Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 135
Filter
1.
Article in Chinese | WPRIM | ID: wpr-940386

ABSTRACT

ObjectiveTo study the virulence and biofilm inhibition effect of Fufang Huangbai Fluid Paint (FFHBFP) on methicillin-resistant Staphylococcus aureus (MRSA), and to explore the antibacterial effect of FFHBFP on MRSA, which provides a theoretical basis and reference for clinical medication. MethodFirstly, the microdilution method and time–growth curve were used to determine the minimum inhibitory concentration (MIC) of FFHBFP and vancomycin (VAN) against MRSA and the effect on bacterial growth. The effects of FFHBFP and VAN on the inhibition of MRSA virulence factor lipase and restoration of hydrogen peroxide (H2O2) sensitivity were detected under sub-minimum inhibitory concentration (sub-MIC). The inhibitory effect of FFHBFP and VAN on MRSA biofilm formation and maturation was detected by the microplate method. The morphological changes of mature biofilms before and after administration were observed under a scanning electron microscope (SEM). Real-time polymerase chain reaction (Real-time PCR) was utilized to detect the effect of 50.600 g·L-1 concentration of FFHBFP on the expression of MRSA virulence gene crtM and biofilm-forming genes fnbA and icaA. Finally, molecular docking technology was used to predict the mechanism of potential antibacterial active ingredients of FFHBFP in inhibiting the virulence and biofilm of MRSA. ResultThe MIC of VAN was 2 mg·L-1, and VAN below 1 mg·L-1 exerted no effect on MRSA growth. The MIC of FFHBFP was not determined, while the 101.200-202.400 g·L-1 original solution inhibited MRSA growth. Compared with the blank group and the VAN group, sub-MIC (25.300-50.600 g·L-1 original solution) inhibited lipase and recovered MRSA sensitivity to H2O2 (P<0.01). The results of the microplate method showed that FFHBFP (25.300-202.400 g·L-1 original solution) inhibited biofilm formation and maturation (P<0.05, P<0.01). The SEM exhibited that FFHBFP made the structure of biofilm loose and the size of the bacteria varied. FFHBFP at 50.600 g·L-1 concentration can inhibit the expression of related virulence genes and biofilm-forming genes (P<0.05, P<0.01), and molecular docking results also showed that the main antibacterial active ingredients in FFHBFP have good binding ability to the target. ConclusionFFHBFP that cannot directly kill MRSA exerts clinical efficacy by impairing virulence expression, biofilm formation, and other pathogenic properties.

2.
Article in Chinese | WPRIM | ID: wpr-933914

ABSTRACT

Objective:To summarize the prenatal diagnosis and postnatal follow-up of 15q11.2 BP1-BP2 microdeletion syndrome (Burnside-Butler syndrome, BBS), and provide a reference for the management of BBS.Methods:A retrospective analysis was performed on 27 singleton pregnancies with fetal BBS that were prenatally diagnosed by single nucleotide polymorphism(SNP) array of amniotic fluid in Wuxi Maternity and Child Health Care Hospital from January 2017 to September 2021. Prenatal diagnosis indications, serological screening, prenatal ultrasound features, SNP array results, and postnatal growth and development were described and summarized.Results:(1) Of the 27 cases, the indications of prenatal diagnosis in 14 cases were abnormal sonographic findings, including eight cases with increased nuchal translucency, two with cleft lip and palate/alveolar process cleft, one with fetal multiple joint contracture syndrome, one with fetal right diaphragmatic hernia and single umbilical artery, one with suspected fetal duodenal atresia and one with nasal bone absence. Other indications included high risk of Down syndrome by serological screening in six cases, history of adverse pregnancy in six cases, and advanced age in one case. (2) Karyotyping of amniotic fluid in these 27 BBS fetuses showed normal results and SNP array indicated the deletion range of 311.8-855.3 kb. Parental verification of 23 cases confirmed one was a new mutation, seven were inherited from the father and 15 from the mother. (3) Five pregnancies were terminated in the second trimester and the remaining 22 cases were live births. (4) The median follow-up of the 22 children was 1 year 8 months (range 0.5 months to 4 years 3 months), which found low body weight and/or growth retardation in six cases, low body weight with language retardation in one case, low body weight with growth retardation and hyperactive behavior in one case, language retardation with left ear appendage in one case, cleft palate accompanied by duodenum/cleft lip and alveolar cleft in two cases without abnormal development after surgical treatment, and no abnormal growth in the remaining 11 cases.Conclusion:For BBS fetuses, the proportion of ultrasound abnormalities is high but with a low specificity in prenatal diagnosis, and the risk of abnormal postnatal growth and development/behavior is high, which requires continuous monitoring.

3.
Article in Chinese | WPRIM | ID: wpr-933902

ABSTRACT

Objective:To investigate the ultrasonographic and genetic features of Cri-du-chat syndrome (CDCS).Methods:In this retrospective study, cases with CDCS diagnosed in Wuxi Maternal and Child Health Care Hospital from 2004 to 2021 and with complete data were reviewed to describe and analyze the maternal serum prenatal screening, non-invasive prenatal testing (NIPT), ultrasound, genetic examination data, and pregnancy outcomes.Results:All cases were diagnosed by karyotype analysis, seven of them were diagnosed prenatally through amniotic fluid, and four were diagnosed after birth through peripheral blood. Five of the seven cases diagnosed prenatally had an abnormal serological screening, including two cases with 5p- indicated by NIPT. Of the 11 cases, prenatal ultrasonography showed cerebellar transverse diameter less than -2 SD in eight cases, including four with cerebellar hypoplasia (CH), two with fetal growth restriction, and two with cranial diameters less than -2 SD. One case was shown with an increased nuchal translucency, accompanying bilateral choroid plexus cysts of the lateral ventricles, and suspected persistent left superior vena cava. No obvious ultrasound abnormality was observed in the remaining two cases. Among the seven cases diagnosed prenatally, excluding one case that refused parental verification, further single nucleotide polymorphism array (SNP array) showed that all six cases inherited the de novo mutations from the parents. The cytogenetic analysis found the breakpoints at 5p13, 5p14, and 5p15 in five, three, and three cases. All seven pregnancies were terminated in the second trimester. Four children diagnosed postnatally presented with CDCS phenotype during the follow-up at three years old. Conclusions:Fetal CDCS should be considered with CH detected by prenatal ultrasonography, though the correlation between CH and CDCS still needs further investigation. Gene mapping with an SNP array is helpful for phenotypic profiling and genetic counseling.

4.
Article in Chinese | WPRIM | ID: wpr-931440

ABSTRACT

Objective:To construct the curriculum content system of Extreme Environmental medicine based on the trainees' competency.Methods:After analysis of Chinese doctors' post competency demand, and the characteristics of military students, qualitative and quantitative methods were used to construct the curriculum system. The consistency of expert opinions was represented by Kendall's W coefficient, using chi-square test. The hierarchy and weights of all items were analyzed by the analytic hierarchy process, using consistency ratio ( CR) , which was incorporated into this system after passing the test ( CR<0.1) . Results:The selective experts were all authoritative and positivity, the assessments were consistency. Finally, it formed 5 primary items, 15 secondary items, and 54 third items of educational materials and 13 knowledge modules and 1 comprehensive seminar.Conclusions:Based on the demand of Chinese doctors' post competency, a curriculum system of extreme environment medicine has been constructed, through combined application of qualitative and quantitative research methods.

5.
Article in English | WPRIM | ID: wpr-928590

ABSTRACT

Multisystem inflammatory syndrome in children (MIS-C) is a type of hyperinflammatory symptoms similar to Kawasaki disease after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and is commonly observed in children aged 8-10 years. Primary therapeutic medications for MIS-C are intravenous immunoglobulins and glucocorticoids. It has been reported that biologics, such as IL-1 receptor antagonist anakinra, IL-6 receptor antagonist tocilizumab, and TNF-α receptor antagonist infliximab, can be used as an option for critically ill patients. This article elaborates on the mechanism of action of the above biologics and discusses the efficacy and safety biologics in the treatment of MIS-C after SARS-CoV-2 infection, in order to provide methods for the treatment of MIS-C with severe symptoms.


Subject(s)
Biological Products , COVID-19/complications , Child , Humans , SARS-CoV-2 , Systemic Inflammatory Response Syndrome
6.
Acta Physiologica Sinica ; (6): 125-133, 2022.
Article in English | WPRIM | ID: wpr-927588

ABSTRACT

Captopril can have nephrotoxic effects, which are largely attributed to accumulated renin and "escaped" angiotensin II (Ang II). Here we test whether angiotensin converting enzyme-1 (ACE1) inhibition damages kidneys via alteration of renal afferent arteriolar responses to Ang II and inflammatory signaling. C57Bl/6 mice were given vehicle or captopril (60 mg/kg per day) for four weeks. Hypertension was obtained by minipump supplying Ang II (400 ng/kg per min) during the second 2 weeks. We assessed kidney histology by periodic acid-Schiff (PAS) and Masson staining, glomerular filtration rate (GFR) by FITC-labeled inulin clearance, and responses to Ang II assessed in afferent arterioles in vitro. Moreover, arteriolar H2O2 and catalase, plasma renin were assayed by commercial kits, and mRNAs of renin receptor, transforming growth factor-β (TGF-β) and cyclooxygenase-2 (COX-2) in the renal cortex, mRNAs of angiotensin receptor-1 (AT1R) and AT2R in the preglomerular arterioles were detected by RT-qPCR. The results showed that, compared to vehicle, mice given captopril showed lowered blood pressure, reduced GFR, increased plasma renin, renal interstitial fibrosis and tubular epithelial vacuolar degeneration, increased expression of mRNAs of renal TGF-β and COX-2, decreased production of H2O2 and increased catalase activity in preglomerular arterioles and enhanced afferent arteriolar Ang II contractions. The latter were blunted by incubation with H2O2. The mRNAs of renal microvascular AT1R and AT2R remained unaffected by captopril. Ang II-infused mice showed increased blood pressure and reduced afferent arteriolar Ang II responses. Administration of captopril to the Ang II-infused mice normalized blood pressure, but not arteriolar Ang II responses. We conclude that inhibition of ACE1 enhances renal microvascular reactivity to Ang II and may enhance important inflammatory pathways.


Subject(s)
Angiotensin II/pharmacology , Animals , Arterioles/metabolism , Captopril/pharmacology , Hydrogen Peroxide/pharmacology , Kidney , Mice
7.
Article in Chinese | WPRIM | ID: wpr-882593

ABSTRACT

Objective:To investigate the effect of Chaishao-Shugan-Lidan-Paishi Decoction assisted with laparoscopic cholecystectomy in the treatment of chronic cholecystitis with gallstones. Methods:According to the random number table method, 198 cases of chronic cholecystitis combined with cholecystolithiasis were admitted to our hospital from January 2016 to January 2019 and were divided into two groups, 99 cases in each group. Laparoscopic cholecystectomy was performed in both group. Then the control group was treated with Ursodeoxycholic Acid, and the observation group was treated with Chaishao-Shugan-Lidan-Paishi Decoction on the basis of the control group. Both groups were treated for 8 weeks. The clinical efficacy was evaluated after the treatment TCM symptoms and signs were scored before and after the treatment; the levels of TNF-α, IL-6, hs-CRP, gastrin (GAS), motilin (MOT) and somatostatin (SST) were detected by double antibody sandwich ELISA, and the adverse reactions during the treatment were recorded. Results:The total effective rate of the observation group was 94.9% (94/99), the control group was 82.8% (82/99), the difference between the two groups was statistically significant ( χ2=7.364, P=0.007). The score of the right flank pain, the level of upset and irritable condition, the sense of fullness of epigastric cavity, greasiness, nausea and vomiting, belching score after the treatment in the observation group were significantly lower than those in the control group ( t values were 7.229, 4.773, 4.442, 2.302, 5.887, 4.235, respectively, P<0.05 or P<0.01). After the treatment, the levels of serum TNF-α, IL-6, hs-CRP in the observation group were significantly lower than those in the control group ( t values were 9.330, 18.425, 26.886, respectively, all Ps<0.05), and the levels of serum GAS (104.53 ± 2.13 ng/L vs. 96.61 ± 3.29 ng/L, t=20.106) and MOT (386.60 ± 9.42 ng/L vs. 345.50 ± 12.80 ng/L, t=25.731) in the observation group were significantly higher than those of the control group, SST (40.05 ± 4.39 ng/L vs. 48.42 ± 5.11 ng/L, t=12.362) in the observation group was significantly lower than that of the control group ( P<0.01). No serious adverse reactions occurred during the treatment period in both groups. Conclusion:Chaishao-Shugan-Lidan-Paishi Decoction assisted laparoscopic cholecystectomy in the treatment of chronic cholecystitis with gallstones can improve the clinical symptoms, inhibit inflammation, improve gastrointestinal function, improve clinical efficacy.

8.
Chinese Journal of Trauma ; (12): 1090-1098, 2021.
Article in Chinese | WPRIM | ID: wpr-909981

ABSTRACT

Objective:To compare the efficacy of metaphyseal expansion and non-expansion when using proximal femoral nail antirotation(PFNA)in the treatment of severe osteoporotic intertrochanteric fracture in the elderly.Methods:A retrospective case-control study was conducted to analyze the clinical data of 66 elderly patients with severe osteoporotic intertrochanteric fracture admitted to Honghui Hospital, Xi'an Jiaotong University from January 2019 to January 2020, including 49 males and 17 females. The age ranged from 75 to 89 years[(80.9±3.3)years]. The AO types of fractures were 31-A1 in 23 patients, 31-A2 in 22 and 31-A3 in 21. A total of 34 patients were stabilized by the PFNA technique with metaphyseal expansion(metaphyseal expansion group)and 32 patients received PFNA fixation without metaphyseal expansion(metaphyseal non-expansion group). The total blood loss, hidden blood loss, intraoperative blood loss, dominant blood loss, postoperative drainage, transfusion volume, transfusion rate, operation time, hospital stay and fracture healing time were compared between the two groups. The hip functional recovery was assessed by Harris hip score at 1, 3, 6 months postoperatively and at the last follow-up. The incidence of complications was observed.Results:All patients were followed up for 12-17 months[(13.5±1.1)months]. The total blood loss, hidden blood loss, intraoperative blood loss, dominant blood loss, postoperative drainage in metaphyseal expansion group were(976.3±210.1)ml,(712.4±224.4)ml,(139.4±21.0)ml,(263.8±36.3)ml,(124.4±29.5)ml respectively, significantly higher than those in metaphyseal non-expansion group[(799.0±119.5)ml,(603.0±136.4)ml,(94.1±18.8)ml,(195.9±35.4)ml,(101.9±27.5)ml]( P<0.05). The transfusion volume[2(0, 2)U]and transfusion rate[53%(18/34)]in metaphyseal expansion group ware increased compared to metaphyseal non-expansion group[0(0, 1.5)U, 25%(8/32)]( P<0.05). There were no significant differences in operation time, hospital stay, fracture healing time or hip Harris score at 1, 3, 6 months after surgery and the last follow-up between the two groups( P>0.05). No wedge-shaped distraction deformity, fracture nonunion, femoral head necrosis, wound infection, pulmonary embolism or fat embolism occurred in both groups. There was no significant difference in the incidence of iatrogenic lateral wall fracture, lower limb venous thrombosis or postoperative medical complications between the two groups( P>0.05). Conclusion:For elderly patients with severe osteoporotic intertrochanteric fracture, both PFNA with metaphyseal expansion and non-expansion can achieve satisfactory results, while the metaphyseal non-expansion is superior in perioperative blood loss and transfusion rate.

9.
Article in Chinese | WPRIM | ID: wpr-906459

ABSTRACT

Objective:To study whether Tanreqing injection (TRQ) can alleviate the body injury in the process of infection by inhibiting the production and release of <italic>α</italic>-hemolysin of <italic>Staphylococcus aureus</italic> under sub-minimal inhibitory concentration, and to provide experimental basis for better guidance of clinical medication. Method:The effects of TRQ on the minimum inhibitory concentration (MIC) and bacterial growth of <italic>S.aureus</italic> were determined firstly by microplate method and time-growth curve. The different sub-minimal inhibitory concentrations of TRQ were co-cultured with bacteria or bacterial supernatants, and then co-incubated with defibrillated rabbit blood to detect the inhibitory and neutralizing effects of TRQ on <italic>S.aureus</italic> <italic>α</italic>-hemolysin. Cell counting kit-8 (CCK-8) cell viability assay was used to detect the protective effect of TRQ on <italic>S. aureus</italic>-mediated damage to human alveolar epithelial cells (A549). Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect the effect of sub-minimal inhibitory concentration of TRQ on the mRNA expression of <italic>S.aureus</italic> <italic>α</italic>-hemolysin regulatory genes hla and agrA. Result:The MIC of TRQ to <italic>S.aureus </italic>was 1/8 of the stock solution, and the sub-minimal inhibitory concentration (1/64MIC-1/16MIC) TRQ used in this study did not affect the growth of bacteria. 1/64MIC-1/16 MIC TRQ had the effect of inhibiting and neutralizing the hemolytic activity of <italic>α</italic>-hemolysin, with a protective effect on <italic>S.aureus</italic> supernatant-mediated A549 cell damage, and its inhibitory effect on <italic>α</italic>-hemolysin was closely related to the inhibition of hla and agrA mRNA expression. Conclusion:The sub-minimal inhibitory concentration TRQ can inhibit and neutralize the hemolytic activity of <italic>α</italic>-hemolysin of <italic>S.aureus</italic>, with a protective effect on A549 cell damage mediated by <italic>S.aureus</italic> infection, and its mechanism of inhibiting <italic>α</italic>-hemolysin is closely related to the interference with agr regulatory system.

10.
Article in Chinese | WPRIM | ID: wpr-906250

ABSTRACT

Objective:To investigate the factors affecting the quality of post-harvest Angelicae Dahuricae Radix (Baizhi) and to provide the corresponding suggestions according to these factors. Method:A series of factors affecting the quality of Baizhi were analyzed comprehensively by visiting several origins, medicinal material markets, pharmacies, supermarkets and storehouses combined with plenty of literature reports. Result:The rot of Baizhi was the most common phenomenon after harvest, and mildew, discoloration and moth could occur during the processes of processing, storage and packaging. So the quality of Baizhi should be controlled strictly through the whole process. The roots of Baizhi should be harvested on sunny days to avoid mining damage and dried as soon as possible to prevent rotting. The drying methods included both traditional lime burial and modern ways such as sulfur fumigation, saline dehydration, sun drying method, and oven drying method. However, the water content should be strictly controlled. Furthermore, the storehouses should be disinfected firstly. The temperature and humidity of the storehouses should be controlled strictly. Some suitable ways could be adopted to prevent moth and discoloration during storage, which included airtight fumigation of aluminum phosphide, cold storage, air conditioning maintenance, antagonism storage with other Chinese medicinal materials, dry ice storage at room temperature, etc. Large quantities of Baizhi were generally packaged in woven bags. Otherwise, kraft paper bags, polyvinyl chloride plastic bags, glass bottles, aluminum foil composite film bags, and other packaging materials had been adopted for the retail. However, the packaging specification should be selected according to the situation. Conclusion:The quality of Baizhi can be directly affected by harvesting, drying, processing, storage and packaging. Scientific and reasonable methods should be adopted to ensure the quality of Baizhi.

11.
Article in English | WPRIM | ID: wpr-922471

ABSTRACT

Human dental pulp stem cells (hDPSCs) are easily obtained multipotent cells, however, their potential value in regenerative medicine is hindered by the phenotypic and functional changes after conventional monolayer expansion. Here, we employed single-cell RNA sequencing (scRNA-seq) to comprehensively study the transcriptional difference between the freshly isolated and monolayer cultured DPSCs. The cell cluster analysis based on our scRNA-seq data showed that monolayer culture resulted in a significant cellular composition switch compared to the freshly isolated DPSCs. However, one subpopulation, characterized as MCAM(+)JAG(+)PDGFRA(-), maintained the most transcriptional characteristics compared to their freshly isolated counterparts. Notably, immunofluorescent staining revealed that the MCAM(+)JAG(+)PDGFRA(-) hDPSCs uniquely located in the perivascular region of human dental pulp tissue. Flow-cytometry analysis confirmed that their proportion remained relatively stable (~2%) regardless of physiological senescence or dental caries. Consistent with the annotation of scRNA-seq data, MCAM(+)JAG(+)PDGFRA(-) hDPSCs showed higher proliferation capacity and enhanced in vitro multilineage differentiation potentials (osteogenic, chondrogenic and adipogenic) compared with their counterparts PDGFRA(+) subpopulation. Furthermore, the MCAM(+)JAG(+)PDGFRA(-) hDPSCs showed enhanced bone tissue formation and adipose tissue formation after 4-week subcutaneous implantation in nude mice. Taken together, our study for the first time revealed the cellular composition switch of monolayer cultured hDPSCs compared to the freshly isolated hDPSCs. After in vitro expansion, the MCAM(+)JAG(+)PDGFRA(-) subpopulation resembled the most transcriptional characteristics of fresh hDPSCs which may be beneficial for further tissue regeneration applications.


Subject(s)
Animals , Cell Differentiation , Dental Caries , Dental Pulp , Humans , Mice , Mice, Nude , Stem Cells
12.
Article in Chinese | WPRIM | ID: wpr-922019

ABSTRACT

OBJECTIVE@#Patients with 22q11.2 microduplications have highly variable clinical phenotypes. The clinical manifestations and prognosis of 19 fetuses carrying 22q11.2 microduplications were analyzed.@*METHODS@#The fetuses were analyzed by single nucleotide polymorphism array (SNP array), which was followed by parental validation. Pregnancy outcome and clinical features of the newborns were analyzed in order to delineate genotype-phenotype correlation.@*RESULTS@#Two fetuses were identified by karyotyping analysis of amniotic fluid samples. SNP array revealed that all have carried a 468.8 kb~3.4 Mb duplication in 22q11.2 region. Two couples have refused parental verification. Seven cases were inherited from the mother, 6 were from the father, and 4 cases were de novo in origin. Three couples opted termination of the pregnancy. One fetus perished at birth. Five newborns showed delayed growth, the remaining 10 were normal.@*CONCLUSION@#The prenatal phenotype of fetuses carrying 22q11.2 microduplications are nonspecific, and the phenotypes of survivors may become more diverse along with increased age. Professional evaluation and long-term follow-up should be recommended.


Subject(s)
Female , Fetus , Genetic Counseling , Humans , Infant, Newborn , Karyotyping , Phenotype , Pregnancy , Prenatal Diagnosis
13.
Article in Chinese | WPRIM | ID: wpr-886757

ABSTRACT

Objective To investigate the population dynamics and Echinococcus infections in small rodents around human settlement in Yushu City, Qinghai Province. Methods Rodents were captured using the mouse trap method in pastures from Batang Township and Longbao Township of Yushu City, Qinghai Province on May, August and October, 2018. The body weight and snout-vent length of all captured rodents were measured, and the species was identified according to the rodent morphology. Genomic DNA was extracted from rodent liver specimens and lesion specimens, and the mitochondrial cox1 gene of Echinococcus was amplified using PCR assay for identification of parasite species. In addition, the tissue specimens positive for PCR assay were sampled for pathological examinations. The prevalence of Echinococcus infections was estimated in rodents, and a phylogenetic tree was created based on Echinococcus cox1 gene sequences. Results A total of 285 small rodents were captured, including 143 Ochotona curzoniae (50.2%), 141 Lasiopodomys fuscus (49.5%), and 1 Neodon irene (0.3%), and there was a remarkable variation in habitat selection among these three rodent species. The number of L. fuscus correlated positively with vegetation coverage (r = 0.350, P = 0.264), with the greatest number seen in August, and the number of O. curzoniae negatively with vegetation coverage (r = −0.371, P = 0.235), with the highest number seen in August and the lowest number in May. The female/male ratios of O. curzoniae and voles were 1:0.96 and 0.82:1, respectively. The body weight (r = 0.519, P < 0.01) and snout-vent length (r = 0.578, P < 0.01) of O. curzoniae showed a tendency towards a rise with month, while the body weight (r = −0.401, P < 0.01) and snout-vent length (r = −0.570, P < 0.01) of voles presented a tendency towards a reduction with month. No Echinococcus infection was detected in voles, while 2.1% prevalence of E. shiquicus infection was seen in O. curzoniae. Phylogenetic analysis revealed consistent sequences of cox1 gene from E. shiquicus in Yushu City of Qinghai Province and Shiqu County, Ganzi Tibetan Autonomous Prefecture of Sichuan Province. Conclusions The small rodents around the human settlement in Yushu City of Qinghai Province mainly include O. curzoniae and L. fuscus, with the greatest numbers seen in May and August, respectively. Following the concerted efforts for echinococcosis control, the prevalence of Echinococcus infections is low in small rodents around the human settlement in Yushu City; however, there is still a risk of echinococcosis transmission.

14.
Article in Chinese | WPRIM | ID: wpr-885047

ABSTRACT

The data of pediatric patients who required sedation for non-invasive examinations, aged 1 day-13 yr, were retrospectively collected from December 2019 to June 2020.They are divided into 4 groups according to age: newborn (age 0-28 days) group, infant (age 29 day-1 yr) group, toddler (age 1-3 yr) group, and child (age 4-13 yr) group.Chloral hydrate 50 mg/kg was administrated orally.When the Ramsay score was ≤ 4 points at 30 min after administration, oral chloral hydrate 25 mg/kg or intranasal midazolam 0.1 mg/kg or intranasal dexmedetomidine 1 μg/kg was given as rescue administration.If the Ramsay score was still ≤ 4 points at 30 min after rescue administration, the examination was rescheduled or rescue drugs were given again by the anesthesiologists according to the condition and the urgency of the examination, with the number of administration not exceeding 3 times.The sedation failure was defined that the patients could not complete the diagnostic procedures after administration due to various reasons (including severe respiratory depression, rescheduled examination, parents' refusal to rescue administration, and Ramsay score still ≤ 4 points after 3 times of administration). The success of sedation at first attempt, success of rescue sedation and success of total sedation were recorded.The occurrence of sedation-related adverse events such as coughing, vomiting, allergies and respiratory depression was recorded.Compared with neonate group, the success rate of sedation at first attempt and success rate of total sedation were significantly increased, the incidence of sedation-related adverse events was decreased in infant, toddler and child groups ( P<0.01). There was no significant difference in the parameters mentioned above among infant group, toddler group and child group ( P>0.05). In conclusion, the efficacy of chloral hydrate for sedation is not good when used in neonates undergoing non-invasive diagnostic procedures.

15.
Article in Chinese | WPRIM | ID: wpr-932278

ABSTRACT

Objective:To study the influence of anticoagulation timing on incidence of perioperative deep venous thrombosis (DVT) in elderly patients with hip fracture.Methods:A retrospective analysis was made of the 179 elderly patients with hip fracture who had been admitted to Department of Orthopedics and Traumaology, Hong-Hui Hospital from July 2017 to December 2018. They were 78 males and 101 females, aged from 62 to 91 years (mean, 79.5 years). There were 79 femoral neck fractures and 100 intertrochanteric fractures, 109 of which were treated by internal fixation and 70 by hip replacement. The patients were divided into 3 groups depending on the timing of anticoagulation after injury. In group 1 of 74 cases, anticoagulation started <24 h after injury; in group 2 of 36 cases, anticoagulation started 24 to 48 h after injury; in group 3 of 69 cases, anticoagulation started >48 h after injury. Anticoagulation continued until 12 h before surgery in all patients but was resumed 8 to 12 h after surgery. The 3 groups were compared in incidence of perioperative DVT.Results:The 3 groups were comparable due to insignificant differences between them in their pre-operative general data ( P>0.05). DVT occurred perioperatively in 84 patients, yielding an incidence of 46.9% (84/179). The incidences of perioperative DVT were 27.0% (20/74), 47.2% (17/36) and 68.1% (47/69) in groups 1, 2 and 3, respectively, showing significant differences ( χ2=24.206, P<0.001), between any 2 groups ( P<0.05). Conclusion:Since the earlier anticoagulation starts after injury the lower incidence of perioperative DVT in elderly patients with hip fracture, early standardized prophylactic anticoagulation after injury can effectively reduce incidence of perioperative DVT.

16.
Article in Chinese | WPRIM | ID: wpr-865794

ABSTRACT

High-altitude physiology is a basic and core discipline of high-altitude medicine and also a required course for undergraduates majoring in high-altitude medicine. At present, high-altitude physiology teaching mainly adopts the traditional teaching mode, with a monotony teaching method. MOOC is a newly emerging online course model with advanced educational concepts. Under the background of the current military reform, the MOOC combined with the traditional teaching mode in high-altitude teaching can not only comprehensively improve the teaching quality and learning ability, but also effectively enhance the competency of students in high-altitude military medicine.

17.
Article in Chinese | WPRIM | ID: wpr-837628

ABSTRACT

Autophagy, a conserved intracellular degradation system, is a specific life phenomenon in eukaryocytes. Autophagy is widely accepted as a pathway that double-membrane autophagosomes envelop and sequester intracellular cytoplasmic components and then fuse with lysosomes to form autolysosomes, which degrade their contents to regenerate nutrients. Autophagy may be triggered by starvation and a diverse range of pathogens, including parasites. Following infection with intracellular parasites, host cells may eliminate parasites by autophagy. However, parasites may develop self-defense mechanisms, and promote the self-growth and -development by host cell autophagy. This review describes the advances in the interplay between parasitic infections and host cell autophagy. Understanding autophagy is of great significance for the management of parasitic infections and the development of antiparasitic drugs.

18.
Article in Chinese | WPRIM | ID: wpr-825236

ABSTRACT

Objective To analyze the advantages, disadvantages, opportunities and challenges for schistosomiasis elimination in Laos, so as to propose the corresponding healthy policies and suggestions. Methods A SWOT analysis was performed to analyze the strength, weakness, opportunity and threat for the schistosomiasis elimination program in Laos, and the corresponding policy suggestions were proposed. Results The national schistosomiasis elimination program of Laos receives governmental emphases and great supports. A strategy based on mass drug administration was proposed and a sentinel site-bases surveillance system has been built for schistosomiasis elimination in Laos; however, there are several challenges for the national schistosomiasis elimination program in Laos, including insufficient financial supports, inadequate professional capability, weak schistosomiasis control awareness in community populations and difficulty in vector control. Conclusions Persistent governmental leadership, increasing financial supports, strengthening professional team building and improving schistosomiasis control awareness in community populations are required to facilitate the progress towards schistosomiasis elimination in Laos.

19.
Article in Chinese | WPRIM | ID: wpr-802179

ABSTRACT

Objective:Fresh tubers of Gastrodiae Rhizoma were harvested at the right time. A saline water salting and drying technology was developed for obtaining the medicinal materials of Gastrodiae Rhizoma in the place of origin and avoiding rot and mildew. Method:Fresh tubers of Gastrodiae Rhizoma were dug in Yiliang,Yunnan,Dejiang,Guizhou,and Chenggu,Shaanxi,the experiments of natural drying,and saline water salting and drying were carried out in the place of origin and Beijing. After the dirt was removed,the samples were tiled in a container immediately,added with varied proportions of saline water (0.03-0.10 g·mL-1 NaCl in water),hermetically pickled for 6-15 d. after being soaked and rinsed with water,the samples were put in a cool ventilated place or under sunshine to prepare dried medicinal materials of Gastrodiae Rhizoma. We described the appearance characteristics,measured the moisture content,gastrodin and nitrite. And the appearance was observed after storage in a simple warehouse for one year later. Result:Fresh tubers of Gastrodiae Rhizoma from three origins were naturally dried,the surface of gastrodia tubers became black,decayed and moldy,then we could not get dried medicinal materials. The appearance and the content of gastrodins in the medicinal materials of Gastrodiae Rhizoma processed by saline water salting and drying technology met the requirements for Gastrodiae Rhizoma in the Pharmacopoeia of the People's Republic of China 2015 and relevant standards of nitrite in salted food in National Food Safety Standard Determination of Nitrite and Nitrate in Foods,Hygienic Standard for Preserved Vegetables,Green Food-Soybean Paste and Salted Vegetable. Conclusion:The saline water salting and drying technology is developed to make medicinal materials of Gastrodiae Rhizoma quickly from fresh tubers of Gastrodia elata in the place of origin and Beijing. The metamorphism had not been observed after being stored in simple warehouses for one year. This technology can guarantee the quality of Gastrodiae Rhizoma, and provide a new method for the filed processing of Gastrodiae Rhizoma.

20.
Article in Chinese | WPRIM | ID: wpr-801878

ABSTRACT

Objective:To explore the correlation between colors and contents of water and 5-hydroxymethylfurfural (5-HMF) in Ginseng Radix et Rhizoma Rubra (GRRR). Method:The colors were observed under sunlight on a sheet of white paper. The chroma value and color difference were determined and calculated by a colorimeter. Water content was determined by the oven-drying method. An HPLC method was established and used to determine the content of 5-HMF. The correlation was analyzed by the Spearman and Pearson analysis. Result:The colors of GRRR were yellowish-white,ocher-yellow,and yellowish-brown. The values of L*,a*,b*,and ΔE* had a certain range, and could reflect the colors of GRRR. The content of water ranged from 4.01%to 8.31%and was in accordance with the requirement the Chinese Pharmacopoeia. The content of 5-HMF ranged from below the limit of detection (1.985×10-4 μg) to 78.97 μg·g-1. The results of correlation analysis shows significant correlations between L*,a*,b*, ΔE* values and water content, that is to say,the deeper the color was, the higher the content of water was,but with no significant correlation with 5-HMF content. Conclusion:The color of processed GRRR was related to water content but not related to 5-HMF content,which was not consistent with the correlation between color and 5-HMF content that generally existed in a series of raw Chinese medicinal materials. It indicated an essential difference between the color shade of processed Chinese medicinal materials and the cause of color change of raw Chinese medicinal materials. Therefore,it was significant to control water content for ensuring the stability of GRRR. This study determined the colors of GRRR by a colorimeter, systematically determined 5-HMF content,and proposed the effect of water contents on the color of GRRR.

SELECTION OF CITATIONS
SEARCH DETAIL