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1.
Article in English | WPRIM | ID: wpr-937009

ABSTRACT

@#Cardiovascular implantable electronic devices (CIEDs) are widely used in the modern era. Every year, about 730,000 permanent pacemakers and 330,000 CIEDs are implanted worldwide. CIEDs have been known to increase the life expectancy of millions of people and improve their quality of life by controlling the heart rate and atrioventricular and interventricular synchronization and preventing sudden cardiac death.[1] The tricuspid valve consists of the annulus, leaflets, chordae tendineae, and papillary muscles. Interaction between the endocardial lead and any component of this structure can lead to tricuspid valve dysfunction, thereby resulting in tricuspid regurgitation (TR).[2] CIED-related TR has been shown to be an independent predictor of hospitalization for heart failure.[3,4]

2.
Article in Chinese | WPRIM | ID: wpr-936452

ABSTRACT

Objective To analyze the epidemiological characteristics of elderly hospitalized patients with bronchial asthma in Northern Theater Command General Hospital, and to provide reference for the prevention and treatment of elderly hospitalized bronchial asthma. Methods Clinical data of 724 hospitalized patients with bronchial asthma in Northern Theater Command General Hospital were retrospectively analyzed, and general data of 724 patients with bronchial asthma were collected. According to the age of the patients, they were divided into the elderly group (≥60 years old, n=308) and the non-elderly group (< 60 years old, n=416) for analysis and comparison. Multivariate logistic regression was used to analyze the influencing factors of asthma control and attack severity in the elderly. Results There were 308 elderly patients, including 128 males (41.56%) and 180 females (58.44%). The control of asthma was good in 108 cases (35.06%), general in 136 cases (44.16%) and poor in 28 cases (9.09%). The severity of asthma attack was mild in 16 cases (51.95%), moderate in 184 cases (59.74%), severe in 92 cases (29.87%), and critical in 0 cases (0.00%). There were 416 non-elderly patients, including 224 males (53.85%) and 192 females (46.15%). The asthma control in the non-elderly patients was good in 40 cases (9.62%), general in 140 cases (33.65%), and poor in 132 cases (31.73%). The severity of asthma attack was mild in 100 cases (24.04%), moderate in 208 cases (50.00%), severe in 72 cases (17.31%), and critical in 4 cases (0.96%). There were statistically significant differences between the two groups in gender, smoking status, obesity, education level, basic diseases, asthma control and asthma attack severity (P<0.05). The average age, average hospitalization cost, average length of stay and average course of disease in the elderly group were significantly higher than those in the non-elderly group (P<0.05). Logistic regression analysis showed that age, obesity, and education level were independent risk factors for asthma control (P<0.05). Conclusion The prevalence of elderly asthma patients in Northern Theater Command General Hospital region is high and the condition control is general. Age is an independent risk factor affecting the control of the disease condition and the severity of attacks in elderly asthma patients. In addition, obesity and education level are independent risk factors affecting the control of asthma in elderly patients.

3.
Acta Pharmaceutica Sinica ; (12): 514-524, 2022.
Article in Chinese | WPRIM | ID: wpr-922928

ABSTRACT

Rhei Rhizoma is commonly used as a traditional Chinese medicine with multiple botanical origins. Different botanical sources may have different pharmacological activities. The germplasm resources of commercial Rhei Rhizoma were determined based on the chloroplast gene matK, and the anthraquinone and free anthraquinone content was determined by UPLC to analyze quality of commercial Rhei Rhizoma. Eighty-nine commercial Rhei Rhizoma samples were collected from 40 cities in 27 provinces. DNA was extracted and the matK gene was amplified by PCR. Results indicated that the collected samples were from the same botanical origin, Rheum palmatum, and 8 genotypes were identified, including Rp1, Rp2, Rp3, Rp4, Rp5, Rp6, Rp10 and Rp12. Rp4 and Rp6, cultivated in Gansu, Sichuan and Yunnan provinces were the main circulating genotypes, representing 40.45% and 37.08% of the total samples, respectively. Phylogenetic tree analysis showed that the eight genotypes were mainly divided into two branches, of which the main genotypes Rp4 and Rp6 were in one branch. Genetic distance analysis indicated that the genetic separation of the eight genotypes was between 0.001 and 0.010. UPLC analysis indicated that 93.26% of the samples met the Pharmacopoeia standards. There were significant differences in the content of total anthraquinone and free anthraquinone among the samples, in which the difference in free anthraquinone was 1.01% and the difference in total anthraquinone content was 3.79%, indicating that the quality of commercial Rhei Rhizoma samples varies considerably. There was no significant difference in the content of total anthraquinone and free anthraquinone in commercial Rhei Rhizoma among different collection provinces and genotypes. This study will help guide the circulation of Rhei Rhizoma in the market and provides valuable insights for molecular identification and quality analysis of other traditional Chinese medicines.

4.
Acta Pharmaceutica Sinica ; (12): 385-391, 2022.
Article in Chinese | WPRIM | ID: wpr-922909

ABSTRACT

The potential application of dendritic cells (DC) sensitized with cytosine-phosphoric acid-guanine (CpG) oligodeoxynucleotide (ODN) and tumor antigen as a vaccine against murine melanoma was investigated with freshly isolated mouse bone marrow-derived dendritic cells. For the DC vaccine preparation, DC were sensitized with the B16 tumor antigen and CpG ODN was used to promote further maturation of the DC. The immunogenic activity of the vaccine was evaluated in vitro by determining the proliferation of T lymphocytes and the killing effect of cytotoxic T lymphocytes (CTL) on B16 tumor cells. The DC vaccine was injected intraperitoneally and tumor inhibition in mice bearing B16 xenografts was examined. All mice were cared for under an approved SIMM Institutional Animal Care and Use Committee (IACUC) protocol. In vitro, this DC vaccine promoted the proliferation of T lymphocytes and showed a potent killing effect on the target B16 cells. In vivo experiments showed that after treatment or pre-immunization both the tumor volume and weight were significantly decreased. The DC vaccine with CpG ODN and tumor antigen exhibited an inhibitory effect against melanoma, providing a potential method for melanoma cancer treatment.

5.
Article in English | WPRIM | ID: wpr-929012

ABSTRACT

N6-methyladenosine (m6A) methylation modification is one of the most common epigenetic modifications for eukaryotic mRNA. Under the catalytic regulation of relevant enzymes, m6A participates in the body's pathophysiological processes via mediating RNA transcription, splicing, translation, and decay. In the past, we mainly focused on the regulation of m6A in tumors such as hematological tumors, cervical cancer, breast cancer. In recent years, it has been found that m6A is enriched in mRNAs of neurogenesis, cell cycle, and neuron differentiation. Its regulation in the nervous system is gradually being recognized. When the level of m6A modification and the expression levels of relevant enzyme proteins are changed, it will cause neurological dysfunction and participate in the occurrence and conversion of neurological diseases. Recent studies have found that the m6A modification and its associated enzymes were involved in major depressive disorder, Parkinson's disease, Alzheimer's disease, Fragile X syndrome, amyotrophic lateral sclerosis, and traumatic brain injury, and they also play a key role in the development of neurological diseases and many other neurological diseases. This paper mainly reviewed the recent progress of m6A modification-related enzymes, focusing on the impact of m6A modification and related enzyme-mediated regulation of gene expression on the central nervous system diseases, so as to provide potential targets for the prevention of neurological diseases.


Subject(s)
Adenosine/metabolism , Depressive Disorder, Major , Epigenesis, Genetic , Humans , Methylation , RNA, Messenger/metabolism
6.
Article in Chinese | WPRIM | ID: wpr-940552

ABSTRACT

The increasing incidence of obesity and diabetes has made diabetic kidney disease (DKD) the main cause of chronic kidney disease and end-stage renal disease. Despite current pharmacological interventions for blood glucose control and renin-angiotensin system inhibition, the risk of kidney disease progression and complications remains high. At present, the pathogenesis of DKD has been clarified to be related to chronic inflammatory response, oxidative stress, glucose and lipid metabolism disorders, and hemodynamic abnormalities. According to recent studies, the programmed cell deaths (PCD) of renal intrinsic cells such as pyroptosis and necroptosis play a key role in the occurrence and development of DKD. Pyroptosis and necroptosis, the two newly discovered routes of PCD, can protect the hosts from being invaded by microbial pathogens, but their dysregulation is associated with multiple autoimmunity and autoinflammatory responses. Pyroptosis and necroptosis are closely interlinked and cross-regulated. Different from apoptosis, these two cellular suicide mechanisms cause membrane rupture and release of cell contents through their respective gasdermin D (GSDMD) and mixed lineage kinase domain-like protein (MLKL), with damage-associated molecular patterns (DAMPs) and inflammatory cytokines like interleukin-1β (IL-1β) involved to trigger inflammation, and chronic inflammatory responses are key factors leading to the progression of DKD. Traditional Chinese medicine (TCM) has long been employed for the prevention and treatment of DKD and the resulting clinical outcomes are remarkable. TCM has been proved to exert a protective effect against DKD by affecting the expression of nucleotide oligomerization domain-like receptor protein 3 (NLRP3) inflammasome, receptor-interacting protein kinase 3 (RIPK3), and MLKL. This paper reviewed the relationship of pyroptosis and necroptosis with DKD and its intervention with TCM.

7.
Article in Chinese | WPRIM | ID: wpr-940440

ABSTRACT

Diabetic nephropathy (DN) is one of the serious microvascular complications of diabetes mellitus (DM),and is the main cause of end-stage renal disease(ESRD) worldwide. Although lowering blood glucose and,lowering blood pressure and blocking the renin-angiotensin-aldosterone system(RAAS) can reduce blood glucose,blood pressure and urinary protein to a certain extent,it is still difficult to prevent the progression of DN sometimes. The curative effect of traditional Chinese medicine on DN has been confirmed,but its mechanism is has not been fully clarified. Autophagy is a highly conserved lysosomal degradation pathway in mammals that removes protein aggregates and damaged organelles to maintain cell homeostasis. Podocyte,also known as glomerular epithelial cells,is an important component in maintaining the homeostasis of glomerular filtration barrier,and podocyte injury is considered to be a central link in the occurrence and development of DN. As a highly differentiated cell,podocyte maintains a high level of autophagy to maintain its homeostasis under physiological conditions. In DN state,mammalian target of rapamycin (mTOR),AMP-activated protein kinase (AMPK),silent information regulator 1(SIRT1) and other nutritional signaling pathways as well as intracellular stress response signaling pathways such as oxidative stress,endoplasmic reticulum stress ,and hypoxia stress,etc.,affect podocyte autophagy of podocytes, and ultimately leading to podocyte injury and the progression of DN. In recent years,regulation of podocyte autophagy has become one of the hot spots in DN research,and has also received extensive attention in the field of Chinese medicine. A review and summary of the domestic and international literature in this field reveals that Chinese medicine can affect podocyte autophagy in multiple pathways and targets. Nevertheless, those studiesbut mainly focuses on two nutrient-sensing signaling pathways,mTOR and AMPK,and there lacks more comprehensive and in-depth mechanism studies. In addition,the current research mainly concentrates focuses on the field of Chinese medicine monomers and Chinese medicine compounds,and rarely studies multi-component Chinese medicinelacking research on Chinese medicine component drugs and single drugs,and the research still lacks there is a lack of hierarchy. The regulatory mechanism of Chinese medicine on podocyte autophagy of podocytes in DN state in Chinese medicine still needs to be further studied in depth and systematically.

8.
Article in Chinese | WPRIM | ID: wpr-940433

ABSTRACT

ObjectiveTo preliminarily predict the active components, targets, and signaling pathways of modified Shengjiangsan in the treatment of immunoglobulin A nephropathy (IgAN) based on network pharmacology, and to explore its underlying mechanism through molecular docking and experimental verification on animals. MethodThe active ingredients and related targets of modified Shengjiangsan were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP), UniProt, SwissTargetPrediction, and literature review. IgAN-related targets were obtained from GeneCards and Online Mendelian Inheritance in Man (OMIM). Cytoscape 3.9.0 was used to construct the regulation network of the related targets of Shengjiangsan and IgAN, and the protein-protein interaction (PPI) network was plotted by STRING. The common genes were analyzed for gene ontology (GO) functional annotation and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment by Metascape. Key targets and main active ingredients were selected for molecular docking by AutoDockTools 1.5.6. The experimental model of IgAN was induced by bovine serum albumin(BSA, ig) combined with lipopolysaccharide (LPS, iv) and the complex of CCl4 and castor oil (sc) in rats. The model rats were treated with modified Shengjiangsan and benazepril hydrochloride for four weeks. The rats were sacrificed after drug administration. The levels of transforming growth factor-β1 (TGF-β1) and interleukin-6 (IL-6) in the serum and kidney tissues were detected by enzyme-linked immunosorbent assay(ELISA), immunohistochemistry, Real-time quantitative polymerase chain reaction (Real-time PCR), and Western blot. ResultA total of 105 active ingredients were obtained according to oral bioavailability(OB), drug-likeness(DL), and literature screening. There were 124 common genes and 59 core targets. Neurotrophic tyrosine receptor kinase 1 (NTRK1), cullin-3 (CUL3), tumor protein 53 (TP53), epidermal growth factor receptor (EGFR), exportin 1 (XPO1), and other targets might be closely related to IgAN. As predicted by KEGG enrichment analysis, the treatment of IgAN with modified Shengjiangsan mainly involved the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway, nuclear transcription factor-kappa B (NF-κB) signaling pathway, and cytokine-cytokine receptor interaction signaling pathway. As revealed by molecular docking, the main active ingredients in modified Shengjiangsan showed stable binding activities with NTRK1, CUL3, TP53, EGFR, and XPO1 in the core targets, indicating that it presumedly regulated inflammatory responses by affecting NTRK1, CUL3, TP53, EGFR, and XPO1 target proteins. The results of experimental verification on animals showed that the expression levels of cytokines TGF-β1 and IL-6 in the serum and kidney tissues of IgAN rats were significantly decreased by modified Shengjiangsan, suggesting that Shengjiangsan might inhibit excessive fibrosis, and inflammatory and immune responses by regulating signaling pathways such as cytokine-cytokine receptor interaction, PI3K/Akt, and NF-κB. ConclusionModified Shengjiangsan may treat IgAN through multiple targets and pathways. Its mechanism may be related to the inhibition of excessive fibrosis, and inflammatory and immune responses by affecting the expression of NTRK1, CUL3, TP53, EGFR, and XPO1 and the regulation of the cytokine-cytokine receptor interaction, PI3K/Akt, NF-κB, and other signaling pathways.

9.
Article in Chinese | WPRIM | ID: wpr-940285

ABSTRACT

ObjectiveTo study the effects of Wendantang on the expression of miRNA-219, N-methyl-D-aspartate receptor 2B (NR2B), disrupted in schizophrenia 1 (DISC1), and Ca2+/calmodulin-dependent protein kinase Ⅱγ (CaMKⅡγ) in the frontal lobe of rats with schizophrenia. MethodSixty rats were randomly divided into six groups, namely normal group, model group, high-, medium-, and low-dose Wendantang groups, and clozapine group, with 10 rats in each group. Rats in high-, medium-, and low-dose Wendantang groups were intragastric with 40, 20, and 10 g·kg-1 Wendantang, and the ones in clozapine group were intragastric with 0.02 g·kg-1 clozapine, those in normal and model group were intragastric with equal volume of normal saline, once a day. After 21 days of administration, rats in all groups except for the normal group were injected with 0.6 mg·kg-1 dizocilpine maleate (MK-801) into the left abdominal cavity for inducing acute schizophrenia. The stereotypic behavior and ataxia in rats were scored according to SAMS and HOFFMAN criteria. The morphological changes in the prefrontal cortex were observed by hematoxylin-eosin (HE) staining. The protein expression levels of NR2B, DISC1 and CaMKⅡγ in the frontal lobe was detected by Western blot. The mRNA expression levels of miRNA-219 was detected by real-time fluorescence quantitative polymerase chain reaction(Real-time PCR). ResultCompared with normal group, the model group exhibited significantly increased stereotypic behavior and ataxia scores (P<0.01), karyopyknosis and karyolysis in most neurons of the prefrontal cortex, and down-regulated NR2B, DISC1, and CaMKⅡγ protein expression (P<0.01) and miRNA-219, NR2B, DISC1, and CaMKⅡγ mRNA expression (P<0.01). Compared with model group, Wendantang high-, medium-, and low-doses group lowered the scores of stereotypic behavior and ataxia at 50, 60 mmin(P<0.05,P<0.01). In high- and medium-dose Wendantang groups, the neurons in the prefrontal cortex were densely arranged. The karyopyknosis and karyolysis were alleviated to varying degrees. The NR2B protein expression in the frontal lobe was up-regulated (P<0.01). In the medium- and low-dose Wendantang groups, the DISC1 protein expression in the frontal lobe was up-regulated (P<0.05,P<0.01). Wendantang at each dose significantly increased the CaMKⅡγ protein expression (P<0.05) and miRNA-219, NR2B, DISC1, and CaMKⅡγ mRNA expression in the frontal lobe (P<0.05,P<0.01). ConclusionWendantang improves the scores of stereotypical behavior and ataxia, relieves the karyopyknosis and karyolysis of neurons in the prefrontal cortex, and increases the expression levels of miRNA-219, NR2B, DISC1, and CaMKⅡγ of rats with schizophrenia, so as to alleviate the schizophrenic-like symptoms and schizophrenia.

10.
Chinese Journal of Oncology ; (12): 238-245, 2022.
Article in Chinese | WPRIM | ID: wpr-935206

ABSTRACT

Objective: To investigate the molecular mechanism of circZNF609 targeting miR-153 to regulate the proliferation and apoptosis of diffuse large B-cell lymphoma. Methods: Fifty cases of lymphoma tissue from patients with diffuse large B-cell lymphoma who were diagnosed and treated in the First Affiliated Hospital of Zhengzhou University from July 2018 to December 2019 were collected. Thirty cases of normal lymph node tissues that were confirmed to be reactive hyperplasia by pathological diagnosis during the same period were selected as controls. Real time quantitative polymerase chain reaction (PCR) was used to detect the expression of circZNF609 in diffuse large B-cell lymphoma tissues and control hyperplasia lymph nodes. Diffuse large B-cell lymphoma OCI-LY19 cells were divided into control group (blank control), si-con group (transfected with siRNA control), si-ZNF609 group (transfected with circZNF609 siRNA), and si-ZNF609+ Anti-NC group (co-transfected with circZNF609 siRNA and inhibitor control) and si-ZNF609+ Anti-miR-153 group (co-transfected with circZNF609 siRNA and miR-153 inhibitor). Cell counting kit-8 (CCK-8) was used to detected proliferation, flow cytometry was used to detect cell cycle and apoptosis. Western blot was used to detect the protein expressions of C-caspase-3, cyclin D1, p21. The luciferase reporter system was used to identifie the relationship between circZNF609 and miR-153. Results: The expression level of circZNF609 in diffuse large B-cell lymphoma tissue was (1.44±0.22), higher than (0.37±0.14) in the control tissues (P<0.001). The cell survival rate of the si-ZNF609 group was (51.74±6.39)%, lower than (100.00±10.23)% of the control group and the (99.64±11.67)% of the si-con group (P<0.001). The proportion of cells in the G(0)/G(1) phase was (63.25±4.11)%, higher than (48.62±4.32)% of the control group and (47.12±3.20)% of the si-con group (P<0.001), the apoptosis rate was (13.36±1.42)%, higher than (3.65±0.47)% of the control group and (3.84±0.62)% of the si-con group (P<0.05). The expression levels of C-caspase-3 and p21 protein were (0.85±0.09) and (0.90±0.08), higher than (0.38±0.04) and (0.65±0.07) in the control group and (0.39±0.05) and (0.66±0.05) in the si-con group (P<0.001). The expression level of cyclin D1 protein was (0.40±0.03), lower than (0.52±0.06) of the control group and (0.53±0.04) of the si-con group (all P<0.001). CircZNF609 and miR-153 are mutually targeted. The cell survival rate of the si-ZNF609+ Anti-miR-153 group was (169.92±13.25)%, higher than (100.00±9.68)% of the si-ZNF609+ Anti-NC group (P<0.001), the ratio of cells in G(0)/G(1) phase and apoptosis rate were (52.01±3.62)% and (8.20±0.87)%, respectively, lower than (64.51±5.17)% and (14.03±1.17)% in the si-ZNF609+ Anti-NC group (P<0.001). The protein expression levels of C-caspase-3 and p21 were (0.42±0.06) and (0.52±0.06), lower than (0.80±0.07) and (0.92±0.10) of the si-ZNF609+ Anti-NC group (P<0.001). The protein expression level of cyclin D1 was (0.68±0.07), higher than (0.39±0.04) in the si-ZNF609+ Anti-NC group (P<0.001). Conclusion: Down-regulation of circZNF609 inhibits the proliferation of diffuse large B-cell lymphoma OCI-LY19 cells and induces apoptosis by targeting miR-153.


Subject(s)
Apoptosis/genetics , Cell Line, Tumor , Cell Proliferation/genetics , Gene Expression Regulation, Neoplastic , Humans , Lymphoma, Large B-Cell, Diffuse/pathology , MicroRNAs/genetics , RNA, Circular/genetics
11.
Article in Chinese | WPRIM | ID: wpr-933985

ABSTRACT

Objective:To analyze and compare rehabilitation research funded by China′s National Natural Science Foundation (NSFC) with that funded by America′s National Institutes of Health (NIH) so as to provide references for future funding.Methods:Articles reporting rehabilitation research funded by the NSFC and the NIH were retrieved from the NSFC′s Science Output Service website and the NIH′s Project Report website and analyzed.Results:From 2009 to 2018 the NSFC funded 421 rehabilitation studies which resulted in a published report while the NIH funded 312. In 2018, the NSFC budget (US$3.89 million) was 8.46 times that of 2009 (US$460, 000), while the NIH′s grant budget (US$36.08 million) was 2.17 times that of 2009 (US$16.62 million). The number of published papers resulting from the Chinese and American studies was 1111 and 2571 respectively. Their impact factors mainly ranged between 0 and 3 points. Among the journals with an impact factor of 6 or more, published papers from the United States (297) were much more numerous than those from China (18). The number of SCI papers per million US dollars increased by 2.25 times in China and 0.05 times in the US.Conclusions:Both China and the United States have been investing more in rehabilitation medicine research, and that has resulted in more published papers. There is still a gap in funding and output between the two countries.

12.
Journal of Chinese Physician ; (12): 383-386, 2022.
Article in Chinese | WPRIM | ID: wpr-932074

ABSTRACT

Objective:To examine the influence of acute hypoxemia on central venous pressure (CVP) and diastolic blood pressure (DBP) in critical patients assisted by mechanical ventilation.Methods:We retrospectively analyzed the clinical data of critical patients assisted by mechanical ventilation in Medical Information Mart for Intensive Care Ⅲ (MIMIC-Ⅲ) database. Influence of acute hypoxemia on CVP and diastolic blood pressure (DBP) were evaluated. Hypoxemia was defined according to oxygenation index (OI) (OI≤100 as severe, 100<OI≤200 as moderate). Two cutoff values were set at OI=100 and OI=200. The primary outcomes were the difference between mean CVP, mean DBP 6 hours after the onset of hypoxemia and 6 hours before the event.Results:Among all critical patients assisted by mechanical ventilation, 508 patients met criteria of severe hypoxemia, and 1 117 patients met criteria of moderate hypoxemia. After adjusting positive expiratory end pressure (PEEP) and heart rate by multiple linear regression, CVP in patients with moderate and severe hypoxia increased significantly during the observation window of acute hypoxemia ( P=0.04, 0.02), but DBP did not change significantly ( P=0.29, 0.31). Conclusions:Acute hypoxemia could increase CVP and probably pulmonary circulation resistance in respiratory failure patients.

13.
Article in Chinese | WPRIM | ID: wpr-931191

ABSTRACT

Objective:To explore the application value of non-invasive prenatal testing (NIPT) technology in twin pregnancy.Methods:A total of 339 twin pregnant women who underwent NIPT at Dalian Municipal Women and Children′s Medical Center(Group), Dalian Jinpu New District Maternity and Child Health Hospital, and Dalian Lvshunkou District People′s Hospital from July 1, 2019 to June 30, 2021 were continuously retrospectively included. The clinical characteristics and test results of pregnant women with high-risk and low-risk were analyzed.Results:Among 339 pregnant women, 336 were successfully tested, with a success rate of 99.12%(336/339); 6 pregnant women were at high risk of NIPT, with a positive screening rate of 1.77%(6/339), including 1 case of high risk of trisomy 13, 2 cases of high risk of trisomy 18, and 3 cases of high risk of Trisomy 21; the results of amniocentesis for 2 high-risk pregnant women were not abnormal.Conclusions:NIPT technology is non-invasive, safe and efficient, and is suitable for large-scale prenatal screening. However, the detection accuracy of pregnant women with twin pregnancy needs to be improved.

14.
Article in Chinese | WPRIM | ID: wpr-929971

ABSTRACT

Head and neck squamous cell carcinoma is the sixth most common malignant carcinoma worldwide, about 60% of patients are in advanced stage at the time of visit, the incidence rate is increasing gradually while the 3-year-survival rate has not been improved .Since most patients have reached advanced stage at the time of diagnosis, it brings great difficulties to treatment and leads to poor prognosis. In recent years, many articles have found out that Tertiary lymphoid structures are in and around samples of HNSCC patients and are positively related to disease progession and outcome of patients. In-depth analysis of TLS in the formation of HNSCC and the function in tumor micro environment can help estimate the prognosis of patients and provide guidance for future treatment In this article, the compositions, functions, current research of TLS with its prognostic factors and its influence on future treatment in HNSCC will be reviewed.

15.
Chinese Journal of Hematology ; (12): 400-407, 2022.
Article in Chinese | WPRIM | ID: wpr-929627

ABSTRACT

Objective: To explore the differences in the biological effects of different expansion systems on natural killer (NK) cells, as well as the safety and preliminary clinical efficacy in the treatment of patients with recurrence after allogeneic hematopoietic stem cell transplantation (allo-HSCT) . Methods: Peripheral blood cells from healthy donors were stimulated with either CD3 combined with CD52 or K562 feeder cells loaded with IL-21/4-1BB to induce NK cell expansion. Changes in the NK cell phenotype, cytokine secretion, and cytotoxicity before and after expansion were detected. We also evaluated the safety and clinical efficacy of two different expansion strategies for patients received NK infusion. Results: Compared with the CD3/CD52 monoclonal antibody amplification system, the feeder cell expansion group had a higher purity of NK cells and higher expression ratios of NK cell surface activation receptors such as DNAM-1 and NKp30, while inhibitory receptor CTLA-4 expression was low and NKG2D/CD25/CD69/ Trail/PD-1/TIM-3/TIGIT had no statistically significant differences between the groups. Further functional results showed that the expression level of KI67 in NK cells after expansion in the two groups increased significantly, especially in the feeder cell expansion group. Simultaneously, the perforin and granzyme B levels of NK cells in the feeder cell expansion group were significantly higher than in the CD3/CD52 expansion group. A retrospective analysis of eight patients who received monoclonal antibody-expanded NK cell reinfusion and nine patients with trophoblast cell-expanded NK cell reinfusion was done. The disease characteristics of the two groups were comparable, NK cell reinfusion was safe, and there were no obvious adverse reactions. Clinical prognostic results showed that in the CD3/CD52 monoclonal antibody amplification group, the MRD conversion rate was 50% (2/4) , and the feeder cell expansion group was 50% (3/6) . After 5 years of follow-up from allo-HSCT, three patients in the monoclonal antibody expansion group had long-term survival without leukemia, and the remaining five patients had died; two patients died in the feeder cell expansion group, and the other six patients had long-term survival. Six cases had GVHD before NK cell reinfusion, and GVHD did not aggravate or even relieved after NK cell reinfusion. Conclusions: Preliminary results show that the biological characteristics of NK cells with diverse expansion strategies are significantly different, which may affect the clinical prognosis of patients with recurrence or persistent minimal residual disease after HSCT. The two groups of patients treated with NK cells from different expansion strategies had no obvious adverse reactions after NK cell infusion, but efficacy still needs to be further confirmed.


Subject(s)
Antibodies, Monoclonal/pharmacology , Graft vs Host Disease/metabolism , Hematopoietic Stem Cell Transplantation , Humans , Killer Cells, Natural , Retrospective Studies , Treatment Outcome
16.
Article in Chinese | WPRIM | ID: wpr-920660

ABSTRACT

@#Perinatal depression, one of the most common complications in the perinatal period, has a significant impact on the physical and mental health of mothers and children.At present, it is difficult to diagnose perinatal depression at an early stage, so objective and effective biomarkers are of great significance for the early detection and treatment of perinatal depression. In recent years, the exploration of biomarkers for early diagnosis of perinatal depression has become a hot research topic, mainly in sex hormones, neuroendocrine-related hormones, immuno-inflammatory molecules, genetics, and epigenetics.This article reviews the research progress of the biomarkers of perinatal depression in recent years.

17.
Chinese Journal of School Health ; (12): 265-267, 2022.
Article in Chinese | WPRIM | ID: wpr-920630

ABSTRACT

Objective@#To investigate mental health status and associated factors among middle school students from single parent families in urban Lishui, and to provide reference for mental health education for adolescents from single parent families.@*Methods@#In the urban area of Lishui City, a total of 160 middle school students from single parent families in four middle schools were selected by stratified random sampling, among 160 middle school students from two parent families were selected matched for class, gender, age and family background, and Mental Health Diagnostic Test Scale (MHT) was used for testing.@*Results@#In terms of allergy tendency, physical symptoms, phobic tendency and total anxiety detection, middle school students from single parent families were higher than those from two parent families, and the differences were statistically significant ( χ 2=5.70, 5.34, 6.84, 4.30, P <0.05). Among the 160 middle school students from single parent families, the detection rate of girls in learning anxiety, anxiety towards others, self blame tendency, allergic tendency, physical symptoms, terror tendency, impulsive tendency and total anxiety were higher than that of boys, and the differences were statistically significant. ( χ 2=12.26, 4.61, 7.34, 14.96, 20.53, 10.05, 16.19 , 9.39, P <0.05). Multivariate Logistic regression analysis showed that gender was positively associated with mental health of middle school students in singleparent families ( OR=4.25, 95%CI=1.87-7.02, P <0.05).@*Conclusion@#There are a higher prevalence of psychological problems among middle school students from single parent families in Lishui city, especially among girls, which warrents further psychosocial services including mental health education.

18.
Article in Chinese | WPRIM | ID: wpr-928758

ABSTRACT

OBJECTIVE@#To investigate the changes of gene sequencing and proteomics of apheresis platelet (AP) exosomes in different storage periods and predict the function of AP exosomes in different storage periods.@*METHODS@#Platelets at different storage periods of 0 day (D0), 3 day (D3) and 5 day (D5) were collected, exosomes were extracted with Gradient centrifugation; gene sequencing and proteomic analysis were used to analyze the exosomes, and biological functions of platelet exosomes were analyzed and predicted by bioinformatics. Liquid mass spectrometry (LMS) was used to detect the changes and function prediction of exosomes proteins. The small RNA sequencing library was prepared, and the constructed library was sequenced and bioinformatics technology was used for data analysis.@*RESULTS@#AP exosome iTRAQ protein analysis showed that AP exosomes stored in D3 with 55 up-regulated proteins and 94 down-regulated proteins (P<0.05, FC<0.83 or FC>1.2), while AP exosomes stored in D5 with 292 up-regulated proteins and 53 down-regulated proteins (P<0.05, FC<0.83 or FC>1.2) as compared with D0. KEGG pathway analysis showed that the proteins were mainly involved in transport and metabolism, immune system, cancer, membrane transport and other processes. There were statistically significant differences between AP exosome miRNAs in different storage days (P<0.01). The number of miRNA up-regulated and down-regulated was 374 and 255 as compared with the number of platelet exosomes miRNA stored in D3 and D0, while that was 297 and 242 in D5 and D0, and 252 and 327 in D5 and D3, respectively. The target genes of differential platelet exosome miRNAs were analyzed by GO enrichment. Target genes of differential miRNA were mainly involved in membrane composition, mainly played molecular functions binding to proteins, and participated in biological processes of transcriptional regulation.@*CONCLUSION@#The exosome differential proteins and miRNAs in D5 are significantly different from those in the D0 of APs, and they are involved in various biological processes.


Subject(s)
Blood Component Removal , Blood Platelets/metabolism , Exosomes/metabolism , Humans , MicroRNAs/genetics , Proteomics
19.
Article in Chinese | WPRIM | ID: wpr-928738

ABSTRACT

OBJECTIVE@#To investigate the toxicity management and efficacy evaluation of BCMA-chimeric antigen receptor T cells(CART) in the treatment of relapsed and refractory multiple myeloma (MM).@*METHODS@#The efficacy and adverse reactions of 21 patients with MM who received BCMA-CART treatment at the First Affiliated Hospital of Wenzhou Medical University from December 2017 to September 2020 were evaluated, and the efficacy assessment and survival analysis for high-risk patients and non-high-risk patients were evaluated.@*RESULTS@#After infusion of BCMA-CART cells in 21 MM patients, the number of effective cases was 17, of which the complete remission (sCR/CR) was 10, and the partial remission (VGPR/PR) was 7. The median OS time for all patients was 19.4 months, and the median PFS time was 7.9 months. The number of patients with extramedullary disease(EMD), high-risk genetics, and ISS stage Ⅲ were 5, 15 and 8, and the effective number was 3, 11 and 6, respectively. The treatment of 3 patients without high-risk factors was effective. The median OS and median PFS of patients with EMD were 14.2 and 2.5 months, respectively, which were shorter than those of patients without EMD (19.4 months and 8.9 months, respectively). The median OS and median PFS of patients with high-risk cytogenetic factors and ISS Ⅲ were not significantly different from those of non-high-risk patients. Cytokine release syndrane (CRS) occurred in 20 patients, of which 14 cases were Grade 1 CRS, while 6 were Grade 2, no CRS of Grade 3 or above occurred. IL-6 receptor inhibitors were used in 9 patients. All CRS were controlled effectively, and no patients had neurological toxicity.@*CONCLUSION@#BCMA-CART is a certain curative effect in the treatment of relapsed and refractory multiple myeloma, and the adverse reactions can be well controlled through close monitoring and timely treatment.


Subject(s)
B-Cell Maturation Antigen , Humans , Immunotherapy, Adoptive/adverse effects , Multiple Myeloma/therapy , Receptors, Chimeric Antigen , Remission Induction
20.
Asian Journal of Andrology ; (6): 161-166, 2022.
Article in English | WPRIM | ID: wpr-928524

ABSTRACT

Ethnicity might be associated with treatment outcomes in advanced prostate cancer. This study aimed to evaluate the efficacy and safety of androgen deprivation therapy (ADT) combined with apalutamide in East Asians with metastatic castration-sensitive prostate cancer (mCSPC). The original phase 3 Targeted Investigational Treatment Analysis of Novel Anti-androgen (TITAN) trial was conducted at 260 sites in 23 countries. This subgroup analysis included patients enrolled in 62 participating centers in China, Japan, and Korea. Radiographic progression-free survival (PFS), time to prostate-specific antigen (PSA) progression, and PSA changes from baseline were compared between groups in the East Asian population. The intent-to-treat East Asian population included 111 and 110 participants in the apalutamide and placebo groups, respectively. The 24-month radiographic PFS rates were 76.1% and 52.3% in the apalutamide and placebo groups, respectively (apalutamide vs placebo: hazard ratio [HR] = 0.506; 95% confidence interval [CI], 0.302-0.849; P = 0.009). Median time to PSA progression was more favorable with apalutamide than placebo (HR = 0.210; 95% CI, 0.124-0.357; P < 0.001). Median maximum percentages of PSA decline from baseline were 99.0% and 73.9% in the apalutamide and placebo groups, respectively. The most common adverse event (AE) was rash in the apalutamide group, with a higher rate than that in the placebo group (37.3% vs 9.1%). The most common grade 3 or 4 AEs were rash (12 [10.9%]) and hypertension (12 [10.9%]) for apalutamide. The efficacy and safety of apalutamide in the East Asian subgroup of the TITAN trial are consistent with the global results.


Subject(s)
Androgen Antagonists/adverse effects , Exanthema/chemically induced , Far East , Humans , Male , Prostate-Specific Antigen , Prostatic Neoplasms, Castration-Resistant/pathology , Thiohydantoins/adverse effects
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