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Article in English | WPRIM | ID: wpr-285262


The association between high-density lipoprotein cholesterol (HDL-C) and mortality in patients with acute aortic dissection (AAD) is unclear. From January 2007 to January 2014, a total of 928 consecutive AAD patients who were admitted within 48 h after the onset of symptoms were enrolled in the study. Patients were divided into two groups according to whether serum HDL-C level was below the normal lower limit or not. The Cox proportional hazard regression model was used to identify the predictive value of HDL-C for in-hospital mortality in patients with AAD. As compared with normal HDL-C group (n=585), low HDL-C group (n=343) had lower levels of systolic blood pressure and hemoglobin and higher levels of leukocyte, alanine aminotransferase, blood glucose, blood urea nitrogen, creatinine and urea acid. Low HDL-C group had significantly higher in-hospital mortality than normal HDL-C group (21.6% vs. 12.6%, log-rank=10.869, P=0.001). After adjustment for baseline variables including demographics and biologic data, the increased risk of in-hospital mortality in low HDL-C group was substantially attenuated and showed no significant difference (adjusted hazard ratio, 1.23; 95% confidence interval, 0.86-1.77; P=0.259). Low HDL-C is strongly but not independently associated with in-hospital mortality in patients with AAD.

Acute Disease , Adult , Aged , Alanine Transaminase , Blood , Aneurysm, Dissecting , Blood , Diagnosis , Mortality , Pathology , Aortic Aneurysm , Blood , Diagnosis , Mortality , Pathology , Biomarkers , Blood , Blood Glucose , Metabolism , Blood Pressure , Blood Urea Nitrogen , Cholesterol, HDL , Blood , Cholesterol, LDL , Blood , Creatinine , Blood , Female , Hospital Mortality , Humans , Male , Middle Aged , Proportional Hazards Models , Risk Factors , Uric Acid , Blood
Article in English | WPRIM | ID: wpr-250365


The aim of the present study is to investigate how cytochrome P450 enzymes (CYP) 2C8-derived epoxyeicosatrienoic acids (EETs) regulate the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway and protect against oxidative stress-induced endothelial injuries in the development and progression of atherosclerosis. In this study, cultured human umbilical vein endothelial cells (HUVECs) were transfected with CYP2C8 or pretreated with exogenous EETs (1 μmol/L) before TNF-α (20 ng/mL) stimulation. Apoptosis and intracellular ROS production were determined by flow cytometry. The expression levels of ROS-associated NAD(P)H subunits gp91 and p47, the anti-oxidative enzyme catalase (CAT), Nrf2, heme oxygenase-1 (HO-1) and endothelial nitric oxide synthase (eNOS) were detected by Western blotting. The results showed that CYP2C8-derived EETs decreased apoptosis of HUVECs treated with TNF-α. Pretreatment with 11, 12-EET also significantly blocked TNF-α-induced ROS production. In addition, 11, 12-EET decreased oxidative stress-induced apoptosis. Furthermore, the ability of 11, 12-EET to protect cells against TNF-α-induced apoptosis via oxidative stress was abrogated by transient transfection with Nrf2-specific small interfering RNA (siRNA). In conclusion, CYP2C8-derived EETs prevented TNF-α-induced HUVECs apoptosis via inhibition of oxidative stress associated with the Nrf2 signaling.

8,11,14-Eicosatrienoic Acid , Metabolism , Pharmacology , Adaptor Proteins, Signal Transducing , Genetics , Metabolism , Apoptosis , Aryl Hydrocarbon Hydroxylases , Genetics , Metabolism , Atherosclerosis , Genetics , Metabolism , Pathology , Catalase , Genetics , Metabolism , Cytochrome P-450 CYP2C8 , Genetics , Metabolism , Gene Expression Regulation , Heme Oxygenase-1 , Genetics , Metabolism , Human Umbilical Vein Endothelial Cells , Cell Biology , Metabolism , Humans , Membrane Glycoproteins , Genetics , Metabolism , Models, Biological , NADPH Oxidase 2 , NADPH Oxidases , Genetics , Metabolism , NF-E2-Related Factor 2 , Genetics , Metabolism , Nitric Oxide Synthase Type III , Genetics , Metabolism , RNA, Small Interfering , Genetics , Metabolism , Reactive Oxygen Species , Metabolism , Signal Transduction , Tumor Necrosis Factor-alpha , Metabolism , Pharmacology
Article in English | WPRIM | ID: wpr-305461


<p><b>OBJECTIVE</b>To observe the effects of retinoic acid (RA) on the proliferation and differentiation of a human ovarian carcinoma cell line: 3AO cells.</p><p><b>METHODS</b>3AO cell proliferation was evaluated by viable cell count, percentage of cells in each cycle phase were analyzed by flow cytometric analysis, alkaline phosphatase (AKP) activity was determined as described, and CA125 expression was measured by ELISA.</p><p><b>RESULTS</b>RA could inhibit the proliferation of 3AO cells accompanied with morphological changes in a dose-dependent manner. Cell cycle analysis indicated that RA inhibition of 3AO cells growth occurred through induction of G1 arrest with a concomitant reduction in the proportion of cells in S phase, AKP activity increased significantly after treatment with RA (0.1 micromol/L) for 1-5 days. Dose-response studies revealed that the AKP activity increased to a different extent as a function of RA concentrations. Furthermore, RA could suppress the expression of CA125 tumor marker in 3AO cells.</p><p><b>CONCLUSION</b>RA could markedly inhibit the proliferation and induce the differentiation of 3AO cells.</p>

Alkaline Phosphatase , Metabolism , Antineoplastic Agents , Pharmacology , Biomarkers, Tumor , Cell Cycle , Cell Differentiation , Cell Line, Tumor , Cell Proliferation , Dose-Response Relationship, Drug , Female , Humans , Ovarian Neoplasms , Metabolism , Pathology , Proteins , Metabolism , Tretinoin , Pharmacology
Article in Chinese | WPRIM | ID: wpr-680415


Objective:To investigate the mechanism responsible for lost sensibility of tyrosine aminotransferase(TAT)to dexam- ethasone(Dex)in human hepatoma cell line SMMC-7721 through examining the cDNA sequence of TAT and the status of glucocorticoid receptor(GR)pathway.Methods:The TAT cDNA fragment containing the full length of coding sequence was amplified by reverse transcription-polymerase chain reaction(RT-PCR)and was sequenced.The expression of TAT mRNA was determined by real-time quantitative PCR to observe the influence of Dex on expression of TAT mRNA in SMMC-7721 cells.The experiement with HepG2 cells was performed as the control.Reporter genes(GRE-tk-LUC and GRE-MMTV-CAT)were transiently transfected into SMMC-7721 cells by electroporation.The induction efficiencies of LUC and CAT genes expression by Dex were examined and compared between SMMC-7721 cells and HepG2 cells.Results:The results showed that there was a same-sense mutation(Gln576Gln)in TAT cDNA se- quence.TAT mRNA could be induced by Dex,with the maximal induction level being 2.22-folds in SMMC-7721 cells,which was signifi- cantly lower than that in HepG2 cells(15.1-fold increase,P