTRACT

OBJECTIVES@#To analyze the effect of hyperlipoproteinemia (α) on immediate expansion after coronary stent implantation guided by intravascular ultrasound (IVUS).@*METHODS@#A total of 160 patients (175 lesions) with coronary heart disease diagnosed by coronary artery angiography, who were performed percutaneous intervention guided by IVUS in the Department of Cardiology, Third Xiangya Hospital, Central South University, were enrolled retrospectively.According to the concentration of lipoproteina, the patients were divided into 2 groups: a hyperlipoproteinemia (α) group and a control group. Cardiac ejection fraction was measured with echocardiography. Logistic regression was used to analyze the influential factors for hyperlipoproteinemia (α). The target vessel was examined by IVUS to analyze the immediate expansion effect of hyperlipoproteinemia (α) after stent implantation.@*RESULTS@#The mean stent expansion index, lesion length, stent number, stent symmetry index and posterior balloon diameter were (94.73±18.9)%, (52.92±29.1) mm, (2.11±0.85), (83.62±13.07)%, and (9.46±2.00) mm in the hyperlipoproteinemia (α) group, respectively. Compared with the control group, there were significantly difference (all @*CONCLUSIONS@#Hyperlipoproteinemia (α) appears to be a predictor of stent underexpansion, and the decreased creatinine clearance rate is an independent risk factor for hyperlipoproteinemia (α).


Subject(s)
Coronary Angiography , Coronary Artery Disease/surgery , Humans , Lipoproteins , Percutaneous Coronary Intervention/adverse effects , Retrospective Studies , Risk Factors , Stents/adverse effects , Treatment Outcome , Ultrasonography, Interventional
13.
Article in Chinese | WPRIM | ID: wpr-911645

ABSTRACT

Objective:To explore the efficacy and safety of simultaneous pancreas-kidney (SPK) transplantation in patients aged over 60 years.Methods:A retrospective analysis was performed for 150 SPK patients from January 1, 2013 to June 30, 2019. Based upon age, they were divided into three groups of ≥60 years ( n=21), 50-60 years ( n=44) and <50 years ( n=85). Clinical data of three groups were compared, including postoperative rejection, perioperative graft thrombosis, reoperative frequency, average hospitalization time and readmission ratio. And cardiocerebrovascular complications before/after-SPK, CMV viremia within 1 year post-SPK, fasting blood glucose, fasting C-peptide, fasting insulin level, HbA1c at 1 year post-SPK, glomerular filtration rate (eGFR) at 1 year post-SPK and survival rate of patient/graft were compared. Results:There were 21 cases in ≥60 years group, accounting for 14% of the total number of cases and the maximal age was 67 years. The proportion of preoperative cardiovascular events was 14.3%(3/21) in ≥60 years group, 34.1%(15/44) in 50-60 years group and 7.1%(6/85) in <50 years group. Statistical difference existed among three groups ( P=0.001). A pairwise comparison indicated that preoperative cardiovascular event in 50-60 years group was higher than that in <50-years group ( P=0.0006). The postoperative cardiovascular events in three groups were 4.8%, 4.5% and 2.4% respectively and there was no statistical difference ( P=0.537). The incidence of graft thrombosis in three groups was 2 cases (9.5%) in ≥60 years group, 1 case (2.3%) in 50-60 years group and 7 cases (8.2%) in <50 years group ( P=0.384). The proportion of reoperation in three groups was 14.3%, 18.3% and 18.8% respectively and there was no statistical difference ( P=0.889). The causes of death were cerebral hemorrhage ( n=2), myocardial infarction ( n=2) and tumor ( n=1); ≥ 60 years group ( n=1), 50-60 years group ( n=1) and <50 years group ( n=3). No significant difference existed among three groups ( P=0.842). There was no significant difference in average postoperative hospitalization time, readmission rate, postoperative rejection, postoperative 1-year CMV viremia, postoperative cerebrovascular events, postoperative 1-year fasting blood glucose, fasting C-peptide, fasting insulin level, HbA1c, postoperative 1-year eGFR or patient/graft survival rate among three groups. Conclusions:Through strict preoperative evaluations, SPK for patients aged over 60 years increases no operative risk and achieves the same outcome.

14.
Acta Pharmaceutica Sinica ; (12): 1754-1761, 2020.
Article in Chinese | WPRIM | ID: wpr-825160

ABSTRACT

italic>N-Acetylaspartate (NAA) is a highly abundant brain metabolite. Nowadays, as an important marker reflecting the function of nervous system, NAA is widely used in the results analysis of nuclear magnetic resonance spectroscopy (1H MRS). NAA is synthesized in mitochondria of neurons and metabolized in oligodendrocytes. Additionally, NAA may be converted to the dipeptide N-acetylaspartylglutamate (NAAG), and catabolized into NAA and glutamate in astrocytes. NAA is related to a variety of central nervous system diseases, including Canavan disease, multiple sclerosis, depression, schizophrenia and other mental diseases. Therefore, NAA may be a biomarker of these diseases, and its related enzymes may be used as therapeutic targets for drug screening. Here, we combined the current research on the molecular mechanisms of NAA to reveal the process of NAA generation, metabolism and transport in the brain, explain the possible physiological effects of NAA and discuss its relationship with central nervous system diseases, explore the prospect of NAA in disease prediction and diagnosis, as well as the targeted treatment that may become the breakthrough of refractory diseases.

15.
Article in Chinese | WPRIM | ID: wpr-863800

ABSTRACT

Objective:To investigate the role of epidemiological history in the screening of corona virus disease 2019 (COVID-19) in fever clinic, to improve the efficiency in fever clinic and reduce the incidence of cross infection.Methods:This is a retrospective study. Patients who were admitted to the Fever Clinic in West China Hospital of Sichuan University from January 23th, 2020 to February 11th, 2020 were included in the study. According to epidemiological history, the patients were divided into the epidemiological history group (experimental group) and no epidemiological history group (control group). The two groups of patients were admitted and treated separately. The clinical data, NEWS score, etiology results, viral pneumonia showed on CT, time of visit, COVID-19 patient ratio, and admission composition ratio were compared between the two groups. The measurement data were presented as the mean ± standard deviation (SD), and the numeration data were expressed as ratio or constituent ratio. The measurement data of normal distribution between the two groups were compared by independent sample t test. The measurement data of skewed distribution were expressed by the median (interquartile range), and the comparison between the two groups was tested by non-parameter. The differences between enumeration data were assessed by chi-square test. A P<0.05 was considered statistically significant. Results:A total of 2423 patients were included, including 927 patients in the experimental group and 1296 patients in the control group. There were no significant differences in gender, NEWS score and clinical symptoms between the two groups ( P> 0.05). The age (35.00 ± 12.80 vs 38.13 ± 15.57 years) , the proportion of fever patients (28.80% vs 32.75%) and waiting time (31.72 vs 58.08 min) of the experimental group were lower than those of the control group, and the difference was statistically significant (P <0.05). The CT examination ratio (37.54% vs 20.39%), viral pneumonia ratio shown on CT (9.77% vs 2.95%), ratio of examined COVID-19 nucleic acid test (85.44% vs 56.75%), and the admission ratio (16.72% vs 9.63%) of the experimental group were higher than those of the control group, and the differences were statistically significant (P <0.05). There was no significant difference in the positive rates of influenza virus and rhinovirus between the two groups ( P> 0.05). Conclusion:It is necessary to adjust the management mode of fever clinic during the corona virus disease 2019, and to manage the patients according to the epidemiological history which can improve the screening efficiency and reduce the risk of cross infection.

16.
Article in Chinese | WPRIM | ID: wpr-863792

ABSTRACT

Objective:To analysis the clinical characteristics of The clinical characteristics of using automated external defibrillation in the public place,To explore the feasibility and effectiveness of AED application in public places in China.Methods:From January 2014 to April 5, 2019, 54 cases of on-site emergency medical records of AED use in public places in China were analyzed retrospectively from three aspects: patient and AED user attributes, and AED clinical performance.Results:After field application of AED analysis, 54 patients did not have out of hospital cardiac arrest in 9 patients; cardiac arrest in 45 patients, cerebral resuscitation in 40 patients (88.9%), death in 5 patients (11.1%), one of them died in hospital. The accuracy of AED for defibrillation rhythm recognition and defibrillation recommendations was 100%. The success rate of shock to VF was 97.22%, and that of non pulse VT was 100%. The data shows that AEDs of different brands show clinical effectiveness in the core indicators of work. The operation level of the rescuer determines the critical time of AED shock, which is closely related to the prognosis of the patient ( P<0.05) . Conclusions:AED is reliable and effective in electric shock decision and performance.The overall efficiency of AED application can be improved by strengthening training, shortening the critical time of electric shock, rational configuration and effective management.

17.
Article in Chinese | WPRIM | ID: wpr-849648

ABSTRACT

Objective To investigate the effect of breast conserving surgery in early triple negative breast cancer (TNBC). Methods Data of 56 early (stage and Ⅱ) TNBC patients in the Eighth Medical Center of PLA General Hospital from January 2010 to January 2015 were analyzed retrospectively, and they were divided into breast-conserving surgery group (n=27) and modified radical surgery group (n=29) who chose the modified radical surgery due to their own willingness and economic factors. Patients in both groups received adjuvant chemoradiotherapy and were followed up for 5 years. Postoperative local recurrence rate, distal metastasis rate, 5-year survival rate, operative time, amount of bleeding, hospital stays, postoperative complications, Symptom Check List 90 (SCL90) were compared between the two groups. Results There were no significant differences in local recurrence rate, distant metastasis rate, operative time and survival rate between the breast-conserving surgery group and the modified radical surgery group (P>0.05). The postoperative complications, amount of bleeding, hospital stays in the breast-conserving surgery group were significantly lower than those in the modified radical surgery group (P<0.05). The SCL90 total score, total symptom index, positive items, and average of positive items of the patients who survived 5 years after the operation were also significantly lower than those of the modified radical surgery group (P<0.05). Conclusion Breast-conserving surgery for early TNBC patients is safe and effective. There was no significant difference in operation time between breast-conserving surgery and modified radical surgery, but breast-conserving surgery can shorten the postoperative recovery time, reduce surgical complications, and improve the postoperative quality of life.

18.
Article in Chinese | WPRIM | ID: wpr-872723

ABSTRACT

Objective:To investigate the therapeutic effect and possible mechanism of four types of Chinese herbal moisturizers made in laboratory for atopic dermatitis induced by 2,4-dinitrofluorobenzene (DNFB) in mice. Method:According to the body weight, BALA/c mice were randomly divided into normal group, model group, blank cream group (moisturizer A), Shaoyao Gancaotang group (moisturizer B), Shaoyao Gancaotang with Portulacae Herba,Ginseng Radix et Rhizoma and Honeysuckle Stem group (moisturizer C), and Shaoyao Gancaotang with Ginseng Radix et Rhizoma and Honeysuckle Stem group (moisturizer D) , with the dose of 25 g·kg-1 per day, as well as tacrolimus ointment group of 3 g·kg-1 per day, with 10 to 12 mice in each group. Except the normal group, the mice in the other groups were treated with 0.5% DNFB in the hair removal skin of back, 100 μL each for 7 days. Starting from the 7th day, each group was given the appropriate skin cream for external use intervention, once per day, for 15 consecutive days, except for the normal and the model groups. The animal body mass was measured once a week, and the animal back skin was graded three times a week, and the skin lesion score was recorded. After the mice were killed, the left and right ears were taken, the weight of both ears was punched and the degree of swelling was calculated. The back skin was fixed and stained with hematoxylin-eosin(HE) method, and then pathologic examination was conducted to observe and score the pathological changes of mouse back skin. Blood was obtained after the last dose and enzyme-linked immunosorbent assay (ELISA) was used to determine the immunoglobulin(Ig)E content in serum. Western blot was used to measure the expression of signal transduction and activator of transcription 3 (STAT3), phosphorylation (p)-STAT3 in the skin tissue. Result:Compared with the normal group, the body mass decreased continuously, a series of inflammatory changes such as erythema, edema, dryness, desquamation and callus exfoliation and so on occurred in the modeling area, and the skin lesion score increased significantly in the model group. Additionally, the cuticle of ear skin was thickened and the degree of ear swelling was obviously increased in the model group. Microscopically, the occurred changes in the model mice included the local necrosis of the epidermis, epidermal thickening, epidermal hyperplasia, and the hyperkeratosis and hypokeratosis in the cuticle, as well as the subcutaneous inflammatory cell infiltration and so on. Furthermore, the content of serum IgE andthe expression of p-STAT3 in skin tissues increased significantly in the model group. Compared with the model group, the body mass of mice in group C and D was significantly increased (P<0.01), and the skin lesion status score was decreased (P<0.05,P<0.01).The degree of auricle swelling was significantly reduced in group B, C and D compared with that in the model group (P<0.01).The degree of skin necrosis and defect and epidermal hyperplasia of mice in moisturizer C group was significantly reduced compared with that in model group (P<0.05,P<0.01). Serum IgE levels of mice in group C and D were significantly lower than those in the model group (P<0.05,P<0.01). The expression of p-STAT3 protein in skin tissues of mice in moisturizer C group was significantly lower than that in model group (P<0.05). Conclusion:The moisturizers B, C and D all have certain therapeutic effect on atopic dermatitis, among which moisturizers C has the most obvious therapeutic effect. The possible mechanism may be that it reduces the level of inflammatory cytokines by inhibiting the increase of serum IgE content and the phosphorylation of STAT3.

19.
Chinese Journal of Geriatrics ; (12): 365-368, 2020.
Article in Chinese | WPRIM | ID: wpr-869372

ABSTRACT

The exact etiology and pathogenesis of Parkinson's disease(PD)are currently unknown.With intensifying research on PD, it has been found that Helicobacter pylori(H.pylori)infection is closely related to PD.In a hypoxic environment, the gastric mucosa is in a state of chronic hypoxia, which increases the likelihood of gastric mucosal damage and H. pylori infection and may be one of the factors affecting the gastrointestinal symptoms of PD and leading to the deterioration of PD.This article reviews recent advances on the correlation between H. pylori infection and PD in hypoxia.

20.
Article in Chinese | WPRIM | ID: wpr-878669

ABSTRACT

Objective To explore the clinical characteristics of relapsing polychondritis(RP)patients presented with arthropathy. Methods We retrospectively analyzed the clinical data of 201 RP patients who were hospitalized in our center between December 2005 and February 2019.After 16 patients with co-existing other autoimmune diseases and malignancies were ruled out,185 RP patients entered the final analysis,among whom 16 RP patients were presented with arthropathy and 169 without arthropathy.The demographic data,clinical manifestations,laboratory findings,and prognosis were compared between these two groups. Results Five of the 16 RP patients with arthropathy at presentation were misdiagnosed as rheumatoid arthritis.Compared with RP patients without arthropathy at presentation,RP patients with arthropathy at presentation had a longer disease course[(37.50±66.50)months


Subject(s)
Arthritis, Rheumatoid , Delayed Diagnosis , Diagnostic Errors , Humans , Joint Diseases/diagnosis , Polychondritis, Relapsing/diagnosis , Prognosis , Retrospective Studies
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