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1.
Article in Chinese | WPRIM | ID: wpr-920643

ABSTRACT

@#With the development of medicinal and organic chemistry, more and more classical and non-classical bioisosteres are used in the design of novel drugs.Bicyclo[1.1.1] pentane (BCP), as a bioisostere of benzene, t-butyl and alkyne moieties,has recently received extensive attention from medicinal and organic chemists.This paper briefly reviews its application in drug design to provide reference for drug discovery researchers.

2.
Journal of Preventive Medicine ; (12): 129-134, 2022.
Article in Chinese | WPRIM | ID: wpr-920565

ABSTRACT

Objective@#To examine the effects of obesity and central obesity on hypertension, so as to provide insights into the prevention and control measures of hypertension.@*Methods@#From September to December 2018, residents at ages of 35 to 75 years were sampled using the multi-stage random sampling method in Baiyin District, Baiyin City, Gansu Province, and subjected to questionnaire surveys and physical examinations. The interaction between obesity/central obesity and hypertension was evaluated using logistic regression analysis. The synergy index ( SI ), relative excess risk due to interaction ( RERI ) and attributable proportion due to interaction ( AP ) were calculated using Excel compiled by Andersson et al.@*Results@#A total of 6 246 questionnaires were allocated and 6 169 valid questionnaires were recovered, with an effective recovery rate of 98.77%. The respondents included 3 038 men ( 49.25% ) and 3 131 women (50.75%), with a mean age of ( 52.05±8.78 ) years. There were 832 respondents with obesity ( 13.49% ) and 2 278 with central obesity ( 36.93% ). The crude and standardized prevalence rates of hypertension were 35.89% and 33.05%, respectively. Multivariable logistic regression analysis showed that obesity ( OR=2.020, 95%CI: 1.705-2.393 ) and central obesity ( OR=1.622, 95%CI: 1.433-1.836 ) were statistically associated with hypertension. There was no multiplicative interaction between obesity or central obesity and hypertension ( OR=1.011, 95%CI: 0.655-1.560 ), and no additive interaction was detected between obesity or central obesityand hypertension ( SI=1.405, 95%CI: 0.815-2.424; RERI=0.658, 95%CI: -0.298 to 1.614; AP=0.201, 95%CI: -0.075 to 0.476 ).@*Conclusions@#Obesity and central obesity increase the risk of hypertension; however, no interaction is detected between obesity or central obesity and hypertension.

3.
Journal of Leukemia & Lymphoma ; (12): 652-657, 2021.
Article in Chinese | WPRIM | ID: wpr-907230

ABSTRACT

Objective:To investigate the clinical efficacy of autologous peripheral blood hematopoietic stem cell transplantation (HSCT) in treatment of lymphoma.Methods:The clinical data of 41 lymphoma patients undergoing autologous peripheral blood HSCT at the Affiliated Hospital of Jining Medical University between January 2014 to December 2020 were retrospectively analyzed. There were 6 cases of Hodgkin lymphoma and 35 cases of non-Hodgkin lymphoma. The mobilization regimens included chemotherapy drugs + granulocyte colony-stimulating factor (G-CSF) + thrombopoietin (TPO) or chemotherapy drugs + G-CSF. The pre-conditioning schemes before transplantation were listed as follows: BEAM (mustine + cytarabine + etoposide + melphalan) regimen + decitabine in 26 patients, BEAM regimen in 12 patients, BEAM regimen + chidamide in 3 patients. The progression-free survival (PFS), overall survival (OS), related complications, prognoses after transplantation were observed. The effects of clinical staging, B symptom,International Prognostic Score Index (IPI), extranodal involved sites, hemoglobin (Hb), lactic dehydrogenase (LDH), β 2-microglobulin (β 2-MG), transplantation regimen and the status before transplantation on PFS and OS after transplantation were evaluated. Results:Among 41 patients, 37 patients (90.24%) achieved complete remission (CR), 2 patients (4.88%) achieved partial remission (PR) and 2 patients loss assessment data (4.88%) before autologous peripheral blood HSCT. The median karyocyte count was 12.74×10 8 /kg [(3.91-22.68)×10 8/kg] in 24 patients with the complete data of stem cell collection, the median CD34 positive cell count was 6.74×10 6/kg [(0.91-50.47)×10 6/kg]. All 41 patients had hematologic reconstruction. The median time of platelet implantation was 11 d (7-32 d) and the median time of granulocyte implantation was 9 d (8-16 d). All patients achieved CR after transplantation and no one case had transplantation-related death. By the end of follow-up, 33 cases (80.49%) had no progression of disease, 8 cases (19.51%) died. The OS rates of 12-month, 24-month and 72-month were 93.4%, 85.3% and 60.9%, respectively after transplantation. The PFS rates of 12-month, 24-month and 72 month were 93.3%, 84.0% and 84.0%, respectively. Median PFS and OS had not been reached. There were no statistically significant differences in the PFS and OS of patients with different gender, clinical staging, B symptom, IPI score, extranodal involved sites, Hb, LDH, β 2-MG and the status before transplantation(all P > 0.05) . The PFS and OS of patients receiving BEAM regimen + decitabine were better than those of patients receiving BEAM regimen alone (all P < 0.05). Conclusions:Autologous peripheral blood HSCT is effective in treatment of lymphoma. Moreover, BEAM regimen + dicitabine preconditioning regimen can achieve longer survival time compared with BEAM regimen alone.

4.
Article in Chinese | WPRIM | ID: wpr-905853

ABSTRACT

Adequate sleep is an important factor to ensure the healthy functioning of the body. A type of chronic sleep diseases characterized by insufficient sleep can be collectively referred to as sleep deprivation (SD), which is divided into primary and secondary sources in terms of sources. As one of the most frequent types of diseases in recent years, SD has received more and more attention and attention from the whole society. SD can have a wide-ranging and far-reaching impact on cognitive behavior, such as decreased wakefulness, decreased alertness, and inattention, decreased sensory perception, decreased learning and memory capabilities, et al, involving the impact on multiple system functions of the human body, and It is closely related to the occurrence of many diseases, and may cause serious troubles to the normal life of patients and even their relatives and friends. The cognitive impairment caused by SD has been fully verified in clinical tests and various animal behavior experiments, mainly involving pathological damage such as changes in synaptic plasticity, enhanced endoplasmic reticulum stress, circadian rhythm disorders, and energy metabolism imbalance. Western medicine treatments for SD mostly have negative factors such as high side effect and strong addiction. However, Chinese medicine intervention focuses on the overall concept, has long-lasting effectiveness, significant effects, and mild side effects. It has also been widely recognized clinically for improving the complications of sleep disorders. This article reviews the current status and classification of SD research, its pathological mechanisms that lead to cognitive impairment and its molecular-level exploration directions and results. In recent 5 years, the therapeutic effect and experience of traditional Chinese medicine intervention therapy such as compound Chinese medicine, acupuncture and moxibustion as well as auxiliary therapy such as exercise and five sounds, in order to further summarize and clarify the interaction mechanism between SD and cognitive behavior, and provide a theoretical basis for the study of the pathological mechanism of SD disease and future clinical treatment.

5.
Article in Chinese | WPRIM | ID: wpr-905072

ABSTRACT

Objective:to explore the mechanism of modified Tianwang Buxindan in improving abnormal glucose and lipid metabolism in mice with chronic sleep deprivation from the signal pathway of orexin A/ orexin receptor 1(OX1R). Method:The 50 6-week-old male C57BL/6 mice were randomly divided into blank group , model group , estazolam group and Tianwang Buxindan low and high dose groups ,for ten mice of each group. Except the blank group, rats were deprived of sleep for 8 weeks by the method of multi-platform water environment. In the last 4 weeks, Tianwang Buxindan (8.5,17 g·kg-1)and estazolam solution(9.1 mg·kg-1)were given to the stomach, and the blank group and the model group were fed with pure water of the same volume. The food intake and body weight of mice were measured twice a week, on the 49th day, blood samples were collected from the tail vein for glucose tolerance test (GTT),on the 52nd day for insulin tolerance test(ITT), was used to detect the expression of total cholesterol (TCH), triglyceride(TG)and free fatty acid(FFA)in serum, and enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of orexin A in serum and hypothalamus. Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR)and Western blot were used to detect the mRNA and protein expression of OX1R in hypothalamus. Result:After administration, the food intake of mice in each group was different, compared with the blank group, the body weight of model group was significantly reduced(P<0.05), the glucose tolerance was significantly abnormal, and the TCH, TG, FFA values were significantly increased(P<0.01). The expression of orexin A in serum and hypothalamus increased significantly(P<0.01), and the mRNA and protein expression levels of OX1R in hypothalamus increased significantly(P<0.01). Compared with the model group, the body weight of each group of Tianwang Buxindan was significantly increased(P<0.05), with better glucose tolerance and insulin sensitivity, TCH, TG, FFA values were significantly reduced(P<0.05,P<0.01), accompanied by serum and the expression of orexin A in the hypothalamus was significantly decreased(P<0.05,P<0.01), the mRNA and protein expression levels of OX1R were significantly decreased(P<0.05,P<0.01). Conclusion:Tianwang Buxindan can protect mice from abnormal glucose and lipid metabolism induced by chronic sleep deprivation, and its mechanism may be related to the down-regulation of orexin A/OX1R signal expression.

6.
China Pharmacy ; (12): 2672-2676, 2021.
Article in Chinese | WPRIM | ID: wpr-904529

ABSTRACT

OBJECTIVE:To introduce the role of clinical pharmacists in the treatment of camrelizumab-induced toxic epidermal necrolysis (TEN),and to provide reference for the therapy of similar ADR. METHODS:The clinical pharmacist participated in therapy duration of a patient with TEN caused by camrelizumab. The patient was treated with Camrelizumab for injection combined with Apatinib mesylate tablet as anti-tumor therapy,and was admitted to hospital due to extensive skin lesions. After consulting relevant literatures and analyzing the patient’s admission diagnosis [severe epidermolysis bullosa,severe drug eruption(erythema multiforme),abnormal liver function,etc.] and examination results(hypokalemia,etc.),clinical pharmacists suggested to stop above anti-tumor drugs and given Methylprednisolone sodium succinate for injection(160 mg→80 mg→60 mg, qd,ivgtt) for anti-inflammatory treatment,Imiperan cilastatin for injection (1 g,q8 h,ivgtt) for an ti-infection treatment, Potassium chloride injection(1 g,qd,ivgtt)for electrolyte regulation,Compound amino acid injection(3AA)(10.65 g,qd,ivgtt) for nutritional support treatment,Pantoprazole sodium for injection(40 mg,qd,ivgtt)for acid inhibition and stomach protection, Reduced glutathione for injection(2.4 g,qd,ivgtt)for liver protection. Before medication,the patient was given cognitive and behavioral education and medication publicity. The changes of relevant indicators were closely monitored during medication,and the patient was given medication guidance when he was discharged. RESULTS:The doctors adopted the suggestions of clinical pharmacists,and 16 days after treatment,the skin lesions of the trunk and limbs were improved,and the double eyelids were still erosive and exuded a lot of secretions. The patient requested transfer for treatment. CONCLUSIONS:Clinical pharmacists assisted physicians to improve the treatment plan of patients with TEN caused by camrelizumab,and carried out cognitive and behavioral education and medication publicity for patients to ensure the effectiveness and safety of their medication.

7.
Article in Chinese | WPRIM | ID: wpr-879009

ABSTRACT

Phylogeography is a research hotspot in the field of the genetic diversity and core germplasm construction of endangered rare plants. Paris polyphylla var. yunnanensis is a rare plant species mainly distributed in China. Wild individuals have been overexploited for the last few decades because of increasing demand for such medicines. Therefore, it is great significance to study the phylogeography of P. poliphylla var. yunnanensis based on chloroplast gene trnL-trnF sequences. In this study, chloroplast genes trnL-trnF were used in the phylogeography analysis of 15 wild and 17 cultivated populations of P. polyphylla var. yunnanensis. This study revealed that based on the results of neutrality tests and mismatch analysis, the rapid expansion of wild population has not been detected in P. polyphylla var. yunnanensis. After aligning and sorting the obtained cpDNA sequences, a total of 15 haplotypes were detected in all 32 populations. One haplotype was unique to the wild population, and 5 haplotypes were unique to the cultivated population. It can be seen that the haplotype richness of cultivated population was higher than that of wild population. The wild populations of P. polyphylla var. yunnanensis were divided into two groups according to evolutionary relationship of haplotypes and distribution map of haplotypes. The haplotype of branch Ⅰ was mainly distributed in Guizhou, and the haplotype of branch Ⅱ was located in Yunnan and Huidong, Sichuan. Therefore, it's speculated that Guizhou and the west Yunnan region may be glacial refuge in the evolutionary history of wild populations of P. polyphylla var. yunnanensis, and in order to protect the wild resources more effectively, wild populations of P. polyphylla var. yunnanensis in these two areas should be included in the protection zone.


Subject(s)
China , Genes, Chloroplast , Humans , Liliaceae/genetics , Melanthiaceae , Phylogeography
8.
Article in Chinese | WPRIM | ID: wpr-881236

ABSTRACT

@#Objective    To study the learning curve of minimal invasive coronary artery bypass grafting (MICS CABG) and the influence on the perioperative clinical effects by analyzing operation time. Methods    From March 2012 to November 2020, 212 patients underwent MICS CABG by the same surgeon. Among them, 59 patients (52 males and average age of 62.89±8.27 years) with single vessel bypass grafting were as a single-vessel group and 153 patients (138 males, average age of 59.80±9.22 years) with multi-vessel bypass grafting were as a multi-vessel group. Two sets of operation time-operation sequence scatter plots were made and learning curve was analyzed by cumulative summation (CUSUM) and regression method of operation time. The surgical data of each group before and after the inflection point of the learning curve were compared with the main clinical outcome events within 30 days after surgery. Results    There was no death, perioperative myocardial infarction and stroke in 212 MICS CABG patients and no transfer to cardiopulmonary bypass or redo thoracotomy. The learning curve conformed to the cubic fitting formula. In the single- vessel group, CUSUM (x operation number)=–1.93+93.45×x–2.33×x2+0.01×x3, P=0.000, R2=0.986, the tipping point was 27 patients. In the multi-vessel group, CUSUM (x)=y=2.87+1.15×x–1.29× x2+3.463×x3, P=0.000, R2=0.993, and the tipping point was 59 patients. The two sets of case data were compared before and after the learning curve and there was no statistical difference in main clinical outcomes within 30 days (mortality, acute myocardial infarction, stroke, perioperative blood transfusion rate), ventilator tube, and intensive care unit retention. Conclusion    The learning curve of MICS CABG conforms to the cubic formula, and the process transitions from single to multiple vessels bypass. To enter the mature stage of the learning phase, a certain number of patients need to be done. Reasonable surgical procedures and quality control measures can ensure the safety during the learning phase.

9.
Journal of Experimental Hematology ; (6): 1050-1055, 2021.
Article in Chinese | WPRIM | ID: wpr-888517

ABSTRACT

OBJECTIVE@#To investigate the effect of sulforaphane (SFN) on G@*METHODS@#KG1a and KG1cells were treated by different concentrations of SFN for 48 h. Flow cytometry (FCM) was used to analyze the phase distribution of cell cycle. High-throughput sequencing was used to detect the effect of SFN on the expression of cell cycle related genes in KG1a cells. The mRNA expression of P53, P21, CDC2 and CyclinB1 were detected by qPCR. The protein expression of P53, CDC2, P-CDC2 and CyclinB1 were detected by Western blot.@*RESULTS@#Cells in the G@*CONCLUSION@#SFN induces leukemia cells to block in G


Subject(s)
Cell Cycle , Humans , Isothiocyanates/pharmacology , Leukemia, Myeloid, Acute , Mitosis , Sulfoxides
10.
Article in Chinese | WPRIM | ID: wpr-883025

ABSTRACT

Objective:To explore the effect of diversified nursing rehabilitation exercise in patients with SchatzkerⅡtibial plateau fracture.Methods:From January 2018 to December 2018, a total of 72 patients with Schatzker Ⅱtibial plateau fractures in Wuzhou traditional Chinese medicine hospital were selected by cluster sampling method for study. All patients were treated surgically. 36 of them were included in the control group (routine functional exercise after operation) by random number table method, and the remaining 36 patients were included in the observation group (on the basis of the control group, multiple functional rehabilitation exercise mode was given).The length of hospital stay, knee joint swelling degree and movement angle at different time after operation, preoperative and postoperative Harris Hip Score (HSS) and satisfaction were analyzed.Results:The time of fracture healing and hospitalization in the observation group were (92.58±20.65) d, (8.40±2.32) d, shorter than (108.56±25.38) d, (15.86±2.80) d in the control group ( tvalues were 2.930, 12.309, P<0.05). At 2 weeks and 6 weeks after operation, the swelling degree of knee joint in the observation group was (1.42±0.58) cm and (0.52±0.25) cm, which was less than (1.86±0.62) cm and (0.78±0.34) cm of the control group, respectively, the difference between the two groups was statistically significant ( tvalues were 3.110, 3.697, P<0.05); there was no significant difference in HSS between the two groups ( P>0.05); the score of observation group was (62.36±6.85), (70.32±5.69) points, which was significantly higher than (58.42±6.02), (63.45±6.28) points of control group ( tvalues were 2.592, 4.864, P<0.05); there was no significant difference in HSS score between the two groups 6 months after operation ( P>0.05). The satisfaction of the observation group was 97.22% (35/36), which was higher than 83.33% (30/36) of the control group, and the difference between the two groups was statistically significant ( χ 2value was 3.956, P<0.05). Conclusion:For Schatzker type Ⅱ tibial plateau fracture patients, taking diversified nursing rehabilitation exercise can quickly relieve their limb swelling, promote the recovery of joint function and fracture healing, make them recover as soon as possible, and improve their satisfaction.

11.
Article in English | WPRIM | ID: wpr-897327

ABSTRACT

Thyroid cancer is the most common endocrine malignancy. Patients with well-differentiated thyroid cancers, such as papillary and follicular cancers, have a favorable prognosis. However, poorly differentiated thyroid cancers, such as medullary, squamous and anaplastic advanced thyroid cancers, are very aggressive and insensitive to radioiodine treatment. Thus, novel therapies that attenuate metastasis are urgently needed. We found that both PDGFC and PDGFRA are predominantly expressed in thyroid cancers and that the survival rate is significantly lower in patients with high PDGFRA expression. This finding indicates the important role of PDGF/PDGFR signaling in thyroid cancer development. Next, we established a SW579 squamous thyroid cancer cell line with 95.6% PDGFRA gene insertion and deletions (indels) through CRISPR/Cas9. Protein and invasion analysis showed a dramatic loss in EMT marker expression and metastatic ability. Furthermore, xenograft tumors derived from PDGFRA geneedited SW579 cells exhibited a minor decrease in tumor growth. However, distant lung metastasis was completely abolished upon PDGFRA gene editing, implying that PDGFRA could be an effective target to inhibit distant metastasis in advanced thyroid cancers. To translate this finding to the clinic, we used the most relevant multikinase inhibitor, imatinib, to inhibit PDGFRA signaling. The results showed that imatinib significantly suppressed cell growth, induced cell cycle arrest and cell death in SW579 cells. Our developed noninvasive apoptosis detection sensor (NIADS) indicated that imatinib induced cell apoptosis through caspase-3 activation. In conclusion, we believe that developing a specific and selective targeted therapy for PDGFRA would effectively suppress PDGFRA-mediated cancer aggressiveness in advanced thyroid cancers.

12.
Article in English | WPRIM | ID: wpr-889623

ABSTRACT

Thyroid cancer is the most common endocrine malignancy. Patients with well-differentiated thyroid cancers, such as papillary and follicular cancers, have a favorable prognosis. However, poorly differentiated thyroid cancers, such as medullary, squamous and anaplastic advanced thyroid cancers, are very aggressive and insensitive to radioiodine treatment. Thus, novel therapies that attenuate metastasis are urgently needed. We found that both PDGFC and PDGFRA are predominantly expressed in thyroid cancers and that the survival rate is significantly lower in patients with high PDGFRA expression. This finding indicates the important role of PDGF/PDGFR signaling in thyroid cancer development. Next, we established a SW579 squamous thyroid cancer cell line with 95.6% PDGFRA gene insertion and deletions (indels) through CRISPR/Cas9. Protein and invasion analysis showed a dramatic loss in EMT marker expression and metastatic ability. Furthermore, xenograft tumors derived from PDGFRA geneedited SW579 cells exhibited a minor decrease in tumor growth. However, distant lung metastasis was completely abolished upon PDGFRA gene editing, implying that PDGFRA could be an effective target to inhibit distant metastasis in advanced thyroid cancers. To translate this finding to the clinic, we used the most relevant multikinase inhibitor, imatinib, to inhibit PDGFRA signaling. The results showed that imatinib significantly suppressed cell growth, induced cell cycle arrest and cell death in SW579 cells. Our developed noninvasive apoptosis detection sensor (NIADS) indicated that imatinib induced cell apoptosis through caspase-3 activation. In conclusion, we believe that developing a specific and selective targeted therapy for PDGFRA would effectively suppress PDGFRA-mediated cancer aggressiveness in advanced thyroid cancers.

13.
Journal of Experimental Hematology ; (6): 1617-1622, 2021.
Article in Chinese | WPRIM | ID: wpr-922305

ABSTRACT

OBJECTIVE@#To establish a mouse model of respiratory syncytial virus (RSV) infection after hematopoietic stem cell transplantation, so as to lay the foundation for future research on RSV infection and related complications after hematopoietic stem cell transplantation.@*METHODS@#Bone marrow cells and spleen cells were transplanted to C57BL/6 mice after myeloablative treatment to establish a mouse model of allogeneic hematopoietic stem cell transplantation. The chimerism rate was detected by flow cytometry 3 and 7 weeks after transplantation. The transplanted mice were infected with RSV by nasal drops. The lung tissues were collected 5 days after infection for identification of infection, and lung tissues were analyzed for pathology 2 weeks and 2 months after infection.@*RESULTS@#The chimerism rate was > 90% at 3 and 7 weeks after transplantation. Successful infection was detected 5 days after RSV infection, and there were severe and persistent pathological changes in the lung tissues of the mice 2 weeks and 2 months after infection.@*CONCLUSION@#RSV infection in stable chimeric mice after hematopoietic stem cell transplantation can cause significantly persistent lung disease, which lays foundation for the prevention and treatment of RSV infection and the mechanism of later bronchiolitis obliterans after hematopoietic stem cell transplantation.


Subject(s)
Animals , Chimerism , Disease Models, Animal , Hematopoietic Stem Cell Transplantation , Mice , Mice, Inbred C57BL , Respiratory Syncytial Virus, Human
14.
Chinese Medical Journal ; (24): 1450-1456, 2021.
Article in English | WPRIM | ID: wpr-878191

ABSTRACT

BACKGROUND@#Drug-coated balloons (DCBs) have emerged as potential alternatives to drug-eluting stents in specific lesion subsets for de novo coronary lesions. Quantitative flow ratio (QFR) is a method based on the three-dimensional quantitative coronary angiography and contrast flow velocity during coronary angiography (CAG), obviating the need for an invasive fractional flow reserve procedural. This study aimed to assess the serial angiographic changes of de novo lesions post-DCB therapy and further explore the cut-off values of lesion and vessel QFR, which predict vessel restenosis (diameter stenosis [DS] ≥50%) at mid-term follow-up.@*METHODS@#The data of patients who underwent DCB therapy between January 2014 and December 2019 from the multicenter hospital were retrospectively collected for QFR analysis. From their QFR performances, which were analyzed by CAG images at follow-up, we divided them into two groups: group A, showing target vessel DS ≥50%, and group B, showing target vessel DS <50%. The median follow-up time was 287 days in group A and 227 days in group B. We compared the clinical characteristics, parameters during DCB therapy, and QFR performances, which were analyzed by CAG images between the two groups, in need to explore the cut-off value of lesion/vessel QFR which can predict vessel restenosis. Student's t test was used for the comparison of normally distributed continuous data, Mann-Whitney U test for the comparison of non-normally distributed continuous data, and receiver operating characteristic (ROC) curves for the evaluation of QFR performance which can predict vessel restenosis (DS ≥50%) at mid-term follow-up using the area under the curve (AUC).@*RESULTS@#A total of 112 patients with 112 target vessels were enrolled in this study. Group A had 41 patients, while group B had 71. Vessel QFR and lesion QFR were lower in group A than in group B post-DCB therapy, and the cut-off values of lesion QFR and vessel QFR in the ROC analysis to predict target vessel DS ≥50% post-DCB therapy were 0.905 (AUC, 0.741 [95% confidence interval, CI: 0.645, 0.837]; sensitivity, 0.817; specificity, 0.561; P < 0.001) and 0.890 (AUC, 0.796 [95% CI: 0.709, 0.882]; sensitivity, 0.746; specificity, 0.780; P < 0.001).@*CONCLUSIONS@#The cut-off values of lesion QFR and vessel QFR can assist in predicting the angiographic changes post-DCB therapy. When lesion/vessel QFR values are <0.905/0.890 post-DCB therapy, a higher risk of vessel restenosis is potentially predicted at follow-up.


Subject(s)
Constriction, Pathologic , Coronary Angiography , Coronary Artery Disease/therapy , Coronary Restenosis , Follow-Up Studies , Fractional Flow Reserve, Myocardial , Humans , Pharmaceutical Preparations , Predictive Value of Tests , Retrospective Studies , Treatment Outcome
15.
International Eye Science ; (12): 847-853, 2021.
Article in Chinese | WPRIM | ID: wpr-876012

ABSTRACT

@#AIM: To systematically evaluate the efficacy of sodium hyaluronate combined with recombinant human epidermal growth factor(rhEGF)eye drops in the treatment of dry eye after cataract surgery in recent 5a by Meta-analysis.<p>METHODS: The databases of CNKI, WanFang, Chongqing VIP, CBM, Cochrane Library, Pudmed and MEDLINE from January 2015 to May 2020 were searched. Meta analysis and statistical analysis were performed with Revman 5.3 software on the clinical control study of sodium hyaluronate eye drops combined with rhEGF eye drops in the treatment of dry eye after cataract surgery.<p>RESULTS: A total of 14 randomized controlled trials involving 1 529 eyes were included. Among them, 14 studies conducted corneal fluorescein staining(FL)score after the end of treatment, the results showed that the difference was statistically significant \〖<i>MD</i>= -0.86, 95%<i>CI</i>(-1.07 to -0.66), <i>P</i><0.00001\〗; 14 studies conducted tear film break-up time(BUT)measurement after the end of treatment, the results showed that the difference was statistically significant \〖<i>MD</i>=2.33, 95%<i>CI</i>(1.64, 3.03), <i>P</i><0.00001\〗, 95%<i>CI</i>(1.76 to 2.15), <i>P</i><0.00001\〗; tear secretion test(S I t)was measured in 12 studies after treatment, and the results showed that there was no significant difference \〖<i>MD</i>=0.49, 95%<i>Cl</i>(-0.52, 1.50), <i>P</i>=0.34\〗; the overall effective rate was calculated in 12 studies after treatment, and the results showed that the difference was statistically significant \〖<i>OR</i>=4.88, 95%<i>Cl</i>(3.34, 1.50), <i>P</i>=0.34\〗. The results showed that the difference was statistically significant \〖<i>MD</i>= -1.09, 95%<i>Cl</i>(-1.58, -0.61), <i>P</i><0.00001\〗.<p>CONCLUSION: Sodium hyaluronate combined with rhEGF eye drops in the treatment of dry eye after cataract surgery can significantly improve the corneal repair ability and tear film BUT, which has more advantages in the overall efficacy of the treatment of dry eye after cataract surgery.

16.
Acta Pharmaceutica Sinica ; (12): 520-527, 2021.
Article in Chinese | WPRIM | ID: wpr-873785

ABSTRACT

We investigated the inhibitory effect and mechanism of action of bruceantin (BCT) on the proliferation, invasion and migration of non-small cell lung cancer (NSCLC) cells. The cytotoxic activity of BCT was measured by MTT assay; a colony forming assay, wound healing assay, and a Transwell assay were used to investigate the anti-proliferative, anti-migration, and anti-invasion effects, respectively; immunoblotting and RT-qPCR were used to detect the expression of related proteins, miRNA, and mRNA, respectively, that were involved in cell proliferation, migration, and invasion. Two gene prediction websites were used to predict the downstream target gene of miRNA. Our results show that BCT has a potent cytotoxic effect on NSCLC cell lines, with a half maximal inhibitory concentration (IC50) of BCT against H1299, PC-9, and A549 of 0.12 ± 0.02, 0.31 ± 0.20, and 2.07 ± 0.70 μmol·L-1, respectively. When H1299 cells were treated with 0.03, 0.15, and 0.75 μmol·L-1 BCT for 24 h, the proliferation, migration, and invasive ability were inhibited in a concentration-dependent manner. It is worth noting that the expression level of miRNAs related to cell migration and invasion, such as miR-29a-3p, miR-21-3p, miR-183-5p, and miR-34b-5p increased with the concentration of BCT, especially for miR-29a-3p. Using the two gene prediction websites, we predict that integrin β1 (ITGB1) may be the target gene of miR-29a-3p; immunoblot results further show that a variety of proteins related to cell proliferation, migration, and invasion, such as various proteins of the integrin family, β-catenin, p-Src, and vascular endothelial growth factor, all decreased in a concentration-dependent manner, among which the reduction of ITGB1 protein was the most obvious. RT-qPCR results showed that there was no change in ITGB1 mRNA expression. We speculate that BCT might inhibit the expression of ITGB1 protein by up-regulating miR-29a-3p independent of its mRNA level. The in-depth mechanism needs to be further explored. This study suggests that BCT has the potential for further development in the treatment of NSCLC.

17.
Article in Chinese | WPRIM | ID: wpr-873619

ABSTRACT

@#Objective    To investigate the safety and effectiveness of minimally invasive coronary artery bypass grafting (MICABG) through comparing the perioperative clinical effects of conventional surgery and MICABG. Methods    A total of 543 patients in the single medical group of Beijing Anzhen Hospital who underwent beating coronary artery bypass grafting from January 2017 to September 2020 were collected, including 161 patients receiving MICABG (a minimally invasive group, 143 males and 18 females, aged 60.08±9.21 years), 382 patients receiving median thoracotomy (a conventional group, 284 males and 98 females, aged 61.68±8.81 years). The propensity score was used to match 143 patients in each of the two groups, and the perioperative data of the two groups were summarized and analyzed. Results    There was no death, perioperative myocardial infarction or stroke in the minimally invasive group. Compared with the conventional group, the minimally invasive group had longer operation time (296.36±89.4 min vs. 217.80±50.63 min, P=0.000), less number of bypass grafts (2.86±1.03 vs. 3.17±0.78, P=0.005), shorter postoperative hospital stay (6.29±1.46 d vs. 6.78±2.61 d, P=0.031), less drainage on postoperative day 1 (339.57±180.63 mL vs. 441.92±262.63 mL, P=0.001) and lower usage rate of inotropic drugs (9.09% vs. 26.57%, P=0.001). There was no statistical difference between the two groups in postoperative ICU stay ventilator assistance time, blood transfusion rate, secondary thoracotomy rate, or use of mechanical equipment. Conclusion    Reasonable clinical strategies can ensure the satisfactory overall safety of MICABG. In addition, it has the advantages of shorter postoperative hospital stay, less bleeding and smaller dosage of inotropic drugs.

18.
Article in English | WPRIM | ID: wpr-888655

ABSTRACT

OBJECTIVE@#To investigate the protective effects of Shexiang Tongxin Dropping Pill (, STDP) following sodium laurate-induced coronary microembolization (CME) in rats.@*METHODS@#Forty rats were divided into 4 groups: the control (sham) group, CME group, low-dose STDP pretreatment group (20 mg·kg@*RESULTS@#The rats in the CME group showed a significant increase in the fibrinogen-like protein 2 expression level and mitochondrial dysfunction and a decrease in the expression level of antioxidant biomarkers (superoxide dismutase and catalase, P<0.01 for all). In contrast, the rats in the low- and high-dose STDP pretreatment groups showed a significant decrease in coronary microthrombi (P<0.05); moreover, STDP restored the antioxidant-related protein activities and mitochondrial function, inhibited mPTP opening, decreased AKT-Ser473 phosphorylation, and increased GSK3β-Ser9 phosphorylation (P<0.05 or P<0.01).@*CONCLUSION@#STDP may be useful for treatment of CME, possibly via regulation of mPTP opening and AKT/GSK3β phosphorylation.

19.
J Cancer Res Ther ; 2020 Sep; 16(5): 1014-1019
Article | IMSEAR | ID: sea-213748

ABSTRACT

Context: It is necessary to explore a minimally invasive, effective, and efficient treatment for those lung cancer patients who are poor candidates for surgery. Aim: This study aimed to investigate the application of microwave ablation (MWA) in the treatment of lung cancer. Settings and Design: A total of 43 patients with 44 pulmonary lesions were examined following identical procedures before being randomly divided into two groups. The experimental group consists of 17 patients with a total of 18 pulmonary lesions, while the control group consists of 26 patients with a total of 26 pulmonary lesions. Materials and Methods: The experimental group was treated using magnetic resonance imaging (MRI)-guided MWA while the control group was treated using computer tomography (CT)-guided MWA. A transverse relaxation time-turbo spin echo (T2-TSE) sequence was used for signal collection in the experimental group to determine puncture location and microwave needle position while T2-TSE, T1-turbo field echo, and diffusion-weighted MRI (DWI) sequences were used for timely efficacy evaluation. Whereas in the control group, CT axial scanning was performed to serve similar purposes. Statistical Analysis Used: A nonparametric Wilcoxon test, median (M [25%, 75%]). Results: All of the 44 lesions were successfully located on the first attempt. The mean time for scanning and locating lung lesions under MRI and CT guidance were 64.53 and 42.96 min, the mean times of positioning were 12 and 18 min, and the mean durations of MWA were 12.48 and 15.06 min, respectively. Conclusions: As a minimally invasive method for treating lung tumors, MRI-guided MWA requires fewer localization scans, a shorter MWA duration, no radiation, real-time observation of the curative effect, and it prevents overtreatment

20.
China Pharmacy ; (12): 217-220, 2020.
Article in Chinese | WPRIM | ID: wpr-817365

ABSTRACT

OBJECTIVE:To provide reference for safe and rational use of Sodium tanshinone ⅡA sulfonate(STS)injection in the clinic. METHODS :The information of the patients who received STS injection from Jan. 2016 to Dec. 2017 were collected from a Grade 3 hospital. According to relevant suggestions in drug package inserts ,drug utilization rationality was evaluated ,and single-factor and multi-factor analysis on the risk and influential factors for ADR/ADE were performed by group design and individual matching to examine their correlation. RESULTS :Totally 3 283 patients were included in the study. The drug use frequency were less than 1.5,and the drug utilization indexes were less than 1.0,suggesting that the hospital using STS injection was basically reasonable. Irrational use of drugs mainly included that inappropriate indications (46.48%),unreasonable solvent selection(15.84%),and excessive concentration (2.71%). Patients with renal insufficiency received STS injection ,and then the risk of ADR/ADE increased by correlation analysis (P<0.05). CONCLUSIONS :Irrational use of STS injection in clinics existed , mainly like off-label drug use ,excessive concentration ,irrational solvent selection. Drug use evaluation and monitoring should be strengthened. For patients with renal insufficiency ,it is necessary to prevent the occurrence of ADR/ADE .

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