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1.
Article in Chinese | WPRIM | ID: wpr-939526

ABSTRACT

OBJECTIVE@#To observe the clinical effect of moxibustion combined with plucking technique at Jiquan (HT 1) for preventing peripherally inserted central catheter (PICC)-related venous thrombosis in the upper limbs of malignant tumor patients.@*METHODS@#A total of 80 malignant tumor patients undergoing PICC were randomized into an observation group and a control group, 40 cases in each one. In the control group, the routine care for PICC was exerted. In the observation group, besides the routine care, moxibustion combined with plucking technique at Jiquan (HT 1) was added. Mild moxibustion was exerted along the venous distribution of PICC (avoiding the entry site) for 10 to 15 min, and then, the circling moxibustion was applied to Quchi (LI 11), Xuehai (SP 10) and Tianfu (LU 3), 3 to 5 min at each acupoint. Finally, plucking technique was given at Jiquan (HT 1) for 5 to 10 min. This combined therapy was intervened since the 2nd day of PICC placement, once daily, 5 times a week, for 3 weeks totally. The incidence of the PICC-related venous thrombosis in the upper limbs was compared between the two groups on day 42 of placement. On day 2, 7, 14, 21, 28, 35 and 42 of PICC placement, the peak systolic velocity (PSV) and the end-diastolic velocity (EDV) of the subclavicular vein on the placement side were observed separately in the two groups.@*RESULTS@#The incidence of the PICC-related venous thrombosis in the upper limbs in the observation group was lower than that in the control group (2.5% [1/40] vs 17.5% [7/40], P<0.05). From day 7 to 35 of PICC placement, PSV of the subclavicular vein on the placement side was higher than that on the day 2 of PICC placement in the observation group (P<0.05). On day 28 and 42 of PICC placement, PSV of the subclavicular vein on the placement side was lower than that on the day 2 of PICC placement in the control group (P<0.05). In the observation group, EDV of the subclavicular vein on the placement side was higher than that on the day 2 of PICC placement from day 7 to 28 of PICC placement (P<0.05). In the control group, EDV of the subclavicular vein on the placement side from day 28 to 42 of PICC placement was lower than that on the day 2 of PICC placement (P<0.05). From day 7 to 42 of PICC placement, PSV and EDV of the subclavicular vein on the placement side in the observation group were all higher than those in the control group (P<0.01, P<0.05).@*CONCLUSION@#The combined treatment of moxibustion with plucking technique at Jiquan (HT 1) can effectively prevent PICC-related venous thrombosis in the upper limbs and improve venous blood flow velocity in malignant tumor patients.


Subject(s)
Humans , Catheterization, Central Venous/methods , Catheterization, Peripheral/adverse effects , Moxibustion/adverse effects , Neoplasms/complications , Upper Extremity , Venous Thrombosis/etiology
2.
Military Medical Sciences ; (12): 53-56, 2018.
Article in Chinese | WPRIM | ID: wpr-694315

ABSTRACT

Objective To establish an Ampliseq method that combines target-enrichment and the next-generation sequencing for simultaneous detection of Staphylococcus aureus, S.epidermidis, Klebsiella pneumoniae and Listeria monocytogenes in order to provide a fast and accurate means to detect pathogens in bloodstream infections.Methods A method to evaluate the LOD,specificity and sensitivity by constructing simulated samples spiked in known pathogens was established.Results and Conclusion Target-enrichment Ampliseq showed good sensitivity and specificity,and the limit of detection(LOD)was as low as 101CFU/ml.The sensitivity was 95.38%,the specificity was 95.45%and the Kappa value was between 0.839 -1.000.This method can detect S.aureus,S.epidermidis,K.pneumoniae and L.monocytogenes simultaneously in one reaction within 15 hours.

3.
Military Medical Sciences ; (12): 902-905, 2017.
Article in Chinese | WPRIM | ID: wpr-694278

ABSTRACT

Objective To develop a PCR-array method for detecting common purulent meningitis pathogens including Streptococcus pneumoniae,Escherichia coli,Haemophilus influenzae type B,Neisseria meningitidis,S.agalactiae and Listeria monocytogenes in children.Methods The amplification efficiency,limit of detection (LOD) and cross-reactivity were validated with individually real-time PCR using genomic DNA of the six pathogenic bacteria.The sensitivity and specificity of the PCR-array method were evaluated using artificial cerebrospinal fluid(CSF),and the consistency between the PCR-array method and the golden method of CSF culture was evaluated using clinical samples.Results The primers and probes of the pathogens in PCR-array had high specificity,and there was no cross reaction between them.The LOD of the PCR-array method was 10 cfu/ml and very sensitive.The sensitivity and specificity of the PCR-array method could reach 95% in the evaluation of artificial CSF,and had a good consistency with the clinical gold standard method.Conclusion The PCR-array method with high sensitivity and specificity can simultaneously detect six common pathogens in children with purulent meningitis at 2.5 h,which could provide reference for the diagnosis of purulent meningitis.

4.
Article in Chinese | WPRIM | ID: wpr-336075

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of ginsenoside Rg3 on the apoptosis and survivin expression in human lung squamous cell carcinoma cell line SK-MES-1.</p><p><b>METHODS</b>SK-MES-1 cells were divided into Rg3 treatment group, blank control group and positive control (arsenic trioxide) group. The apoptotic rate of the cells in each group was determined using flow cytometry, and the expression of survivin protein and mRNA was detected by immunocytochemistry and RT-PCR, respectively.</p><p><b>RESULTS</b>A 48-h treatment with Ginsenoside Rg3 induced increased apoptotic rate of SK-MES-1 cells in a dose-dependent manner. Ginsenoside Rg3 significantly downregulated the expressions of survivin protein and mRNA as compared with the expression levels in the blank control group (P<0.05).</p><p><b>CONCLUSION</b>Ginsenoside Rg3 can induce the apoptosis of SK-MES-1 cells, the mechanism of which may involve inhibited survivin expression.</p>


Subject(s)
Humans , Apoptosis , Carcinoma, Squamous Cell , Metabolism , Pathology , Cell Line, Tumor , Down-Regulation , Ginsenosides , Pharmacology , Inhibitor of Apoptosis Proteins , Lung Neoplasms , Metabolism , Pathology , Microtubule-Associated Proteins , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism
5.
Chinese Journal of Biotechnology ; (12): 456-460, 2005.
Article in Chinese | WPRIM | ID: wpr-305251

ABSTRACT

In order to construct RGD-mSAK mutant with reduced immunogenicity, and identify its biological activity after purification, mSAK gene fragment was amplified by over-lapping extension PCR. Then the gene was inserted into the prokaryotic expression vector pBV220 with P(R)P(L) promoters after confirmed by DNA sequencing; the expression plasmid pBV220-RGD-mSAK was constructed, and then was transformed into E. coli. DH5alpha. After temperature induction, the mutant Staphylokinase was over-expressed and much of protein was in the supernate of lysate, which is over 50% of total protein in the host. The protein was isolated and purified in Q-Sepharose FF, Sephacryl S-200 and SP, high purity protein was obtained and its purity was over 98%. The thrombolysis activity of the RGD-mSAK protein is 1.68 x 10(5) u/mg by fibrin plate assay, which is slightly higher than that of the wild-type, and antiserum titers raised against this protein in guinea pigs were much lower than those of wild-type SAK, determined by ELISA. In anti-platelets aggregation assay in vitro, the RGD-mSAK protein has obvious inhibition activity of platelet aggregation in low concentration comparing to the control group and wild-type SAK group. So the RGD-mSAK protein is a low immunogenicity, bi-function molecular with both thrombolysis activity and anti-embolism activity. It provided the basis for further research of RGD-SAK.


Subject(s)
Animals , Base Sequence , Escherichia coli , Genetics , Metabolism , Guinea Pigs , Metalloendopeptidases , Metabolism , Molecular Sequence Data , Mutant Proteins , Genetics , Oligopeptides , Metabolism , Platelet Aggregation , Platelet Aggregation Inhibitors , Pharmacology , Protein Engineering , Recombinant Proteins , Genetics
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